Rh1AG282600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016091
Physical Location & Seq
Forward (+)
51178268 .. 51181194
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG282600.1

Sequence Viewer

Length: 2328 bp
ATGGCCAAACTAGCTCCATCTCACCTCCTTCAAACCCCATTAAGCTCCAAGCCAACATCTTTGCCTAGACTGTCTTCAACCCTTACCTTGTTGGAGGACTTGGGTCAGTTGGGTGAGCTCAAGATTTTGAGTTCAGTGAAAGCAGTCCATGCCCAGATGATAAAAATATCCAACAACAACAAAATGGATATAAAGGCAAAATCTTTGGCCACTTGTTACTTGGAATTTGGTGATTCTAGGTCAGCTGCAATGGCTTTCTTTGTGGGTTCTGCACATTATCTTCCATGGAGTTCTTTTCTAGAAGAGTTTAGAAGATATGGGAATGACCCAGAAGATGTTCTCAAGTTCTTTCTTGAGTTTCATAATAGGGGAGTAGTGTTTGATAGCAGAGTTCTTGGTTTTGTTTTGAAACTTTGTGGGAATTTGAAGGAGTTGTGGCTTGGGTTGGAAATGCATGCTTATTTGATCAAAAAGGGATTTGATTTGGATGTATATTTGAATTGTGCATTGATTAATTTTTATGGGACTTGTTTGGGCATAGAAAGTTCGAACCAGTTATTTGATGAAATGCATAAGAAAGAAGATATGTTGTGGGATGAGGTTATTAAGCTCAACTCAAAGAAAGGGAGATGGGTGAAGGTTCTAGAATTGTTCAGGAATATGCAGCTGTCATTTGCTAAACCCAATAGTGCTACGATTATCAATGCGCTTCAAGCTTGTGGGAAAGTGAGAGCACTCAATGAAGGAAAGCAAATTCATGGGTATGTTTTAAGATGGGGATTAGAATCGAATTTGTCAATATGCAATTCCCTTATTAGCATGTACTCCAGAAATGGCAGACTAGAATTAGCTAAAGCTGTTTTTGATTCAATGAATGATCATAACTTATCGTCATGGAACTCGATTATCTCTTCTTATACTGCACTTGGTTGCTTGAATGATGCTTGGATTGTTTACTATGAAATGGAGTTGTCTGATGTCAAGCCGGACATTGTGACTTGGAATTGCCTTTTGTCCGGTCATTCTCTTCACGGCTCGTATCAAGAAGTACTTGCCATCTTGAAGAGAATGCAAGATGCCGGGTTCAAGCCAAATTCAAGTTCCATTACTAGTGTTCTTCAAGCAGTTTCTGAATTGTGTTTGTTGAAACATGGGAAAGAAATTCATGCATTTGTAATTAGAAATGGGCTCGACTATGATGTTTATGTAGGAACTTCTTTAGTAGACATGTATGTGAAGAATAATTGTTTGTCCTTTGCTCAAAGTGTCTTTGGTAACATGAAGAACAAAAACATTTTTGCTTGGAATTCCTTGATATCAGGGTATTCCTTCAAGGGTCTTTTTGAAGATGCTGAACAGCTATTAAACAGTATGAGTCAAGAAGGAATCAAACCGGACTTAGTGACATGGAATGGTTTGATTTCGGGGTATGCAATGAAGGGTCGCCATAAGGAAGCTATAGCGGCAATTCATCGGATGAAAAGTTCAGGGTTAACCCCTAATGTGGTTTCATGGACTGCTCTTATATCAGGTTGTTCACAAAATGAGAATCATGCCGAATCCCTCAAGTATTTTCTCCAAATGCAAGAAGATGGTATCAGGGCAAACTTTGCCACCATATCCAGCCTACTTAAAGCTTGTGCAGGACTATCTTTGTTGCGTAAGGGTGAAGAGATACACTGTCACAGCATACGGAAAGGTTTCATTGAGGATGTATTTGTAGCCACAGCACTCGTCGATATGTACAGCAAGGCAGGCAATTTCAGAAGTGCCTATGAGGTCTTTAGGATGATTAAAAACAGGACATTAGCTTCTTGGAATTGCATGACTATGGGATTTGCTACTTATGGCTCTGCAAAAGAGGCCATTTCTCTTTTCAATGAAATGCGCGGAGCTGGTCTGCAGCCAGATGCTATAACCTTCACAGCTTTGCTTTCTGGTTGCAAGAACTCAGGTTTAGTCAATGAAGGATGGAAGTTCTTCGATAGTATGAGCACAGATTACAACATAGCTCCAACTATTGAGCATTTCTCTTGCATGGTAGATATTCTTGCAAGAGCTGGTTATCTTGATGAAGCTTGGGATTTTATTCAAACGATGCCATTAAAGCCGGACTCCTCTATATGGGGTGCTTTTCTTGCTTCCTGCCTAACCCATAAGAACTTGGAGTTTGCAGAGACTGCAGCAAAGAATCTTTTTGAATTGGAACCTCATAATCCAGCCAATTATGTCTTGATGATGAACTTATATTCTATATCAAACAGATGGGCGGATGTCGAACGCCTTAAAGATTTGATGAGGAAAGTAGGGGTGAAACACAGCCCTTGA

