Rroxscaffold_4G00285020

two-component response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014474
Physical Location & Seq
Reverse (-)
6486709 .. 6488076
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285020.1

Sequence Viewer

Length: 450 bp
ATGGAGTCTGGATTTTCTTCTTCTTCAATGGGTTTCATGGAAAATGTGGAGCAACCTCATGTTTTGGCTGTGGATGACAGTGATATTGATCGGAAACTTATCGAAAAGCTGCTCAAGAATTTCTCTTGCAAAGTGACTACTGCAGAAAATGGGGCAAGAGCATTGGAGTTTCTAGGCTTGGGAGATCATCCAAACCATTGCAATATCTCAAAGGTAAATCTGGTAATTACAGATTATTGTATGCCAGGAATGACAGGATATGAACTGCTTAAGAAAATCAAGGAATCATCAGTCATGAAGGAGGTTCCAGTGGTGATTATGTCATCTGAAAATATACCGAATCGGATTAGCAAGTGCTTGGAGGAAGGAGCTCATATGTTCATGCTAAAACCGATCAAACAGTCGGACATTAAGCAATTGAGATGTCATCTGATGAATTGCAGGAGCTGA

Protein Analysis

149

Amino Acids

16.72

Weight (kDa)

6.51

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 21 - 139 5.4e-22 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56380 AT3G56380
fragaria_vesca FvH4_7g25970
malus_domestica MD01G1168100.v1.1 MD07G1234100.v1.1
prunus_persica Prupe.2G264500_v2.0.a1
pyrus_communis pycom01g18110 pycom07g21190
rosa_chinensis RchiOBHm_Chr1g0372351
rosa_laevigata RLG00000026905
rosa_roxburghii Rroxscaffold_4G00285020
rosa_rugosa Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379800 Rh1BG343300 Rh1CG356800
rosa_wichuraiana Rw1G033390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 118
AflII CTTAAG 1 cut(s) 269
AgsI TTSAA 1 cut(s) 27
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 3 cut(s) 109, 371, 447
AluI AGCT 3 cut(s) 109, 371, 447
Alw21I GWGCWC 1 cut(s) 373
AlwNI CAGNNNCTG 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 325
BanII GRGCYC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 373
BbvI GCAGC 1 cut(s) 96
BciT130I CCWGG 1 cut(s) 246
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 141
BfrI CTTAAG 1 cut(s) 269
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
Bme1390I CCNGG 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 196
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 1 cut(s) 246
BseGI GGATG 2 cut(s) 79, 187
BseJI GATNNNNATC 1 cut(s) 87
BseMI GCAATG 1 cut(s) 196
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 373
Bsp143I GATC 3 cut(s) 88, 184, 393
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 145
BspTI CTTAAG 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 196
BsrI ACTGG 1 cut(s) 308
BssMI GATC 3 cut(s) 88, 184, 393
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 80, 402
BstAFI CTTAAG 1 cut(s) 269
BstF5I GGATG 2 cut(s) 79, 187
BstKTI GATC 3 cut(s) 91, 187, 396
BstMBI GATC 3 cut(s) 88, 184, 393
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 96
BtsCI GGATG 2 cut(s) 79, 187
BtsIMutI CAGTG 2 cut(s) 85, 315
CaiI CAGNNNCTG 1 cut(s) 447
CciI TCATGA 1 cut(s) 294
CviAII CATG 4 cut(s) 37, 59, 295, 382
CviJI RGCY 5 cut(s) 68, 109, 177, 371, 447
CviKI_1 RGCY 5 cut(s) 68, 109, 177, 371, 447
DpnI GATC 3 cut(s) 90, 186, 395
DpnII GATC 3 cut(s) 88, 184, 393
Ecl136II GAGCTC 1 cut(s) 371
Eco24I GRGCYC 1 cut(s) 373
Eco53kI GAGCTC 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 373
FaeI CATG 4 cut(s) 40, 62, 298, 385
FatI CATG 4 cut(s) 36, 58, 294, 381
FauNDI CATATG 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 2 cut(s) 86, 174
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 1 cut(s) 173
GluI GCNGC 1 cut(s) 110
Hin1II CATG 4 cut(s) 40, 62, 298, 385
HinfI GANTC 3 cut(s) 5, 284, 340
HphI GGTGA 1 cut(s) 325
Hpy188I TCNGA 5 cut(s) 93, 328, 345, 406, 432
Hpy188III TCNNGA 3 cut(s) 9, 115, 295
HpyAV CCTTC 2 cut(s) 292, 359
HpyCH4III ACNGT 2 cut(s) 80, 402
HpyCH4V TGCA 4 cut(s) 129, 143, 201, 441
Hsp92II CATG 4 cut(s) 40, 62, 298, 385
Kzo9I GATC 3 cut(s) 88, 184, 393
LmnI GCTCC 3 cut(s) 49, 368, 444
LpnPI CCDG 6 cut(s) 206, 231, 240, 258, 321, 427
Lsp1109I GCAGC 1 cut(s) 96
MaeI CTAG 1 cut(s) 173
MaeIII GTNAC 1 cut(s) 133
MalI GATC 3 cut(s) 90, 186, 395
MboI GATC 3 cut(s) 88, 184, 393
MboII GAAGA 3 cut(s) 9, 12, 15
MfeI CAATTG 1 cut(s) 416
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 4 cut(s) 118, 225, 416, 436
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 3 cut(s) 66, 295, 355
MseI TTAA 2 cut(s) 270, 411
MspCI CTTAAG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 375
NdeII GATC 3 cut(s) 88, 184, 393
NlaIII CATG 4 cut(s) 40, 62, 298, 385
NlaIV GGNNCC 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 133
PagI TCATGA 1 cut(s) 294
PfeI GAWTC 2 cut(s) 284, 340
PkrI GCNGC 1 cut(s) 111
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 306
PstI CTGCAG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 447
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 270, 411
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 3 cut(s) 88, 184, 393
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 1 cut(s) 373
SetI ASST 6 cut(s) 58, 111, 216, 306, 373, 449
SfcI CTRYAG 1 cut(s) 141
SmlI CTYRAG 2 cut(s) 113, 269
SmoI CTYRAG 2 cut(s) 113, 269
Sse9I AATT 4 cut(s) 118, 225, 416, 436
SspMI CTAG 1 cut(s) 173
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 80, 402
TaqI TCGA 1 cut(s) 102
TasI AATT 4 cut(s) 118, 225, 416, 436
TfiI GAWTC 2 cut(s) 284, 340
Tru1I TTAA 2 cut(s) 270, 411
Tru9I TTAA 2 cut(s) 270, 411
TscAI CASTG 2 cut(s) 85, 315
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 5 cut(s) 25, 276, 311, 370, 449
TspRI CASTG 2 cut(s) 85, 315
Vha464I CTTAAG 1 cut(s) 269
XapI RAATTY 1 cut(s) 118
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.