Rorug03G0091000

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017742
Physical Location & Seq
Forward (+)
7092603 .. 7095959
3357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0091000.1

Sequence Viewer

Length: 1188 bp
ATGGTGAAGCGCAAAGCGAGGAGGCTCAGAATAAATACAAATTACAAGTTTAGTGGGGCAGAGACAGAGAGAAAGGGAGAGCGCAGAGCCACAATGATGCCGAGAGTGGCCATGTCATGGCGCAATGGACTCAGAATGAGAATCCCATGGAGAACCAGAGCCCTCAACACCTTAAACGACGACGTTCACTCCTCCCGCCCAATTATCGTGTCTCCTCCTCTTGTCTCTCTAGACATTCCTGACGATTGGACTCCTTCTCCACCCTCTTCACATTTTGATTTTCCTATCAATTGCTCCCATGAGCAGACTACTGCTGTTATTGATGGGAAGTCTATTGCGGACGATATTAGGTCGAGAATAGGGGGTGAGGTAAGGAGGATGAAAGAGAACATTGGAATGGTGCCTGGGTTGGGTGTAATTATGGTTGGTCAGAGAAAGGACTCTGAGACTTATGTCAGGAACAAGATGATGGCTTGTGAAGAAGTTGGAATTAAATCAAATGTGGCTCACCTGCCTGAGCATTGTACAAAAGATCAGCTTCTTAAAGCTTTGTCAAAGTTTGATGTGGATCCCTCTGTTCATGGTATTCTCGTGCAACTTCCTCTACCACAACATTTGGATGAGAGAAAAATTATGGATGTGCTGAGTCCGGAGAAAGATGTGGATGGCTTCCATCCAATTAATATGGGAAATCTTGCCATGAGAGGAAGGGAGCCTATGTTTATTCCATGCACTCCAAAGGGTTGCATTGAGTTGTTGCTCAGGTCTGGCGTGGAAATAGTTGGGAAGAAAGCTGCTGTCATTGGCAGAAGTAATATTGTAGGATTGCCCACATCGTTGCTTTTGCAGAGGCACCATGCAACAGTCAGCATTGTACATGCATTCACAAAGAACCCAGAACATATCACCTGTGAAGCTGACATTGTGGTCACAGCTGCAGGAGTGCCTAATCTTGTCCGTGGAAATTGGCTAAAACCGGGAGCAGTTGTCATCGACGTTGGGACCAATCCAGTTGAGGACCCTAGCTGTGAGCGAGGTTATCGCCTCATAGGAGATGTTTGCTATGAAGAAGCAGTGCGGGTGGTGTCAGCCATTACCCCAGTCCCTGGAGGAGTTGGACCCATGACAATTGCTATGCTTCTATCAAATACTCTTGATTCAGCAAAGCGAGCCTACGCCTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

395

Amino Acids

43.56

Weight (kDa)

9.07

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THF_DHG_CYH PF00763 107 - 222 2.7e-39 Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C PF02882 243 - 391 1.8e-56 Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017742)

