Rorug02G0063900
NAC Family

GPI-GlcNAc transferase complex, PIG-H component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011274
Physical Location & Seq
Forward (+)
5001358 .. 5006689
5332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0063900.1

Sequence Viewer

Length: 3039 bp
ATGACTACACCAACCCCAAAACCCTCTCTCATCCTCCTCATCTTCTTCGTCTTCTTCATCCATGCAAACTCTCAGTCCCTTCAAGACAAGGAATTATCAGTCCTGCTTAAATTAAAGTCATATTGGCTACCTCCACGAGCTTTCAGTCATTGGACTTCATCATCAAACTCTAGTTCCCACTGTAACTGGCCTGAGATCAACTGCACCGGAAACTCCGTCACCGGATTGAATCTCAACAGCGTAAACCTCACCCTTGAAGTTCCACCCTTCATTTGTGACCTCAACAACCTCACACACCTTGACCTTGGTAATAACTCCCTCCCGAGAGGTTTTCCTTTGTCTCTCTATAACTGCTCCAAGCTAGAGTATCTAGACCTCTCGCAAAACTACTTTGTGGGTACAATTCCTAATGACATCGACAGATTGCCTAAGCTTAAGATCCTCAACCTTGCTGGCAACAACTTCTCCGGTGACATTCCGGCGTCTATTGGGAACTTACAGGAGCTGGCTACTTTGGATATATACATGAACCAGCTCAACGGCTCTGTCCCTGCTGAAATAGGTGACCTCTCCAATCTTGAGCAACTCTGGCTCTCTTGGCTTCCCAAAATGGCGCCATGGAAGCTACCTTTTAACTTCACCAAGTTGAAAAAGATGAAGATATTGAAGATACGCGAAGCAAATTTGATTGGGGAAATACCAGAATCTATTGGAGAATTGGAAGCACTTGAAGTATTGGATTTGGCGTGGAACAAGTTGAGCGGGAATATACCAAGCGGTGTGTTTCTGTTGAAGACTTTGAGCATTATATATCTGTTCAAGAACAGGCTTTCAGGGGAGATTCCTCGAGTGGTTGAAGCACTCAACTTGAGCATCATTGACATCTCCGAAAACAATCTCACAGGGCCGATACCAGAAGGTTTCGGAAGCCTTACCAAGTTAACAGATATGAGTTTGTTCGAAAATCATATTTCAGGTGAGATTCCAGAGAGCATTGGCCGCCTGCCAAATCTTGTGATTTTCAAAATGTTCAACATGAACCTGTCAGGAACAATTCCTCCAGAGCTTGGAAAGCATTCGCTGCTCGAAGATTTCCAGGTTTCAGTGAATAGGCTTACCGGAAAGCTGCCAGACGGCTTGTGTAAAAATGGTAAGCTTGTGGCAGTTGTTGCTTATGAGAATAGTCTCACTGGGGAGCTACCAAGCTCTCTTGGAAATTGTGATAGTCTGCTGATAGTCAATGTCTATGATAACATGCTATCTGGGGACATTCCTAGTGGTATGTGGAATGCATTGAATTTGAGTTATGTGATGATGAGCAACAATTCGTTCACGGGTGAGCTTCCTGAGAAGATGTCATATAATCTTCAAAGGCTGGAAATCGGAGATAACAAGTTTTCAGGTCAGATTCCAAGAAGGGTGTCTTCCTGTAAGAATCTGCAAGTTTTTGATGCTGGTAACAACCTCTTCAATGGTAGTATTCCTCAGGAATTAACTACTCTTCGGAGCTTGACGACTCTGACCCTGCATCAGAATCAGCTTAGCGGGCCACTTCCATCAGATTATGCAATGTCACTAAACTCTCTTGATCTGCGTAGAAATCAACTCTCCGGACCAATTCCAGAGATACTTGGTGGTTTGCCCGCCCTTACTGTGTTGGACCTTTCTGAAAACCAGTTTTCAGGTCAAATTCCAAATCAACTTGGCCATCTGAAACTCAACGATGAGTTCAACCTCTCTTCAAATCTCCTCACTGGCGAGATCCCAAATTCATTTGAAAACTCTGCATATGATACAAGCTTCTTGAACAATACCGATCTTTGTGCAAGAACCAGTGTTGTGAATCTCAATATATGCTCTCAATCGCCAAATTCCAGCAAGATTTCAACTCGGTCCCTTGCTTTGATCTTAAGTCTAAGCGCGGTGTTGTTCGTGTTGGTTTTGTCCTTATTAATCTTCTTTGTTAGAGGTTGCTGGAGGAAGGATGGATCAGACGCAGACTGGAAGCTCGTTGCATTTCAGAGGTTGAATTTCACCATCTCACAAATCTTGTCAGGGTTGACAGAAAGCAATGTGATTGGAAGTGGTGGATCAGGGAGGGTCTATCGTGTACCTGTGAATCGTACAGGTGATTTTGTAGCTGCGAAGAGGATTTGGACCAACAAGAAGATCATGGTGGAGAGGCTTGAAAAAGAGTTTCTTGCAGAAGTCGAGATACTGAGCTCAATTCGACATGCTAACATAGTGAAGCTAATGTGCTGCATATCCAGTGAGACTTCGAAACTTCTTGTATATGAGTACTTAGACAATCGAAGCCTAGATCGATGGTTGCACAAGAAAAACAGGCCATACCTATCCAACCTCTCAAGTTCAGTCCATCATGTTGTTCTGGACTGGCCTAAGAGGTTGCAGATTGCAGTGGGAGCTGCTAAGGGCCTCTGCTATATGCATCATGACTGCGTTCCACCCGTTGTGCATCGAGACGTGAAATCAAGTAACATTTTATTGGACTCTGATTTCAACGCAAAAATAGCAGATTTTGGTCTGGCCAAGATGTTGGTCAAGCAAGGAGACCTTGCCACAATGTCAGCTGTTGCTGGTTCCTTTGGCTACATGGCTCCAGAATATGCACATACCAGAAGAGTGAATGAAAAGATCGATGTATATAGTTTCGGGGTCATCCTGCTGGAGTTGGCAACTGGTAGGGAACCTAATGAAGGTGATGAAAACACTTCTCTCGCCGAATGGGCGTGGCGTCGTGCTCAAGAAGGCAAACCTATCGCCAGTGCTCTGGACCAGGACGTCAAAGAACCCTGTTACTTGGATGAAATGTGCGCTGTTTTCAGACTCGGCATCATGTGCACCGAGACACTTCCTACTAATAGGCCTTCTATGAAGGACGTTCTGCAGATCTTGGTTCGAACTTGTCCTCCACCAGTTCGCAGGGAAAAGACTGAATATGCCGCTGCTCCTCTCCTCAAGAACTCGAGGCGGGAGAGAGTTTTGGAAGATCAAGATGGTTTGGCAACGAATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-H

Protein Analysis

1012

Amino Acids

112.33

Weight (kDa)

6.05

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 96 - 155 2.9e-08 Leucine rich repeat
LRR_14 PF23598 115 - 225 2.3e-10 Leucine-rich repeat region
LRR_4 PF12799 120 - 160 9.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 214 - 276 2.4e-06 Leucine-rich repeat region
LRR_8 PF13855 525 - 584 1e-05 Leucine rich repeat
Pkinase PF00069 689 - 970 4.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 690 - 971 2.7e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011274)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35530
fragaria_vesca FvH4_1g10271
malus_domestica MD02G1114800.v1.1 MD15G1230700.v1.1
prunus_persica Prupe.7G183400_v2.0.a1
pyrus_communis pycom02g08970 pycom15g20560
rosa_chinensis RchiOBHm_Chr2g0097101
rosa_laevigata RLG00000016703
rosa_multiflora Rmu_sc0024596.1_g000005
rosa_roxburghii Rroxscaffold_2G00144970
rosa_rugosa Rorug02G0063800 Rorug02G0063900 Rorug02G0064000 Rorug02G0064100
rosa_samantha Rh2AG111300 Rh2BG113200 Rh2CG115400 Rh2DG114600
rosa_wichuraiana Rw2G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2801
AatII GACGTC 1 cut(s) 2805
AccB1I GGYRCC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 1067
AccBSI CCGCTC 1 cut(s) 762
AccII CGCG 2 cut(s) 675, 1922
AccIII TCCGGA 1 cut(s) 1610
AciI CCGC 8 cut(s) 762, 777, 1000, 1545, 1644, 1922, 2964, 2992
