Rh1DG104500

Cysteine desulfurase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006305
Physical Location & Seq
Reverse (-)
19578054 .. 19586311
8258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG104500.1

Sequence Viewer

Length: 1380 bp
ATGATGACACTGGAAGGAGTGGCCTTAAAATTGACCCCATATTCATTCCCCATGATCACAACCCCTGCCTGCCGCACCCCTCACACCTTCCGACGCTTTCTCGTCTCGGCTTCAACTGCTGCAGCTCCACCCGCATCCCTCTCACTTGGTCACTCGACCCGACCCGATTTCCCTATCCTTCACCAGGAAGTAAATGGCTCTAGACTTGTTTACTTGGACAATGCTGCAACTTCCCAGAAGCCCATCACTGTGTTAAAGGCTTTGCAGGATTACTATCAAGGTTACAATTCAAACGTGCATCGCGGGATTCATTACTTAAGTTCAAAGGCAACAATGGAATATGAATTGGCGAGAAAGAAGATTTCGGAATTTATCAATGCATCAGATGCTCGAGAAATTGTTTTTACTAGGAATGCTACTGAAGCTATCAATCTGGTTGCACACTCGTGGGGACTCCAAAATATAAAATCAAATGATGAGATCTTACTCACAATTGCCGAACATCACAGTGCGATTGTTCCCTGGCAACTTGTAGCTCAAAAGACCGGTGCCATTTTGAAATACGTGGAACTAAATGAAGATGGAGTTCCAGATGTGGAAAAGTTAAAAGATATGCTTTCAAGAAAGACAAAACTTGTAGTTGTTCATCATGTCTCAAATGTGCTAGGTTCTGTTCTTCCTATTGAAGATATCACGTGTTGTGCTCATGATGTTGGGGCAAAAGTTCTTGTAGATGCTTGTCAGAGTGTTCCACACATGGCGGTTGATGTACAGGATCTTAATGCTGACTTTCTTGTTGCTTCCTCTCACAAGATGTGTGGGCCTACGGGAATTGGATTCTTATACGGCAAGAGTGACCTATTTTCTTCCATGCCTCCATTTTTAGGTGGTGGAGAAATGATATCTGATGTATTTCTTGATCATTCCACTTATGCCGAACCTCCATCTAGATTCGAGGCTGGAACACCAGCAATTGGGGAAGCAATTGGGCTAGGAGCAGCTATTGATTATTTGTCTGGAATTGGTATGCAACGGATACATGAGTATGAGATAGAGCTGGCCAAATATCTATATAATAGTCTTTGTTCAATACCCAACATTCGAATTTATGGTCCGGTGCCTTCAGAAGATGTCCACCGCGCTGCTCTTTGTTCTTTCAATGTTGAGAATATACACCCTACAGATTTGGCAACTCTTCTTGACCAACAGCATGGAGTGGCTATCCGATCAGGTCACCATTGCACCCAACCCCTTCATCAACATTTAGGCGTCGGTGCAAGTGCACGTGCGAGTCTTTACTTCTACAACACTAGAGAGGATGTTGATGACTTTATCCATGCCCTAAATGATACAGTCAGATTCTTTAATTCTTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00450 ko01100 map00450 map01100
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

459

Amino Acids

50.44

Weight (kDa)

