Rorug01G0291300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0016065
Physical Location & Seq
Forward (+)
40744628 .. 40747223
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0291300.1

Sequence Viewer

Length: 1464 bp
ATGGGATTGTTGAGAAGAAATCCCATGAAAACTCAAGTGATGAAATCAGTGTTAATAGTTGTTGTGGTGGCCTTAATACTAAGAGGGGGAGAGAGCAGAGGAGTAGCCAAGGCCAAGCCTACGGCCCCCATAAAATCTGAGTCCTTTGAGTACTCTGCTATGAATTGCAGAGCTCACAGTGCTTCACTGACCGAGTTTGGAGGAGTTGGAGATGGAAAGACATCAAATACAAAGGCTTTTCAGACTGCAATCAGTAAGCTGAGCCAGTTTGCAACAGATGGAGGTGCTCAGCTTTTTGTTCCAGCTGGGAAATGGTTGACAGGGAGCTTCAGCCTCACCAGCCATTTCACTCTTTATCTTCACAAGGATGCTGTTCTTCTTGCTTCTCAGGACTTGAATGAGTGGCCTGTGCTTAAGGCCCTTCCATCTTATGGCAGAGGAAGAGATGCAGCAGCTGGAAGGTACTCCAGTCTCATATTTGGAACAAATCTCACTGATGTCATTGTTACAGGAGACAATGGCACAATTGATGGTCAAGGTGAGTTCTGGTGGCAACAGTTCCACAAGAAGAAGCTAAAATACACCCGCCCTTATCTGATTGAGATCATGTTCTCAAGTGATATCCAGATCTCAGATCTCACCCTCCTAAATTCTCCATCATGGAATGTTCATCCTGTCTATAGCAGTAACATTCTTGTGCAAGGGATTACTATCATTGCTCCAATTTCGTCTCCAAACACTGATGGGATCAATCCAGATTCTTGCACAAATACTAGAATTGAAGACTGTTACATAGTCTCTGGTGATGATTGTGTTGCTGTTAAGAGCGGTTGGGATGAGTATGGCATATCATTTGGAATGCCTACAAAACAACTAGTGATCAGACGGCTCACATGCATTTCACCCTACAGTGCAACCATCGCTTTGGGAAGCGAAATGTCAGGTGGGATCCAAGACGTCAGAGCTGAAGACATCGTGGCCATCAATACCGAATCAGCCGTCAGGATCAAGACAGCTGTAGGGAGAGGAGGGTATGTGAAAGACATATATGTAAGGAGAATGACACTGCACACAATGAAATGGGCATTTCGGATGAATGGAAATTATGGGTCACATGCTGATGGTAACTATGACAAAAATGCCTTTCCTGAGATCACTGGGATCAACTATAGAGATGTGGTGGCTGAAAATGTAACCATCGCTGCAAGGTTGGAGGGCATTGCTGACCATCCATTTACTGGGATTTGCATGTCCAATGTGACCATTGGATTGGCAAAGAAGGCAAAGAAGCAGCCATGGACATGTACTGATATTCAGGGGATCACAAGCGGTGTGACGCCAACGCCCTGTGGTCTGCTGCCTGATCAAGGGACTGCTAAACCTGGTGGTTGTGATTTCCCAGCAGATGATATACCGATCGACATGGTAGAGATTAAAAAGTGTACTTATAGGAGTAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

52.85

Weight (kDa)

8.4

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 156 - 439 6.8e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016065)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41610
fragaria_vesca FvH4_7g18090 FvH4_7g18120
prunus_persica Prupe.2G203300_v2.0.a1
pyrus_communis pycom01g11750
rosa_chinensis RchiOBHm_Chr1g0361741
rosa_laevigata RLG00000027706
rosa_multiflora Rmu_sc0005733.1_g000002
rosa_roxburghii Rroxscaffold_4G00294730
rosa_rugosa Rorug01G0291300
rosa_samantha Rh1AG301700 Rh1BG264400 Rh1CG283000 Rh1DG294400
rosa_wichuraiana Rw1G026730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 960
AccB7I CCANNNNNTGG 2 cut(s) 431, 1238
AccBSI CCGCTC 1 cut(s) 828
AciI CCGC 3 cut(s) 586, 828, 1329
AclWI GGATC 6 cut(s) 755, 943, 956, 1013, 1169, 1328
AcoI YGGCCR 1 cut(s) 978