Species Orthologous Gene IDs
malus_domestica MD11G1087200.v1.1 MD11G1089400.v1.1
rosa_chinensis RchiOBHm_Chr6g0273051
rosa_laevigata RLG00000016766
rosa_multiflora Rmu_co8398661.1_g000001 Rmu_sc0000239.1_g000121
rosa_roxburghii Rroxscaffold_7G00195690
rosa_rugosa Rorug03G0091000 Rorug06G0072600
rosa_samantha Rh6AG188200 Rh6BG191600 Rh6DG182100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 519
AasI GACNNNNNNGTC 1 cut(s) 926
Acc36I ACCTGC 1 cut(s) 519
AccB1I GGYRCC 2 cut(s) 400, 852
AccB7I CCANNNNNTGG 2 cut(s) 117, 1106
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 3 cut(s) 196, 338, 1078
AclWI GGATC 2 cut(s) 563, 576
AcoI YGGCCR 1 cut(s) 108
AfaI GTAC 2 cut(s) 526, 876
AfiI CCNNNNNNNGG 3 cut(s) 117, 410, 1106
AjnI CCWGG 2 cut(s) 403, 1105
AluBI AGCT 6 cut(s) 538, 548, 794, 917, 935, 1026
AluI AGCT 6 cut(s) 538, 548, 794, 917, 935, 1026
Alw26I GTCTC 4 cut(s) 56, 216, 229, 440
AlwI GGATC 2 cut(s) 563, 576
AlwNI CAGNNNCTG 1 cut(s) 1106
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 2 cut(s) 794, 935
AseI ATTAAT 1 cut(s) 681
AspLEI GCGC 3 cut(s) 12, 84, 123
AspS9I GGNCC 3 cut(s) 1002, 1018, 1118
AsuC2I CCSGG 1 cut(s) 978
AsuHPI GGTGA 4 cut(s) 16, 377, 500, 898
AvaII GGWCC 3 cut(s) 1002, 1018, 1118
BalI TGGCCA 1 cut(s) 110
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 2 cut(s) 400, 852
BanII GRGCYC 1 cut(s) 163
BauI CACGAG 1 cut(s) 590
BbvI GCAGC 2 cut(s) 781, 922
BccI CCATC 4 cut(s) 317, 463, 659, 681
BciT130I CCWGG 2 cut(s) 405, 1107
BcnI CCSGG 1 cut(s) 978
BcoDI GTCTC 4 cut(s) 56, 216, 229, 440
BfaI CTAG 2 cut(s) 230, 1023
BfmI CTRYAG 1 cut(s) 936
BfuAI ACCTGC 1 cut(s) 519
BisI GCNGC 2 cut(s) 795, 936
BlsI GCNGC 2 cut(s) 796, 937
Bme1390I CCNGG 3 cut(s) 405, 978, 1107
Bme18I GGWCC 3 cut(s) 1002, 1018, 1118
BmgT120I GGNCC 3 cut(s) 1002, 1018, 1118
BmiI GGNNCC 7 cut(s) 402, 570, 714, 854, 1003, 1020, 1120
BmrFI CCNGG 3 cut(s) 405, 978, 1107
BmrI ACTGGG 1 cut(s) 1094
BmsI GCATC 1 cut(s) 87
BmuI ACTGGG 1 cut(s) 1094
BoxI GACNNNNGTC 1 cut(s) 452
BpmI CTGGAG 1 cut(s) 1128
Bpu10I CCTNAGC 2 cut(s) 516, 761
BpuMI CCSGG 1 cut(s) 978
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 4 cut(s) 146, 404, 958, 1105
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 8 cut(s) 173, 203, 241, 271, 704, 734, 972, 1002
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 410, 1106
Bse1I ACTGG 2 cut(s) 1010, 1100
Bse3DI GCAATG 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 567
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 2 cut(s) 405, 1107
BseDI CCNNGG 4 cut(s) 146, 404, 958, 1105
BseGI GGATG 5 cut(s) 384, 625, 643, 670, 673
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 3 cut(s) 117, 410, 1106
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 6 cut(s) 40, 145, 435, 507, 635, 775
BseNI ACTGG 2 cut(s) 1010, 1100
BseRI GAGGAG 5 cut(s) 34, 181, 204, 207, 1125
BseXI GCAGC 2 cut(s) 781, 922
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 2 cut(s) 400, 852
BsiSI CCGG 2 cut(s) 650, 977
BslFI GGGAC 2 cut(s) 1015, 1088
BslI CCNNNNNNNGG 3 cut(s) 117, 410, 1106
BsmAI GTCTC 4 cut(s) 56, 216, 229, 440
BsmFI GGGAC 2 cut(s) 1015, 1088
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 649
Bsp1407I TGTACA 2 cut(s) 524, 874
Bsp143I GATC 2 cut(s) 532, 568
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 3 cut(s) 196, 338, 1078
