Rroxscaffold_3G00264490

Nucleolar protein 56-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014044
Physical Location & Seq
Reverse (-)
58125744 .. 58129158
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264490.1

Sequence Viewer

Length: 1650 bp
ATGGCGGTTTACCTGCTCTACGAAGCAGCTTCCGGTTACTCTCTATTCCTAGCTCACGGCCTCGACCAGATCGGCCAGAACACCGAGGCGGTTCGGAGCTCCGTTTCGGACCTGAACCGGTTCGGGAAGGTGGTCCAGCTCGCGGCTTTTCACCCATTTGAGCAGGCCCTCGATGCCCTTAACCAGTGCAACTCAGTCTCCGAAGGGATTATGACTGATGAGTTGAGGAACTTTTTGGAACTTAATCTCCCAAAAGTAAAGGAAGGCAAGAAGCCAAAGTTCAGTTTGGGTGTATATGAACCCAAGCTCGGTTCTCACATATTTGAAGTGACTAAAATCCCCTGCCAAAGCAATGAGTTTGTTCTTGAGCTACTTCGTGGAGTTCGGCTTCATTTTGATAGGTTTATCAAGGACCTAAAGCCCGGAGACTTAGAGAAAGCCCAGCTTGGTCTCAGCCACAGTTACAGCAGGTCCAAGGTCAAGTTTAATGTTAACCGTGTTGACAATATGGTGATTCAAGCAATCTTCCTTTTAGATACCCTCGATAAGGATGTGAATTCTTTCTCAATGAGAGTCAGAGAATGGTACTCTTGGCATTTTCCTGAATTGGTGAAGATTGTCAATGACAATTATCTCTATGCTAAACTTGCAAAAACTATAAGAGATAAGTCAACTTTGTCTGAAGACAACCTCGAAGAATTAACTGATATTGTTGGTGATGAAGATAAGGCAAAGGAGATCATTGAAGCTGCCAAAGCATCCATGGGGCAGGATTTGTCTGATGTTGATTTGCTTAATGTTCAACAATTTGCACAAAAAGTGATGAACCTCTCAGAGTATAGGAAAAAACTGTATGACTATCTGGTCACTAAGATGAATGATATTGCACCCAATTTGGCCTCTTTAATCGGGGAAGTAGTAGGAGCACGTTTAATTTCTCATGCGGGTAGTCTTACAAACTTGGCTAAATGCCCTTCTTCTACCCTTCAGATCCTTGGTGCCGAGAAGGCCCTCTTCAGGGCCTTGAAAACCAAGGGAAACACACCCAAGTATGGACTGATATTCCATTCATCTTTTATTGGTCGCGCATCTGCCCGGAATAAGGGCAGAATGGCTCGTTATCTTGCAAACAAGTGTTCTATTGCTTCTCGCATTGACTGCTTTGCAGATAATAGCACTACTGCTTTTGGGGAGAAACTCCGCGAACAAGTTGAGGAGCGTCTTGACTTTTATGACAAGGGTGTTGCACCTCGTAAAAACATTGATGTGATGAAATCTGCGATGGAGAGTACTCAAAGCAAAGATACAGATATGGAAACAGAAGAAGTGACTGTTGAAGCTTCAGAAAAGAAAAGCAAGAAGAAATCAAAGAGTGCAGCTACTGAGAATGGTGATGTGGATGAACCAGCTACAAATGGAGATGCTACTGAGGGTAAATCTGAAAAGAAGAAGAAGAAGAAGGACAAGAGGAAATTGGATAAGGAAGATGAAGAGATGGAGGATTCAAACAATGGACATGCAGAGGAGGATGGAACAAGCAAGAAGAAGAAGAAGAAGAAAAGTAAACATGGAGAGGCTGATGGAGCCAATTTGGCCAGTGATGTTAAGAAGAAGAAAAAGAAGAAATCTAAAAGTGAAGACTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOP5NT Nop

Protein Analysis

549

Amino Acids

61.48

Weight (kDa)

