Rw7G009770

Nucleolar protein 56-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014044
Physical Location & Seq
Forward (+)
9068715 .. 9072124
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009770.1

Sequence Viewer

Length: 1500 bp
ATGGCGGTTTACCTGCTCTACGAAGCAGCTTCCGGTTACTCTCTGTTCCTAGCTCACGGCCTCGACCAGATCGGCCAGAACACCGAGGCGGTTCGGAGCTCCGTTTCGGACCTGAACCGGTTCGGGAAGGTGGTCCAGCTCGCGGCTTTTCACCCATTTGAGCAGGCCCTCGATGCCCTTAACCAGTGCAACTCAGTCTCCGAAGGGATTATGACTGATGAGTTGAGGAACTTTTTGGAACTTAATCTCCCAAAAGTAAAGGAAGGCAAGAAGCCAAAGTTCAGTTTGGGTGTATATGAACCCAAGCTCGGGTCTCACATATTTGAAGTGACTAAAATCCCCTGCCAAAGCAATGAGTTTGTTCTTGAGCTACTTCGTGGAGTTCGGCTTCATTTTGATAGGTTTATCAAGGATCTAAAGCCCGGGGACTTAGAGAAAGCCCAGCTTGGTCTCAGCCACAGTTACAGCAGGTCCAAGGTCAAGTTTAATGTTAACCGTGTTGACAATATGGTGATTCAAGCAATCTTCCTTTTAGATACCCTCGATAAGGATGTGAATTCTTTCTCAATGAGAGTCAGAGAATGGTACTCTTGGCATTTTCCTGAATTGGTGAAGATTGTCAATGACAATTATCTCTATGCTAAACTTGCAAAAACTATAAGAGATAAGTCAACTTTGTCTGAAGACAACCTCGAAGAATTAACTGATATTGTTGGGGATGAAGATAAGGCAAAGGAGATCATTGAAGCTGCCAAAGCATCCATGGTAGTTGGAGCACGATTAATTTCTCATGCGGGTAGTCTTACAAACTTGGCTAAATGCCCTTCTTCTACCCTTCAGATCCTTGGTGCCGAGAAGGCCCTGTTCAGGGCCTTGAAAACCAAGGGAAACACACCCAAGTATGGACTTATATTCCATTCATCTTTTATTGGTCGCGCATCTGCCCGGAACAAGGGCCGAATGGCTCGTTATCTTGCAAACAAGTGTTCTATTGCTTCTCGCATTGACTGCTTTGCAGATAATAGCACTACTGCTTTTGGGGAGAAACTCCGCGAACAAGTTGAGGAGCGTCTTGACTTTTATGACAAGGGTGTTGCACCTCGTAAAAACATTGATGTGATGAAATCTGCGATGGAGAGTACTCAAAGCAAAGATACAGATATGGAAACAGAAGAAGTGACTATTGAAGCTTCAGATAAGAAAAGCAAGAAGAAATCAAAGAGTGCAGCTACAGAGAATGGTGATGTGGATGAACCAGCTACAAATGGAGATGCTACTGAGGGTAAATCTGAAAAGAAGAAGAAGAAGAAGGACAAGAGGAAATTGGATGAGGAAGATGAAGAGATGGAGGATGCAAACAATGGACATGCTGAGGAGGATGGAACAAGCAAGAAGAAGAAGAAGAAGAAAAGTAAACATGGAGAGGCTGATGGAGCCGATTTGGCCAGTGATGTTAAGAAGAAGAAAAAGAAGAAATCTAAAAGTGAAGACTCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOP5NT Nop

Protein Analysis

499

Amino Acids

55.78

Weight (kDa)

8.57

Isoelectric Point (pI)

