Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0009007

Physical Location & Seq

Genomic Coordinates Forward (+)

50561839 .. 50575076

Gene Structure 13238 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00321250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3573 bp

ATGCCTTGCTTTCCCCAACCCGGTTCCGTCACGGTTTGCGAAATCAATCGCGACCTAATCACTTCTGAAAACCTCTCGGATGATGGAGCCAAAGAAACTTACGGAAAGATTCTGGGAATCACATTCAGCCCGGTTCCATTTCAATCCGAAGTTGGGCCTCCACCGAGTCCGGACCGCGAAACAGCTCCCACAAAGGGACTTCTACAAAGCCTATTCCATCCTAACCATGTGAACTTGTTACCGGAAGTTGATCTTCAGGGATTGAGCTGGCACCCACATAAACATGTTATAGCATTTATATCCGGCTCAAATCAAGTTCTAGTTCGAGATTATGAGGATTCAGAAGGGAAGGAGCCGTGCATTTTGGCGAATGAAGCGCAGAAAGACGTCAAGGTACTTGAATGGAGGCCAAATGGTGGGAGGACACTTTCTGTGGCATGTAGGCAAGTAAAAGATCGTGGGATCTGCATTTGGGCCGCTTCTTTCCCTGGCGGTGTTGCATCTGTGAGATCTGGTGCTTCTTCCTTCTTGGGAACTCTCTCCAGAGGGATTGGGACACCTATTCGACGCGGACTAGCTGGCATATCAATACTGAAGTGGTCACCAACTGGCGATTACTTTTTCTCCGCAAAATTTGATGGAACGTTTTATCTTTGGGAGACAAATACATGGGCATCTGAATCATGGTCGTCAACTAGTGGCTTTGTAAAGGTATGCTGTATGTTTCTTGTGAGGATTGCTCAAGTTTCTTGGGGAGCATCATGGGATCCTGATGGGCGTATGATACTTCTTGCCTTCTCTAAATCTTCAACTTTGGGGTCCATTCACTTTGCTTCAAAGCCACCATCACTGGTGCTTAATTCAAGTAGTCAGGATCTTAACTTTAGCAATAGGTCTCCTAGCTTAATGACACTTCCCGCAAGCTGGGAAGCAGACATCAAGGACTCAGGCTTGGTGCAAATAGAATTAAAGGCAGTCTTTGGAAAAGCTTTAGTACAGATATGCGCACCTATTACCTGTCGAACTACCAGAGATTACGGCCTTAACAAACAGGGCATTGAGAAGATAGCTTGGGATGCTTCGGGAGAGCGATTGGCTGTATCTTTTAAAGATTGCAATGAGTTGTATAGAGGTCTGATTGCCATCTATGATGTTAGAAGGACTCCTCTTATCTCTGCATCATTAATTGGATTTATCAGAGGACCTGGGGAGTATCCAAAGCCTATAGCATTTTCATTCCATGACAAGTTTAAGCAAGGACCATTGCTTTCTGTGGCGAAATGTGCCCCATACCAGTTGATTTTAGATATACCAAAACCCTACTCCACATCCAATGGAGAGGAAGCTACGAGAGATAGCAAAGTGACTGTGGCACCCAACCCGAAATGGGGTGCACACCAAACACTAAAATTCCCACCAAATAGACATGTGTGGGATAGGTTGTCGAGGCAACATCACTACATCAAACTCGGTATACACACCACCGTAGCAGTAGCCTTTGCTGCCTCAACAACCATTTTAGTGTGGCTCGGTGGTGGCGCAACGACACCCTGCCTTAGCTGGCCATCAGCTTCTACTCAGTGCGAAATGGTGTTGAAATCACTGTGGCATTCCCACCTTAAATGGGCATTCCAGGTTTCCTACAGAGTTTCGCTTATTACTAAGTTCACAAAGGGGAGAATGCTCCTTTTGGCAAGACTCAAGAGTGGAACCAATCAATTTCAAACCCCTTTAAAGCTAAAGTTTTCTCTTGTGTCCGCTGCTAAAGTATCCAGCCAATGTGTATCGTCTATAGAAACAGATGTCGACATTGATAGGATTACTAGAATTATTAATAACCATCCATATCCTGATCAACCACTTCAACCGGCTCTTCTCCAGAATATCCCACCACCTCTTCTTTCTACGAGCTTTGTGGAGAATGTTCTTGGTCACCTTTTCGGTGCCCATTCCAATGGTCTTAAAGCTCTTGAGTTCTTCAATTATTCCCTCCATCATTCCCAGATGTGTCCAAGTTCAGATGCTTTTGAAAAGACGCTGCATATCCTTACCAGGATGCGCTATTTTGATAAAGCGTGGGAGTTGATGGAGAAAATAAGTAGTATGCATTCTTCGTTGCTTACCCTGAAGTCCATGAGCATCCTGCTGTCAAAAATTGCAAAGTTTCAATCCTATGAAGATGTCCTTTTAGCATTTGAAAAGATGGAGACTGATATTTTTGTTGGGAGGAAGTTCGGTACTGATGAGTTTAATGTACTCCTTAGAGCATTTTGCACACAAAGACAAATGAAGGAGGCACGCTCAGTTTTCATAAAGATGCATTCTCGGTTTTCACCCGACACCAAGACCATGAATATCTTGCTCTTGGGGTTCAAGGAAGCTGGAGATATTACTGCAGTAGAACTCTTTTACCATGAGCTTGCTAGAAGAGGCTTTAAGCCAAACAGTATAACTTATAATATTAGGATTGATGCTTACTGTAAAAGAGGTTGCTTTGCTGACGGTTTGAGGCTTTTTGAAGAAATGGAAAGGGCCAAATTGTCGCCTGCATTACAGACTATCACTACACTGATTCATGGAGCTGGGGTTGTTCGGAATCCAATTAAGGCACAACAGTTATTTGATGAGATTCATTTGAGAAATTTGCTTCCTGATACTGGGGCTTATAATGCTTTAATGAGTTCACTTATTAGATCCAATGATGTGCAGTCGGCAGTTGCCTTGATGGATGAGATGGAAGAGAAGCATATTAAGCATGATCATATGACTTATCACACCATGTTTTCTGGCCTGATGAGAACATCGGGTATTGAGGGGGCTTCTGATCTGTATTACAAAATGGTTGATAGAATTTTTGTTCCCAAAACACGAACAGTGGTGATGTTAATTAAATTTTTCTGTGCAAATCATCAGCTTGATTTGGGTTTGCACTTGTGGGGATACCTAGTCGAGAAAGGATATTGCCCTCATGGTCATGCATTGGATCTTTTGTTAACAGCATTATGCTCCCGCAGTAGGGCAATAGAAGCTTTTGAGTGCTCCTTACAAATGTTGGAGAGAGGGAGGGATATGAGTGAAGCATCATTTCGGACATTAGAGACTTTTCTGCTTCAAGCTGGTGAGACAGACAAGCTGAACGGATTGAAATTTCTTCCCAAAAAGCCGGCAGCTGTTCATGATGGAGAGCATGTTTTTGGTGTCGCTCACATCTTTGCCTCCGTCAATGATACCATCACTCATGTGACTGATTTGTCAGGGAGGGAAACTCTTGTCCACATCACTGGTATGGTGTTCTACTCATCTCTTTGTTTATACTGGAATAATATTGGGTACAATTGGTTTATACTGGTGTTGAATTACCTAAGTTGTGCTCTGCTGGACAAGTCAGTGGACTCTAGTGAGCCGGAGGAGTCGGTGTTTGAGTTGTCTAAGAGCGAAGAGGAGAAGGCAGGGGTGGAGTGGAAGCCGCTGGAGGTGAAGCCCAGGAGAACATCAGGGGTGAAGATTGTGACCGGTTTGGCTTCAGTCATGGAGGATGAAAATAGGATTTTGATCCTTCTTTTTCACTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

1190

Amino Acids

133.07

Weight (kDa)

8.46

Isoelectric Point (pI)

39.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Aladin	PF25460	85 - 159	1.2e-07	Aladin seven-bladed propeller
Beta-prop_Aladin	PF25460	186 - 426	2e-18	Aladin seven-bladed propeller
PPR_2	PF13041	750 - 791	2.5e-06	PPR repeat family
PPR_2	PF13041	779 - 828	5.3e-10	PPR repeat family
PPR_1	PF12854	810 - 843	1.2e-07	PPR repeat
PPR_2	PF13041	814 - 860	2.1e-10	PPR repeat family
PPR	PF01535	817 - 845	4.3e-06	PPR repeat
PPR_long	PF17177	830 - 951	5e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	884 - 932	5.1e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61360
fragaria_vesca	FvH4_7g04300 FvH4_7g04300 FvH4_7g04300 FvH4_7g04300
malus_domestica	MD03G1030500.v1.1 MD11G1035500.v1.1
prunus_persica	Prupe.2G028200_v2.0.a1 Prupe.2G028200_v2.0.a1 Prupe.2G028200_v2.0.a1 Prupe.2G028200_v2.0.a1