6.0

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 70 - 444 1.4e-146 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 123 - 251 6.3e-07 DegT/DnrJ/EryC1/StrS aminotransferase family
Cys_Met_Meta_PP PF01053 156 - 271 2.7e-06 Cys/Met metabolism PLP-dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 548, 1117
AccB7I CCANNNNNTGG 1 cut(s) 974
AccII CGCG 2 cut(s) 303, 1140
AciI CCGC 5 cut(s) 73, 132, 303, 761, 1138
AclWI GGATC 1 cut(s) 783
AcoI YGGCCR 1 cut(s) 1059
AcsI RAATTY 2 cut(s) 368, 1104
AcuI CTGAAG 2 cut(s) 441, 1107
AcvI CACGTG 2 cut(s) 696, 1286
AcyI GRCGYC 1 cut(s) 1269
AfaI GTAC 1 cut(s) 771
AfiI CCNNNNNNNGG 3 cut(s) 184, 884, 974
AflII CTTAAG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 695
AgeI ACCGGT 1 cut(s) 545
AgsI TTSAA 9 cut(s) 114, 291, 324, 559, 621, 686, 1089, 1159, 1375
AjnI CCWGG 2 cut(s) 183, 521
AleI CACNNNNGTG 1 cut(s) 445
AluBI AGCT 5 cut(s) 125, 425, 536, 1001, 1057
AluI AGCT 5 cut(s) 125, 425, 536, 1001, 1057
Alw21I GWGCWC 2 cut(s) 706, 1285
Alw26I GTCTC 2 cut(s) 109, 658
Alw44I GTGCAC 1 cut(s) 1281
AlwI GGATC 1 cut(s) 783
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 3 cut(s) 21, 821, 1059
ApaLI GTGCAC 1 cut(s) 1281
ApeKI GCWGC 5 cut(s) 119, 122, 224, 998, 1142
ApoI RAATTY 2 cut(s) 368, 1104
ArsI GACNNNNNNTTYG 2 cut(s) 1096, 1128
AsiGI ACCGGT 1 cut(s) 545
AspLEI GCGC 1 cut(s) 1142
AspS9I GGNCC 2 cut(s) 821, 1112
AsuHPI GGTGA 2 cut(s) 173, 1226
AsuII TTCGAA 1 cut(s) 1102
AvaI CYCGRG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 1112
BaeGI GKGCMC 1 cut(s) 1285
BaeI ACNNNNGTAYC 2 cut(s) 1028, 1061
BalI TGGCCA 1 cut(s) 1061
BanI GGYRCC 2 cut(s) 548, 1117
BauI CACGAG 1 cut(s) 445
BbrPI CACGTG 2 cut(s) 696, 1286
Bbv12I GWGCWC 2 cut(s) 706, 1285
BbvI GCAGC 5 cut(s) 106, 134, 211, 1010, 1129
BccI CCATC 3 cut(s) 251, 575, 952
BceAI ACGGC 1 cut(s) 862
BciT130I CCWGG 2 cut(s) 185, 523
BciVI GTATCC 1 cut(s) 1029
BclI TGATCA 2 cut(s) 54, 919
BcoDI GTCTC 2 cut(s) 109, 658
BfaI CTAG 6 cut(s) 201, 408, 665, 948, 992, 1311
BfmI CTRYAG 2 cut(s) 120, 1179
BfrI CTTAAG 1 cut(s) 316
BfuI GTATCC 1 cut(s) 1029
BglII AGATCT 1 cut(s) 480
BisI GCNGC 6 cut(s) 73, 120, 123, 225, 999, 1143
BlsI GCNGC 6 cut(s) 74, 121, 124, 226, 1000, 1144
Bme1390I CCNGG 2 cut(s) 185, 523
Bme18I GGWCC 1 cut(s) 1112
BmeT110I CYCGRG 1 cut(s) 390
BmgT120I GGNCC 2 cut(s) 821, 1112
BmiI GGNNCC 2 cut(s) 550, 1119
BmrFI CCNGG 2 cut(s) 185, 523
BmsI GCATC 5 cut(s) 143, 307, 376, 389, 724
Bpu14I TTCGAA 1 cut(s) 1102
BsaAI YACGTR 3 cut(s) 565, 696, 1286
BsaBI GATNNNNATC 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 1269
BsaJI CCNNGG 1 cut(s) 521
BsaWI WCCGGW 2 cut(s) 545, 1114
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 884, 974
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 1237
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 2 cut(s) 185, 523
BseDI CCNNGG 1 cut(s) 521
BseGI GGATG 2 cut(s) 134, 1324