26.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOP5NT PF08156 4 - 69 7.9e-21 NOP5NT (NUC127) domain
Nop PF01798 177 - 254 3.9e-17 snoRNA binding domain, fibrillarin
Nop PF01798 256 - 359 1.9e-42 snoRNA binding domain, fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56110 AT3G12860
fragaria_vesca FvH4_5g07871
malus_domestica MD06G1145200.v1.1 MD14G1159900.v1.1
prunus_persica Prupe.5G153500_v2.0.a1 Prupe.5G153500_v2.0.a1
pyrus_communis pycom06g13450
rosa_chinensis RchiOBHm_Chr7g0190581
rosa_laevigata RLG00000004513
rosa_multiflora Rmu_sc0036974.1_g000001
rosa_roxburghii Rroxscaffold_3G00264490
rosa_rugosa Rorug06G0505400
rosa_samantha Rh7BG114800 Rh7CG116900 Rh7DG116000
rosa_wichuraiana Rw7G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 21, 459
AccB1I GGYRCC 1 cut(s) 848
AccII CGCG 3 cut(s) 143, 936, 1053
AciI CCGC 5 cut(s) 5, 89, 143, 794, 1051
AclWI GGATC 2 cut(s) 420, 835
AcoI YGGCCR 2 cut(s) 73, 1443
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 3 cut(s) 702, 821, 1176
AfaI GTAC 2 cut(s) 587, 1141
AfiI CCNNNNNNNGG 8 cut(s) 142, 308, 309, 547, 867, 868, 902, 952
AgeI ACCGGT 1 cut(s) 117
AgsI TTSAA 5 cut(s) 326, 518, 746, 877, 1187
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 2 cut(s) 101, 778
Alw26I GTCTC 3 cut(s) 202, 318, 455
AlwI GGATC 2 cut(s) 420, 835
Ama87I CYCGRG 2 cut(s) 308, 422
AoxI GGCC 7 cut(s) 58, 73, 165, 858, 870, 955, 1443
ApeKI GCWGC 3 cut(s) 26, 749, 1226
ApoI RAATTY 1 cut(s) 556
AseI ATTAAT 1 cut(s) 782
AsiGI ACCGGT 1 cut(s) 117
Asp700I GAANNNNTTC 2 cut(s) 119, 560
AspLEI GCGC 1 cut(s) 938
AspS9I GGNCC 7 cut(s) 109, 133, 166, 471, 859, 870, 955
AsuC2I CCSGG 3 cut(s) 423, 424, 946
AsuHPI GGTGA 4 cut(s) 143, 523, 622, 1253
AvaI CYCGRG 2 cut(s) 308, 422
AvaII GGWCC 3 cut(s) 109, 133, 471
BalI TGGCCA 1 cut(s) 1445
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 2 cut(s) 690, 1494
Bbv12I GWGCWC 2 cut(s) 101, 778
BbvCI CCTCAGC 1 cut(s) 1371
BbvI GCAGC 3 cut(s) 38, 736, 1238
BccI CCATC 4 cut(s) 1126, 1339, 1373, 1424
BceAI ACGGC 1 cut(s) 73
BcnI CCSGG 3 cut(s) 423, 424, 946
BcoDI GTCTC 3 cut(s) 202, 318, 455
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 1230
BfuAI ACCTGC 2 cut(s) 21, 459
BglI GCCNNNNNGGC 2 cut(s) 857, 1442
BisI GCNGC 4 cut(s) 27, 144, 750, 1227
BlsI GCNGC 4 cut(s) 28, 145, 751, 1228
BmcAI AGTACT 1 cut(s) 1141
Bme1390I CCNGG 3 cut(s) 423, 424, 946
Bme18I GGWCC 3 cut(s) 109, 133, 471
BmeT110I CYCGRG 2 cut(s) 308, 422
BmgT120I GGNCC 7 cut(s) 109, 133, 166, 471, 859, 870, 955
BmiI GGNNCC 2 cut(s) 850, 1435
BmrFI CCNGG 3 cut(s) 423, 424, 946
BmsI GCATC 5 cut(s) 163, 767, 947, 1261, 1342
BpiI GAAGAC 2 cut(s) 690, 1494
Bpu10I CCTNAGC 1 cut(s) 1371
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 3 cut(s) 423, 424, 946
BsaI GGTCTC 2 cut(s) 318, 455
BsaJI CCNNGG 7 cut(s) 84, 422, 423, 474, 762, 844, 882
BsaWI WCCGGW 2 cut(s) 32, 117
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 231, 261
