Rroxscaffold_5G00387190

Mitosis protein DIM1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004652
Physical Location & Seq
Forward (+)
65909404 .. 65910813
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387190.1

Sequence Viewer

Length: 429 bp
ATGTCGTATTTGCTGCCGCACCTGCACTCAGGATGGGCCGTCGACCAGGCGATTCTGGCCGAGGAAGAACGTGTTGTCATCATACGATTCGGCCATGATTGGGACGAGACTTGCATGCAGATGGATGAAGTGCTAGCATCAGTGGCTGACAAAATTAAAAACTTTGCTGTCATTTACCTTGTGGACATTTCAGAGGTTCCTGATTTCAACACAATGTATGAGCTGTATGACCCATCCACAGTTATGTTCTTTTTCAGAAACAAGCACATTATGATTGACCTTGGCACTGGGAACAACAACAAGATTAACTGGGCTCTCAAGGACAAGCAAGAGTTCATTGACATTGTGGAGACAGTGTACCGTGGTGCAAGGAAGGGGCGTGGTCTGGTGATTGCTCCCAAGGATTACTCCACCAAGTACCGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DIM1

Protein Analysis

142

Amino Acids

16.56

Weight (kDa)

5.31

Isoelectric Point (pI)

21.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIM1 PF02966 4 - 136 6.7e-69 Mitosis protein DIM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 30
Acc36I ACCTGC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 42
AciI CCGC 2 cut(s) 17, 421
AcoI YGGCCR 2 cut(s) 57, 91
AfaI GTAC 2 cut(s) 359, 419
AfiI CCNNNNNNNGG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 70
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 1 cut(s) 223
AluI AGCT 1 cut(s) 223
Alw26I GTCTC 2 cut(s) 101, 344
AlwNI CAGNNNCTG 1 cut(s) 146
AoxI GGCC 3 cut(s) 36, 57, 91
ApeKI GCWGC 1 cut(s) 13
AspS9I GGNCC 1 cut(s) 36
AsuHPI GGTGA 1 cut(s) 400
AsuNHI GCTAGC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 316
BccI CCATC 3 cut(s) 27, 115, 241
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 47
BcoDI GTCTC 2 cut(s) 101, 344
BfaI CTAG 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 30
BisI GCNGC 2 cut(s) 14, 17
BlsI GCNGC 2 cut(s) 15, 18
Bme1390I CCNGG 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 2 cut(s) 297, 319
BmsI GCATC 1 cut(s) 146
BmtI GCTAGC 1 cut(s) 137
BmuI ACTGGG 2 cut(s) 297, 319
BpuEI CTTGAG 1 cut(s) 302
BsaJI CCNNGG 4 cut(s) 60, 280, 361, 399
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 292, 314
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 4 cut(s) 60, 280, 361, 399
BseGI GGATG 3 cut(s) 38, 130, 233
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 292, 314
BsgI GTGCAG 1 cut(s) 8
BshFI GGCC 3 cut(s) 38, 59, 93
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 2 cut(s) 101, 344
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 3 cut(s) 38, 59, 93
Bsp1286I GDGCHC 1 cut(s) 316
BspACI CCGC 2 cut(s) 17, 421
BspANI GGCC 3 cut(s) 38, 59, 93
BspCNI CTCAG 1 cut(s) 41
BspLI GGNNCC 1 cut(s) 198
BspMI ACCTGC 1 cut(s) 30
BspOI GCTAGC 1 cut(s) 137
BsrI ACTGG 2 cut(s) 292, 314
BssECI CCNNGG 4 cut(s) 60, 280, 361, 399
BssT1I CCWWGG 2 cut(s) 280, 399
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 3 cut(s) 241, 355, 362
BstC8I GCNNGC 2 cut(s) 116, 135
BstDEI CTNAG 1 cut(s) 28
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 3 cut(s) 38, 130, 233
BstMAI GTCTC 2 cut(s) 101, 344
BstMWI GCNNNNNNNGC 3 cut(s) 22, 56, 143
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 45
BsuRI GGCC 3 cut(s) 38, 59, 93
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 3 cut(s) 38, 130, 233
BtsIMutI CAGTG 3 cut(s) 147, 285, 360
