Rroxscaffold_7G00165390

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006598
Physical Location & Seq
Forward (+)
7148912 .. 7152834
3923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00165390.1

Sequence Viewer

Length: 1236 bp
ATGGAGGATCCGAGTCGCCTACCTCGCCCGACTCAATTCCAGTACCATCGTCTACATTCTCACTCGCTCCAGGCTCCGACCTCATCATCTCTGCCGTCGAATCCACGCCGTGTTCGCCACTTCACCCCGCCGCCGGACGATGAAGATTCCAAACCTTTAAGCCTCTCCGATAAGCCTCTCAGTTCACGCGCCGACGCCTACGCCGCTTTTTCTAAGCTTGAGTTGGGGAGGATTAAAGAAGAAGCGGATGACTGTGAGGCCCAGACTAGTGGGCAGGCAACTGCTGCTGACCAAAAGAAAGTACTTAATGATCCTGTATCCACTATACCAAAGTCCTGGAGAGGTGTACAGAAGATCAGTAAATCAAAGGTTGCTAAGCCTATCAAATCTGAGAATGTAAATGCAGAGTCTCAACAAAATGGCTTGACTCCAGTTACTACTTGCCGTTTTGACAGTTCTTTAGGCTTGTTAACTAAGAAATTCATCAGATTACTTCAGGATACCAAGGATAGAACTCTTGATTTAAACAAAGCAGCAAAGGTGTTGGAGGTTCAAAAGAGGAGGATATATGATATTACAAACGTTCTTGAAGGAATAAATTTGTTAGTAAAAGTTTCAAAGAACCACGTCCGCTGGAAGGGACATGATGCGCCAGGGGAACTGGATGATCAAGTTTTTAAATTAAAGGAGGAGGTTGAATCCTTATGTGCTGAAGAATGCAGGCTTAATGACGCTATAAGGGAAAGACGAGAGCTTATAAGGACTGTGAAGGAAGATAGGAACAATAAAAAGTACCTCTTTTTGACGGAGGGAGATATCTTGAGCCTTCCCTGCTTCCAGAATCAAACTCTCATTGCAATAAAAGCTCCTCATGCTAGTACTATTGAAGTTCTAGATCCTGATGAGGATATTGATTTTCCCCAAGGACAGTACAGAATGACGGTCAGAAGCGCCACAGGACAGATAGATTGTTACCTCTTAAGTAATTACCAAGGCCAACAAGAAGGCATTACCGTTAAGCAAGAACACTCAACAGGTTCATCTCCTTGCTATAATAACACTGGCTATTGCAGAATGGAAGATGGAGGGATGTCACCAGAAATTCATAGCTTACCAGGCACAGAGGCATCTTCTGGGATGCAGAAGATTGTTCTTTCGCACTTGGATGCCAACGATGACTATTGGTTTAGATCAGAACCTGAAGTCAGCCTTACAGATCTCTGGGCAACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006082 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0022402 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032502 GO:0032989 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0046483 GO:0046982 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051301 GO:0051302 GO:0051782 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140110 GO:1901135 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1901657 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01522 ko04110 ko04218 ko04934 ko05161 ko05166 ko05167 ko05200 ko05206 ko05212 ko05214 ko05215 ko05218 ko05219 ko05220 ko05222 ko05223 ko05224 ko05225 ko05226 map01522 map04110 map04218 map04934 map05161 map05166 map05167 map05200 map05206 map05212 map05214 map05215 map05218 map05219 map05220 map05222 map05223 map05224 map05225 map05226
Pfam Domains
Protein Families
E2F_CC-MB E2F_TDP

Protein Analysis

411

Amino Acids

46.24

Weight (kDa)

