Rroxscaffold_7G00208410

Adaptin ear-binding coat-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018970
Physical Location & Seq
Forward (+)
58433440 .. 58438733
5294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208410.1

Sequence Viewer

Length: 804 bp
ATGTTTCTCTACCTACTATACACCGAGATCGACTCATTTACTGAAATCAGATCTCAAAATCAATTGTTTCAGACTATCAAGAGTGTCGAAACTCGAGGCTTTGACGCTATACTATGTGGATTTTGCTGTGGTTGGGTTTCGATTGATCTTGATTTTTATGTTTTGTTGAGGTTTTATGCAAAGGAGTATTTGGGTTGTTCTTTTGCCAGTGTTCCTGGTTTGCATAACGTACGTCCAATTGCAGCAAATCAATATGGTGAACTACATGCTCAGGCATTTTTAAGAAAAGGAGAGCCACATCCAGTGGAACCTATAATTGATAGCAGCAGGTTTGTCCTATTTTTCCATAAAGTTGTAGATTTGCAGACAAGGCCATTTCAGAATACTAATCTGACAAGAGGATTTCAAGAAAGAACAGAAGCTTATGATTTTCAAGCAGCCCTGCATGATCACATGAACGGTTCTAGAGTAGGCCCGGTTGGATTGCGGCTGCGTGGTGGTGGATCCGGTGTCAACATCGCATGGAGATCTTGGCGGGGAAAGGCGGCGTTGATTAGGAGGGGTGGCGGCGAGTTTGGGCTCGATTTACTCAAATCCGATCGAGGGTTTAGATTTGGTTTGGAATGTTTGGAGTCACGCGAGAGGAAAAAGGTAGTTGAGAGGTGCGGCGCTTCTTGCCGGCACGATAGGGATGATGAAGGGGTGGAGGAACGATCAGCAGCTACTCGGTGCCAAGTAGGAGGCGGCGGGCTTGCGCCGAGAGATGGGATGGCCGGAATCTCCTCGTGCGAGATGGAGGCGTAG

Protein Analysis

267

Amino Acids

29.9

Weight (kDa)