Bsc4I CCNNNNNNNGG 8 cut(s) 142, 308, 309, 547, 867, 868, 902, 952
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 184, 1446
Bse3DI GCAATG 1 cut(s) 358
BseDI CCNNGG 7 cut(s) 84, 422, 423, 474, 762, 844, 882
BseGI GGATG 7 cut(s) 556, 724, 758, 1255, 1333, 1357, 1384
BseLI CCNNNNNNNGG 8 cut(s) 142, 308, 309, 547, 867, 868, 902, 952
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 4 cut(s) 207, 466, 1269, 1362
BseNI ACTGG 2 cut(s) 184, 1446
BseRI GAGGAG 2 cut(s) 1079, 1388
BseXI GCAGC 3 cut(s) 38, 736, 1238
BseYI CCCAGC 1 cut(s) 441
BsgI GTGCAG 1 cut(s) 1245
Bsh1236I CGCG 3 cut(s) 143, 936, 1053
BshFI GGCC 7 cut(s) 60, 75, 167, 860, 872, 957, 1445
BshNI GGYRCC 1 cut(s) 848
BshTI ACCGGT 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 101, 778
BsiHKCI CYCGRG 2 cut(s) 308, 422
BsiSI CCGG 4 cut(s) 33, 118, 423, 946
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 8 cut(s) 142, 308, 309, 547, 867, 868, 902, 952
BsmAI GTCTC 3 cut(s) 202, 318, 455
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 7 cut(s) 60, 75, 167, 860, 872, 957, 1445
Bso31I GGTCTC 2 cut(s) 318, 455
BsoBI CYCGRG 2 cut(s) 308, 422
Bsp1286I GDGCHC 2 cut(s) 101, 778
Bsp143I GATC 4 cut(s) 69, 412, 738, 840
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 5 cut(s) 5, 89, 143, 794, 1051
BspANI GGCC 7 cut(s) 60, 75, 167, 860, 872, 957, 1445
BspCNI CTCAG 4 cut(s) 206, 465, 1270, 1363
BspFNI CGCG 3 cut(s) 143, 936, 1053
BspLI GGNNCC 2 cut(s) 850, 1435
BspMI ACCTGC 2 cut(s) 21, 459
BspPI GGATC 2 cut(s) 420, 835
BspT107I GGYRCC 1 cut(s) 848
BspTNI GGTCTC 2 cut(s) 318, 455
BsrDI GCAATG 1 cut(s) 358
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 2 cut(s) 184, 1446
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 7 cut(s) 84, 422, 423, 474, 762, 844, 882
BssMI GATC 4 cut(s) 69, 412, 738, 840
BssT1I CCWWGG 4 cut(s) 474, 762, 844, 882
Bst4CI ACNGT 2 cut(s) 461, 497
Bst6I CTCTTC 1 cut(s) 1335
BstAPI GCANNNNNTGC 1 cut(s) 1008
BstC8I GCNNGC 2 cut(s) 141, 165
BstDEI CTNAG 5 cut(s) 193, 430, 452, 1278, 1371
BstDSI CCRYGG 1 cut(s) 762
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 7 cut(s) 556, 724, 758, 1255, 1333, 1357, 1384
BstFNI CGCG 3 cut(s) 143, 936, 1053
BstHHI GCGC 1 cut(s) 938
BstKTI GATC 4 cut(s) 72, 415, 741, 843
BstMAI GTCTC 3 cut(s) 202, 318, 455
BstMBI GATC 4 cut(s) 69, 412, 738, 840
BstMWI GCNNNNNNNGC 7 cut(s) 173, 647, 755, 857, 1008, 1433, 1442
BstNSI RCATGY 1 cut(s) 1370
BstSCI CCNGG 3 cut(s) 421, 422, 944
BstSFI CTRYAG 1 cut(s) 1230
BstUI CGCG 3 cut(s) 143, 936, 1053
BstV1I GCAGC 3 cut(s) 38, 736, 1238
BstV2I GAAGAC 2 cut(s) 690, 1494
BstX2I RGATCY 2 cut(s) 412, 840
BstYI RGATCY 2 cut(s) 412, 840
BsuRI GGCC 7 cut(s) 60, 75, 167, 860, 872, 957, 1445
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 1145
BtsCI GGATG 7 cut(s) 556, 724, 758, 1255, 1333, 1357, 1384
BtsIMutI CAGTG 2 cut(s) 191, 1453
BveI ACCTGC 2 cut(s) 21, 459
Cac8I GCNNGC 2 cut(s) 141, 165
CfoI GCGC 1 cut(s) 938
