Rorug01G0039600

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012042
Physical Location & Seq
Forward (+)
6492730 .. 6496785
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0039600.1

Sequence Viewer

Length: 4056 bp
ATGGCAGTAGGAGAGATATTTCTTGCGGCATTTCTTCAGGTGCTGCTCAACAGGTTGGTCTCTGGCGACTTTGTCAACTTCGCAACCCAAAAGGGGATTGGCAGAAAGCTGGAGAAATGGAGGGAAACTTTGTCTACCATTGTGGCTGTGCTGTATGATGCAGAGGAGAAGCAACTAACGAGCAAGGCAGTGGAGCTATGGCTGGATGATCTCAAGGACTTGGCTTATGATGTGGAGGACATACTGGACAAGTTTTCCACTAAGATGTTACGACGCAAGGTCAAGCAGCGAAATCGGGCTGGCACAAGCAAGGTACGAGGGATCTTCTCTAAAGTTGCTAAAGTTAAATTCAACTTGAATATGAACTCTGAAATCGAGGAGATTGATGATCGGTTACTTCGTATATCTCAAAGGAAAAGCGACCTGGGGTTAATATATATTGAGCCTAGGTCCACTATGATGGGGCAAAGGTTGCCAAGTTCATATGTGCTAGATGGACCTGTAGTTGGAAGGGGTGAAGACAAAAAAACAATTCTTGAATTTTTGTTTAGATATCATGAGCCTTCGATCACCAGTTTTCACGTAGTTGCAATTGTTGGTATGCCAGGAATCGGCAAGACAACACTTGCAGGACATGTATTCACTGCAGTTAAGCAATTTAATCCCAAGGTGTGGGTATCTGTGTCTGATAACTTCAATCTTGAGAGAGTGACGAAAACAATTTATAAGTTAGTCACATCCCAACATTGTGATTTGGATGAGTTCTGTCAAGTTCAAGATAGTTTGCGTGAGGCATTAGAAGGGAAAAGGTTTTTAATCGTTCTAGATGATGTGTGGACCACATGTGACTACAATTCATGGAGAAGTTTGCAGTCCACTTTTCATCTTGGAGCACAGGGAAGTAAGATAATTGTGACAACGCGGGATAAAGAAGTTGCAAAATTGATGGGAGCTACAGAAGTTTATGATTTGAAGACTCTGTCTGACGAAGACTGTTTGCAGGTATTTCAGCAACATGTCAACAATGTCAAACCACCAAGTTTTGAGTTAACTAAGAAAATCGTTAAAAATTGTGATGGATTGCCTTTGGCTGCGAAGACCCTCGGTGGTATTTTACGTTGTAAAGAAACTGACAAATGGGAGGAAGTATTGAATGATAGATTATGGACTGTGTGTGATAATAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCATTATCTCCCTTCAAGTTTGAAGAGGTGCTTTGCCTATTGCTCCATACTACCAAATGACTATGAATTCAAGAAAAAGCAGTTAATCCTTCTATGGATGGCAGAGGGTTTGCTTCAGCAATCAGAAGGAAGTACACAGATGGAAGATATTGGTGACAACTATTTTGTCGAGCTATTATCTCGGTCACTATTTCAAAACTCGGGCAAGAAAAATTCACGATATGTAATGCATGACCTTGTTGGAGATTTGGCACGATGGGCTGCTGGAGGCACATTTTTCAGACTGCAGGATAAATGGGATGGTAGGTGTGCTCCAAGGATCCGACATTCATCTTACATTTCTGGTCTCTATGATGGGGATAAAAAATTTGAGAATGTTTTAAAATTCAAACGGTTGCGGACTTTCTTACCACTTTCAATTTCACAAGGTCCACAAGGTCCACAAGGTCGTCAGCATTATCTATCTCGCAAGGTTGCTTTTGATTTAGTACCAAAATTGGAGTCTTTACGGGTGCTCTCTTTTAATGGCTATCAAATAACTGAGTTGCCAGATTCAATTGGTCATTTGAAGCATCTACGGTACCTTGATCTTTCTCACACACAAATAAGTAGTTTGCCTGAATCAATCACCACTCTTTTCAACTTGCAGACATTGATTTTGAAAAGTTGTTCCCATTTGAAGGCTCTACCGACGAACATTAGCAATCTAACTAATCTACGGCATCTCAACAATTCATATGTACCGTCATTGGAAGGAATGCCTGCACAACTAGGTCAGCTGACTAATCTACAAACACTGTGCAAGTTTTTGGTGGGCAAAGGTAGCGAGTCAGGGATAAGCGAGATAGGGCCCTTATTGAATCTTCGAGGGACATTGTCAATTTCAAGACTGCAGAAGGTGAATGATGTTGAGGAAGCAAGGAGGGCTGACTTATCTGGAAAGAAAGGGCTTGATGCATTGGAACTAGAATGGAGTGGCATAGGAAACAAGGAATTAGAGGTGCTTGACATGTTAAAACCTCATGGAAAGCTCAGAAAACTCATGATCAAGGGCTATGGTGGTTTGACGTTTCCAAAATGGATAGGATATCCTTCATTCTTTGAGATGATGGTTGTAAGGTTAGAGAATTGTAAAAAATGTCGGTTCTTGCCTCCACTTGGACAATTATGTTCTCTCAAAGAACTGTACATAACTGGAATGTCTGAAGTAGAAAGCGTGGGTCCTGAGTTTTATGGAGATGGTAGCTTGCCTTTTCCACTGTTGGAGACCCTTGAATTTGAGGACATGAAACATTGGAAGGAGTGGTTCCCGTGGGAACAAGTAAAAGGGAAGGGAGTTTTCCCTTGCTTAACAATGCTATCTATCTCAAGATGCCCCAATTTAGAGGGTAGGTTACCCACAAACCTTGTTTATCTATCCAACCTTGTGATTCGTGAATGCATGCAGCTGGTGGTTTCGATTTCCAATTATAAACAACTATGTCAACTAAACATTGACAATTGCAAGGCAGTGGTGCACAGAAGTTCAGTTGAGTTCAAGTTACTCAAGGTTCTAAAGCTTGCTAGTATTTCAGAGTTCAGACTCCAAATAGAGGAATTCATGAGAGGATTGACAAGTGTTACGTATTTACAGATTACTGGTTGCGAGGAGCTGGCATCTTCATGGCAAAGTGAGGATAGAGTACTCCAACACTTAATGTCACTTCATGAGCTCAGAATATATAAATGCCAAAGTCTAGTATCTTTTCCAGAAGGTAGTCTGCCATCTTCTCTTAAATATATATGGATTCAGCAGTGCCATTCCCTAACATCTTTTGCAAGATATCAGATACCCTCAAGTGTAACAAGAATAGAGATTCGATTGTGCGAAAAATTGAAATCATTGCTAGGGGAGAAGGGTGAGGCTTCATTGCCTTCTTCTTCTTCTCCTTGTTTGATGCAAGACGAATCCTTTCTTGAGTACTTGACCATTACTGGTTGTCCATCCCTCACATCCTTGTCATTCAATGGCCATGTACCAAGGGCTCTTAAACACCTTCAAATTCATCGAAATGACCAACTGGAGTCAATAACAGAAAGCTTCTACAAGAACAATTGTCTCGAACTTTTTGATATCTTCCGTTGCTCAAGTCTTAAATCCTTACCGGAAGGGATATGCCACCTCACCAATCTTCGATTATTTGAGATTTGGGACTGTAAAAGTCTTGTTTCCTTCACGAGAGGAGGGTCGCCAACAGGAGCCTCCAATCTAAACTCTCTTAAGATACTCAGGATTGGTCATTGTGAAGGTCTTGTGCCTTTTCTACGAGAGGGGGGGTTTACTGCCAATGTAACATCAATTGATATAGCAGGTTTCGATATTTTTCAACTCTTCAGTTGGGGGCTGCATCACTTAACCTCTCTTAGACAGTTGAGGATCCAAGGTGTTACAGATGTGGTCTCTTTTCCACCTGATGAGAATCAAATACTGCAGCTCCCCAAATCTCTCACAAGACTCACGTTCGGATACTTTGTAAATTTGATCAAACTCAGCAAGGGTCTTCAATTCCTCACCTCTCTTCAATCTCTTGAAATTTGGGACTGTCCTAAGCTAGCATTCATTCCAGACACCGGCCTGCCTCTTTCACTTCAGAAACTTAGTATCAAAAGATGCCTGGAGATAGAAGAGAAATGCAAACAAGGAAGAGGAAGATACTGGCCAAAAATATTACGCATCCCTCACATACAGCTTGGAGGCTCAGACTCAGAAGCCAACTCGGATATTGATGAGGACAGCAGCTGGAACACGTATATAAGTGAAAAGTTGCAAGAGATGTCTAGTGCAGACTCTCCACCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

1351

Amino Acids

153.19

Weight (kDa)

8.5

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 99 4.6e-22 Rx N-terminal domain
NB-ARC PF00931 175 - 342 4.4e-35 NB-ARC domain
WHD_DRP PF23559 421 - 489 7e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 570 - 860 7.4e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 684 - 805 9e-32 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13810
fragaria_vesca FvH4_3g03100 FvH4_7g02860
malus_domestica MD02G1289200.v1.1 MD07G1038000.v1.1
prunus_persica Prupe.2G033200_v2.0.a1
pyrus_communis pycom07g02630
rosa_chinensis RchiOBHm_Chr1g0323791
rosa_laevigata RLG00000030296
rosa_multiflora Rmu_co8205106.1_g000001 Rmu_sc0000252.1_g000031 Rmu_sc0002634.1_g000015
