Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009687

Physical Location & Seq

Genomic Coordinates Reverse (-)

44398576 .. 44406687

Gene Structure 8112 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00250580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGAATGCAACAAAGAAGAGGCCCTTAAGGCCAAACAGATAGCAGAGTTGAAGGTTGCAGGGTTTGACTTTGCTGGGGCTAAACGATTTGCTTTAAAAGCTCAAAACTTGTATCCTGAGCTTGATGGTCTTCCTCAATTTCTGGCAACTCTCGATGTCTACACCTCTGCTGAGAAGGGACCAAATGGGGAAGTTAATTGGTACAAGGTCCTTGGAGTGGAACCTTTGGCTAATGAAGATACAATCCAAAAGCAATTCAGGAAACTGGCTCTAATGGTTCACCCAGATAAAAATAAATCTGCAGGTGCTGAAGGGGCGTTCAAGATTATATCCGATGCCAGGAGAATTCTTCATGACCGGAAGCAGATTTTAAGCTCTATGCATGATGGAGTTCCAGATGGGAAATCATCGATGCCTACTACGAAGTATGGTTTCTGCGATAACTTTACTAGGAATCATTCAAGCACAAGGGATGAGAGGAGTGCTACTCCTACCATGCCAGATGGGAAATCATCGATGCCTACTGCACAGAATGGTTTCCAGTTCTTCAAAAGTTATCATTCAAAGACAAGGGACCAGAGGAGTTCTACTCCTACCATGCAAGATGGGAAATCATCGATGCCTACTACTCAGAATAGTTTCCAGTTCAACAATTATAATTCAAACACAAGGGATGAGAGGAGAGCTACTCCTACCAAGCCTTTGTCAACTTCTCGTCCACAGAAAGGTCGGAAAACTACATTTTGGACCATTTGCAGTTGTTGCAAGATGTATTTTGAGTATCTTAGAATGTTTCTCACCCACAAGCTTCAATGTCACAACTGCCATAATCACTTTATAGCTTATAAGATACCAACCCCCAAAAATGCATCCAAGTCAAGGCCTTTTGACCCTCAGCAACAAATATTTAGTCAGCCTACCATTCCCAATAACTCTTGTGATCCAGGAAGGACACCTGCTGCCGCCGCAGATGTGATATCATCAGGAATTTCTGGTGTCAACAAACCAATGTTTGCTCCAGGATGGACACATGCTGCAGCTACAGATGTGAGATCATCAGGAATTTCTGGTGTTGACAAACCAATGTTTGCTCCAGGATGGACACATGCTGCAGCTACAGATGTGAGATCATCAGGAATTTCTGGTGTCGACAAACCAATGCTTGCTCCTGGATGGACACATGCTGCCGCTAGAGATGTGAGATCATCAGGAATTTCTGGTCGATGCTTCAACTCAGCAACTGTTGAAAGTATGCAAGCAGCAGCCTTCAGTTCTAGTCAAGCTGCAGGTAGTGCTCATCCAGCATTCGGGCAATTGAATAGAGGGCATGAAGTGTCATTTCCATATAAGGAGGCCCATCAGTGGAAATTTCATGCTTTTAAGAAACCAGATCCTTTCTTATCTACTGTGTCTCCCAATGCTGGTTTCAGCGCTGTGCCCAATGGAGTTGAGCCTAGATGGGAAAGGCATCGGTGGCAAGAGATGCAAACTAAAGTGGGAATGGGAAATGGAGGAGTTGATATGCCCAATATCTCACGGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3444 DnaJ

Protein Analysis

514

Amino Acids

56.62

Weight (kDa)

9.54

Isoelectric Point (pI)

