Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0016091

Physical Location & Seq

Genomic Coordinates Reverse (-)

17152074 .. 17154353

Gene Structure 2280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00297210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGCATGCTTATTTGATCAAAAAGGGATTTGATTTGGATGTATATTTGAATTGTGCATTGATTAATTTTTATGGGACTTGTTTGGGCATAGAAAGTTCGAACCAGTTATTTGATGAAATGCATAAGAAAGAAGATATGTTGTGGGATGAGGTTATCAAGCTCAACTCAAAGGAAGGGAGATGGGTGAAGGTTCTAGAATTGTTCAGGAATATGCAGTTGTCATTTGCTAAACCCAATAGTGCTACGATTATCAATGCGCTTCAAGCTTGTGGGAAAGTGAGAGCACTCAATGAAGGAAAGCAAATTCATGGGTATGTTTTAAGATGGGGATTAGAATCGAATTTTTCAATATGCAATTCCCTTATTAGCATGTACTCCAGAAATGGCAGACTAGAGTTAGCTAAAGCTGTTTTTGATTCAATGAATGATCATAACCTATCGTCATGGAACTCGATTATCTCTTCTTATACTGCACTTGGTTGCTTGAATGATGCTTGGATTGTTTACTATGAAATGGAGTTGTCTGGTGTCAAGCCGGACATTGTGACTTGGAATTGCCTTTTGTCCGCAGTTTCCGAATTGTGTTTGTTGAAACATGGGAAAGAAATTCATGGATTTGTAATTAGAAATGGGTTCGACTATGATGTTTATGTAGGAACTTCTTTAGTAGACATGTATGTGAAGAATAATTGTTTGTCCTTTGCTCAAAGTGTCTTTGATAACATGAAGAACAAAAACATTTTTGCTTGGAATTCCTTGATATCAGGGTATTCCTTCAAGGGTCTTTTTGAAGATGCTGAACAGCTATTAAACAGTATGAGTCAAGAAGGAATCAAACCGGACTTGGTGACATGGAATGGTTTGATTTCGGGTTGTTCACAAAATGAGAATCATGCCGAATCCCTCAAGTATTTTCTCCAAATGCAAGAAGATGGTATCAGGGCAAACTTTGCCACCATATCCAGCCTACTTAAAGCTTGTGCAGGACTATCTTTGTTGCGTAAAGGTGAAGAGATACACTGTCACAGCATACGGAAAGGTTTCGTTGAGGATGTATTTGTAGCCACAGCACTCATCGATATGTACAGCAAGGCAGGCAATTTCAGAAGTGCCTATGAGGTCTTTAGGATGATTAAAAACAAGACATTAGCTTCTTGGAATTGCATGATTATGGGATTTGCTACTTATGGCTCTGCAAAAGAGGCCATTTCTCTTTTCAATGAAATGTGCAGAGCTGGTCTGCAGCCAGATGCTATAACCTTCACAGCTTTGCTTTCTGGTTGCAAGAACTCAGGTTTAGTCGATGAAGGATGGAAGTTCTTCGATAGTATGAGCACAGATTACAACATAGCTCCAACTATTGAGCATTTCTCTTGCATGGTCGATATTCTTGCAAGAGCTGGTTATCTTGATGAAGCTTGGGATTTTATTCAAACGATGCCATTAAAGCCAGACGCCTCTATATGGGGTGCTTTTCTTGCTTCCTGCCTAACCCATAAGAACTTGGAGTTTGCAGAGACTGCAGCAAAGAATCTTTTTGAGTTGGAACCTCATAATCCAGCCAATTATGTCTTGATGATGAACTTATATTCTATATCAAACAGATGGGCGGATGTCGAACGCCTTAAAGATTTGATGAGGAAAGTAGGGGTGAAACACAGCCCTGTCTGGAGCTGGACACAAATTGATCAGAAAATCCATTTATTCTGCGCAGAAGGGAAACCTCATCCAGACGTGGGAGAAATATATTTTGAATTGTATCATTTGGTGTCCGAAATGAAGAAATTAGGCTATGAGCCTGACATTAGCTGTGTACATCAAAACATTGATGCCGTAGAGAAGAAGAAGATGCTGCTGAGTCACACAGAGAAACTGGCCATTACCTATGGACTGATGAAGATGAAAAATGGAGAACCTATCAGGGTGGTTAATAACACAAGGGTCTGTTCTGATTGTCATACTGCAGCAAAATACATGTCAGTGGTGCGAAAATGTGAGATTTTCCTCAAGGATGGTACTCGGTTTCACCACTTCAGGGAAGGGAAGTGCACCTGCAATGACTGCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