rosa_roxburghii Rroxscaffold_4G00326410
rosa_rugosa Rorug01G0039600
rosa_samantha Rh1AG056800 Rh1BG047800 Rh1CG058100 Rh1DG061900
rosa_wichuraiana Rw1G004840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 726, 2685
Acc36I ACCTGC 2 cut(s) 991, 3554
Acc65I GGTACC 1 cut(s) 1794
AccB1I GGYRCC 1 cut(s) 1794
AccB7I CCANNNNNTGG 1 cut(s) 672
AccI GTMKAC 1 cut(s) 134
AccII CGCG 1 cut(s) 922
AciI CCGC 3 cut(s) 26, 922, 1612
AclWI GGATC 5 cut(s) 329, 1528, 1541, 3625, 3638
AcoI YGGCCR 2 cut(s) 3223, 3911
AcuI CTGAAG 5 cut(s) 20, 1313, 2439, 3571, 3827
AfaI GTAC 9 cut(s) 315, 1348, 1704, 1796, 1956, 2402, 2898, 3176, 3231
AfiI CCNNNNNNNGG 8 cut(s) 93, 506, 611, 672, 1894, 2372, 2806, 3824
AflII CTTAAG 1 cut(s) 3473
AflIII ACRYGT 5 cut(s) 634, 842, 1015, 2223, 3999
AhdI GACNNNNNGTC 1 cut(s) 278
AjnI CCWGG 3 cut(s) 423, 604, 3867
Alw21I GWGCWC 5 cut(s) 895, 1528, 1731, 2733, 2928
Alw26I GTCTC 5 cut(s) 64, 1565, 2474, 3317, 3658
Alw44I GTGCAC 1 cut(s) 2729
AlwI GGATC 5 cut(s) 329, 1528, 1541, 3625, 3638
AlwNI CAGNNNCTG 2 cut(s) 43, 3993
Ama87I CYCGRG 1 cut(s) 1414
AoxI GGCC 4 cut(s) 2063, 3223, 3826, 3911
ApaI GGGCCC 1 cut(s) 2067
ApaLI GTGCAC 1 cut(s) 2729
ApeKI GCWGC 8 cut(s) 43, 286, 1091, 1475, 2659, 3598, 3685, 3990
ArsI GACNNNNNNTTYG 6 cut(s) 1361, 1393, 1700, 1732, 1855, 1887
Asp700I GAANNNNTTC 1 cut(s) 3165
Asp718I GGTACC 1 cut(s) 1794
AspA2I CCTAGG 1 cut(s) 446
AspS9I GGNCC 8 cut(s) 450, 497, 837, 1643, 1652, 2063, 2064, 2435
AsuHPI GGTGA 8 cut(s) 527, 562, 1379, 1834, 2125, 3125, 3370, 3757
AsuNHI GCTAGC 1 cut(s) 3805
AvaI CYCGRG 1 cut(s) 1414
AvaII GGWCC 6 cut(s) 450, 497, 837, 1643, 1652, 2435
AvrII CCTAGG 1 cut(s) 446
BaeGI GKGCMC 2 cut(s) 2067, 2733
BalI TGGCCA 2 cut(s) 3225, 3913
BamHI GGATCC 2 cut(s) 1533, 3630
BanI GGYRCC 1 cut(s) 1794
BanII GRGCYC 3 cut(s) 2067, 2928, 3241
BauI CACGAG 1 cut(s) 3430
BbsI GAAGAC 5 cut(s) 525, 980, 996, 1103, 3746
Bbv12I GWGCWC 5 cut(s) 895, 1528, 1731, 2733, 2928
BbvI GCAGC 8 cut(s) 30, 298, 1078, 1462, 2671, 3585, 3697, 4002
BceAI ACGGC 1 cut(s) 1949
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 3 cut(s) 425, 606, 3869
BciVI GTATCC 1 cut(s) 3713
BclI TGATCA 2 cut(s) 2259, 3735
BcoDI GTCTC 5 cut(s) 64, 1565, 2474, 3317, 3658
BfmI CTRYAG 6 cut(s) 501, 645, 954, 1499, 2105, 3683
BfrI CTTAAG 1 cut(s) 3473
BfuAI ACCTGC 2 cut(s) 991, 3554
BfuI GTATCC 1 cut(s) 3713
BisI GCNGC 9 cut(s) 27, 44, 287, 1092, 1476, 2660, 3599, 3686, 3991
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 9 cut(s) 28, 45, 288, 1093, 1477, 2661, 3600, 3687, 3992