42.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 122	3.2e-17	DnaJ domain
Zn_ribbon_20	PF23551	248 - 281	7.4e-10	Zinc beta-ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009687)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g08400
malus_domestica	MD06G1141100.v1.1 MD14G1156300.v1.1
prunus_persica	Prupe.5G150300_v2.0.a1
pyrus_communis	pycom14g12990
rosa_chinensis	RchiOBHm_Chr7g0190041
rosa_laevigata	RLG00000003228 RLG00000004569
rosa_multiflora	Rmu_sc0000841.1_g000012 Rmu_sc0004886.1_g000005
rosa_roxburghii	Rroxscaffold_3G00250580 Rroxscaffold_3G00264930
rosa_rugosa	Rorug07G0103200.1
rosa_samantha	Rh7AG107400 Rh7AG237500 Rh7BG109700 Rh7BG233200 Rh7CG112000 Rh7CG253300 Rh7DG110400 Rh7DG243500
rosa_wichuraiana	Rw7G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	657, 846
AarI	CACCTGC	2 cut(s)	293, 964
Acc36I	ACCTGC	3 cut(s)	293, 964, 1277
AccI	GTMKAC	2 cut(s)	159, 1149
AciI	CCGC	3 cut(s)	963, 966, 1188
AclWI	GGATC	2 cut(s)	935, 1385
AcsI	RAATTY	6 cut(s)	345, 987, 1062, 1137, 1212, 1367
AcuI	CTGAAG	2 cut(s)	330, 1252
AfaI	GTAC	1 cut(s)	203
AfeI	AGCGCT	1 cut(s)	1432
AfiI	CCNNNNNNNGG	7 cut(s)	217, 339, 725, 879, 1307, 1362, 1421
AflII	CTTAAG	1 cut(s)	26
AjnI	CCWGG	5 cut(s)	338, 943, 1018, 1093, 1168
AjuI	GAANNNNNNNTTGG	2 cut(s)	1409, 1441
AluBI	AGCT	9 cut(s)	101, 121, 375, 686, 808, 842, 1040, 1115, 1283
AluI	AGCT	9 cut(s)	101, 121, 375, 686, 808, 842, 1040, 1115, 1283
Alw21I	GWGCWC	1 cut(s)	1297
Alw26I	GTCTC	1 cut(s)	1416
AlwI	GGATC	2 cut(s)	935, 1385
AlwNI	CAGNNNCTG	2 cut(s)	308, 1241
Aor51HI	AGCGCT	1 cut(s)	1432
AoxI	GGCC	4 cut(s)	21, 30, 881, 1353
ApeKI	GCWGC	9 cut(s)	959, 1034, 1037, 1109, 1112, 1184, 1259, 1262, 1283
ApoI	RAATTY	6 cut(s)	345, 987, 1062, 1137, 1212, 1367
ArsI	GACNNNNNNTTYG	2 cut(s)	895, 927
Asp700I	GAANNNNTTC	2 cut(s)	536, 638
AspLEI	GCGC	1 cut(s)	1433
AspS9I	GGNCC	6 cut(s)	22, 179, 208, 574, 747, 1354
AsuHPI	GGTGA	2 cut(s)	272, 790
AvaII	GGWCC	4 cut(s)	179, 208, 574, 747
BaeGI	GKGCMC	1 cut(s)	1440
BbsI	GAAGAC	1 cut(s)	122
Bbv12I	GWGCWC	1 cut(s)	1297
BbvCI	CCTCAGC	1 cut(s)	894
BbvI	GCAGC	9 cut(s)	946, 1021, 1049, 1096, 1124, 1171, 1270, 1271, 1274
BciT130I	CCWGG	5 cut(s)	340, 945, 1020, 1095, 1170
BciVI	GTATCC	1 cut(s)	123
BcoDI	GTCTC	1 cut(s)	1416
BfaI	CTAG	4 cut(s)	450, 1191, 1275, 1455