689

Amino Acids

78.25

Weight (kDa)

6.33

Isoelectric Point (pI)

36.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	145 - 190	1.4e-09	PPR repeat family
PPR_2	PF13041	245 - 293	6.5e-14	PPR repeat family
PPR	PF01535	249 - 278	8.2e-07	PPR repeat
PPR_3	PF13812	269 - 328	4.4e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	280 - 328	2.4e-08	PPR repeat family
PPR	PF01535	283 - 313	5.8e-06	PPR repeat
PPR_2	PF13041	384 - 429	5.6e-13	PPR repeat family
PPR	PF01535	385 - 414	7.3e-06	PPR repeat
E_motif	PF20431	499 - 561	7.2e-18	E motif
DYW_deaminase	PF14432	597 - 689	5.6e-35	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01030
fragaria_vesca	FvH4_7g16210
malus_domestica	MD01G1078800.v1.1
prunus_persica	Prupe.2G185700_v2.0.a1 Prupe.2G185700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0359241
rosa_laevigata	RLG00000027892
rosa_multiflora	Rmu_ssc0000135.1_g000027
rosa_roxburghii	Rroxscaffold_4G00297210
rosa_rugosa	Rorug01G0268800
rosa_samantha	Rh1AG282600 Rh1BG249000 Rh1CG266700 Rh1DG277800
rosa_wichuraiana	Rw1G025080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2060
Acc16I	TGCGCA	1 cut(s)	1711
Acc36I	ACCTGC	1 cut(s)	2060
AccI	GTMKAC	1 cut(s)	669
AciI	CCGC	2 cut(s)	567, 1610
AcoI	YGGCCR	1 cut(s)	1875
AcsI	RAATTY	4 cut(s)	303, 340, 606, 751
AcuI	CTGAAG	1 cut(s)	2017
AcyI	GRCGYC	1 cut(s)	1455
AfaI	GTAC	4 cut(s)	374, 1085, 1815, 2017
AfiI	CCNNNNNNNGG	3 cut(s)	1464, 1736, 2035
AflIII	ACRYGT	2 cut(s)	672, 1974
AjiI	CACGTC	1 cut(s)	1735
Alw21I	GWGCWC	3 cut(s)	286, 1337, 2051
Alw26I	GTCTC	1 cut(s)	1511
Alw44I	GTGCAC	1 cut(s)	2047
AlwNI	CAGNNNCTG	1 cut(s)	1520
AoxI	GGCC	2 cut(s)	1203, 1875
ApaLI	GTGCAC	1 cut(s)	2047
ApeKI	GCWGC	4 cut(s)	1243, 1523, 1852, 1964
ApoI	RAATTY	4 cut(s)	303, 340, 606, 751
ArsI	GACNNNNNNTTYG	2 cut(s)	1961, 1993
AseI	ATTAAT	1 cut(s)	63
Asp700I	GAANNNNTTC	2 cut(s)	1040, 1319
AspLEI	GCGC	2 cut(s)	259, 1712
AsuHPI	GGTGA	5 cut(s)	196, 859, 1019, 1663, 2018
AsuII	TTCGAA	1 cut(s)	98
BaeGI	GKGCMC	1 cut(s)	2051
BalI	TGGCCA	1 cut(s)	1877
