Rroxscaffold_7G00195690

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017742
Physical Location & Seq
Forward (+)
38254319 .. 38254642
324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195690.1

Sequence Viewer

Length: 324 bp
ATGAAACGTTATTATCTTCAGCCTCAATCTCTTGGACCCATTCAAAGATTTCCAATTCTCTTCCTCATCGTTCTCCATATTCGAGAAAACTCAGCTTTCATGTCACCAGGATTGACTGGAAGGAGACCTCAGAAGGCAAAAGCCCATGTGTTCAAGGTTGATATTTCGGGGCCGAAGAAAGAAGAGGTCAAGGTTGAGATTGAAGATGACGGGGTGCTACGGATCAGCAGAGAAAGGAAGATAGAGGAGGACAAGAACGGCAAGTGGCATCGGATCGAGAGAAGCAGCGACAAGTTCTCGAGAAGGTTTGAATGCGAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

107

Amino Acids

12.64

Weight (kDa)

9.8

Isoelectric Point (pI)

86.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 45 - 104 1.2e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017742)

Species Orthologous Gene IDs
malus_domestica MD11G1087200.v1.1 MD11G1089400.v1.1
rosa_chinensis RchiOBHm_Chr6g0273051
rosa_laevigata RLG00000016766
rosa_multiflora Rmu_co8398661.1_g000001 Rmu_sc0000239.1_g000121
rosa_roxburghii Rroxscaffold_7G00195690
rosa_rugosa Rorug03G0091000 Rorug06G0072600
rosa_samantha Rh6AG188200 Rh6BG191600 Rh6DG182100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 7
AclWI GGATC 2 cut(s) 230, 281
AgsI TTSAA 4 cut(s) 44, 154, 203, 311
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 2 cut(s) 230, 281
Ama87I CYCGRG 1 cut(s) 298
AoxI GGCC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 35, 170
AsuHPI GGTGA 1 cut(s) 96
AvaI CYCGRG 1 cut(s) 298
AvaII GGWCC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 297
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 118
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 298
BmgT120I GGNCC 2 cut(s) 35, 170
BmiI GGNNCC 2 cut(s) 37, 171
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 108
BseMII CTCAG 2 cut(s) 105, 143
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 1 cut(s) 297
BshFI GGCC 1 cut(s) 172
BsiHKCI CYCGRG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 118
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 298
Bsp143I GATC 2 cut(s) 222, 273
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 2 cut(s) 104, 142
BspLI GGNNCC 2 cut(s) 37, 171
BspPI GGATC 2 cut(s) 230, 281
BspTNI GGTCTC 1 cut(s) 118
BsrI ACTGG 1 cut(s) 121
BssMI GATC 2 cut(s) 222, 273
Bst2UI CCWGG 1 cut(s) 108
Bst6I CTCTTC 2 cut(s) 65, 177
BstDEI CTNAG 2 cut(s) 91, 129
BstKTI GATC 2 cut(s) 225, 276
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 2 cut(s) 222, 273
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 172
Cfr13I GGNCC 2 cut(s) 35, 170
CviAII CATG 2 cut(s) 100, 146
CviJI RGCY 4 cut(s) 22, 95, 143, 172
CviKI_1 RGCY 4 cut(s) 22, 95, 143, 172
DdeI CTNAG 2 cut(s) 91, 129
DpnI GATC 2 cut(s) 224, 275
DpnII GATC 2 cut(s) 222, 273
Eam1104I CTCTTC 2 cut(s) 65, 177
EarI CTCTTC 2 cut(s) 65, 177
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 103, 149
FaiI YATR 3 cut(s) 78, 101, 147
FatI CATG 2 cut(s) 99, 145
Fnu4HI GCNGC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 286
GluI GCNGC 1 cut(s) 286
HaeIII GGCC 1 cut(s) 172
Hin1II CATG 2 cut(s) 103, 149
HphI GGTGA 1 cut(s) 96
Hpy188I TCNGA 2 cut(s) 132, 273
Hpy188III TCNNGA 4 cut(s) 83, 277, 298, 300
HpyAV CCTTC 3 cut(s) 114, 127, 297
HpyCH4IV ACGT 1 cut(s) 7
HpyF3I CTNAG 2 cut(s) 91, 129
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 2 cut(s) 103, 149
Kzo9I GATC 2 cut(s) 222, 273
LpnPI CCDG 3 cut(s) 93, 102, 120
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 1 cut(s) 277
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 102
MalI GATC 2 cut(s) 224, 275
MboI GATC 2 cut(s) 222, 273
MboII GAAGA 6 cut(s) 8, 52, 187, 194, 215, 250
MluCI AATT 1 cut(s) 54
MnlI CCTC 6 cut(s) 33, 74, 138, 178, 238, 241
MseI TTAA 1 cut(s) 322
MspR9I CCNGG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 1 cut(s) 108
NdeII GATC 2 cut(s) 222, 273
NlaIII CATG 2 cut(s) 103, 149
NlaIV GGNNCC 2 cut(s) 37, 171
NmuCI GTSAC 1 cut(s) 102
PaeR7I CTCGAG 1 cut(s) 298
PctI GAATGC 1 cut(s) 317
PkrI GCNGC 1 cut(s) 287
Psp1406I AACGTT 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 37, 171
PspPI GGNCC 2 cut(s) 35, 170
SaqAI TTAA 1 cut(s) 322
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 2 cut(s) 222, 273
Sau96I GGNCC 2 cut(s) 35, 170
ScrFI CCNGG 1 cut(s) 108
SetI ASST 7 cut(s) 10, 97, 130, 159, 189, 195, 308
SfaNI GCATC 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 298
SinI GGWCC 1 cut(s) 35
SlaI CTCGAG 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 106
TaiI ACGT 1 cut(s) 10
TaqI TCGA 3 cut(s) 82, 276, 299
TasI AATT 1 cut(s) 54
Tru1I TTAA 1 cut(s) 322
Tru9I TTAA 1 cut(s) 322
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 1 cut(s) 285
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 2 cut(s) 17, 88
TspGWI ACGGA 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.