Rroxscaffold_4G00320710

Cysteine desulfurase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006305
Physical Location & Seq
Forward (+)
49570744 .. 49576835
6092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320710.1

Sequence Viewer

Length: 1377 bp
ATGACACTGGAAGGAGTGGCCTTAAAATTGCCCCCATATTCATTCCCCATGATAACAACCCCTGCCTGCCGCACCCCTCACACCTTCCGTCGCTTTCTCGTCTCGGCTTCAACTGCTGCAGCTCCACCCGCATCCCTCTCACTTGGTCACTCGACGCGACCCGATTTCCCTATCCTTCACCAGGAAGTAAATGGCTCTAGACTTGTTTACTTGGACAATGCTGCAACTTCCCAGAAGCCTGTCACAGTGTTAAAGGCTTTGCAGGATTACTATCAAGGTTACAATTCAAACGTGCATCGCGGGATTCATTACTTAAGTTCAAAGGCAACAATGGAATATGAATTGGCGAGAAAGAAGATTTCGGAATTTATCAATGCATCAGATGCTCGAGAAATTGTTTTTACTAGGAATGCTACTGAAGCTATCAATCTGGTTGCTCACTCGTGGGGACTCCAAAATATAAAATCAAATGATGAGATCTTACTCACAATTGCCGAACATCACAGTGCGATTGTTCCCTGGCAATTTGTAGCTCAAAAGACCGGTGCCATTTTGAAATACGTGGAACTAAATGAAGATGGAGTTCCAGATGTGGAAAAGTTAAAAGATATGCTTTCAAGAAAGACAAAACTTGTAGTTGTTCATCATGTCTCAAATGTGCTAGGTTCTGTTCTTCCTATTGAAGATATCACGTGTTGTGCTCATGATGTTGGGGCAAAAGTTCTTGTAGATGCTTGTCAGAGTGTTCCACACATGGCGGTTGATGTACAGGATCTTAATGCTGACTTTCTTGTTGCTTCCTCTCACAAGATGTGTGGGCCTACGGGAATTGGATTCTTATACGGCAAGAGTGACCTATTTTCTTCCATGCCTCCATTTTTAGGTGGTGGAGAAATGATATCTGATGTATTTCTTGATCATTCCACTTATGCCGAACCTCCCTCTAGATTCGAGGCTGGAACACCAGCAATTGGGGAAGCAATTGGGCTAGGAGCAGCTATTGATTATTTGTCTGGAATTGGTATGCAACGGATACATGAATATGAGATAGAGCTGGCCAAATATCTATATGATAGTCTTTGTTCAATACCCAACATTCGAATTTATGGTCCGGTGCCTTCAGAAGATGTCCACCGCGCTGCTCTTTGTTCTTTCAATGTCGAGAATATACATCCTACAGATTTGGCAACTCTTCTTGACCAACAGCATGGAGTGGCTATCCGATCAGGTCACCATTGCGCCCAACCCCTTCATCGACATTTAGGCGTCGGTGCAAGTGCACGTGCGAGTCTTTACTTCTACAACACTAGAGAGGATGTTGATGACTTTATCCATGCCCTAAATGACACAGTCAGATTCTTTAATTCTTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00450 ko01100 map00450 map01100
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

458

Amino Acids

50.32

Weight (kDa)