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 2 cut(s) 313, 987
AcyI GRCGYC 2 cut(s) 957, 1337
AfaI GTAC 4 cut(s) 152, 464, 1306, 1444
AfiI CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 1301
AgsI TTSAA 2 cut(s) 397, 782
AhlI ACTAGT 1 cut(s) 874
AjnI CCWGG 1 cut(s) 1381
AluBI AGCT 9 cut(s) 173, 259, 292, 305, 327, 455, 574, 965, 1016
AluI AGCT 9 cut(s) 173, 259, 292, 305, 327, 455, 574, 965, 1016
Alw21I GWGCWC 2 cut(s) 175, 289
Alw26I GTCTC 4 cut(s) 476, 507, 735, 802
AlwI GGATC 6 cut(s) 755, 943, 956, 1013, 1169, 1328
AlwNI CAGNNNCTG 1 cut(s) 455
AoxI GGCC 6 cut(s) 69, 111, 123, 404, 417, 978
ApeKI GCWGC 5 cut(s) 449, 452, 1202, 1291, 1357
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 2 cut(s) 984, 1016
AspS9I GGNCC 2 cut(s) 124, 418
AsuHPI GGTGA 5 cut(s) 328, 551, 631, 815, 894
BaeI ACNNNNGTAYC 2 cut(s) 454, 487
BalI TGGCCA 1 cut(s) 980
BamHI GGATCC 1 cut(s) 948
BanII GRGCYC 1 cut(s) 175
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 2 cut(s) 789, 975
Bbv12I GWGCWC 2 cut(s) 175, 289
BbvI GCAGC 5 cut(s) 461, 464, 1189, 1303, 1344
BceAI ACGGC 3 cut(s) 138, 902, 983
BciT130I CCWGG 1 cut(s) 1383
BclI TGATCA 2 cut(s) 879, 1363
BcoDI GTCTC 4 cut(s) 476, 507, 735, 802
BcuI ACTAGT 1 cut(s) 874
BfaI CTAG 2 cut(s) 774, 875
BfmI CTRYAG 4 cut(s) 679, 907, 1017, 1168
BfrI CTTAAG 1 cut(s) 413
BglII AGATCT 2 cut(s) 627, 634
BisI GCNGC 5 cut(s) 450, 453, 1203, 1292, 1358
BlpI GCTNAGC 2 cut(s) 260, 288
BlsI GCNGC 5 cut(s) 451, 454, 1204, 1293, 1359
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 1383
BmgT120I GGNCC 2 cut(s) 124, 418
BmiI GGNNCC 2 cut(s) 126, 950
BmrFI CCNGG 1 cut(s) 1383
BmrI ACTGGG 2 cut(s) 1167, 1248
BmsI GCATC 2 cut(s) 358, 436
BmuI ACTGGG 2 cut(s) 1167, 1248
BpiI GAAGAC 2 cut(s) 789, 975
BpmI CTGGAG 1 cut(s) 451
Bpu1102I GCTNAGC 2 cut(s) 260, 288
BpuEI CTTGAG 2 cut(s) 18, 598
BsaBI GATNNNNATC 2 cut(s) 602, 710
BsaHI GRCGYC 2 cut(s) 957, 1337
BsaJI CCNNGG 2 cut(s) 108, 1295
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
Bse1I ACTGG 4 cut(s) 265, 468, 1162, 1243
Bse3DI GCAATG 2 cut(s) 714, 1218
Bse8I GATNNNNATC 2 cut(s) 602, 710
BseBI CCWGG 1 cut(s) 1383
BseDI CCNNGG 2 cut(s) 108, 1295
BseGI GGATG 5 cut(s) 373, 670, 841, 1098, 1228
BseJI GATNNNNATC 2 cut(s) 602, 710
BseLI CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
BseMI GCAATG 2 cut(s) 714, 1218
BseMII CTCAG 6 cut(s) 129, 251, 302, 401, 645, 1140
BseNI ACTGG 4 cut(s) 265, 468, 1162, 1243
BseRI GAGGAG 3 cut(s) 114, 216, 1041
BseXI GCAGC 5 cut(s) 461, 464, 1189, 1303, 1344
BseYI CCCAGC 2 cut(s) 305, 1399
BsgI GTGCAG 1 cut(s) 1052
Bsh1285I CGRYCG 1 cut(s) 1419
