Rw1G025080

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016091
Physical Location & Seq
Forward (+)
51712960 .. 51715961
3002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025080.1

Sequence Viewer

Length: 2730 bp
ATGGCCAAACTAGCTCCATCTTACCTCCTTCAAAGCTCATTAAGCTCCAAGCCAACATCTTTGCCTAGACTGTCTTCAACCCTTACCTTGTTGGATGACTTGGGTCAGTTGGGTGAGCTCAAGACTTTGAGTTCAGTGAAAGCAGTCCATGCCCAGATGATAAAAATGTCCAACAACAACAAAATGGATATAAAGGCAAAATCTTTGGCCACTTGTTACCTGGAATTTGGTGATTCTAGGTCAGCTGCAATGGCTTTCTTTGTGGGTTCTGCACATTATCTTCCATGGAGTTCTTTTCTAGAAGAGTTTAGAAGATATGGGAATGACCCAGAAGATGTTCTCAAGTTCTTTCTTGAGTTTCATAATAGGGGAGTAGTGTTTGATAGCAGAGTTCTTGGTTTTGTTTTGAAACTTTGTGGGAATTTGAAGGAGTTGTGGCTTGGGTTGGAAATGCATGCTTATTTGATCAAAAAGGGATTTGATTTGGATGTATATTTGAATTGTGCATTGATTAATTTTTATGGGACTTGTTTGGGCATAGAAAGTTCGAACCAGTTATTTGATGAAATGCATAAGAAAGAAGATATGTTGTGGGATGAGGTTATTAAGCTCAACTCAAAGAAAGGGAGATGGGTGAAGGTTCTAGAATTGTTCAGGAATATGCAGCTGTCATTTGCTAAACCCAATAGTGCTACGATTATCAATGCGCTTCAAGCTTGTGGGAAAGTGAGAGCACTCAATGAAGGAAAGCAAATTCATGGGTATGTTTTAAGATGGGGATTAGAATCGAATTTGTCAATATGCAATTCCCTTATTAGCATGTACTCCAGAAATGGCAGACTAGAATTAGCTAAAGCTGTCTTTGATTCAATGAATGATCATAACTTATCGTCATGGAACTCGATTATCTCTTCTTATAATGCACTTGGTTGCTTGAATGATGCTTGGATTGTTTACTATGAAATGGAGTTGTCTGATGTCAAGCCGGACATTGTGACTTGGAATTGCCTTTTGTCAGGTCATTCTCTTCACGGCTCGTATCAAGAAGTACTTGCCATCTTGAAGAGAATACAAGATGCCGGGTTCAAGCCAAATTCAAGTTCCATTACTAGTGTTCTTCAAGCAGTTTCTGAATTGTGTTTGTTGAAACATGGGAAAGAAATTCATGCATTTGTAATTAGAAATGGGCTCGACTATGATGTTTATGTAGGAACTTCTTTAGTAGATATGTATGTGAAGAATAATTGTTTGTCCTTTGCTCAAAGTGTCTTTGATAACATGAAGAACAAAAACATTTTTGCTTGGAATTCCTTGATATCAGGGTATTCCTTCAAGGGTCTTTTTGAAGATGCTGAACAGCTATTAAACAGTATGAGTCAAGAAGGAATCAAACCGGACTTAGTGACATGGAATGGTTTGATTTCAGGGTATGCAATGAAGGGTCGCCATAAGGAAGCTATAGCGGCAATTCATCGGATGAAAAGTTCAGGGTTAACCCCTAACGTGGTTTCATGGACTGCTCTTATATCAGGTTGTTCACAAAATGAGAATCATGCTGAATCCCTCAAGTATTTTCTCCAAATGCAAGAAGATGGTATCAGGGCAAACTTTGCCACCATATCCAGCCTACTTAAAGCTTGTGCAGGACTATCTTTGTTGCGTAAGGGTGAAGAGATACACTGTCACAGCATACGGAAAGGTTTCATTGAGGATGTATTTGTAGCCACAGCACTTGTCGATATGTACAGCAAGGCAGGCAATTTCAGAAGCGCCTATGAGGTCTTTAGGATGATTAAAAACAGGACATTAGCTTCTTGGAATTGCATGATTATGGGATTTGCTATTTATGGCTCTGCAAAAGAGGCCATTTCTCTTTTCAATGAAATGCACGGAGCTGGTCTGCAGCCAGATGCTATAACCTTCACAGCTTTGCTTTCTGGTTGCAAGAACTCAGGTTTAGTCGATGAAGGATGGAAGTTCTTCGATAGTATGAGCACAGATTACAACATAGCTCCAACTATTGAGCATTTCTCTTGCATGGTAGATATTCTTGCAAGAGCTGGTTATCTTGATGAAGCTTGGGATTTTATTCAAATGATGCCATTAAAGCCGGACGCCTCTATATGGGGTGCTTTTCTTGCTTCCTGCCTAACCCATAAGAACTTGGAGTTTGCAGAGACTGCAGCAAAGAATCTTTTTGAGTTGGAACCTCATAATCCAGCCAATTATGTCTTGATGATGAACTTATATTCTATATCAAACAGATGGGCGGATGTCGAACGCCTTAAAGATTTGATGAGGAAAGTAGGGGTGAAACACAGCCCTGTATGGAGCTGGACACAAATTGATCAGAAAATCCATATGTTCTGTGCAGAAGGGAAACCTCATCCAGACTTGGGAGAAATATATTTTGAATTGTATCATTTGGTTTCCGAAATGAAGAAATTAGGCTATGAGCCTGACATTAGCTGTGTACATCAAAACATTGATGCCGTAGAGAAGAAGAAGATGCTGCTGAGTCACACAGAGAAACTGGCCATTACCTATGGACTGATGAAGATGAAAAATGGAGAACCTATCAGGGTGGTTAATAACACAAGGGTCTGTTCTGATTGTCATACTGCAGCAAAATACATGTCAGTGGTGCGAAAATGCGAGATTTTCCTCAAGGATGGTACTCGTTTTCACCACTTCAGGGAAGGGAAGTGCACCTGCAATGACTGCTGGTAA

Protein Analysis

909

Amino Acids

102.6

Weight (kDa)

7.31

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 295 - 341 4.1e-08 PPR repeat family
PPR_2 PF13041 329 - 375 1.2e-06 PPR repeat family
PPR_2 PF13041 430 - 471 1.5e-10 PPR repeat family
PPR PF01535 434 - 463 1.1e-06 PPR repeat
PPR_3 PF13812 454 - 513 1.2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 462 - 494 3.4e-06 PPR repeat
PPR_2 PF13041 465 - 512 4.1e-12 PPR repeat family
PPR PF01535 468 - 498 2.1e-06 PPR repeat
PPR_3 PF13812 491 - 548 4.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 500 - 548 9.1e-09 PPR repeat family
PPR PF01535 503 - 533 8.5e-06 PPR repeat
PPR_2 PF13041 604 - 649 3e-11 PPR repeat family
E_motif PF20431 719 - 781 1e-17 E motif
DYW_deaminase PF14432 817 - 909 8.3e-35 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01030
fragaria_vesca FvH4_7g16210
malus_domestica MD01G1078800.v1.1
prunus_persica Prupe.2G185700_v2.0.a1 Prupe.2G185700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359241
rosa_laevigata RLG00000027892
rosa_multiflora Rmu_ssc0000135.1_g000027
rosa_roxburghii Rroxscaffold_4G00297210
rosa_rugosa Rorug01G0268800
rosa_samantha Rh1AG282600 Rh1BG249000 Rh1CG266700 Rh1DG277800
rosa_wichuraiana Rw1G025080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 918
AarI CACCTGC 1 cut(s) 2720
Acc36I ACCTGC 1 cut(s) 2720
AciI CCGC 2 cut(s) 1463, 2270
AcoI YGGCCR 3 cut(s) 3, 207, 2535
AcsI RAATTY 7 cut(s) 224, 421, 753, 790, 1093, 1161, 1306
AcuI CTGAAG 1 cut(s) 2677
AcyI GRCGYC 1 cut(s) 2115
AfaI GTAC 5 cut(s) 824, 1050, 1745, 2475, 2677
AfiI CCNNNNNNNGG 4 cut(s) 1504, 2124, 2396, 2695
AflIII ACRYGT 1 cut(s) 2634
AhlI ACTAGT 1 cut(s) 1109
AjnI CCWGG 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 1084, 1116
AloI GAACNNNNNNTCC 2 cut(s) 1468, 1500
Alw21I GWGCWC 4 cut(s) 120, 736, 1997, 2711
Alw26I GTCTC 1 cut(s) 2171
Alw44I GTGCAC 1 cut(s) 2707
AlwNI CAGNNNCTG 2 cut(s) 1130, 2180
AoxI GGCC 4 cut(s) 3, 207, 1863, 2535
ApaLI GTGCAC 1 cut(s) 2707
ApeKI GCWGC 6 cut(s) 245, 664, 1903, 2183, 2512, 2624