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 7 cut(s) 109, 133, 166, 471, 859, 870, 955
Cfr9I CCCGGG 1 cut(s) 422
CseI GACGC 1 cut(s) 1058
Csp6I GTAC 2 cut(s) 586, 1140
CspAI ACCGGT 1 cut(s) 117
CviAII CATG 4 cut(s) 763, 791, 1367, 1418
CviQI GTAC 2 cut(s) 586, 1140
DdeI CTNAG 5 cut(s) 193, 430, 452, 1278, 1371
DpnI GATC 4 cut(s) 71, 414, 740, 842
DpnII GATC 4 cut(s) 69, 412, 738, 840
EaeI YGGCCR 2 cut(s) 73, 1443
Eam1104I CTCTTC 1 cut(s) 1335
EarI CTCTTC 1 cut(s) 1335
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 4 cut(s) 474, 762, 844, 882
Eco24I GRGCYC 1 cut(s) 101
Eco31I GGTCTC 2 cut(s) 318, 455
Eco47I GGWCC 3 cut(s) 109, 133, 471
Eco53kI GAGCTC 1 cut(s) 99
Eco57I CTGAAG 3 cut(s) 702, 821, 1176
Eco88I CYCGRG 2 cut(s) 308, 422
EcoICRI GAGCTC 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoO109I RGGNCCY 3 cut(s) 166, 859, 870
EcoRI GAATTC 1 cut(s) 556
EcoT14I CCWWGG 4 cut(s) 474, 762, 844, 882
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 4 cut(s) 474, 762, 844, 882
FaeI CATG 4 cut(s) 766, 794, 1370, 1421
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FaqI GGGAC 1 cut(s) 440
FatI CATG 4 cut(s) 762, 790, 1366, 1417
FauI CCCGC 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 27, 144, 750, 1227
FokI GGATG 7 cut(s) 563, 731, 745, 1262, 1340, 1364, 1391
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 27, 144, 750, 1227
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 937
GluI GCNGC 4 cut(s) 27, 144, 750, 1227
GsaI CCCAGC 1 cut(s) 445
HaeIII GGCC 7 cut(s) 60, 75, 167, 860, 872, 957, 1445
HapII CCGG 4 cut(s) 33, 118, 423, 946
HgaI GACGC 1 cut(s) 1058
HhaI GCGC 1 cut(s) 938
Hin1II CATG 4 cut(s) 766, 794, 1370, 1421
Hin6I GCGC 1 cut(s) 936
HinP1I GCGC 1 cut(s) 936
HincII GTYRAC 3 cut(s) 493, 502, 672
HindII GTYRAC 3 cut(s) 493, 502, 672
HindIII AAGCTT 1 cut(s) 1188
HinfI GANTC 3 cut(s) 514, 573, 1490
HpaI GTTAAC 1 cut(s) 493
HpaII CCGG 4 cut(s) 33, 118, 423, 946
HphI GGTGA 4 cut(s) 143, 523, 622, 1253
Hpy166II GTNNAC 5 cut(s) 10, 493, 502, 672, 1415
Hpy188I TCNGA 9 cut(s) 96, 109, 202, 578, 682, 840, 1195, 1291, 1495
Hpy188III TCNNGA 4 cut(s) 124, 365, 602, 1073
Hpy8I GTNNAC 5 cut(s) 10, 493, 502, 672, 1415
HpyAV CCTTC 7 cut(s) 121, 197, 257, 834, 845, 850, 1303
HpyCH4III ACNGT 2 cut(s) 461, 497
HpyCH4V TGCA 7 cut(s) 189, 650, 977, 1016, 1097, 1226, 1355
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 647, 755, 857, 1008, 1433, 1442
HpyF3I CTNAG 5 cut(s) 193, 430, 452, 1278, 1371
Hsp92II CATG 4 cut(s) 766, 794, 1370, 1421
HspAI GCGC 1 cut(s) 936
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 4 cut(s) 69, 412, 738, 840
LmnI GCTCC 5 cut(s) 96, 104, 773, 1066, 1433
Lsp1109I GCAGC 3 cut(s) 38, 736, 1238
LweI GCATC 5 cut(s) 163, 767, 947, 1261, 1342
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 4 cut(s) 35, 328, 461, 1177
MalI GATC 4 cut(s) 71, 414, 740, 842
MboI GATC 4 cut(s) 69, 412, 738, 840
MflI RGATCY 2 cut(s) 412, 840
MhlI GDGCHC 2 cut(s) 101, 778
