Rorug01G0207000

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007234
Physical Location & Seq
Forward (+)
29172191 .. 29180352
8162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0207000.1

Sequence Viewer

Length: 1332 bp
ATGAGCAGGGCTTGCAGTCTCTTCCAACTATGGCACAGTCCTCCAGACACCAGATTACTACTCTCAATCACCAAAGGGCCATACTTGATACCTAGTGCCAGAAGTTATCCAGGTCTGTGCTTCTGTAAGGGTTTACAGACTTCTACATACGAAGTTGTCAAGGGAAGCTATGTACCCACCTCCCAGATTAAACAAGAAAGTAAAACTGAGGAAAAGTCTCCAGAAATACTTGAAGGTGTTGGCGCATTTCAAAAGCTACCAATGGTAATGCCATCCGTTGACATTCTGTATTCAGCACTAAGGAAGGCTAAGAAAATTGCACCAACAAAAGGCATTGCCAATATTGCAAAGAGAGAAAGAAATAGAGGTGCAAAGCAACTTGATGCATTGATGAAAGAAATAGCAGTTCCGTTGAGGACTTATTCGGAGAACTTTCCCAATAAGAAGTATCTGCACCCTTATGAGCGATCTCTTATTGAATTAACTCTCGGGGATGGAAATTATGAACAGGTGTTGGGAAAGGTGGATGCTCTGAGGAAAAAGGTTGTGTCTGTTGGAAAGGAACATGCATCTCTTTGTGCTAAGTCAACGTCGAAGAAGGAAGCAGAAACAAGACTGAGTGAGGGTTTAAAGAAAGTTGAGGAGACATATATTCGTCAAGGAAAATATGTTGATGATTTGTTACACATAGCCAAGACATTGCGAGCAATGCCGGTAGTTGATCTAGAAACACCAACACTTTGTCTTGTTGGAGCTCCTAATGTTGGGAAGTCATCTTTGGTTCGTATACTCTCCACAGGGAAGCCTGAGGTCTGCAACTACCCTTTCACAACAAGAGGAATTCTAATGGGTCATATCATTTTAAACCATGAGACTTTTCAGGTGACTGATACTCCGGGCCTGCTGAAGAGATGTGATGAGGACAGAAATAATTTGGAAAAATTGACACTTGCTGTCCTCTCACATCTGCCAACTGCAGTACTGTATGTCCATGATCTTTCTGGAGAATGTGGGACCTCACCTACTGATCAGTTTGTCATATACAATGAAATAAAGGAGAAATTTGGTGACCATCTGTGGCTTGATGTTGTGTCCAAATGCGATCTATTGCAAGAGTCTCCAGTGCTATTTGCTACTGATAATGACGATGATAATGATCTTGACCTGGCAAGGTACCGGAAATTGGGCCCTGAGGGAGCTCTTCATGTGTCAGTGAAAAATGAAGTAGGGCTCCCAGAGTTAAAAAGAAGAGTGCAAGAACTGCTGAATACCCAAATGACCAGGATCCAAAGTCTGGAAACCAACCAAGAAACTCTTCAAGTTCTTACCTGA

Protein Analysis

443

Amino Acids

49.52

Weight (kDa)