Bbv12I	GWGCWC	3 cut(s)	286, 1337, 2051
BbvI	GCAGC	4 cut(s)	1255, 1535, 1839, 1976
BccI	CCATC	6 cut(s)	174, 318, 926, 1305, 1599, 2006
BceAI	ACGGC	1 cut(s)	1817
BcgI	CGANNNNNNTGC	2 cut(s)	1373, 1407
BclI	TGATCA	3 cut(s)	15, 427, 1687
BcoDI	GTCTC	1 cut(s)	1511
BfaI	CTAG	2 cut(s)	194, 392
BfmI	CTRYAG	3 cut(s)	1241, 1521, 1962
BfuAI	ACCTGC	1 cut(s)	2060
BisI	GCNGC	4 cut(s)	1244, 1524, 1853, 1965
BlsI	GCNGC	4 cut(s)	1245, 1525, 1854, 1966
BmgBI	CACGTC	1 cut(s)	1735
BmiI	GGNNCC	1 cut(s)	1548
BmsI	GCATC	6 cut(s)	481, 784, 1240, 1428, 1819, 1839
BplI	GAGNNNNNCTC	2 cut(s)	1355, 1387
BpmI	CTGGAG	2 cut(s)	361, 1690
Bpu14I	TTCGAA	1 cut(s)	98
BpuEI	CTTGAG	2 cut(s)	890, 1991
Bsa29I	ATCGAT	1 cut(s)	1077
BsaBI	GATNNNNATC	1 cut(s)	334
BsaHI	GRCGYC	1 cut(s)	1455
BsaWI	WCCGGW	1 cut(s)	838
Bsc4I	CCNNNNNNNGG	3 cut(s)	1464, 1736, 2035
Bse1I	ACTGG	2 cut(s)	103, 1878
Bse3DI	GCAATG	1 cut(s)	2062
Bse8I	GATNNNNATC	1 cut(s)	334
BseCI	ATCGAT	1 cut(s)	1077
BseGI	GGATG	8 cut(s)	43, 151, 1057, 1134, 1316, 1618, 1726, 2017
BseJI	GATNNNNATC	1 cut(s)	334
BseLI	CCNNNNNNNGG	3 cut(s)	1464, 1736, 2035
BseMI	GCAATG	1 cut(s)	2062
BseMII	CTCAG	2 cut(s)	1305, 1847
BseNI	ACTGG	2 cut(s)	103, 1878
BseSI	GKGCMC	1 cut(s)	2051
BseXI	GCAGC	4 cut(s)	1255, 1535, 1839, 1976
BsgI	GTGCAG	3 cut(s)	456, 1002, 1249
BshFI	GGCC	2 cut(s)	1205, 1877
BshVI	ATCGAT	1 cut(s)	1077
BsiHKAI	GWGCWC	3 cut(s)	286, 1337, 2051
BsiSI	CCGG	2 cut(s)	536, 839
BslFI	GGGAC	1 cut(s)	88
BslI	CCNNNNNNNGG	3 cut(s)	1464, 1736, 2035
BsmAI	GTCTC	1 cut(s)	1511
BsmFI	GGGAC	1 cut(s)	88
BsnI	GGCC	2 cut(s)	1205, 1877
Bsp119I	TTCGAA	1 cut(s)	98
Bsp1286I	GDGCHC	3 cut(s)	286, 1337, 2051
Bsp1407I	TGTACA	2 cut(s)	1083, 1813
Bsp143I	GATC	3 cut(s)	15, 427, 1687
BspACI	CCGC	2 cut(s)	567, 1610
BspANI	GGCC	2 cut(s)	1205, 1877
BspCNI	CTCAG	2 cut(s)	1304, 1848
BspDI	ATCGAT	1 cut(s)	1077
BspLI	GGNNCC	1 cut(s)	1548
BspMAI	CTGCAG	3 cut(s)	1245, 1525, 1966
BspMI	ACCTGC	1 cut(s)	2060
BspT104I	TTCGAA	1 cut(s)	98
BsrDI	GCAATG	1 cut(s)	2062
BsrGI	TGTACA	2 cut(s)	1083, 1813