ApoI RAATTY 7 cut(s) 224, 421, 753, 790, 1093, 1161, 1306
ArsI GACNNNNNNTTYG 2 cut(s) 2621, 2653
AseI ATTAAT 1 cut(s) 513
Asp700I GAANNNNTTC 3 cut(s) 336, 1700, 1979
AspLEI GCGC 2 cut(s) 709, 1772
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 6 cut(s) 125, 242, 646, 1679, 2323, 2678
AsuII TTCGAA 1 cut(s) 548
BaeGI GKGCMC 1 cut(s) 2711
BalI TGGCCA 3 cut(s) 5, 209, 2537
BanII GRGCYC 2 cut(s) 120, 1191
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 4 cut(s) 120, 736, 1997, 2711
BbvI GCAGC 6 cut(s) 232, 676, 1915, 2195, 2499, 2636
BccI CCATC 8 cut(s) 25, 624, 768, 1064, 1586, 1965, 2259, 2666
BceAI ACGGC 2 cut(s) 1048, 2477
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 3 cut(s) 465, 877, 2347
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 1 cut(s) 2171
BcuI ACTAGT 1 cut(s) 1109
BfaI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
BfmI CTRYAG 4 cut(s) 1458, 1901, 2181, 2622
BfoI RGCGCY 1 cut(s) 1773
BfuAI ACCTGC 1 cut(s) 2720
BisI GCNGC 7 cut(s) 246, 665, 1464, 1904, 2184, 2513, 2625
BlsI GCNGC 7 cut(s) 247, 666, 1465, 1905, 2185, 2514, 2626
BmcAI AGTACT 1 cut(s) 1050
Bme1390I CCNGG 2 cut(s) 221, 1081
BmiI GGNNCC 1 cut(s) 2208
BmrFI CCNGG 2 cut(s) 221, 1081
BmsI GCATC 7 cut(s) 931, 1066, 1339, 1900, 2088, 2479, 2499
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 2015, 2047
BpmI CTGGAG 1 cut(s) 811
Bpu14I TTCGAA 1 cut(s) 548
BpuEI CTTGAG 5 cut(s) 104, 326, 374, 1550, 2651
BpuMI CCSGG 1 cut(s) 1081
BsaBI GATNNNNATC 1 cut(s) 784
BsaHI GRCGYC 1 cut(s) 2115
BsaJI CCNNGG 1 cut(s) 284
BsaWI WCCGGW 1 cut(s) 1393
Bsc4I CCNNNNNNNGG 4 cut(s) 1504, 2124, 2396, 2695
Bse1I ACTGG 2 cut(s) 553, 2538
Bse3DI GCAATG 3 cut(s) 255, 1440, 2722
Bse8I GATNNNNATC 1 cut(s) 784
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 284
BseJI GATNNNNATC 1 cut(s) 784
BseLI CCNNNNNNNGG 4 cut(s) 1504, 2124, 2396, 2695
BseMI GCAATG 3 cut(s) 255, 1440, 2722
BseMII CTCAG 2 cut(s) 1965, 2507
BseNI ACTGG 2 cut(s) 553, 2538
BseSI GKGCMC 1 cut(s) 2711
BseXI GCAGC 6 cut(s) 232, 676, 1915, 2195, 2499, 2636
BsgI GTGCAG 3 cut(s) 255, 1662, 2391
BshFI GGCC 4 cut(s) 5, 209, 1865, 2537
BsiHKAI GWGCWC 4 cut(s) 120, 736, 1997, 2711
BsiSI CCGG 4 cut(s) 986, 1080, 1394, 2111
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 4 cut(s) 1504, 2124, 2396, 2695
BsmAI GTCTC 1 cut(s) 2171
BsmFI GGGAC 1 cut(s) 538
BsnI GGCC 4 cut(s) 5, 209, 1865, 2537
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 5 cut(s) 120, 736, 1191, 1997, 2711
