Rorug01G0312200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009716
Physical Location & Seq
Forward (+)
42998704 .. 42999603
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0312200.1

Sequence Viewer

Length: 900 bp
ATGGATGTCTTGAGTTGGAATTGCAGGGGAATCTGCAACACAACTACTACAAGGGCACTGAGGGATTTGATCACACAGAATCAGCCTCAGATTGTCTTTCCATGTGAAACAAAAATCAGTACTTTGAGGGATTTCAAAGCGTTGCACAAGGATCTAGGGTTTGCGCACTCGAAAGAGGTCTTGAGCGTTGGTCAGGCGGGAGGCCTGGCGTTATTCTGGACAGAGGATGTCGATCTGCATGTGTGTACTGTCTCTGCGCATCACATCGACGCAGAAATTAGGGGTGCACCTGGTGAACTGTGGTGGAGAATAACAGGGTTCTATGGTCATGCAAGGACAAGAGATCGTGGGGATTCTTGGCAGCTTCTCCGTGATTTGGCTGATTTAGATTCTCTTTCTTGGGTGATAATCGGCGACTTTAATGAAATACTGAACAATAGTGAGAAGTTAGGAGGGCCTTTGCGTAGTGAAAGACAGATGCGGGGTTTCCGTGATGCATTGGGATATGGTGATCTTGCTGATCTAGGGTTTCAGGTTGCAAAGATGACCTGGTGGAATTCTGAGACTCAAATACGTTTGGATAGAGCAGTTGGTACCTCTACATGGAGTGACATCTTTGGATATTCAAGGGTGAAGCACTTACCGCCCAGTGATTCTGACCACGTTCCCATTCTGTTACAAGCTAGTTCTGTACCTCTGCCCAAGCGTCCTAAGCGGCACAGGTTTAAATTCGAGTCCTTTTGGTTGCAGCACCAAGAGTGTGATCCTCTTGTGTTATCTAATTGGAAAGCAGAAGATGGAGGTGCTCCTATGTTTGTACTAACAAGAAAGATCATTAATGACAGGACCCGCCCCGAATTTCACCCTTGTACCTACATGTACGTTGCAAAGCATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

34.19

Weight (kDa)