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 6 cut(s) 39, 144, 436, 508, 636, 774
BspEI TCCGGA 1 cut(s) 649
BspLI GGNNCC 7 cut(s) 402, 570, 714, 854, 1003, 1020, 1120
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 519
BspPI GGATC 2 cut(s) 563, 576
BspT107I GGYRCC 2 cut(s) 400, 852
BsrDI GCAATG 1 cut(s) 130
BsrGI TGTACA 2 cut(s) 524, 874
BsrI ACTGG 2 cut(s) 1010, 1100
BssECI CCNNGG 4 cut(s) 146, 404, 958, 1105
BssMI GATC 2 cut(s) 532, 568
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 590
Bst2UI CCWGG 2 cut(s) 405, 1107
Bst4CI ACNGT 1 cut(s) 865
Bst6I CTCTTC 1 cut(s) 271
BstAUI TGTACA 2 cut(s) 524, 874
BstC8I GCNNGC 1 cut(s) 1170
BstDEI CTNAG 6 cut(s) 26, 131, 444, 516, 644, 761
BstDSI CCRYGG 2 cut(s) 146, 958
BstF5I GGATG 5 cut(s) 384, 625, 643, 670, 673
BstHHI GCGC 3 cut(s) 12, 84, 123
BstKTI GATC 2 cut(s) 535, 571
BstMAI GTCTC 4 cut(s) 56, 216, 229, 440
BstMBI GATC 2 cut(s) 532, 568
BstMWI GCNNNNNNNGC 1 cut(s) 1169
BstNI CCWGG 2 cut(s) 405, 1107
BstNSI RCATGY 1 cut(s) 881
BstPAI GACNNNNGTC 1 cut(s) 452
BstSCI CCNGG 3 cut(s) 403, 976, 1105
BstSFI CTRYAG 1 cut(s) 936
BstV1I GCAGC 2 cut(s) 781, 922
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 2 cut(s) 146, 958
BtsCI GGATG 5 cut(s) 384, 625, 643, 670, 673
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 1 cut(s) 1080
BveI ACCTGC 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 1170
CaiI CAGNNNCTG 1 cut(s) 1106
CfoI GCGC 3 cut(s) 12, 84, 123
Cfr13I GGNCC 3 cut(s) 1002, 1018, 1118
Csp6I GTAC 2 cut(s) 525, 875
CspCI CAANNNNNGTGG 4 cut(s) 34, 69, 597, 632
CviQI GTAC 2 cut(s) 525, 875
DdeI CTNAG 6 cut(s) 26, 131, 444, 516, 644, 761
DpnI GATC 2 cut(s) 534, 570
DpnII GATC 2 cut(s) 532, 568
DrdI GACNNNNNNGTC 1 cut(s) 926
DseDI GACNNNNNNGTC 1 cut(s) 926
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 3 cut(s) 1002, 1018, 1118
EcoO109I RGGNCCY 1 cut(s) 1018
EcoRII CCWGG 2 cut(s) 403, 1105
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 146
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 2 cut(s) 1015, 1088
FauI CCCGC 2 cut(s) 203, 1071
Fnu4HI GCNGC 2 cut(s) 795, 936
FokI GGATG 5 cut(s) 391, 632, 650, 660, 677
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 795, 936
FspBI CTAG 2 cut(s) 230, 1023
GlaI GCGC 3 cut(s) 11, 83, 122
GluI GCNGC 2 cut(s) 795, 936
GsuI CTGGAG 1 cut(s) 1128
HaeIII GGCC 1 cut(s) 110
HapII CCGG 2 cut(s) 650, 977
HhaI GCGC 3 cut(s) 12, 84, 123
Hin6I GCGC 3 cut(s) 10, 82, 121
HinP1I GCGC 3 cut(s) 10, 82, 121
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 6 cut(s) 129, 141, 250, 440, 646, 1157
HpaII CCGG 2 cut(s) 650, 977
HphI GGTGA 4 cut(s) 16, 377, 500, 898
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 29, 134, 432, 445
Hpy188III TCNNGA 6 cut(s) 230, 239, 354, 457, 650, 1154
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 3 cut(s) 182, 185, 998
HpyAV CCTTC 2 cut(s) 264, 702
HpyCH4III ACNGT 1 cut(s) 865
HpyCH4IV ACGT 2 cut(s) 183, 996
HpyCH4V TGCA 7 cut(s) 595, 732, 747, 847, 860, 881, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 1169
HpyF3I CTNAG 6 cut(s) 26, 131, 444, 516, 644, 761
HpySE526I ACGT 2 cut(s) 183, 996
HspAI GCGC 3 cut(s) 10, 82, 121
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 2 cut(s) 532, 568
LmnI GCTCC 3 cut(s) 299, 712, 980
Lsp1109I GCAGC 2 cut(s) 781, 922
LweI GCATC 1 cut(s) 87
