Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0014938

Physical Location & Seq

Genomic Coordinates Reverse (-)

25161633 .. 25162610

Gene Structure 978 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00304520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGATCCGGTTGCAGCACACGGCCGACCACTTCCGCCTCCGTTTTTGACGAGAGATCTTCATCTAAATCACCCTCATCATCAGTTCCAACACCACCCTCACCTCCTCCACCACCAGAATTCCGAAGACGAGCAGAACAGCTCCGGCTTAAACCACCTCCGGGGAATCAAGCGCGACCGGGACGAGAACACCTCCGGCGCCACCACCACGAACTCGCTGGAAGGCAAGGACCAGCTTGGTGGGTCCACTAGCGGAGAAGGCGGCGAGATCACGAGACGACCTCGTGGCCGACCCGCTGGCTCCAAGAACAAGGCCAAACCCCCAATCATCATCACGCGCGACAGCGCCAACGCGTTGCGGTCTCACGTCATGGAGGTCGCCAACGGCTGCGACATCATGGACTCCATCTCCACCTTCGCCCGCCGGAGACAAAGAGGCGTTTGCATTCTCAGCGGCAGCGGCACCGTCACCAATGTAACCCTCCGCCAGCCAGCTTCGCCCGGCGCCGTGGTCACCCTCCACGGTAGGTTCGAAATTCTTTCCCTTTCAGGATCATTCCTTCCCCCACCTGCTCCGCCCGCCGCGTCAGGACTCACCATTTACCTGGCTGGCGGGCAGGGACAGGTAGTCGGAGGCAGCGTTGTGGGCCCGCTCCTAGCGTCCGGCCCTGTGGTCATTATGGCAGCGAGCTTCGGTAATGCTGCCTACGAGAGACTGCCCCTGGAGGAGGAGGAGGCCACGGCGGTGACGCCTATGCCTGGGAGCGGGCCCCTTGGCTCCCCTGGGCTTGGCGGGGGAGAGCAGCAGCAACAGTCACAAGTTCAACAAGTACAGCAGCAGCAGCAGCAGCAGATGTTGCAAGATGCTAATAACTCATCACCTTCTTCCTCACTCTTTCATGGGATGCAGCAAAATCTTCTAAATTCGGTTCAATTGCCCGCTGAGGCATATTGGGGCACAGCTCGTCCTCCTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003680 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009642 GO:0009646 GO:0009647 GO:0009791 GO:0009889 GO:0010228 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019899 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043621 GO:0044424 GO:0044464 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF296

Protein Analysis

325

Amino Acids

34.42

Weight (kDa)

6.93

Isoelectric Point (pI)