BshFI GGCC 6 cut(s) 71, 113, 125, 406, 419, 980
BsiEI CGRYCG 1 cut(s) 1419
BsiHKAI GWGCWC 2 cut(s) 175, 289
BslFI GGGAC 1 cut(s) 1384
BslI CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
BsmAI GTCTC 4 cut(s) 476, 507, 735, 802
BsmBI CGTCTC 1 cut(s) 735
BsmFI GGGAC 1 cut(s) 1384
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 6 cut(s) 71, 113, 125, 406, 419, 980
Bsp1286I GDGCHC 2 cut(s) 175, 289
Bsp1720I GCTNAGC 2 cut(s) 260, 288
Bsp19I CCATGG 1 cut(s) 1295
BspACI CCGC 3 cut(s) 586, 828, 1329
BspANI GGCC 6 cut(s) 71, 113, 125, 406, 419, 980
BspCNI CTCAG 6 cut(s) 130, 252, 301, 400, 644, 1141
BspLI GGNNCC 2 cut(s) 126, 950
BspPI GGATC 6 cut(s) 755, 943, 956, 1013, 1169, 1328
BspTI CTTAAG 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 828
BsrDI GCAATG 2 cut(s) 714, 1218
BsrI ACTGG 4 cut(s) 265, 468, 1162, 1243
BssECI CCNNGG 2 cut(s) 108, 1295
BssNI GRCGYC 2 cut(s) 957, 1337
BssT1I CCWWGG 2 cut(s) 108, 1295
Bst2UI CCWGG 1 cut(s) 1383
Bst4CI ACNGT 4 cut(s) 179, 558, 788, 911
Bst6I CTCTTC 1 cut(s) 436
BstACI GRCGYC 2 cut(s) 957, 1337
BstAFI CTTAAG 1 cut(s) 413
BstDEI CTNAG 7 cut(s) 80, 138, 260, 288, 387, 631, 1149
BstDSI CCRYGG 1 cut(s) 1295
BstENI CCTNNNNNAGG 1 cut(s) 1365
BstF5I GGATG 5 cut(s) 373, 670, 841, 1098, 1228
BstMAI GTCTC 4 cut(s) 476, 507, 735, 802
BstMCI CGRYCG 1 cut(s) 1419
BstMWI GCNNNNNNNGC 4 cut(s) 179, 339, 920, 1280
BstNI CCWGG 1 cut(s) 1383
BstNSI RCATGY 4 cut(s) 897, 1118, 1252, 1305
BstSCI CCNGG 1 cut(s) 1381
BstSFI CTRYAG 4 cut(s) 679, 907, 1017, 1168
BstV1I GCAGC 5 cut(s) 461, 464, 1189, 1303, 1344
BstV2I GAAGAC 2 cut(s) 789, 975
BstX2I RGATCY 3 cut(s) 627, 634, 948
BstXI CCANNNNNNTGG 2 cut(s) 925, 1270
BstYI RGATCY 3 cut(s) 627, 634, 948
BsuRI GGCC 6 cut(s) 71, 113, 125, 406, 419, 980
BtgI CCRYGG 1 cut(s) 1295
BtgZI GCGATG 2 cut(s) 904, 1183
BtsCI GGATG 5 cut(s) 373, 670, 841, 1098, 1228
BtsI GCAGTG 1 cut(s) 1064
BtsIMutI CAGTG 8 cut(s) 54, 184, 185, 492, 738, 916, 1064, 1155
CaiI CAGNNNCTG 1 cut(s) 455
Cfr13I GGNCC 2 cut(s) 124, 418
CseI GACGC 1 cut(s) 1345
CsiI ACCWGGT 1 cut(s) 1381
Csp6I GTAC 4 cut(s) 151, 463, 1305, 1443
CviAII CATG 9 cut(s) 25, 607, 660, 894, 1115, 1249, 1296, 1302, 1423
CviQI GTAC 4 cut(s) 151, 463, 1305, 1443
DdeI CTNAG 7 cut(s) 80, 138, 260, 288, 387, 631, 1149
EaeI YGGCCR 1 cut(s) 978
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 108, 1295
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 313, 987
EcoICRI GAGCTC 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 1365
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 1381
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 2 cut(s) 108, 1295
