Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0011597

Physical Location & Seq

Genomic Coordinates Reverse (-)

50395041 .. 50403837

Gene Structure 8797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0393600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3792 bp

ATGAATCGTACGGTCGGTGAGTCCTCCTCCGCCCTCGCATCACCGTCCACCTCACACTCAATTCGCGCCTCACCATCGTCGTCGTCCGAAACGTTTCAGAAACCGAAACCAAACGCCATATTCTTGTTTTTCCTTCCCTCCTTTCAAATCAGACCAAGAACACGAAACAGTAGCTGCATTATCATCACTATGTTGGCTAAACTCTTCAAAAGACCCTCACAAAATCAACATCATCCTCAGGCAAGGGTGTCACAAGCTGATTTGGGTCCAAGAATTGTTTTTCACTATGGGATTCCATCTACTGCATCGATTCTCGCACTTGATCGCACTCAGAGCTTGCTGGCAATTGGAACCCTGGATGGGAGGATAAAAGTGATTGGTGGCGATAATATTGAAGGGCTTCTGATATCTCCTGAGCCATTGGCATTTAAATACTTGGAGTTCTTAGAAAATCAAGGTTTTTTAGCTAGTGTCTCAAGTGAAAATGAAATTCAGGTTTGGGATTTGGAACAGAAGCAAATAGCCGGTTCTTTACAGTGGGAATGCAATATAACTGCCTTCTCTGTTATTTATGGCACCAATTACATGTATATTGGAAGCGAATATGCAATGGTGTCTATGGTGAAGTATGATGCTGAAGATCGAAAAATCAATTTACTGCCATATTATATTACTGCAAACTTCATAGCTGAAGCAGCTGGAATGTCATTGCCTGATAATCTTTCTGTTGTTGGAGTTCTCCATCAACCTAGTTCTCAGGGAAACAGACTGCTGATTGCATATGAGAATGGTCTGATTGTTCTCTGGGATGCTTTAGAAGATCGAGTTGTGCTTGTAAGAGGTTCCAAGGACCTGCAAGTGAAGGAAGAAACAGTCCATAATTCTTTTGAAGGCACAAGAACTGAGCTGACTGATGCTACATCAGACAGCAAACAGGTGGAGAAAGAGATAAGCTCTCTTTGTTGGGTATGCGATAATGGGTCAAGTCTTGCTGTTGGCTATGTAGATGGTGATATAATGTTTTGGGATATATCAACTGCTGAGTCTACCAAGGATCATAGATCTAAAAAGTTAGCTAACAATGTTGCGAAGCTAGAGTTATCATCAGATGACAAGAGACTCCCTGTCATAGTTTTACACTGGTCTGCAAATAGGTTAAGTCATCATCCTAGGGGCCAACTCTTTGTTTATGGAGGTGAAGGAATTGGGTCTGAAGAAGTTCTCACGGTTCTGAGCGTGGATTGGTCTTCAGGAATAGAAAGTCTGAAATGCATAGGTCGTGTGGACCTTACACTTGTTGGTTCATTTGCAGATATGGTTTTACTTCCAGCTGCTGGTGCCATGGAGAACGGTGACACATTGCTATTCACTTTGACAAATCAGGGACAACTGCATGTCTATGACAAGGCTTGCTGGTCTGCCTTAATGTCTCACCAAAAGAAAAGGACTGATGGGACTGCAGTGCAGTATCCTATGTTTATACCTACTACTGAACCGTGTATGACAGTGGCAAAGCTTGCTTTGGTGGATAGAGATGGGAAATACTCCTCAGCTCTGTCCAAGGAAGTTTCTGTTGACAAGTCTAATGCGAAACACACTTTGAAGAAGGGGGGTGCAAAGTGGCCTTTGACTGGTGGTGTTCCCAGCCAACTTTCTGATGCTGAAAATTTTCATGTCGAGAGACTATATGTAGCAGGATATCAAGATGGATCTGTTCGAATATGGGATGCCACCTATCCAACTCCGTCGCTTATATATGCTATAGGTCCTGAGGTAAAAGGCATCAGATCTACAGGTGCAAATGGAACAGTCTCAGCATTGGAATTTTGTTCTCTTACTTTAAAATTAGCTATTGGTGATGTGTGTGGTCTGGTTCGTCTATATAAACTAATAGGGAGTTCAGACGAGGCAGAATTGCACTTTGTGACAAAAACAGAGAAGGAAGTTTATACTTCACAACAAGGTAATGGGCCTCAGTGCACAGTTGTGTTTTCTATTCTTGATTCTCCTATATCCACACTACAATATGCAAATTTTGGAGCAAGACTGAGTGTGGGATATGAATGTGGCCGGGTTGCAATGCTTGATATTAGTACATCGTCAGTTTTGTTTCTTACAGACAGTGTCTCCAACTCAATTTCCCCAGTCATCTGTCTGTCTGTGAAATCATTTTCAGATACTAATAGCTTATCACAGAGCCCAAAGGGCTCTGAATCCAAGAATTCGACTGATCCTCAAAATGGAATGAATCCAAAGGATTCTGAATCCAAAACTTTAAGTGATTCTGGAAATGGGTTGGCAATCCCAAAGGACTCTGAATCCAAGAATGTGGCTGACCCTGAACATGGAATGAGTGTAAAAGATACTGAATTTAAGACTTTGAGTGATTCTGGAAATGGGTTGGCAATCCCAGAGAACTCTGAATCCAAGAATGTGGCTGATCCTGAACCTGGAATGAGTGTAAAAGATCCTGCATCCCAGACTTTGAGTGATTCTGAAAATGGGTTGGCGTTCATTATGACCAGAAATGCACATTTTGTTATTTTAGATAGTGCCACTGGCAATATGATCAGGTCTTGGCCCATGCATCCAAAAATGGATTCTACTGCAATTTCGATGTACATTATAGAGGATGGTGATGTTTTATTTGATTTGTCGAGTGCAAAAAAGGATTCTTTAGACTTACCTCAGAAAGGCGTAGCTAAACATGATGACGTACCACCTAATGCTGACTCTGGAGGGACACAGCTTGTAGTTGACCAGGATACCTCCACCAAAACTGCATATTTTTTGCAGAGATCGGTGAACATATGTGTTTTACTTTGTTGTGACTACACATTGCACTTATGTTCATTGAAGTCTCTGCTTGAGGGTGACAGTAACTGCATTCTGAAGGTGAATCTTGTAAAACCGTGTTGTTGGACTACAATCTTCAAAAAAGATGGAAAAGACAGTGGATTGGTTATACTAAATCAGACAGGAGTCTTTGAGATCAGGTCTTTTCCAAATCTTGAAGTGGTGAGAGATATATCATTAATGACGATTCTAAGATGGAACTTCGTGATCAACATGGATAAGACATTCTGTTCCTCTGATCGTGGGCAAATGATTCTGGTACATGGGTGTGAATTAGCTTTTGTCTCTATTTTGGCCTACGAAGATGACTTCAGGATTTCGGAGTCCTTCCCTTGTCTTCATGATAAAGTGCTTGCAGCTGCTACAGATGTCATTGCCGATTTATCTCAAAGACAGAAACAGAGTGCTGCCCATAGGATTTTAGGAGGTATCATTAAGGGCTTAAAAACTAGTAAAACGGATCAAAACATGGACCCGACTGGAAATCATGAAAAATATTGTGCGAATTTGGAGAGCTTATTTTCAAATCCCCCATTCTTAAAACCATCCACAGATGTGAAGGATGACCAAGAACCTGTGGTGCTTAACCTAGATGATATTGTAATTGATGGCCCTATTACTGTCTTGCCTAAAGATCCAAACAAGAAGAATGAAAAGAAAGACAAGGGATCAGAGAAGAAAAAGTTATTTGAAGGCGCAACTAGTGGTACAAAGCCAAAAATGAGGACAGCTGCCGAAATCAAGGCTAAATATAGAGAAACTGGGGATGTCTTTGCAGCAGCTGCACGTGCAAGAGATATGCTTGCCGAACGTGGGGAAAAACTTGAGAAACTCAGAGAAAACACAGAAGAACTATCGAGTGGAGCTCAAGACTTCGCATCGATGGCAAAGGAACTCGCCAAGAGAATGGAGAATCGTAAATGGTGGCACATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

1263

Amino Acids

138.24

Weight (kDa)

5.59

Isoelectric Point (pI)

37.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Synaptobrevin	PF00957	1219 - 1260	2e-06	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0011597)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g28590
malus_domestica	MD12G1112400.v1.1