55.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCC	PF03479	120 - 231	6.6e-25	Plants and Prokaryotes Conserved (PCC) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014938)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45430 AT3G60870
fragaria_vesca	FvH4_7g12040
malus_domestica	MD02G1201800.v1.1
prunus_persica	Prupe.2G147000_v2.0.a1
pyrus_communis	pycom07g11310
rosa_chinensis	RchiOBHm_Chr1g0350751
rosa_laevigata	RLG00000028456
rosa_multiflora	Rmu_sc0007810.1_g000008
rosa_roxburghii	Rroxscaffold_4G00304520
rosa_rugosa	Rorug01G0213900
rosa_samantha	Rh1AG228000 Rh1BG197500 Rh1CG213000 Rh1DG225400
rosa_wichuraiana	Rw1G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	577
Acc36I	ACCTGC	1 cut(s)	577
AccB1I	GGYRCC	3 cut(s)	197, 461, 503
AccBSI	CCGCTC	2 cut(s)	652, 765
AccII	CGCG	5 cut(s)	174, 337, 339, 353, 584
AclWI	GGATC	2 cut(s)	12, 559
AcoI	YGGCCR	2 cut(s)	22, 286
AcsI	RAATTY	3 cut(s)	118, 534, 922
AcyI	GRCGYC	3 cut(s)	198, 504, 749
AfaI	GTAC	1 cut(s)	831
AfiI	CCNNNNNNNGG	5 cut(s)	160, 727, 758, 764, 788
AflIII	ACRYGT	1 cut(s)	351
AgsI	TTSAA	2 cut(s)	824, 932
AhdI	GACNNNNNGTC	1 cut(s)	626
AjiI	CACGTC	1 cut(s)	367
AjnI	CCWGG	4 cut(s)	603, 720, 757, 781
AleI	CACNNNNGTG	1 cut(s)	743
AluBI	AGCT	5 cut(s)	141, 235, 494, 690, 962
AluI	AGCT	5 cut(s)	141, 235, 494, 690, 962
Alw26I	GTCTC	4 cut(s)	268, 366, 421, 706
AlwI	GGATC	2 cut(s)	12, 559
AoxI	GGCC	7 cut(s)	22, 286, 312, 646, 664, 735, 767
ApaI	GGGCCC	2 cut(s)	650, 771
ApoI	RAATTY	3 cut(s)	118, 534, 922
AspLEI	GCGC	5 cut(s)	174, 200, 339, 347, 506
AspS9I	GGNCC	7 cut(s)	229, 243, 646, 647, 665, 767, 768
AsuC2I	CCSGG	3 cut(s)	161, 179, 501
AsuHPI	GGTGA	7 cut(s)	62, 92, 460, 505, 586, 757, 870
AsuII	TTCGAA	1 cut(s)	531
AvaII	GGWCC	2 cut(s)	229, 243
BaeGI	GKGCMC	3 cut(s)	650, 771, 959
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	3 cut(s)	197, 461, 503
BanII	GRGCYC	2 cut(s)	650, 771
BauI	CACGAG	2 cut(s)	271, 282
BbsI	GAAGAC	1 cut(s)	132
BbvCI	CCTCAGC	1 cut(s)	942
BccI	CCATC	1 cut(s)	413
BceAI	ACGGC	4 cut(s)	37, 400, 491, 756
BciT130I	CCWGG	4 cut(s)	605, 722, 759, 783
BcnI	CCSGG	3 cut(s)	161, 179, 501
BcoDI	GTCTC	4 cut(s)	268, 366, 421, 706
BfaI	CTAG	2 cut(s)	249, 656
BfoI	RGCGCY	3 cut(s)	201, 348, 507