6.0

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 69 - 443 3.2e-147 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 122 - 250 7.7e-07 DegT/DnrJ/EryC1/StrS aminotransferase family
Cys_Met_Meta_PP PF01053 154 - 270 1.6e-06 Cys/Met metabolism PLP-dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 545, 1114
AccB7I CCANNNNNTGG 1 cut(s) 971
AccII CGCG 3 cut(s) 157, 300, 1137
AciI CCGC 5 cut(s) 70, 129, 300, 758, 1135
AclWI GGATC 1 cut(s) 780
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 2 cut(s) 365, 1101
AcuI CTGAAG 2 cut(s) 438, 1104
AcvI CACGTG 2 cut(s) 693, 1283
AcyI GRCGYC 1 cut(s) 1266
AfaI GTAC 1 cut(s) 768
AfiI CCNNNNNNNGG 3 cut(s) 181, 881, 971
AflII CTTAAG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 692
AgeI ACCGGT 1 cut(s) 542
AgsI TTSAA 9 cut(s) 111, 288, 321, 556, 618, 683, 1086, 1156, 1372
AjnI CCWGG 2 cut(s) 180, 518
AluBI AGCT 5 cut(s) 122, 422, 533, 998, 1054
AluI AGCT 5 cut(s) 122, 422, 533, 998, 1054
Alw21I GWGCWC 2 cut(s) 703, 1282
Alw26I GTCTC 2 cut(s) 106, 655
Alw44I GTGCAC 1 cut(s) 1278
AlwI GGATC 1 cut(s) 780
Ama87I CYCGRG 1 cut(s) 387
AoxI GGCC 3 cut(s) 18, 818, 1056
ApaLI GTGCAC 1 cut(s) 1278
ApeKI GCWGC 5 cut(s) 116, 119, 221, 995, 1139
ApoI RAATTY 2 cut(s) 365, 1101
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
AsiGI ACCGGT 1 cut(s) 542
AspLEI GCGC 2 cut(s) 1139, 1241
AspS9I GGNCC 2 cut(s) 818, 1109
AsuHPI GGTGA 2 cut(s) 170, 1223
AsuII TTCGAA 1 cut(s) 1099
AvaI CYCGRG 1 cut(s) 387
AvaII GGWCC 1 cut(s) 1109
BaeGI GKGCMC 1 cut(s) 1282
BalI TGGCCA 1 cut(s) 1058
BanI GGYRCC 2 cut(s) 545, 1114
BauI CACGAG 1 cut(s) 442
BbrPI CACGTG 2 cut(s) 693, 1283
Bbv12I GWGCWC 2 cut(s) 703, 1282
BbvI GCAGC 5 cut(s) 103, 131, 208, 1007, 1126
BccI CCATC 1 cut(s) 572
BceAI ACGGC 1 cut(s) 859
BciT130I CCWGG 2 cut(s) 182, 520
BciVI GTATCC 1 cut(s) 1026
BclI TGATCA 1 cut(s) 916
BcoDI GTCTC 2 cut(s) 106, 655
BfaI CTAG 6 cut(s) 198, 405, 662, 945, 989, 1308
BfmI CTRYAG 2 cut(s) 117, 1176
BfrI CTTAAG 1 cut(s) 313
BfuI GTATCC 1 cut(s) 1026
BglII AGATCT 1 cut(s) 477
BisI GCNGC 6 cut(s) 70, 117, 120, 222, 996, 1140
BlsI GCNGC 6 cut(s) 71, 118, 121, 223, 997, 1141
Bme1390I CCNGG 2 cut(s) 182, 520
Bme18I GGWCC 1 cut(s) 1109
BmeT110I CYCGRG 1 cut(s) 387
BmgT120I GGNCC 2 cut(s) 818, 1109
BmiI GGNNCC 2 cut(s) 547, 1116
BmrFI CCNGG 2 cut(s) 182, 520
BmsI GCATC 5 cut(s) 140, 304, 373, 386, 721
Bpu14I TTCGAA 1 cut(s) 1099
BsaAI YACGTR 3 cut(s) 562, 693, 1283
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 1266
BsaJI CCNNGG 1 cut(s) 518
BsaWI WCCGGW 2 cut(s) 542, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 881, 971
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 1234
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 2 cut(s) 182, 520
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 3 cut(s) 131, 1171, 1321
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 3 cut(s) 181, 881, 971
BseMI GCAATG 1 cut(s) 1234
BseNI ACTGG 1 cut(s) 12