Protein Analysis

775

Amino Acids

86.89

Weight (kDa)

7.54

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 320 - 368 3.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 329 - 375 1.8e-08 PPR repeat family
PPR_2 PF13041 430 - 478 2.1e-13 PPR repeat family
PPR PF01535 434 - 463 9.5e-07 PPR repeat
PPR_3 PF13812 454 - 513 1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 462 - 494 2.8e-06 PPR repeat
PPR_2 PF13041 465 - 512 1.9e-12 PPR repeat family
PPR PF01535 468 - 498 1.7e-06 PPR repeat
PPR_3 PF13812 491 - 548 3.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 500 - 548 7.5e-09 PPR repeat family
PPR PF01535 503 - 533 7e-06 PPR repeat
PPR_2 PF13041 604 - 649 5.7e-10 PPR repeat family
E_motif PF20431 719 - 775 2.2e-16 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01030
fragaria_vesca FvH4_7g16210
malus_domestica MD01G1078800.v1.1
prunus_persica Prupe.2G185700_v2.0.a1 Prupe.2G185700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359241
rosa_laevigata RLG00000027892
rosa_multiflora Rmu_ssc0000135.1_g000027
rosa_roxburghii Rroxscaffold_4G00297210
rosa_rugosa Rorug01G0268800
rosa_samantha Rh1AG282600 Rh1BG249000 Rh1CG266700 Rh1DG277800
rosa_wichuraiana Rw1G025080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1224
AccII CGCG 1 cut(s) 1890
AciI CCGC 3 cut(s) 1463, 1890, 2270
AcoI YGGCCR 2 cut(s) 3, 207
AcsI RAATTY 7 cut(s) 224, 421, 753, 790, 1093, 1161, 1306
AfaI GTAC 3 cut(s) 824, 1050, 1745
AfiI CCNNNNNNNGG 2 cut(s) 1504, 2124
AflIII ACRYGT 1 cut(s) 1227
AhlI ACTAGT 1 cut(s) 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 1084, 1116
AloI GAACNNNNNNTCC 2 cut(s) 1468, 1500
Alw21I GWGCWC 3 cut(s) 120, 736, 1997
Alw26I GTCTC 1 cut(s) 2171
AlwNI CAGNNNCTG 2 cut(s) 1130, 2180
AoxI GGCC 3 cut(s) 3, 207, 1863
ApeKI GCWGC 4 cut(s) 245, 664, 1903, 2183
ApoI RAATTY 7 cut(s) 224, 421, 753, 790, 1093, 1161, 1306
AseI ATTAAT 1 cut(s) 513
Asp700I GAANNNNTTC 3 cut(s) 336, 1700, 1979
AspLEI GCGC 2 cut(s) 709, 1890
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 6 cut(s) 14, 125, 242, 646, 1679, 2323
AsuII TTCGAA 1 cut(s) 548
BalI TGGCCA 2 cut(s) 5, 209
BanII GRGCYC 2 cut(s) 120, 1191
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 3 cut(s) 120, 736, 1997
BbvI GCAGC 4 cut(s) 232, 676, 1915, 2195
BccI CCATC 7 cut(s) 25, 624, 768, 1064, 1586, 1965, 2259
BceAI ACGGC 1 cut(s) 1048
BclI TGATCA 2 cut(s) 465, 877
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 1 cut(s) 2171
BcuI ACTAGT 1 cut(s) 1109
BfaI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
BfmI CTRYAG 3 cut(s) 1458, 1901, 2181
BisI GCNGC 5 cut(s) 246, 665, 1464, 1904, 2184
BlsI GCNGC 5 cut(s) 247, 666, 1465, 1905, 2185