BfuAI	ACCTGC	1 cut(s)	577
BglII	AGATCT	1 cut(s)	55
Bme1390I	CCNGG	7 cut(s)	161, 179, 501, 605, 722, 759, 783
Bme18I	GGWCC	2 cut(s)	229, 243
BmeRI	GACNNNNNGTC	1 cut(s)	626
BmgBI	CACGTC	1 cut(s)	367
BmgT120I	GGNCC	7 cut(s)	229, 243, 646, 647, 665, 767, 768
BmrFI	CCNGG	7 cut(s)	161, 179, 501, 605, 722, 759, 783
BmsI	GCATC	2 cut(s)	853, 894
BpiI	GAAGAC	1 cut(s)	132
BplI	GAGNNNNNCTC	4 cut(s)	176, 208, 265, 297
BpmI	CTGGAG	1 cut(s)	743
Bpu10I	CCTNAGC	1 cut(s)	942
Bpu14I	TTCGAA	1 cut(s)	531
BpuMI	CCSGG	3 cut(s)	161, 179, 501
BsaBI	GATNNNNATC	1 cut(s)	60
BsaHI	GRCGYC	3 cut(s)	198, 504, 749
BsaI	GGTCTC	1 cut(s)	366
BsaJI	CCNNGG	9 cut(s)	160, 507, 520, 720, 738, 758, 772, 781, 782
BsaWI	WCCGGW	1 cut(s)	7
BsaXI	ACNNNNNCTCC	4 cut(s)	392, 422, 624, 654
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 727, 758, 764, 788
Bse8I	GATNNNNATC	1 cut(s)	60
BseBI	CCWGG	4 cut(s)	605, 722, 759, 783
BseDI	CCNNGG	9 cut(s)	160, 507, 520, 720, 738, 758, 772, 781, 782
BseGI	GGATG	1 cut(s)	909
BseJI	GATNNNNATC	1 cut(s)	60
BseLI	CCNNNNNNNGG	5 cut(s)	160, 727, 758, 764, 788
BseMII	CTCAG	2 cut(s)	463, 933
BseRI	GAGGAG	4 cut(s)	95, 740, 743, 746
BseSI	GKGCMC	3 cut(s)	650, 771, 959
BseX3I	CGGCCG	1 cut(s)	22
Bsh1236I	CGCG	5 cut(s)	174, 337, 339, 353, 584
Bsh1285I	CGRYCG	2 cut(s)	25, 178
BshFI	GGCC	7 cut(s)	24, 288, 314, 648, 666, 737, 769
BshNI	GGYRCC	3 cut(s)	197, 461, 503
BsiEI	CGRYCG	2 cut(s)	25, 178
BsiSI	CCGG	8 cut(s)	8, 144, 160, 178, 195, 424, 501, 663
BslFI	GGGAC	2 cut(s)	194, 633
BslI	CCNNNNNNNGG	5 cut(s)	160, 727, 758, 764, 788
BsmAI	GTCTC	4 cut(s)	268, 366, 421, 706
BsmBI	CGTCTC	1 cut(s)	268
BsmFI	GGGAC	2 cut(s)	194, 633
BsmI	GAATGC	1 cut(s)	444
BsnI	GGCC	7 cut(s)	24, 288, 314, 648, 666, 737, 769
Bso31I	GGTCTC	1 cut(s)	366
Bsp119I	TTCGAA	1 cut(s)	531
Bsp120I	GGGCCC	2 cut(s)	646, 767
Bsp1286I	GDGCHC	3 cut(s)	650, 771, 959
Bsp143I	GATC	4 cut(s)	4, 55, 267, 551
BspANI	GGCC	7 cut(s)	24, 288, 314, 648, 666, 737, 769
BspCNI	CTCAG	2 cut(s)	462, 934
BspFNI	CGCG	5 cut(s)	174, 337, 339, 353, 584
BspMI	ACCTGC	1 cut(s)	577
BspPI	GGATC	2 cut(s)	12, 559
BspT104I	TTCGAA	1 cut(s)	531
BspT107I	GGYRCC	3 cut(s)	197, 461, 503
BspTNI	GGTCTC	1 cut(s)	366
BsrBI	CCGCTC	2 cut(s)	652, 765
