Rorug01G0095700

tRNA uridine 5-carboxymethylaminomethyl modification enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007752
Physical Location & Seq
Reverse (-)
15836532 .. 15838946
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0095700.1

Sequence Viewer

Length: 960 bp
ATGGCTGATCCCCTGGTTGCTAGGTGCCTTTACTCTGGCAAAGGTTATATGATTCCTTTGAATCAATGCATGAGCTACTCTGAGTTGTATGAAGACATTTTCCGTACATTCCAGTTTTTGCCAAGTGATATTATTGAGCTTCAGTATGCAGTTCCAGGTTGTGAAGTTTGTTTTCTTCGTAACGATCGTGATTTCCAGATGCTGTTTTGCTCTGCTAGAATACATAGAGCAGTTGGTGGTGCCGATATTATTGACCCTTTTCTTGCTGAATTGCCTGTGGAAAATTGGTGCCGTGCATTTTATACTAGCTGTCGATATGGAATTATGGCTAATAGGATTGCTGAATCATTTAACTCTTGGATTGCAATTGAGCGTTTGATGCCAGTCTATTGTATGCTGGACCAGACAAGAATAAAACAAATGGAGATGGCGGGTAAGAGGAGGGAAGAGGCAGAACGTTGGACCACAGAACTAACTCCCAAAATGGAGGAAAGGTTGAAGGTGCAGATGGAGAAATCTCGTTGTTTCAGTGTCCATTATTCTAGCCTTGGAGTTTATGAGGTTAGATCTGACTTTTCATATGTAGTCAACATTTCCGATCATTCATGTTCATGTGTGAAATGGCAGATCAATTGTTTTCCTTGTCCTCACGGCCTTGCTGCATTACAAGCTGCTTCTGAGAATGTATATGATTATATTGATAAGTACTTTCATGTTGATATGTTCAAGAAGAGCTACAGTTTTCCCATCCGTCCGATAACCAATGTTGATATGTCTTCTTCGGAATCTGCTTCTGAATGTATATTACCTCCCCTTGCGAAGAGGCCCCCCGGGAGGCCTAGGGTGAAGCGGTTCAAGTCGGTTGGAGAGGTTGAAAAGAAGCTGATTCGTTGTGGTCGTTGTGGCAAAATGGGCACTCATAACAAGTTGAGCTGCACTGAACCTCTCGTTCATCAGTAG

Protein Analysis

319

Amino Acids

36.83

Weight (kDa)