BmcAI AGTACT 1 cut(s) 1050
Bme1390I CCNGG 1 cut(s) 1081
BmiI GGNNCC 1 cut(s) 2208
BmrFI CCNGG 1 cut(s) 1081
BmsI GCATC 5 cut(s) 931, 1066, 1339, 1900, 2088
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 2015, 2047
BpmI CTGGAG 1 cut(s) 811
Bpu14I TTCGAA 1 cut(s) 548
BpuEI CTTGAG 4 cut(s) 104, 326, 374, 1550
BpuMI CCSGG 1 cut(s) 1081
BsaBI GATNNNNATC 1 cut(s) 784
BsaJI CCNNGG 1 cut(s) 284
BsaWI WCCGGW 2 cut(s) 1016, 1393
Bsc4I CCNNNNNNNGG 2 cut(s) 1504, 2124
Bse1I ACTGG 1 cut(s) 553
Bse3DI GCAATG 2 cut(s) 255, 1440
Bse8I GATNNNNATC 1 cut(s) 784
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 7 cut(s) 493, 601, 1482, 1717, 1794, 1976, 2278
BseJI GATNNNNATC 1 cut(s) 784
BseLI CCNNNNNNNGG 2 cut(s) 1504, 2124
BseMI GCAATG 2 cut(s) 255, 1440
BseMII CTCAG 1 cut(s) 1965
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 1 cut(s) 2107
BseXI GCAGC 4 cut(s) 232, 676, 1915, 2195
BsgI GTGCAG 3 cut(s) 255, 906, 1662
Bsh1236I CGCG 1 cut(s) 1890
BshFI GGCC 3 cut(s) 5, 209, 1865
BsiHKAI GWGCWC 3 cut(s) 120, 736, 1997
BsiSI CCGG 5 cut(s) 986, 1017, 1080, 1394, 2111
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 2 cut(s) 1504, 2124
BsmAI GTCTC 1 cut(s) 2171
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 1 cut(s) 1074
BsnI GGCC 3 cut(s) 5, 209, 1865
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 4 cut(s) 120, 736, 1191, 1997
Bsp1407I TGTACA 1 cut(s) 1743
Bsp143I GATC 2 cut(s) 465, 877
Bsp19I CCATGG 1 cut(s) 284
BspACI CCGC 3 cut(s) 1463, 1890, 2270
BspANI GGCC 3 cut(s) 5, 209, 1865
BspCNI CTCAG 1 cut(s) 1964
BspFNI CGCG 1 cut(s) 1890
BspLI GGNNCC 1 cut(s) 2208
BspMAI CTGCAG 2 cut(s) 1905, 2185
BspT104I TTCGAA 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 255, 1440
BsrGI TGTACA 1 cut(s) 1743
BsrI ACTGG 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 2 cut(s) 465, 877
BssT1I CCWWGG 1 cut(s) 284
Bst4CI ACNGT 3 cut(s) 72, 1370, 1682
Bst6I CTCTTC 5 cut(s) 297, 916, 1032, 1058, 1665
BstAPI GCANNNNNTGC 3 cut(s) 149, 1610, 2180
BstAUI TGTACA 1 cut(s) 1743
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 456, 1756
BstDEI CTNAG 2 cut(s) 1399, 1951
BstDSI CCRYGG 1 cut(s) 284
BstF5I GGATG 7 cut(s) 493, 601, 1482, 1717, 1794, 1976, 2278
BstFNI CGCG 1 cut(s) 1890
BstHHI GCGC 2 cut(s) 709, 1890
BstKTI GATC 2 cut(s) 468, 880
BstMAI GTCTC 1 cut(s) 2171
BstMBI GATC 2 cut(s) 465, 877
BstNSI RCATGY 3 cut(s) 458, 823, 1231
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 1079
BstSFI CTRYAG 3 cut(s) 1458, 1901, 2181
BstUI CGCG 1 cut(s) 1890
BstV1I GCAGC 4 cut(s) 232, 676, 1915, 2195
BstV2I GAAGAC 1 cut(s) 66
BsuRI GGCC 3 cut(s) 5, 209, 1865
BtgI CCRYGG 1 cut(s) 284
BtsCI GGATG 7 cut(s) 493, 601, 1482, 1717, 1794, 1976, 2278
