Rorug01G0002300

DUF167

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006289
Physical Location & Seq
Reverse (-)
522285 .. 523703
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0002300.1

Sequence Viewer

Length: 615 bp
ATGGTGACAGGAAACCTTACAAATGTGATCAAAGTAATATATAATGTCATCTTTTTGAATATGTTTATGAATCCAAAAATGAATTCAGATGAGTTACTTACCTCCAATGCATTTTTTCAGGAAATATACAAGAACGGAGGAAGAAAATTTGGGATTAAAGGCATAGGGAATTTGGGTTGTATACCGGGCTTCAGAACAGTTAAACCAGGAAACACGAGCACCTGTAATGAAGAAGTAAATGCACTTTCAAAATTCCACAATAGGGTACTTGCTAAAGCCCTCCTGGAGCTGAAAGGACAGCTCCAAGACTTCATATACTCGCATCCAAATTTCTACTCTTACGCAAATGACGTAGTTCATAATCCATCAAAACATGGTTTCAAGGAAGCAGAGATGGCATGCTGTGGCTCTGGTCCATACAGAAGAATTAAAAGCTGCGGAGGGAAGCGAGGTGTGACTGAGTATCAGTTATGTGACAATGTTACTGACTATGTCTACTTTGATTCCGGCCATCCCACAGAAAGGTATAACCAGAAACTTTCCAAGTTATGGTGGAGCCATACTTCTGATGTCACAGCACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF167

Protein Analysis

204

Amino Acids

23.18

Weight (kDa)

8.95

Isoelectric Point (pI)