BssECI	CCNNGG	9 cut(s)	160, 507, 520, 720, 738, 758, 772, 781, 782
BssMI	GATC	4 cut(s)	4, 55, 267, 551
BssNI	GRCGYC	3 cut(s)	198, 504, 749
BssSI	CACGAG	2 cut(s)	271, 282
BssT1I	CCWWGG	1 cut(s)	772
Bst2BI	CACGAG	2 cut(s)	271, 282
Bst2UI	CCWGG	4 cut(s)	605, 722, 759, 783
Bst4CI	ACNGT	3 cut(s)	466, 524, 813
BstACI	GRCGYC	3 cut(s)	198, 504, 749
BstAPI	GCANNNNNTGC	1 cut(s)	856
BstBI	TTCGAA	1 cut(s)	531
BstDEI	CTNAG	2 cut(s)	449, 942
BstDSI	CCRYGG	3 cut(s)	507, 520, 738
BstEII	GGTNACC	1 cut(s)	511
BstENI	CCTNNNNNAGG	1 cut(s)	725
BstF5I	GGATG	1 cut(s)	909
BstFNI	CGCG	5 cut(s)	174, 337, 339, 353, 584
BstH2I	RGCGCY	3 cut(s)	201, 348, 507
BstHHI	GCGC	5 cut(s)	174, 200, 339, 347, 506
BstKTI	GATC	4 cut(s)	7, 58, 270, 554
BstMAI	GTCTC	4 cut(s)	268, 366, 421, 706
BstMBI	GATC	4 cut(s)	4, 55, 267, 551
BstMCI	CGRYCG	2 cut(s)	25, 178
BstNI	CCWGG	4 cut(s)	605, 722, 759, 783
BstPI	GGTNACC	1 cut(s)	511
BstSCI	CCNGG	7 cut(s)	159, 177, 499, 603, 720, 757, 781
BstSLI	GKGCMC	3 cut(s)	650, 771, 959
BstUI	CGCG	5 cut(s)	174, 337, 339, 353, 584
BstV2I	GAAGAC	1 cut(s)	132
BstX2I	RGATCY	2 cut(s)	4, 55
BstXI	CCANNNNNNTGG	2 cut(s)	239, 604
BstYI	RGATCY	2 cut(s)	4, 55
BstZI	CGGCCG	1 cut(s)	22
BsuRI	GGCC	7 cut(s)	24, 288, 314, 648, 666, 737, 769
BtgI	CCRYGG	3 cut(s)	507, 520, 738
BtrI	CACGTC	1 cut(s)	367
BtsCI	GGATG	1 cut(s)	909
BveI	ACCTGC	1 cut(s)	577
CfoI	GCGC	5 cut(s)	174, 200, 339, 347, 506
Cfr13I	GGNCC	7 cut(s)	229, 243, 646, 647, 665, 767, 768
CseI	GACGC	3 cut(s)	573, 648, 757
Csp6I	GTAC	1 cut(s)	830
CviAII	CATG	3 cut(s)	370, 397, 899
CviQI	GTAC	1 cut(s)	830
DdeI	CTNAG	2 cut(s)	449, 942
DinI	GGCGCC	2 cut(s)	199, 505
DpnI	GATC	4 cut(s)	6, 57, 269, 553
DpnII	GATC	4 cut(s)	4, 55, 267, 551
DriI	GACNNNNNGTC	1 cut(s)	626
EaeI	YGGCCR	2 cut(s)	22, 286
EagI	CGGCCG	1 cut(s)	22
Eam1105I	GACNNNNNGTC	1 cut(s)	626
EciI	GGCGGA	3 cut(s)	24, 473, 564
EclXI	CGGCCG	1 cut(s)	22
Eco130I	CCWWGG	1 cut(s)	772
Eco24I	GRGCYC	2 cut(s)	650, 771
Eco31I	GGTCTC	1 cut(s)	366
Eco47I	GGWCC	2 cut(s)	229, 243
Eco52I	CGGCCG	1 cut(s)	22
Eco91I	GGTNACC	1 cut(s)	511
EcoNI	CCTNNNNNAGG	1 cut(s)	725
EcoO109I	RGGNCCY	1 cut(s)	768
EcoO65I	GGTNACC	1 cut(s)	511
EcoRI	GAATTC	1 cut(s)	118
EcoRII	CCWGG	4 cut(s)	603, 720, 757, 781
EcoT14I	CCWWGG	1 cut(s)	772