BmcAI AGTACT 2 cut(s) 2898, 3176
Bme1390I CCNGG 3 cut(s) 425, 606, 3869
Bme18I GGWCC 6 cut(s) 450, 497, 837, 1643, 1652, 2435
BmeRI GACNNNNNGTC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 1414
BmgT120I GGNCC 8 cut(s) 450, 497, 837, 1643, 1652, 2063, 2064, 2435
BmiI GGNNCC 7 cut(s) 1535, 1796, 2065, 2436, 2522, 3454, 3632
BmrFI CCNGG 3 cut(s) 425, 606, 3869
BmrI ACTGGG 1 cut(s) 1190
BmtI GCTAGC 1 cut(s) 3809
BmuI ACTGGG 1 cut(s) 1190
BpiI GAAGAC 5 cut(s) 525, 980, 996, 1103, 3746
BpmI CTGGAG 4 cut(s) 131, 1500, 3296, 3890
Bpu10I CCTNAGC 1 cut(s) 3801
BpuEI CTTGAG 7 cut(s) 197, 722, 2566, 2744, 3034, 3191, 3325
BsaAI YACGTR 3 cut(s) 583, 2838, 4002
BsaBI GATNNNNATC 1 cut(s) 3672
BsaI GGTCTC 4 cut(s) 64, 1565, 2474, 3658
BsaJI CCNNGG 8 cut(s) 424, 446, 666, 1102, 1529, 2525, 3233, 3634
BsaWI WCCGGW 1 cut(s) 3358
BsaXI ACNNNNNCTCC 2 cut(s) 3672, 3702
Bsc4I CCNNNNNNNGG 8 cut(s) 93, 506, 611, 672, 1894, 2372, 2806, 3824
Bse118I RCCGGY 1 cut(s) 3824
Bse1I ACTGG 8 cut(s) 249, 573, 1196, 2413, 2857, 3193, 3279, 3914
Bse3DI GCAATG 2 cut(s) 3095, 3122
Bse8I GATNNNNATC 1 cut(s) 3672
BseBI CCWGG 3 cut(s) 425, 606, 3869
BseDI CCNNGG 8 cut(s) 424, 446, 666, 1102, 1529, 2525, 3233, 3634
BseGI GGATG 8 cut(s) 211, 737, 763, 1317, 1519, 3197, 3206, 3927
BseJI GATNNNNATC 1 cut(s) 3672
BseLI CCNNNNNNNGG 8 cut(s) 93, 506, 611, 672, 1894, 2372, 2806, 3824
BseMI GCAATG 2 cut(s) 3095, 3122
BseMII CTCAG 8 cut(s) 1746, 2260, 2430, 2941, 3496, 3757, 3966, 3972
BseNI ACTGG 8 cut(s) 249, 573, 1196, 2413, 2857, 3193, 3279, 3914
BseRI GAGGAG 4 cut(s) 179, 392, 2876, 3450
BseSI GKGCMC 2 cut(s) 2067, 2733
BseXI GCAGC 8 cut(s) 30, 298, 1078, 1462, 2671, 3585, 3697, 4002
BsgI GTGCAG 2 cut(s) 1962, 4056
Bsh1236I CGCG 1 cut(s) 922
BshFI GGCC 4 cut(s) 2065, 3225, 3828, 3913
BshNI GGYRCC 1 cut(s) 1794
BsiHKAI GWGCWC 5 cut(s) 895, 1528, 1731, 2733, 2928
BsiHKCI CYCGRG 1 cut(s) 1414
BsiSI CCGG 2 cut(s) 3359, 3825
BslFI GGGAC 3 cut(s) 2098, 3419, 3806
BslI CCNNNNNNNGG 8 cut(s) 93, 506, 611, 672, 1894, 2372, 2806, 3824
BsmAI GTCTC 5 cut(s) 64, 1565, 2474, 3317, 3658
BsmFI GGGAC 3 cut(s) 2098, 3419, 3806
BsmI GAATGC 4 cut(s) 1188, 1977, 2657, 3809
BsnI GGCC 4 cut(s) 2065, 3225, 3828, 3913
Bso31I GGTCTC 4 cut(s) 64, 1565, 2474, 3658
BsoBI CYCGRG 1 cut(s) 1414
Bsp120I GGGCCC 1 cut(s) 2063
Bsp1286I GDGCHC 7 cut(s) 895, 1528, 1731, 2067, 2733, 2928, 3241
Bsp1407I TGTACA 1 cut(s) 2400
Bsp143I GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
BspACI CCGC 3 cut(s) 26, 922, 1612
BspANI GGCC 4 cut(s) 2065, 3225, 3828, 3913