8.64

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOP5NT PF08156 4 - 69 9e-21 NOP5NT (NUC127) domain
Nop PF01798 177 - 409 3.9e-89 snoRNA binding domain, fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56110 AT3G12860
fragaria_vesca FvH4_5g07871
malus_domestica MD06G1145200.v1.1 MD14G1159900.v1.1
prunus_persica Prupe.5G153500_v2.0.a1 Prupe.5G153500_v2.0.a1
pyrus_communis pycom06g13450
rosa_chinensis RchiOBHm_Chr7g0190581
rosa_laevigata RLG00000004513
rosa_multiflora Rmu_sc0036974.1_g000001
rosa_roxburghii Rroxscaffold_3G00264490
rosa_rugosa Rorug06G0505400
rosa_samantha Rh7BG114800 Rh7CG116900 Rh7DG116000
rosa_wichuraiana Rw7G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 863
Acc36I ACCTGC 2 cut(s) 21, 459
AccB1I GGYRCC 1 cut(s) 998
AccII CGCG 3 cut(s) 143, 1086, 1203
AciI CCGC 5 cut(s) 5, 89, 143, 944, 1201
AclWI GGATC 1 cut(s) 985
AcoI YGGCCR 2 cut(s) 73, 1593
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 4 cut(s) 702, 971, 1000, 1326
AfaI GTAC 2 cut(s) 587, 1291
AfiI CCNNNNNNNGG 7 cut(s) 142, 308, 547, 1017, 1018, 1052, 1102
AgeI ACCGGT 1 cut(s) 117
AgsI TTSAA 7 cut(s) 326, 518, 746, 803, 1027, 1337, 1506
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 2 cut(s) 101, 928
Alw26I GTCTC 3 cut(s) 202, 420, 455
AlwI GGATC 1 cut(s) 985
AlwNI CAGNNNCTG 1 cut(s) 1382
AoxI GGCC 7 cut(s) 58, 73, 165, 897, 1008, 1020, 1593
ApeKI GCWGC 3 cut(s) 26, 749, 1376
ApoI RAATTY 1 cut(s) 556
AsiGI ACCGGT 1 cut(s) 117
Asp700I GAANNNNTTC 2 cut(s) 119, 560
AspLEI GCGC 1 cut(s) 1088
AspS9I GGNCC 7 cut(s) 109, 133, 166, 412, 471, 1009, 1020
AsuC2I CCSGG 2 cut(s) 423, 1096
AsuHPI GGTGA 5 cut(s) 143, 523, 622, 728, 1403
AvaII GGWCC 4 cut(s) 109, 133, 412, 471
BalI TGGCCA 1 cut(s) 1595
BanI GGYRCC 1 cut(s) 998
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 2 cut(s) 690, 1644
Bbv12I GWGCWC 2 cut(s) 101, 928
BbvI GCAGC 3 cut(s) 38, 736, 1388
BccI CCATC 4 cut(s) 1276, 1489, 1523, 1574
BceAI ACGGC 1 cut(s) 73
BcnI CCSGG 2 cut(s) 423, 1096
BcoDI GTCTC 3 cut(s) 202, 420, 455
BfaI CTAG 1 cut(s) 50
BfuAI ACCTGC 2 cut(s) 21, 459
BglI GCCNNNNNGGC 2 cut(s) 1007, 1592
BisI GCNGC 4 cut(s) 27, 144, 750, 1377
BlsI GCNGC 4 cut(s) 28, 145, 751, 1378
BmcAI AGTACT 1 cut(s) 1291
Bme1390I CCNGG 2 cut(s) 423, 1096
Bme18I GGWCC 4 cut(s) 109, 133, 412, 471
BmgT120I GGNCC 7 cut(s) 109, 133, 166, 412, 471, 1009, 1020
BmiI GGNNCC 2 cut(s) 1000, 1585
BmrFI CCNGG 2 cut(s) 423, 1096
BmsI GCATC 4 cut(s) 163, 767, 1097, 1411
BpiI GAAGAC 2 cut(s) 690, 1644
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 2 cut(s) 423, 1096
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 5 cut(s) 84, 474, 762, 994, 1032
BsaWI WCCGGW 2 cut(s) 32, 117
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 231, 261
Bsc4I CCNNNNNNNGG 7 cut(s) 142, 308, 547, 1017, 1018, 1052, 1102
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 184, 1596
Bse3DI GCAATG 1 cut(s) 358