21.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 183 9.6e-07 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 549
AccI GTMKAC 2 cut(s) 181, 495
AciI CCGC 1 cut(s) 438
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 5 cut(s) 82, 146, 169, 251, 328
AcuI CTGAAG 1 cut(s) 175
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 3 cut(s) 262, 522, 549
AgsI TTSAA 5 cut(s) 58, 249, 382, 595, 608
AjnI CCWGG 2 cut(s) 205, 282
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
AluBI AGCT 4 cut(s) 289, 301, 435, 601
AluI AGCT 4 cut(s) 289, 301, 435, 601
Alw21I GWGCWC 1 cut(s) 221
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 435
ApoI RAATTY 5 cut(s) 82, 146, 169, 251, 328
AspS9I GGNCC 1 cut(s) 413
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 413
BauI CACGAG 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 422
BccI CCATC 3 cut(s) 373, 388, 519
BciT130I CCWGG 2 cut(s) 207, 284
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 1 cut(s) 186
BisI GCNGC 1 cut(s) 436
BlsI GCNGC 1 cut(s) 437
Bme1390I CCNGG 3 cut(s) 186, 207, 284
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 3 cut(s) 186, 207, 284
BmsI GCATC 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 522, 549
BseBI CCWGG 2 cut(s) 207, 284
BseGI GGATG 2 cut(s) 322, 511
BseLI CCNNNNNNNGG 3 cut(s) 262, 522, 549
BseMII CTCAG 1 cut(s) 450
BseXI GCAGC 1 cut(s) 422
BshFI GGCC 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 2 cut(s) 185, 507
BslI CCNNNNNNNGG 3 cut(s) 262, 522, 549
BsnI GGCC 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 1 cut(s) 438
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 557
BssMI GATC 1 cut(s) 27
BssNAI GTATAC 1 cut(s) 182
BssSI CACGAG 1 cut(s) 214
Bst1107I GTATAC 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 2 cut(s) 207, 284
Bst4CI ACNGT 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 322, 511
BstKTI GATC 1 cut(s) 30
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 2 cut(s) 207, 284
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 3 cut(s) 184, 205, 282
BstV1I GCAGC 1 cut(s) 422
BstZ17I GTATAC 1 cut(s) 182
BsuRI GGCC 1 cut(s) 510
BtsCI GGATG 2 cut(s) 322, 511
Cac8I GCNNGC 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 1 cut(s) 266
CviAII CATG 2 cut(s) 374, 399
CviJI RGCY 9 cut(s) 189, 278, 289, 301, 408, 435, 510, 558, 601
CviKI_1 RGCY 9 cut(s) 189, 278, 289, 301, 408, 435, 510, 558, 601
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 1 cut(s) 459
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
EaeI YGGCCR 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 413
Eco57I CTGAAG 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 205, 282
EcoT22I ATGCAT 1 cut(s) 112
FaeI CATG 2 cut(s) 377, 402
FatI CATG 2 cut(s) 373, 398
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 2 cut(s) 181, 495
Fnu4HI GCNGC 1 cut(s) 436
FokI GGATG 2 cut(s) 309, 498
Fsp4HI GCNGC 1 cut(s) 436
GluI GCNGC 1 cut(s) 436
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 1 cut(s) 510
HapII CCGG 2 cut(s) 185, 507
Hin1II CATG 2 cut(s) 377, 402
HinfI GANTC 2 cut(s) 70, 503
HpaII CCGG 2 cut(s) 185, 507
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 182, 496
Hpy188I TCNGA 3 cut(s) 88, 194, 568
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 2 cut(s) 182, 496
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 2 cut(s) 351, 580
HpyCH4V TGCA 2 cut(s) 110, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 2 cut(s) 351, 580
Hsp92II CATG 2 cut(s) 377, 402
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 1 cut(s) 27
LmnI GCTCC 3 cut(s) 286, 306, 555
Lsp1109I GCAGC 1 cut(s) 422
LweI GCATC 1 cut(s) 331
MaeII ACGT 2 cut(s) 351, 580
MaeIII GTNAC 7 cut(s) 4, 93, 454, 473, 481, 571, 581
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 3 cut(s) 153, 242, 435
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 7 cut(s) 82, 146, 169, 251, 328, 426, 589
MnlI CCTC 5 cut(s) 112, 131, 290, 434, 443
Mph1103I ATGCAT 1 cut(s) 112
MseI TTAA 3 cut(s) 156, 201, 429
MspI CCGG 2 cut(s) 185, 507
MspR9I CCNGG 3 cut(s) 186, 207, 284
MvaI CCWGG 2 cut(s) 207, 284
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 186
NdeII GATC 1 cut(s) 27
NlaIII CATG 2 cut(s) 377, 402
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 4 cut(s) 4, 454, 473, 571
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 1 cut(s) 402
PaeI GCATGC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 2 cut(s) 70, 503
PflFI GACNNNGTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 549
PfoI TCCNGGA 1 cut(s) 282
PkrI GCNGC 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 205, 282
PspGI CCWGG 2 cut(s) 205, 282
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 1 cut(s) 413
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 156, 201, 429
SatI GCNGC 1 cut(s) 436
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 1 cut(s) 413
ScrFI CCNGG 3 cut(s) 186, 207, 284
SduI GDGCHC 1 cut(s) 221
SfaNI GCATC 1 cut(s) 331
SinI GGWCC 1 cut(s) 413
SphI GCATGC 1 cut(s) 402
Sse9I AATT 7 cut(s) 82, 146, 169, 251, 328, 426, 589
SsiI CCGC 1 cut(s) 438
StyD4I CCNGG 3 cut(s) 184, 205, 282
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 2 cut(s) 354, 583
TasI AATT 7 cut(s) 82, 146, 169, 251, 328, 426, 589
TfiI GAWTC 2 cut(s) 70, 503
Tru1I TTAA 3 cut(s) 156, 201, 429
Tru9I TTAA 3 cut(s) 156, 201, 429
TseFI GTSAC 4 cut(s) 4, 454, 473, 571
TseI GCWGC 1 cut(s) 435
Tsp45I GTSAC 4 cut(s) 4, 454, 473, 571
TspDTI ATGAA 5 cut(s) 83, 95, 243, 301, 347
TspGWI ACGGA 1 cut(s) 150
Tth111I GACNNNGTC 1 cut(s) 491
Van91I CCANNNNNTGG 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 5 cut(s) 82, 146, 169, 251, 328
XceI RCATGY 1 cut(s) 402
XmiI GTMKAC 2 cut(s) 181, 495
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.