BseSI GKGCMC 1 cut(s) 1282
BseXI GCAGC 5 cut(s) 103, 131, 208, 1007, 1126
Bsh1236I CGCG 3 cut(s) 157, 300, 1137
BshFI GGCC 3 cut(s) 20, 820, 1058
BshNI GGYRCC 2 cut(s) 545, 1114
BshTI ACCGGT 1 cut(s) 542
BsiHKAI GWGCWC 2 cut(s) 703, 1282
BsiHKCI CYCGRG 1 cut(s) 387
BsiSI CCGG 2 cut(s) 543, 1112
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 3 cut(s) 181, 881, 971
BsmAI GTCTC 2 cut(s) 106, 655
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 462
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 3 cut(s) 20, 820, 1058
BsoBI CYCGRG 1 cut(s) 387
Bsp119I TTCGAA 1 cut(s) 1099
Bsp1286I GDGCHC 2 cut(s) 703, 1282
Bsp1407I TGTACA 1 cut(s) 766
Bsp143I GATC 4 cut(s) 477, 772, 916, 1223
BspACI CCGC 5 cut(s) 70, 129, 300, 758, 1135
BspANI GGCC 3 cut(s) 20, 820, 1058
BspFNI CGCG 3 cut(s) 157, 300, 1137
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 2 cut(s) 547, 1116
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 1 cut(s) 780
BspT104I TTCGAA 1 cut(s) 1099
BspT107I GGYRCC 2 cut(s) 545, 1114
BspTI CTTAAG 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 1234
BsrFI RCCGGY 1 cut(s) 542
BsrGI TGTACA 1 cut(s) 766
BsrI ACTGG 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 4 cut(s) 477, 772, 916, 1223
BssNI GRCGYC 1 cut(s) 1266
BssSI CACGAG 1 cut(s) 442
Bst2BI CACGAG 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 182, 520
Bst4CI ACNGT 3 cut(s) 247, 506, 1351
Bst6I CTCTTC 1 cut(s) 1197
BstACI GRCGYC 1 cut(s) 1266
BstAFI CTTAAG 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 766
BstBAI YACGTR 3 cut(s) 562, 693, 1283
BstBI TTCGAA 1 cut(s) 1099
BstC8I GCNNGC 2 cut(s) 67, 1056
BstEII GGTNACC 1 cut(s) 1229
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 3 cut(s) 131, 1171, 1321
BstFNI CGCG 3 cut(s) 157, 300, 1137
BstHHI GCGC 2 cut(s) 1139, 1241
BstKTI GATC 4 cut(s) 480, 775, 919, 1226
BstMAI GTCTC 2 cut(s) 106, 655
BstMBI GATC 4 cut(s) 477, 772, 916, 1223
BstMWI GCNNNNNNNGC 4 cut(s) 113, 128, 383, 419
BstNI CCWGG 2 cut(s) 182, 520
BstPI GGTNACC 1 cut(s) 1229
BstSCI CCNGG 2 cut(s) 180, 518
BstSFI CTRYAG 2 cut(s) 117, 1176
BstSLI GKGCMC 1 cut(s) 1282
BstUI CGCG 3 cut(s) 157, 300, 1137
BstV1I GCAGC 5 cut(s) 103, 131, 208, 1007, 1126
BstX2I RGATCY 2 cut(s) 477, 772
BstXI CCANNNNNNTGG 1 cut(s) 1208
BstYI RGATCY 2 cut(s) 477, 772
BsuI GTATCC 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 20, 820, 1058
BtgZI GCGATG 1 cut(s) 281
BtsCI GGATG 3 cut(s) 131, 1171, 1321
BtsIMutI CAGTG 3 cut(s) 5, 252, 511
Cac8I GCNNGC 2 cut(s) 67, 1056
CciI TCATGA 1 cut(s) 703
CfoI GCGC 2 cut(s) 1139, 1241
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 2 cut(s) 818, 1109
CseI GACGC 2 cut(s) 163, 1255
Csp6I GTAC 1 cut(s) 767
CspAI ACCGGT 1 cut(s) 542
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviAII CATG 8 cut(s) 49, 647, 704, 754, 868, 1037, 1208, 1334
CviQI GTAC 1 cut(s) 767
DpnI GATC 4 cut(s) 479, 774, 918, 1225
DpnII GATC 4 cut(s) 477, 772, 916, 1223