pyrus_communis	pycom11g02950
rosa_chinensis	RchiOBHm_Chr1g0329261
rosa_laevigata	RLG00000029906
rosa_multiflora	Rmu_sc0035610.1_g000001
rosa_roxburghii	Rroxscaffold_4G00321250
rosa_rugosa	Rorug01G0077600
rosa_samantha	Rh1AG096500 Rh1BG077200 Rh1CG093200 Rh1DG099900
rosa_wichuraiana	Rw1G007470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2457, 2667
AasI	GACNNNNNNGTC	1 cut(s)	3250
AatII	GACGTC	1 cut(s)	390
Acc16I	TGCGCA	1 cut(s)	1006
AccB1I	GGYRCC	3 cut(s)	270, 1372, 1943
AccB7I	CCANNNNNTGG	1 cut(s)	416
AccI	GTMKAC	2 cut(s)	1474, 1806
AccII	CGCG	3 cut(s)	51, 177, 570
AccIII	TCCGGA	1 cut(s)	169
AciI	CCGC	9 cut(s)	175, 477, 492, 570, 627, 918, 1758, 3010, 3467
AclI	AACGTT	1 cut(s)	644
AclWI	GGATC	7 cut(s)	470, 761, 774, 882, 2688, 2991, 3547
AcoI	YGGCCR	1 cut(s)	1562
AcsI	RAATTY	6 cut(s)	632, 1409, 2641, 2850, 2891, 3146
AcuI	CTGAAG	4 cut(s)	239, 614, 2147, 3507
AcyI	GRCGYC	1 cut(s)	387
AfaI	GTAC	5 cut(s)	396, 996, 2239, 2256, 3332
AflIII	ACRYGT	2 cut(s)	283, 1426
AgeI	ACCGGT	1 cut(s)	3512
AhlI	ACTAGT	1 cut(s)	695
AjnI	CCWGG	5 cut(s)	487, 1204, 1632, 2051, 3482
AleI	CACNNNNGTG	1 cut(s)	3239
Alw21I	GWGCWC	3 cut(s)	1396, 3041, 3373
Alw26I	GTCTC	5 cut(s)	653, 900, 2201, 3092, 3116
Alw44I	GTGCAC	1 cut(s)	1392
AlwI	GGATC	7 cut(s)	470, 761, 774, 882, 2688, 2991, 3547
Aor13HI	TCCGGA	1 cut(s)	169
AoxI	GGCC	7 cut(s)	155, 407, 474, 1039, 1562, 2532, 2788
ApaLI	GTGCAC	1 cut(s)	1392
ApeKI	GCWGC	4 cut(s)	1502, 1760, 2038, 3167
ApoI	RAATTY	6 cut(s)	632, 1409, 2641, 2850, 2891, 3146
AseI	ATTAAT	2 cut(s)	1184, 1833
AsiGI	ACCGGT	1 cut(s)	3512
AspLEI	GCGC	4 cut(s)	379, 1007, 1541, 2061
AspS9I	GGNCC	7 cut(s)	155, 172, 474, 819, 1202, 1259, 2532
AsuC2I	CCSGG	2 cut(s)	21, 131
AsuHPI	GGTGA	7 cut(s)	594, 1925, 2325, 2890, 3131, 3487, 3511
AvaII	GGWCC	4 cut(s)	172, 819, 1202, 1259
BaeGI	GKGCMC	3 cut(s)	1288, 1396, 1948
BalI	TGGCCA	1 cut(s)	1564
BamHI	GGATCC	1 cut(s)	766
BanI	GGYRCC	3 cut(s)	270, 1372, 1943
BarI	GAAGNNNNNNTAC	2 cut(s)	2095, 2127
Bbv12I	GWGCWC	3 cut(s)	1396, 3041, 3373
BbvI	GCAGC	4 cut(s)	1489, 1747, 2025, 3179
BceAI	ACGGC	2 cut(s)	340, 1054
BciT130I	CCWGG	5 cut(s)	489, 1206, 1634, 2053, 3484
BciVI	GTATCC	3 cut(s)	1224, 1780, 2933
BclI	TGATCA	2 cut(s)	1852, 2758
BcnI	CCSGG	2 cut(s)	21, 131
BcoDI	GTCTC	5 cut(s)	653, 900, 2201, 3092, 3116
BcuI	ACTAGT	1 cut(s)	695