BfmI	CTRYAG	6 cut(s)	300, 1035, 1041, 1110, 1116, 1284
BfoI	RGCGCY	1 cut(s)	1434
BfrI	CTTAAG	1 cut(s)	26
BfuAI	ACCTGC	3 cut(s)	293, 964, 1277
BfuI	GTATCC	1 cut(s)	123
BglI	GCCNNNNNGGC	1 cut(s)	29
Bme1390I	CCNGG	5 cut(s)	340, 945, 1020, 1095, 1170
Bme18I	GGWCC	4 cut(s)	179, 208, 574, 747
BmgT120I	GGNCC	6 cut(s)	22, 179, 208, 574, 747, 1354
BmiI	GGNNCC	3 cut(s)	180, 222, 575
BmrFI	CCNGG	5 cut(s)	340, 945, 1020, 1095, 1170
BmsI	GCATC	8 cut(s)	325, 402, 507, 609, 878, 1214, 1473, 1477
BpiI	GAAGAC	1 cut(s)	122
BplI	GAGNNNNNCTC	6 cut(s)	472, 504, 574, 606, 673, 705
BpmI	CTGGAG	2 cut(s)	1002, 1077
Bpu10I	CCTNAGC	2 cut(s)	117, 894
Bsa29I	ATCGAT	3 cut(s)	410, 515, 617
BsaJI	CCNNGG	1 cut(s)	211
BsaWI	WCCGGW	1 cut(s)	357
BsaXI	ACNNNNNCTCC	2 cut(s)	1396, 1426
Bsc4I	CCNNNNNNNGG	7 cut(s)	217, 339, 725, 879, 1307, 1362, 1421
Bse1I	ACTGG	3 cut(s)	270, 541, 643
BseBI	CCWGG	5 cut(s)	340, 945, 1020, 1095, 1170
BseCI	ATCGAT	3 cut(s)	410, 515, 617
BseDI	CCNNGG	1 cut(s)	211
BseGI	GGATG	7 cut(s)	478, 679, 869, 1028, 1103, 1178, 1297
BseLI	CCNNNNNNNGG	7 cut(s)	217, 339, 725, 879, 1307, 1362, 1421
BseMII	CTCAG	5 cut(s)	108, 162, 644, 908, 1248
BseNI	ACTGG	3 cut(s)	270, 541, 643
BseRI	GAGGAG	4 cut(s)	493, 595, 694, 1527
BseSI	GKGCMC	1 cut(s)	1440
BseXI	GCAGC	9 cut(s)	946, 1021, 1049, 1096, 1124, 1171, 1270, 1271, 1274
BseYI	CCCAGC	1 cut(s)	74
BsgI	GTGCAG	1 cut(s)	510
BshFI	GGCC	4 cut(s)	23, 32, 883, 1355
BshVI	ATCGAT	3 cut(s)	410, 515, 617
BsiHKAI	GWGCWC	1 cut(s)	1297
BsiSI	CCGG	1 cut(s)	358
BslFI	GGGAC	2 cut(s)	192, 587
BslI	CCNNNNNNNGG	7 cut(s)	217, 339, 725, 879, 1307, 1362, 1421
BsmAI	GTCTC	1 cut(s)	1416
BsmFI	GGGAC	2 cut(s)	192, 587
BsmI	GAATGC	2 cut(s)	11, 1304
BsnI	GGCC	4 cut(s)	23, 32, 883, 1355
Bsp1286I	GDGCHC	2 cut(s)	1297, 1440
Bsp143I	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
BspACI	CCGC	3 cut(s)	963, 966, 1188
BspANI	GGCC	4 cut(s)	23, 32, 883, 1355
BspCNI	CTCAG	5 cut(s)	109, 163, 643, 907, 1247
BspDI	ATCGAT	3 cut(s)	410, 515, 617
BspHI	TCATGA	1 cut(s)	352
BspLI	GGNNCC	3 cut(s)	180, 222, 575
BspMAI	CTGCAG	4 cut(s)	304, 1039, 1114, 1288
BspMI	ACCTGC	3 cut(s)	293, 964, 1277
BspPI	GGATC	2 cut(s)	935, 1385
BspTI	CTTAAG	1 cut(s)	26
BsrI	ACTGG	3 cut(s)	270, 541, 643
BssECI	CCNNGG	1 cut(s)	211
BssMI	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
BssT1I	CCWWGG	1 cut(s)	211
