Rorug06G0072800

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015734
Physical Location & Seq
Forward (+)
9683028 .. 9683651
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0072800.1

Sequence Viewer

Length: 624 bp
ATGAAACGCGACTTCACATGGGTCCAATCCATCGCCATCCTCTTTGGAAAACACCAACTCTTCATCGGCGCCCAAAAAACTGCTACATGGGATGACTCCGTTGACCGCCTTGCCCTCTCCTTCGACGGCGTGCCAATCACCCTCCGGGAATCCGAAGGCGCTAGGTGGCAATCCGAGAGCTTCCCACATGTCTCATTCGTAAGGGTGGGTGACACCAACAATGTGATGGTTGAAGTTGAAGGCAAGTTTAGGATCACAGCCAAGGTTGTGCCTATAACTGAAGAAGACTCTAGAGTTCACAACTATGGTGTGACAAAAGATGATGCGTTGGCTCATCTTGATCTTGGGTTTAAGTTCTTCTCTTTGAGCAACCGAGTTAATGGTGTGTTGGGTCAGACTTACAGACCCGATTACGTGAGCCGGGTCAAGATTGGAGCCAACATGCCTGTGATGAGTGGTGACAGGGAGTTCCAAACTTCAGGACTTTTCGCTGCGGATTGCTCCATTGCTAGGTTTAATGGTGCTAGCGACTCTGAGAATGAAGGGTCTGTTGAGGGTTTGGAGTTTCCAAGCTTGAACTGTGCTAGTGGGATTGATGGTCGAGGAGTTGTGTGCAAGAGATAG

Protein Analysis

207

Amino Acids

22.82

Weight (kDa)