EaeI YGGCCR 1 cut(s) 1056
Eam1104I CTCTTC 1 cut(s) 1197
EarI CTCTTC 1 cut(s) 1197
Eco32I GATATC 2 cut(s) 688, 900
Eco47I GGWCC 1 cut(s) 1109
Eco57I CTGAAG 2 cut(s) 438, 1104
Eco72I CACGTG 2 cut(s) 693, 1283
Eco88I CYCGRG 1 cut(s) 387
Eco91I GGTNACC 1 cut(s) 1229
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 1229
EcoRII CCWGG 2 cut(s) 180, 518
EcoRV GATATC 2 cut(s) 688, 900
EcoT22I ATGCAT 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 8 cut(s) 52, 650, 707, 757, 871, 1040, 1211, 1337
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 1 cut(s) 462
FatI CATG 8 cut(s) 48, 646, 703, 753, 867, 1036, 1207, 1333
FauI CCCGC 2 cut(s) 136, 293
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 6 cut(s) 70, 117, 120, 222, 996, 1140
FokI GGATG 3 cut(s) 118, 1158, 1328
Fsp4HI GCNGC 6 cut(s) 70, 117, 120, 222, 996, 1140
FspBI CTAG 6 cut(s) 198, 405, 662, 945, 989, 1308
GlaI GCGC 2 cut(s) 1138, 1240
GluI GCNGC 6 cut(s) 70, 117, 120, 222, 996, 1140
HaeIII GGCC 3 cut(s) 20, 820, 1058
HapII CCGG 2 cut(s) 543, 1112
HgaI GACGC 2 cut(s) 163, 1255
HhaI GCGC 2 cut(s) 1139, 1241
Hin1I GRCGYC 1 cut(s) 1266
Hin1II CATG 8 cut(s) 52, 650, 707, 757, 871, 1040, 1211, 1337
Hin6I GCGC 2 cut(s) 1137, 1239
HinP1I GCGC 2 cut(s) 1137, 1239
HinfI GANTC 6 cut(s) 304, 450, 834, 948, 1288, 1356
HpaII CCGG 2 cut(s) 543, 1112
HphI GGTGA 2 cut(s) 170, 1223
Hpy166II GTNNAC 3 cut(s) 208, 1132, 1280
Hpy188I TCNGA 7 cut(s) 364, 382, 741, 904, 1123, 1223, 1355
Hpy8I GTNNAC 3 cut(s) 208, 1132, 1280
Hpy99I CGWCG 3 cut(s) 93, 157, 1271
HpyAV CCTTC 5 cut(s) 5, 94, 185, 1128, 1259
HpyCH4III ACNGT 3 cut(s) 247, 506, 1351
HpyCH4IV ACGT 4 cut(s) 291, 561, 692, 1282
HpyCH4V TGCA 8 cut(s) 119, 224, 262, 295, 377, 1027, 1274, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 128, 383, 419
HpySE526I ACGT 4 cut(s) 291, 561, 692, 1282
Hsp92I GRCGYC 1 cut(s) 1266
Hsp92II CATG 8 cut(s) 52, 650, 707, 757, 871, 1040, 1211, 1337
HspAI GCGC 2 cut(s) 1137, 1239
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 4 cut(s) 477, 772, 916, 1223
LmnI GCTCC 2 cut(s) 127, 992
Lsp1109I GCAGC 5 cut(s) 103, 131, 208, 1007, 1126
LweI GCATC 5 cut(s) 140, 304, 373, 386, 721
MaeI CTAG 6 cut(s) 198, 405, 662, 945, 989, 1308
MaeII ACGT 4 cut(s) 291, 561, 692, 1282
MaeIII GTNAC 5 cut(s) 146, 241, 278, 851, 1229
MalI GATC 4 cut(s) 479, 774, 918, 1225
MboI GATC 4 cut(s) 477, 772, 916, 1223
MboII GAAGA 7 cut(s) 367, 587, 665, 695, 855, 1136, 1184
MfeI CAATTG 3 cut(s) 489, 969, 981
MflI RGATCY 2 cut(s) 477, 772
MhlI GDGCHC 2 cut(s) 703, 1282
MlsI TGGCCA 1 cut(s) 1058
MluNI TGGCCA 1 cut(s) 1058
MlyI GAGTC 2 cut(s) 444, 1297
MnlI CCTC 8 cut(s) 87, 146, 811, 882, 946, 948, 952, 1306
Mox20I TGGCCA 1 cut(s) 1058
Mph1103I ATGCAT 1 cut(s) 379
MscI TGGCCA 1 cut(s) 1058
MseI TTAA 6 cut(s) 23, 251, 314, 602, 777, 1362
MslI CAYNNNNRTG 2 cut(s) 504, 1041
Msp20I TGGCCA 1 cut(s) 1058
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 2 cut(s) 543, 1112