7.59

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 82 - 153 4.2e-10 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 729
AccII CGCG 1 cut(s) 639
AciI CCGC 7 cut(s) 487, 535, 545, 567, 666, 744, 747
AclWI GGATC 2 cut(s) 498, 511
AcoI YGGCCR 1 cut(s) 771
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 603, 764
AgsI TTSAA 2 cut(s) 407, 434
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 2 cut(s) 422, 722
AluI AGCT 2 cut(s) 422, 722
AlwI GGATC 2 cut(s) 498, 511
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 3 cut(s) 371, 472, 771
ApeKI GCWGC 5 cut(s) 242, 324, 437, 490, 719
AspLEI GCGC 2 cut(s) 671, 757
AspS9I GGNCC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 93
BamHI GGATCC 1 cut(s) 503
BanI GGYRCC 1 cut(s) 729
BanII GRGCYC 1 cut(s) 582
BauI CACGAG 1 cut(s) 784
BbvI GCAGC 5 cut(s) 254, 336, 449, 477, 731
BccI CCATC 3 cut(s) 758, 763, 787
BciT130I CCWGG 1 cut(s) 216
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 476
BfaI CTAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 672
BfuAI ACCTGC 1 cut(s) 318
BglII AGATCT 2 cut(s) 50, 527
Bme1390I CCNGG 2 cut(s) 216, 476
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 3 cut(s) 309, 505, 731
BmrFI CCNGG 2 cut(s) 216, 476
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bpu10I CCTNAGC 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 476
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 2 cut(s) 603, 764
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 2 cut(s) 207, 302
BseBI CCWGG 1 cut(s) 216
BseGI GGATG 3 cut(s) 298, 697, 774
BseLI CCNNNNNNNGG 2 cut(s) 603, 764
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 207, 302
BseRI GAGGAG 1 cut(s) 772
BseXI GCAGC 5 cut(s) 254, 336, 449, 477, 731
Bsh1236I CGCG 1 cut(s) 639
Bsh1285I CGRYCG 1 cut(s) 601
BshFI GGCC 3 cut(s) 373, 474, 773
BshNI GGYRCC 1 cut(s) 729
BsiEI CGRYCG 1 cut(s) 601
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 4 cut(s) 476, 507, 679, 774
BsiWI CGTACG 1 cut(s) 229
BslI CCNNNNNNNGG 2 cut(s) 603, 764
BsnI GGCC 3 cut(s) 373, 474, 773
BsoBI CYCGRG 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
BspACI CCGC 7 cut(s) 487, 535, 545, 567, 666, 744, 747
BspANI GGCC 3 cut(s) 373, 474, 773
BspCNI CTCAG 1 cut(s) 283
BspFNI CGCG 1 cut(s) 639
BspLI GGNNCC 3 cut(s) 309, 505, 731
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 2 cut(s) 498, 511
BspT107I GGYRCC 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 678
BsrI ACTGG 2 cut(s) 207, 302
BssAI RCCGGY 1 cut(s) 678
BssMI GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
BssSI CACGAG 1 cut(s) 784
Bst2BI CACGAG 1 cut(s) 784
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 461
BstC8I GCNNGC 3 cut(s) 680, 749, 753
BstDEI CTNAG 1 cut(s) 270
BstF5I GGATG 3 cut(s) 298, 697, 774
BstFNI CGCG 1 cut(s) 639
BstH2I RGCGCY 1 cut(s) 672
BstHHI GCGC 2 cut(s) 671, 757
BstKTI GATC 8 cut(s) 30, 53, 148, 451, 506, 530, 601, 716
BstMBI GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
BstMCI CGRYCG 1 cut(s) 601
BstMWI GCNNNNNNNGC 2 cut(s) 370, 675
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 214, 474
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 5 cut(s) 254, 336, 449, 477, 731
BstX2I RGATCY 3 cut(s) 50, 503, 527
BstYI RGATCY 3 cut(s) 50, 503, 527
BsuRI GGCC 3 cut(s) 373, 474, 773
BtgZI GCGATG 1 cut(s) 502
BtsCI GGATG 3 cut(s) 298, 697, 774
BtsIMutI CAGTG 2 cut(s) 214, 309
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 3 cut(s) 680, 749, 753
CfoI GCGC 2 cut(s) 671, 757
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 1 cut(s) 473
CseI GACGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 230
CviAII CATG 4 cut(s) 266, 446, 454, 522
CviQI GTAC 1 cut(s) 230
DdeI CTNAG 1 cut(s) 270
DpnI GATC 8 cut(s) 29, 52, 147, 450, 505, 529, 600, 715
DpnII GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