BspCNI CTCAG 8 cut(s) 1747, 2259, 2431, 2940, 3495, 3756, 3965, 3971
BspFNI CGCG 1 cut(s) 922
BspHI TCATGA 4 cut(s) 556, 2256, 2814, 2920
BspLI GGNNCC 7 cut(s) 1535, 1796, 2065, 2436, 2522, 3454, 3632
BspMAI CTGCAG 4 cut(s) 649, 1503, 2109, 3687
BspMI ACCTGC 2 cut(s) 991, 3554
BspOI GCTAGC 1 cut(s) 3809
BspPI GGATC 5 cut(s) 329, 1528, 1541, 3625, 3638
BspT107I GGYRCC 1 cut(s) 1794
BspTI CTTAAG 1 cut(s) 3473
BspTNI GGTCTC 4 cut(s) 64, 1565, 2474, 3658
BsrDI GCAATG 2 cut(s) 3095, 3122
BsrFI RCCGGY 1 cut(s) 3824
BsrGI TGTACA 1 cut(s) 2400
BsrI ACTGG 8 cut(s) 249, 573, 1196, 2413, 2857, 3193, 3279, 3914
BssAI RCCGGY 1 cut(s) 3824
BssECI CCNNGG 8 cut(s) 424, 446, 666, 1102, 1529, 2525, 3233, 3634
BssMI GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
BssSI CACGAG 1 cut(s) 3430
BssT1I CCWWGG 5 cut(s) 446, 666, 1529, 3233, 3634
Bst2BI CACGAG 1 cut(s) 3430
Bst2UI CCWGG 3 cut(s) 425, 606, 3869
Bst6I CTCTTC 5 cut(s) 1232, 3590, 3777, 3873, 3892
BstAFI CTTAAG 1 cut(s) 3473
BstAPI GCANNNNNTGC 1 cut(s) 472
BstAUI TGTACA 1 cut(s) 2400
BstBAI YACGTR 3 cut(s) 583, 2838, 4002
BstC8I GCNNGC 8 cut(s) 301, 1977, 2462, 2657, 2775, 2868, 3807, 3830
BstDSI CCRYGG 1 cut(s) 2525
BstEII GGTNACC 1 cut(s) 2607
BstF5I GGATG 8 cut(s) 211, 737, 763, 1317, 1519, 3197, 3206, 3927
BstFNI CGCG 1 cut(s) 922
BstKTI GATC 9 cut(s) 211, 324, 391, 570, 1536, 1804, 2262, 3633, 3738
BstMAI GTCTC 5 cut(s) 64, 1565, 2474, 3317, 3658
BstMBI GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
BstMWI GCNNNNNNNGC 4 cut(s) 472, 1472, 2037, 2138
BstNI CCWGG 3 cut(s) 425, 606, 3869
BstNSI RCATGY 5 cut(s) 638, 846, 1019, 2227, 2659
BstPI GGTNACC 1 cut(s) 2607
BstSCI CCNGG 3 cut(s) 423, 604, 3867
BstSFI CTRYAG 6 cut(s) 501, 645, 954, 1499, 2105, 3683
BstSLI GKGCMC 2 cut(s) 2067, 2733
BstSNI TACGTA 1 cut(s) 2838
BstUI CGCG 1 cut(s) 922
BstV1I GCAGC 8 cut(s) 30, 298, 1078, 1462, 2671, 3585, 3697, 4002
BstV2I GAAGAC 5 cut(s) 525, 980, 996, 1103, 3746
BstX2I RGATCY 3 cut(s) 321, 1533, 3630
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 3 cut(s) 321, 1533, 3630
BsuI GTATCC 1 cut(s) 3713
BsuRI GGCC 4 cut(s) 2065, 3225, 3828, 3913
BtgI CCRYGG 1 cut(s) 2525
BtsCI GGATG 8 cut(s) 211, 737, 763, 1317, 1519, 3197, 3206, 3927
BtsI GCAGTG 4 cut(s) 195, 642, 2730, 3014
BtsIMutI CAGTG 6 cut(s) 195, 642, 2009, 2471, 2730, 3014
BveI ACCTGC 2 cut(s) 991, 3554
Cac8I GCNNGC 8 cut(s) 301, 1977, 2462, 2657, 2775, 2868, 3807, 3830
CaiI CAGNNNCTG 2 cut(s) 43, 3993
CciI TCATGA 4 cut(s) 556, 2256, 2814, 2920
Cfr10I RCCGGY 1 cut(s) 3824