8.4

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 83 - 241 2.4e-48 NOG1 N-terminal helical domain
FeoB_N PF02421 246 - 303 5.3e-09 Ferrous iron transport protein B
MMR_HSR1 PF01926 247 - 343 4.5e-16 50S ribosome-binding GTPase
NOG1 PF06858 312 - 368 2.7e-17 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1173
AccB1I GGYRCC 1 cut(s) 1173
AccB7I CCANNNNNTGG 1 cut(s) 1294
AccI GTMKAC 1 cut(s) 787
AclWI GGATC 2 cut(s) 1279, 1292
AcsI RAATTY 2 cut(s) 840, 1061
AcuI CTGAAG 1 cut(s) 926
AfaI GTAC 3 cut(s) 174, 981, 1175
AfiI CCNNNNNNNGG 4 cut(s) 329, 764, 1183, 1294
AgsI TTSAA 4 cut(s) 233, 251, 479, 1319
AjnI CCWGG 3 cut(s) 109, 1164, 1280
AjuI GAANNNNNNNTTGG 4 cut(s) 761, 793, 1299, 1331
AluBI AGCT 4 cut(s) 168, 256, 755, 1199
AluI AGCT 4 cut(s) 168, 256, 755, 1199
Alw21I GWGCWC 2 cut(s) 757, 1201
Alw26I GTCTC 5 cut(s) 23, 222, 638, 866, 1122
AlwI GGATC 2 cut(s) 1279, 1292
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 3 cut(s) 77, 898, 1186
ApaI GGGCCC 1 cut(s) 1190
ApoI RAATTY 2 cut(s) 840, 1061
Asp700I GAANNNNTTC 1 cut(s) 1314
Asp718I GGTACC 1 cut(s) 1173
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 5 cut(s) 77, 898, 1014, 1186, 1187
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 4 cut(s) 61, 895, 1011, 1079
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 1014
AxyI CCTNAGG 2 cut(s) 807, 1191
BaeGI GKGCMC 1 cut(s) 1190
BamHI GGATCC 1 cut(s) 1284
BanI GGYRCC 1 cut(s) 1173
BanII GRGCYC 4 cut(s) 757, 1190, 1201, 1233
Bbv12I GWGCWC 2 cut(s) 757, 1201
BccI CCATC 3 cut(s) 280, 488, 1080
BciT130I CCWGG 3 cut(s) 111, 1166, 1282
BclI TGATCA 1 cut(s) 1027
BcnI CCSGG 1 cut(s) 897
BcoDI GTCTC 5 cut(s) 23, 222, 638, 866, 1122
BfaI CTAG 2 cut(s) 93, 725
BfmI CTRYAG 1 cut(s) 975
BmcAI AGTACT 1 cut(s) 981
Bme1390I CCNGG 4 cut(s) 111, 897, 1166, 1282
Bme18I GGWCC 1 cut(s) 1014
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 5 cut(s) 77, 898, 1014, 1186, 1187
BmiI GGNNCC 5 cut(s) 1015, 1175, 1188, 1232, 1286
BmrFI CCNGG 4 cut(s) 111, 897, 1166, 1282
BmsI GCATC 3 cut(s) 373, 517, 578
BpmI CTGGAG 4 cut(s) 27, 204, 1023, 1104
BpuMI CCSGG 1 cut(s) 897
BsaBI GATNNNNATC 1 cut(s) 1155
BsaWI WCCGGW 1 cut(s) 1176
BsaXI ACNNNNNCTCC 2 cut(s) 877, 907
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 764, 1183, 1294
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 1121
Bse21I CCTNAGG 2 cut(s) 807, 1191
Bse3DI GCAATG 3 cut(s) 333, 698, 714
Bse8I GATNNNNATC 1 cut(s) 1155
BseBI CCWGG 3 cut(s) 111, 1166, 1282
BseGI GGATG 3 cut(s) 272, 499, 532
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 4 cut(s) 329, 764, 1183, 1294
