Rroxscaffold_4G00294570

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017363
Physical Location & Seq
Forward (+)
14617526 .. 14619438
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294570.1

Sequence Viewer

Length: 1851 bp
ATGCGTACTCTGTTGAAACCACTACTAGCACAAAACCCATGTTGCATCTTAGACTATGCACCAATTTTCCAATCCTTAACTGGGCAAAACTTGTTGAAGCTAGGCCAACAGGTCCACACCCATATGGTCCTGCGTGGCCTCGAACCCAATGCCTATCTTGGCGCCAAGATGGTTGCAATGTACGCCAGCTCTGGTGATCTCCACTCAGCTGTTAATGTCTTTCACAGAGTAAAATTCCCATCTCCACTTTTGTACAATTCGATTATTCGGGCTTATACCCTTCATGGTTACTCTGTGAGAACTATGGAAATTTATGGTCAGATGCATTGTTTAGGCCTAAAGGGTGATCATTTTACCTACCCTTTTGTGCTCAAGTGTTGTGCAGAGTTGTCGAATATTTGGATGGGGAAATGTGTTCATGGGCTGAGCTTGAGAACTGGGTTGGAGTCTGATATGTATGTTGGGACTTCTTTGATTGACATGTATGTGAAATGTTGTGAGATGGGCAATGCACGTAAGCTGTTCGATGAAATGTCTGTGAGAGACGTTTCGTCGTGGAATGCGTTGATTGCGGGCTACATGAGAGAGGGTGGGATTTGTTCTGCTGAGGAGTTGTTTGGACAAATGAGGTGCAAGAATATTGTGTCTTGGACTGCTATGATATCAGGGTATGCGCAGAATGGGCTGGCTGAGCAGGCATTGAGTGTGTTTGATGATATGTTGAAGAATGATTCGGAAGTTAAGCCGAATTGGGTGACGGTAATGAGTGTTCTTCCTGCATGTGCACATTCGGCTGCGCTAGAGCGGGGAGGAAGATTCACAAGTTTGCTAGCGGGATTGACCGCGCTGGTAGCAATGTATGCTAAATGTGGGAGCCTTTCTGATGCTCGTCAGTGTTTTGATAGGATTCGTGGAAGTGAGAAGAATTTGGTTGTATGGAATACTATGATCACTGCATATGCGTCCCATGCATGCGGATCAGAAGCTGTGTCCACTTTTGAGGACATGGTGAGAGCCGGTGTTCAACCTGATCATATAACATTCACGGGATTGTTATCTGGATGCAGTCATTCAGGGCTTGTTGATGTTGGCTTGAAATACTTCGATGGCATGAAGACAGTATACTCTGTTGAACCAAGAGTTGAACATTATGCTTGTGTTGTTGATCTATTGGGTCGTGCAGGGCGATTGATGGAAGCAGTGGATGTCGTAGATAAAATGCCAATGCAAGCAGGGCCAAGCATTTGGGGTGCACTGTTATCAGCTTGTCGAAACCACCCAAACTTGGAAATTGCCGAAACTGCAGCCAGAAATCTCTTCATTTTGGAACCAGACAACAGTGGCAACTATGTCCTGCTATCTAATATATATGCTGAAGCTGGCATGTGGAAGGAGGTGGACAAATTGAGAATTCTATTAAAATCTCAAGGGATGAAGAAGAGCCCTGGAGGCAGTTGGATAGAGGTCAATGGAAAAGCTCATCTGTTTCTTGGTGGAGATACATCTCACTCTCAAGCAAATGAAATTTACATGTTACTGGAGGAGTTACCGAAAAGGATGAAGGCAGCTGGTTATGTACCTGACACTAGGTTTGCTTTGCATGATGTTAGTGAAGAGGAGAAAGAACATAACCTTACAACCCACAGTGAAAAGCTTGCCATTGCCTTCGGACTTCTTAATACTGATCCTGGAGAAGTTCTTCGTGTGACAAAGAACCTTAGAATTTGTGGTGATTGTCATACAGCCACCAAGTTCATATCTCGAATCTATGGGAGGGAGATTATCGTAAGAGACGTGAATAGATTCCATCACTTTAAAGACGGGCACTGTTCATGTGGAGATTATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

616

Amino Acids

68.23

Weight (kDa)

6.76

Isoelectric Point (pI)

36.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 212 - 262 7.7e-10 PPR repeat family
PPR PF01535 215 - 243 1.2e-08 PPR repeat
PPR_2 PF13041 309 - 356 1.3e-12 PPR repeat family
E_motif PF20431 426 - 488 7.4e-25 E motif
DYW_deaminase PF14432 524 - 616 9.8e-45 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017363)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18340