EaeI YGGCCR 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 582
FaeI CATG 4 cut(s) 269, 449, 457, 525
FatI CATG 4 cut(s) 265, 445, 453, 521
FauI CCCGC 2 cut(s) 528, 740
FbaI TGATCA 1 cut(s) 448
FokI GGATG 3 cut(s) 285, 704, 781
FriOI GRGCYC 1 cut(s) 582
FspBI CTAG 1 cut(s) 465
GlaI GCGC 2 cut(s) 670, 756
HaeII RGCGCY 1 cut(s) 672
HaeIII GGCC 3 cut(s) 373, 474, 773
HapII CCGG 4 cut(s) 476, 507, 679, 774
HgaI GACGC 1 cut(s) 113
HhaI GCGC 2 cut(s) 671, 757
Hin1II CATG 4 cut(s) 269, 449, 457, 525
Hin6I GCGC 2 cut(s) 669, 755
HinP1I GCGC 2 cut(s) 669, 755
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 3 cut(s) 32, 632, 777
HpaII CCGG 4 cut(s) 476, 507, 679, 774
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 2 cut(s) 260, 514
Hpy188I TCNGA 5 cut(s) 50, 72, 381, 393, 598
Hpy188III TCNNGA 4 cut(s) 79, 149, 407, 465
Hpy8I GTNNAC 2 cut(s) 260, 514
HpyAV CCTTC 1 cut(s) 692
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 2 cut(s) 228, 232
HpyCH4V TGCA 5 cut(s) 179, 223, 242, 364, 445
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 675
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 2 cut(s) 228, 232
Hsp92II CATG 4 cut(s) 269, 449, 457, 525
HspAI GCGC 2 cut(s) 669, 755
KroI GCCGGC 1 cut(s) 678
KroNI GCCGGC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
Lsp1109I GCAGC 5 cut(s) 254, 336, 449, 477, 731
MaeI CTAG 1 cut(s) 465
MaeII ACGT 2 cut(s) 228, 232
MaeIII GTNAC 1 cut(s) 633
MalI GATC 8 cut(s) 29, 52, 147, 450, 505, 529, 600, 715
MboI GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
MfeI CAATTG 2 cut(s) 62, 237
MflI RGATCY 3 cut(s) 50, 503, 527
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 3 cut(s) 62, 237, 315
MlyI GAGTC 2 cut(s) 26, 641
MmeI TCCRAC 1 cut(s) 460
MroNI GCCGGC 1 cut(s) 678
MseI TTAA 1 cut(s) 281
MspI CCGG 4 cut(s) 476, 507, 679, 774
MspR9I CCNGG 2 cut(s) 216, 476
MunI CAATTG 2 cut(s) 62, 237
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 2 cut(s) 370, 675
NaeI GCCGGC 1 cut(s) 680
NciI CCSGG 1 cut(s) 476
NdeII GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
NgoMIV GCCGGC 1 cut(s) 678
NlaIII CATG 4 cut(s) 269, 449, 457, 525
NlaIV GGNNCC 3 cut(s) 309, 505, 731
NmeAIII GCCGAG 1 cut(s) 783
NmuCI GTSAC 1 cut(s) 633
NspI RCATGY 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 93
PdiI GCCGGC 1 cut(s) 680
PfeI GAWTC 1 cut(s) 777
Pfl23II CGTACG 1 cut(s) 229
Ple19I CGATCG 1 cut(s) 601
PleI GAGTC 2 cut(s) 26, 640
PpsI GAGTC 2 cut(s) 26, 640
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspLI CGTACG 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 309, 505, 731
PspPI GGNCC 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 93
PsuI RGATCY 3 cut(s) 50, 503, 527
PvuI CGATCG 1 cut(s) 601
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 281
Sau3AI GATC 8 cut(s) 27, 50, 145, 448, 503, 527, 598, 713
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 2 cut(s) 26, 641
ScrFI CCNGG 2 cut(s) 216, 476
SduI GDGCHC 1 cut(s) 582
Sfr274I CTCGAG 1 cut(s) 93
SlaI CTCGAG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 3 cut(s) 62, 237, 315
SsiI CCGC 7 cut(s) 487, 535, 545, 567, 666, 744, 747
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 2 cut(s) 214, 474
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 2 cut(s) 231, 235
TaqI TCGA 6 cut(s) 30, 87, 94, 140, 582, 601
TasI AATT 3 cut(s) 62, 237, 315
TauI GCSGC 5 cut(s) 490, 548, 570, 669, 747
TfiI GAWTC 1 cut(s) 777
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 2 cut(s) 214, 309
TseFI GTSAC 1 cut(s) 633
TseI GCWGC 5 cut(s) 242, 324, 437, 490, 719
Tsp45I GTSAC 1 cut(s) 633
TspDTI ATGAA 2 cut(s) 470, 711
TspRI CASTG 2 cut(s) 214, 309
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 269
XhoI CTCGAG 1 cut(s) 93
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.