prunus_persica	Prupe.2G285900_v2.0.a1 Prupe.2G285900_v2.0.a1 Prupe.2G285900_v2.0.a1 Prupe.2G285900_v2.0.a1
pyrus_communis	pycom01g20270 pycom07g23840
rosa_chinensis	RchiOBHm_Chr1g0376001
rosa_laevigata	RLG00000026636
rosa_multiflora	Rmu_ssc0000116.1_g000013
rosa_roxburghii	Rroxscaffold_4G00282430
rosa_rugosa	Rorug01G0393600 Rorug01G0393600 Rorug01G0393600
rosa_samantha	Rh1AG404900 Rh1BG368400 Rh1CG381300 Rh1DG398300
rosa_wichuraiana	Rw1G035910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	861
AccB1I	GGYRCC	2 cut(s)	575, 1337
AccB7I	CCANNNNNTGG	1 cut(s)	1334
AccI	GTMKAC	1 cut(s)	1046
AccII	CGCG	1 cut(s)	66
AciI	CCGC	1 cut(s)	30
AclI	AACGTT	1 cut(s)	92
AclWI	GGATC	8 cut(s)	1062, 1717, 2225, 2435, 2462, 3324, 3485, 3532
AcoI	YGGCCR	1 cut(s)	2068
AcsI	RAATTY	7 cut(s)	489, 1666, 1823, 2032, 2221, 2369, 3361
AcuI	CTGAAG	6 cut(s)	657, 711, 1233, 1233, 2912, 3151
AcvI	CACGTG	1 cut(s)	3644
AdeI	CACNNNGTG	1 cut(s)	1924
AfaI	GTAC	6 cut(s)	10, 2095, 2621, 2718, 3117, 3565
AfiI	CCNNNNNNNGG	8 cut(s)	360, 1334, 1631, 2240, 2345, 2450, 2693, 3669
AflIII	ACRYGT	1 cut(s)	585
AhdI	GACNNNNNGTC	1 cut(s)	1124
AhlI	ACTAGT	2 cut(s)	3305, 3557
AjnI	CCWGG	3 cut(s)	354, 2449, 2760
AjuI	GAANNNNNNNTTGG	2 cut(s)	2584, 2616
AleI	CACNNNNGTG	2 cut(s)	1985, 3410
AloI	GAACNNNNNNTCC	2 cut(s)	2408, 2440
Alw21I	GWGCWC	2 cut(s)	1982, 3724
Alw26I	GTCTC	8 cut(s)	478, 1111, 1434, 1675, 1816, 2131, 2865, 3145
Alw44I	GTGCAC	1 cut(s)	1978
AlwI	GGATC	8 cut(s)	1062, 1717, 2225, 2435, 2462, 3324, 3485, 3532
AlwNI	CAGNNNCTG	4 cut(s)	174, 1334, 2883, 3638
AoxI	GGCC	7 cut(s)	1174, 1622, 1970, 2068, 2579, 3150, 3466
ApaLI	GTGCAC	1 cut(s)	1978
ApoI	RAATTY	7 cut(s)	489, 1666, 1823, 2032, 2221, 2369, 3361
AseI	ATTAAT	1 cut(s)	3035
Asp700I	GAANNNNTTC	4 cut(s)	93, 399, 1218, 1668
AspA2I	CCTAGG	1 cut(s)	1169
AspLEI	GCGC	2 cut(s)	68, 3554
AspS9I	GGNCC	9 cut(s)	266, 850, 1174, 1285, 1766, 1970, 2580, 3328, 3467
AsuC2I	CCSGG	1 cut(s)	2072
AsuII	TTCGAA	1 cut(s)	1717
AvaII	GGWCC	5 cut(s)	266, 850, 1285, 1766, 3328
AvrII	CCTAGG	1 cut(s)	1169
AxyI	CCTNAGG	2 cut(s)	237, 1770
BaeGI	GKGCMC	1 cut(s)	1982
BaeI	ACNNNNGTAYC	2 cut(s)	3107, 3140
BanI	GGYRCC	2 cut(s)	575, 1337
BanII	GRGCYC	3 cut(s)	2201, 2210, 3724
BbrPI	CACGTG	1 cut(s)	3644
BbsI	GAAGAC	2 cut(s)	1239, 3185
Bbv12I	GWGCWC	2 cut(s)	1982, 3724
BbvCI	CCTCAGC	1 cut(s)	1549