Bst2UI	CCWGG	5 cut(s)	340, 945, 1020, 1095, 1170
Bst4CI	ACNGT	2 cut(s)	1243, 1408
Bst6I	CTCTTC	1 cut(s)	12
BstAFI	CTTAAG	1 cut(s)	26
BstAPI	GCANNNNNTGC	3 cut(s)	762, 1292, 1483
BstC8I	GCNNGC	2 cut(s)	1164, 1257
BstDEI	CTNAG	6 cut(s)	117, 171, 630, 785, 894, 1234
BstF5I	GGATG	7 cut(s)	478, 679, 869, 1028, 1103, 1178, 1297
BstH2I	RGCGCY	1 cut(s)	1434
BstHHI	GCGC	1 cut(s)	1433
BstKTI	GATC	5 cut(s)	943, 1055, 1130, 1205, 1393
BstMAI	GTCTC	1 cut(s)	1416
BstMBI	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
BstMWI	GCNNNNNNNGC	9 cut(s)	29, 98, 314, 762, 965, 1292, 1301, 1474, 1483
BstNI	CCWGG	5 cut(s)	340, 945, 1020, 1095, 1170
BstNSI	RCATGY	3 cut(s)	1034, 1109, 1184
BstSCI	CCNGG	5 cut(s)	338, 943, 1018, 1093, 1168
BstSFI	CTRYAG	6 cut(s)	300, 1035, 1041, 1110, 1116, 1284
BstSLI	GKGCMC	1 cut(s)	1440
BstV1I	GCAGC	9 cut(s)	946, 1021, 1049, 1096, 1124, 1171, 1270, 1271, 1274
BstV2I	GAAGAC	1 cut(s)	122
BstX2I	RGATCY	1 cut(s)	1390
BstYI	RGATCY	1 cut(s)	1390
Bsu15I	ATCGAT	3 cut(s)	410, 515, 617
BsuI	GTATCC	1 cut(s)	123
BsuRI	GGCC	4 cut(s)	23, 32, 883, 1355
BsuTUI	ATCGAT	3 cut(s)	410, 515, 617
BtsCI	GGATG	7 cut(s)	478, 679, 869, 1028, 1103, 1178, 1297
BtsIMutI	CAGTG	1 cut(s)	1367
BveI	ACCTGC	3 cut(s)	293, 964, 1277
Cac8I	GCNNGC	2 cut(s)	1164, 1257
CaiI	CAGNNNCTG	2 cut(s)	308, 1241
CciI	TCATGA	1 cut(s)	352
CfoI	GCGC	1 cut(s)	1433
Cfr13I	GGNCC	6 cut(s)	22, 179, 208, 574, 747, 1354
ClaI	ATCGAT	3 cut(s)	410, 515, 617
Csp6I	GTAC	1 cut(s)	202
CviAII	CATG	9 cut(s)	353, 383, 496, 598, 1031, 1106, 1181, 1328, 1373
CviQI	GTAC	1 cut(s)	202
DdeI	CTNAG	6 cut(s)	117, 171, 630, 785, 894, 1234
DpnI	GATC	5 cut(s)	942, 1054, 1129, 1204, 1392
DpnII	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
DraI	TTTAAA	1 cut(s)	96
Eam1104I	CTCTTC	1 cut(s)	12
EarI	CTCTTC	1 cut(s)	12
Eco130I	CCWWGG	1 cut(s)	211
Eco147I	AGGCCT	1 cut(s)	883
Eco32I	GATATC	1 cut(s)	978
Eco47I	GGWCC	4 cut(s)	179, 208, 574, 747
Eco47III	AGCGCT	1 cut(s)	1432
Eco57I	CTGAAG	2 cut(s)	330, 1252
EcoO109I	RGGNCCY	2 cut(s)	22, 208
EcoRI	GAATTC	1 cut(s)	345
EcoRII	CCWGG	5 cut(s)	338, 943, 1018, 1093, 1168
EcoRV	GATATC	1 cut(s)	978
EcoT14I	CCWWGG	1 cut(s)	211
EcoT22I	ATGCAT	2 cut(s)	384, 871
ErhI	CCWWGG	1 cut(s)	211
FaeI	CATG	9 cut(s)	356, 386, 499, 601, 1034, 1109, 1184, 1331, 1376
FalI	AAGNNNNNCTT	2 cut(s)	9, 41