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 184, 884, 974
BseMI GCAATG 1 cut(s) 1237
BseNI ACTGG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 1285
BseXI GCAGC 5 cut(s) 106, 134, 211, 1010, 1129
Bsh1236I CGCG 2 cut(s) 303, 1140
BshFI GGCC 3 cut(s) 23, 823, 1061
BshNI GGYRCC 2 cut(s) 548, 1117
BshTI ACCGGT 1 cut(s) 545
BsiHKAI GWGCWC 2 cut(s) 706, 1285
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 2 cut(s) 546, 1115
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 3 cut(s) 184, 884, 974
BsmAI GTCTC 2 cut(s) 109, 658
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 3 cut(s) 23, 823, 1061
BsoBI CYCGRG 1 cut(s) 390
Bsp119I TTCGAA 1 cut(s) 1102
Bsp1286I GDGCHC 2 cut(s) 706, 1285
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 5 cut(s) 54, 480, 775, 919, 1226
BspACI CCGC 5 cut(s) 73, 132, 303, 761, 1138
BspANI GGCC 3 cut(s) 23, 823, 1061
BspFNI CGCG 2 cut(s) 303, 1140
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 2 cut(s) 550, 1119
BspMAI CTGCAG 1 cut(s) 124
BspPI GGATC 1 cut(s) 783
BspT104I TTCGAA 1 cut(s) 1102
BspT107I GGYRCC 2 cut(s) 548, 1117
BspTI CTTAAG 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 1237
BsrFI RCCGGY 1 cut(s) 545
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 1 cut(s) 521
BssMI GATC 5 cut(s) 54, 480, 775, 919, 1226
BssNI GRCGYC 1 cut(s) 1269
BssSI CACGAG 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 445
Bst2UI CCWGG 2 cut(s) 185, 523
Bst4CI ACNGT 3 cut(s) 250, 509, 1354
Bst6I CTCTTC 1 cut(s) 1200
BstACI GRCGYC 1 cut(s) 1269
BstAFI CTTAAG 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 769
BstBAI YACGTR 3 cut(s) 565, 696, 1286
BstBI TTCGAA 1 cut(s) 1102
BstC8I GCNNGC 2 cut(s) 70, 1059
BstEII GGTNACC 1 cut(s) 1232
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 134, 1324
BstFNI CGCG 2 cut(s) 303, 1140
BstHHI GCGC 1 cut(s) 1142
BstKTI GATC 5 cut(s) 57, 483, 778, 922, 1229
BstMAI GTCTC 2 cut(s) 109, 658
BstMBI GATC 5 cut(s) 54, 480, 775, 919, 1226
BstMWI GCNNNNNNNGC 4 cut(s) 116, 131, 386, 422
BstNI CCWGG 2 cut(s) 185, 523
BstPI GGTNACC 1 cut(s) 1232
BstSCI CCNGG 2 cut(s) 183, 521
BstSFI CTRYAG 2 cut(s) 120, 1179
BstSLI GKGCMC 1 cut(s) 1285
BstUI CGCG 2 cut(s) 303, 1140
BstV1I GCAGC 5 cut(s) 106, 134, 211, 1010, 1129
BstX2I RGATCY 2 cut(s) 480, 775
BstXI CCANNNNNNTGG 1 cut(s) 1211
BstYI RGATCY 2 cut(s) 480, 775
BsuI GTATCC 1 cut(s) 1029
BsuRI GGCC 3 cut(s) 23, 823, 1061
BtgZI GCGATG 1 cut(s) 284
BtsCI GGATG 2 cut(s) 134, 1324
BtsIMutI CAGTG 3 cut(s) 8, 246, 514
Cac8I GCNNGC 2 cut(s) 70, 1059
CciI TCATGA 1 cut(s) 706
CfoI GCGC 1 cut(s) 1142
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 2 cut(s) 821, 1112
CseI GACGC 2 cut(s) 102, 1258
Csp6I GTAC 1 cut(s) 770
CspAI ACCGGT 1 cut(s) 545
CspCI CAANNNNNGTGG 2 cut(s) 799, 834
CviAII CATG 8 cut(s) 52, 650, 707, 757, 871, 1040, 1211, 1337
CviQI GTAC 1 cut(s) 770
DpnI GATC 5 cut(s) 56, 482, 777, 921, 1228
DpnII GATC 5 cut(s) 54, 480, 775, 919, 1226