EcoT38I	GRGCYC	2 cut(s)	650, 771
EgeI	GGCGCC	2 cut(s)	199, 505
EheI	GGCGCC	2 cut(s)	199, 505
ErhI	CCWWGG	1 cut(s)	772
Esp3I	CGTCTC	1 cut(s)	268
FaeI	CATG	3 cut(s)	373, 400, 902
FaiI	YATR	6 cut(s)	371, 398, 680, 755, 900, 949
FaqI	GGGAC	2 cut(s)	194, 633
FatI	CATG	3 cut(s)	369, 396, 898
FauI	CCCGC	8 cut(s)	301, 428, 586, 605, 657, 758, 785, 946
FokI	GGATG	1 cut(s)	916
FriOI	GRGCYC	2 cut(s)	650, 771
FspBI	CTAG	2 cut(s)	249, 656
GlaI	GCGC	5 cut(s)	173, 199, 338, 346, 505
GsuI	CTGGAG	1 cut(s)	743
HaeII	RGCGCY	3 cut(s)	201, 348, 507
HaeIII	GGCC	7 cut(s)	24, 288, 314, 648, 666, 737, 769
HapII	CCGG	8 cut(s)	8, 144, 160, 178, 195, 424, 501, 663
HgaI	GACGC	3 cut(s)	573, 648, 757
HhaI	GCGC	5 cut(s)	174, 200, 339, 347, 506
Hin1I	GRCGYC	3 cut(s)	198, 504, 749
Hin1II	CATG	3 cut(s)	373, 400, 902
Hin6I	GCGC	5 cut(s)	172, 198, 337, 345, 504
HinP1I	GCGC	5 cut(s)	172, 198, 337, 345, 504
HinfI	GANTC	3 cut(s)	165, 401, 591
HpaII	CCGG	8 cut(s)	8, 144, 160, 178, 195, 424, 501, 663
HphI	GGTGA	7 cut(s)	62, 92, 460, 505, 586, 757, 870
Hpy166II	GTNNAC	1 cut(s)	246
Hpy188I	TCNGA	2 cut(s)	124, 632
Hpy188III	TCNNGA	3 cut(s)	271, 549, 588
Hpy8I	GTNNAC	1 cut(s)	246
HpyAV	CCTTC	5 cut(s)	215, 251, 424, 569, 891
HpyCH4III	ACNGT	3 cut(s)	466, 524, 813
HpyCH4IV	ACGT	1 cut(s)	366
HpyCH4V	TGCA	4 cut(s)	14, 444, 859, 907
HpyF3I	CTNAG	2 cut(s)	449, 942
HpySE526I	ACGT	1 cut(s)	366
Hsp92I	GRCGYC	3 cut(s)	198, 504, 749
Hsp92II	CATG	3 cut(s)	373, 400, 902
HspAI	GCGC	5 cut(s)	172, 198, 337, 345, 504
KasI	GGCGCC	2 cut(s)	197, 503
Kzo9I	GATC	4 cut(s)	4, 55, 267, 551
LmnI	GCTCC	6 cut(s)	146, 305, 577, 657, 762, 782
LweI	GCATC	2 cut(s)	853, 894
MaeI	CTAG	2 cut(s)	249, 656
MaeII	ACGT	1 cut(s)	366
MaeIII	GTNAC	5 cut(s)	466, 475, 511, 745, 813
MalI	GATC	4 cut(s)	6, 57, 269, 553
MbiI	CCGCTC	2 cut(s)	652, 765
MboI	GATC	4 cut(s)	4, 55, 267, 551
MboII	GAAGA	4 cut(s)	50, 137, 876, 908
MfeI	CAATTG	1 cut(s)	932
MflI	RGATCY	2 cut(s)	4, 55
MhlI	GDGCHC	3 cut(s)	650, 771, 959
MluCI	AATT	4 cut(s)	118, 534, 922, 932
MluI	ACGCGT	1 cut(s)	351
Mly113I	GGCGCC	2 cut(s)	198, 504
MlyI	GAGTC	2 cut(s)	395, 585
MmeI	TCCRAC	2 cut(s)	112, 610
MseI	TTAA	2 cut(s)	149, 976
MslI	CAYNNNNRTG	1 cut(s)	743
MspA1I	CMGCKG	4 cut(s)	296, 453, 459, 941