BciT130I	CCWGG	3 cut(s)	356, 2451, 2762
BciVI	GTATCC	2 cut(s)	1479, 2758
BclI	TGATCA	2 cut(s)	2568, 3063
BcnI	CCSGG	1 cut(s)	2072
BcoDI	GTCTC	8 cut(s)	478, 1111, 1434, 1675, 1816, 2131, 2865, 3145
BcuI	ACTAGT	2 cut(s)	3305, 3557
BfaI	CTAG	7 cut(s)	468, 750, 1094, 1170, 3306, 3446, 3558
BfmI	CTRYAG	4 cut(s)	1458, 1761, 1791, 3219
BfuAI	ACCTGC	1 cut(s)	861
BfuI	GTATCC	2 cut(s)	1479, 2758
BglI	GCCNNNNNGGC	1 cut(s)	2205
BglII	AGATCT	2 cut(s)	1061, 1787
BlnI	CCTAGG	1 cut(s)	1169
Bme1390I	CCNGG	4 cut(s)	356, 2072, 2451, 2762
Bme18I	GGWCC	5 cut(s)	266, 850, 1285, 1766, 3328
BmeRI	GACNNNNNGTC	1 cut(s)	1124
BmgT120I	GGNCC	9 cut(s)	266, 850, 1174, 1285, 1766, 1970, 2580, 3328, 3467
BmiI	GGNNCC	7 cut(s)	267, 352, 577, 844, 1175, 1339, 3330
BmrFI	CCNGG	4 cut(s)	356, 2072, 2451, 2762
BmrI	ACTGGG	2 cut(s)	2138, 3627
BmuI	ACTGGG	2 cut(s)	2138, 3627
BoxI	GACNNNNGTC	1 cut(s)	2981
BpiI	GAAGAC	2 cut(s)	1239, 3185
BplI	GAGNNNNNCTC	6 cut(s)	11, 43, 938, 970, 3706, 3738
BpmI	CTGGAG	1 cut(s)	2757
Bpu10I	CCTNAGC	2 cut(s)	414, 1549
Bpu14I	TTCGAA	1 cut(s)	1717
BpuEI	CTTGAG	4 cut(s)	460, 2888, 3701, 3708
BpuMI	CCSGG	1 cut(s)	2072
Bsa29I	ATCGAT	2 cut(s)	308, 3737
BsaAI	YACGTR	1 cut(s)	3644
BsaJI	CCNNGG	6 cut(s)	354, 846, 1050, 1169, 1341, 1560
BsaXI	ACNNNNNCTCC	2 cut(s)	2111, 2141
Bsc4I	CCNNNNNNNGG	8 cut(s)	360, 1334, 1631, 2240, 2345, 2450, 2693, 3669
Bse118I	RCCGGY	1 cut(s)	524
Bse1I	ACTGG	6 cut(s)	1145, 1636, 2144, 2563, 3340, 3622
Bse21I	CCTNAGG	2 cut(s)	237, 1770
Bse3DI	GCAATG	6 cut(s)	615, 707, 1358, 2085, 2837, 3228
BseBI	CCWGG	3 cut(s)	356, 2451, 2762
BseCI	ATCGAT	2 cut(s)	308, 3737
BseDI	CCNNGG	6 cut(s)	354, 846, 1050, 1169, 1341, 1560
BseLI	CCNNNNNNNGG	8 cut(s)	360, 1334, 1631, 2240, 2345, 2450, 2693, 3669
BseMI	GCAATG	6 cut(s)	615, 707, 1358, 2085, 2837, 3228
BseNI	ACTGG	6 cut(s)	1145, 1636, 2144, 2563, 3340, 3622
BseRI	GAGGAG	2 cut(s)	16, 1537
BseSI	GKGCMC	1 cut(s)	1982
BseYI	CCCAGC	1 cut(s)	1643
BsgI	GTGCAG	2 cut(s)	1484, 3624
Bsh1236I	CGCG	1 cut(s)	66
Bsh1285I	CGRYCG	1 cut(s)	15
BshFI	GGCC	7 cut(s)	1176, 1624, 1972, 2070, 2581, 3152, 3468
BshNI	GGYRCC	2 cut(s)	575, 1337
BshVI	ATCGAT	2 cut(s)	308, 3737
BsiEI	CGRYCG	1 cut(s)	15
BsiHKAI	GWGCWC	2 cut(s)	1982, 3724
BsiSI	CCGG	2 cut(s)	525, 2071
BsiWI	CGTACG	1 cut(s)	8