malus_domestica MD07G1165900.v1.1
prunus_persica Prupe.2G205400_v2.0.a1
pyrus_communis pycom07g16150
rosa_chinensis RchiOBHm_Chr1g0362051
rosa_laevigata RLG00000002139 RLG00000027677
rosa_multiflora Rmu_sc0010614.1_g000005 Rmu_sc0020775.1_g000001
rosa_roxburghii Rroxscaffold_4G00294570
rosa_rugosa Rorug01G0293100
rosa_samantha Rh1BG266600
rosa_wichuraiana Rw1G027010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 675
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 805
AccI GTMKAC 1 cut(s) 1122
AccII CGCG 1 cut(s) 845
AciI CCGC 5 cut(s) 572, 805, 833, 843, 975
AclWI GGATC 2 cut(s) 985, 1679
AcsI RAATTY 6 cut(s) 233, 309, 925, 1410, 1524, 1722
AcuI CTGAAG 1 cut(s) 1395
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 4 cut(s) 7, 182, 254, 1578
AfiI CCNNNNNNNGG 2 cut(s) 81, 1285
AflIII ACRYGT 2 cut(s) 480, 1530
AgsI TTSAA 7 cut(s) 16, 97, 724, 1025, 1096, 1133, 1145
AhdI GACNNNNNGTC 1 cut(s) 550
AjiI CACGTC 1 cut(s) 1795
AjnI CCWGG 2 cut(s) 1444, 1687
Alw21I GWGCWC 3 cut(s) 372, 787, 1255
Alw26I GTCTC 2 cut(s) 537, 1785
Alw44I GTGCAC 2 cut(s) 783, 1251
AlwI GGATC 2 cut(s) 985, 1679
AlwNI CAGNNNCTG 1 cut(s) 986
AoxI GGCC 4 cut(s) 103, 136, 334, 1235
ApaLI GTGCAC 2 cut(s) 783, 1251
ApeKI GCWGC 3 cut(s) 794, 1304, 1565
ApoI RAATTY 6 cut(s) 233, 309, 925, 1410, 1524, 1722
ArsI GACNNNNNNTTYG 2 cut(s) 1574, 1606
Asp700I GAANNNNTTC 3 cut(s) 1100, 1698, 1802
AspLEI GCGC 4 cut(s) 164, 676, 799, 847
AspS9I GGNCC 3 cut(s) 112, 127, 1235
AsuHPI GGTGA 5 cut(s) 206, 356, 766, 1021, 1742
AsuNHI GCTAGC 1 cut(s) 829
AvaII GGWCC 2 cut(s) 112, 127
BaeGI GKGCMC 3 cut(s) 787, 1255, 1827
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 1445
BarI GAAGNNNNNNTAC 2 cut(s) 1106, 1138
BbsI GAAGAC 1 cut(s) 1121
Bbv12I GWGCWC 3 cut(s) 372, 787, 1255
BbvCI CCTCAGC 1 cut(s) 606
BbvI GCAGC 3 cut(s) 781, 1316, 1577
BccI CCATC 7 cut(s) 163, 247, 397, 496, 1100, 1186, 1815
BcgI CGANNNNNNTGC 4 cut(s) 131, 165, 372, 406
BciT130I CCWGG 2 cut(s) 1446, 1689
BclI TGATCA 3 cut(s) 346, 948, 1030
BcoDI GTCTC 2 cut(s) 537, 1785
BfaI CTAG 5 cut(s) 26, 101, 800, 830, 1587
BfmI CTRYAG 1 cut(s) 1302
BfoI RGCGCY 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 1449
BisI GCNGC 3 cut(s) 795, 1305, 1566
BlpI GCTNAGC 2 cut(s) 425, 690
BlsI GCNGC 3 cut(s) 796, 1306, 1567
Bme1390I CCNGG 2 cut(s) 1446, 1689
Bme18I GGWCC 2 cut(s) 112, 127
BmeRI GACNNNNNGTC 1 cut(s) 550
BmgBI CACGTC 1 cut(s) 1795
BmgT120I GGNCC 3 cut(s) 112, 127, 1235