BtsIMutI CAGTG 2 cut(s) 141, 1678
Cac8I GCNNGC 2 cut(s) 456, 1756
CaiI CAGNNNCTG 2 cut(s) 1130, 2180
CfoI GCGC 2 cut(s) 709, 1890
Csp6I GTAC 3 cut(s) 823, 1049, 1744
CviQI GTAC 3 cut(s) 823, 1049, 1744
DdeI CTNAG 2 cut(s) 1399, 1951
DpnI GATC 2 cut(s) 467, 879
DpnII GATC 2 cut(s) 465, 877
EaeI YGGCCR 2 cut(s) 3, 207
Eam1104I CTCTTC 5 cut(s) 297, 916, 1032, 1058, 1665
EarI CTCTTC 5 cut(s) 297, 916, 1032, 1058, 1665
EciI GGCGGA 1 cut(s) 2285
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 284
Eco24I GRGCYC 2 cut(s) 120, 1191
Eco32I GATATC 1 cut(s) 1317
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 1306
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 1 cut(s) 284
EcoT22I ATGCAT 3 cut(s) 456, 573, 1171
EcoT38I GRGCYC 2 cut(s) 120, 1191
ErhI CCWWGG 1 cut(s) 284
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 1 cut(s) 538
FbaI TGATCA 2 cut(s) 465, 877
FblI GTMKAC 1 cut(s) 1224
Fnu4HI GCNGC 5 cut(s) 246, 665, 1464, 1904, 2184
FokI GGATG 7 cut(s) 500, 608, 1489, 1724, 1801, 1983, 2285
FriOI GRGCYC 2 cut(s) 120, 1191
Fsp4HI GCNGC 5 cut(s) 246, 665, 1464, 1904, 2184
FspBI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
GlaI GCGC 2 cut(s) 708, 1889
GluI GCNGC 5 cut(s) 246, 665, 1464, 1904, 2184
GsuI CTGGAG 1 cut(s) 811
HaeIII GGCC 3 cut(s) 5, 209, 1865
HapII CCGG 5 cut(s) 986, 1017, 1080, 1394, 2111
HhaI GCGC 2 cut(s) 709, 1890
Hin6I GCGC 2 cut(s) 707, 1888
HinP1I GCGC 2 cut(s) 707, 1888
HincII GTYRAC 1 cut(s) 1494
HindII GTYRAC 1 cut(s) 1494
HindIII AAGCTT 3 cut(s) 714, 1635, 2076
HinfI GANTC 9 cut(s) 233, 785, 866, 1375, 1386, 1549, 1559, 2114, 2191
HpaI GTTAAC 1 cut(s) 1494
HpaII CCGG 5 cut(s) 986, 1017, 1080, 1394, 2111
HphI GGTGA 6 cut(s) 14, 125, 242, 646, 1679, 2323
Hpy166II GTNNAC 4 cut(s) 955, 1225, 1494, 1538
Hpy188I TCNGA 4 cut(s) 976, 1132, 1476, 1766
Hpy8I GTNNAC 4 cut(s) 955, 1225, 1494, 1538
Hpy99I CGWCG 1 cut(s) 1739
HpyAV CCTTC 9 cut(s) 38, 421, 631, 737, 1339, 1376, 1432, 1930, 1961
HpyCH4III ACNGT 3 cut(s) 72, 1370, 1682
HpyF3I CTNAG 2 cut(s) 1399, 1951
HspAI GCGC 2 cut(s) 707, 1888
Ksp22I TGATCA 2 cut(s) 465, 877
KspAI GTTAAC 1 cut(s) 1494
Kzo9I GATC 2 cut(s) 465, 877
LmnI GCTCC 4 cut(s) 19, 50, 1892, 2017
Lsp1109I GCAGC 4 cut(s) 232, 676, 1915, 2195
LweI GCATC 5 cut(s) 931, 1066, 1339, 1900, 2088
MaeI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
MaeIII GTNAC 5 cut(s) 215, 994, 1274, 1402, 1682
MalI GATC 2 cut(s) 467, 879
MboI GATC 2 cut(s) 465, 877
MhlI GDGCHC 4 cut(s) 120, 736, 1191, 1997
MlsI TGGCCA 2 cut(s) 5, 209
MluNI TGGCCA 2 cut(s) 5, 209
MlyI GAGTC 2 cut(s) 1384, 2108
MmeI TCCRAC 4 cut(s) 72, 195, 426, 2039
Mox20I TGGCCA 2 cut(s) 5, 209