BslFI	GGGAC	3 cut(s)	1398, 1468, 2755
BslI	CCNNNNNNNGG	8 cut(s)	360, 1334, 1631, 2240, 2345, 2450, 2693, 3669
BsmAI	GTCTC	8 cut(s)	478, 1111, 1434, 1675, 1816, 2131, 2865, 3145
BsmFI	GGGAC	3 cut(s)	1398, 1468, 2755
BsmI	GAATGC	2 cut(s)	548, 2886
BsnI	GGCC	7 cut(s)	1176, 1624, 1972, 2070, 2581, 3152, 3468
Bsp119I	TTCGAA	1 cut(s)	1717
Bsp1286I	GDGCHC	4 cut(s)	1982, 2201, 2210, 3724
Bsp1407I	TGTACA	1 cut(s)	2619
Bsp19I	CCATGG	1 cut(s)	1341
BspACI	CCGC	1 cut(s)	30
BspANI	GGCC	7 cut(s)	1176, 1624, 1972, 2070, 2581, 3152, 3468
BspDI	ATCGAT	2 cut(s)	308, 3737
BspFNI	CGCG	1 cut(s)	66
BspHI	TCATGA	2 cut(s)	3196, 3343
BspLI	GGNNCC	7 cut(s)	267, 352, 577, 844, 1175, 1339, 3330
BspMAI	CTGCAG	1 cut(s)	1462
BspMI	ACCTGC	1 cut(s)	861
BspPI	GGATC	8 cut(s)	1062, 1717, 2225, 2435, 2462, 3324, 3485, 3532
BspT104I	TTCGAA	1 cut(s)	1717
BspT107I	GGYRCC	2 cut(s)	575, 1337
BsrDI	GCAATG	6 cut(s)	615, 707, 1358, 2085, 2837, 3228
BsrFI	RCCGGY	1 cut(s)	524
BsrGI	TGTACA	1 cut(s)	2619
BsrI	ACTGG	6 cut(s)	1145, 1636, 2144, 2563, 3340, 3622
BssAI	RCCGGY	1 cut(s)	524
BssECI	CCNNGG	6 cut(s)	354, 846, 1050, 1169, 1341, 1560
BssT1I	CCWWGG	5 cut(s)	846, 1050, 1169, 1341, 1560
Bst2UI	CCWGG	3 cut(s)	356, 2451, 2762
Bst6I	CTCTTC	1 cut(s)	209
BstAPI	GCANNNNNTGC	2 cut(s)	1517, 3638
BstAUI	TGTACA	1 cut(s)	2619
BstBAI	YACGTR	1 cut(s)	3644
BstBI	TTCGAA	1 cut(s)	1717
BstC8I	GCNNGC	6 cut(s)	338, 342, 1411, 1518, 3210, 3660
BstDSI	CCRYGG	1 cut(s)	1341
BstENI	CCTNNNNNAGG	1 cut(s)	2691
BstFNI	CGCG	1 cut(s)	66
BstHHI	GCGC	2 cut(s)	68, 3554
BstMAI	GTCTC	8 cut(s)	478, 1111, 1434, 1675, 1816, 2131, 2865, 3145
BstMCI	CGRYCG	1 cut(s)	15
BstMWI	GCNNNNNNNGC	8 cut(s)	333, 695, 1337, 1517, 2205, 3638, 3644, 3740
BstNI	CCWGG	3 cut(s)	356, 2451, 2762
BstNSI	RCATGY	2 cut(s)	589, 1397
BstPAI	GACNNNNGTC	1 cut(s)	2981
BstSCI	CCNGG	4 cut(s)	354, 2070, 2449, 2760
BstSFI	CTRYAG	4 cut(s)	1458, 1761, 1791, 3219
BstSLI	GKGCMC	1 cut(s)	1982
BstUI	CGCG	1 cut(s)	66
BstV2I	GAAGAC	2 cut(s)	1239, 3185
BstX2I	RGATCY	5 cut(s)	1061, 1709, 1787, 2467, 3490
BstXI	CCANNNNNNTGG	3 cut(s)	2329, 2434, 3763
BstYI	RGATCY	5 cut(s)	1061, 1709, 1787, 2467, 3490
Bsu15I	ATCGAT	2 cut(s)	308, 3737
Bsu36I	CCTNAGG	2 cut(s)	237, 1770
BsuI	GTATCC	2 cut(s)	1479, 2758
BsuRI	GGCC	7 cut(s)	1176, 1624, 1972, 2070, 2581, 3152, 3468