Cfr13I GGNCC 8 cut(s) 450, 497, 837, 1643, 1652, 2063, 2064, 2435
CseI GACGC 1 cut(s) 282
Csp6I GTAC 9 cut(s) 314, 1347, 1703, 1795, 1955, 2401, 2897, 3175, 3230
CspCI CAANNNNNGTGG 2 cut(s) 2602, 2637
CviQI GTAC 9 cut(s) 314, 1347, 1703, 1795, 1955, 2401, 2897, 3175, 3230
DpnI GATC 9 cut(s) 210, 323, 390, 569, 1535, 1803, 2261, 3632, 3737
DpnII GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
DraI TTTAAA 1 cut(s) 1596
DriI GACNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 2 cut(s) 3223, 3911
Eam1104I CTCTTC 5 cut(s) 1232, 3590, 3777, 3873, 3892
Eam1105I GACNNNNNGTC 1 cut(s) 278
EarI CTCTTC 5 cut(s) 1232, 3590, 3777, 3873, 3892
Ecl136II GAGCTC 1 cut(s) 2926
Eco105I TACGTA 1 cut(s) 2838
Eco130I CCWWGG 5 cut(s) 446, 666, 1529, 3233, 3634
Eco24I GRGCYC 3 cut(s) 2067, 2928, 3241
Eco31I GGTCTC 4 cut(s) 64, 1565, 2474, 3658
Eco32I GATATC 4 cut(s) 554, 2303, 3038, 3328
Eco47I GGWCC 6 cut(s) 450, 497, 837, 1643, 1652, 2435
Eco53kI GAGCTC 1 cut(s) 2926
Eco57I CTGAAG 5 cut(s) 20, 1313, 2439, 3571, 3827
Eco88I CYCGRG 1 cut(s) 1414
Eco91I GGTNACC 1 cut(s) 2607
EcoICRI GAGCTC 1 cut(s) 2926
EcoO109I RGGNCCY 3 cut(s) 2063, 2064, 2435
EcoO65I GGTNACC 1 cut(s) 2607
EcoRI GAATTC 2 cut(s) 1280, 2810
EcoRII CCWGG 3 cut(s) 423, 604, 3867
EcoRV GATATC 4 cut(s) 554, 2303, 3038, 3328
EcoT14I CCWWGG 5 cut(s) 446, 666, 1529, 3233, 3634
EcoT22I ATGCAT 3 cut(s) 1446, 2173, 2657
EcoT38I GRGCYC 3 cut(s) 2067, 2928, 3241
ErhI CCWWGG 5 cut(s) 446, 666, 1529, 3233, 3634
FalI AAGNNNNNCTT 2 cut(s) 1229, 1261
FaqI GGGAC 3 cut(s) 2098, 3419, 3806
FauI CCCGC 1 cut(s) 915
FauNDI CATATG 2 cut(s) 484, 1951
FbaI TGATCA 2 cut(s) 2259, 3735
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 9 cut(s) 27, 44, 287, 1092, 1476, 2660, 3599, 3686, 3991
FokI GGATG 8 cut(s) 218, 724, 770, 1324, 1526, 3184, 3193, 3914
FriOI GRGCYC 3 cut(s) 2067, 2928, 3241
Fsp4HI GCNGC 9 cut(s) 27, 44, 287, 1092, 1476, 2660, 3599, 3686, 3991
GluI GCNGC 9 cut(s) 27, 44, 287, 1092, 1476, 2660, 3599, 3686, 3991
GsuI CTGGAG 4 cut(s) 131, 1500, 3296, 3890
HaeIII GGCC 4 cut(s) 2065, 3225, 3828, 3913
HapII CCGG 2 cut(s) 3359, 3825
HgaI GACGC 1 cut(s) 282
HincII GTYRAC 4 cut(s) 76, 1021, 1050, 2699
HindII GTYRAC 4 cut(s) 76, 1021, 1050, 2699
HindIII AAGCTT 2 cut(s) 2771, 3292
HpaI GTTAAC 1 cut(s) 1050
HpaII CCGG 2 cut(s) 3359, 3825
HphI GGTGA 8 cut(s) 527, 562, 1379, 1834, 2125, 3125, 3370, 3757
Hpy99I CGWCG 2 cut(s) 276, 1909
HpyCH4IV ACGT 6 cut(s) 582, 1117, 2282, 2837, 3713, 4001
HpyF10VI GCNNNNNNNGC 4 cut(s) 472, 1472, 2037, 2138
HpySE526I ACGT 6 cut(s) 582, 1117, 2282, 2837, 3713, 4001