FaqI	GGGAC	2 cut(s)	192, 587
FatI	CATG	9 cut(s)	352, 382, 495, 597, 1030, 1105, 1180, 1327, 1372
FblI	GTMKAC	2 cut(s)	159, 1149
FokI	GGATG	7 cut(s)	485, 686, 856, 1035, 1110, 1185, 1284
FspBI	CTAG	4 cut(s)	450, 1191, 1275, 1455
GlaI	GCGC	1 cut(s)	1432
GsaI	CCCAGC	1 cut(s)	78
GsuI	CTGGAG	2 cut(s)	1002, 1077
HaeII	RGCGCY	1 cut(s)	1434
HaeIII	GGCC	4 cut(s)	23, 32, 883, 1355
HapII	CCGG	1 cut(s)	358
HhaI	GCGC	1 cut(s)	1433
Hin1II	CATG	9 cut(s)	356, 386, 499, 601, 1034, 1109, 1184, 1331, 1376
Hin6I	GCGC	1 cut(s)	1431
HinP1I	GCGC	1 cut(s)	1431
HincII	GTYRAC	4 cut(s)	708, 1000, 1075, 1150
HindII	GTYRAC	4 cut(s)	708, 1000, 1075, 1150
HindIII	AAGCTT	1 cut(s)	806
HinfI	GANTC	1 cut(s)	454
HpaII	CCGG	1 cut(s)	358
HphI	GGTGA	2 cut(s)	272, 790
Hpy166II	GTNNAC	7 cut(s)	160, 280, 708, 719, 1000, 1075, 1150
Hpy188I	TCNGA	3 cut(s)	334, 633, 732
Hpy8I	GTNNAC	7 cut(s)	160, 280, 708, 719, 1000, 1075, 1150
HpyAV	CCTTC	5 cut(s)	46, 169, 305, 942, 1276
HpyCH4III	ACNGT	2 cut(s)	1243, 1408
HpyF10VI	GCNNNNNNNGC	9 cut(s)	29, 98, 314, 762, 965, 1292, 1301, 1474, 1483
HpyF3I	CTNAG	6 cut(s)	117, 171, 630, 785, 894, 1234
Hsp92II	CATG	9 cut(s)	356, 386, 499, 601, 1034, 1109, 1184, 1331, 1376
HspAI	GCGC	1 cut(s)	1431
Kzo9I	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
LmnI	GCTCC	3 cut(s)	1021, 1096, 1171
Lsp1109I	GCAGC	9 cut(s)	946, 1021, 1049, 1096, 1124, 1171, 1270, 1271, 1274
LweI	GCATC	8 cut(s)	325, 402, 507, 609, 878, 1214, 1473, 1477
MaeI	CTAG	4 cut(s)	450, 1191, 1275, 1455
MaeIII	GTNAC	1 cut(s)	815
MalI	GATC	5 cut(s)	942, 1054, 1129, 1204, 1392
MboI	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
MboII	GAAGA	5 cut(s)	29, 122, 248, 341, 538
MfeI	CAATTG	1 cut(s)	1313
MflI	RGATCY	1 cut(s)	1390
MhlI	GDGCHC	2 cut(s)	1297, 1440
MmeI	TCCRAC	1 cut(s)	710
Mph1103I	ATGCAT	2 cut(s)	384, 871
MroXI	GAANNNNTTC	2 cut(s)	536, 638
MseI	TTAA	5 cut(s)	27, 95, 195, 371, 1380
MspCI	CTTAAG	1 cut(s)	26
MspI	CCGG	1 cut(s)	358
MspR9I	CCNGG	5 cut(s)	340, 945, 1020, 1095, 1170
MunI	CAATTG	1 cut(s)	1313
Mva1269I	GAATGC	2 cut(s)	11, 1304
MvaI	CCWGG	5 cut(s)	340, 945, 1020, 1095, 1170
MwoI	GCNNNNNNNGC	9 cut(s)	29, 98, 314, 762, 965, 1292, 1301, 1474, 1483
NdeII	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
NlaIII	CATG	9 cut(s)	356, 386, 499, 601, 1034, 1109, 1184, 1331, 1376
NlaIV	GGNNCC	3 cut(s)	180, 222, 575