7.19

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 4 - 221 3.3e-12 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 165, 258
Acc65I GGTACC 1 cut(s) 593
AccB1I GGYRCC 1 cut(s) 593
AciI CCGC 5 cut(s) 197, 481, 644, 715, 850
AclWI GGATC 2 cut(s) 159, 758
AcsI RAATTY 3 cut(s) 556, 728, 857
AdeI CACNNNGTG 1 cut(s) 293
AfaI GTAC 7 cut(s) 121, 247, 595, 693, 819, 871, 881
AfiI CCNNNNNNNGG 2 cut(s) 376, 603
AflIII ACRYGT 1 cut(s) 876
AgsI TTSAA 2 cut(s) 136, 627
AjnI CCWGG 3 cut(s) 204, 289, 548
AluBI AGCT 2 cut(s) 364, 683
AluI AGCT 2 cut(s) 364, 683
Alw21I GWGCWC 2 cut(s) 289, 808
Alw26I GTCTC 2 cut(s) 256, 557
Alw44I GTGCAC 1 cut(s) 285
AlwI GGATC 2 cut(s) 159, 758
AoxI GGCC 2 cut(s) 202, 455
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 361, 748
ApoI RAATTY 3 cut(s) 556, 728, 857
AseI ATTAAT 1 cut(s) 837
Asp718I GGTACC 1 cut(s) 593
AspLEI GCGC 2 cut(s) 166, 259
AspS9I GGNCC 2 cut(s) 455, 846
AsuHPI GGTGA 5 cut(s) 305, 415, 521, 643, 854
AvaII GGWCC 1 cut(s) 846
BaeGI GKGCMC 2 cut(s) 58, 289
BaeI ACNNNNGTAYC 2 cut(s) 853, 886
BanI GGYRCC 1 cut(s) 593
Bbv12I GWGCWC 2 cut(s) 289, 808
BbvI GCAGC 2 cut(s) 373, 760
BccI CCATC 1 cut(s) 791
BciT130I CCWGG 3 cut(s) 206, 291, 550
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 256, 557
BfaI CTAG 3 cut(s) 155, 524, 684
BisI GCNGC 3 cut(s) 362, 716, 749
BlsI GCNGC 3 cut(s) 363, 717, 750
BmcAI AGTACT 1 cut(s) 121
Bme1390I CCNGG 3 cut(s) 206, 291, 550
Bme18I GGWCC 1 cut(s) 846
BmgT120I GGNCC 2 cut(s) 455, 846
BmiI GGNNCC 2 cut(s) 595, 848
BmrFI CCNGG 3 cut(s) 206, 291, 550
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 3 cut(s) 268, 468, 484
BmuI ACTGGG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 31, 202
BsaBI GATNNNNATC 1 cut(s) 231
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 603
Bse1I ACTGG 1 cut(s) 648
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 3 cut(s) 206, 291, 550
BseGI GGATG 2 cut(s) 10, 232
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 2 cut(s) 376, 603
BseMII CTCAG 3 cut(s) 50, 101, 552
BseNI ACTGG 1 cut(s) 648
BseSI GKGCMC 2 cut(s) 58, 289
BseXI GCAGC 2 cut(s) 373, 760
BshFI GGCC 2 cut(s) 204, 457
BshNI GGYRCC 1 cut(s) 593
BsiHKAI GWGCWC 2 cut(s) 289, 808
BslI CCNNNNNNNGG 2 cut(s) 376, 603
BsmAI GTCTC 2 cut(s) 256, 557
BsnI GGCC 2 cut(s) 204, 457
Bsp1286I GDGCHC 3 cut(s) 58, 289, 808
Bsp143I GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
BspACI CCGC 5 cut(s) 197, 481, 644, 715, 850
BspANI GGCC 2 cut(s) 204, 457
BspCNI CTCAG 3 cut(s) 51, 100, 553
BspLI GGNNCC 2 cut(s) 595, 848
BspPI GGATC 2 cut(s) 159, 758
BspT107I GGYRCC 1 cut(s) 593
BsrI ACTGG 1 cut(s) 648
BssMI GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
Bst2UI CCWGG 3 cut(s) 206, 291, 550
Bst4CI ACNGT 2 cut(s) 250, 300
BstDEI CTNAG 4 cut(s) 59, 87, 561, 711
BstF5I GGATG 2 cut(s) 10, 232
BstHHI GCGC 2 cut(s) 166, 259
BstKTI GATC 8 cut(s) 72, 154, 235, 346, 514, 523, 766, 834
BstMAI GTCTC 2 cut(s) 256, 557
BstMBI GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
BstMWI GCNNNNNNNGC 2 cut(s) 643, 712
BstNI CCWGG 3 cut(s) 206, 291, 550
BstNSI RCATGY 2 cut(s) 242, 880
BstSCI CCNGG 3 cut(s) 204, 289, 548
BstSLI GKGCMC 2 cut(s) 58, 289
BstV1I GCAGC 2 cut(s) 373, 760
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 2 cut(s) 204, 457
BtsCI GGATG 2 cut(s) 10, 232
BtsIMutI CAGTG 2 cut(s) 56, 655
CfoI GCGC 2 cut(s) 166, 259
Cfr13I GGNCC 2 cut(s) 455, 846
CseI GACGC 2 cut(s) 278, 695
CsiI ACCWGGT 2 cut(s) 289, 548
Csp6I GTAC 7 cut(s) 120, 246, 594, 692, 818, 870, 880
CviAII CATG 5 cut(s) 102, 239, 329, 603, 877
CviJI RGCY 6 cut(s) 85, 204, 364, 380, 457, 683
CviKI_1 RGCY 6 cut(s) 85, 204, 364, 380, 457, 683
CviQI GTAC 7 cut(s) 120, 246, 594, 692, 818, 870, 880
DdeI CTNAG 4 cut(s) 59, 87, 561, 711
DpnI GATC 8 cut(s) 71, 153, 234, 345, 513, 522, 765, 833
DpnII GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
DraI TTTAAA 1 cut(s) 727
DraIII CACNNNGTG 1 cut(s) 293
Eco147I AGGCCT 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 846
EcoO109I RGGNCCY 2 cut(s) 455, 846
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 204, 289, 548
EcoT22I ATGCAT 1 cut(s) 499
FaeI CATG 5 cut(s) 105, 242, 332, 606, 880
FaiI YATR 9 cut(s) 103, 240, 324, 330, 507, 604, 812, 878, 894