BfaI	CTAG	8 cut(s)	320, 575, 696, 900, 1824, 2424, 2945, 3396
BfmI	CTRYAG	4 cut(s)	1224, 1642, 1791, 2394
BfuI	GTATCC	3 cut(s)	1224, 1780, 2933
BglII	AGATCT	1 cut(s)	509
BisI	GCNGC	6 cut(s)	477, 1503, 1761, 2039, 3168, 3467
BlsI	GCNGC	6 cut(s)	478, 1504, 1762, 2040, 3169, 3468
Bme1390I	CCNGG	7 cut(s)	21, 131, 489, 1206, 1634, 2053, 3484
Bme18I	GGWCC	4 cut(s)	172, 819, 1202, 1259
BmgT120I	GGNCC	7 cut(s)	155, 172, 474, 819, 1202, 1259, 2532
BmrFI	CCNGG	7 cut(s)	21, 131, 489, 1206, 1634, 2053, 3484
BmrI	ACTGGG	1 cut(s)	2667
BmuI	ACTGGG	1 cut(s)	2667
BplI	GAGNNNNNCTC	6 cut(s)	724, 756, 2285, 2317, 3250, 3282
BpmI	CTGGAG	4 cut(s)	526, 1862, 2403, 3491
Bpu10I	CCTNAGC	1 cut(s)	1556
BpuEI	CTTGAG	3 cut(s)	726, 1685, 1991
BpuMI	CCSGG	2 cut(s)	21, 131
BsaBI	GATNNNNATC	2 cut(s)	1142, 3551
BsaHI	GRCGYC	1 cut(s)	387
BsaI	GGTCTC	1 cut(s)	900
BsaJI	CCNNGG	3 cut(s)	487, 1205, 3482
BsaWI	WCCGGW	3 cut(s)	169, 241, 3512
BsaXI	ACNNNNNCTCC	8 cut(s)	608, 638, 2571, 2601, 3174, 3204, 3401, 3431
Bse118I	RCCGGY	3 cut(s)	1867, 3163, 3512
Bse1I	ACTGG	7 cut(s)	613, 855, 1294, 2662, 3286, 3320, 3351
Bse3DI	GCAATG	2 cut(s)	1123, 1262
Bse8I	GATNNNNATC	2 cut(s)	1142, 3551
BseAI	TCCGGA	1 cut(s)	169
BseBI	CCWGG	5 cut(s)	489, 1206, 1634, 2053, 3484
BseDI	CCNNGG	3 cut(s)	487, 1205, 3482
BseGI	GGATG	9 cut(s)	85, 217, 1081, 1328, 1840, 2061, 2139, 2734, 3541
BseJI	GATNNNNATC	2 cut(s)	1142, 3551
BseMI	GCAATG	2 cut(s)	1123, 1262
BseMII	CTCAG	3 cut(s)	960, 1592, 2316
BseNI	ACTGG	7 cut(s)	613, 855, 1294, 2662, 3286, 3320, 3351
BseRI	GAGGAG	3 cut(s)	1155, 3422, 3455
BseSI	GKGCMC	3 cut(s)	1288, 1396, 1948
BseXI	GCAGC	4 cut(s)	1489, 1747, 2025, 3179
BseYI	CCCAGC	2 cut(s)	924, 2582
BsgI	GTGCAG	1 cut(s)	2726
Bsh1236I	CGCG	3 cut(s)	51, 177, 570
BshFI	GGCC	7 cut(s)	157, 409, 476, 1041, 1564, 2534, 2790
BshNI	GGYRCC	3 cut(s)	270, 1372, 1943
BshTI	ACCGGT	1 cut(s)	3512
BsiHKAI	GWGCWC	3 cut(s)	1396, 3041, 3373
BsiSI	CCGG	9 cut(s)	21, 131, 170, 242, 303, 1868, 3164, 3404, 3513
BslFI	GGGAC	2 cut(s)	210, 568
BsmAI	GTCTC	5 cut(s)	653, 900, 2201, 3092, 3116
BsmFI	GGGAC	2 cut(s)	210, 568
BsmI	GAATGC	5 cut(s)	1609, 1628, 1686, 2107, 2320
BsnI	GGCC	7 cut(s)	157, 409, 476, 1041, 1564, 2534, 2790
Bso31I	GGTCTC	1 cut(s)	900
Bsp1286I	GDGCHC	5 cut(s)	1288, 1396, 1948, 3041, 3373
Bsp13I	TCCGGA	1 cut(s)	169
Bsp68I	TCGCGA	1 cut(s)	51
BspACI	CCGC	9 cut(s)	175, 477, 492, 570, 627, 918, 1758, 3010, 3467
