RLG00000014134

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015734
Physical Location & Seq
Forward (+)
47837498 .. 47840820
3323 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014134

Sequence Viewer

Length: 360 bp
ATGAACAAGCATGGAAGTAATGTAATCGAGAAAACCTTGAAAGAGGCTGGAGAAGAACATGCCAACATGATTATTAACGAGATGATTAACAGTCCTGAGTTTCTGAATGTTCTTCAAAATCCTTATGGGAATTATGCTGCTCAGTCTGCTCTTGCAGTATCCAAGATTGAAAATGAGTATGGACCCATGGAATACGAAATTGGTGCACCAGGTAAAGCCTACACTGACTGGGCAGCTCAAATGGTTGTGGGATTAAACACTAGTTTCCCCTGGATCCTGTGCAAACAAGATGATGCCCCTGATCCTGTTGTATTTGAGCAATTATGGTTCCAGGTAACCCCTCTGTTCTTCTCCATTTAA

Protein Analysis

120

Amino Acids

13.36

Weight (kDa)

4.41

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 1 - 29 2.8e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 268, 281, 296
AgsI TTSAA 3 cut(s) 40, 116, 170
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 3 cut(s) 208, 269, 330
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
Alw21I GWGCWC 1 cut(s) 208
Alw44I GTGCAC 1 cut(s) 204
AlwI GGATC 3 cut(s) 268, 281, 296
ApaLI GTGCAC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 137, 233
AspS9I GGNCC 1 cut(s) 182
AvaII GGWCC 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 208
BamHI GGATCC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 2 cut(s) 124, 245
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 3 cut(s) 210, 271, 332
BciVI GTATCC 1 cut(s) 169
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 1 cut(s) 261
BfuI GTATCC 1 cut(s) 169
BisI GCNGC 2 cut(s) 138, 234
BlsI GCNGC 2 cut(s) 139, 235
Bme1390I CCNGG 3 cut(s) 210, 271, 332
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 3 cut(s) 184, 275, 329
BmrFI CCNGG 3 cut(s) 210, 271, 332
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 186, 269
Bse1I ACTGG 1 cut(s) 233
BseBI CCWGG 3 cut(s) 210, 271, 332
BseDI CCNNGG 2 cut(s) 186, 269
BseMII CTCAG 2 cut(s) 87, 155
BseNI ACTGG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 2 cut(s) 124, 245
BsiHKAI GWGCWC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 273, 301
Bsp19I CCATGG 1 cut(s) 186
BspCNI CTCAG 2 cut(s) 88, 154
BspLI GGNNCC 3 cut(s) 184, 275, 329
BspPI GGATC 3 cut(s) 268, 281, 296
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 186, 269
BssMI GATC 2 cut(s) 273, 301
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 210, 271, 332
Bst4CI ACNGT 1 cut(s) 92
BstDEI CTNAG 2 cut(s) 96, 141
BstDSI CCRYGG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 334
BstKTI GATC 2 cut(s) 276, 304
BstMBI GATC 2 cut(s) 273, 301
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 3 cut(s) 210, 271, 332
BstNSI RCATGY 1 cut(s) 62
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 208, 269, 330
BstSLI GKGCMC 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 124, 245
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuI GTATCC 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 182
CsiI ACCWGGT 1 cut(s) 208
CviAII CATG 4 cut(s) 11, 59, 67, 187
CviJI RGCY 3 cut(s) 47, 218, 236
CviKI_1 RGCY 3 cut(s) 47, 218, 236
DdeI CTNAG 2 cut(s) 96, 141
DpnI GATC 2 cut(s) 275, 303
DpnII GATC 2 cut(s) 273, 301
Eco130I CCWWGG 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 334
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 208, 269, 330
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 14, 62, 70, 190
FaiI YATR 8 cut(s) 12, 60, 68, 126, 135, 180, 188, 325
FatI CATG 4 cut(s) 10, 58, 66, 186
Fnu4HI GCNGC 2 cut(s) 138, 234
Fsp4HI GCNGC 2 cut(s) 138, 234
FspBI CTAG 1 cut(s) 261
GluI GCNGC 2 cut(s) 138, 234
GsuI CTGGAG 1 cut(s) 69
Hin1II CATG 4 cut(s) 14, 62, 70, 190
Hpy166II GTNNAC 1 cut(s) 206
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 2 cut(s) 28, 95
Hpy8I GTNNAC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4V TGCA 3 cut(s) 155, 206, 282
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 2 cut(s) 96, 141
Hsp92II CATG 4 cut(s) 14, 62, 70, 190
Kzo9I GATC 2 cut(s) 273, 301
Lsp1109I GCAGC 2 cut(s) 124, 245
LweI GCATC 1 cut(s) 283
MabI ACCWGGT 1 cut(s) 208
MaeI CTAG 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 275, 303
MboI GATC 2 cut(s) 273, 301
MboII GAAGA 3 cut(s) 65, 104, 340
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 3 cut(s) 130, 198, 320
MnlI CCTC 2 cut(s) 37, 351
MseI TTAA 4 cut(s) 75, 87, 254, 358
MspR9I CCNGG 3 cut(s) 210, 271, 332
MvaI CCWGG 3 cut(s) 210, 271, 332
MwoI GCNNNNNNNGC 1 cut(s) 146
NcoI CCATGG 1 cut(s) 186
NdeII GATC 2 cut(s) 273, 301
NlaIII CATG 4 cut(s) 14, 62, 70, 190
NlaIV GGNNCC 3 cut(s) 184, 275, 329
NspI RCATGY 1 cut(s) 62
PkrI GCNGC 2 cut(s) 139, 235
Psp6I CCWGG 3 cut(s) 208, 269, 330
PspEI GGTNACC 1 cut(s) 334
PspGI CCWGG 3 cut(s) 208, 269, 330
PspN4I GGNNCC 3 cut(s) 184, 275, 329
PspPI GGNCC 1 cut(s) 182
PsuI RGATCY 1 cut(s) 273
SaqAI TTAA 4 cut(s) 75, 87, 254, 358
SatI GCNGC 2 cut(s) 138, 234
Sau3AI GATC 2 cut(s) 273, 301
Sau96I GGNCC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 210, 271, 332
SduI GDGCHC 1 cut(s) 208
SetI ASST 4 cut(s) 38, 214, 238, 336
SexAI ACCWGGT 1 cut(s) 208
SfaNI GCATC 1 cut(s) 283
SinI GGWCC 1 cut(s) 182
SpeI ACTAGT 1 cut(s) 260
Sse9I AATT 3 cut(s) 130, 198, 320
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 208, 269, 330
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 27
TasI AATT 3 cut(s) 130, 198, 320
Tru1I TTAA 4 cut(s) 75, 87, 254, 358
Tru9I TTAA 4 cut(s) 75, 87, 254, 358
TscAI CASTG 1 cut(s) 229
TseI GCWGC 2 cut(s) 137, 233
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 229
VneI GTGCAC 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 182
XceI RCATGY 1 cut(s) 62
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.