Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009687

Physical Location & Seq

Genomic Coordinates Reverse (-)

58415412 .. 58419628

Gene Structure 4217 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00264930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2517 bp

ATGGAATGCAACAAAGAAGAGGCCCTCAAGGCCAAACAGATAGCAGAGCTGAAGGTTGCAGAGTCTGACTTTAATGGGGCTAAAAGATTTGCTTTAAAAGCTCAAAACTTGTATCCTGAGCTTGATGGTCTTCCTCAATTTCTGGCAACTCTCGATGTCTACACCTCTGCTGAGAAGGGACCAAATGGGGAAGTTAATTGGTACAAGGTCCTCGGTGTGGAACCTTTGGCTGACGAAGACAGAATCCAAAAGCAATTCAGGAACCTGGCTCTCGTTCTACACCCTGACAAAAATAAATCTGCAGGTGCTGAAGGAGCGTTCAAGATTTTATCCGATGCCAGGAGAATTCTTTATGACCAGAAGCAGAATATAAGCTCTATGCATGATGGAATTCCATATGGGTTCCACGATTTGTCTGAGTTTAATCATGCAAACACAAGGGATGAGAGGAGTGCTACTCCTACCATGCCAGATGGGAAATCATCGATGCCTACTACTCAGAATGTTGAGGATGTCTTCAAGAAGAACCATTCAGACACAACGGATGAGAAGAGTGCTACCCCTACCAAGCTTTTGTCAACTTCTCGTCCACAGAAAGGTCGGAAAACTACATTTTGGACCATTTGCAGTTGGTGCAGGATATATTTTGAGTATCTTAGAATGTTTCTCAACCACAAGCTTCAATGTCACAACTGCCAGAAGCATTTTATAGCTTATAAGATACCAGCCCCCAAAAATGCATCCAAGTCAAGTCCTTTTGATGCTCAGCAACATCAAAGTCAGCCTACCATGCCCAATAACTCTTGTGATCCAGGAAGGACACATATTGCCTCTACAGATGTGAGATCATCAGGAATTTCTGGTGTCGACAAACCAATGTTTGCTCCAGGATGGACACATGCTGCTGCTACAGATGTGGGATCATCGGGAATTTCTGGTCTATGCTTCAACTCAGCAAGAGTTCAAAGTGTGCAAGCAACAGCCTCCAGTTCTAGTCAAGCTGCAGGTAGCGCTCATCCCGCATTCAGGCAACTGAATAGAGGGCATGAAGAATCGTTTCCATATAAGGAGGCCCATCAGTGGAAATTTCACGCTTTTAAGAAACCAGATCCTTTCTTACCTACTGGGTCTCCTAATGCTGGTTTCAGAGCTGTGCCCAATGGAGTTAAGCCTAGAAGGAAAAGGCATCGGTGGCAAGAGATGCAAACTAAAGTGGGAATGGGAAACGGAGGAGTTACTATAAACAAGGTATTTGGATCTTCCAAGTTTAGTTTTGGTGCAGGAAGGTTGGATGGAGCTTCAAGGCATAGAGTTAATTGCACAAGAGATTTGTCACATGTAGAACTGCAAAATATATTGGTGAAAAAGGCTGCAAAAGAAATTGTCAAGAAGCTTGATAAGTCGAGCATTCCTTCTGTGCCAAAGACTGCGGACGCTAAGGAGGTAAAGGAAAAGGGAGAGAAAGAAGCTGCTGTGCCTCGTGTTAAAGCCGATATGATAGGGTGTAGGGTATCTGTGGATGATGAGGACATGCCCAGAAAGATGTCCTCCCCTGCCAAGTCTCATGATGACTCTGATGTAGAGGAAGGTAATACAAATTCAATGAGTGTTCCAGACCCTGACTTTCATGATTTTGATAAGGATCGCACGGAAAAGTCATTTGCTAGCAACCAGGTTTGGGCTGTGTATGACGAAGATGATGGCATGCCCCGTTATTATGCTATGGTTCACAGTATCATATCTTTGAAACCATTCAAAATTCAAATCAGTTGGCTTAATTCCAAAACCAATCACGAACTGGCTCCACTAGATTGGGTTGCTTGCGGTTTTCCTAAGACTAGCGGGGACCTCCGGATAGGGAAGCATGGAGTCTACAAGTATCTTCAAACTTTCTCCCACAAGGTTAAGTGGACAAAAGGCAGAAGAGGAGCAGTTCGTATATATCCTGGAAAGGGAGATGTCTGGGCTTTATATAGGAACTGGTCCCCTGATTGGGATGAACATACCCCAGATGAAGTGATACACAAATATGACATGGTGCAAGTGCTAGAAGATTATAATGAGGAGAGAGGTGTGAGCATTCTCCCTCTTGTTAAAGTTGCTGGGTTCAAGACTGTGTTCCGCCAACATTCAGACAGAAGTAGAATCAGGACAATTCCAAGAGAAGAAATGTTTCGTTTCTCTCACCAGGTCCCTTATTACTTGCTCACAGGTCTTGAAGGAGAGAATGCTCCCAAGGGTTGCTTGGAGCTTGATCCTGCATCTACTCCTTTGGAACTTCTTCAGATAAAGTCCGAAGCACAAGCCAAAATGGAGGAGACAGCTGGAAAATGTAATGGAGAAGAGAATGGCAAAGTAACTATAGAAACAGGTCTGGTAGAAGATGCTGAGGAAAAAGATCCGGCAGACTTGATGAAGAGGCAGAAGGATCCCCAGGCAACAAAGTGGCGGGTATATAGTCGGAAACGCCCGAGGGAAGGACAAGCTGTCGAGGGTGCTAAGCGGTTGCGGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3444 DnaJ

Protein Analysis

838

Amino Acids

94.16

Weight (kDa)

9.01

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 122	1.2e-15	DnaJ domain
Zn_ribbon_20	PF23551	205 - 238	4e-09	Zinc beta-ribbon domain
DUF3444	PF11926	532 - 738	4.1e-78	Domain of unknown function (DUF3444)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009687)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g08400
malus_domestica	MD06G1141100.v1.1 MD14G1156300.v1.1
prunus_persica	Prupe.5G150300_v2.0.a1
pyrus_communis	pycom14g12990
rosa_chinensis	RchiOBHm_Chr7g0190041
rosa_laevigata	RLG00000003228 RLG00000004569
rosa_multiflora	Rmu_sc0000841.1_g000012 Rmu_sc0004886.1_g000005
rosa_roxburghii	Rroxscaffold_3G00250580 Rroxscaffold_3G00264930
rosa_rugosa	Rorug07G0103200.1
rosa_samantha	Rh7AG107400 Rh7AG237500 Rh7BG109700 Rh7BG233200 Rh7CG112000 Rh7CG253300 Rh7DG110400 Rh7DG243500
rosa_wichuraiana	Rw7G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	717, 2058
AarI	CACCTGC	1 cut(s)	293
Acc36I	ACCTGC	2 cut(s)	293, 995
AccI	GTMKAC	3 cut(s)	159, 867, 1872
AccIII	TCCGGA	1 cut(s)	1851
AciI	CCGC	8 cut(s)	1020, 1430, 1824, 1842, 2122, 2449, 2503, 2509
AclWI	GGATC	9 cut(s)	803, 928, 1103, 1264, 1650, 2249, 2393, 2423, 2436
AcsI	RAATTY	7 cut(s)	345, 390, 855, 930, 1085, 1597, 1758
AcuI	CTGAAG	3 cut(s)	71, 330, 2267
AfaI	GTAC	1 cut(s)	203
AfeI	AGCGCT	1 cut(s)	1012
AflIII	ACRYGT	1 cut(s)	1336
AhdI	GACNNNNNGTC	1 cut(s)	2486
AjnI	CCWGG	8 cut(s)	264, 338, 811, 886, 1671, 1945, 2187, 2433
Alw26I	GTCTC	3 cut(s)	1134, 1566, 2312
AlwI	GGATC	9 cut(s)	803, 928, 1103, 1264, 1650, 2249, 2393, 2423, 2436
AlwNI	CAGNNNCTG	3 cut(s)	65, 308, 1619
Ama87I	CYCGRG	1 cut(s)	2470
Aor13HI	TCCGGA	1 cut(s)	1851
Aor51HI	AGCGCT	1 cut(s)	1012
AoxI	GGCC	3 cut(s)	21, 30, 1071