BspANI	GGCC	7 cut(s)	157, 409, 476, 1041, 1564, 2534, 2790
BspCNI	CTCAG	3 cut(s)	959, 1591, 2315
BspEI	TCCGGA	1 cut(s)	169
BspFNI	CGCG	3 cut(s)	51, 177, 570
BspHI	TCATGA	1 cut(s)	3175
BspMAI	CTGCAG	1 cut(s)	2398
BspPI	GGATC	7 cut(s)	470, 761, 774, 882, 2688, 2991, 3547
BspQI	GCTCTTC	1 cut(s)	1878
BspT107I	GGYRCC	3 cut(s)	270, 1372, 1943
BspTNI	GGTCTC	1 cut(s)	900
BsrDI	GCAATG	2 cut(s)	1123, 1262
BsrFI	RCCGGY	3 cut(s)	1867, 3163, 3512
BsrI	ACTGG	7 cut(s)	613, 855, 1294, 2662, 3286, 3320, 3351
BssAI	RCCGGY	3 cut(s)	1867, 3163, 3512
BssECI	CCNNGG	3 cut(s)	487, 1205, 3482
BssNAI	GTATAC	1 cut(s)	1475
BssNI	GRCGYC	1 cut(s)	387
Bst1107I	GTATAC	1 cut(s)	1475
Bst2UI	CCWGG	5 cut(s)	489, 1206, 1634, 2053, 3484
Bst4CI	ACNGT	9 cut(s)	34, 1369, 1486, 1605, 2447, 2480, 2504, 2616, 2875
Bst6I	CTCTTC	5 cut(s)	1878, 1902, 2422, 2733, 3432
BstACI	GRCGYC	1 cut(s)	387
BstC8I	GCNNGC	8 cut(s)	269, 580, 922, 1562, 2299, 2421, 2547, 3165
BstDEI	CTNAG	8 cut(s)	946, 1556, 1578, 1662, 2261, 2302, 3362, 3429
BstEII	GGTNACC	2 cut(s)	600, 1931
BstF5I	GGATG	9 cut(s)	85, 217, 1081, 1328, 1840, 2061, 2139, 2734, 3541
BstFNI	CGCG	3 cut(s)	51, 177, 570
BstHHI	GCGC	4 cut(s)	379, 1007, 1541, 2061
BstMAI	GTCTC	5 cut(s)	653, 900, 2201, 3092, 3116
BstMWI	GCNNNNNNNGC	7 cut(s)	374, 1076, 1283, 1502, 2669, 2752, 3026
BstNI	CCWGG	5 cut(s)	489, 1206, 1634, 2053, 3484
BstNSI	RCATGY	4 cut(s)	287, 441, 1430, 3191
BstPI	GGTNACC	2 cut(s)	600, 1931
BstSCI	CCNGG	7 cut(s)	19, 129, 487, 1204, 1632, 2051, 3482
BstSFI	CTRYAG	4 cut(s)	1224, 1642, 1791, 2394
BstSLI	GKGCMC	3 cut(s)	1288, 1396, 1948
BstUI	CGCG	3 cut(s)	51, 177, 570
BstV1I	GCAGC	4 cut(s)	1489, 1747, 2025, 3179
BstX2I	RGATCY	6 cut(s)	462, 509, 766, 874, 2693, 2983
BstXI	CCANNNNNNTGG	2 cut(s)	1955, 3281
BstYI	RGATCY	6 cut(s)	462, 509, 766, 874, 2693, 2983
BstZ17I	GTATAC	1 cut(s)	1475
BsuI	GTATCC	3 cut(s)	1224, 1780, 2933
BsuRI	GGCC	7 cut(s)	157, 409, 476, 1041, 1564, 2534, 2790
BtsCI	GGATG	9 cut(s)	85, 217, 1081, 1328, 1840, 2061, 2139, 2734, 3541
BtsIMutI	CAGTG	7 cut(s)	848, 1586, 1601, 2567, 2880, 3279, 3393
BtuMI	TCGCGA	1 cut(s)	51
Cac8I	GCNNGC	8 cut(s)	269, 580, 922, 1562, 2299, 2421, 2547, 3165
CciI	TCATGA	1 cut(s)	3175
CfoI	GCGC	4 cut(s)	379, 1007, 1541, 2061
Cfr10I	RCCGGY	3 cut(s)	1867, 3163, 3512
Cfr13I	GGNCC	7 cut(s)	155, 172, 474, 819, 1202, 1259, 2532
CpoI	CGGWCCG	1 cut(s)	172
CseI	GACGC	2 cut(s)	576, 2044
Csp6I	GTAC	5 cut(s)	395, 995, 2238, 2255, 3331