BmiI GGNNCC 3 cut(s) 163, 875, 1329
BmrFI CCNGG 2 cut(s) 1446, 1689
BmrI ACTGGG 2 cut(s) 90, 447
BmsI GCATC 4 cut(s) 54, 312, 874, 1052
BmtI GCTAGC 1 cut(s) 833
BmuI ACTGGG 2 cut(s) 90, 447
BpiI GAAGAC 1 cut(s) 1121
BpmI CTGGAG 3 cut(s) 1467, 1559, 1710
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 2 cut(s) 425, 690
BpuEI CTTGAG 4 cut(s) 356, 451, 1410, 1497
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 1054
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 1444
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 1285
Bse118I RCCGGY 1 cut(s) 1016
Bse1I ACTGG 3 cut(s) 85, 442, 1542
Bse3DI GCAATG 4 cut(s) 183, 514, 861, 1659
Bse8I GATNNNNATC 1 cut(s) 1054
BseBI CCWGG 2 cut(s) 1446, 1689
BseDI CCNNGG 1 cut(s) 1444
BseGI GGATG 5 cut(s) 408, 1067, 1210, 1437, 1563
BseJI GATNNNNATC 1 cut(s) 1054
BseLI CCNNNNNNNGG 2 cut(s) 81, 1285
BseMI GCAATG 4 cut(s) 183, 514, 861, 1659
BseMII CTCAG 4 cut(s) 219, 416, 597, 681
BseNI ACTGG 3 cut(s) 85, 442, 1542
BseRI GAGGAG 3 cut(s) 623, 1556, 1631
BseSI GKGCMC 3 cut(s) 787, 1255, 1827
BseXI GCAGC 3 cut(s) 781, 1316, 1577
BsgI GTGCAG 2 cut(s) 402, 1200
Bsh1236I CGCG 1 cut(s) 845
BshFI GGCC 4 cut(s) 105, 138, 336, 1237
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 3 cut(s) 372, 787, 1255
BsiSI CCGG 1 cut(s) 1017
BslFI GGGAC 2 cut(s) 478, 949
BslI CCNNNNNNNGG 2 cut(s) 81, 1285
BsmAI GTCTC 2 cut(s) 537, 1785
BsmBI CGTCTC 2 cut(s) 537, 1785
BsmFI GGGAC 2 cut(s) 478, 949
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 4 cut(s) 105, 138, 336, 1237
Bsp1286I GDGCHC 5 cut(s) 372, 787, 1255, 1445, 1827
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
Bsp1720I GCTNAGC 2 cut(s) 425, 690
BspACI CCGC 5 cut(s) 572, 805, 833, 843, 975
BspANI GGCC 4 cut(s) 105, 138, 336, 1237
BspCNI CTCAG 4 cut(s) 218, 417, 598, 682
BspFNI CGCG 1 cut(s) 845
BspLI GGNNCC 3 cut(s) 163, 875, 1329
BspMAI CTGCAG 1 cut(s) 1306
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 2 cut(s) 985, 1679
BspQI GCTCTTC 1 cut(s) 1433
BspT107I GGYRCC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 805
BsrDI GCAATG 4 cut(s) 183, 514, 861, 1659
BsrFI RCCGGY 1 cut(s) 1016
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 3 cut(s) 85, 442, 1542
BssAI RCCGGY 1 cut(s) 1016
BssECI CCNNGG 1 cut(s) 1444
BssMI GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
BssNAI GTATAC 1 cut(s) 1123
BssNI GRCGYC 1 cut(s) 162
Bst1107I GTATAC 1 cut(s) 1123
Bst2UI CCWGG 2 cut(s) 1446, 1689
Bst4CI ACNGT 6 cut(s) 760, 1120, 1257, 1340, 1646, 1829
Bst6I CTCTTC 3 cut(s) 1322, 1433, 1608