MspR9I CCNGG 2 cut(s) 182, 520
MunI CAATTG 3 cut(s) 489, 969, 981
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 2 cut(s) 182, 520
MvnI CGCG 3 cut(s) 157, 300, 1137
MwoI GCNNNNNNNGC 4 cut(s) 113, 128, 383, 419
NdeII GATC 4 cut(s) 477, 772, 916, 1223
NlaIII CATG 8 cut(s) 52, 650, 707, 757, 871, 1040, 1211, 1337
NlaIV GGNNCC 2 cut(s) 547, 1116
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 4 cut(s) 146, 241, 851, 1229
NsiI ATGCAT 1 cut(s) 379
NspV TTCGAA 1 cut(s) 1099
PaeR7I CTCGAG 1 cut(s) 387
PagI TCATGA 1 cut(s) 703
PctI GAATGC 1 cut(s) 415
PfeI GAWTC 4 cut(s) 304, 834, 948, 1356
PflFI GACNNNGTC 1 cut(s) 1349
PflMI CCANNNNNTGG 1 cut(s) 971
PinAI ACCGGT 1 cut(s) 542
PkrI GCNGC 6 cut(s) 71, 118, 121, 223, 997, 1141
PleI GAGTC 2 cut(s) 444, 1296
PmaCI CACGTG 2 cut(s) 693, 1283
PmlI CACGTG 2 cut(s) 693, 1283
PpsI GAGTC 2 cut(s) 444, 1296
Ppu21I YACGTR 3 cut(s) 562, 693, 1283
Psp6I CCWGG 2 cut(s) 180, 518
PspCI CACGTG 2 cut(s) 693, 1283
PspEI GGTNACC 1 cut(s) 1229
PspGI CCWGG 2 cut(s) 180, 518
PspN4I GGNNCC 2 cut(s) 547, 1116
PspPI GGNCC 2 cut(s) 818, 1109
PstI CTGCAG 1 cut(s) 121
PsuI RGATCY 2 cut(s) 477, 772
PsyI GACNNNGTC 1 cut(s) 1349
RsaI GTAC 1 cut(s) 768
RsaNI GTAC 1 cut(s) 767
RseI CAYNNNNRTG 2 cut(s) 504, 1041
SaqAI TTAA 6 cut(s) 23, 251, 314, 602, 777, 1362
SatI GCNGC 6 cut(s) 70, 117, 120, 222, 996, 1140
Sau3AI GATC 4 cut(s) 477, 772, 916, 1223
Sau96I GGNCC 2 cut(s) 818, 1109
SchI GAGTC 2 cut(s) 444, 1297
ScrFI CCNGG 2 cut(s) 182, 520
SduI GDGCHC 2 cut(s) 703, 1282
SfaNI GCATC 5 cut(s) 140, 304, 373, 386, 721
SfcI CTRYAG 2 cut(s) 117, 1176
Sfr274I CTCGAG 1 cut(s) 387
SfuI TTCGAA 1 cut(s) 1099
SinI GGWCC 1 cut(s) 1109
SlaI CTCGAG 1 cut(s) 387
SmiMI CAYNNNNRTG 2 cut(s) 504, 1041
SmlI CTYRAG 2 cut(s) 313, 387
SmoI CTYRAG 2 cut(s) 313, 387
SsiI CCGC 5 cut(s) 70, 129, 300, 758, 1135
SspMI CTAG 6 cut(s) 198, 405, 662, 945, 989, 1308
StyD4I CCNGG 2 cut(s) 180, 518
TaaI ACNGT 3 cut(s) 247, 506, 1351
TaiI ACGT 4 cut(s) 294, 564, 695, 1285
TaqI TCGA 6 cut(s) 152, 388, 951, 1099, 1161, 1255
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 4 cut(s) 304, 834, 948, 1356
Tru1I TTAA 6 cut(s) 23, 251, 314, 602, 777, 1362
Tru9I TTAA 6 cut(s) 23, 251, 314, 602, 777, 1362
TscAI CASTG 3 cut(s) 12, 252, 511
TseFI GTSAC 4 cut(s) 146, 241, 851, 1229
TseI GCWGC 5 cut(s) 116, 119, 221, 995, 1139
Tsp45I GTSAC 4 cut(s) 146, 241, 851, 1229
TspDTI ATGAA 7 cut(s) 30, 296, 354, 588, 632, 1053, 1241
TspGWI ACGGA 2 cut(s) 77, 1045
TspRI CASTG 3 cut(s) 12, 252, 511
Tth111I GACNNNGTC 1 cut(s) 1349
Van91I CCANNNNNTGG 1 cut(s) 971
Vha464I CTTAAG 1 cut(s) 313
VneI GTGCAC 1 cut(s) 1278
VpaK11BI GGWCC 1 cut(s) 1109
XagI CCTNNNNNAGG 1 cut(s) 179
XapI RAATTY 2 cut(s) 365, 1101
XbaI TCTAGA 2 cut(s) 197, 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XhoI CTCGAG 1 cut(s) 387
XspI CTAG 6 cut(s) 198, 405, 662, 945, 989, 1308
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.