CspAI	ACCGGT	1 cut(s)	3512
CspI	CGGWCCG	1 cut(s)	172
CviQI	GTAC	5 cut(s)	395, 995, 2238, 2255, 3331
DdeI	CTNAG	8 cut(s)	946, 1556, 1578, 1662, 2261, 2302, 3362, 3429
DraI	TTTAAA	2 cut(s)	1108, 1734
DrdI	GACNNNNNNGTC	1 cut(s)	3250
DseDI	GACNNNNNNGTC	1 cut(s)	3250
EaeI	YGGCCR	1 cut(s)	1562
Eam1104I	CTCTTC	5 cut(s)	1878, 1902, 2422, 2733, 3432
EarI	CTCTTC	5 cut(s)	1878, 1902, 2422, 2733, 3432
Eco31I	GGTCTC	1 cut(s)	900
Eco47I	GGWCC	4 cut(s)	172, 819, 1202, 1259
Eco57I	CTGAAG	4 cut(s)	239, 614, 2147, 3507
Eco91I	GGTNACC	2 cut(s)	600, 1931
EcoO109I	RGGNCCY	1 cut(s)	1202
EcoO65I	GGTNACC	2 cut(s)	600, 1931
EcoRII	CCWGG	5 cut(s)	487, 1204, 1632, 2051, 3482
EcoT22I	ATGCAT	3 cut(s)	2109, 2322, 2980
FalI	AAGNNNNNCTT	6 cut(s)	237, 269, 962, 994, 2169, 2201
FaqI	GGGAC	2 cut(s)	210, 568
FauI	CCCGC	2 cut(s)	925, 3017
FauNDI	CATATG	1 cut(s)	2763
FbaI	TGATCA	2 cut(s)	1852, 2758
FblI	GTMKAC	2 cut(s)	1474, 1806
Fnu4HI	GCNGC	6 cut(s)	477, 1503, 1761, 2039, 3168, 3467
FokI	GGATG	9 cut(s)	92, 204, 1088, 1315, 1827, 2068, 2126, 2741, 3548
Fsp4HI	GCNGC	6 cut(s)	477, 1503, 1761, 2039, 3168, 3467
FspAI	RTGCGCAY	1 cut(s)	1006
FspBI	CTAG	8 cut(s)	320, 575, 696, 900, 1824, 2424, 2945, 3396
FspI	TGCGCA	1 cut(s)	1006
GlaI	GCGC	4 cut(s)	378, 1006, 1540, 2060
GluI	GCNGC	6 cut(s)	477, 1503, 1761, 2039, 3168, 3467
GsaI	CCCAGC	2 cut(s)	928, 2586
GsuI	CTGGAG	4 cut(s)	526, 1862, 2403, 3491
HaeIII	GGCC	7 cut(s)	157, 409, 476, 1041, 1564, 2534, 2790
HapII	CCGG	9 cut(s)	21, 131, 170, 242, 303, 1868, 3164, 3404, 3513
HgaI	GACGC	2 cut(s)	576, 2044
HhaI	GCGC	4 cut(s)	379, 1007, 1541, 2061
Hin1I	GRCGYC	1 cut(s)	387
Hin6I	GCGC	4 cut(s)	377, 1005, 1539, 2059
HinP1I	GCGC	4 cut(s)	377, 1005, 1539, 2059
HincII	GTYRAC	3 cut(s)	693, 1807, 2994
HindII	GTYRAC	3 cut(s)	693, 1807, 2994
HindIII	AAGCTT	2 cut(s)	987, 3027
HpaI	GTTAAC	1 cut(s)	2994
HpaII	CCGG	9 cut(s)	21, 131, 170, 242, 303, 1868, 3164, 3404, 3513
HphI	GGTGA	7 cut(s)	594, 1925, 2325, 2890, 3131, 3487, 3511
Hpy99I	CGWCG	1 cut(s)	570
HpyAV	CCTTC	8 cut(s)	338, 343, 535, 805, 1152, 2284, 3439, 3566
HpyCH4III	ACNGT	9 cut(s)	34, 1369, 1486, 1605, 2447, 2480, 2504, 2616, 2875
HpyCH4IV	ACGT	2 cut(s)	387, 644
HpyF10VI	GCNNNNNNNGC	7 cut(s)	374, 1076, 1283, 1502, 2669, 2752, 3026
HpyF3I	CTNAG	8 cut(s)	946, 1556, 1578, 1662, 2261, 2302, 3362, 3429
HpySE526I	ACGT	2 cut(s)	387, 644
Hsp92I	GRCGYC	1 cut(s)	387