BstACI GRCGYC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 860
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 9 cut(s) 187, 574, 687, 696, 831, 973, 1230, 1381, 1656
BstDEI CTNAG 6 cut(s) 49, 205, 425, 606, 690, 1718
BstF5I GGATG 5 cut(s) 408, 1067, 1210, 1437, 1563
BstFNI CGCG 1 cut(s) 845
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 164, 676, 799, 847
BstKTI GATC 7 cut(s) 199, 349, 951, 980, 1033, 1168, 1687
BstMAI GTCTC 2 cut(s) 537, 1785
BstMBI GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
BstNI CCWGG 2 cut(s) 1446, 1689
BstNSI RCATGY 5 cut(s) 484, 783, 975, 1387, 1534
BstSCI CCNGG 2 cut(s) 1444, 1687
BstSFI CTRYAG 1 cut(s) 1302
BstSLI GKGCMC 3 cut(s) 787, 1255, 1827
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 3 cut(s) 781, 1316, 1577
BstV2I GAAGAC 1 cut(s) 1121
BstXI CCANNNNNNTGG 1 cut(s) 1245
BstZ17I GTATAC 1 cut(s) 1123
BsuRI GGCC 4 cut(s) 105, 138, 336, 1237
BtrI CACGTC 1 cut(s) 1795
BtsCI GGATG 5 cut(s) 408, 1067, 1210, 1437, 1563
BtsI GCAGTG 2 cut(s) 951, 1206
BtsIMutI CAGTG 7 cut(s) 899, 951, 1206, 1253, 1345, 1651, 1825
Cac8I GCNNGC 9 cut(s) 187, 574, 687, 696, 831, 973, 1230, 1381, 1656
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 4 cut(s) 164, 676, 799, 847
Cfr10I RCCGGY 1 cut(s) 1016
Cfr13I GGNCC 3 cut(s) 112, 127, 1235
CseI GACGC 1 cut(s) 951
Csp6I GTAC 4 cut(s) 6, 181, 253, 1577
CviQI GTAC 4 cut(s) 6, 181, 253, 1577
DdeI CTNAG 6 cut(s) 49, 205, 425, 606, 690, 1718
DinI GGCGCC 1 cut(s) 163
DpnI GATC 7 cut(s) 198, 348, 950, 979, 1032, 1167, 1686
DpnII GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
DraI TTTAAA 1 cut(s) 1816
DriI GACNNNNNGTC 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 1322, 1433, 1608
Eam1105I GACNNNNNGTC 1 cut(s) 550
EarI CTCTTC 3 cut(s) 1322, 1433, 1608
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 1445
Eco32I GATATC 1 cut(s) 663
Eco47I GGWCC 2 cut(s) 112, 127
Eco57I CTGAAG 1 cut(s) 1395
EcoRI GAATTC 1 cut(s) 1410
EcoRII CCWGG 2 cut(s) 1444, 1687
EcoRV GATATC 1 cut(s) 663
EcoT22I ATGCAT 2 cut(s) 327, 973
EcoT38I GRGCYC 1 cut(s) 1445
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
Esp3I CGTCTC 2 cut(s) 537, 1785
FaqI GGGAC 2 cut(s) 478, 949
FauI CCCGC 3 cut(s) 565, 798, 826
FauNDI CATATG 2 cut(s) 123, 958
FbaI TGATCA 3 cut(s) 346, 948, 1030
FblI GTMKAC 1 cut(s) 1122
Fnu4HI GCNGC 3 cut(s) 795, 1305, 1566
FokI GGATG 5 cut(s) 415, 1074, 1217, 1444, 1570
FriOI GRGCYC 1 cut(s) 1445
Fsp4HI GCNGC 3 cut(s) 795, 1305, 1566
FspBI CTAG 5 cut(s) 26, 101, 800, 830, 1587