ApeKI	GCWGC	5 cut(s)	902, 905, 1001, 1370, 1469
ApoI	RAATTY	7 cut(s)	345, 390, 855, 930, 1085, 1597, 1758
Asp700I	GAANNNNTTC	4 cut(s)	1056, 1751, 2172, 2280
AspLEI	GCGC	1 cut(s)	1013
AspS9I	GGNCC	8 cut(s)	22, 179, 208, 618, 1072, 1846, 1983, 2191
AsuHPI	GGTGA	2 cut(s)	1372, 2177
AsuNHI	GCTAGC	1 cut(s)	1664
AvaI	CYCGRG	1 cut(s)	2470
AvaII	GGWCC	6 cut(s)	179, 208, 618, 1846, 1983, 2191
BaeGI	GKGCMC	1 cut(s)	1158
BamHI	GGATCC	1 cut(s)	2428
BauI	CACGAG	1 cut(s)	1479
BbsI	GAAGAC	3 cut(s)	122, 243, 508
BbvCI	CCTCAGC	1 cut(s)	2388
BbvI	GCAGC	5 cut(s)	889, 892, 988, 1357, 1456
BccI	CCATC	7 cut(s)	119, 380, 467, 885, 1083, 1286, 1694
BciT130I	CCWGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
BciVI	GTATCC	1 cut(s)	123
BcoDI	GTCTC	3 cut(s)	1134, 1566, 2312
BfaI	CTAG	6 cut(s)	993, 1173, 1665, 1808, 1839, 2048
BfmI	CTRYAG	5 cut(s)	300, 834, 909, 1002, 2361
BfoI	RGCGCY	1 cut(s)	1014
BfuAI	ACCTGC	2 cut(s)	293, 995
BfuI	GTATCC	1 cut(s)	123
BglI	GCCNNNNNGGC	1 cut(s)	29
BisI	GCNGC	6 cut(s)	903, 906, 1002, 1371, 1470, 2510
BlpI	GCTNAGC	2 cut(s)	765, 2499
BlsI	GCNGC	6 cut(s)	904, 907, 1003, 1372, 1471, 2511
Bme1390I	CCNGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
Bme18I	GGWCC	6 cut(s)	179, 208, 618, 1846, 1983, 2191
BmeRI	GACNNNNNGTC	1 cut(s)	2486
BmeT110I	CYCGRG	1 cut(s)	2470
BmgT120I	GGNCC	8 cut(s)	22, 179, 208, 618, 1072, 1846, 1983, 2191
BmiI	GGNNCC	9 cut(s)	180, 222, 263, 404, 1803, 1847, 1985, 2193, 2430
BmrFI	CCNGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
BmrI	ACTGGG	1 cut(s)	1134
BmsI	GCATC	8 cut(s)	325, 477, 749, 751, 1191, 1195, 2270, 2374
BmtI	GCTAGC	1 cut(s)	1668
BmuI	ACTGGG	1 cut(s)	1134
BpiI	GAAGAC	3 cut(s)	122, 243, 508
BplI	GAGNNNNNCTC	2 cut(s)	442, 474
BpmI	CTGGAG	2 cut(s)	870, 970
Bpu10I	CCTNAGC	3 cut(s)	117, 1437, 2388
Bpu1102I	GCTNAGC	2 cut(s)	765, 2499
BpuEI	CTTGAG	1 cut(s)	11
Bsa29I	ATCGAT	1 cut(s)	485
BsaBI	GATNNNNATC	1 cut(s)	1641
BsaI	GGTCTC	1 cut(s)	1134
BsaJI	CCNNGG	4 cut(s)	211, 2235, 2433, 2471
BsaWI	WCCGGW	1 cut(s)	1851
BsaXI	ACNNNNNCTCC	2 cut(s)	1114, 1144
Bse1I	ACTGG	4 cut(s)	987, 1129, 1803, 1985
Bse8I	GATNNNNATC	1 cut(s)	1641
BseAI	TCCGGA	1 cut(s)	1851
BseBI	CCWGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
BseCI	ATCGAT	1 cut(s)	485
BseDI	CCNNGG	4 cut(s)	211, 2235, 2433, 2471
BseGI	GGATG	9 cut(s)	448, 517, 550, 740, 896, 1015, 1297, 1525, 2002