Mph1103I ATGCAT 3 cut(s) 456, 573, 1171
MroXI GAANNNNTTC 3 cut(s) 336, 1700, 1979
MscI TGGCCA 2 cut(s) 5, 209
MslI CAYNNNNRTG 3 cut(s) 762, 1232, 1829
Msp20I TGGCCA 2 cut(s) 5, 209
MspA1I CMGCKG 2 cut(s) 245, 667
MspI CCGG 5 cut(s) 986, 1017, 1080, 1394, 2111
MspR9I CCNGG 1 cut(s) 1081
Mva1269I GAATGC 1 cut(s) 1074
MvnI CGCG 1 cut(s) 1890
NciI CCSGG 1 cut(s) 1081
NcoI CCATGG 1 cut(s) 284
NdeII GATC 2 cut(s) 465, 877
NlaIV GGNNCC 1 cut(s) 2208
NmuCI GTSAC 3 cut(s) 994, 1402, 1682
NsiI ATGCAT 3 cut(s) 456, 573, 1171
NspI RCATGY 3 cut(s) 458, 823, 1231
NspV TTCGAA 1 cut(s) 548
PaeI GCATGC 1 cut(s) 458
PciI ACATGT 1 cut(s) 1227
PctI GAATGC 1 cut(s) 1074
PdmI GAANNNNTTC 3 cut(s) 336, 1700, 1979
PfeI GAWTC 7 cut(s) 233, 785, 866, 1386, 1549, 1559, 2191
PkrI GCNGC 5 cut(s) 247, 666, 1465, 1905, 2185
PleI GAGTC 2 cut(s) 1383, 2108
PpsI GAGTC 2 cut(s) 1383, 2108
PscI ACATGT 1 cut(s) 1227
PshAI GACNNNNGTC 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 2208
PstI CTGCAG 2 cut(s) 1905, 2185
PstNI CAGNNNCTG 2 cut(s) 1130, 2180
PvuII CAGCTG 2 cut(s) 245, 667
RsaI GTAC 3 cut(s) 824, 1050, 1745
RsaNI GTAC 3 cut(s) 823, 1049, 1744
RseI CAYNNNNRTG 3 cut(s) 762, 1232, 1829
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 5 cut(s) 246, 665, 1464, 1904, 2184
Sau3AI GATC 2 cut(s) 465, 877
ScaI AGTACT 1 cut(s) 1050
SchI GAGTC 2 cut(s) 1384, 2108
ScrFI CCNGG 1 cut(s) 1081
SduI GDGCHC 4 cut(s) 120, 736, 1191, 1997
SfaNI GCATC 5 cut(s) 931, 1066, 1339, 1900, 2088
SfcI CTRYAG 3 cut(s) 1458, 1901, 2181
SfuI TTCGAA 1 cut(s) 548
SmiMI CAYNNNNRTG 3 cut(s) 762, 1232, 1829
SmlI CTYRAG 4 cut(s) 119, 341, 353, 1565
SmoI CTYRAG 4 cut(s) 119, 341, 353, 1565
SpeI ACTAGT 1 cut(s) 1109
SphI GCATGC 1 cut(s) 458
SsiI CCGC 3 cut(s) 1463, 1890, 2270
SspMI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 1079
StyI CCWWGG 1 cut(s) 284
TaaI ACNGT 3 cut(s) 72, 1370, 1682
TaqI TCGA 7 cut(s) 548, 788, 902, 1191, 1737, 1983, 2277
TatI WGTACW 3 cut(s) 822, 1048, 1743
TauI GCSGC 1 cut(s) 1466
TfiI GAWTC 7 cut(s) 233, 785, 866, 1386, 1549, 1559, 2191
TscAI CASTG 2 cut(s) 141, 1685
TseFI GTSAC 3 cut(s) 994, 1402, 1682
TseI GCWGC 4 cut(s) 245, 664, 1903, 2183
Tsp45I GTSAC 3 cut(s) 994, 1402, 1682
TspGWI ACGGA 1 cut(s) 1708
TspRI CASTG 2 cut(s) 141, 1685
VspI ATTAAT 1 cut(s) 513
XapI RAATTY 7 cut(s) 224, 421, 753, 790, 1093, 1161, 1306
XbaI TCTAGA 2 cut(s) 298, 643
XceI RCATGY 3 cut(s) 458, 823, 1231
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 1224
XmnI GAANNNNTTC 3 cut(s) 336, 1700, 1979
XspI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
ZrmI AGTACT 1 cut(s) 1050
Zsp2I ATGCAT 3 cut(s) 456, 573, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.