Rroxscaffold_6G00414140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007210
Physical Location & Seq
Reverse (-)
36448729 .. 36452152
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414140.1

Sequence Viewer

Length: 750 bp
ATGCCAGCGGCAGTCTCCCTGATCTCAAGCCCTTCACCTCCGCAGAAGCTCTTCAAGACCGCTCGGATTTCCTACTCCGGCGAATCCGACTCCAAATCGGAGGGAGAGGTCGTCGAGGAGCCTTGGGCTTTCCACCCGCCGAGGAAGATCGCCAGGTCATCAAACGACTGCAATTCTCTCGCCGTAGTCAGAAATGTGCCACGCACGCCTACCACGCCGGTGTACGCTTCGCCGGAGTCGCATTTGTCGCCGGGAATCGGGAGGTTTAGTAGTCCTGTTAGCTATCCGCAGAGAGAAGAAAGGGATAATAATCCTGTGCAGACGGTGCAGCATAGCTTGTCTGGGATGTTTCCTTCGGTTTCTTTGTTTAAGGAGTATCAAGATGCAGCTATGGCGATCTTGGAGAAAACTGATTACACTAAGATTCATGGGAGTACTTATATCAAAAAGTCAGGTTGGAGGAAGATATCGTTTTACTTCAATCTCTCTTTTGAGATCAAGGACAAGAGCATTGAGTTTGATGAGAGTCGAAATGTTCTGCGTGCAGAGTTTGTGGTTCGGGCACACATGCAGGGTGGTAGGTTCTCGGATGGATGGGGCTCATGTGATCGGCGTGAGAAGAGATTCAACAAACCAAATCATGATATTCCAAGTACAGCAGAGACCAGGGCGAAAAATAAAGCATCCTCGGACTTGCTAGGAATTGGAGATTACAGACCTGGTGCAAGTCAAGACCACCAATATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.91

Weight (kDa)

