Rroxscaffold_4G00324970

cell division cycle protein 48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018148
Physical Location & Seq
Reverse (-)
56662692 .. 56663309
618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324970.1

Sequence Viewer

Length: 489 bp
ATGTGGTATGTTTTTCCCAGCCTCGTGGTGTCCTCTTCTTTGATGTGCTCGATTCCATTGCTACTCAAAGAGGTAGCAAGTGTAGGTGTTGATAGGGTTTTGAACATTACAAAACAAGATGAAGTGTCTACCAAGAAAAGTATTTTCATTATCGGCAGCCCTAACGGACTCGACCTTATAGAACCTGCACTTCTACATCCAAGCACATCTGGCCCCAATGAGGAATCATACTTGAATATTCAAGGCTTGCCCGTTTGCTTGAGGAAATCACCGCCGGGGAGATCACGGAAGCTAATTTTGAAGGATCAAGAGTATATTCACCGGATTGTTAATGATTCGATATCCTCAAGCATCATGCAGCGCAGTACATTGCACCAGGTGAGATGGTTTGGATCAGACCAATTTAGGTTTAAGATTTTGGGCCCGCTATTTGATCCGATCCCCTTACTACTACCGCCGATAGAGCTACTAAAGATGACTCTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

162

Amino Acids

18.19

Weight (kDa)