BseMI GCAATG 3 cut(s) 333, 698, 714
BseMII CTCAG 5 cut(s) 198, 524, 608, 798, 1182
BseNI ACTGG 1 cut(s) 1121
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 1 cut(s) 1190
BsgI GTGCAG 1 cut(s) 437
BshFI GGCC 3 cut(s) 79, 900, 1188
BshNI GGYRCC 1 cut(s) 1173
BsiHKAI GWGCWC 2 cut(s) 757, 1201
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 713, 896, 1177
BslFI GGGAC 1 cut(s) 1027
BslI CCNNNNNNNGG 4 cut(s) 329, 764, 1183, 1294
BsmAI GTCTC 5 cut(s) 23, 222, 638, 866, 1122
BsmFI GGGAC 1 cut(s) 1027
BsnI GGCC 3 cut(s) 79, 900, 1188
BsoBI CYCGRG 1 cut(s) 488
Bsp120I GGGCCC 1 cut(s) 1186
Bsp1286I GDGCHC 4 cut(s) 757, 1190, 1201, 1233
Bsp143I GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
BspANI GGCC 3 cut(s) 79, 900, 1188
BspCNI CTCAG 5 cut(s) 199, 525, 609, 799, 1183
BspLI GGNNCC 5 cut(s) 1015, 1175, 1188, 1232, 1286
BspMAI CTGCAG 1 cut(s) 979
BspPI GGATC 2 cut(s) 1279, 1292
BspQI GCTCTTC 1 cut(s) 1206
BspT107I GGYRCC 1 cut(s) 1173
BsrDI GCAATG 3 cut(s) 333, 698, 714
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 1 cut(s) 1121
BssAI RCCGGY 1 cut(s) 712
BssMI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
BssNAI GTATAC 1 cut(s) 788
Bst1107I GTATAC 1 cut(s) 788
Bst2UI CCWGG 3 cut(s) 111, 1166, 1282
Bst4CI ACNGT 2 cut(s) 38, 984
Bst6I CTCTTC 5 cut(s) 26, 902, 1206, 1243, 1320
BstAPI GCANNNNNTGC 2 cut(s) 12, 1261
BstC8I GCNNGC 3 cut(s) 13, 705, 902
BstDEI CTNAG 8 cut(s) 207, 299, 309, 533, 582, 617, 807, 1191
BstEII GGTNACC 1 cut(s) 1067
BstF5I GGATG 3 cut(s) 272, 499, 532
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 7 cut(s) 470, 724, 997, 1030, 1105, 1159, 1287
BstMAI GTCTC 5 cut(s) 23, 222, 638, 866, 1122
BstMBI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
BstMWI GCNNNNNNNGC 4 cut(s) 12, 344, 709, 1261
BstNI CCWGG 3 cut(s) 111, 1166, 1282
BstNSI RCATGY 1 cut(s) 569
BstPI GGTNACC 1 cut(s) 1067
BstSCI CCNGG 4 cut(s) 109, 895, 1164, 1280
BstSFI CTRYAG 1 cut(s) 975
BstSLI GKGCMC 1 cut(s) 1190
BstX2I RGATCY 1 cut(s) 1284
BstYI RGATCY 1 cut(s) 1284
BstZ17I GTATAC 1 cut(s) 788
Bsu36I CCTNAGG 2 cut(s) 807, 1191
BsuRI GGCC 3 cut(s) 79, 900, 1188
BtsCI GGATG 3 cut(s) 272, 499, 532
BtsIMutI CAGTG 2 cut(s) 1128, 1218
Cac8I GCNNGC 3 cut(s) 13, 705, 902
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 5 cut(s) 77, 898, 1014, 1186, 1187
Csp6I GTAC 3 cut(s) 173, 980, 1174
CviAII CATG 4 cut(s) 566, 869, 992, 1205
CviQI GTAC 3 cut(s) 173, 980, 1174
DdeI CTNAG 8 cut(s) 207, 299, 309, 533, 582, 617, 807, 1191
DpnI GATC 7 cut(s) 469, 723, 996, 1029, 1104, 1158, 1286
DpnII GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