8.24

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 194 - 225 3.1e-06 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 24, 239, 288
AciI CCGC 2 cut(s) 431, 850
AclI AACGTT 1 cut(s) 457
AclWI GGATC 1 cut(s) 2
AcuI CTGAAG 1 cut(s) 125
AfaI GTAC 2 cut(s) 106, 707
AfiI CCNNNNNNNGG 1 cut(s) 834
AgsI TTSAA 5 cut(s) 61, 499, 727, 856, 875
AjnI CCWGG 2 cut(s) 12, 154
AluBI AGCT 7 cut(s) 75, 139, 309, 671, 735, 883, 933
AluI AGCT 7 cut(s) 75, 139, 309, 671, 735, 883, 933
AlwI GGATC 1 cut(s) 2
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 830
AoxI GGCC 3 cut(s) 652, 824, 836
ApeKI GCWGC 3 cut(s) 659, 671, 933
Asp700I GAANNNNTTC 1 cut(s) 851
AspA2I CCTAGG 1 cut(s) 839
AspS9I GGNCC 3 cut(s) 400, 462, 825
AsuC2I CCSGG 2 cut(s) 831, 832
AsuHPI GGTGA 1 cut(s) 856
AvaI CYCGRG 1 cut(s) 830
AvaII GGWCC 2 cut(s) 400, 462
AvrII CCTAGG 1 cut(s) 839
BaeGI GKGCMC 1 cut(s) 917
BanI GGYRCC 3 cut(s) 24, 239, 288
BbsI GAAGAC 2 cut(s) 99, 768
BbvI GCAGC 3 cut(s) 646, 658, 920
BccI CCATC 3 cut(s) 421, 502, 755
BceAI ACGGC 2 cut(s) 276, 667
BciT130I CCWGG 2 cut(s) 14, 156
BcnI CCSGG 2 cut(s) 831, 832
BfaI CTAG 5 cut(s) 21, 216, 306, 543, 840
BfmI CTRYAG 1 cut(s) 736
BglII AGATCT 1 cut(s) 566
BisI GCNGC 3 cut(s) 660, 672, 934
BlnI CCTAGG 1 cut(s) 839
BlsI GCNGC 3 cut(s) 661, 673, 935
BmcAI AGTACT 1 cut(s) 707
Bme1390I CCNGG 4 cut(s) 14, 156, 831, 832
Bme18I GGWCC 2 cut(s) 400, 462
BmeT110I CYCGRG 1 cut(s) 830
BmgT120I GGNCC 3 cut(s) 400, 462, 825
BmiI GGNNCC 4 cut(s) 26, 241, 290, 827
BmrFI CCNGG 4 cut(s) 14, 156, 831, 832
BmsI GCATC 2 cut(s) 189, 369
BpiI GAAGAC 2 cut(s) 99, 768
BpuMI CCSGG 2 cut(s) 831, 832
BsaJI CCNNGG 5 cut(s) 12, 547, 829, 830, 839
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 834
Bse1I ACTGG 2 cut(s) 112, 383
BseBI CCWGG 2 cut(s) 14, 156
BseDI CCNNGG 5 cut(s) 12, 547, 829, 830, 839
BseGI GGATG 1 cut(s) 747
BseLI CCNNNNNNNGG 1 cut(s) 834
BseMII CTCAG 2 cut(s) 72, 669
BseNI ACTGG 2 cut(s) 112, 383
BseRI GAGGAG 1 cut(s) 454
BseSI GKGCMC 1 cut(s) 917
BseXI GCAGC 3 cut(s) 646, 658, 920
BsgI GTGCAG 2 cut(s) 524, 919
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 3 cut(s) 654, 826, 838
BshNI GGYRCC 3 cut(s) 24, 239, 288
BsiEI CGRYCG 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 1 cut(s) 831
BslI CCNNNNNNNGG 1 cut(s) 834
BsnI GGCC 3 cut(s) 654, 826, 838
BsoBI CYCGRG 1 cut(s) 830
Bsp1286I GDGCHC 1 cut(s) 917
Bsp143I GATC 5 cut(s) 7, 184, 566, 598, 627
BspACI CCGC 2 cut(s) 431, 850
BspANI GGCC 3 cut(s) 654, 826, 838
BspCNI CTCAG 2 cut(s) 73, 670
BspLI GGNNCC 4 cut(s) 26, 241, 290, 827
BspPI GGATC 1 cut(s) 2
BspQI GCTCTTC 1 cut(s) 725
BspT107I GGYRCC 3 cut(s) 24, 239, 288