MspI	CCGG	8 cut(s)	8, 144, 160, 178, 195, 424, 501, 663
MspR9I	CCNGG	7 cut(s)	161, 179, 501, 605, 722, 759, 783
MunI	CAATTG	1 cut(s)	932
Mva1269I	GAATGC	1 cut(s)	444
MvaI	CCWGG	4 cut(s)	605, 722, 759, 783
MvnI	CGCG	5 cut(s)	174, 337, 339, 353, 584
NarI	GGCGCC	2 cut(s)	198, 504
NciI	CCSGG	3 cut(s)	161, 179, 501
NdeII	GATC	4 cut(s)	4, 55, 267, 551
NlaIII	CATG	3 cut(s)	373, 400, 902
NmuCI	GTSAC	4 cut(s)	466, 511, 745, 813
NspV	TTCGAA	1 cut(s)	531
OliI	CACNNNNGTG	1 cut(s)	743
PaqCI	CACCTGC	1 cut(s)	577
PasI	CCCWGGG	1 cut(s)	782
PcsI	WCGNNNNNNNCGW	2 cut(s)	528, 636
PctI	GAATGC	1 cut(s)	444
PfeI	GAWTC	1 cut(s)	165
PleI	GAGTC	2 cut(s)	395, 585
PluTI	GGCGCC	2 cut(s)	201, 507
PpsI	GAGTC	2 cut(s)	395, 585
Psp6I	CCWGG	4 cut(s)	603, 720, 757, 781
PspEI	GGTNACC	1 cut(s)	511
PspGI	CCWGG	4 cut(s)	603, 720, 757, 781
PspOMI	GGGCCC	2 cut(s)	646, 767
PspPI	GGNCC	7 cut(s)	229, 243, 646, 647, 665, 767, 768
PsuI	RGATCY	2 cut(s)	4, 55
RsaI	GTAC	1 cut(s)	831
RsaNI	GTAC	1 cut(s)	830
RseI	CAYNNNNRTG	1 cut(s)	743
SaqAI	TTAA	2 cut(s)	149, 976
Sau3AI	GATC	4 cut(s)	4, 55, 267, 551
Sau96I	GGNCC	7 cut(s)	229, 243, 646, 647, 665, 767, 768
SchI	GAGTC	2 cut(s)	395, 585
ScrFI	CCNGG	7 cut(s)	161, 179, 501, 605, 722, 759, 783
SduI	GDGCHC	3 cut(s)	650, 771, 959
SfaNI	GCATC	2 cut(s)	853, 894
SfoI	GGCGCC	2 cut(s)	199, 505
SfuI	TTCGAA	1 cut(s)	531
SinI	GGWCC	2 cut(s)	229, 243
SmiMI	CAYNNNNRTG	1 cut(s)	743
Sse9I	AATT	4 cut(s)	118, 534, 922, 932
SspDI	GGCGCC	2 cut(s)	197, 503
SspMI	CTAG	2 cut(s)	249, 656
StyD4I	CCNGG	7 cut(s)	159, 177, 499, 603, 720, 757, 781
StyI	CCWWGG	1 cut(s)	772
TaaI	ACNGT	3 cut(s)	466, 524, 813
TaiI	ACGT	1 cut(s)	369
TaqI	TCGA	1 cut(s)	531
TasI	AATT	4 cut(s)	118, 534, 922, 932
TatI	WGTACW	1 cut(s)	829
TauI	GCSGC	4 cut(s)	264, 456, 462, 584
TfiI	GAWTC	1 cut(s)	165
Tru1I	TTAA	2 cut(s)	149, 976
Tru9I	TTAA	2 cut(s)	149, 976
TseFI	GTSAC	4 cut(s)	466, 511, 745, 813
Tsp45I	GTSAC	4 cut(s)	466, 511, 745, 813
TspDTI	ATGAA	2 cut(s)	50, 887
TspGWI	ACGGA	1 cut(s)	30
VpaK11BI	GGWCC	2 cut(s)	229, 243
XagI	CCTNNNNNAGG	1 cut(s)	725
XapI	RAATTY	3 cut(s)	118, 534, 922
XcmI	CCANNNNNNNNNTGG	1 cut(s)	214
XspI	CTAG	2 cut(s)	249, 656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.