RLG00000013644

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015734
Physical Location & Seq
Reverse (-)
34900022 .. 34902369
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013644

Sequence Viewer

Length: 498 bp
ATGGTTGTGTCTTTGGATACAGGGGTGCCCTGGGTCATGTGCCAGCAAGCAGATGCTCCTTCTTCAGTGATCAACACTTGCAATGGATTTTACTGTGATCAATTCACCCCAATTTCTAATAGCACATCCAAAATGTGGACCGAGAACTGGAGTGGATGGATACAAATCTCCATGGAATATGGAGGAAAGTTCAGCCTTTTGCTTACCATGGTGGAACCAACTTTGGGCAGTCTAGTGGAGGACCTTTTATTGCTACAAGTTATGTCTGATGCTCCACTTGATGAGTATGGACTTCTTAGCATTGATGTCAAAGATGGATATAAAGATGATCTTCACGTGCAATCTGTTGGTCATGCTCTTCATGTTTTTGTCAATGGAAAGCTTGCAGGGAGTGGAACAGGCAAAAGTGGCAATGCCAAGGTTTCTGTGGAGATCCCTGTCGAATTTGCATCTGGGAAGAACACAATTGTTCTTCTGAGTCTGACTGTTGGGCTTTAG

Protein Analysis

166

Amino Acids

17.72

Weight (kDa)

4.49

Isoelectric Point (pI)