KpnI GGTACC 1 cut(s) 1798
Ksp22I TGATCA 2 cut(s) 2259, 3735
KspAI GTTAAC 1 cut(s) 1050
Kzo9I GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
LmnI GCTCC 8 cut(s) 193, 890, 950, 1262, 1531, 2863, 3452, 3693
Lsp1109I GCAGC 8 cut(s) 30, 298, 1078, 1462, 2671, 3585, 3697, 4002
MaeII ACGT 6 cut(s) 582, 1117, 2282, 2837, 3713, 4001
MalI GATC 9 cut(s) 210, 323, 390, 569, 1535, 1803, 2261, 3632, 3737
MboI GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
MfeI CAATTG 5 cut(s) 591, 1770, 2713, 3307, 3552
MflI RGATCY 3 cut(s) 321, 1533, 3630
MhlI GDGCHC 7 cut(s) 895, 1528, 1731, 2067, 2733, 2928, 3241
MlsI TGGCCA 2 cut(s) 3225, 3913
MluNI TGGCCA 2 cut(s) 3225, 3913
MlyI GAGTC 8 cut(s) 970, 1724, 2051, 2790, 3287, 3702, 3950, 4034
MmeI TCCRAC 6 cut(s) 487, 1435, 1561, 2457, 2658, 2926
Mox20I TGGCCA 2 cut(s) 3225, 3913
Mph1103I ATGCAT 3 cut(s) 1446, 2173, 2657
MroXI GAANNNNTTC 1 cut(s) 3165
MscI TGGCCA 2 cut(s) 3225, 3913
MslI CAYNNNNRTG 4 cut(s) 263, 458, 2875, 3264
Msp20I TGGCCA 2 cut(s) 3225, 3913
MspA1I CMGCKG 3 cut(s) 1993, 2662, 3993
MspCI CTTAAG 1 cut(s) 3473
MspI CCGG 2 cut(s) 3359, 3825
MspR9I CCNGG 3 cut(s) 425, 606, 3869
MunI CAATTG 5 cut(s) 591, 1770, 2713, 3307, 3552
Mva1269I GAATGC 4 cut(s) 1188, 1977, 2657, 3809
MvaI CCWGG 3 cut(s) 425, 606, 3869
MvnI CGCG 1 cut(s) 922
MwoI GCNNNNNNNGC 4 cut(s) 472, 1472, 2037, 2138
NdeI CATATG 2 cut(s) 484, 1951
NdeII GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
NheI GCTAGC 1 cut(s) 3805
NlaIV GGNNCC 7 cut(s) 1535, 1796, 2065, 2436, 2522, 3454, 3632
NmuCI GTSAC 7 cut(s) 709, 733, 845, 913, 1367, 1398, 2913
NsiI ATGCAT 3 cut(s) 1446, 2173, 2657
NspI RCATGY 5 cut(s) 638, 846, 1019, 2227, 2659
PaeI GCATGC 1 cut(s) 2659
PagI TCATGA 4 cut(s) 556, 2256, 2814, 2920
PciI ACATGT 4 cut(s) 634, 842, 1015, 2223
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PctI GAATGC 4 cut(s) 1188, 1977, 2657, 3809
PdmI GAANNNNTTC 1 cut(s) 3165
PfeI GAWTC 9 cut(s) 609, 1766, 1835, 2074, 2644, 3001, 3070, 3161, 3673
PflFI GACNNNGTC 3 cut(s) 71, 979, 2089
PflMI CCANNNNNTGG 1 cut(s) 672
PkrI GCNGC 9 cut(s) 28, 45, 288, 1093, 1477, 2661, 3600, 3687, 3992
PleI GAGTC 8 cut(s) 970, 1723, 2050, 2790, 3286, 3702, 3950, 4034
PpsI GAGTC 8 cut(s) 970, 1723, 2050, 2790, 3286, 3702, 3950, 4034
Ppu21I YACGTR 3 cut(s) 583, 2838, 4002
PpuMI RGGWCCY 1 cut(s) 2435
PscI ACATGT 4 cut(s) 634, 842, 1015, 2223
PsiI TTATAA 2 cut(s) 726, 2685
Psp124BI GAGCTC 1 cut(s) 2928
Psp5II RGGWCCY 1 cut(s) 2435
Psp6I CCWGG 3 cut(s) 423, 604, 3867
PspEI GGTNACC 1 cut(s) 2607