AclWI GGATC 4 cut(s) 433, 1756, 1996, 2098
AcoI YGGCCR 3 cut(s) 997, 1705, 2547
AcsI RAATTY 6 cut(s) 682, 1297, 1689, 1768, 1870, 2029
AcyI GRCGYC 4 cut(s) 482, 614, 2755, 2802
AfaI GTAC 4 cut(s) 400, 2112, 2125, 2300
AfiI CCNNNNNNNGG 2 cut(s) 1067, 2717
AflII CTTAAG 2 cut(s) 434, 1909
AjiI CACGTC 1 cut(s) 2485
AjnI CCWGG 2 cut(s) 1095, 2796
AloI GAACNNNNNNTCC 4 cut(s) 1042, 1074, 2914, 2946
Alw21I GWGCWC 4 cut(s) 2225, 2764, 2791, 2864
Alw26I GTCTC 6 cut(s) 345, 1190, 2267, 2475, 2565, 2861
Alw44I GTGCAC 1 cut(s) 2860
AlwI GGATC 4 cut(s) 433, 1756, 1996, 2098
AlwNI CAGNNNCTG 1 cut(s) 505
Ama87I CYCGRG 3 cut(s) 322, 846, 2986
Aor13HI TCCGGA 1 cut(s) 1610
ApaLI GTGCAC 1 cut(s) 2860
ApeKI GCWGC 6 cut(s) 1081, 1126, 2141, 2259, 2426, 2966
ApoI RAATTY 6 cut(s) 682, 1297, 1689, 1768, 1870, 2029
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AseI ATTAAT 1 cut(s) 1952
Asp700I GAANNNNTTC 1 cut(s) 1075
AspLEI GCGC 3 cut(s) 616, 1922, 2837
AspS9I GGNCC 8 cut(s) 905, 1547, 1613, 1660, 1893, 2157, 2434, 2794
AsuII TTCGAA 3 cut(s) 960, 2279, 2920
AvaI CYCGRG 3 cut(s) 322, 846, 2986
AvaII GGWCC 5 cut(s) 1613, 1660, 1893, 2157, 2794
AxyI CCTNAGG 1 cut(s) 1485
BaeGI GKGCMC 1 cut(s) 2864
BalI TGGCCA 2 cut(s) 1707, 2549
BanI GGYRCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 2225
BarI GAAGNNNNNNTAC 2 cut(s) 2199, 2231
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 3 cut(s) 43, 800, 1416
Bbv12I GWGCWC 4 cut(s) 2225, 2764, 2791, 2864
BbvI GCAGC 6 cut(s) 1068, 1113, 2128, 2246, 2413, 2953
BccI CCATC 7 cut(s) 1564, 1716, 1979, 2045, 2319, 2385, 3011
BceAI ACGGC 2 cut(s) 556, 1150
BcgI CGANNNNNNTGC 4 cut(s) 1805, 1839, 2761, 2795
BciT130I CCWGG 2 cut(s) 1097, 2798
BcoDI GTCTC 6 cut(s) 345, 1190, 2267, 2475, 2565, 2861
BfaI CTAG 5 cut(s) 171, 362, 371, 1275, 2318
BfmI CTRYAG 1 cut(s) 2906
BfoI RGCGCY 1 cut(s) 617
BfrI CTTAAG 2 cut(s) 434, 1909
BglI GCCNNNNNGGC 1 cut(s) 2747
BglII AGATCT 1 cut(s) 2910
BisI GCNGC 8 cut(s) 1000, 1082, 1127, 2142, 2260, 2427, 2964, 2967
BlpI GCTNAGC 1 cut(s) 1541
BlsI GCNGC 8 cut(s) 1001, 1083, 1128, 2143, 2261, 2428, 2965, 2968
BmcAI AGTACT 1 cut(s) 2300
Bme1390I CCNGG 2 cut(s) 1097, 2798
Bme18I GGWCC 5 cut(s) 1613, 1660, 1893, 2157, 2794
BmeT110I CYCGRG 3 cut(s) 322, 846, 2986
BmgBI CACGTC 1 cut(s) 2485
BmgT120I GGNCC 8 cut(s) 905, 1547, 1613, 1660, 1893, 2157, 2434, 2794
BmiI GGNNCC 5 cut(s) 615, 1895, 2602, 2619, 2709
BmrFI CCNGG 2 cut(s) 1097, 2798