8.97

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 193
AccI GTMKAC 1 cut(s) 128
AciI CCGC 3 cut(s) 272, 425, 455
AclWI GGATC 4 cut(s) 312, 400, 428, 433
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 220
AgsI TTSAA 4 cut(s) 103, 235, 242, 301
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 2 cut(s) 292, 466
AluI AGCT 2 cut(s) 292, 466
Alw21I GWGCWC 1 cut(s) 50
AlwI GGATC 4 cut(s) 312, 400, 428, 433
AoxI GGCC 2 cut(s) 211, 421
ApaI GGGCCC 1 cut(s) 425
ApeKI GCWGC 2 cut(s) 156, 358
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 212, 421, 422
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 3 cut(s) 261, 311, 391
BaeGI GKGCMC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 425
BauI CACGAG 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 168, 370
BccI CCATC 1 cut(s) 378
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 2 cut(s) 157, 359
BlsI GCNGC 2 cut(s) 158, 360
Bme1390I CCNGG 2 cut(s) 276, 377
BmgT120I GGNCC 3 cut(s) 212, 421, 422
BmiI GGNNCC 2 cut(s) 214, 423
BmrFI CCNGG 2 cut(s) 276, 377
BmsI GCATC 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 280, 331
BpuMI CCSGG 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 275
BsaWI WCCGGW 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse3DI GCAATG 2 cut(s) 56, 368
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMI GCAATG 2 cut(s) 56, 368
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 2 cut(s) 168, 370
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 2 cut(s) 213, 423
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 275, 322
BslI CCNNNNNNNGG 1 cut(s) 220
BsnI GGCC 2 cut(s) 213, 423
Bsp120I GGGCCC 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 50, 425
Bsp143I GATC 5 cut(s) 281, 304, 392, 433, 438
BspACI CCGC 3 cut(s) 272, 425, 455
BspANI GGCC 2 cut(s) 213, 423
BspLI GGNNCC 2 cut(s) 214, 423
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 4 cut(s) 312, 400, 428, 433
BsrDI GCAATG 2 cut(s) 56, 368
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 5 cut(s) 281, 304, 392, 433, 438
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 486
Bst6I CTCTTC 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 248, 425
BstF5I GGATG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 5 cut(s) 284, 307, 395, 436, 441
BstMBI GATC 5 cut(s) 281, 304, 392, 433, 438
BstMWI GCNNNNNNNGC 2 cut(s) 210, 463
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 274, 375
BstSFI CTRYAG 1 cut(s) 482
BstSLI GKGCMC 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 168, 370
BstXI CCANNNNNNTGG 1 cut(s) 25
BsuRI GGCC 2 cut(s) 213, 423
BtsCI GGATG 1 cut(s) 196
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 248, 425
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 3 cut(s) 212, 421, 422
CsiI ACCWGGT 1 cut(s) 375
Csp6I GTAC 1 cut(s) 366
CviAII CATG 1 cut(s) 355
CviJI RGCY 7 cut(s) 21, 159, 213, 246, 292, 423, 466
CviKI_1 RGCY 7 cut(s) 21, 159, 213, 246, 292, 423, 466
CviQI GTAC 1 cut(s) 366
DpnI GATC 5 cut(s) 283, 306, 394, 435, 440
DpnII GATC 5 cut(s) 281, 304, 392, 433, 438
DraIII CACNNNGTG 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 425
Eco32I GATATC 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 375
EcoRV GATATC 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 425
FaeI CATG 1 cut(s) 358
FaiI YATR 5 cut(s) 9, 179, 229, 315, 356
FatI CATG 1 cut(s) 354
FauI CCCGC 1 cut(s) 432
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 2 cut(s) 157, 359
FokI GGATG 1 cut(s) 183
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 2 cut(s) 157, 359
GlaI GCGC 1 cut(s) 362
GluI GCNGC 2 cut(s) 157, 359
GsaI CCCAGC 1 cut(s) 21
HaeIII GGCC 2 cut(s) 213, 423
HapII CCGG 2 cut(s) 275, 322
HhaI GCGC 1 cut(s) 363
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HinfI GANTC 5 cut(s) 52, 168, 224, 335, 478
HpaII CCGG 2 cut(s) 275, 322
HphI GGTGA 3 cut(s) 261, 311, 391
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 2 cut(s) 397, 438
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 129
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4V TGCA 3 cut(s) 188, 358, 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 463
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 5 cut(s) 281, 304, 392, 433, 438
LpnPI CCDG 7 cut(s) 31, 195, 198, 288, 335, 362, 389
Lsp1109I GCAGC 2 cut(s) 168, 370
LweI GCATC 1 cut(s) 360
MabI ACCWGGT 1 cut(s) 375
MalI GATC 5 cut(s) 283, 306, 394, 435, 440
MboI GATC 5 cut(s) 281, 304, 392, 433, 438
MboII GAAGA 1 cut(s) 27
MhlI GDGCHC 2 cut(s) 50, 425
MluCI AATT 2 cut(s) 294, 401
MlyI GAGTC 2 cut(s) 162, 472
MnlI CCTC 6 cut(s) 32, 43, 64, 214, 255, 355
MseI TTAA 2 cut(s) 330, 411
MspI CCGG 2 cut(s) 275, 322
MspR9I CCNGG 2 cut(s) 276, 377
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 2 cut(s) 210, 463
NciI CCSGG 1 cut(s) 276
NdeII GATC 5 cut(s) 281, 304, 392, 433, 438
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 2 cut(s) 214, 423
PfeI GAWTC 3 cut(s) 52, 224, 335
PkrI GCNGC 2 cut(s) 158, 360
PleI GAGTC 2 cut(s) 162, 472
PpsI GAGTC 2 cut(s) 162, 472
Psp6I CCWGG 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 2 cut(s) 214, 423
PspOMI GGGCCC 1 cut(s) 421
PspPI GGNCC 3 cut(s) 212, 421, 422
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SaqAI TTAA 2 cut(s) 330, 411
SatI GCNGC 2 cut(s) 157, 359
Sau3AI GATC 5 cut(s) 281, 304, 392, 433, 438
Sau96I GGNCC 3 cut(s) 212, 421, 422
SchI GAGTC 2 cut(s) 162, 472
ScrFI CCNGG 2 cut(s) 276, 377
SduI GDGCHC 2 cut(s) 50, 425
SetI ASST 8 cut(s) 75, 88, 177, 187, 294, 381, 410, 468
SexAI ACCWGGT 1 cut(s) 375
SfaNI GCATC 1 cut(s) 360
SfcI CTRYAG 1 cut(s) 482
SmlI CTYRAG 2 cut(s) 259, 346
SmoI CTYRAG 2 cut(s) 259, 346
Sse9I AATT 2 cut(s) 294, 401
SsiI CCGC 3 cut(s) 272, 425, 455
SspI AATATT 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 274, 375
TaaI ACNGT 1 cut(s) 486
TaqI TCGA 3 cut(s) 50, 171, 338
TasI AATT 2 cut(s) 294, 401
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 3 cut(s) 52, 224, 335
Tru1I TTAA 2 cut(s) 330, 411
Tru9I TTAA 2 cut(s) 330, 411
TseI GCWGC 2 cut(s) 156, 358
TspDTI ATGAA 2 cut(s) 135, 136
TspGWI ACGGA 2 cut(s) 180, 301
XmiI GTMKAC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.