Bsp1407I TGTACA 2 cut(s) 1743, 2473
Bsp143I GATC 3 cut(s) 465, 877, 2347
Bsp19I CCATGG 1 cut(s) 284
BspACI CCGC 2 cut(s) 1463, 2270
BspANI GGCC 4 cut(s) 5, 209, 1865, 2537
BspCNI CTCAG 2 cut(s) 1964, 2508
BspLI GGNNCC 1 cut(s) 2208
BspMAI CTGCAG 3 cut(s) 1905, 2185, 2626
BspMI ACCTGC 1 cut(s) 2720
BspT104I TTCGAA 1 cut(s) 548
BsrDI GCAATG 3 cut(s) 255, 1440, 2722
BsrGI TGTACA 2 cut(s) 1743, 2473
BsrI ACTGG 2 cut(s) 553, 2538
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 3 cut(s) 465, 877, 2347
BssNI GRCGYC 1 cut(s) 2115
BssT1I CCWWGG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 3 cut(s) 72, 1370, 1682
Bst6I CTCTTC 5 cut(s) 297, 916, 1032, 1058, 1665
BstACI GRCGYC 1 cut(s) 2115
BstAPI GCANNNNNTGC 4 cut(s) 149, 1610, 2180, 2721
BstAUI TGTACA 2 cut(s) 1743, 2473
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 456, 1756
BstDEI CTNAG 3 cut(s) 1399, 1951, 2516
BstDSI CCRYGG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 1773
BstHHI GCGC 2 cut(s) 709, 1772
BstKTI GATC 3 cut(s) 468, 880, 2350
BstMAI GTCTC 1 cut(s) 2171
BstMBI GATC 3 cut(s) 465, 877, 2347
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 3 cut(s) 458, 823, 2638
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 2 cut(s) 219, 1079
BstSFI CTRYAG 4 cut(s) 1458, 1901, 2181, 2622
BstSLI GKGCMC 1 cut(s) 2711
BstV1I GCAGC 6 cut(s) 232, 676, 1915, 2195, 2499, 2636
BstV2I GAAGAC 1 cut(s) 66
BsuRI GGCC 4 cut(s) 5, 209, 1865, 2537
BtgI CCRYGG 1 cut(s) 284
BtsIMutI CAGTG 3 cut(s) 141, 1678, 2646
BveI ACCTGC 1 cut(s) 2720
Cac8I GCNNGC 2 cut(s) 456, 1756
CaiI CAGNNNCTG 2 cut(s) 1130, 2180
CfoI GCGC 2 cut(s) 709, 1772
CseI GACGC 1 cut(s) 2123
Csp6I GTAC 5 cut(s) 823, 1049, 1744, 2474, 2676
CspCI CAANNNNNGTGG 2 cut(s) 1714, 1749
CviQI GTAC 5 cut(s) 823, 1049, 1744, 2474, 2676
DdeI CTNAG 3 cut(s) 1399, 1951, 2516
DpnI GATC 3 cut(s) 467, 879, 2349
DpnII GATC 3 cut(s) 465, 877, 2347
EaeI YGGCCR 3 cut(s) 3, 207, 2535
Eam1104I CTCTTC 5 cut(s) 297, 916, 1032, 1058, 1665
EarI CTCTTC 5 cut(s) 297, 916, 1032, 1058, 1665
EciI GGCGGA 1 cut(s) 2285
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 284
Eco24I GRGCYC 2 cut(s) 120, 1191
Eco32I GATATC 1 cut(s) 1317
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 2677
EcoICRI GAGCTC 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 1306
EcoRII CCWGG 1 cut(s) 219
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 1 cut(s) 284
EcoT22I ATGCAT 3 cut(s) 456, 573, 1171
EcoT38I GRGCYC 2 cut(s) 120, 1191
ErhI CCWWGG 1 cut(s) 284