9.11

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 62
AciI CCGC 5 cut(s) 8, 41, 60, 137, 287
AfaI GTAC 3 cut(s) 224, 436, 655
AfiI CCNNNNNNNGG 1 cut(s) 257
AgsI TTSAA 3 cut(s) 55, 481, 628
AjnI CCWGG 3 cut(s) 152, 665, 718
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 4 cut(s) 49, 282, 336, 389
AluI AGCT 4 cut(s) 49, 282, 336, 389
Alw26I GTCTC 2 cut(s) 19, 656
ApeKI GCWGC 2 cut(s) 328, 386
Asp700I GAANNNNTTC 2 cut(s) 50, 623
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 602
BbvI GCAGC 2 cut(s) 340, 398
BccI CCATC 2 cut(s) 584, 588
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 3 cut(s) 154, 667, 720
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 19, 656
BfaI CTAG 1 cut(s) 698
BisI GCNGC 3 cut(s) 9, 329, 387
BlsI GCNGC 3 cut(s) 10, 330, 388
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 4 cut(s) 154, 252, 667, 720
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 4 cut(s) 154, 252, 667, 720
BmsI GCATC 2 cut(s) 373, 692
BpuEI CTTGAG 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 4 cut(s) 122, 140, 666, 687
BsaXI ACNNNNNCTCC 4 cut(s) 96, 126, 253, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse118I RCCGGY 1 cut(s) 217
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 3 cut(s) 154, 667, 720
BseDI CCNNGG 4 cut(s) 122, 140, 666, 687
BseGI GGATG 4 cut(s) 351, 595, 599, 683
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 2 cut(s) 340, 398
BsgI GTGCAG 3 cut(s) 338, 347, 564
BsiSI CCGG 4 cut(s) 78, 218, 233, 251
BslI CCNNNNNNNGG 1 cut(s) 257
BsmAI GTCTC 2 cut(s) 19, 656
Bso31I GGTCTC 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 565, 602
Bsp143I GATC 5 cut(s) 21, 147, 396, 495, 607
BspACI CCGC 5 cut(s) 8, 41, 60, 137, 287
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 1 cut(s) 120
BspQI GCTCTTC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 4 cut(s) 122, 140, 666, 687
BssMI GATC 5 cut(s) 21, 147, 396, 495, 607
BssT1I CCWWGG 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 154, 667, 720
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 2 cut(s) 56, 614
BstAPI GCANNNNNTGC 1 cut(s) 325
BstC8I GCNNGC 3 cut(s) 6, 206, 543
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 4 cut(s) 351, 595, 599, 683
BstKTI GATC 5 cut(s) 24, 150, 399, 498, 610
BstMAI GTCTC 2 cut(s) 19, 656
BstMBI GATC 5 cut(s) 21, 147, 396, 495, 607
BstMWI GCNNNNNNNGC 6 cut(s) 205, 214, 238, 247, 325, 392
BstNI CCWGG 3 cut(s) 154, 667, 720
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 4 cut(s) 152, 250, 665, 718
BstSLI GKGCMC 1 cut(s) 565
BstV1I GCAGC 2 cut(s) 340, 398
BtsCI GGATG 4 cut(s) 351, 595, 599, 683
Cac8I GCNNGC 3 cut(s) 6, 206, 543
CciI TCATGA 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 217
CsiI ACCWGGT 1 cut(s) 718
Csp6I GTAC 3 cut(s) 223, 435, 654
CviAII CATG 5 cut(s) 428, 568, 603, 641, 747
CviJI RGCY 8 cut(s) 30, 49, 121, 128, 282, 336, 389, 600
CviKI_1 RGCY 8 cut(s) 30, 49, 121, 128, 282, 336, 389, 600
CviQI GTAC 3 cut(s) 223, 435, 654
DdeI CTNAG 1 cut(s) 420
DpnI GATC 5 cut(s) 23, 149, 398, 497, 609
DpnII GATC 5 cut(s) 21, 147, 396, 495, 607
Eam1104I CTCTTC 2 cut(s) 56, 614
EarI CTCTTC 2 cut(s) 56, 614
Eco130I CCWWGG 1 cut(s) 122
Eco24I GRGCYC 1 cut(s) 602
Eco31I GGTCTC 1 cut(s) 656
Eco32I GATATC 1 cut(s) 468
EcoRII CCWGG 3 cut(s) 152, 665, 718
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 122
EcoT22I ATGCAT 1 cut(s) 748
EcoT38I GRGCYC 1 cut(s) 602
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 5 cut(s) 431, 571, 606, 644, 750