MlsI TGGCCA 1 cut(s) 1445
MluCI AATT 6 cut(s) 556, 605, 628, 698, 783, 1322
MluNI TGGCCA 1 cut(s) 1445
MlyI GAGTC 2 cut(s) 582, 1484
MmeI TCCRAC 1 cut(s) 751
Mox20I TGGCCA 1 cut(s) 1445
MroXI GAANNNNTTC 2 cut(s) 119, 560
MscI TGGCCA 1 cut(s) 1445
MseI TTAA 7 cut(s) 180, 243, 486, 492, 701, 782, 1455
MslI CAYNNNNRTG 1 cut(s) 1115
Msp20I TGGCCA 1 cut(s) 1445
MspI CCGG 4 cut(s) 33, 118, 423, 946
MspR9I CCNGG 3 cut(s) 423, 424, 946
MvnI CGCG 3 cut(s) 143, 936, 1053
MwoI GCNNNNNNNGC 7 cut(s) 173, 647, 755, 857, 1008, 1433, 1442
NciI CCSGG 3 cut(s) 423, 424, 946
NcoI CCATGG 1 cut(s) 762
NdeII GATC 4 cut(s) 69, 412, 738, 840
NlaIII CATG 4 cut(s) 766, 794, 1370, 1421
NlaIV GGNNCC 2 cut(s) 850, 1435
NmeAIII GCCGAG 1 cut(s) 877
NmuCI GTSAC 2 cut(s) 328, 1177
NspI RCATGY 1 cut(s) 1370
PdmI GAANNNNTTC 2 cut(s) 119, 560
PfeI GAWTC 1 cut(s) 514
PinAI ACCGGT 1 cut(s) 117
PkrI GCNGC 4 cut(s) 28, 145, 751, 1228
PleI GAGTC 2 cut(s) 581, 1484
PpsI GAGTC 2 cut(s) 581, 1484
PshBI ATTAAT 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 101
PspFI CCCAGC 1 cut(s) 441
PspN4I GGNNCC 2 cut(s) 850, 1435
PspPI GGNCC 7 cut(s) 109, 133, 166, 471, 859, 870, 955
PsuI RGATCY 2 cut(s) 412, 840
RsaI GTAC 2 cut(s) 587, 1141
RsaNI GTAC 2 cut(s) 586, 1140
RseI CAYNNNNRTG 1 cut(s) 1115
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 7 cut(s) 180, 243, 486, 492, 701, 782, 1455
SatI GCNGC 4 cut(s) 27, 144, 750, 1227
Sau3AI GATC 4 cut(s) 69, 412, 738, 840
Sau96I GGNCC 7 cut(s) 109, 133, 166, 471, 859, 870, 955
ScaI AGTACT 1 cut(s) 1141
SchI GAGTC 2 cut(s) 582, 1484
ScrFI CCNGG 3 cut(s) 423, 424, 946
SduI GDGCHC 2 cut(s) 101, 778
SfaNI GCATC 5 cut(s) 163, 767, 947, 1261, 1342
SfcI CTRYAG 1 cut(s) 1230
SinI GGWCC 3 cut(s) 109, 133, 471
SmaI CCCGGG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 1115
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 6 cut(s) 556, 605, 628, 698, 783, 1322
SsiI CCGC 5 cut(s) 5, 89, 143, 794, 1051
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 3 cut(s) 421, 422, 944
StyI CCWWGG 4 cut(s) 474, 762, 844, 882
TaaI ACNGT 2 cut(s) 461, 497
TaqI TCGA 4 cut(s) 63, 171, 543, 693
TasI AATT 6 cut(s) 556, 605, 628, 698, 783, 1322
TatI WGTACW 1 cut(s) 1139
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 7 cut(s) 180, 243, 486, 492, 701, 782, 1455
Tru9I TTAA 7 cut(s) 180, 243, 486, 492, 701, 782, 1455
TscAI CASTG 2 cut(s) 191, 1453
TseFI GTSAC 2 cut(s) 328, 1177
TseI GCWGC 3 cut(s) 26, 749, 1226
Tsp45I GTSAC 2 cut(s) 328, 1177
TspDTI ATGAA 7 cut(s) 312, 380, 735, 909, 1136, 1266, 1353
TspGWI ACGGA 1 cut(s) 91
TspMI CCCGGG 1 cut(s) 422
TspRI CASTG 2 cut(s) 191, 1453
VpaK11BI GGWCC 3 cut(s) 109, 133, 471
VspI ATTAAT 1 cut(s) 782
XagI CCTNNNNNAGG 1 cut(s) 545
XapI RAATTY 1 cut(s) 556
XceI RCATGY 1 cut(s) 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XmaI CCCGGG 1 cut(s) 422
XmnI GAANNNNTTC 2 cut(s) 119, 560
XspI CTAG 1 cut(s) 50
ZrmI AGTACT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.