BsrI ACTGG 2 cut(s) 112, 383
BssECI CCNNGG 5 cut(s) 12, 547, 829, 830, 839
BssMI GATC 5 cut(s) 7, 184, 566, 598, 627
BssT1I CCWWGG 2 cut(s) 547, 839
Bst2UI CCWGG 2 cut(s) 14, 156
Bst4CI ACNGT 1 cut(s) 740
Bst6I CTCTTC 3 cut(s) 441, 725, 815
BstDEI CTNAG 2 cut(s) 81, 678
BstF5I GGATG 1 cut(s) 747
BstKTI GATC 5 cut(s) 10, 187, 569, 601, 630
BstMBI GATC 5 cut(s) 7, 184, 566, 598, 627
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 379, 668, 912
BstNI CCWGG 2 cut(s) 14, 156
BstSCI CCNGG 4 cut(s) 12, 154, 829, 830
BstSFI CTRYAG 1 cut(s) 736
BstSLI GKGCMC 1 cut(s) 917
BstV1I GCAGC 3 cut(s) 646, 658, 920
BstV2I GAAGAC 2 cut(s) 99, 768
BstX2I RGATCY 1 cut(s) 566
BstYI RGATCY 1 cut(s) 566
BsuRI GGCC 3 cut(s) 654, 826, 838
BtsCI GGATG 1 cut(s) 747
BtsIMutI CAGTG 2 cut(s) 535, 936
CaiI CAGNNNCTG 1 cut(s) 202
Cfr13I GGNCC 3 cut(s) 400, 462, 825
Cfr9I CCCGGG 1 cut(s) 830
Csp6I GTAC 2 cut(s) 105, 706
CviAII CATG 4 cut(s) 70, 606, 612, 713
CviQI GTAC 2 cut(s) 105, 706
DdeI CTNAG 2 cut(s) 81, 678
DpnI GATC 5 cut(s) 9, 186, 568, 600, 629
DpnII GATC 5 cut(s) 7, 184, 566, 598, 627
Eam1104I CTCTTC 3 cut(s) 441, 725, 815
EarI CTCTTC 3 cut(s) 441, 725, 815
Eco130I CCWWGG 2 cut(s) 547, 839
Eco147I AGGCCT 1 cut(s) 838
Eco47I GGWCC 2 cut(s) 400, 462
Eco57I CTGAAG 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 830
EcoO109I RGGNCCY 1 cut(s) 825
EcoRII CCWGG 2 cut(s) 12, 154
EcoT14I CCWWGG 2 cut(s) 547, 839
EcoT22I ATGCAT 1 cut(s) 71
ErhI CCWWGG 2 cut(s) 547, 839
FaeI CATG 4 cut(s) 73, 609, 615, 716
FatI CATG 4 cut(s) 69, 605, 611, 712
FauI CCCGC 1 cut(s) 424
FauNDI CATATG 1 cut(s) 580
Fnu4HI GCNGC 3 cut(s) 660, 672, 934
FokI GGATG 1 cut(s) 734
Fsp4HI GCNGC 3 cut(s) 660, 672, 934
FspBI CTAG 5 cut(s) 21, 216, 306, 543, 840
GluI GCNGC 3 cut(s) 660, 672, 934
HaeIII GGCC 3 cut(s) 654, 826, 838
HapII CCGG 1 cut(s) 831
Hin1II CATG 4 cut(s) 73, 609, 615, 716
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HinfI GANTC 5 cut(s) 52, 61, 344, 785, 886
HpaII CCGG 1 cut(s) 831
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 7 cut(s) 82, 571, 598, 679, 756, 784, 796
Hpy188III TCNNGA 3 cut(s) 188, 196, 727
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 7 cut(s) 69, 149, 296, 365, 505, 662, 936
HpyF10VI GCNNNNNNNGC 3 cut(s) 379, 668, 912
HpyF3I CTNAG 2 cut(s) 81, 678
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 4 cut(s) 73, 609, 615, 716
Kzo9I GATC 5 cut(s) 7, 184, 566, 598, 627
LguI GCTCTTC 1 cut(s) 725
Lsp1109I GCAGC 3 cut(s) 646, 658, 920
LweI GCATC 2 cut(s) 189, 369
MaeI CTAG 5 cut(s) 21, 216, 306, 543, 840
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 1 cut(s) 179
MalI GATC 5 cut(s) 9, 186, 568, 600, 629