BsuTUI	ATCGAT	2 cut(s)	308, 3737
BtgI	CCRYGG	1 cut(s)	1341
BtsI	GCAGTG	1 cut(s)	1467
BtsIMutI	CAGTG	8 cut(s)	542, 1138, 1467, 1512, 1982, 2128, 2556, 2959
BveI	ACCTGC	1 cut(s)	861
Cac8I	GCNNGC	6 cut(s)	338, 342, 1411, 1518, 3210, 3660
CaiI	CAGNNNCTG	4 cut(s)	174, 1334, 2883, 3638
CciI	TCATGA	2 cut(s)	3196, 3343
CfoI	GCGC	2 cut(s)	68, 3554
Cfr10I	RCCGGY	1 cut(s)	524
Cfr13I	GGNCC	9 cut(s)	266, 850, 1174, 1285, 1766, 1970, 2580, 3328, 3467
ClaI	ATCGAT	2 cut(s)	308, 3737
Csp6I	GTAC	6 cut(s)	9, 2094, 2620, 2717, 3116, 3564
CviQI	GTAC	6 cut(s)	9, 2094, 2620, 2717, 3116, 3564
DraI	TTTAAA	2 cut(s)	430, 1842
DraIII	CACNNNGTG	1 cut(s)	1924
DriI	GACNNNNNGTC	1 cut(s)	1124
EaeI	YGGCCR	1 cut(s)	2068
Eam1104I	CTCTTC	1 cut(s)	209
Eam1105I	GACNNNNNGTC	1 cut(s)	1124
EarI	CTCTTC	1 cut(s)	209
EciI	GGCGGA	1 cut(s)	19
Ecl136II	GAGCTC	1 cut(s)	3722
Eco130I	CCWWGG	5 cut(s)	846, 1050, 1169, 1341, 1560
Eco24I	GRGCYC	3 cut(s)	2201, 2210, 3724
Eco32I	GATATC	2 cut(s)	408, 1700
Eco47I	GGWCC	5 cut(s)	266, 850, 1285, 1766, 3328
Eco53kI	GAGCTC	1 cut(s)	3722
Eco57I	CTGAAG	6 cut(s)	657, 711, 1233, 1233, 2912, 3151
Eco72I	CACGTG	1 cut(s)	3644
Eco81I	CCTNAGG	2 cut(s)	237, 1770
EcoICRI	GAGCTC	1 cut(s)	3722
EcoNI	CCTNNNNNAGG	1 cut(s)	2691
EcoO109I	RGGNCCY	2 cut(s)	850, 1766
EcoRI	GAATTC	1 cut(s)	2221
EcoRII	CCWGG	3 cut(s)	354, 2449, 2760
EcoRV	GATATC	2 cut(s)	408, 1700
EcoT14I	CCWWGG	5 cut(s)	846, 1050, 1169, 1341, 1560
EcoT22I	ATGCAT	2 cut(s)	1274, 2589
EcoT38I	GRGCYC	3 cut(s)	2201, 2210, 3724
ErhI	CCWWGG	5 cut(s)	846, 1050, 1169, 1341, 1560
FaqI	GGGAC	3 cut(s)	1398, 1468, 2755
FauNDI	CATATG	3 cut(s)	781, 2810, 3788
FbaI	TGATCA	2 cut(s)	2568, 3063
FblI	GTMKAC	1 cut(s)	1046
FriOI	GRGCYC	3 cut(s)	2201, 2210, 3724
FspBI	CTAG	7 cut(s)	468, 750, 1094, 1170, 3306, 3446, 3558
GlaI	GCGC	2 cut(s)	67, 3553
GsaI	CCCAGC	1 cut(s)	1647
GsuI	CTGGAG	1 cut(s)	2757
HaeIII	GGCC	7 cut(s)	1176, 1624, 1972, 2070, 2581, 3152, 3468
HapII	CCGG	2 cut(s)	525, 2071
HhaI	GCGC	2 cut(s)	68, 3554
Hin6I	GCGC	2 cut(s)	66, 3552
HinP1I	GCGC	2 cut(s)	66, 3552
HincII	GTYRAC	2 cut(s)	1576, 2758
HindII	GTYRAC	2 cut(s)	1576, 2758
HindIII	AAGCTT	1 cut(s)	1514
HpaII	CCGG	2 cut(s)	525, 2071
Hpy166II	GTNNAC	7 cut(s)	48, 1047, 1285, 1576, 1980, 2758, 2806
