Rorug06G0072600

kDa class I heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015734
Physical Location & Seq
Forward (+)
9676738 .. 9680002
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0072600.1

Sequence Viewer

Length: 786 bp
ATGAACGTCGCCCTTCTCCATCCCCTCTTTCTCACATTTTCTTGCTTATTCTTCCTTGCTTCCTCCTCCTCCTCCTCAGGCTACAGTCCCGGCGACACTCCAAAACCCGTTAGGAGGGAAGTATACGGCAATGGGAGAATCTTCGACATCACCCACAGGTACACCCCGGAGATTCCCGTGTATGGTTCAAAGCTTGGGATCGGACATTTCCGGTGGCTCCAACTGAGCATGAAGAATGGTTCACTTACCAACAACTCGGAGTTCAAGCTGCCTGTTCACACCGGAACCCATGTTGATGCCCCTGGCCATGTGTTTGATGATTACTATGATGCTGGGTTTGATGTAGATCAGCTTGACTTGGAGGTACTCAATGGCCCTGCCCTTGTTGTTGATGTTCCCAGGGATAGCAACATAACTGCTGAAGTGATGGAGTCCTTAAATATTCCCAAGGGTACTCGCCGAGTACTTTTCAGGACATTAAATACTGATAGGGGTCTTATGCATAAAAAGGAATTTGACTCAAGCTTTGTGGGATTCACGGAGGATGGAGCGAAATGGGTGGTTAAGAACACGGACATCAAACTTGTTGGAACTGATTACTTTTCCATTGCTACCTATGATGAAGCTATTCCGGCACATATTGCTTTTCTAAAGGGAAGGGAAATCATACCTGTGGAAGGCCTAAAGCTTGATGGCATAAAACCAGGAAATTATTCTGTGCATTGCTTACCTCTAAGGTTGCTTGGTGCTGAAGGAGCACCAACAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

261

Amino Acids

28.92

Weight (kDa)

