Rroxscaffold_7G00216850

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser Thr consensus motif in nascent polypeptide chains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007802
Physical Location & Seq
Forward (+)
67509943 .. 67512671
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00216850.1

Sequence Viewer

Length: 375 bp
ATGGTGAGGCCTTCGTCATCGTCGTCCTCTACAAGCCAGGACGCCCTTGCCCTTTTCAATTCTCTGAGATCTGCTTACTCCGCCACTCCTACTGCTCTCAAGGTCATTGATCTCTATGTTGGTTTCGCTGTATCCACAGCTATCATCCAGGTTTTACAGGTAGTCTACATGGCTCTTGTTGGATCATTCCCATTTAACTCTTTTCTCTCTGGAGTACTCTCATGCATAGGGACAGCAGTCCTTGCTGTTTGCCTCCGTATTCAAGTGAACAAAGAAAACAAGGAATTTAAGGATTTAGCACCAGAGCGGGCTTTTGCAGATTTTGTTCTCTGCAATTTGGTTCTGCATTTGGTGATCATGAATTTCCTTGGATGA

Protein Analysis

124

Amino Acids

13.35

Weight (kDa)

7.71

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAD PF02109 19 - 124 1.7e-37 DAD family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 165
AciI CCGC 2 cut(s) 81, 307
AclWI GGATC 1 cut(s) 190
AcsI RAATTY 2 cut(s) 284, 361
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 216
AgsI TTSAA 2 cut(s) 58, 263
AjnI CCWGG 2 cut(s) 36, 147
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
AlwI GGATC 1 cut(s) 190
AoxI GGCC 1 cut(s) 8
ApoI RAATTY 2 cut(s) 284, 361
AsuHPI GGTGA 2 cut(s) 16, 364
BciT130I CCWGG 2 cut(s) 38, 149
BciVI GTATCC 1 cut(s) 142
BclI TGATCA 1 cut(s) 354
BfuI GTATCC 1 cut(s) 142
BglII AGATCT 1 cut(s) 68
BmcAI AGTACT 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 38, 149
BmrFI CCNGG 2 cut(s) 38, 149
BoxI GACNNNNGTC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 367
BseBI CCWGG 2 cut(s) 38, 149
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 1 cut(s) 144
BseMII CTCAG 1 cut(s) 56
BshFI GGCC 1 cut(s) 10
BslFI GGGAC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 244
BsnI GGCC 1 cut(s) 10
Bsp143I GATC 4 cut(s) 68, 109, 182, 354
BspACI CCGC 2 cut(s) 81, 307
BspANI GGCC 1 cut(s) 10
BspCNI CTCAG 1 cut(s) 57
BspHI TCATGA 1 cut(s) 357
BspPI GGATC 1 cut(s) 190
BsrBI CCGCTC 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 4 cut(s) 68, 109, 182, 354
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 367
Bst2UI CCWGG 2 cut(s) 38, 149
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 1 cut(s) 144
BstKTI GATC 4 cut(s) 71, 112, 185, 357
BstMBI GATC 4 cut(s) 68, 109, 182, 354
BstMWI GCNNNNNNNGC 2 cut(s) 80, 242
BstNI CCWGG 2 cut(s) 38, 149
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 36, 147
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuI GTATCC 1 cut(s) 142
BsuRI GGCC 1 cut(s) 10
BtsCI GGATG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 309
CciI TCATGA 1 cut(s) 357
CseI GACGC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 215
CviAII CATG 3 cut(s) 169, 222, 358
CviJI RGCY 5 cut(s) 10, 36, 140, 173, 311
CviKI_1 RGCY 5 cut(s) 10, 36, 140, 173, 311
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 1 cut(s) 65
DpnI GATC 4 cut(s) 70, 111, 184, 356
DpnII GATC 4 cut(s) 68, 109, 182, 354
EciI GGCGGA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 367
Eco147I AGGCCT 1 cut(s) 10
EcoRII CCWGG 2 cut(s) 36, 147
EcoT14I CCWWGG 1 cut(s) 367
EcoT22I ATGCAT 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 3 cut(s) 172, 225, 361
FaiI YATR 5 cut(s) 117, 170, 223, 227, 359
FaqI GGGAC 1 cut(s) 244
FatI CATG 3 cut(s) 168, 221, 357
FauI CCCGC 1 cut(s) 300
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 165
FokI GGATG 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 10
HgaI GACGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 3 cut(s) 172, 225, 361
HphI GGTGA 2 cut(s) 16, 364
Hpy166II GTNNAC 2 cut(s) 166, 268
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 2 cut(s) 210, 358
Hpy8I GTNNAC 2 cut(s) 166, 268
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 21
HpyCH4V TGCA 4 cut(s) 225, 317, 333, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 242
HpyF3I CTNAG 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 3 cut(s) 172, 225, 361
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 4 cut(s) 68, 109, 182, 354
LpnPI CCDG 7 cut(s) 23, 50, 134, 143, 161, 195, 315
MalI GATC 4 cut(s) 70, 111, 184, 356
MbiI CCGCTC 1 cut(s) 307
MboI GATC 4 cut(s) 68, 109, 182, 354
MflI RGATCY 1 cut(s) 68
MluCI AATT 4 cut(s) 58, 284, 334, 361
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 2 cut(s) 37, 263
Mph1103I ATGCAT 1 cut(s) 227
MseI TTAA 2 cut(s) 195, 288
MspR9I CCNGG 2 cut(s) 38, 149
MvaI CCWGG 2 cut(s) 38, 149
MwoI GCNNNNNNNGC 2 cut(s) 80, 242
NdeII GATC 4 cut(s) 68, 109, 182, 354
NlaIII CATG 3 cut(s) 172, 225, 361
NsiI ATGCAT 1 cut(s) 227
PagI TCATGA 1 cut(s) 357
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PshAI GACNNNNGTC 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 36, 147
PspGI CCWGG 2 cut(s) 36, 147
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 195, 288
Sau3AI GATC 4 cut(s) 68, 109, 182, 354
ScaI AGTACT 1 cut(s) 216
ScrFI CCNGG 2 cut(s) 38, 149
SetI ASST 4 cut(s) 105, 142, 153, 162
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 4 cut(s) 58, 284, 334, 361
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 2 cut(s) 81, 307
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 36, 147
StyI CCWWGG 1 cut(s) 367
TasI AATT 4 cut(s) 58, 284, 334, 361
TatI WGTACW 1 cut(s) 214
Tru1I TTAA 2 cut(s) 195, 288
Tru9I TTAA 2 cut(s) 195, 288
TspDTI ATGAA 1 cut(s) 374
TspGWI ACGGA 1 cut(s) 245
XapI RAATTY 2 cut(s) 284, 361
XmiI GTMKAC 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 216
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.