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 1 cut(s) 538
FauNDI CATATG 1 cut(s) 2361
FbaI TGATCA 3 cut(s) 465, 877, 2347
Fnu4HI GCNGC 7 cut(s) 246, 665, 1464, 1904, 2184, 2513, 2625
FriOI GRGCYC 2 cut(s) 120, 1191
Fsp4HI GCNGC 7 cut(s) 246, 665, 1464, 1904, 2184, 2513, 2625
FspBI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
GlaI GCGC 2 cut(s) 708, 1771
GluI GCNGC 7 cut(s) 246, 665, 1464, 1904, 2184, 2513, 2625
GsuI CTGGAG 1 cut(s) 811
HaeII RGCGCY 1 cut(s) 1773
HaeIII GGCC 4 cut(s) 5, 209, 1865, 2537
HapII CCGG 4 cut(s) 986, 1080, 1394, 2111
HgaI GACGC 1 cut(s) 2123
HhaI GCGC 2 cut(s) 709, 1772
Hin1I GRCGYC 1 cut(s) 2115
Hin6I GCGC 2 cut(s) 707, 1770
HinP1I GCGC 2 cut(s) 707, 1770
HincII GTYRAC 1 cut(s) 1494
HindII GTYRAC 1 cut(s) 1494
HindIII AAGCTT 3 cut(s) 714, 1635, 2076
HinfI GANTC 9 cut(s) 233, 785, 866, 1375, 1386, 1549, 1559, 2191, 2518
HpaI GTTAAC 1 cut(s) 1494
HpaII CCGG 4 cut(s) 986, 1080, 1394, 2111
HphI GGTGA 6 cut(s) 125, 242, 646, 1679, 2323, 2678
Hpy166II GTNNAC 5 cut(s) 955, 1494, 1538, 2474, 2709
Hpy188I TCNGA 7 cut(s) 976, 1132, 1476, 1766, 2352, 2434, 2611
Hpy8I GTNNAC 5 cut(s) 955, 1494, 1538, 2474, 2709
HpyCH4III ACNGT 3 cut(s) 72, 1370, 1682
HpyCH4IV ACGT 1 cut(s) 1503
HpyF3I CTNAG 3 cut(s) 1399, 1951, 2516
HpySE526I ACGT 1 cut(s) 1503
Hsp92I GRCGYC 1 cut(s) 2115
HspAI GCGC 2 cut(s) 707, 1770
Ksp22I TGATCA 3 cut(s) 465, 877, 2347
KspAI GTTAAC 1 cut(s) 1494
Kzo9I GATC 3 cut(s) 465, 877, 2347
LmnI GCTCC 5 cut(s) 19, 50, 1892, 2017, 2331
Lsp1109I GCAGC 6 cut(s) 232, 676, 1915, 2195, 2499, 2636
LweI GCATC 7 cut(s) 931, 1066, 1339, 1900, 2088, 2479, 2499
MaeI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
MaeII ACGT 1 cut(s) 1503
MaeIII GTNAC 5 cut(s) 215, 994, 1402, 1682, 2519
MalI GATC 3 cut(s) 467, 879, 2349
MboI GATC 3 cut(s) 465, 877, 2347
MhlI GDGCHC 5 cut(s) 120, 736, 1191, 1997, 2711
MlsI TGGCCA 3 cut(s) 5, 209, 2537
MluNI TGGCCA 3 cut(s) 5, 209, 2537
MlyI GAGTC 2 cut(s) 1384, 2527
MmeI TCCRAC 5 cut(s) 72, 195, 426, 2039, 2184
Mox20I TGGCCA 3 cut(s) 5, 209, 2537
Mph1103I ATGCAT 3 cut(s) 456, 573, 1171
MroXI GAANNNNTTC 3 cut(s) 336, 1700, 1979
MscI TGGCCA 3 cut(s) 5, 209, 2537
MslI CAYNNNNRTG 3 cut(s) 762, 1829, 2639
Msp20I TGGCCA 3 cut(s) 5, 209, 2537
MspA1I CMGCKG 2 cut(s) 245, 667
MspI CCGG 4 cut(s) 986, 1080, 1394, 2111
MspR9I CCNGG 2 cut(s) 221, 1081
MvaI CCWGG 1 cut(s) 221
NciI CCSGG 1 cut(s) 1081
NcoI CCATGG 1 cut(s) 284
NdeI CATATG 1 cut(s) 2361
NdeII GATC 3 cut(s) 465, 877, 2347
NlaIV GGNNCC 1 cut(s) 2208