FspI TGCGCA 1 cut(s) 675
GlaI GCGC 4 cut(s) 163, 675, 798, 846
GluI GCNGC 3 cut(s) 795, 1305, 1566
GsuI CTGGAG 3 cut(s) 1467, 1559, 1710
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 105, 138, 336, 1237
HapII CCGG 1 cut(s) 1017
HgaI GACGC 1 cut(s) 951
HhaI GCGC 4 cut(s) 164, 676, 799, 847
Hin1I GRCGYC 1 cut(s) 162
Hin6I GCGC 4 cut(s) 162, 674, 797, 845
HinP1I GCGC 4 cut(s) 162, 674, 797, 845
HindIII AAGCTT 1 cut(s) 1652
HinfI GANTC 6 cut(s) 446, 731, 816, 907, 1764, 1803
HpaII CCGG 1 cut(s) 1017
HphI GGTGA 5 cut(s) 206, 356, 766, 1021, 1742
Hpy166II GTNNAC 6 cut(s) 115, 785, 993, 1123, 1253, 1399
Hpy188I TCNGA 6 cut(s) 321, 451, 736, 883, 982, 1670
Hpy188III TCNNGA 2 cut(s) 1059, 1761
Hpy8I GTNNAC 6 cut(s) 115, 785, 993, 1123, 1253, 1399
Hpy99I CGWCG 1 cut(s) 556
HpyAV CCTTC 4 cut(s) 290, 1384, 1555, 1675
HpyCH4III ACNGT 6 cut(s) 760, 1120, 1257, 1340, 1646, 1829
HpyCH4IV ACGT 3 cut(s) 514, 546, 1794
HpyF3I CTNAG 6 cut(s) 49, 205, 425, 606, 690, 1718
HpySE526I ACGT 3 cut(s) 514, 546, 1794
Hsp92I GRCGYC 1 cut(s) 162
HspAI GCGC 4 cut(s) 162, 674, 797, 845
KasI GGCGCC 1 cut(s) 161
Ksp22I TGATCA 3 cut(s) 346, 948, 1030
Kzo9I GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
LguI GCTCTTC 1 cut(s) 1433
LmnI GCTCC 1 cut(s) 873
Lsp1109I GCAGC 3 cut(s) 781, 1316, 1577
LweI GCATC 4 cut(s) 54, 312, 874, 1052
MaeI CTAG 5 cut(s) 26, 101, 800, 830, 1587
MaeII ACGT 3 cut(s) 514, 546, 1794
MaeIII GTNAC 5 cut(s) 287, 754, 1533, 1545, 1705
MalI GATC 7 cut(s) 198, 348, 950, 979, 1032, 1167, 1686
MbiI CCGCTC 1 cut(s) 805
MboI GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
MhlI GDGCHC 5 cut(s) 372, 787, 1255, 1445, 1827
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 2 cut(s) 423, 1436
Mph1103I ATGCAT 2 cut(s) 327, 973
MroXI GAANNNNTTC 3 cut(s) 1100, 1698, 1802
MseI TTAA 6 cut(s) 77, 213, 741, 1418, 1677, 1815
MslI CAYNNNNRTG 2 cut(s) 122, 485
MspA1I CMGCKG 2 cut(s) 209, 1568
MspI CCGG 1 cut(s) 1017
MspR9I CCNGG 2 cut(s) 1446, 1689
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 2 cut(s) 1446, 1689
MvnI CGCG 1 cut(s) 845
NarI GGCGCC 1 cut(s) 162
NdeI CATATG 2 cut(s) 123, 958
NdeII GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
NheI GCTAGC 1 cut(s) 829
NlaIV GGNNCC 3 cut(s) 163, 875, 1329
NmuCI GTSAC 2 cut(s) 754, 1705
NsbI TGCGCA 1 cut(s) 675
NsiI ATGCAT 2 cut(s) 327, 973
NspI RCATGY 5 cut(s) 484, 783, 975, 1387, 1534
PaeI GCATGC 1 cut(s) 975
PceI AGGCCT 1 cut(s) 336
PciI ACATGT 2 cut(s) 480, 1530
PciSI GCTCTTC 1 cut(s) 1433
PcsI WCGNNNNNNNCGW 3 cut(s) 560, 1183, 1791