BseDI CCNNGG 5 cut(s) 84, 474, 762, 994, 1032
BseGI GGATG 4 cut(s) 556, 758, 1405, 1534
BseLI CCNNNNNNNGG 7 cut(s) 142, 308, 547, 1017, 1018, 1052, 1102
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 5 cut(s) 207, 466, 846, 1374, 1419
BseNI ACTGG 2 cut(s) 184, 1596
BseRI GAGGAG 2 cut(s) 1229, 1538
BseXI GCAGC 3 cut(s) 38, 736, 1388
BseYI CCCAGC 1 cut(s) 441
BsgI GTGCAG 1 cut(s) 1395
Bsh1236I CGCG 3 cut(s) 143, 1086, 1203
BshFI GGCC 7 cut(s) 60, 75, 167, 899, 1010, 1022, 1595
BshNI GGYRCC 1 cut(s) 998
BshTI ACCGGT 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 101, 928
BsiSI CCGG 4 cut(s) 33, 118, 423, 1096
BslI CCNNNNNNNGG 7 cut(s) 142, 308, 547, 1017, 1018, 1052, 1102
BsmAI GTCTC 3 cut(s) 202, 420, 455
BsnI GGCC 7 cut(s) 60, 75, 167, 899, 1010, 1022, 1595
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 2 cut(s) 101, 928
Bsp143I GATC 3 cut(s) 69, 738, 990
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 5 cut(s) 5, 89, 143, 944, 1201
BspANI GGCC 7 cut(s) 60, 75, 167, 899, 1010, 1022, 1595
BspCNI CTCAG 5 cut(s) 206, 465, 845, 1375, 1420
BspFNI CGCG 3 cut(s) 143, 1086, 1203
BspLI GGNNCC 2 cut(s) 1000, 1585
BspMI ACCTGC 2 cut(s) 21, 459
BspPI GGATC 1 cut(s) 985
BspT107I GGYRCC 1 cut(s) 998
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 358
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 2 cut(s) 184, 1596
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 5 cut(s) 84, 474, 762, 994, 1032
BssMI GATC 3 cut(s) 69, 738, 990
BssT1I CCWWGG 4 cut(s) 474, 762, 994, 1032
Bst4CI ACNGT 4 cut(s) 461, 497, 852, 1333
Bst6I CTCTTC 2 cut(s) 1019, 1485
BstAPI GCANNNNNTGC 1 cut(s) 1158
BstC8I GCNNGC 2 cut(s) 141, 165
BstDEI CTNAG 7 cut(s) 193, 430, 452, 832, 870, 1383, 1428
BstDSI CCRYGG 1 cut(s) 762
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 4 cut(s) 556, 758, 1405, 1534
BstFNI CGCG 3 cut(s) 143, 1086, 1203
BstHHI GCGC 1 cut(s) 1088
BstKTI GATC 3 cut(s) 72, 741, 993
BstMAI GTCTC 3 cut(s) 202, 420, 455
BstMBI GATC 3 cut(s) 69, 738, 990
BstMWI GCNNNNNNNGC 7 cut(s) 173, 647, 755, 1007, 1158, 1583, 1592
BstNSI RCATGY 1 cut(s) 1520
BstSCI CCNGG 2 cut(s) 421, 1094
BstUI CGCG 3 cut(s) 143, 1086, 1203
BstV1I GCAGC 3 cut(s) 38, 736, 1388
BstV2I GAAGAC 2 cut(s) 690, 1644
BstX2I RGATCY 1 cut(s) 990
BstYI RGATCY 1 cut(s) 990
BsuRI GGCC 7 cut(s) 60, 75, 167, 899, 1010, 1022, 1595
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 1295
BtsCI GGATG 4 cut(s) 556, 758, 1405, 1534
BtsIMutI CAGTG 2 cut(s) 191, 1603
BveI ACCTGC 2 cut(s) 21, 459
Cac8I GCNNGC 2 cut(s) 141, 165
CaiI CAGNNNCTG 1 cut(s) 1382
CfoI GCGC 1 cut(s) 1088
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 7 cut(s) 109, 133, 166, 412, 471, 1009, 1020
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 2 cut(s) 586, 1290
CspAI ACCGGT 1 cut(s) 117
CviAII CATG 4 cut(s) 763, 941, 1517, 1568
CviQI GTAC 2 cut(s) 586, 1290