FatI CATG 5 cut(s) 101, 238, 328, 602, 876
FauI CCCGC 3 cut(s) 190, 474, 857
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 362, 716, 749
FokI GGATG 2 cut(s) 17, 239
Fsp4HI GCNGC 3 cut(s) 362, 716, 749
FspBI CTAG 3 cut(s) 155, 524, 684
FspI TGCGCA 2 cut(s) 165, 258
GlaI GCGC 2 cut(s) 165, 258
GluI GCNGC 3 cut(s) 362, 716, 749
HaeIII GGCC 2 cut(s) 204, 457
HgaI GACGC 2 cut(s) 278, 695
HhaI GCGC 2 cut(s) 166, 259
Hin1II CATG 5 cut(s) 105, 242, 332, 606, 880
Hin6I GCGC 2 cut(s) 164, 257
HinP1I GCGC 2 cut(s) 164, 257
HinfI GANTC 7 cut(s) 30, 79, 353, 389, 565, 653, 734
HphI GGTGA 5 cut(s) 305, 415, 521, 643, 854
Hpy166II GTNNAC 3 cut(s) 246, 287, 296
Hpy188I TCNGA 3 cut(s) 90, 562, 658
Hpy188III TCNNGA 3 cut(s) 10, 181, 217
Hpy8I GTNNAC 3 cut(s) 246, 287, 296
Hpy99I CGWCG 1 cut(s) 272
HpyCH4III ACNGT 2 cut(s) 250, 300
HpyCH4IV ACGT 3 cut(s) 574, 663, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 643, 712
HpyF3I CTNAG 4 cut(s) 59, 87, 561, 711
HpySE526I ACGT 3 cut(s) 574, 663, 882
Hsp92II CATG 5 cut(s) 105, 242, 332, 606, 880
HspAI GCGC 2 cut(s) 164, 257
KpnI GGTACC 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 2 cut(s) 373, 760
LweI GCATC 3 cut(s) 268, 468, 484
MabI ACCWGGT 2 cut(s) 289, 548
MaeI CTAG 3 cut(s) 155, 524, 684
MaeII ACGT 3 cut(s) 574, 663, 882
MaeIII GTNAC 2 cut(s) 608, 675
MalI GATC 8 cut(s) 71, 153, 234, 345, 513, 522, 765, 833
MboI GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
MboII GAAGA 1 cut(s) 806
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 3 cut(s) 58, 289, 808
MluCI AATT 7 cut(s) 19, 276, 556, 728, 781, 857, 895
MlyI GAGTC 2 cut(s) 559, 743
Mph1103I ATGCAT 1 cut(s) 499
MseI TTAA 3 cut(s) 420, 726, 837
MspR9I CCNGG 3 cut(s) 206, 291, 550
MvaI CCWGG 3 cut(s) 206, 291, 550
MwoI GCNNNNNNNGC 2 cut(s) 643, 712
NdeII GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
NlaIII CATG 5 cut(s) 105, 242, 332, 606, 880
NlaIV GGNNCC 2 cut(s) 595, 848
NmuCI GTSAC 1 cut(s) 608
NsbI TGCGCA 2 cut(s) 165, 258
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 2 cut(s) 242, 880
PceI AGGCCT 1 cut(s) 204
PciI ACATGT 1 cut(s) 876
PfeI GAWTC 5 cut(s) 30, 79, 353, 389, 653
PkrI GCNGC 3 cut(s) 363, 717, 750
PleI GAGTC 2 cut(s) 559, 742
PpsI GAGTC 2 cut(s) 559, 742
PpuMI RGGWCCY 1 cut(s) 846
PscI ACATGT 1 cut(s) 876
PshBI ATTAAT 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 846
Psp6I CCWGG 3 cut(s) 204, 289, 548
PspGI CCWGG 3 cut(s) 204, 289, 548
PspN4I GGNNCC 2 cut(s) 595, 848
PspPI GGNCC 2 cut(s) 455, 846
PspPPI RGGWCCY 1 cut(s) 846
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 7 cut(s) 121, 247, 595, 693, 819, 871, 881
RsaNI GTAC 7 cut(s) 120, 246, 594, 692, 818, 870, 880
SaqAI TTAA 3 cut(s) 420, 726, 837
SatI GCNGC 3 cut(s) 362, 716, 749
Sau3AI GATC 8 cut(s) 69, 151, 232, 343, 511, 520, 763, 831
Sau96I GGNCC 2 cut(s) 455, 846
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 2 cut(s) 559, 743
ScrFI CCNGG 3 cut(s) 206, 291, 550
SduI GDGCHC 3 cut(s) 58, 289, 808
SexAI ACCWGGT 2 cut(s) 289, 548
SfaNI GCATC 3 cut(s) 268, 468, 484
SinI GGWCC 1 cut(s) 846
SmlI CTYRAG 2 cut(s) 10, 181
SmoI CTYRAG 2 cut(s) 10, 181
Sse9I AATT 7 cut(s) 19, 276, 556, 728, 781, 857, 895
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 5 cut(s) 197, 481, 644, 715, 850
SspMI CTAG 3 cut(s) 155, 524, 684
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 3 cut(s) 204, 289, 548
TaaI ACNGT 2 cut(s) 250, 300
TaiI ACGT 3 cut(s) 577, 666, 885
TaqI TCGA 4 cut(s) 170, 231, 267, 732
TasI AATT 7 cut(s) 19, 276, 556, 728, 781, 857, 895
TatI WGTACW 3 cut(s) 119, 245, 817
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 5 cut(s) 30, 79, 353, 389, 653
Tru1I TTAA 3 cut(s) 420, 726, 837
Tru9I TTAA 3 cut(s) 420, 726, 837
TscAI CASTG 2 cut(s) 63, 655
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 2 cut(s) 361, 748
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 1 cut(s) 438
TspGWI ACGGA 2 cut(s) 359, 479
TspRI CASTG 2 cut(s) 63, 655
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 846
VspI ATTAAT 1 cut(s) 837
XapI RAATTY 3 cut(s) 556, 728, 857
XceI RCATGY 2 cut(s) 242, 880
XspI CTAG 3 cut(s) 155, 524, 684
ZrmI AGTACT 1 cut(s) 121
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.