6.3

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 48 - 203 1.7e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 512
AcuI CTGAAG 2 cut(s) 441, 771
AfaI GTAC 4 cut(s) 161, 366, 454, 465
AfiI CCNNNNNNNGG 3 cut(s) 114, 182, 677
AgsI TTSAA 2 cut(s) 189, 265
AjnI CCWGG 3 cut(s) 301, 398, 703
AluBI AGCT 6 cut(s) 193, 268, 352, 525, 626, 688
AluI AGCT 6 cut(s) 193, 268, 352, 525, 626, 688
Alw21I GWGCWC 1 cut(s) 760
AlwI GGATC 1 cut(s) 206
AoxI GGCC 3 cut(s) 304, 373, 679
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 1 cut(s) 512
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
Asp700I GAANNNNTTC 2 cut(s) 627, 712
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 2 cut(s) 90, 167
AsuHPI GGTGA 1 cut(s) 142
AxyI CCTNAGG 1 cut(s) 76
BalI TGGCCA 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 760
BbvI GCAGC 1 cut(s) 255
BccI CCATC 4 cut(s) 27, 421, 539, 686
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 3 cut(s) 303, 400, 705
BcnI CCSGG 2 cut(s) 90, 167
BfmI CTRYAG 1 cut(s) 82
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 5 cut(s) 90, 167, 303, 400, 705
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 218, 286
BmrFI CCNGG 5 cut(s) 90, 167, 303, 400, 705
BmsI GCATC 3 cut(s) 286, 319, 758
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 2 cut(s) 90, 167
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 5 cut(s) 165, 301, 398, 399, 447
BsaWI WCCGGW 2 cut(s) 210, 281
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 182, 677
Bse21I CCTNAGG 1 cut(s) 76
Bse3DI GCAATG 3 cut(s) 136, 606, 721
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 3 cut(s) 303, 400, 705
BseDI CCNNGG 5 cut(s) 165, 301, 398, 399, 447
BseGI GGATG 2 cut(s) 19, 550
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 114, 182, 677
BseMI GCAATG 3 cut(s) 136, 606, 721
BseMII CTCAG 2 cut(s) 90, 215
BseRI GAGGAG 4 cut(s) 55, 58, 61, 64
BseXI GCAGC 1 cut(s) 255
BseYI CCCAGC 1 cut(s) 332
BshFI GGCC 3 cut(s) 306, 375, 681
BsiHKAI GWGCWC 1 cut(s) 760
BsiSI CCGG 5 cut(s) 90, 167, 211, 282, 632
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 3 cut(s) 114, 182, 677
BsmFI GGGAC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 771
BsnI GGCC 3 cut(s) 306, 375, 681
Bsp1286I GDGCHC 1 cut(s) 760
Bsp143I GATC 2 cut(s) 198, 346
BspANI GGCC 3 cut(s) 306, 375, 681
BspCNI CTCAG 2 cut(s) 89, 216
BspLI GGNNCC 2 cut(s) 218, 286
BspPI GGATC 1 cut(s) 206
BsrDI GCAATG 3 cut(s) 136, 606, 721
BssECI CCNNGG 5 cut(s) 165, 301, 398, 399, 447
BssMI GATC 2 cut(s) 198, 346
BssNAI GTATAC 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 447
Bst1107I GTATAC 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 303, 400, 705
Bst4CI ACNGT 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 641
BstDEI CTNAG 3 cut(s) 76, 224, 734
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 2 cut(s) 19, 550
BstKTI GATC 2 cut(s) 201, 349
BstMBI GATC 2 cut(s) 198, 346
BstMWI GCNNNNNNNGC 3 cut(s) 632, 641, 755
BstNI CCWGG 3 cut(s) 303, 400, 705
BstSCI CCNGG 5 cut(s) 88, 165, 301, 398, 703
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 255
BstZ17I GTATAC 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 76
BsuRI GGCC 3 cut(s) 306, 375, 681
BtsCI GGATG 2 cut(s) 19, 550
Cfr13I GGNCC 1 cut(s) 374
Csp6I GTAC 4 cut(s) 160, 365, 453, 464
CspCI CAANNNNNGTGG 2 cut(s) 510, 545
CviAII CATG 3 cut(s) 229, 290, 308
CviQI GTAC 4 cut(s) 160, 365, 453, 464
DdeI CTNAG 3 cut(s) 76, 224, 734
DpnI GATC 2 cut(s) 200, 348
DpnII GATC 2 cut(s) 198, 346
EaeI YGGCCR 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 681
Eco57I CTGAAG 2 cut(s) 441, 771
Eco81I CCTNAGG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoRII CCWGG 3 cut(s) 301, 398, 703
EcoT14I CCWWGG 1 cut(s) 447
EcoT22I ATGCAT 2 cut(s) 504, 773
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 3 cut(s) 232, 293, 311
FaqI GGGAC 1 cut(s) 72
FatI CATG 3 cut(s) 228, 289, 307
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 6, 557
Fsp4HI GCNGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 269
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 3 cut(s) 306, 375, 681
HapII CCGG 5 cut(s) 90, 167, 211, 282, 632
Hin1II CATG 3 cut(s) 232, 293, 311
HindIII AAGCTT 3 cut(s) 191, 523, 686
HinfI GANTC 5 cut(s) 138, 172, 431, 518, 534
HpaII CCGG 5 cut(s) 90, 167, 211, 282, 632
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 4 cut(s) 124, 162, 242, 277
Hpy188I TCNGA 2 cut(s) 203, 259
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 4 cut(s) 124, 162, 242, 277
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 23, 651, 671, 746
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 3 cut(s) 502, 721, 771
HpyF10VI GCNNNNNNNGC 3 cut(s) 632, 641, 755
HpyF3I CTNAG 3 cut(s) 76, 224, 734
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 3 cut(s) 232, 293, 311
Kzo9I GATC 2 cut(s) 198, 346
LmnI GCTCC 3 cut(s) 222, 548, 755
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 3 cut(s) 286, 319, 758
MaeII ACGT 1 cut(s) 6
MalI GATC 2 cut(s) 200, 348
MboI GATC 2 cut(s) 198, 346
MboII GAAGA 3 cut(s) 43, 133, 244
MhlI GDGCHC 1 cut(s) 760
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 2 cut(s) 512, 709
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 2 cut(s) 440, 512
MmeI TCCRAC 2 cut(s) 244, 568
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 2 cut(s) 504, 773
MroXI GAANNNNTTC 2 cut(s) 627, 712
MscI TGGCCA 1 cut(s) 306
MseI TTAA 3 cut(s) 437, 479, 564
MslI CAYNNNNRTG 2 cut(s) 294, 671
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 5 cut(s) 90, 167, 211, 282, 632
MspR9I CCNGG 5 cut(s) 90, 167, 303, 400, 705
Mva1269I GAATGC 1 cut(s) 771
MvaI CCWGG 3 cut(s) 303, 400, 705
MwoI GCNNNNNNNGC 3 cut(s) 632, 641, 755
NciI CCSGG 2 cut(s) 90, 167
NdeII GATC 2 cut(s) 198, 346
NlaIII CATG 3 cut(s) 232, 293, 311
NlaIV GGNNCC 2 cut(s) 218, 286
NmeAIII GCCGAG 1 cut(s) 485
NsiI ATGCAT 2 cut(s) 504, 773
PasI CCCWGGG 1 cut(s) 399
PceI AGGCCT 1 cut(s) 681
PctI GAATGC 1 cut(s) 771
PdmI GAANNNNTTC 2 cut(s) 627, 712
PfeI GAWTC 3 cut(s) 138, 172, 534
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 2 cut(s) 439, 512
PpsI GAGTC 2 cut(s) 439, 512
Psp6I CCWGG 3 cut(s) 301, 398, 703
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 3 cut(s) 301, 398, 703
PspN4I GGNNCC 2 cut(s) 218, 286
PspPI GGNCC 1 cut(s) 374
RsaI GTAC 4 cut(s) 161, 366, 454, 465
RsaNI GTAC 4 cut(s) 160, 365, 453, 464
RseI CAYNNNNRTG 2 cut(s) 294, 671
SaqAI TTAA 3 cut(s) 437, 479, 564
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 2 cut(s) 198, 346
Sau96I GGNCC 1 cut(s) 374
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 2 cut(s) 440, 512
ScrFI CCNGG 5 cut(s) 90, 167, 303, 400, 705
SduI GDGCHC 1 cut(s) 760
SfaNI GCATC 3 cut(s) 286, 319, 758
SfcI CTRYAG 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 294, 671
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 2 cut(s) 512, 709
SseBI AGGCCT 1 cut(s) 681
SspI AATATT 1 cut(s) 442
StuI AGGCCT 1 cut(s) 681
StyD4I CCNGG 5 cut(s) 88, 165, 301, 398, 703
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 1 cut(s) 9
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 512, 709
TatI WGTACW 1 cut(s) 463
TfiI GAWTC 3 cut(s) 138, 172, 534
Tru1I TTAA 3 cut(s) 437, 479, 564
Tru9I TTAA 3 cut(s) 437, 479, 564
TseI GCWGC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 17, 245, 636
TspGWI ACGGA 2 cut(s) 554, 587
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 1 cut(s) 512
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 2 cut(s) 627, 712
ZrmI AGTACT 1 cut(s) 465
Zsp2I ATGCAT 2 cut(s) 504, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.