BsrI	ACTGG	2 cut(s)	103, 1878
BssMI	GATC	3 cut(s)	15, 427, 1687
BssNI	GRCGYC	1 cut(s)	1455
Bst4CI	ACNGT	2 cut(s)	815, 1022
Bst6I	CTCTTC	2 cut(s)	466, 1005
BstACI	GRCGYC	1 cut(s)	1455
BstAPI	GCANNNNNTGC	3 cut(s)	950, 1520, 2061
BstAUI	TGTACA	2 cut(s)	1083, 1813
BstBI	TTCGAA	1 cut(s)	98
BstC8I	GCNNGC	2 cut(s)	6, 1096
BstDEI	CTNAG	2 cut(s)	1291, 1856
BstF5I	GGATG	8 cut(s)	43, 151, 1057, 1134, 1316, 1618, 1726, 2017
BstHHI	GCGC	2 cut(s)	259, 1712
BstKTI	GATC	3 cut(s)	18, 430, 1690
BstMAI	GTCTC	1 cut(s)	1511
BstMBI	GATC	3 cut(s)	15, 427, 1687
BstMWI	GCNNNNNNNGC	8 cut(s)	263, 950, 1095, 1202, 1447, 1478, 1520, 2061
BstNSI	RCATGY	4 cut(s)	8, 373, 676, 1978
BstSFI	CTRYAG	3 cut(s)	1241, 1521, 1962
BstSLI	GKGCMC	1 cut(s)	2051
BstV1I	GCAGC	4 cut(s)	1255, 1535, 1839, 1976
Bsu15I	ATCGAT	1 cut(s)	1077
BsuRI	GGCC	2 cut(s)	1205, 1877
BsuTUI	ATCGAT	1 cut(s)	1077
BtrI	CACGTC	1 cut(s)	1735
BtsCI	GGATG	8 cut(s)	43, 151, 1057, 1134, 1316, 1618, 1726, 2017
BtsIMutI	CAGTG	2 cut(s)	1018, 1986
BveI	ACCTGC	1 cut(s)	2060
Cac8I	GCNNGC	2 cut(s)	6, 1096
CaiI	CAGNNNCTG	1 cut(s)	1520
CfoI	GCGC	2 cut(s)	259, 1712
ClaI	ATCGAT	1 cut(s)	1077
CseI	GACGC	1 cut(s)	1463
Csp6I	GTAC	4 cut(s)	373, 1084, 1814, 2016
CviQI	GTAC	4 cut(s)	373, 1084, 1814, 2016
DdeI	CTNAG	2 cut(s)	1291, 1856
DpnI	GATC	3 cut(s)	17, 429, 1689
DpnII	GATC	3 cut(s)	15, 427, 1687
EaeI	YGGCCR	1 cut(s)	1875
Eam1104I	CTCTTC	2 cut(s)	466, 1005
EarI	CTCTTC	2 cut(s)	466, 1005
EciI	GGCGGA	1 cut(s)	1625
Eco32I	GATATC	1 cut(s)	762
Eco57I	CTGAAG	1 cut(s)	2017
EcoRI	GAATTC	1 cut(s)	751
EcoRV	GATATC	1 cut(s)	762
EcoT22I	ATGCAT	2 cut(s)	6, 123
FaqI	GGGAC	1 cut(s)	88
FbaI	TGATCA	3 cut(s)	15, 427, 1687
FblI	GTMKAC	1 cut(s)	669
Fnu4HI	GCNGC	4 cut(s)	1244, 1524, 1853, 1965
FokI	GGATG	8 cut(s)	50, 158, 1064, 1141, 1323, 1625, 1713, 2024
Fsp4HI	GCNGC	4 cut(s)	1244, 1524, 1853, 1965
FspBI	CTAG	2 cut(s)	194, 392
FspI	TGCGCA	1 cut(s)	1711
GlaI	GCGC	2 cut(s)	258, 1711
GluI	GCNGC	4 cut(s)	1244, 1524, 1853, 1965
GsuI	CTGGAG	2 cut(s)	361, 1690