BmrI ACTGGG 1 cut(s) 1200
BmsI GCATC 6 cut(s) 882, 1441, 1537, 2458, 2485, 2862
BmuI ACTGGG 1 cut(s) 1200
BpiI GAAGAC 3 cut(s) 43, 800, 1416
BpmI CTGGAG 4 cut(s) 1044, 1996, 2604, 2708
Bpu10I CCTNAGC 2 cut(s) 429, 2430
Bpu1102I GCTNAGC 1 cut(s) 1541
Bpu14I TTCGAA 3 cut(s) 960, 2279, 2920
BpuEI CTTGAG 5 cut(s) 599, 889, 2350, 2748, 2963
Bsa29I ATCGAT 2 cut(s) 2323, 2658
BsaHI GRCGYC 4 cut(s) 482, 614, 2755, 2802
BsaI GGTCTC 1 cut(s) 2565
BsaJI CCNNGG 2 cut(s) 304, 617
BsaWI WCCGGW 5 cut(s) 206, 221, 467, 1118, 1610
BsaXI ACNNNNNCTCC 6 cut(s) 449, 479, 1042, 1072, 2914, 2944
Bsc4I CCNNNNNNNGG 2 cut(s) 1067, 2717
Bse21I CCTNAGG 1 cut(s) 1485
Bse3DI GCAATG 2 cut(s) 1575, 2077
BseAI TCCGGA 1 cut(s) 1610
BseBI CCWGG 2 cut(s) 1097, 2798
BseCI ATCGAT 2 cut(s) 2323, 2658
BseDI CCNNGG 2 cut(s) 304, 617
BseGI GGATG 5 cut(s) 30, 57, 1990, 2679, 2830
BseLI CCNNNNNNNGG 2 cut(s) 1067, 2717
BseMI GCAATG 2 cut(s) 1575, 2077
BseMII CTCAG 5 cut(s) 86, 183, 1338, 1499, 2210
BseRI GAGGAG 4 cut(s) 26, 1739, 2961, 2966
BseSI GKGCMC 1 cut(s) 2864
BseXI GCAGC 6 cut(s) 1068, 1113, 2128, 2246, 2413, 2953
BsgI GTGCAG 1 cut(s) 187
Bsh1236I CGCG 2 cut(s) 675, 1922
BshNI GGYRCC 1 cut(s) 613
BshVI ATCGAT 2 cut(s) 2323, 2658
BsiHKAI GWGCWC 4 cut(s) 2225, 2764, 2791, 2864
BsiHKCI CYCGRG 3 cut(s) 322, 846, 2986
BsiSI CCGG 6 cut(s) 207, 222, 468, 479, 1119, 1611
BslFI GGGAC 4 cut(s) 61, 533, 1280, 1879
BslI CCNNNNNNNGG 2 cut(s) 1067, 2717
BsmAI GTCTC 6 cut(s) 345, 1190, 2267, 2475, 2565, 2861
BsmBI CGTCTC 1 cut(s) 2475
BsmFI GGGAC 4 cut(s) 61, 533, 1280, 1879
BsmI GAATGC 2 cut(s) 1075, 1294
Bso31I GGTCTC 1 cut(s) 2565
BsoBI CYCGRG 3 cut(s) 322, 846, 2986
Bsp119I TTCGAA 3 cut(s) 960, 2279, 2920
Bsp1286I GDGCHC 4 cut(s) 2225, 2764, 2791, 2864
Bsp13I TCCGGA 1 cut(s) 1610
Bsp1720I GCTNAGC 1 cut(s) 1541
Bsp19I CCATGG 1 cut(s) 617
BspACI CCGC 8 cut(s) 762, 777, 1000, 1545, 1644, 1922, 2964, 2992
BspCNI CTCAG 5 cut(s) 85, 184, 1339, 1498, 2211
BspDI ATCGAT 2 cut(s) 2323, 2658
BspEI TCCGGA 1 cut(s) 1610
BspFNI CGCG 2 cut(s) 675, 1922
BspHI TCATGA 1 cut(s) 2452
BspLI GGNNCC 5 cut(s) 615, 1895, 2602, 2619, 2709
BspMAI CTGCAG 1 cut(s) 2910
BspPI GGATC 4 cut(s) 433, 1756, 1996, 2098
BspT104I TTCGAA 3 cut(s) 960, 2279, 2920
BspT107I GGYRCC 1 cut(s) 613
BspTI CTTAAG 2 cut(s) 434, 1909
BspTNI GGTCTC 1 cut(s) 2565
BsrBI CCGCTC 1 cut(s) 762
BsrDI GCAATG 2 cut(s) 1575, 2077