PctI GAATGC 1 cut(s) 565
PdmI GAANNNNTTC 3 cut(s) 1100, 1698, 1802
PfeI GAWTC 5 cut(s) 731, 816, 907, 1764, 1803
PfoI TCCNGGA 1 cut(s) 1687
PkrI GCNGC 3 cut(s) 796, 1306, 1567
PleI GAGTC 1 cut(s) 454
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 454
Ppu21I YACGTR 1 cut(s) 515
PscI ACATGT 2 cut(s) 480, 1530
Psp6I CCWGG 2 cut(s) 1444, 1687
PspGI CCWGG 2 cut(s) 1444, 1687
PspN4I GGNNCC 3 cut(s) 163, 875, 1329
PspPI GGNCC 3 cut(s) 112, 127, 1235
PsrI GAACNNNNNNTAC 2 cut(s) 753, 785
PstI CTGCAG 1 cut(s) 1306
PstNI CAGNNNCTG 1 cut(s) 986
PvuII CAGCTG 2 cut(s) 209, 1568
RsaI GTAC 4 cut(s) 7, 182, 254, 1578
RsaNI GTAC 4 cut(s) 6, 181, 253, 1577
RseI CAYNNNNRTG 2 cut(s) 122, 485
SapI GCTCTTC 1 cut(s) 1433
SaqAI TTAA 6 cut(s) 77, 213, 741, 1418, 1677, 1815
SatI GCNGC 3 cut(s) 795, 1305, 1566
Sau3AI GATC 7 cut(s) 196, 346, 948, 977, 1030, 1165, 1684
Sau96I GGNCC 3 cut(s) 112, 127, 1235
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 2 cut(s) 1446, 1689
SduI GDGCHC 5 cut(s) 372, 787, 1255, 1445, 1827
SfaNI GCATC 4 cut(s) 54, 312, 874, 1052
SfcI CTRYAG 1 cut(s) 1302
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 2 cut(s) 112, 127
SmiMI CAYNNNNRTG 2 cut(s) 122, 485
SmlI CTYRAG 4 cut(s) 371, 430, 1425, 1512
SmoI CTYRAG 4 cut(s) 371, 430, 1425, 1512
SphI GCATGC 1 cut(s) 975
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 5 cut(s) 572, 805, 833, 843, 975
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 2 cut(s) 397, 640
SspMI CTAG 5 cut(s) 26, 101, 800, 830, 1587
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 1444, 1687
TaaI ACNGT 6 cut(s) 760, 1120, 1257, 1340, 1646, 1829
TaiI ACGT 3 cut(s) 517, 549, 1797
TaqI TCGA 7 cut(s) 141, 260, 392, 525, 1104, 1270, 1762
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 5 cut(s) 731, 816, 907, 1764, 1803
Tru1I TTAA 6 cut(s) 77, 213, 741, 1418, 1677, 1815
Tru9I TTAA 6 cut(s) 77, 213, 741, 1418, 1677, 1815
TscAI CASTG 7 cut(s) 899, 958, 1206, 1260, 1345, 1651, 1832
TseFI GTSAC 2 cut(s) 754, 1705
TseI GCWGC 3 cut(s) 794, 1304, 1565
Tsp45I GTSAC 2 cut(s) 754, 1705
TspRI CASTG 7 cut(s) 899, 958, 1206, 1260, 1345, 1651, 1832
VneI GTGCAC 2 cut(s) 783, 1251
VpaK11BI GGWCC 2 cut(s) 112, 127
XapI RAATTY 6 cut(s) 233, 309, 925, 1410, 1524, 1722
XceI RCATGY 5 cut(s) 484, 783, 975, 1387, 1534
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 1122
XmnI GAANNNNTTC 3 cut(s) 1100, 1698, 1802
XspI CTAG 5 cut(s) 26, 101, 800, 830, 1587
Zsp2I ATGCAT 2 cut(s) 327, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.