DraI TTTAAA 2 cut(s) 630, 864
Eam1104I CTCTTC 5 cut(s) 26, 902, 1206, 1243, 1320
EarI CTCTTC 5 cut(s) 26, 902, 1206, 1243, 1320
Ecl136II GAGCTC 2 cut(s) 755, 1199
Eco24I GRGCYC 4 cut(s) 757, 1190, 1201, 1233
Eco47I GGWCC 1 cut(s) 1014
Eco53kI GAGCTC 2 cut(s) 755, 1199
Eco57I CTGAAG 1 cut(s) 926
Eco81I CCTNAGG 2 cut(s) 807, 1191
Eco88I CYCGRG 1 cut(s) 488
Eco91I GGTNACC 1 cut(s) 1067
EcoICRI GAGCTC 2 cut(s) 755, 1199
EcoO109I RGGNCCY 2 cut(s) 1014, 1187
EcoO65I GGTNACC 1 cut(s) 1067
EcoRI GAATTC 1 cut(s) 840
EcoRII CCWGG 3 cut(s) 109, 1164, 1280
EcoT22I ATGCAT 2 cut(s) 388, 571
EcoT38I GRGCYC 4 cut(s) 757, 1190, 1201, 1233
FaeI CATG 4 cut(s) 569, 872, 995, 1208
FalI AAGNNNNNCTT 2 cut(s) 1299, 1331
FaqI GGGAC 1 cut(s) 1027
FatI CATG 4 cut(s) 565, 868, 991, 1204
FbaI TGATCA 1 cut(s) 1027
FblI GTMKAC 1 cut(s) 787
FokI GGATG 3 cut(s) 259, 506, 539
FriOI GRGCYC 4 cut(s) 757, 1190, 1201, 1233
FspBI CTAG 2 cut(s) 93, 725
GlaI GCGC 1 cut(s) 244
GsuI CTGGAG 4 cut(s) 27, 204, 1023, 1104
HaeIII GGCC 3 cut(s) 79, 900, 1188
HapII CCGG 3 cut(s) 713, 896, 1177
HhaI GCGC 1 cut(s) 245
Hin1II CATG 4 cut(s) 569, 872, 995, 1208
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 2 cut(s) 280, 588
HindII GTYRAC 2 cut(s) 280, 588
HinfI GANTC 1 cut(s) 1115
HpaII CCGG 3 cut(s) 713, 896, 1177
HphI GGTGA 4 cut(s) 61, 895, 1011, 1079
Hpy166II GTNNAC 4 cut(s) 134, 280, 588, 788
Hpy188I TCNGA 2 cut(s) 427, 534
Hpy188III TCNNGA 6 cut(s) 44, 221, 725, 1002, 1160, 1295
Hpy8I GTNNAC 4 cut(s) 134, 280, 588, 788
Hpy99I CGWCG 1 cut(s) 595
HpyAV CCTTC 3 cut(s) 227, 298, 592
HpyCH4III ACNGT 2 cut(s) 38, 984
HpyCH4IV ACGT 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 344, 709, 1261
HpyF3I CTNAG 8 cut(s) 207, 299, 309, 533, 582, 617, 807, 1191
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 4 cut(s) 569, 872, 995, 1208
HspAI GCGC 1 cut(s) 243
KpnI GGTACC 1 cut(s) 1177
Ksp22I TGATCA 1 cut(s) 1027
Kzo9I GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 4 cut(s) 752, 760, 1196, 1236
LweI GCATC 3 cut(s) 373, 517, 578
MaeI CTAG 2 cut(s) 93, 725
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 3 cut(s) 681, 883, 1067
MalI GATC 7 cut(s) 469, 723, 996, 1029, 1104, 1158, 1286
MboI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
MboII GAAGA 6 cut(s) 13, 607, 919, 1193, 1260, 1307
MflI RGATCY 1 cut(s) 1284
MhlI GDGCHC 4 cut(s) 757, 1190, 1201, 1233
MluCI AATT 8 cut(s) 315, 479, 499, 840, 931, 941, 1061, 1181
MlyI GAGTC 1 cut(s) 1124
MmeI TCCRAC 3 cut(s) 49, 535, 730
Mph1103I ATGCAT 2 cut(s) 388, 571
MroXI GAANNNNTTC 1 cut(s) 1314
MseI TTAA 5 cut(s) 189, 482, 629, 863, 1241