BssECI CCNNGG 2 cut(s) 304, 617
BssNI GRCGYC 4 cut(s) 482, 614, 2755, 2802
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 304, 617
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 1097, 2798
Bst4CI ACNGT 2 cut(s) 182, 1654
Bst6I CTCTTC 5 cut(s) 1472, 1506, 1744, 2141, 2635
BstACI GRCGYC 4 cut(s) 482, 614, 2755, 2802
BstAFI CTTAAG 2 cut(s) 434, 1909
BstAPI GCANNNNNTGC 3 cut(s) 1081, 1169, 2859
BstBI TTCGAA 3 cut(s) 960, 2279, 2920
BstC8I GCNNGC 5 cut(s) 454, 507, 1004, 1547, 1644
BstDSI CCRYGG 1 cut(s) 617
BstEII GGTNACC 1 cut(s) 563
BstENI CCTNNNNNAGG 1 cut(s) 2715
BstF5I GGATG 5 cut(s) 30, 57, 1990, 2679, 2830
BstFNI CGCG 2 cut(s) 675, 1922
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 3 cut(s) 616, 1922, 2837
BstMAI GTCTC 6 cut(s) 345, 1190, 2267, 2475, 2565, 2861
BstNI CCWGG 2 cut(s) 1097, 2798
BstNSI RCATGY 2 cut(s) 1258, 2237
BstPI GGTNACC 1 cut(s) 563
BstSCI CCNGG 2 cut(s) 1095, 2796
BstSFI CTRYAG 1 cut(s) 2906
BstSLI GKGCMC 1 cut(s) 2864
BstUI CGCG 2 cut(s) 675, 1922
BstV1I GCAGC 6 cut(s) 1068, 1113, 2128, 2246, 2413, 2953
BstV2I GAAGAC 3 cut(s) 43, 800, 1416
BstX2I RGATCY 3 cut(s) 438, 1761, 2910
BstXI CCANNNNNNTGG 2 cut(s) 2557, 2791
BstYI RGATCY 3 cut(s) 438, 1761, 2910
Bsu15I ATCGAT 2 cut(s) 2323, 2658
Bsu36I CCTNAGG 1 cut(s) 1485
BsuTUI ATCGAT 2 cut(s) 2323, 2658
BtgI CCRYGG 1 cut(s) 617
BtrI CACGTC 1 cut(s) 2485
BtsCI GGATG 5 cut(s) 30, 57, 1990, 2679, 2830
BtsI GCAGTG 1 cut(s) 2424
BtsIMutI CAGTG 8 cut(s) 178, 1110, 1188, 1752, 1840, 2275, 2424, 2791
Cac8I GCNNGC 5 cut(s) 454, 507, 1004, 1547, 1644
CaiI CAGNNNCTG 1 cut(s) 505
CciI TCATGA 1 cut(s) 2452
CfoI GCGC 3 cut(s) 616, 1922, 2837
Cfr13I GGNCC 8 cut(s) 905, 1547, 1613, 1660, 1893, 2157, 2434, 2794
ClaI ATCGAT 2 cut(s) 2323, 2658
CseI GACGC 3 cut(s) 471, 2003, 2744
Csp6I GTAC 4 cut(s) 399, 2111, 2124, 2299
CviQI GTAC 4 cut(s) 399, 2111, 2124, 2299
DinI GGCGCC 1 cut(s) 615
DrdI GACNNNNNNGTC 1 cut(s) 2801
DseDI GACNNNNNNGTC 1 cut(s) 2801
EaeI YGGCCR 3 cut(s) 997, 1705, 2547
Eam1104I CTCTTC 5 cut(s) 1472, 1506, 1744, 2141, 2635
EarI CTCTTC 5 cut(s) 1472, 1506, 1744, 2141, 2635
Ecl136II GAGCTC 1 cut(s) 2223
Eco130I CCWWGG 2 cut(s) 304, 617
Eco147I AGGCCT 1 cut(s) 2887
Eco24I GRGCYC 1 cut(s) 2225
Eco31I GGTCTC 1 cut(s) 2565
Eco47I GGWCC 5 cut(s) 1613, 1660, 1893, 2157, 2794
Eco53kI GAGCTC 1 cut(s) 2223
Eco81I CCTNAGG 1 cut(s) 1485
Eco88I CYCGRG 3 cut(s) 322, 846, 2986