MboI GATC 5 cut(s) 7, 184, 566, 598, 627
MboII GAAGA 7 cut(s) 104, 167, 458, 742, 768, 771, 832
MfeI CAATTG 2 cut(s) 366, 631
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 1 cut(s) 917
MluCI AATT 5 cut(s) 269, 283, 321, 366, 631
MmeI TCCRAC 2 cut(s) 440, 844
Mph1103I ATGCAT 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 851
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 1 cut(s) 610
MspI CCGG 1 cut(s) 831
MspR9I CCNGG 4 cut(s) 14, 156, 831, 832
MunI CAATTG 2 cut(s) 366, 631
MvaI CCWGG 2 cut(s) 14, 156
MwoI GCNNNNNNNGC 3 cut(s) 379, 668, 912
NciI CCSGG 2 cut(s) 831, 832
NdeI CATATG 1 cut(s) 580
NdeII GATC 5 cut(s) 7, 184, 566, 598, 627
NlaIII CATG 4 cut(s) 73, 609, 615, 716
NlaIV GGNNCC 4 cut(s) 26, 241, 290, 827
NsiI ATGCAT 1 cut(s) 71
PceI AGGCCT 1 cut(s) 838
PciSI GCTCTTC 1 cut(s) 725
PcsI WCGNNNNNNNCGW 2 cut(s) 184, 895
PdmI GAANNNNTTC 1 cut(s) 851
PfeI GAWTC 5 cut(s) 52, 61, 344, 785, 886
PkrI GCNGC 3 cut(s) 661, 673, 935
Ple19I CGATCG 1 cut(s) 187
Psp1406I AACGTT 1 cut(s) 457
Psp6I CCWGG 2 cut(s) 12, 154
PspGI CCWGG 2 cut(s) 12, 154
PspN4I GGNNCC 4 cut(s) 26, 241, 290, 827
PspPI GGNCC 3 cut(s) 400, 462, 825
PstNI CAGNNNCTG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 566
PvuI CGATCG 1 cut(s) 187
RsaI GTAC 2 cut(s) 106, 707
RsaNI GTAC 2 cut(s) 105, 706
RseI CAYNNNNRTG 1 cut(s) 610
SapI GCTCTTC 1 cut(s) 725
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 660, 672, 934
Sau3AI GATC 5 cut(s) 7, 184, 566, 598, 627
Sau96I GGNCC 3 cut(s) 400, 462, 825
ScaI AGTACT 1 cut(s) 707
ScrFI CCNGG 4 cut(s) 14, 156, 831, 832
SduI GDGCHC 1 cut(s) 917
SfaNI GCATC 2 cut(s) 189, 369
SfcI CTRYAG 1 cut(s) 736
SinI GGWCC 2 cut(s) 400, 462
SmaI CCCGGG 1 cut(s) 832
SmiMI CAYNNNNRTG 1 cut(s) 610
Sse9I AATT 5 cut(s) 269, 283, 321, 366, 631
SseBI AGGCCT 1 cut(s) 838
SsiI CCGC 2 cut(s) 431, 850
SspMI CTAG 5 cut(s) 21, 216, 306, 543, 840
StuI AGGCCT 1 cut(s) 838
StyD4I CCNGG 4 cut(s) 12, 154, 829, 830
StyI CCWWGG 2 cut(s) 547, 839
TaaI ACNGT 1 cut(s) 740
TaiI ACGT 1 cut(s) 460
TaqI TCGA 1 cut(s) 313
TasI AATT 5 cut(s) 269, 283, 321, 366, 631
TatI WGTACW 1 cut(s) 705
TfiI GAWTC 5 cut(s) 52, 61, 344, 785, 886
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 2 cut(s) 535, 943
TseI GCWGC 3 cut(s) 659, 671, 933
TspDTI ATGAA 6 cut(s) 105, 567, 594, 600, 701, 941
TspGWI ACGGA 2 cut(s) 92, 740
TspMI CCCGGG 1 cut(s) 830
TspRI CASTG 2 cut(s) 535, 943
VpaK11BI GGWCC 2 cut(s) 400, 462
XmaI CCCGGG 1 cut(s) 830
XmaJI CCTAGG 1 cut(s) 839
XmnI GAANNNNTTC 1 cut(s) 851
XspI CTAG 5 cut(s) 21, 216, 306, 543, 840
ZrmI AGTACT 1 cut(s) 707
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.