5.99

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_E2F_TDP PF02319 151 - 214 1.2e-25 E2F/DP family winged-helix DNA-binding domain
E2F_CC-MB PF16421 228 - 328 1.7e-24 E2F transcription factor CC-MB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
AccI GTMKAC 1 cut(s) 52
AccII CGCG 1 cut(s) 189
AciI CCGC 5 cut(s) 128, 131, 204, 245, 631
AclI AACGTT 1 cut(s) 582
AclWI GGATC 4 cut(s) 2, 15, 305, 890
AcsI RAATTY 3 cut(s) 479, 598, 1101
AcuI CTGAAG 3 cut(s) 479, 732, 1221
AcyI GRCGYC 1 cut(s) 195
AdeI CACNNNGTG 1 cut(s) 110
AfaI GTAC 6 cut(s) 44, 303, 348, 794, 880, 932
AfiI CCNNNNNNNGG 2 cut(s) 133, 637
AflII CTTAAG 1 cut(s) 979
AgsI TTSAA 5 cut(s) 554, 590, 618, 698, 887
AhlI ACTAGT 1 cut(s) 266
AjiI CACGTC 1 cut(s) 628
AjnI CCWGG 4 cut(s) 69, 335, 652, 1114
AluBI AGCT 4 cut(s) 217, 754, 866, 1110
AluI AGCT 4 cut(s) 217, 754, 866, 1110
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 4 cut(s) 2, 15, 305, 890
AlwNI CAGNNNCTG 1 cut(s) 1199
AoxI GGCC 2 cut(s) 258, 994
ApeKI GCWGC 2 cut(s) 284, 533
ApoI RAATTY 3 cut(s) 479, 598, 1101
AspLEI GCGC 3 cut(s) 191, 652, 953
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 2 cut(s) 115, 1086
BamHI GGATCC 1 cut(s) 7
BbvI GCAGC 2 cut(s) 271, 545
BccI CCATC 2 cut(s) 54, 1076
BceAI ACGGC 3 cut(s) 79, 93, 429
BciT130I CCWGG 4 cut(s) 71, 337, 654, 1116
BciVI GTATCC 2 cut(s) 328, 493
BclI TGATCA 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 414
BcuI ACTAGT 1 cut(s) 266
BfaI CTAG 3 cut(s) 267, 876, 893
BfoI RGCGCY 1 cut(s) 954
BfrI CTTAAG 1 cut(s) 979
BfuI GTATCC 2 cut(s) 328, 493
BglII AGATCT 1 cut(s) 1216
BisI GCNGC 4 cut(s) 131, 204, 285, 534
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 4 cut(s) 132, 205, 286, 535
BmcAI AGTACT 2 cut(s) 303, 880
Bme1390I CCNGG 4 cut(s) 71, 337, 654, 1116
BmgBI CACGTC 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 2 cut(s) 9, 75
BmrFI CCNGG 4 cut(s) 71, 337, 654, 1116
BmsI GCATC 4 cut(s) 637, 1128, 1136, 1156
BpmI CTGGAG 3 cut(s) 53, 358, 414
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 239, 841
BsaHI GRCGYC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 504, 653, 922, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 637
Bse1I ACTGG 4 cut(s) 40, 431, 666, 1066
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 4 cut(s) 71, 337, 654, 1116
BseDI CCNNGG 4 cut(s) 504, 653, 922, 991
BseGI GGATG 5 cut(s) 253, 670, 1095, 1143, 1171
BseLI CCNNNNNNNGG 2 cut(s) 133, 637
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 193, 381
BseNI ACTGG 4 cut(s) 40, 431, 666, 1066
BseRI GAGGAG 3 cut(s) 574, 704, 858
BseXI GCAGC 2 cut(s) 271, 545
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 260, 996
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 2 cut(s) 133, 637
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 654
BsmI GAATGC 1 cut(s) 722
BsnI GGCC 2 cut(s) 260, 996
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
Bsp1720I GCTNAGC 1 cut(s) 375
BspACI CCGC 5 cut(s) 128, 131, 204, 245, 631
BspANI GGCC 2 cut(s) 260, 996
BspCNI CTCAG 2 cut(s) 192, 382
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 9, 75
BspPI GGATC 4 cut(s) 2, 15, 305, 890
BspTI CTTAAG 1 cut(s) 979
BsrDI GCAATG 1 cut(s) 852
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 4 cut(s) 40, 431, 666, 1066