FaiI YATR 9 cut(s) 333, 392, 429, 441, 569, 604, 642, 744, 748
FatI CATG 5 cut(s) 427, 567, 602, 640, 746
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 3 cut(s) 9, 329, 387
FokI GGATG 4 cut(s) 358, 602, 606, 670
FriOI GRGCYC 1 cut(s) 602
Fsp4HI GCNGC 3 cut(s) 9, 329, 387
FspBI CTAG 1 cut(s) 698
GluI GCNGC 3 cut(s) 9, 329, 387
HapII CCGG 4 cut(s) 78, 218, 233, 251
Hin1II CATG 5 cut(s) 431, 571, 606, 644, 750
HinfI GANTC 7 cut(s) 83, 89, 236, 255, 424, 526, 624
HpaII CCGG 4 cut(s) 78, 218, 233, 251
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 6 cut(s) 66, 88, 100, 191, 589, 691
Hpy188III TCNNGA 5 cut(s) 55, 259, 380, 641, 731
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 42, 363
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 8 cut(s) 171, 319, 328, 386, 545, 571, 725, 746
HpyF10VI GCNNNNNNNGC 6 cut(s) 205, 214, 238, 247, 325, 392
HpyF3I CTNAG 1 cut(s) 420
Hsp92II CATG 5 cut(s) 431, 571, 606, 644, 750
Kzo9I GATC 5 cut(s) 21, 147, 396, 495, 607
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 2 cut(s) 340, 398
LweI GCATC 2 cut(s) 373, 692
MabI ACCWGGT 1 cut(s) 718
MaeI CTAG 1 cut(s) 698
MalI GATC 5 cut(s) 23, 149, 398, 497, 609
MbiI CCGCTC 1 cut(s) 62
MboI GATC 5 cut(s) 21, 147, 396, 495, 607
MboII GAAGA 5 cut(s) 43, 157, 308, 475, 631
MhlI GDGCHC 2 cut(s) 565, 602
MluCI AATT 2 cut(s) 172, 702
MlyI GAGTC 3 cut(s) 83, 245, 535
MmeI TCCRAC 2 cut(s) 111, 437
MnlI CCTC 8 cut(s) 48, 94, 100, 109, 135, 255, 453, 697
Mph1103I ATGCAT 1 cut(s) 748
MroXI GAANNNNTTC 2 cut(s) 50, 623
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 2 cut(s) 218, 741
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 4 cut(s) 78, 218, 233, 251
MspR9I CCNGG 4 cut(s) 154, 252, 667, 720
MvaI CCWGG 3 cut(s) 154, 667, 720
MwoI GCNNNNNNNGC 6 cut(s) 205, 214, 238, 247, 325, 392
NciI CCSGG 1 cut(s) 252
NdeII GATC 5 cut(s) 21, 147, 396, 495, 607
NlaIII CATG 5 cut(s) 431, 571, 606, 644, 750
NlaIV GGNNCC 1 cut(s) 120
NmeAIII GCCGAG 1 cut(s) 165
NsiI ATGCAT 1 cut(s) 748
NspI RCATGY 1 cut(s) 571
OliI CACNNNNGTG 1 cut(s) 218
PagI TCATGA 1 cut(s) 640
PciSI GCTCTTC 1 cut(s) 56
PdmI GAANNNNTTC 2 cut(s) 50, 623
PfeI GAWTC 4 cut(s) 83, 255, 424, 624
PkrI GCNGC 3 cut(s) 10, 330, 388
PleI GAGTC 3 cut(s) 83, 244, 534
PpsI GAGTC 3 cut(s) 83, 244, 534
Psp6I CCWGG 3 cut(s) 152, 665, 718
PspGI CCWGG 3 cut(s) 152, 665, 718
PspN4I GGNNCC 1 cut(s) 120
RsaI GTAC 3 cut(s) 224, 436, 655
RsaNI GTAC 3 cut(s) 223, 435, 654
RseI CAYNNNNRTG 2 cut(s) 218, 741
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 9, 329, 387
Sau3AI GATC 5 cut(s) 21, 147, 396, 495, 607
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 3 cut(s) 83, 245, 535
ScrFI CCNGG 4 cut(s) 154, 252, 667, 720
SduI GDGCHC 2 cut(s) 565, 602
SexAI ACCWGGT 1 cut(s) 718
SfaNI GCATC 2 cut(s) 373, 692
SgrAI CRCCGGYG 1 cut(s) 217
SmiMI CAYNNNNRTG 2 cut(s) 218, 741
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 2 cut(s) 172, 702
SsiI CCGC 5 cut(s) 8, 41, 60, 137, 287
SspMI CTAG 1 cut(s) 698
StyD4I CCNGG 4 cut(s) 152, 250, 665, 718
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 114, 529
TasI AATT 2 cut(s) 172, 702
TatI WGTACW 2 cut(s) 434, 653
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 4 cut(s) 83, 255, 424, 624
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseI GCWGC 2 cut(s) 328, 386
TspDTI ATGAA 1 cut(s) 416
XceI RCATGY 1 cut(s) 571
XmnI GAANNNNTTC 2 cut(s) 50, 623
XspI CTAG 1 cut(s) 698
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.