BseJI	GATNNNNATC	1 cut(s)	1641
BseMII	CTCAG	7 cut(s)	108, 162, 408, 512, 779, 966, 2379
BseNI	ACTGG	4 cut(s)	987, 1129, 1803, 1985
BseRI	GAGGAG	5 cut(s)	463, 1245, 1941, 2078, 2330
BseSI	GKGCMC	1 cut(s)	1158
BseXI	GCAGC	5 cut(s)	889, 892, 988, 1357, 1456
BseYI	CCCAGC	1 cut(s)	2102
BsgI	GTGCAG	2 cut(s)	655, 1299
BshFI	GGCC	3 cut(s)	23, 32, 1073
BshVI	ATCGAT	1 cut(s)	485
BsiHKCI	CYCGRG	1 cut(s)	2470
BsiSI	CCGG	2 cut(s)	1852, 2402
BslFI	GGGAC	4 cut(s)	192, 1859, 1969, 2177
BsmAI	GTCTC	3 cut(s)	1134, 1566, 2312
BsmFI	GGGAC	4 cut(s)	192, 1859, 1969, 2177
BsmI	GAATGC	5 cut(s)	11, 1022, 1407, 2079, 2233
BsnI	GGCC	3 cut(s)	23, 32, 1073
Bso31I	GGTCTC	1 cut(s)	1134
BsoBI	CYCGRG	1 cut(s)	2470
Bsp1286I	GDGCHC	1 cut(s)	1158
Bsp13I	TCCGGA	1 cut(s)	1851
Bsp143I	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
Bsp1720I	GCTNAGC	2 cut(s)	765, 2499
BspACI	CCGC	8 cut(s)	1020, 1430, 1824, 1842, 2122, 2449, 2503, 2509
BspANI	GGCC	3 cut(s)	23, 32, 1073
BspCNI	CTCAG	7 cut(s)	109, 163, 409, 511, 778, 965, 2380
BspDI	ATCGAT	1 cut(s)	485
BspEI	TCCGGA	1 cut(s)	1851
BspHI	TCATGA	2 cut(s)	1564, 1627
BspLI	GGNNCC	9 cut(s)	180, 222, 263, 404, 1803, 1847, 1985, 2193, 2430
BspMAI	CTGCAG	2 cut(s)	304, 1006
BspMI	ACCTGC	2 cut(s)	293, 995
BspOI	GCTAGC	1 cut(s)	1668
BspPI	GGATC	9 cut(s)	803, 928, 1103, 1264, 1650, 2249, 2393, 2423, 2436
BspTNI	GGTCTC	1 cut(s)	1134
BsrI	ACTGG	4 cut(s)	987, 1129, 1803, 1985
BssECI	CCNNGG	4 cut(s)	211, 2235, 2433, 2471
BssMI	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
BssSI	CACGAG	1 cut(s)	1479
BssT1I	CCWWGG	1 cut(s)	2235
Bst2BI	CACGAG	1 cut(s)	1479
Bst2UI	CCWGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
Bst4CI	ACNGT	2 cut(s)	1733, 2116
Bst6I	CTCTTC	5 cut(s)	12, 545, 1918, 2337, 2411
BstAPI	GCANNNNNTGC	2 cut(s)	633, 1201
BstC8I	GCNNGC	4 cut(s)	975, 1666, 1706, 1822
BstF5I	GGATG	9 cut(s)	448, 517, 550, 740, 896, 1015, 1297, 1525, 2002
BstH2I	RGCGCY	1 cut(s)	1014
BstHHI	GCGC	1 cut(s)	1013
BstKTI	GATC	9 cut(s)	811, 848, 923, 1111, 1259, 1645, 2257, 2401, 2431
BstMAI	GTCTC	3 cut(s)	1134, 1566, 2312
BstMBI	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
BstNI	CCWGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
BstNSI	RCATGY	4 cut(s)	902, 1340, 1534, 1708
BstSCI	CCNGG	8 cut(s)	264, 338, 811, 886, 1671, 1945, 2187, 2433