EaeI YGGCCR 1 cut(s) 1059
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
Eco32I GATATC 2 cut(s) 691, 903
Eco47I GGWCC 1 cut(s) 1112
Eco57I CTGAAG 2 cut(s) 441, 1107
Eco72I CACGTG 2 cut(s) 696, 1286
Eco88I CYCGRG 1 cut(s) 390
Eco91I GGTNACC 1 cut(s) 1232
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 183, 521
EcoRV GATATC 2 cut(s) 691, 903
EcoT22I ATGCAT 1 cut(s) 382
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 8 cut(s) 55, 653, 710, 760, 874, 1043, 1214, 1340
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 1 cut(s) 465
FatI CATG 8 cut(s) 51, 649, 706, 756, 870, 1039, 1210, 1336
FauI CCCGC 2 cut(s) 139, 296
FbaI TGATCA 2 cut(s) 54, 919
Fnu4HI GCNGC 6 cut(s) 73, 120, 123, 225, 999, 1143
FokI GGATG 2 cut(s) 121, 1331
Fsp4HI GCNGC 6 cut(s) 73, 120, 123, 225, 999, 1143
FspBI CTAG 6 cut(s) 201, 408, 665, 948, 992, 1311
GlaI GCGC 1 cut(s) 1141
GluI GCNGC 6 cut(s) 73, 120, 123, 225, 999, 1143
HaeIII GGCC 3 cut(s) 23, 823, 1061
HapII CCGG 2 cut(s) 546, 1115
HgaI GACGC 2 cut(s) 102, 1258
HhaI GCGC 1 cut(s) 1142
Hin1I GRCGYC 1 cut(s) 1269
Hin1II CATG 8 cut(s) 55, 653, 710, 760, 874, 1043, 1214, 1340
Hin6I GCGC 1 cut(s) 1140
HinP1I GCGC 1 cut(s) 1140
HinfI GANTC 6 cut(s) 307, 453, 837, 951, 1291, 1359
HpaII CCGG 2 cut(s) 546, 1115
HphI GGTGA 2 cut(s) 173, 1226
Hpy166II GTNNAC 3 cut(s) 211, 1135, 1283
Hpy188I TCNGA 8 cut(s) 92, 367, 385, 744, 907, 1126, 1226, 1358
Hpy188III TCNNGA 9 cut(s) 201, 392, 590, 621, 707, 917, 948, 1017, 1199
Hpy8I GTNNAC 3 cut(s) 211, 1135, 1283
Hpy99I CGWCG 2 cut(s) 96, 1274
HpyAV CCTTC 5 cut(s) 8, 97, 188, 1131, 1262
HpyCH4III ACNGT 3 cut(s) 250, 509, 1354
HpyCH4IV ACGT 4 cut(s) 294, 564, 695, 1285
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 131, 386, 422
HpySE526I ACGT 4 cut(s) 294, 564, 695, 1285
Hsp92I GRCGYC 1 cut(s) 1269
Hsp92II CATG 8 cut(s) 55, 653, 710, 760, 874, 1043, 1214, 1340
HspAI GCGC 1 cut(s) 1140
Ksp22I TGATCA 2 cut(s) 54, 919
Kzo9I GATC 5 cut(s) 54, 480, 775, 919, 1226
LmnI GCTCC 2 cut(s) 130, 995
Lsp1109I GCAGC 5 cut(s) 106, 134, 211, 1010, 1129
LweI GCATC 5 cut(s) 143, 307, 376, 389, 724
MaeI CTAG 6 cut(s) 201, 408, 665, 948, 992, 1311
MaeII ACGT 4 cut(s) 294, 564, 695, 1285
MaeIII GTNAC 4 cut(s) 149, 281, 854, 1232
MalI GATC 5 cut(s) 56, 482, 777, 921, 1228
MboI GATC 5 cut(s) 54, 480, 775, 919, 1226
MboII GAAGA 7 cut(s) 370, 590, 668, 698, 858, 1139, 1187
MfeI CAATTG 3 cut(s) 492, 972, 984
MflI RGATCY 2 cut(s) 480, 775
MhlI GDGCHC 2 cut(s) 706, 1285
MlsI TGGCCA 1 cut(s) 1061
MluNI TGGCCA 1 cut(s) 1061
MlyI GAGTC 2 cut(s) 447, 1300
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 7 cut(s) 90, 149, 814, 885, 949, 951, 1309
Mox20I TGGCCA 1 cut(s) 1061
Mph1103I ATGCAT 1 cut(s) 382
MscI TGGCCA 1 cut(s) 1061
MseI TTAA 6 cut(s) 26, 254, 317, 605, 780, 1365
MslI CAYNNNNRTG 4 cut(s) 248, 445, 507, 1044
Msp20I TGGCCA 1 cut(s) 1061
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 2 cut(s) 546, 1115