BssECI CCNNGG 4 cut(s) 504, 653, 922, 991
BssMI GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 3 cut(s) 504, 922, 991
Bst2UI CCWGG 4 cut(s) 71, 337, 654, 1116
Bst4CI ACNGT 6 cut(s) 254, 455, 766, 930, 943, 1015
BstACI GRCGYC 1 cut(s) 195
BstAFI CTTAAG 1 cut(s) 979
BstAPI GCANNNNNTGC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 276, 722
BstDEI CTNAG 5 cut(s) 179, 213, 375, 390, 474
BstF5I GGATG 5 cut(s) 253, 670, 1095, 1143, 1171
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 954
BstHHI GCGC 3 cut(s) 191, 652, 953
BstKTI GATC 7 cut(s) 10, 313, 357, 670, 898, 1193, 1219
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
BstMWI GCNNNNNNNGC 8 cut(s) 24, 114, 203, 284, 831, 863, 872, 1116
BstNI CCWGG 4 cut(s) 71, 337, 654, 1116
BstSCI CCNGG 4 cut(s) 69, 335, 652, 1114
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 271, 545
BstX2I RGATCY 3 cut(s) 7, 895, 1216
BstXI CCANNNNNNTGG 2 cut(s) 269, 336
BstYI RGATCY 3 cut(s) 7, 895, 1216
BsuI GTATCC 2 cut(s) 328, 493
BsuRI GGCC 2 cut(s) 260, 996
BtrI CACGTC 1 cut(s) 628
BtsCI GGATG 5 cut(s) 253, 670, 1095, 1143, 1171
BtsIMutI CAGTG 1 cut(s) 1059
Cac8I GCNNGC 2 cut(s) 276, 722
CaiI CAGNNNCTG 1 cut(s) 1199
CfoI GCGC 3 cut(s) 191, 652, 953
Cfr13I GGNCC 1 cut(s) 259
CseI GACGC 2 cut(s) 203, 740
Csp6I GTAC 6 cut(s) 43, 302, 347, 793, 879, 931
CviAII CATG 2 cut(s) 644, 872
CviQI GTAC 6 cut(s) 43, 302, 347, 793, 879, 931
DdeI CTNAG 5 cut(s) 179, 213, 375, 390, 474
DpnI GATC 7 cut(s) 9, 312, 356, 669, 897, 1192, 1218
DpnII GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
DraI TTTAAA 2 cut(s) 525, 679
DraIII CACNNNGTG 1 cut(s) 110
Eco130I CCWWGG 3 cut(s) 504, 922, 991
Eco32I GATATC 1 cut(s) 817
Eco57I CTGAAG 3 cut(s) 479, 732, 1221
EcoRII CCWGG 4 cut(s) 69, 335, 652, 1114
EcoRV GATATC 1 cut(s) 817
EcoT14I CCWWGG 3 cut(s) 504, 922, 991
ErhI CCWWGG 3 cut(s) 504, 922, 991
FaeI CATG 2 cut(s) 647, 875
FalI AAGNNNNNCTT 4 cut(s) 782, 814, 1194, 1226
FaqI GGGAC 1 cut(s) 654
FatI CATG 2 cut(s) 643, 871
FauI CCCGC 1 cut(s) 135
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 131, 204, 285, 534
FokI GGATG 5 cut(s) 260, 677, 1102, 1150, 1178
Fsp4HI GCNGC 4 cut(s) 131, 204, 285, 534
FspBI CTAG 3 cut(s) 267, 876, 893
GlaI GCGC 3 cut(s) 190, 651, 952
GluI GCNGC 4 cut(s) 131, 204, 285, 534
GsuI CTGGAG 3 cut(s) 53, 358, 414
HaeII RGCGCY 1 cut(s) 954
HaeIII GGCC 2 cut(s) 260, 996
HapII CCGG 1 cut(s) 134
HgaI GACGC 2 cut(s) 203, 740
HhaI GCGC 3 cut(s) 191, 652, 953
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 2 cut(s) 647, 875
Hin6I GCGC 3 cut(s) 189, 650, 951
HinP1I GCGC 3 cut(s) 189, 650, 951
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 8 cut(s) 13, 31, 100, 146, 407, 427, 698, 841
HpaI GTTAAC 1 cut(s) 471
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 115, 1086
Hpy166II GTNNAC 4 cut(s) 53, 185, 347, 471
Hpy188I TCNGA 7 cut(s) 12, 78, 169, 391, 488, 947, 1195
Hpy188III TCNNGA 7 cut(s) 497, 518, 587, 820, 838, 893, 899
Hpy8I GTNNAC 4 cut(s) 53, 185, 347, 471
Hpy99I CGWCG 2 cut(s) 100, 197
HpyAV CCTTC 5 cut(s) 584, 631, 763, 836, 998
HpyCH4III ACNGT 6 cut(s) 254, 455, 766, 930, 943, 1015
HpyCH4IV ACGT 2 cut(s) 582, 627
HpyCH4V TGCA 5 cut(s) 404, 720, 857, 1071, 1141
HpyF10VI GCNNNNNNNGC 8 cut(s) 24, 114, 203, 284, 831, 863, 872, 1116