BstSFI	CTRYAG	5 cut(s)	300, 834, 909, 1002, 2361
BstSLI	GKGCMC	1 cut(s)	1158
BstV1I	GCAGC	5 cut(s)	889, 892, 988, 1357, 1456
BstV2I	GAAGAC	3 cut(s)	122, 243, 508
BstX2I	RGATCY	4 cut(s)	1108, 1256, 2398, 2428
BstXI	CCANNNNNNTGG	1 cut(s)	1812
BstYI	RGATCY	4 cut(s)	1108, 1256, 2398, 2428
Bsu15I	ATCGAT	1 cut(s)	485
BsuI	GTATCC	1 cut(s)	123
BsuRI	GGCC	3 cut(s)	23, 32, 1073
BsuTUI	ATCGAT	1 cut(s)	485
BtsCI	GGATG	9 cut(s)	448, 517, 550, 740, 896, 1015, 1297, 1525, 2002
BtsIMutI	CAGTG	1 cut(s)	1085
BveI	ACCTGC	2 cut(s)	293, 995
Cac8I	GCNNGC	4 cut(s)	975, 1666, 1706, 1822
CaiI	CAGNNNCTG	3 cut(s)	65, 308, 1619
CciI	TCATGA	2 cut(s)	1564, 1627
CfoI	GCGC	1 cut(s)	1013
Cfr13I	GGNCC	8 cut(s)	22, 179, 208, 618, 1072, 1846, 1983, 2191
ClaI	ATCGAT	1 cut(s)	485
CseI	GACGC	1 cut(s)	1442
CsiI	ACCWGGT	2 cut(s)	1671, 2187
Csp6I	GTAC	1 cut(s)	202
CviQI	GTAC	1 cut(s)	202
DpnI	GATC	9 cut(s)	810, 847, 922, 1110, 1258, 1644, 2256, 2400, 2430
DpnII	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
DraI	TTTAAA	1 cut(s)	96
DriI	GACNNNNNGTC	1 cut(s)	2486
Eam1104I	CTCTTC	5 cut(s)	12, 545, 1918, 2337, 2411
Eam1105I	GACNNNNNGTC	1 cut(s)	2486
EarI	CTCTTC	5 cut(s)	12, 545, 1918, 2337, 2411
EciI	GGCGGA	1 cut(s)	2111
Eco130I	CCWWGG	1 cut(s)	2235
Eco31I	GGTCTC	1 cut(s)	1134
Eco47I	GGWCC	6 cut(s)	179, 208, 618, 1846, 1983, 2191
Eco47III	AGCGCT	1 cut(s)	1012
Eco57I	CTGAAG	3 cut(s)	71, 330, 2267
Eco88I	CYCGRG	1 cut(s)	2470
EcoO109I	RGGNCCY	4 cut(s)	22, 208, 1846, 2191
EcoRI	GAATTC	2 cut(s)	345, 390
EcoRII	CCWGG	8 cut(s)	264, 338, 811, 886, 1671, 1945, 2187, 2433
EcoT14I	CCWWGG	1 cut(s)	2235
EcoT22I	ATGCAT	2 cut(s)	384, 742
ErhI	CCWWGG	1 cut(s)	2235
FalI	AAGNNNNNCTT	4 cut(s)	76, 108, 2228, 2260
FaqI	GGGAC	4 cut(s)	192, 1859, 1969, 2177
FauI	CCCGC	3 cut(s)	1027, 1835, 2442
FauNDI	CATATG	1 cut(s)	397
FblI	GTMKAC	3 cut(s)	159, 867, 1872
Fnu4HI	GCNGC	6 cut(s)	903, 906, 1002, 1371, 1470, 2510
FokI	GGATG	9 cut(s)	455, 524, 557, 727, 903, 1002, 1304, 1532, 2009
Fsp4HI	GCNGC	6 cut(s)	903, 906, 1002, 1371, 1470, 2510
FspBI	CTAG	6 cut(s)	993, 1173, 1665, 1808, 1839, 2048
GlaI	GCGC	1 cut(s)	1012
GluI	GCNGC	6 cut(s)	903, 906, 1002, 1371, 1470, 2510
GsaI	CCCAGC	1 cut(s)	2106
GsuI	CTGGAG	2 cut(s)	870, 970
HaeII	RGCGCY	1 cut(s)	1014
HaeIII	GGCC	3 cut(s)	23, 32, 1073
HapII	CCGG	2 cut(s)	1852, 2402
HgaI	GACGC	1 cut(s)	1442