Hpy8I	GTNNAC	7 cut(s)	48, 1047, 1285, 1576, 1980, 2758, 2806
Hpy99I	CGWCG	3 cut(s)	82, 85, 1750
HpyCH4IV	ACGT	4 cut(s)	92, 2715, 3643, 3667
HpyF10VI	GCNNNNNNNGC	8 cut(s)	333, 695, 1337, 1517, 2205, 3638, 3644, 3740
HpySE526I	ACGT	4 cut(s)	92, 2715, 3643, 3667
HspAI	GCGC	2 cut(s)	66, 3552
Ksp22I	TGATCA	2 cut(s)	2568, 3063
LmnI	GCTCC	2 cut(s)	2039, 3719
MaeI	CTAG	7 cut(s)	468, 750, 1094, 1170, 3306, 3446, 3558
MaeII	ACGT	4 cut(s)	92, 2715, 3643, 3667
MaeIII	GTNAC	6 cut(s)	249, 1352, 1924, 2828, 2873, 2879
MfeI	CAATTG	1 cut(s)	345
MflI	RGATCY	5 cut(s)	1061, 1709, 1787, 2467, 3490
MhlI	GDGCHC	4 cut(s)	1982, 2201, 2210, 3724
MlyI	GAGTC	7 cut(s)	29, 1052, 1113, 2306, 2726, 2991, 3188
MmeI	TCCRAC	4 cut(s)	712, 1763, 2154, 2900
Mph1103I	ATGCAT	2 cut(s)	1274, 2589
MroXI	GAANNNNTTC	4 cut(s)	93, 399, 1218, 1668
MslI	CAYNNNNRTG	5 cut(s)	188, 1398, 1985, 3123, 3410
MspA1I	CMGCKG	5 cut(s)	698, 1331, 3215, 3587, 3638
MspI	CCGG	2 cut(s)	525, 2071
MspR9I	CCNGG	4 cut(s)	356, 2072, 2451, 2762
MunI	CAATTG	1 cut(s)	345
Mva1269I	GAATGC	2 cut(s)	548, 2886
MvaI	CCWGG	3 cut(s)	356, 2451, 2762
MvnI	CGCG	1 cut(s)	66
MwoI	GCNNNNNNNGC	8 cut(s)	333, 695, 1337, 1517, 2205, 3638, 3644, 3740
NciI	CCSGG	1 cut(s)	2072
NcoI	CCATGG	1 cut(s)	1341
NdeI	CATATG	3 cut(s)	781, 2810, 3788
NlaIV	GGNNCC	7 cut(s)	267, 352, 577, 844, 1175, 1339, 3330
NmuCI	GTSAC	5 cut(s)	249, 1352, 1924, 2828, 2873
NsiI	ATGCAT	2 cut(s)	1274, 2589
NspI	RCATGY	2 cut(s)	589, 1397
NspV	TTCGAA	1 cut(s)	1717
OliI	CACNNNNGTG	2 cut(s)	1985, 3410
PagI	TCATGA	2 cut(s)	3196, 3343
PciI	ACATGT	1 cut(s)	585
PcsI	WCGNNNNNNNCGW	1 cut(s)	89
PctI	GAATGC	2 cut(s)	548, 2886
PdmI	GAANNNNTTC	4 cut(s)	93, 399, 1218, 1668
Pfl23II	CGTACG	1 cut(s)	8
PflFI	GACNNNGTC	1 cut(s)	2123
PflMI	CCANNNNNTGG	1 cut(s)	1334
PleI	GAGTC	7 cut(s)	28, 1051, 1113, 2306, 2726, 2990, 3187
PmaCI	CACGTG	1 cut(s)	3644
PmlI	CACGTG	1 cut(s)	3644
PpsI	GAGTC	7 cut(s)	28, 1051, 1113, 2306, 2726, 2990, 3187
Ppu21I	YACGTR	1 cut(s)	3644
PpuMI	RGGWCCY	2 cut(s)	850, 1766
PscI	ACATGT	1 cut(s)	585
PshAI	GACNNNNGTC	1 cut(s)	2981
PshBI	ATTAAT	1 cut(s)	3035
Psp124BI	GAGCTC	1 cut(s)	3724
Psp1406I	AACGTT	1 cut(s)	92
Psp5II	RGGWCCY	2 cut(s)	850, 1766
Psp6I	CCWGG	3 cut(s)	354, 2449, 2760
PspCI	CACGTG	1 cut(s)	3644
PspFI	CCCAGC	1 cut(s)	1643
PspGI	CCWGG	3 cut(s)	354, 2449, 2760