DdeI CTNAG 7 cut(s) 193, 430, 452, 832, 870, 1383, 1428
DpnI GATC 3 cut(s) 71, 740, 992
DpnII GATC 3 cut(s) 69, 738, 990
DrdI GACNNNNNNGTC 1 cut(s) 863
DseDI GACNNNNNNGTC 1 cut(s) 863
EaeI YGGCCR 2 cut(s) 73, 1593
Eam1104I CTCTTC 2 cut(s) 1019, 1485
EarI CTCTTC 2 cut(s) 1019, 1485
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 4 cut(s) 474, 762, 994, 1032
Eco24I GRGCYC 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 4 cut(s) 109, 133, 412, 471
Eco53kI GAGCTC 1 cut(s) 99
Eco57I CTGAAG 4 cut(s) 702, 971, 1000, 1326
EcoICRI GAGCTC 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoO109I RGGNCCY 4 cut(s) 166, 412, 1009, 1020
EcoRI GAATTC 1 cut(s) 556
EcoT14I CCWWGG 4 cut(s) 474, 762, 994, 1032
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 4 cut(s) 474, 762, 994, 1032
FaeI CATG 4 cut(s) 766, 944, 1520, 1571
FalI AAGNNNNNCTT 4 cut(s) 429, 461, 998, 1030
FatI CATG 4 cut(s) 762, 940, 1516, 1567
FauI CCCGC 1 cut(s) 937
Fnu4HI GCNGC 4 cut(s) 27, 144, 750, 1377
FokI GGATG 4 cut(s) 563, 745, 1412, 1541
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 27, 144, 750, 1377
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 1087
GluI GCNGC 4 cut(s) 27, 144, 750, 1377
GsaI CCCAGC 1 cut(s) 445
HaeIII GGCC 7 cut(s) 60, 75, 167, 899, 1010, 1022, 1595
HapII CCGG 4 cut(s) 33, 118, 423, 1096
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 1 cut(s) 1088
Hin1II CATG 4 cut(s) 766, 944, 1520, 1571
Hin6I GCGC 1 cut(s) 1086
HinP1I GCGC 1 cut(s) 1086
HincII GTYRAC 3 cut(s) 493, 502, 672
HindII GTYRAC 3 cut(s) 493, 502, 672
HindIII AAGCTT 1 cut(s) 1338
HinfI GANTC 4 cut(s) 514, 573, 1502, 1640
HpaI GTTAAC 1 cut(s) 493
HpaII CCGG 4 cut(s) 33, 118, 423, 1096
HphI GGTGA 5 cut(s) 143, 523, 622, 728, 1403
Hpy166II GTNNAC 5 cut(s) 10, 493, 502, 672, 1565
Hpy188III TCNNGA 4 cut(s) 124, 365, 602, 1223
Hpy8I GTNNAC 5 cut(s) 10, 493, 502, 672, 1565
HpyAV CCTTC 7 cut(s) 121, 197, 257, 984, 995, 1000, 1453
HpyCH4III ACNGT 4 cut(s) 461, 497, 852, 1333
HpyCH4IV ACGT 1 cut(s) 928
HpyCH4V TGCA 9 cut(s) 189, 650, 812, 887, 1127, 1166, 1247, 1376, 1520
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 647, 755, 1007, 1158, 1583, 1592
HpyF3I CTNAG 7 cut(s) 193, 430, 452, 832, 870, 1383, 1428
HpySE526I ACGT 1 cut(s) 928
Hsp92II CATG 4 cut(s) 766, 944, 1520, 1571
HspAI GCGC 1 cut(s) 1086
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 3 cut(s) 69, 738, 990
LmnI GCTCC 5 cut(s) 96, 104, 923, 1216, 1583
Lsp1109I GCAGC 3 cut(s) 38, 736, 1388
LweI GCATC 4 cut(s) 163, 767, 1097, 1411
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 928
MaeIII GTNAC 5 cut(s) 35, 328, 461, 865, 1327
MalI GATC 3 cut(s) 71, 740, 992
MboI GATC 3 cut(s) 69, 738, 990
MflI RGATCY 1 cut(s) 990
MhlI GDGCHC 2 cut(s) 101, 928
MlsI TGGCCA 1 cut(s) 1595
MluCI AATT 9 cut(s) 556, 605, 628, 698, 806, 892, 933, 1472, 1588
MluNI TGGCCA 1 cut(s) 1595
MlyI GAGTC 2 cut(s) 582, 1634
Mox20I TGGCCA 1 cut(s) 1595