HaeIII	GGCC	2 cut(s)	1205, 1877
HapII	CCGG	2 cut(s)	536, 839
HgaI	GACGC	1 cut(s)	1463
HhaI	GCGC	2 cut(s)	259, 1712
Hin1I	GRCGYC	1 cut(s)	1455
Hin6I	GCGC	2 cut(s)	257, 1710
HinP1I	GCGC	2 cut(s)	257, 1710
HindIII	AAGCTT	3 cut(s)	264, 975, 1416
HinfI	GANTC	8 cut(s)	335, 416, 820, 831, 889, 899, 1531, 1858
HpaII	CCGG	2 cut(s)	536, 839
HphI	GGTGA	5 cut(s)	196, 859, 1019, 1663, 2018
Hpy166II	GTNNAC	5 cut(s)	505, 670, 878, 1814, 2049
Hpy188I	TCNGA	5 cut(s)	577, 1106, 1692, 1774, 1951
Hpy188III	TCNNGA	8 cut(s)	194, 205, 378, 824, 1409, 1573, 1669, 1730
Hpy8I	GTNNAC	5 cut(s)	505, 670, 878, 1814, 2049
HpyAV	CCTTC	9 cut(s)	167, 181, 287, 784, 821, 1270, 1301, 1709, 2033
HpyCH4III	ACNGT	2 cut(s)	815, 1022
HpyCH4IV	ACGT	1 cut(s)	1734
HpyF10VI	GCNNNNNNNGC	8 cut(s)	263, 950, 1095, 1202, 1447, 1478, 1520, 2061
HpyF3I	CTNAG	2 cut(s)	1291, 1856
HpySE526I	ACGT	1 cut(s)	1734
Hsp92I	GRCGYC	1 cut(s)	1455
HspAI	GCGC	2 cut(s)	257, 1710
Ksp22I	TGATCA	3 cut(s)	15, 427, 1687
Kzo9I	GATC	3 cut(s)	15, 427, 1687
LmnI	GCTCC	2 cut(s)	1357, 1671
Lsp1109I	GCAGC	4 cut(s)	1255, 1535, 1839, 1976
LweI	GCATC	6 cut(s)	481, 784, 1240, 1428, 1819, 1839
MaeI	CTAG	2 cut(s)	194, 392
MaeII	ACGT	1 cut(s)	1734
MaeIII	GTNAC	4 cut(s)	544, 847, 1022, 1859
MalI	GATC	3 cut(s)	17, 429, 1689
MboI	GATC	3 cut(s)	15, 427, 1687
MhlI	GDGCHC	3 cut(s)	286, 1337, 2051
MlsI	TGGCCA	1 cut(s)	1877
MluNI	TGGCCA	1 cut(s)	1877
MlyI	GAGTC	2 cut(s)	829, 1867
MmeI	TCCRAC	2 cut(s)	1379, 1524
Mox20I	TGGCCA	1 cut(s)	1877
Mph1103I	ATGCAT	2 cut(s)	6, 123
MroXI	GAANNNNTTC	2 cut(s)	1040, 1319
MscI	TGGCCA	1 cut(s)	1877
MseI	TTAA	8 cut(s)	63, 320, 809, 972, 1134, 1445, 1626, 1929
MslI	CAYNNNNRTG	5 cut(s)	312, 677, 1079, 1169, 1979
Msp20I	TGGCCA	1 cut(s)	1877
MspI	CCGG	2 cut(s)	536, 839
MwoI	GCNNNNNNNGC	8 cut(s)	263, 950, 1095, 1202, 1447, 1478, 1520, 2061
NdeII	GATC	3 cut(s)	15, 427, 1687
NlaIV	GGNNCC	1 cut(s)	1548
NmuCI	GTSAC	4 cut(s)	544, 847, 1022, 1859
NsbI	TGCGCA	1 cut(s)	1711
NsiI	ATGCAT	2 cut(s)	6, 123
NspI	RCATGY	4 cut(s)	8, 373, 676, 1978