NmuCI GTSAC 4 cut(s) 994, 1402, 1682, 2519
NsiI ATGCAT 3 cut(s) 456, 573, 1171
NspI RCATGY 3 cut(s) 458, 823, 2638
NspV TTCGAA 1 cut(s) 548
PaeI GCATGC 1 cut(s) 458
PaqCI CACCTGC 1 cut(s) 2720
PciI ACATGT 1 cut(s) 2634
PdmI GAANNNNTTC 3 cut(s) 336, 1700, 1979
PfeI GAWTC 7 cut(s) 233, 785, 866, 1386, 1549, 1559, 2191
PkrI GCNGC 7 cut(s) 247, 666, 1465, 1905, 2185, 2514, 2626
PleI GAGTC 2 cut(s) 1383, 2526
PpsI GAGTC 2 cut(s) 1383, 2526
PscI ACATGT 1 cut(s) 2634
PshAI GACNNNNGTC 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 513
PsiI TTATAA 1 cut(s) 918
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 2208
PstI CTGCAG 3 cut(s) 1905, 2185, 2626
PstNI CAGNNNCTG 2 cut(s) 1130, 2180
PvuII CAGCTG 2 cut(s) 245, 667
RsaI GTAC 5 cut(s) 824, 1050, 1745, 2475, 2677
RsaNI GTAC 5 cut(s) 823, 1049, 1744, 2474, 2676
RseI CAYNNNNRTG 3 cut(s) 762, 1829, 2639
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 7 cut(s) 246, 665, 1464, 1904, 2184, 2513, 2625
Sau3AI GATC 3 cut(s) 465, 877, 2347
ScaI AGTACT 1 cut(s) 1050
SchI GAGTC 2 cut(s) 1384, 2527
ScrFI CCNGG 2 cut(s) 221, 1081
SduI GDGCHC 5 cut(s) 120, 736, 1191, 1997, 2711
SfaNI GCATC 7 cut(s) 931, 1066, 1339, 1900, 2088, 2479, 2499
SfcI CTRYAG 4 cut(s) 1458, 1901, 2181, 2622
SfuI TTCGAA 1 cut(s) 548
SmiMI CAYNNNNRTG 3 cut(s) 762, 1829, 2639
SmlI CTYRAG 5 cut(s) 119, 341, 353, 1565, 2666
SmoI CTYRAG 5 cut(s) 119, 341, 353, 1565, 2666
SpeI ACTAGT 1 cut(s) 1109
SphI GCATGC 1 cut(s) 458
SsiI CCGC 2 cut(s) 1463, 2270
SspMI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 219, 1079
StyI CCWWGG 1 cut(s) 284
TaaI ACNGT 3 cut(s) 72, 1370, 1682
TaiI ACGT 1 cut(s) 1506
TaqI TCGA 8 cut(s) 548, 788, 902, 1191, 1737, 1962, 1983, 2277
TatI WGTACW 4 cut(s) 822, 1048, 1743, 2473
TauI GCSGC 1 cut(s) 1466
TfiI GAWTC 7 cut(s) 233, 785, 866, 1386, 1549, 1559, 2191
TscAI CASTG 3 cut(s) 141, 1685, 2646
TseFI GTSAC 4 cut(s) 994, 1402, 1682, 2519
TseI GCWGC 6 cut(s) 245, 664, 1903, 2183, 2512, 2624
Tsp45I GTSAC 4 cut(s) 994, 1402, 1682, 2519
TspGWI ACGGA 2 cut(s) 1708, 1905
TspRI CASTG 3 cut(s) 141, 1685, 2646
VneI GTGCAC 1 cut(s) 2707
VspI ATTAAT 1 cut(s) 513
XapI RAATTY 7 cut(s) 224, 421, 753, 790, 1093, 1161, 1306
XbaI TCTAGA 2 cut(s) 298, 643
XceI RCATGY 3 cut(s) 458, 823, 2638
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XmnI GAANNNNTTC 3 cut(s) 336, 1700, 1979
XspI CTAG 7 cut(s) 11, 66, 237, 299, 644, 842, 1110
ZrmI AGTACT 1 cut(s) 1050
Zsp2I ATGCAT 3 cut(s) 456, 573, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.