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 3 cut(s) 713, 896, 1177
MspR9I CCNGG 4 cut(s) 111, 897, 1166, 1282
MvaI CCWGG 3 cut(s) 111, 1166, 1282
MwoI GCNNNNNNNGC 4 cut(s) 12, 344, 709, 1261
NciI CCSGG 1 cut(s) 897
NdeII GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
NlaIII CATG 4 cut(s) 569, 872, 995, 1208
NlaIV GGNNCC 5 cut(s) 1015, 1175, 1188, 1232, 1286
NmuCI GTSAC 2 cut(s) 883, 1067
NsiI ATGCAT 2 cut(s) 388, 571
NspI RCATGY 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 1206
PdmI GAANNNNTTC 1 cut(s) 1314
PflMI CCANNNNNTGG 1 cut(s) 1294
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
PpuMI RGGWCCY 1 cut(s) 1014
Psp124BI GAGCTC 2 cut(s) 757, 1201
Psp5II RGGWCCY 1 cut(s) 1014
Psp6I CCWGG 3 cut(s) 109, 1164, 1280
PspEI GGTNACC 1 cut(s) 1067
PspGI CCWGG 3 cut(s) 109, 1164, 1280
PspN4I GGNNCC 5 cut(s) 1015, 1175, 1188, 1232, 1286
PspOMI GGGCCC 1 cut(s) 1186
PspPI GGNCC 5 cut(s) 77, 898, 1014, 1186, 1187
PspPPI RGGWCCY 1 cut(s) 1014
PstI CTGCAG 1 cut(s) 979
PsuI RGATCY 1 cut(s) 1284
RsaI GTAC 3 cut(s) 174, 981, 1175
RsaNI GTAC 3 cut(s) 173, 980, 1174
RseI CAYNNNNRTG 1 cut(s) 459
SacI GAGCTC 2 cut(s) 757, 1201
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 5 cut(s) 189, 482, 629, 863, 1241
Sau3AI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
Sau96I GGNCC 5 cut(s) 77, 898, 1014, 1186, 1187
ScaI AGTACT 1 cut(s) 981
SchI GAGTC 1 cut(s) 1124
ScrFI CCNGG 4 cut(s) 111, 897, 1166, 1282
SduI GDGCHC 4 cut(s) 757, 1190, 1201, 1233
SfaNI GCATC 3 cut(s) 373, 517, 578
SfcI CTRYAG 1 cut(s) 975
SinI GGWCC 1 cut(s) 1014
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 8 cut(s) 315, 479, 499, 840, 931, 941, 1061, 1181
SspI AATATT 1 cut(s) 343
SspMI CTAG 2 cut(s) 93, 725
SstI GAGCTC 2 cut(s) 757, 1201
StyD4I CCNGG 4 cut(s) 109, 895, 1164, 1280
TaaI ACNGT 2 cut(s) 38, 984
TaiI ACGT 1 cut(s) 593
TaqI TCGA 1 cut(s) 593
TasI AATT 8 cut(s) 315, 479, 499, 840, 931, 941, 1061, 1181
TatI WGTACW 1 cut(s) 979
Tru1I TTAA 5 cut(s) 189, 482, 629, 863, 1241
Tru9I TTAA 5 cut(s) 189, 482, 629, 863, 1241
TscAI CASTG 2 cut(s) 1128, 1218
TseFI GTSAC 2 cut(s) 883, 1067
Tsp45I GTSAC 2 cut(s) 883, 1067
TspDTI ATGAA 5 cut(s) 407, 519, 1062, 1193, 1236
TspGWI ACGGA 2 cut(s) 265, 399
TspRI CASTG 2 cut(s) 1128, 1218
Van91I CCANNNNNTGG 1 cut(s) 1294
VpaK11BI GGWCC 1 cut(s) 1014
XapI RAATTY 2 cut(s) 840, 1061
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 787
XmnI GAANNNNTTC 1 cut(s) 1314
XspI CTAG 2 cut(s) 93, 725
ZrmI AGTACT 1 cut(s) 981
Zsp2I ATGCAT 2 cut(s) 388, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.