5.6

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Root_cap PF06830 116 - 172 1.4e-29 Root cap
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccII CGCG 1 cut(s) 9
AciI CCGC 2 cut(s) 106, 494
AclWI GGATC 1 cut(s) 260
AcuI CTGAAG 2 cut(s) 300, 462
AcyI GRCGYC 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 510
AflIII ACRYGT 1 cut(s) 187
AgsI TTSAA 3 cut(s) 233, 239, 577
AluBI AGCT 2 cut(s) 180, 573
AluI AGCT 2 cut(s) 180, 573
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 491
AspLEI GCGC 2 cut(s) 71, 161
AspS9I GGNCC 1 cut(s) 22
AsuC2I CCSGG 2 cut(s) 146, 422
AsuHPI GGTGA 3 cut(s) 130, 221, 470
AsuNHI GCTAGC 1 cut(s) 524
AvaII GGWCC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 478
BccI CCATC 4 cut(s) 38, 44, 220, 590
BceAI ACGGC 1 cut(s) 142
BcnI CCSGG 2 cut(s) 146, 422
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 5 cut(s) 162, 291, 510, 525, 585
BfoI RGCGCY 2 cut(s) 72, 162
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 2 cut(s) 146, 422
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 3 cut(s) 23, 70, 436
BmrFI CCNGG 2 cut(s) 146, 422
BmsI GCATC 1 cut(s) 313
BmtI GCTAGC 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 291
BpuMI CCSGG 2 cut(s) 146, 422
BsaAI YACGTR 1 cut(s) 415
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 36, 97
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 618
BseXI GCAGC 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 9
BshNI GGYRCC 1 cut(s) 68
BsiSI CCGG 2 cut(s) 145, 421
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 1 cut(s) 196
Bsp143I GATC 2 cut(s) 252, 340
BspACI CCGC 2 cut(s) 106, 494
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 23, 70, 436
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 504
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 2 cut(s) 252, 340
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 581
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 131, 526
BstDEI CTNAG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 36, 97
BstFNI CGCG 1 cut(s) 9
BstH2I RGCGCY 2 cut(s) 72, 162
BstHHI GCGC 2 cut(s) 71, 161
BstKTI GATC 2 cut(s) 255, 343
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 2 cut(s) 252, 340
BstNSI RCATGY 2 cut(s) 191, 445
BstSCI CCNGG 2 cut(s) 144, 420
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 2 cut(s) 36, 97
Cac8I GCNNGC 2 cut(s) 131, 526
CfoI GCGC 2 cut(s) 71, 161
Cfr13I GGNCC 1 cut(s) 22
CviAII CATG 4 cut(s) 18, 87, 188, 442
CviJI RGCY 6 cut(s) 180, 260, 332, 420, 437, 573
CviKI_1 RGCY 6 cut(s) 180, 260, 332, 420, 437, 573
DdeI CTNAG 1 cut(s) 534
DinI GGCGCC 1 cut(s) 70
DpnI GATC 2 cut(s) 254, 342
DpnII GATC 2 cut(s) 252, 340
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 300, 462
EcoT14I CCWWGG 1 cut(s) 261
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 4 cut(s) 21, 90, 191, 445
FaiI YATR 6 cut(s) 19, 88, 189, 275, 306, 443
FatI CATG 4 cut(s) 17, 86, 187, 441
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 2 cut(s) 23, 104
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 5 cut(s) 162, 291, 510, 525, 585
GlaI GCGC 2 cut(s) 70, 160
GluI GCNGC 1 cut(s) 492
HaeII RGCGCY 2 cut(s) 72, 162
HapII CCGG 2 cut(s) 145, 421
HhaI GCGC 2 cut(s) 71, 161
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 4 cut(s) 21, 90, 191, 445
Hin6I GCGC 2 cut(s) 69, 159
HinP1I GCGC 2 cut(s) 69, 159
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 571
HinfI GANTC 4 cut(s) 95, 149, 287, 530
HpaII CCGG 2 cut(s) 145, 421
HphI GGTGA 3 cut(s) 130, 221, 470
Hpy166II GTNNAC 2 cut(s) 103, 298
Hpy188I TCNGA 4 cut(s) 154, 175, 396, 535
Hpy188III TCNNGA 4 cut(s) 291, 338, 427, 480
Hpy8I GTNNAC 2 cut(s) 103, 298
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 4 cut(s) 130, 149, 233, 536
HpyCH4III ACNGT 1 cut(s) 581
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 1 cut(s) 615
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 4 cut(s) 21, 90, 191, 445
HspAI GCGC 2 cut(s) 69, 159
KasI GGCGCC 1 cut(s) 68
Kzo9I GATC 2 cut(s) 252, 340
LmnI GCTCC 2 cut(s) 434, 506
LpnPI CCDG 5 cut(s) 158, 434, 448, 459, 465
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 313
MaeI CTAG 5 cut(s) 162, 291, 510, 525, 585
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 3 cut(s) 209, 310, 458
MalI GATC 2 cut(s) 254, 342
MboI GATC 2 cut(s) 252, 340
MboII GAAGA 4 cut(s) 52, 293, 296, 349
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 3 cut(s) 89, 281, 524
MnlI CCTC 5 cut(s) 50, 125, 152, 547, 596
MseI TTAA 3 cut(s) 351, 378, 516
MslI CAYNNNNRTG 2 cut(s) 303, 446
MspI CCGG 2 cut(s) 145, 421
MspR9I CCNGG 2 cut(s) 146, 422
MvnI CGCG 1 cut(s) 9
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 2 cut(s) 146, 422
NdeII GATC 2 cut(s) 252, 340
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 4 cut(s) 21, 90, 191, 445
NlaIV GGNNCC 3 cut(s) 23, 70, 436
NmuCI GTSAC 3 cut(s) 209, 310, 458
NspI RCATGY 2 cut(s) 191, 445
PciI ACATGT 1 cut(s) 187
PfeI GAWTC 1 cut(s) 149
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 3 cut(s) 89, 281, 524
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 3 cut(s) 89, 281, 524
Ppu21I YACGTR 1 cut(s) 415
PscI ACATGT 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 23, 70, 436
PspPI GGNCC 1 cut(s) 22
RseI CAYNNNNRTG 2 cut(s) 303, 446
SaqAI TTAA 3 cut(s) 351, 378, 516
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 2 cut(s) 252, 340
Sau96I GGNCC 1 cut(s) 22
SchI GAGTC 3 cut(s) 89, 281, 524
ScrFI CCNGG 2 cut(s) 146, 422
SetI ASST 6 cut(s) 167, 182, 267, 417, 515, 575
SfaNI GCATC 1 cut(s) 313
SfoI GGCGCC 1 cut(s) 70
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 303, 446
SsiI CCGC 2 cut(s) 106, 494
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 5 cut(s) 162, 291, 510, 525, 585
StyD4I CCNGG 2 cut(s) 144, 420
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 581
TaiI ACGT 1 cut(s) 417
TaqI TCGA 2 cut(s) 123, 601
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 3 cut(s) 351, 378, 516
Tru9I TTAA 3 cut(s) 351, 378, 516
TseFI GTSAC 3 cut(s) 209, 310, 458
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 3 cut(s) 209, 310, 458
TspDTI ATGAA 3 cut(s) 17, 52, 555
TspGWI ACGGA 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 22
XbaI TCTAGA 1 cut(s) 290
XceI RCATGY 2 cut(s) 191, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XspI CTAG 5 cut(s) 162, 291, 510, 525, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.