NspV	TTCGAA	1 cut(s)	98
PaeI	GCATGC	1 cut(s)	8
PaqCI	CACCTGC	1 cut(s)	2060
PciI	ACATGT	2 cut(s)	672, 1974
PdmI	GAANNNNTTC	2 cut(s)	1040, 1319
PfeI	GAWTC	6 cut(s)	335, 416, 831, 889, 899, 1531
PkrI	GCNGC	4 cut(s)	1245, 1525, 1854, 1966
PleI	GAGTC	2 cut(s)	828, 1866
PpsI	GAGTC	2 cut(s)	828, 1866
PscI	ACATGT	2 cut(s)	672, 1974
PshBI	ATTAAT	1 cut(s)	63
PspN4I	GGNNCC	1 cut(s)	1548
PstI	CTGCAG	3 cut(s)	1245, 1525, 1966
PstNI	CAGNNNCTG	1 cut(s)	1520
RsaI	GTAC	4 cut(s)	374, 1085, 1815, 2017
RsaNI	GTAC	4 cut(s)	373, 1084, 1814, 2016
RseI	CAYNNNNRTG	5 cut(s)	312, 677, 1079, 1169, 1979
SaqAI	TTAA	8 cut(s)	63, 320, 809, 972, 1134, 1445, 1626, 1929
SatI	GCNGC	4 cut(s)	1244, 1524, 1853, 1965
Sau3AI	GATC	3 cut(s)	15, 427, 1687
SchI	GAGTC	2 cut(s)	829, 1867
SduI	GDGCHC	3 cut(s)	286, 1337, 2051
SfaNI	GCATC	6 cut(s)	481, 784, 1240, 1428, 1819, 1839
SfcI	CTRYAG	3 cut(s)	1241, 1521, 1962
SfuI	TTCGAA	1 cut(s)	98
SmiMI	CAYNNNNRTG	5 cut(s)	312, 677, 1079, 1169, 1979
SmlI	CTYRAG	2 cut(s)	905, 2006
SmoI	CTYRAG	2 cut(s)	905, 2006
SphI	GCATGC	1 cut(s)	8
SsiI	CCGC	2 cut(s)	567, 1610
SspMI	CTAG	2 cut(s)	194, 392
TaaI	ACNGT	2 cut(s)	815, 1022
TaiI	ACGT	1 cut(s)	1737
TaqI	TCGA	9 cut(s)	98, 338, 452, 636, 1077, 1302, 1323, 1383, 1617
TatI	WGTACW	3 cut(s)	372, 1083, 1813
TfiI	GAWTC	6 cut(s)	335, 416, 831, 889, 899, 1531
Tru1I	TTAA	8 cut(s)	63, 320, 809, 972, 1134, 1445, 1626, 1929
Tru9I	TTAA	8 cut(s)	63, 320, 809, 972, 1134, 1445, 1626, 1929
TscAI	CASTG	2 cut(s)	1025, 1986
TseFI	GTSAC	4 cut(s)	544, 847, 1022, 1859
TseI	GCWGC	4 cut(s)	1243, 1523, 1852, 1964
Tsp45I	GTSAC	4 cut(s)	544, 847, 1022, 1859
TspGWI	ACGGA	1 cut(s)	1048
TspRI	CASTG	2 cut(s)	1025, 1986
VneI	GTGCAC	1 cut(s)	2047
VspI	ATTAAT	1 cut(s)	63
XapI	RAATTY	4 cut(s)	303, 340, 606, 751
XbaI	TCTAGA	1 cut(s)	193
XceI	RCATGY	4 cut(s)	8, 373, 676, 1978
XmiI	GTMKAC	1 cut(s)	669
XmnI	GAANNNNTTC	2 cut(s)	1040, 1319
XspI	CTAG	2 cut(s)	194, 392
Zsp2I	ATGCAT	2 cut(s)	6, 123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.