EcoT22I ATGCAT 1 cut(s) 899
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 108, 1295
Esp3I CGTCTC 1 cut(s) 735
FaeI CATG 9 cut(s) 28, 610, 663, 897, 1118, 1252, 1299, 1305, 1426
FaqI GGGAC 1 cut(s) 1384
FatI CATG 9 cut(s) 24, 606, 659, 893, 1114, 1248, 1295, 1301, 1422
FauI CCCGC 1 cut(s) 593
FbaI TGATCA 2 cut(s) 879, 1363
Fnu4HI GCNGC 5 cut(s) 450, 453, 1203, 1292, 1358
FokI GGATG 5 cut(s) 380, 657, 848, 1105, 1215
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 5 cut(s) 450, 453, 1203, 1292, 1358
FspBI CTAG 2 cut(s) 774, 875
GluI GCNGC 5 cut(s) 450, 453, 1203, 1292, 1358
GsaI CCCAGC 2 cut(s) 309, 1403
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 6 cut(s) 71, 113, 125, 406, 419, 980
HgaI GACGC 1 cut(s) 1345
Hin1I GRCGYC 2 cut(s) 957, 1337
Hin1II CATG 9 cut(s) 28, 610, 663, 897, 1118, 1252, 1299, 1305, 1426
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HinfI GANTC 3 cut(s) 140, 758, 992
HphI GGTGA 5 cut(s) 328, 551, 631, 815, 894
Hpy166II GTNNAC 2 cut(s) 318, 1443
Hpy188I TCNGA 7 cut(s) 139, 243, 597, 634, 884, 962, 1092
Hpy188III TCNNGA 6 cut(s) 389, 625, 755, 1003, 1009, 1148
Hpy8I GTNNAC 2 cut(s) 318, 1443
HpyAV CCTTC 3 cut(s) 431, 453, 1273
HpyCH4III ACNGT 4 cut(s) 179, 558, 788, 911
HpyCH4IV ACGT 1 cut(s) 957
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 339, 920, 1280
HpyF3I CTNAG 7 cut(s) 80, 138, 260, 288, 387, 631, 1149
HpySE526I ACGT 1 cut(s) 957
Hsp92I GRCGYC 2 cut(s) 957, 1337
Hsp92II CATG 9 cut(s) 28, 610, 663, 897, 1118, 1252, 1299, 1305, 1426
Ksp22I TGATCA 2 cut(s) 879, 1363
LmnI GCTCC 2 cut(s) 324, 724
Lsp1109I GCAGC 5 cut(s) 461, 464, 1189, 1303, 1344
LweI GCATC 2 cut(s) 358, 436
MabI ACCWGGT 1 cut(s) 1381
MaeI CTAG 2 cut(s) 774, 875
MaeII ACGT 1 cut(s) 957
MaeIII GTNAC 8 cut(s) 505, 686, 788, 1110, 1124, 1192, 1258, 1333
MbiI CCGCTC 1 cut(s) 828
MboII GAAGA 7 cut(s) 27, 350, 368, 453, 580, 794, 980
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 3 cut(s) 627, 634, 948
MhlI GDGCHC 2 cut(s) 175, 289
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 6 cut(s) 163, 525, 649, 723, 777, 1102
MluNI TGGCCA 1 cut(s) 980
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 2 cut(s) 187, 1191
Mox20I TGGCCA 1 cut(s) 980
Mph1103I ATGCAT 1 cut(s) 899
MscI TGGCCA 1 cut(s) 980
MseI TTAA 5 cut(s) 53, 74, 414, 822, 1434
MslI CAYNNNNRTG 4 cut(s) 366, 695, 1119, 1300
Msp20I TGGCCA 1 cut(s) 980
MspA1I CMGCKG 3 cut(s) 305, 455, 1016
MspCI CTTAAG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 1383
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 1 cut(s) 1383