MspR9I CCNGG 2 cut(s) 185, 523
MunI CAATTG 3 cut(s) 492, 972, 984
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 2 cut(s) 185, 523
MvnI CGCG 2 cut(s) 303, 1140
MwoI GCNNNNNNNGC 4 cut(s) 116, 131, 386, 422
NdeII GATC 5 cut(s) 54, 480, 775, 919, 1226
NlaIII CATG 8 cut(s) 55, 653, 710, 760, 874, 1043, 1214, 1340
NlaIV GGNNCC 2 cut(s) 550, 1119
NmeAIII GCCGAG 1 cut(s) 86
NmuCI GTSAC 3 cut(s) 149, 854, 1232
NsiI ATGCAT 1 cut(s) 382
NspV TTCGAA 1 cut(s) 1102
OliI CACNNNNGTG 1 cut(s) 445
PaeR7I CTCGAG 1 cut(s) 390
PagI TCATGA 1 cut(s) 706
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 4 cut(s) 307, 837, 951, 1359
PflMI CCANNNNNTGG 1 cut(s) 974
PinAI ACCGGT 1 cut(s) 545
PkrI GCNGC 6 cut(s) 74, 121, 124, 226, 1000, 1144
PleI GAGTC 2 cut(s) 447, 1299
PmaCI CACGTG 2 cut(s) 696, 1286
PmlI CACGTG 2 cut(s) 696, 1286
PpsI GAGTC 2 cut(s) 447, 1299
Ppu21I YACGTR 3 cut(s) 565, 696, 1286
Psp6I CCWGG 2 cut(s) 183, 521
PspCI CACGTG 2 cut(s) 696, 1286
PspEI GGTNACC 1 cut(s) 1232
PspGI CCWGG 2 cut(s) 183, 521
PspN4I GGNNCC 2 cut(s) 550, 1119
PspPI GGNCC 2 cut(s) 821, 1112
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 2 cut(s) 480, 775
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
RseI CAYNNNNRTG 4 cut(s) 248, 445, 507, 1044
SaqAI TTAA 6 cut(s) 26, 254, 317, 605, 780, 1365
SatI GCNGC 6 cut(s) 73, 120, 123, 225, 999, 1143
Sau3AI GATC 5 cut(s) 54, 480, 775, 919, 1226
Sau96I GGNCC 2 cut(s) 821, 1112
SchI GAGTC 2 cut(s) 447, 1300
ScrFI CCNGG 2 cut(s) 185, 523
SduI GDGCHC 2 cut(s) 706, 1285
SfaNI GCATC 5 cut(s) 143, 307, 376, 389, 724
SfcI CTRYAG 2 cut(s) 120, 1179
Sfr274I CTCGAG 1 cut(s) 390
SfuI TTCGAA 1 cut(s) 1102
SinI GGWCC 1 cut(s) 1112
SlaI CTCGAG 1 cut(s) 390
SmiMI CAYNNNNRTG 4 cut(s) 248, 445, 507, 1044
SmlI CTYRAG 2 cut(s) 316, 390
SmoI CTYRAG 2 cut(s) 316, 390
SsiI CCGC 5 cut(s) 73, 132, 303, 761, 1138
SspMI CTAG 6 cut(s) 201, 408, 665, 948, 992, 1311
StyD4I CCNGG 2 cut(s) 183, 521
TaaI ACNGT 3 cut(s) 250, 509, 1354
TaiI ACGT 4 cut(s) 297, 567, 698, 1288
TaqI TCGA 4 cut(s) 155, 391, 954, 1102
TatI WGTACW 1 cut(s) 769
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 4 cut(s) 307, 837, 951, 1359
Tru1I TTAA 6 cut(s) 26, 254, 317, 605, 780, 1365
Tru9I TTAA 6 cut(s) 26, 254, 317, 605, 780, 1365
TscAI CASTG 3 cut(s) 15, 253, 514
TseFI GTSAC 3 cut(s) 149, 854, 1232
TseI GCWGC 5 cut(s) 119, 122, 224, 998, 1142
Tsp45I GTSAC 3 cut(s) 149, 854, 1232
TspDTI ATGAA 6 cut(s) 33, 299, 357, 591, 635, 1244
TspGWI ACGGA 1 cut(s) 1048
TspRI CASTG 3 cut(s) 15, 253, 514
Van91I CCANNNNNTGG 1 cut(s) 974
Vha464I CTTAAG 1 cut(s) 316
VneI GTGCAC 1 cut(s) 1281
VpaK11BI GGWCC 1 cut(s) 1112
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 2 cut(s) 368, 1104
XbaI TCTAGA 2 cut(s) 200, 947
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XhoI CTCGAG 1 cut(s) 390
XspI CTAG 6 cut(s) 201, 408, 665, 948, 992, 1311
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.