PspGI CCWGG 3 cut(s) 423, 604, 3867
PspN4I GGNNCC 7 cut(s) 1535, 1796, 2065, 2436, 2522, 3454, 3632
PspOMI GGGCCC 1 cut(s) 2063
PspPI GGNCC 8 cut(s) 450, 497, 837, 1643, 1652, 2063, 2064, 2435
PspPPI RGGWCCY 1 cut(s) 2435
PstI CTGCAG 4 cut(s) 649, 1503, 2109, 3687
PstNI CAGNNNCTG 2 cut(s) 43, 3993
PsuI RGATCY 3 cut(s) 321, 1533, 3630
PsyI GACNNNGTC 3 cut(s) 71, 979, 2089
PvuII CAGCTG 3 cut(s) 1993, 2662, 3993
RsaI GTAC 9 cut(s) 315, 1348, 1704, 1796, 1956, 2402, 2898, 3176, 3231
RsaNI GTAC 9 cut(s) 314, 1347, 1703, 1795, 1955, 2401, 2897, 3175, 3230
RseI CAYNNNNRTG 4 cut(s) 263, 458, 2875, 3264
SacI GAGCTC 1 cut(s) 2928
SatI GCNGC 9 cut(s) 27, 44, 287, 1092, 1476, 2660, 3599, 3686, 3991
Sau3AI GATC 9 cut(s) 208, 321, 388, 567, 1533, 1801, 2259, 3630, 3735
Sau96I GGNCC 8 cut(s) 450, 497, 837, 1643, 1652, 2063, 2064, 2435
ScaI AGTACT 2 cut(s) 2898, 3176
SchI GAGTC 8 cut(s) 970, 1724, 2051, 2790, 3287, 3702, 3950, 4034
ScrFI CCNGG 3 cut(s) 425, 606, 3869
SduI GDGCHC 7 cut(s) 895, 1528, 1731, 2067, 2733, 2928, 3241
SfcI CTRYAG 6 cut(s) 501, 645, 954, 1499, 2105, 3683
SinI GGWCC 6 cut(s) 450, 497, 837, 1643, 1652, 2435
SmiMI CAYNNNNRTG 4 cut(s) 263, 458, 2875, 3264
SmlI CTYRAG 8 cut(s) 212, 701, 2581, 2759, 3049, 3170, 3340, 3473
SmoI CTYRAG 8 cut(s) 212, 701, 2581, 2759, 3049, 3170, 3340, 3473
SnaBI TACGTA 1 cut(s) 2838
SphI GCATGC 1 cut(s) 2659
SsiI CCGC 3 cut(s) 26, 922, 1612
SspI AATATT 1 cut(s) 3921
SstI GAGCTC 1 cut(s) 2928
StyD4I CCNGG 3 cut(s) 423, 604, 3867
StyI CCWWGG 5 cut(s) 446, 666, 1529, 3233, 3634
TaiI ACGT 6 cut(s) 585, 1120, 2285, 2840, 3716, 4004
TaqII GACCGA 1 cut(s) 1386
TatI WGTACW 4 cut(s) 1346, 2400, 2896, 3174
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 9 cut(s) 609, 1766, 1835, 2074, 2644, 3001, 3070, 3161, 3673
TscAI CASTG 6 cut(s) 195, 649, 2016, 2478, 2730, 3014
TseFI GTSAC 7 cut(s) 709, 733, 845, 913, 1367, 1398, 2913
TseI GCWGC 8 cut(s) 43, 286, 1091, 1475, 2659, 3598, 3685, 3990
Tsp45I GTSAC 7 cut(s) 709, 733, 845, 913, 1367, 1398, 2913
TspGWI ACGGA 1 cut(s) 3323
TspRI CASTG 6 cut(s) 195, 649, 2016, 2478, 2730, 3014
Tth111I GACNNNGTC 3 cut(s) 71, 979, 2089
Van91I CCANNNNNTGG 1 cut(s) 672
Vha464I CTTAAG 1 cut(s) 3473
VneI GTGCAC 1 cut(s) 2729
VpaK11BI GGWCC 6 cut(s) 450, 497, 837, 1643, 1652, 2435
XbaI TCTAGA 1 cut(s) 823
XceI RCATGY 5 cut(s) 638, 846, 1019, 2227, 2659
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 3165
ZrmI AGTACT 2 cut(s) 2898, 3176
Zsp2I ATGCAT 3 cut(s) 1446, 2173, 2657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.