HspAI	GCGC	4 cut(s)	377, 1005, 1539, 2059
Kpn2I	TCCGGA	1 cut(s)	169
KroI	GCCGGC	1 cut(s)	3163
KroNI	GCCGGC	1 cut(s)	3165
Ksp22I	TGATCA	2 cut(s)	1852, 2758
KspAI	GTTAAC	1 cut(s)	2994
LguI	GCTCTTC	1 cut(s)	1878
LmnI	GCTCC	8 cut(s)	86, 190, 352, 755, 1689, 2579, 3011, 3044
Lsp1109I	GCAGC	4 cut(s)	1489, 1747, 2025, 3179
MaeI	CTAG	8 cut(s)	320, 575, 696, 900, 1824, 2424, 2945, 3396
MaeII	ACGT	2 cut(s)	387, 644
MaeIII	GTNAC	7 cut(s)	28, 237, 600, 1363, 1931, 3241, 3508
MfeI	CAATTG	1 cut(s)	3334
MflI	RGATCY	6 cut(s)	462, 509, 766, 874, 2693, 2983
MhlI	GDGCHC	5 cut(s)	1288, 1396, 1948, 3041, 3373
MlsI	TGGCCA	1 cut(s)	1564
MluNI	TGGCCA	1 cut(s)	1564
MlyI	GAGTC	6 cut(s)	175, 938, 1156, 1692, 3386, 3419
MmeI	TCCRAC	1 cut(s)	3033
Mox20I	TGGCCA	1 cut(s)	1564
Mph1103I	ATGCAT	3 cut(s)	2109, 2322, 2980
MroI	TCCGGA	1 cut(s)	169
MroNI	GCCGGC	1 cut(s)	3163
MscI	TGGCCA	1 cut(s)	1564
MslI	CAYNNNNRTG	7 cut(s)	282, 1520, 1953, 2315, 2973, 3239, 3284
Msp20I	TGGCCA	1 cut(s)	1564
MspA1I	CMGCKG	3 cut(s)	1760, 3170, 3469
MspI	CCGG	9 cut(s)	21, 131, 170, 242, 303, 1868, 3164, 3404, 3513
MspR9I	CCNGG	7 cut(s)	21, 131, 489, 1206, 1634, 2053, 3484
MunI	CAATTG	1 cut(s)	3334
Mva1269I	GAATGC	5 cut(s)	1609, 1628, 1686, 2107, 2320
MvaI	CCWGG	5 cut(s)	489, 1206, 1634, 2053, 3484
MvnI	CGCG	3 cut(s)	51, 177, 570
MwoI	GCNNNNNNNGC	7 cut(s)	374, 1076, 1283, 1502, 2669, 2752, 3026
NaeI	GCCGGC	1 cut(s)	3165
NciI	CCSGG	2 cut(s)	21, 131
NdeI	CATATG	1 cut(s)	2763
NgoMIV	GCCGGC	1 cut(s)	3163
NmuCI	GTSAC	6 cut(s)	28, 600, 1363, 1931, 3241, 3508
NruI	TCGCGA	1 cut(s)	51
NsbI	TGCGCA	1 cut(s)	1006
NsiI	ATGCAT	3 cut(s)	2109, 2322, 2980
NspI	RCATGY	4 cut(s)	287, 441, 1430, 3191
OliI	CACNNNNGTG	1 cut(s)	3239
PacI	TTAATTAA	1 cut(s)	2889
PagI	TCATGA	1 cut(s)	3175
PciI	ACATGT	2 cut(s)	283, 1426
PciSI	GCTCTTC	1 cut(s)	1878
PctI	GAATGC	5 cut(s)	1609, 1628, 1686, 2107, 2320
PdiI	GCCGGC	1 cut(s)	3165
PfeI	GAWTC	7 cut(s)	109, 117, 338, 680, 2572, 2596, 2629
PflMI	CCANNNNNTGG	1 cut(s)	416
PinAI	ACCGGT	1 cut(s)	3512
PkrI	GCNGC	6 cut(s)	478, 1504, 1762, 2040, 3169, 3468
PleI	GAGTC	6 cut(s)	174, 938, 1156, 1692, 3386, 3418
PpsI	GAGTC	6 cut(s)	174, 938, 1156, 1692, 3386, 3418
PpuMI	RGGWCCY	1 cut(s)	1202
PscI	ACATGT	2 cut(s)	283, 1426
PshBI	ATTAAT	2 cut(s)	1184, 1833
PsiI	TTATAA	2 cut(s)	2457, 2667
Psp1406I	AACGTT	1 cut(s)	644
Psp5II	RGGWCCY	1 cut(s)	1202
Psp6I	CCWGG	5 cut(s)	487, 1204, 1632, 2051, 3482