MaeI CTAG 2 cut(s) 230, 1023
MaeII ACGT 2 cut(s) 183, 996
MaeIII GTNAC 1 cut(s) 928
MalI GATC 2 cut(s) 534, 570
MboI GATC 2 cut(s) 532, 568
MboII GAAGA 4 cut(s) 258, 491, 799, 1079
MfeI CAATTG 2 cut(s) 289, 1128
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 1 cut(s) 163
MlsI TGGCCA 1 cut(s) 110
MluCI AATT 9 cut(s) 40, 201, 289, 417, 489, 630, 678, 964, 1128
MluNI TGGCCA 1 cut(s) 110
MlyI GAGTC 4 cut(s) 123, 244, 434, 655
MmeI TCCRAC 2 cut(s) 466, 1096
Mox20I TGGCCA 1 cut(s) 110
Mph1103I ATGCAT 1 cut(s) 883
MroI TCCGGA 1 cut(s) 649
MscI TGGCCA 1 cut(s) 110
MseI TTAA 4 cut(s) 173, 492, 543, 681
MslI CAYNNNNRTG 3 cut(s) 95, 395, 618
Msp20I TGGCCA 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 935
MspI CCGG 2 cut(s) 650, 977
MspR9I CCNGG 3 cut(s) 405, 978, 1107
MunI CAATTG 2 cut(s) 289, 1128
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 2 cut(s) 405, 1107
MwoI GCNNNNNNNGC 1 cut(s) 1169
NciI CCSGG 1 cut(s) 978
NcoI CCATGG 1 cut(s) 146
NdeII GATC 2 cut(s) 532, 568
NlaIV GGNNCC 7 cut(s) 402, 570, 714, 854, 1003, 1020, 1120
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 928
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 1 cut(s) 881
PaqCI CACCTGC 1 cut(s) 519
PctI GAATGC 1 cut(s) 881
PfeI GAWTC 2 cut(s) 141, 1157
PflMI CCANNNNNTGG 2 cut(s) 117, 1106
PkrI GCNGC 2 cut(s) 796, 937
PleI GAGTC 4 cut(s) 123, 244, 434, 654
PpsI GAGTC 4 cut(s) 123, 244, 434, 654
PpuMI RGGWCCY 1 cut(s) 1018
PshAI GACNNNNGTC 1 cut(s) 452
PshBI ATTAAT 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 1018
Psp6I CCWGG 2 cut(s) 403, 1105
PspGI CCWGG 2 cut(s) 403, 1105
PspN4I GGNNCC 7 cut(s) 402, 570, 714, 854, 1003, 1020, 1120
PspPI GGNCC 3 cut(s) 1002, 1018, 1118
PspPPI RGGWCCY 1 cut(s) 1018
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 1 cut(s) 1106
PsuI RGATCY 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 2 cut(s) 526, 876
RsaNI GTAC 2 cut(s) 525, 875
RseI CAYNNNNRTG 3 cut(s) 95, 395, 618
SaqAI TTAA 4 cut(s) 173, 492, 543, 681
SatI GCNGC 2 cut(s) 795, 936
Sau3AI GATC 2 cut(s) 532, 568
Sau96I GGNCC 3 cut(s) 1002, 1018, 1118
SchI GAGTC 4 cut(s) 123, 244, 434, 655
ScrFI CCNGG 3 cut(s) 405, 978, 1107
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 1 cut(s) 87
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 3 cut(s) 1002, 1018, 1118
SmiMI CAYNNNNRTG 3 cut(s) 95, 395, 618
Sse9I AATT 9 cut(s) 40, 201, 289, 417, 489, 630, 678, 964, 1128
SsiI CCGC 3 cut(s) 196, 338, 1078
SspI AATATT 1 cut(s) 817
SspMI CTAG 2 cut(s) 230, 1023
StyD4I CCNGG 3 cut(s) 403, 976, 1105
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 865
TaiI ACGT 2 cut(s) 186, 999
TaqI TCGA 2 cut(s) 353, 993
TasI AATT 9 cut(s) 40, 201, 289, 417, 489, 630, 678, 964, 1128
TatI WGTACW 2 cut(s) 524, 874
TfiI GAWTC 2 cut(s) 141, 1157
Tru1I TTAA 4 cut(s) 173, 492, 543, 681
Tru9I TTAA 4 cut(s) 173, 492, 543, 681
TscAI CASTG 1 cut(s) 1080
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 2 cut(s) 794, 935
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 3 cut(s) 395, 569, 1080
TspGWI ACGGA 1 cut(s) 947
TspRI CASTG 1 cut(s) 1080
Van91I CCANNNNNTGG 2 cut(s) 117, 1106
VpaK11BI GGWCC 3 cut(s) 1002, 1018, 1118
VspI ATTAAT 1 cut(s) 681
XbaI TCTAGA 1 cut(s) 229
XceI RCATGY 1 cut(s) 881
XspI CTAG 2 cut(s) 230, 1023
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.