NmuCI	GTSAC	1 cut(s)	815
NsiI	ATGCAT	2 cut(s)	384, 871
NspI	RCATGY	3 cut(s)	1034, 1109, 1184
PagI	TCATGA	1 cut(s)	352
PaqCI	CACCTGC	2 cut(s)	293, 964
PceI	AGGCCT	1 cut(s)	883
PctI	GAATGC	2 cut(s)	11, 1304
PdmI	GAANNNNTTC	2 cut(s)	536, 638
PfeI	GAWTC	1 cut(s)	454
PfoI	TCCNGGA	4 cut(s)	943, 1018, 1093, 1168
PpuMI	RGGWCCY	1 cut(s)	208
PsiI	TTATAA	2 cut(s)	657, 846
Psp5II	RGGWCCY	1 cut(s)	208
Psp6I	CCWGG	5 cut(s)	338, 943, 1018, 1093, 1168
PspFI	CCCAGC	1 cut(s)	74
PspGI	CCWGG	5 cut(s)	338, 943, 1018, 1093, 1168
PspN4I	GGNNCC	3 cut(s)	180, 222, 575
PspPI	GGNCC	6 cut(s)	22, 179, 208, 574, 747, 1354
PspPPI	RGGWCCY	1 cut(s)	208
PstI	CTGCAG	4 cut(s)	304, 1039, 1114, 1288
PstNI	CAGNNNCTG	2 cut(s)	308, 1241
PsuI	RGATCY	1 cut(s)	1390
RsaI	GTAC	1 cut(s)	203
RsaNI	GTAC	1 cut(s)	202
SalI	GTCGAC	1 cut(s)	1148
SaqAI	TTAA	5 cut(s)	27, 95, 195, 371, 1380
Sau3AI	GATC	5 cut(s)	940, 1052, 1127, 1202, 1390
Sau96I	GGNCC	6 cut(s)	22, 179, 208, 574, 747, 1354
ScrFI	CCNGG	5 cut(s)	340, 945, 1020, 1095, 1170
SduI	GDGCHC	2 cut(s)	1297, 1440
SfaNI	GCATC	8 cut(s)	325, 402, 507, 609, 878, 1214, 1473, 1477
SfcI	CTRYAG	6 cut(s)	300, 1035, 1041, 1110, 1116, 1284
SfiI	GGCCNNNNNGGCC	1 cut(s)	29
SinI	GGWCC	4 cut(s)	179, 208, 574, 747
SmlI	CTYRAG	1 cut(s)	26
SmoI	CTYRAG	1 cut(s)	26
SseBI	AGGCCT	1 cut(s)	883
SsiI	CCGC	3 cut(s)	963, 966, 1188
SspI	AATATT	1 cut(s)	906
SspMI	CTAG	4 cut(s)	450, 1191, 1275, 1455
StuI	AGGCCT	1 cut(s)	883
StyD4I	CCNGG	5 cut(s)	338, 943, 1018, 1093, 1168
StyI	CCWWGG	1 cut(s)	211
TaaI	ACNGT	2 cut(s)	1243, 1408
TaqI	TCGA	6 cut(s)	153, 410, 515, 617, 1149, 1222
TauI	GCSGC	3 cut(s)	965, 968, 1190
TfiI	GAWTC	1 cut(s)	454
Tru1I	TTAA	5 cut(s)	27, 95, 195, 371, 1380
Tru9I	TTAA	5 cut(s)	27, 95, 195, 371, 1380
TscAI	CASTG	1 cut(s)	1367
TseFI	GTSAC	1 cut(s)	815
TseI	GCWGC	9 cut(s)	959, 1034, 1037, 1109, 1112, 1184, 1259, 1262, 1283
Tsp45I	GTSAC	1 cut(s)	815
TspDTI	ATGAA	4 cut(s)	249, 341, 1344, 1361
TspRI	CASTG	1 cut(s)	1367
Vha464I	CTTAAG	1 cut(s)	26
VpaK11BI	GGWCC	4 cut(s)	179, 208, 574, 747
XapI	RAATTY	6 cut(s)	345, 987, 1062, 1137, 1212, 1367
XceI	RCATGY	3 cut(s)	1034, 1109, 1184
XmiI	GTMKAC	2 cut(s)	159, 1149
XmnI	GAANNNNTTC	2 cut(s)	536, 638
XspI	CTAG	4 cut(s)	450, 1191, 1275, 1455
Zsp2I	ATGCAT	2 cut(s)	384, 871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.