HpyF3I CTNAG 5 cut(s) 179, 213, 375, 390, 474
HpySE526I ACGT 2 cut(s) 582, 627
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 2 cut(s) 647, 875
HspAI GCGC 3 cut(s) 189, 650, 951
Ksp22I TGATCA 1 cut(s) 667
KspAI GTTAAC 1 cut(s) 471
Kzo9I GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
LmnI GCTCC 3 cut(s) 72, 79, 871
Lsp1109I GCAGC 2 cut(s) 271, 545
LweI GCATC 4 cut(s) 637, 1128, 1136, 1156
MaeI CTAG 3 cut(s) 267, 876, 893
MaeII ACGT 2 cut(s) 582, 627
MaeIII GTNAC 3 cut(s) 433, 971, 1092
MalI GATC 7 cut(s) 9, 312, 356, 669, 897, 1192, 1218
MboI GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
MboII GAAGA 8 cut(s) 155, 251, 364, 725, 785, 1091, 1122, 1156
MflI RGATCY 3 cut(s) 7, 895, 1216
MluCI AATT 7 cut(s) 35, 479, 598, 680, 985, 1101, 1231
MlyI GAGTC 4 cut(s) 22, 25, 416, 421
MmeI TCCRAC 2 cut(s) 101, 525
MslI CAYNNNNRTG 1 cut(s) 1164
MspA1I CMGCKG 1 cut(s) 633
MspCI CTTAAG 1 cut(s) 979
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 4 cut(s) 71, 337, 654, 1116
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 4 cut(s) 71, 337, 654, 1116
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 8 cut(s) 24, 114, 203, 284, 831, 863, 872, 1116
NdeII GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
NlaIII CATG 2 cut(s) 647, 875
NlaIV GGNNCC 2 cut(s) 9, 75
NmuCI GTSAC 1 cut(s) 1092
PctI GAATGC 1 cut(s) 722
PfeI GAWTC 4 cut(s) 100, 146, 698, 841
PfoI TCCNGGA 1 cut(s) 335
PkrI GCNGC 4 cut(s) 132, 205, 286, 535
PleI GAGTC 4 cut(s) 21, 25, 415, 421
PpsI GAGTC 4 cut(s) 21, 25, 415, 421
PsiI TTATAA 1 cut(s) 758
Psp1406I AACGTT 1 cut(s) 582
Psp6I CCWGG 4 cut(s) 69, 335, 652, 1114
PspGI CCWGG 4 cut(s) 69, 335, 652, 1114
PspN4I GGNNCC 2 cut(s) 9, 75
PspPI GGNCC 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 3 cut(s) 7, 895, 1216
RsaI GTAC 6 cut(s) 44, 303, 348, 794, 880, 932
RsaNI GTAC 6 cut(s) 43, 302, 347, 793, 879, 931
RseI CAYNNNNRTG 1 cut(s) 1164
SatI GCNGC 4 cut(s) 131, 204, 285, 534
Sau3AI GATC 7 cut(s) 7, 310, 354, 667, 895, 1190, 1216
Sau96I GGNCC 1 cut(s) 259
ScaI AGTACT 2 cut(s) 303, 880
SchI GAGTC 4 cut(s) 22, 25, 416, 421
ScrFI CCNGG 4 cut(s) 71, 337, 654, 1116
SfaNI GCATC 4 cut(s) 637, 1128, 1136, 1156
SmiMI CAYNNNNRTG 1 cut(s) 1164
SmlI CTYRAG 3 cut(s) 218, 820, 979
SmoI CTYRAG 3 cut(s) 218, 820, 979
SpeI ACTAGT 1 cut(s) 266
Sse9I AATT 7 cut(s) 35, 479, 598, 680, 985, 1101, 1231
SsiI CCGC 5 cut(s) 128, 131, 204, 245, 631
SspMI CTAG 3 cut(s) 267, 876, 893
StyD4I CCNGG 4 cut(s) 69, 335, 652, 1114
StyI CCWWGG 3 cut(s) 504, 922, 991
TaaI ACNGT 6 cut(s) 254, 455, 766, 930, 943, 1015
TaiI ACGT 2 cut(s) 585, 630
TaqI TCGA 1 cut(s) 98
TasI AATT 7 cut(s) 35, 479, 598, 680, 985, 1101, 1231
TatI WGTACW 4 cut(s) 301, 346, 878, 930
TauI GCSGC 2 cut(s) 133, 206
TfiI GAWTC 4 cut(s) 100, 146, 698, 841
TscAI CASTG 1 cut(s) 1066
TseFI GTSAC 1 cut(s) 1092
TseI GCWGC 2 cut(s) 284, 533
Tsp45I GTSAC 1 cut(s) 1092
TspDTI ATGAA 4 cut(s) 156, 472, 1029, 1094
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 1 cut(s) 1066
Vha464I CTTAAG 1 cut(s) 979
XapI RAATTY 3 cut(s) 479, 598, 1101
XbaI TCTAGA 1 cut(s) 892
XmiI GTMKAC 1 cut(s) 52
XspI CTAG 3 cut(s) 267, 876, 893
ZrmI AGTACT 2 cut(s) 303, 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.