HhaI	GCGC	1 cut(s)	1013
Hin6I	GCGC	1 cut(s)	1011
HinP1I	GCGC	1 cut(s)	1011
HincII	GTYRAC	2 cut(s)	579, 868
HindII	GTYRAC	2 cut(s)	579, 868
HindIII	AAGCTT	3 cut(s)	569, 677, 1391
HinfI	GANTC	6 cut(s)	62, 243, 1052, 1571, 1869, 2145
HpaII	CCGG	2 cut(s)	1852, 2402
HphI	GGTGA	2 cut(s)	1372, 2177
Hpy166II	GTNNAC	7 cut(s)	160, 579, 590, 868, 1729, 1873, 1911
Hpy8I	GTNNAC	7 cut(s)	160, 579, 590, 868, 1729, 1873, 1911
HpyCH4III	ACNGT	2 cut(s)	1733, 2116
HspAI	GCGC	1 cut(s)	1011
Kpn2I	TCCGGA	1 cut(s)	1851
Kzo9I	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
LmnI	GCTCC	7 cut(s)	314, 889, 1295, 1807, 1928, 2236, 2248
Lsp1109I	GCAGC	5 cut(s)	889, 892, 988, 1357, 1456
LweI	GCATC	8 cut(s)	325, 477, 749, 751, 1191, 1195, 2270, 2374
MabI	ACCWGGT	2 cut(s)	1671, 2187
MaeI	CTAG	6 cut(s)	993, 1173, 1665, 1808, 1839, 2048
MaeIII	GTNAC	4 cut(s)	686, 1234, 1332, 2356
MalI	GATC	9 cut(s)	810, 847, 922, 1110, 1258, 1644, 2256, 2400, 2430
MboI	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
MflI	RGATCY	4 cut(s)	1108, 1256, 2398, 2428
MhlI	GDGCHC	1 cut(s)	1158
MlyI	GAGTC	3 cut(s)	71, 1565, 1878
MmeI	TCCRAC	3 cut(s)	581, 1269, 2441
Mph1103I	ATGCAT	2 cut(s)	384, 742
MroI	TCCGGA	1 cut(s)	1851
MroXI	GAANNNNTTC	4 cut(s)	1056, 1751, 2172, 2280
MslI	CAYNNNNRTG	1 cut(s)	2028
MspA1I	CMGCKG	1 cut(s)	2324
MspI	CCGG	2 cut(s)	1852, 2402
MspR9I	CCNGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
Mva1269I	GAATGC	5 cut(s)	11, 1022, 1407, 2079, 2233
MvaI	CCWGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
NdeI	CATATG	1 cut(s)	397
NdeII	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
NheI	GCTAGC	1 cut(s)	1664
NlaIV	GGNNCC	9 cut(s)	180, 222, 263, 404, 1803, 1847, 1985, 2193, 2430
NmuCI	GTSAC	2 cut(s)	686, 1332
NsiI	ATGCAT	2 cut(s)	384, 742
NspI	RCATGY	4 cut(s)	902, 1340, 1534, 1708
PaeI	GCATGC	1 cut(s)	1708
PagI	TCATGA	2 cut(s)	1564, 1627
PaqCI	CACCTGC	1 cut(s)	293
PciI	ACATGT	1 cut(s)	1336
PctI	GAATGC	5 cut(s)	11, 1022, 1407, 2079, 2233
PdmI	GAANNNNTTC	4 cut(s)	1056, 1751, 2172, 2280
PfeI	GAWTC	3 cut(s)	243, 1052, 2145
PfoI	TCCNGGA	3 cut(s)	811, 886, 1945
PkrI	GCNGC	6 cut(s)	904, 907, 1003, 1372, 1471, 2511
PleI	GAGTC	3 cut(s)	70, 1565, 1877
PpsI	GAGTC	3 cut(s)	70, 1565, 1877
PpuMI	RGGWCCY	3 cut(s)	208, 1846, 2191
PscI	ACATGT	1 cut(s)	1336
PsiI	TTATAA	2 cut(s)	717, 2058