PspLI	CGTACG	1 cut(s)	8
PspN4I	GGNNCC	7 cut(s)	267, 352, 577, 844, 1175, 1339, 3330
PspPI	GGNCC	9 cut(s)	266, 850, 1174, 1285, 1766, 1970, 2580, 3328, 3467
PspPPI	RGGWCCY	2 cut(s)	850, 1766
PstI	CTGCAG	1 cut(s)	1462
PstNI	CAGNNNCTG	4 cut(s)	174, 1334, 2883, 3638
PsuI	RGATCY	5 cut(s)	1061, 1709, 1787, 2467, 3490
PsyI	GACNNNGTC	1 cut(s)	2123
PvuII	CAGCTG	5 cut(s)	698, 1331, 3215, 3587, 3638
RsaI	GTAC	6 cut(s)	10, 2095, 2621, 2718, 3117, 3565
RsaNI	GTAC	6 cut(s)	9, 2094, 2620, 2717, 3116, 3564
RseI	CAYNNNNRTG	5 cut(s)	188, 1398, 1985, 3123, 3410
SacI	GAGCTC	1 cut(s)	3724
Sau96I	GGNCC	9 cut(s)	266, 850, 1174, 1285, 1766, 1970, 2580, 3328, 3467
SchI	GAGTC	7 cut(s)	29, 1052, 1113, 2306, 2726, 2991, 3188
ScrFI	CCNGG	4 cut(s)	356, 2072, 2451, 2762
SduI	GDGCHC	4 cut(s)	1982, 2201, 2210, 3724
SfcI	CTRYAG	4 cut(s)	1458, 1761, 1791, 3219
SfuI	TTCGAA	1 cut(s)	1717
SinI	GGWCC	5 cut(s)	266, 850, 1285, 1766, 3328
SmiI	ATTTAAAT	1 cut(s)	430
SmiMI	CAYNNNNRTG	5 cut(s)	188, 1398, 1985, 3123, 3410
SmlI	CTYRAG	4 cut(s)	475, 2867, 3680, 3723
SmoI	CTYRAG	4 cut(s)	475, 2867, 3680, 3723
SpeI	ACTAGT	2 cut(s)	3305, 3557
SsiI	CCGC	1 cut(s)	30
SspI	AATATT	2 cut(s)	391, 3353
SspMI	CTAG	7 cut(s)	468, 750, 1094, 1170, 3306, 3446, 3558
SstI	GAGCTC	1 cut(s)	3724
StyD4I	CCNGG	4 cut(s)	354, 2070, 2449, 2760
StyI	CCWWGG	5 cut(s)	846, 1050, 1169, 1341, 1560
SwaI	ATTTAAAT	1 cut(s)	430
TaiI	ACGT	4 cut(s)	95, 2718, 3646, 3670
TatI	WGTACW	2 cut(s)	2093, 2619
TscAI	CASTG	8 cut(s)	542, 1145, 1467, 1512, 1982, 2128, 2563, 2959
TseFI	GTSAC	5 cut(s)	249, 1352, 1924, 2828, 2873
Tsp45I	GTSAC	5 cut(s)	249, 1352, 1924, 2828, 2873
TspGWI	ACGGA	2 cut(s)	1734, 3329
TspRI	CASTG	8 cut(s)	542, 1145, 1467, 1512, 1982, 2128, 2563, 2959
Tth111I	GACNNNGTC	1 cut(s)	2123
Van91I	CCANNNNNTGG	1 cut(s)	1334
VneI	GTGCAC	1 cut(s)	1978
VpaK11BI	GGWCC	5 cut(s)	266, 850, 1285, 1766, 3328
VspI	ATTAAT	1 cut(s)	3035
XagI	CCTNNNNNAGG	1 cut(s)	2691
XapI	RAATTY	7 cut(s)	489, 1666, 1823, 2032, 2221, 2369, 3361
XceI	RCATGY	2 cut(s)	589, 1397
XmaJI	CCTAGG	1 cut(s)	1169
XmiI	GTMKAC	1 cut(s)	1046
XmnI	GAANNNNTTC	4 cut(s)	93, 399, 1218, 1668
XspI	CTAG	7 cut(s)	468, 750, 1094, 1170, 3306, 3446, 3558
Zsp2I	ATGCAT	2 cut(s)	1274, 2589

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.