MwoI GCNNNNNNNGC 4 cut(s) 179, 339, 920, 1280
NcoI CCATGG 1 cut(s) 1295
NlaIII CATG 9 cut(s) 28, 610, 663, 897, 1118, 1252, 1299, 1305, 1426
NlaIV GGNNCC 2 cut(s) 126, 950
NmuCI GTSAC 3 cut(s) 1110, 1258, 1333
NsiI ATGCAT 1 cut(s) 899
NspI RCATGY 4 cut(s) 897, 1118, 1252, 1305
PciI ACATGT 1 cut(s) 1301
PctI GAATGC 1 cut(s) 864
PfeI GAWTC 2 cut(s) 758, 992
PflMI CCANNNNNTGG 2 cut(s) 431, 1238
PkrI GCNGC 5 cut(s) 451, 454, 1204, 1293, 1359
Ple19I CGATCG 1 cut(s) 1419
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PscI ACATGT 1 cut(s) 1301
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 1381
PspFI CCCAGC 2 cut(s) 305, 1399
PspGI CCWGG 1 cut(s) 1381
PspN4I GGNNCC 2 cut(s) 126, 950
PspPI GGNCC 2 cut(s) 124, 418
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 3 cut(s) 627, 634, 948
PvuI CGATCG 1 cut(s) 1419
PvuII CAGCTG 3 cut(s) 305, 455, 1016
RsaI GTAC 4 cut(s) 152, 464, 1306, 1444
RsaNI GTAC 4 cut(s) 151, 463, 1305, 1443
RseI CAYNNNNRTG 4 cut(s) 366, 695, 1119, 1300
SacI GAGCTC 1 cut(s) 175
SaqAI TTAA 5 cut(s) 53, 74, 414, 822, 1434
SatI GCNGC 5 cut(s) 450, 453, 1203, 1292, 1358
Sau96I GGNCC 2 cut(s) 124, 418
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 1383
SduI GDGCHC 2 cut(s) 175, 289
SexAI ACCWGGT 1 cut(s) 1381
SfaNI GCATC 2 cut(s) 358, 436
SfcI CTRYAG 4 cut(s) 679, 907, 1017, 1168
SmiMI CAYNNNNRTG 4 cut(s) 366, 695, 1119, 1300
SmlI CTYRAG 3 cut(s) 33, 413, 613
SmoI CTYRAG 3 cut(s) 33, 413, 613
SpeI ACTAGT 1 cut(s) 874
Sse9I AATT 6 cut(s) 163, 525, 649, 723, 777, 1102
SsiI CCGC 3 cut(s) 586, 828, 1329
SspMI CTAG 2 cut(s) 774, 875
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 1 cut(s) 1381
StyI CCWWGG 2 cut(s) 108, 1295
TaaI ACNGT 4 cut(s) 179, 558, 788, 911
TaiI ACGT 1 cut(s) 960
TaqI TCGA 1 cut(s) 1419
TaqII GACCGA 1 cut(s) 206
TasI AATT 6 cut(s) 163, 525, 649, 723, 777, 1102
TatI WGTACW 3 cut(s) 150, 1304, 1442
TfiI GAWTC 2 cut(s) 758, 992
Tru1I TTAA 5 cut(s) 53, 74, 414, 822, 1434
Tru9I TTAA 5 cut(s) 53, 74, 414, 822, 1434
TscAI CASTG 8 cut(s) 54, 184, 192, 499, 745, 916, 1071, 1162
TseFI GTSAC 3 cut(s) 1110, 1258, 1333
TseI GCWGC 5 cut(s) 449, 452, 1202, 1291, 1357
Tsp45I GTSAC 3 cut(s) 1110, 1258, 1333
TspDTI ATGAA 6 cut(s) 41, 56, 176, 659, 1091, 1109
TspRI CASTG 8 cut(s) 54, 184, 192, 499, 745, 916, 1071, 1162
Van91I CCANNNNNTGG 2 cut(s) 431, 1238
Vha464I CTTAAG 1 cut(s) 413
XagI CCTNNNNNAGG 1 cut(s) 1365
XapI RAATTY 1 cut(s) 649
XceI RCATGY 4 cut(s) 897, 1118, 1252, 1305
XcmI CCANNNNNNNNNTGG 2 cut(s) 309, 1235
XspI CTAG 2 cut(s) 774, 875
ZraI GACGTC 1 cut(s) 958
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.