Psp5II	RGGWCCY	3 cut(s)	208, 1846, 2191
Psp6I	CCWGG	8 cut(s)	264, 338, 811, 886, 1671, 1945, 2187, 2433
PspFI	CCCAGC	1 cut(s)	2102
PspGI	CCWGG	8 cut(s)	264, 338, 811, 886, 1671, 1945, 2187, 2433
PspN4I	GGNNCC	9 cut(s)	180, 222, 263, 404, 1803, 1847, 1985, 2193, 2430
PspPI	GGNCC	8 cut(s)	22, 179, 208, 618, 1072, 1846, 1983, 2191
PspPPI	RGGWCCY	3 cut(s)	208, 1846, 2191
PstI	CTGCAG	2 cut(s)	304, 1006
PstNI	CAGNNNCTG	3 cut(s)	65, 308, 1619
PsuI	RGATCY	4 cut(s)	1108, 1256, 2398, 2428
PvuII	CAGCTG	1 cut(s)	2324
RsaI	GTAC	1 cut(s)	203
RsaNI	GTAC	1 cut(s)	202
RseI	CAYNNNNRTG	1 cut(s)	2028
SalI	GTCGAC	1 cut(s)	866
SatI	GCNGC	6 cut(s)	903, 906, 1002, 1371, 1470, 2510
Sau3AI	GATC	9 cut(s)	808, 845, 920, 1108, 1256, 1642, 2254, 2398, 2428
Sau96I	GGNCC	8 cut(s)	22, 179, 208, 618, 1072, 1846, 1983, 2191
SchI	GAGTC	3 cut(s)	71, 1565, 1878
ScrFI	CCNGG	8 cut(s)	266, 340, 813, 888, 1673, 1947, 2189, 2435
SduI	GDGCHC	1 cut(s)	1158
SexAI	ACCWGGT	2 cut(s)	1671, 2187
SfaNI	GCATC	8 cut(s)	325, 477, 749, 751, 1191, 1195, 2270, 2374
SfcI	CTRYAG	5 cut(s)	300, 834, 909, 1002, 2361
SfiI	GGCCNNNNNGGCC	1 cut(s)	29
SinI	GGWCC	6 cut(s)	179, 208, 618, 1846, 1983, 2191
SmiMI	CAYNNNNRTG	1 cut(s)	2028
SmlI	CTYRAG	1 cut(s)	26
SmoI	CTYRAG	1 cut(s)	26
SphI	GCATGC	1 cut(s)	1708
SsiI	CCGC	8 cut(s)	1020, 1430, 1824, 1842, 2122, 2449, 2503, 2509
SspMI	CTAG	6 cut(s)	993, 1173, 1665, 1808, 1839, 2048
StyD4I	CCNGG	8 cut(s)	264, 338, 811, 886, 1671, 1945, 2187, 2433
StyI	CCWWGG	1 cut(s)	2235
TaaI	ACNGT	2 cut(s)	1733, 2116
TaqI	TCGA	5 cut(s)	153, 485, 867, 1403, 2490
TauI	GCSGC	1 cut(s)	2512
TfiI	GAWTC	3 cut(s)	243, 1052, 2145
TscAI	CASTG	1 cut(s)	1085
TseFI	GTSAC	2 cut(s)	686, 1332
TseI	GCWGC	5 cut(s)	902, 905, 1001, 1370, 1469
Tsp45I	GTSAC	2 cut(s)	686, 1332
TspDTI	ATGAA	5 cut(s)	1062, 1616, 2013, 2028, 2429
TspGWI	ACGGA	3 cut(s)	557, 1242, 1664
TspRI	CASTG	1 cut(s)	1085
VpaK11BI	GGWCC	6 cut(s)	179, 208, 618, 1846, 1983, 2191
XapI	RAATTY	7 cut(s)	345, 390, 855, 930, 1085, 1597, 1758
XceI	RCATGY	4 cut(s)	902, 1340, 1534, 1708
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1795, 2242
XmiI	GTMKAC	3 cut(s)	159, 867, 1872
XmnI	GAANNNNTTC	4 cut(s)	1056, 1751, 2172, 2280
XspI	CTAG	6 cut(s)	993, 1173, 1665, 1808, 1839, 2048
Zsp2I	ATGCAT	2 cut(s)	384, 742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.