20.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 1 - 55 1.7e-06 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 135
AclWI GGATC 1 cut(s) 427
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 48
AcvI CACGTG 1 cut(s) 337
AfiI CCNNNNNNNGG 3 cut(s) 135, 147, 224
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
AlwI GGATC 1 cut(s) 427
ApoI RAATTY 1 cut(s) 443
AspS9I GGNCC 2 cut(s) 138, 241
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 2 cut(s) 138, 241
BaeGI GKGCMC 1 cut(s) 30
BaeI ACNNNNGTAYC 2 cut(s) 9, 42
BanI GGYRCC 1 cut(s) 25
BbrPI CACGTG 1 cut(s) 337
BccI CCATC 2 cut(s) 150, 308
BciT130I CCWGG 1 cut(s) 31
BciVI GTATCC 2 cut(s) 10, 153
BclI TGATCA 2 cut(s) 69, 97
BfaI CTAG 1 cut(s) 233
BfuI GTATCC 2 cut(s) 10, 153
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 2 cut(s) 138, 241
BmgT120I GGNCC 2 cut(s) 138, 241
BmiI GGNNCC 2 cut(s) 27, 216
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 3 cut(s) 43, 259, 458
BpmI CTGGAG 1 cut(s) 169
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 164
BsaJI CCNNGG 5 cut(s) 29, 30, 171, 207, 417
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 147, 224
Bse1I ACTGG 1 cut(s) 152
Bse3DI GCAATG 2 cut(s) 88, 418
Bse8I GATNNNNATC 1 cut(s) 164
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 5 cut(s) 29, 30, 171, 207, 417
BseGI GGATG 2 cut(s) 125, 161
BseJI GATNNNNATC 1 cut(s) 164
BseLI CCNNNNNNNGG 3 cut(s) 135, 147, 224
BseMI GCAATG 2 cut(s) 88, 418
BseMII CTCAG 1 cut(s) 467
BseNI ACTGG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 25
BslI CCNNNNNNNGG 3 cut(s) 135, 147, 224
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 4 cut(s) 69, 97, 328, 432
Bsp19I CCATGG 2 cut(s) 171, 207
BspCNI CTCAG 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 27, 216
BspPI GGATC 1 cut(s) 427
BspQI GCTCTTC 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 25
BsrDI GCAATG 2 cut(s) 88, 418
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 5 cut(s) 29, 30, 171, 207, 417
BssMI GATC 4 cut(s) 69, 97, 328, 432
BssT1I CCWWGG 3 cut(s) 171, 207, 417
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 2 cut(s) 95, 487
Bst6I CTCTTC 1 cut(s) 363
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 3 cut(s) 44, 48, 384
BstDEI CTNAG 2 cut(s) 296, 476
BstDSI CCRYGG 2 cut(s) 171, 207
BstF5I GGATG 2 cut(s) 125, 161
BstKTI GATC 4 cut(s) 72, 100, 331, 435
BstMBI GATC 4 cut(s) 69, 97, 328, 432
BstMWI GCNNNNNNNGC 1 cut(s) 408
BstNI CCWGG 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
BsuI GTATCC 2 cut(s) 10, 153
BtgI CCRYGG 2 cut(s) 171, 207
BtsCI GGATG 2 cut(s) 125, 161
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 3 cut(s) 44, 48, 384
Cfr13I GGNCC 2 cut(s) 138, 241
CviAII CATG 5 cut(s) 37, 172, 208, 353, 362
CviJI RGCY 3 cut(s) 195, 382, 493
CviKI_1 RGCY 3 cut(s) 195, 382, 493
DdeI CTNAG 2 cut(s) 296, 476
DpnI GATC 4 cut(s) 71, 99, 330, 434
DpnII GATC 4 cut(s) 69, 97, 328, 432
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Eco130I CCWWGG 3 cut(s) 171, 207, 417
Eco47I GGWCC 2 cut(s) 138, 241
Eco57I CTGAAG 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 29
EcoT14I CCWWGG 3 cut(s) 171, 207, 417
ErhI CCWWGG 3 cut(s) 171, 207, 417
FaeI CATG 5 cut(s) 40, 175, 211, 356, 365
FaiI YATR 9 cut(s) 38, 173, 180, 209, 263, 288, 321, 354, 363
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 5 cut(s) 36, 171, 207, 352, 361
FbaI TGATCA 2 cut(s) 69, 97
FokI GGATG 2 cut(s) 112, 168
FspBI CTAG 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 169
Hin1II CATG 5 cut(s) 40, 175, 211, 356, 365
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 478
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 3 cut(s) 268, 477, 483
Hpy8I GTNNAC 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 2 cut(s) 95, 487
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 4 cut(s) 81, 340, 386, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 408
HpyF3I CTNAG 2 cut(s) 296, 476
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 5 cut(s) 40, 175, 211, 356, 365
Ksp22I TGATCA 2 cut(s) 69, 97
Kzo9I GATC 4 cut(s) 69, 97, 328, 432
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 2 cut(s) 61, 277
LpnPI CCDG 9 cut(s) 6, 16, 43, 56, 133, 372, 384, 438, 450
LweI GCATC 3 cut(s) 43, 259, 458
MaeI CTAG 1 cut(s) 233
MaeII ACGT 1 cut(s) 336
MalI GATC 4 cut(s) 71, 99, 330, 434
MboI GATC 4 cut(s) 69, 97, 328, 432
MboII GAAGA 5 cut(s) 54, 323, 350, 464, 469
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 4 cut(s) 101, 111, 443, 465
MlyI GAGTC 1 cut(s) 487
MnlI CCTC 2 cut(s) 176, 232
MspR9I CCNGG 1 cut(s) 31
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 31
MwoI GCNNNNNNNGC 1 cut(s) 408
NcoI CCATGG 2 cut(s) 171, 207
NdeII GATC 4 cut(s) 69, 97, 328, 432
NlaIII CATG 5 cut(s) 40, 175, 211, 356, 365
NlaIV GGNNCC 2 cut(s) 27, 216
PasI CCCWGGG 1 cut(s) 30
PciSI GCTCTTC 1 cut(s) 363
PflMI CCANNNNNTGG 1 cut(s) 135
PleI GAGTC 1 cut(s) 486
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 1 cut(s) 486
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 29
PspCI CACGTG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 2 cut(s) 27, 216
PspPI GGNCC 2 cut(s) 138, 241
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 1 cut(s) 432
SapI GCTCTTC 1 cut(s) 363
Sau3AI GATC 4 cut(s) 69, 97, 328, 432
Sau96I GGNCC 2 cut(s) 138, 241
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 1 cut(s) 30
SetI ASST 4 cut(s) 246, 339, 384, 423
SfaNI GCATC 3 cut(s) 43, 259, 458
SinI GGWCC 2 cut(s) 138, 241
Sse9I AATT 4 cut(s) 101, 111, 443, 465
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 29
StyI CCWWGG 3 cut(s) 171, 207, 417
TaaI ACNGT 2 cut(s) 95, 487
TaiI ACGT 1 cut(s) 339
TaqI TCGA 1 cut(s) 441
TaqII GACCGA 1 cut(s) 155
TasI AATT 4 cut(s) 101, 111, 443, 465
TscAI CASTG 1 cut(s) 72
TspDTI ATGAA 1 cut(s) 350
TspRI CASTG 1 cut(s) 72
Van91I CCANNNNNTGG 1 cut(s) 135
VpaK11BI GGWCC 2 cut(s) 138, 241
XapI RAATTY 1 cut(s) 443
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.