PspEI	GGTNACC	2 cut(s)	600, 1931
PspFI	CCCAGC	2 cut(s)	924, 2582
PspGI	CCWGG	5 cut(s)	487, 1204, 1632, 2051, 3482
PspPI	GGNCC	7 cut(s)	155, 172, 474, 819, 1202, 1259, 2532
PspPPI	RGGWCCY	1 cut(s)	1202
PstI	CTGCAG	1 cut(s)	2398
PsuI	RGATCY	6 cut(s)	462, 509, 766, 874, 2693, 2983
PvuII	CAGCTG	1 cut(s)	3170
RruI	TCGCGA	1 cut(s)	51
RsaI	GTAC	5 cut(s)	396, 996, 2239, 2256, 3332
RsaNI	GTAC	5 cut(s)	395, 995, 2238, 2255, 3331
RseI	CAYNNNNRTG	7 cut(s)	282, 1520, 1953, 2315, 2973, 3239, 3284
Rsr2I	CGGWCCG	1 cut(s)	172
RsrII	CGGWCCG	1 cut(s)	172
SalI	GTCGAC	1 cut(s)	1805
SapI	GCTCTTC	1 cut(s)	1878
SatI	GCNGC	6 cut(s)	477, 1503, 1761, 2039, 3168, 3467
Sau96I	GGNCC	7 cut(s)	155, 172, 474, 819, 1202, 1259, 2532
SchI	GAGTC	6 cut(s)	175, 938, 1156, 1692, 3386, 3419
ScrFI	CCNGG	7 cut(s)	21, 131, 489, 1206, 1634, 2053, 3484
SduI	GDGCHC	5 cut(s)	1288, 1396, 1948, 3041, 3373
SfcI	CTRYAG	4 cut(s)	1224, 1642, 1791, 2394
SinI	GGWCC	4 cut(s)	172, 819, 1202, 1259
SmiMI	CAYNNNNRTG	7 cut(s)	282, 1520, 1953, 2315, 2973, 3239, 3284
SmlI	CTYRAG	3 cut(s)	741, 1700, 1970
SmoI	CTYRAG	3 cut(s)	741, 1700, 1970
SpeI	ACTAGT	1 cut(s)	695
SsiI	CCGC	9 cut(s)	175, 477, 492, 570, 627, 918, 1758, 3010, 3467
SspI	AATATT	2 cut(s)	2461, 3325
SspMI	CTAG	8 cut(s)	320, 575, 696, 900, 1824, 2424, 2945, 3396
StyD4I	CCNGG	7 cut(s)	19, 129, 487, 1204, 1632, 2051, 3482
TaaI	ACNGT	9 cut(s)	34, 1369, 1486, 1605, 2447, 2480, 2504, 2616, 2875
TaiI	ACGT	2 cut(s)	390, 647
TaqI	TCGA	6 cut(s)	325, 565, 1021, 1445, 1806, 2949
TatI	WGTACW	2 cut(s)	994, 2254
TauI	GCSGC	2 cut(s)	479, 3469
TfiI	GAWTC	7 cut(s)	109, 117, 338, 680, 2572, 2596, 2629
TscAI	CASTG	7 cut(s)	855, 1586, 1608, 2574, 2880, 3286, 3393
TseFI	GTSAC	6 cut(s)	28, 600, 1363, 1931, 3241, 3508
TseI	GCWGC	4 cut(s)	1502, 1760, 2038, 3167
Tsp45I	GTSAC	6 cut(s)	28, 600, 1363, 1931, 3241, 3508
TspGWI	ACGGA	4 cut(s)	16, 117, 3153, 3208
TspRI	CASTG	7 cut(s)	855, 1586, 1608, 2574, 2880, 3286, 3393
Van91I	CCANNNNNTGG	1 cut(s)	416
VneI	GTGCAC	1 cut(s)	1392
VpaK11BI	GGWCC	4 cut(s)	172, 819, 1202, 1259
VspI	ATTAAT	2 cut(s)	1184, 1833
XapI	RAATTY	6 cut(s)	632, 1409, 2641, 2850, 2891, 3146
XceI	RCATGY	4 cut(s)	287, 441, 1430, 3191
XmiI	GTMKAC	2 cut(s)	1474, 1806
XspI	CTAG	8 cut(s)	320, 575, 696, 900, 1824, 2424, 2945, 3396
ZraI	GACGTC	1 cut(s)	388
Zsp2I	ATGCAT	3 cut(s)	2109, 2322, 2980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.