MroXI GAANNNNTTC 2 cut(s) 119, 560
MscI TGGCCA 1 cut(s) 1595
MseI TTAA 9 cut(s) 180, 243, 486, 492, 701, 795, 905, 932, 1605
MslI CAYNNNNRTG 2 cut(s) 872, 1265
Msp20I TGGCCA 1 cut(s) 1595
MspI CCGG 4 cut(s) 33, 118, 423, 1096
MspR9I CCNGG 2 cut(s) 423, 1096
MvnI CGCG 3 cut(s) 143, 1086, 1203
MwoI GCNNNNNNNGC 7 cut(s) 173, 647, 755, 1007, 1158, 1583, 1592
NciI CCSGG 2 cut(s) 423, 1096
NcoI CCATGG 1 cut(s) 762
NdeII GATC 3 cut(s) 69, 738, 990
NlaIII CATG 4 cut(s) 766, 944, 1520, 1571
NlaIV GGNNCC 2 cut(s) 1000, 1585
NmeAIII GCCGAG 1 cut(s) 1027
NmuCI GTSAC 3 cut(s) 328, 865, 1327
NspI RCATGY 1 cut(s) 1520
PdmI GAANNNNTTC 2 cut(s) 119, 560
PfeI GAWTC 2 cut(s) 514, 1502
PinAI ACCGGT 1 cut(s) 117
PkrI GCNGC 4 cut(s) 28, 145, 751, 1378
PleI GAGTC 2 cut(s) 581, 1634
PpsI GAGTC 2 cut(s) 581, 1634
PpuMI RGGWCCY 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 412
PspFI CCCAGC 1 cut(s) 441
PspN4I GGNNCC 2 cut(s) 1000, 1585
PspPI GGNCC 7 cut(s) 109, 133, 166, 412, 471, 1009, 1020
PspPPI RGGWCCY 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 1382
PsuI RGATCY 1 cut(s) 990
RsaI GTAC 2 cut(s) 587, 1291
RsaNI GTAC 2 cut(s) 586, 1290
RseI CAYNNNNRTG 2 cut(s) 872, 1265
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 9 cut(s) 180, 243, 486, 492, 701, 795, 905, 932, 1605
SatI GCNGC 4 cut(s) 27, 144, 750, 1377
Sau3AI GATC 3 cut(s) 69, 738, 990
Sau96I GGNCC 7 cut(s) 109, 133, 166, 412, 471, 1009, 1020
ScaI AGTACT 1 cut(s) 1291
SchI GAGTC 2 cut(s) 582, 1634
ScrFI CCNGG 2 cut(s) 423, 1096
SduI GDGCHC 2 cut(s) 101, 928
SfaNI GCATC 4 cut(s) 163, 767, 1097, 1411
SinI GGWCC 4 cut(s) 109, 133, 412, 471
SmiMI CAYNNNNRTG 2 cut(s) 872, 1265
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 9 cut(s) 556, 605, 628, 698, 806, 892, 933, 1472, 1588
SsiI CCGC 5 cut(s) 5, 89, 143, 944, 1201
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 421, 1094
StyI CCWWGG 4 cut(s) 474, 762, 994, 1032
TaaI ACNGT 4 cut(s) 461, 497, 852, 1333
TaiI ACGT 1 cut(s) 931
TaqI TCGA 4 cut(s) 63, 171, 543, 693
TasI AATT 9 cut(s) 556, 605, 628, 698, 806, 892, 933, 1472, 1588
TatI WGTACW 1 cut(s) 1289
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 2 cut(s) 514, 1502
Tru1I TTAA 9 cut(s) 180, 243, 486, 492, 701, 795, 905, 932, 1605
Tru9I TTAA 9 cut(s) 180, 243, 486, 492, 701, 795, 905, 932, 1605
TscAI CASTG 2 cut(s) 191, 1603
TseFI GTSAC 3 cut(s) 328, 865, 1327
TseI GCWGC 3 cut(s) 26, 749, 1376
Tsp45I GTSAC 3 cut(s) 328, 865, 1327
TspDTI ATGAA 9 cut(s) 312, 380, 735, 839, 890, 1059, 1286, 1416, 1503
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 191, 1603
VpaK11BI GGWCC 4 cut(s) 109, 133, 412, 471
XagI CCTNNNNNAGG 1 cut(s) 545
XapI RAATTY 1 cut(s) 556
XceI RCATGY 1 cut(s) 1520
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XmnI GAANNNNTTC 2 cut(s) 119, 560
XspI CTAG 1 cut(s) 50
ZrmI AGTACT 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.