BveI ACCTGC 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 116, 135
CaiI CAGNNNCTG 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 36
Csp6I GTAC 2 cut(s) 358, 418
CviAII CATG 2 cut(s) 95, 115
CviJI RGCY 6 cut(s) 38, 59, 93, 146, 223, 314
CviKI_1 RGCY 6 cut(s) 38, 59, 93, 146, 223, 314
CviQI GTAC 2 cut(s) 358, 418
DdeI CTNAG 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 57, 91
Eco130I CCWWGG 2 cut(s) 280, 399
Eco24I GRGCYC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 280, 399
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 2 cut(s) 280, 399
FaeI CATG 2 cut(s) 98, 118
FaiI YATR 7 cut(s) 83, 96, 116, 219, 228, 245, 272
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 94, 114
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 14, 17
FokI GGATG 3 cut(s) 45, 137, 220
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 2 cut(s) 14, 17
FspBI CTAG 1 cut(s) 134
GluI GCNGC 2 cut(s) 14, 17
HaeIII GGCC 3 cut(s) 38, 59, 93
Hin1II CATG 2 cut(s) 98, 118
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 2 cut(s) 52, 87
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 3 cut(s) 43, 184, 358
Hpy188I TCNGA 2 cut(s) 193, 257
Hpy188III TCNNGA 2 cut(s) 30, 200
Hpy8I GTNNAC 3 cut(s) 43, 184, 358
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 3 cut(s) 241, 355, 362
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 4 cut(s) 25, 114, 118, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 56, 143
HpyF3I CTNAG 1 cut(s) 28
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 2 cut(s) 98, 118
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 9 cut(s) 15, 32, 35, 41, 59, 213, 273, 295, 371
LweI GCATC 1 cut(s) 146
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 70
MboII GAAGA 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 1 cut(s) 153
MnlI CCTC 2 cut(s) 55, 187
MseI TTAA 2 cut(s) 156, 306
MslI CAYNNNNRTG 2 cut(s) 119, 242
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
MwoI GCNNNNNNNGC 3 cut(s) 22, 56, 143
NheI GCTAGC 1 cut(s) 133
NlaIII CATG 2 cut(s) 98, 118
NlaIV GGNNCC 1 cut(s) 198
NmeAIII GCCGAG 1 cut(s) 85
NspI RCATGY 1 cut(s) 118
PaeI GCATGC 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 30
PfeI GAWTC 2 cut(s) 52, 87
PkrI GCNGC 2 cut(s) 15, 18
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 36
PstNI CAGNNNCTG 1 cut(s) 146
RsaI GTAC 2 cut(s) 359, 419
RsaNI GTAC 2 cut(s) 358, 418
RseI CAYNNNNRTG 2 cut(s) 119, 242
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 156, 306
SatI GCNGC 2 cut(s) 14, 17
Sau96I GGNCC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 316
SetI ASST 6 cut(s) 24, 73, 180, 198, 225, 282
SfaNI GCATC 1 cut(s) 146
SmiMI CAYNNNNRTG 2 cut(s) 119, 242
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 1 cut(s) 118
Sse9I AATT 1 cut(s) 153
SsiI CCGC 2 cut(s) 17, 421
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 2 cut(s) 280, 399
TaaI ACNGT 3 cut(s) 241, 355, 362
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 153
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 2 cut(s) 52, 87
Tru1I TTAA 2 cut(s) 156, 306
Tru9I TTAA 2 cut(s) 156, 306
TscAI CASTG 3 cut(s) 147, 292, 360
TseI GCWGC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 141, 325
TspRI CASTG 3 cut(s) 147, 292, 360
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.