Eco91I GGTNACC 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 2223
EcoNI CCTNNNNNAGG 1 cut(s) 2715
EcoO109I RGGNCCY 1 cut(s) 2434
EcoO65I GGTNACC 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 1095, 2796
EcoT14I CCWWGG 2 cut(s) 304, 617
EcoT22I ATGCAT 2 cut(s) 1294, 2451
EcoT38I GRGCYC 1 cut(s) 2225
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
ErhI CCWWGG 2 cut(s) 304, 617
Esp3I CGTCTC 1 cut(s) 2475
FalI AAGNNNNNCTT 6 cut(s) 1408, 1440, 2184, 2216, 2559, 2591
FaqI GGGAC 4 cut(s) 61, 533, 1280, 1879
FauI CCCGC 4 cut(s) 755, 1538, 1651, 2985
FauNDI CATATG 1 cut(s) 1789
Fnu4HI GCNGC 8 cut(s) 1000, 1082, 1127, 2142, 2260, 2427, 2964, 2967
FokI GGATG 5 cut(s) 17, 44, 1997, 2666, 2837
FriOI GRGCYC 1 cut(s) 2225
Fsp4HI GCNGC 8 cut(s) 1000, 1082, 1127, 2142, 2260, 2427, 2964, 2967
FspBI CTAG 5 cut(s) 171, 362, 371, 1275, 2318
GlaI GCGC 3 cut(s) 615, 1921, 2836
GluI GCNGC 8 cut(s) 1000, 1082, 1127, 2142, 2260, 2427, 2964, 2967
GsuI CTGGAG 4 cut(s) 1044, 1996, 2604, 2708
HaeII RGCGCY 1 cut(s) 617
HapII CCGG 6 cut(s) 207, 222, 468, 479, 1119, 1611
HgaI GACGC 3 cut(s) 471, 2003, 2744
HhaI GCGC 3 cut(s) 616, 1922, 2837
Hin1I GRCGYC 4 cut(s) 482, 614, 2755, 2802
Hin6I GCGC 3 cut(s) 614, 1920, 2835
HinP1I GCGC 3 cut(s) 614, 1920, 2835
HincII GTYRAC 2 cut(s) 942, 2061
HindII GTYRAC 2 cut(s) 942, 2061
HindIII AAGCTT 3 cut(s) 431, 1154, 1798
HpaI GTTAAC 1 cut(s) 942
HpaII CCGG 6 cut(s) 207, 222, 468, 479, 1119, 1611
Hpy166II GTNNAC 6 cut(s) 244, 942, 1332, 2061, 2111, 2862
Hpy8I GTNNAC 6 cut(s) 244, 942, 1332, 2061, 2111, 2862
Hpy99I CGWCG 1 cut(s) 2760
HpyAV CCTTC 9 cut(s) 89, 277, 911, 1410, 1975, 2711, 2762, 2890, 2898
HpyCH4III ACNGT 2 cut(s) 182, 1654
HpyCH4IV ACGT 3 cut(s) 2484, 2802, 2901
HpySE526I ACGT 3 cut(s) 2484, 2802, 2901
Hsp92I GRCGYC 4 cut(s) 482, 614, 2755, 2802
HspAI GCGC 3 cut(s) 614, 1920, 2835
KasI GGCGCC 1 cut(s) 613
Kpn2I TCCGGA 1 cut(s) 1610
KspAI GTTAAC 1 cut(s) 942
LmnI GCTCC 7 cut(s) 359, 502, 1195, 1506, 2423, 2623, 2974
Lsp1109I GCAGC 6 cut(s) 1068, 1113, 2128, 2246, 2413, 2953
LweI GCATC 6 cut(s) 882, 1441, 1537, 2458, 2485, 2862
MaeI CTAG 5 cut(s) 171, 362, 371, 1275, 2318
MaeII ACGT 3 cut(s) 2484, 2802, 2901
MaeIII GTNAC 9 cut(s) 182, 217, 275, 470, 563, 1457, 1572, 2495, 2816
MbiI CCGCTC 1 cut(s) 762
MflI RGATCY 3 cut(s) 438, 1761, 2910
MhlI GDGCHC 4 cut(s) 2225, 2764, 2791, 2864
MlsI TGGCCA 2 cut(s) 1707, 2549
MluNI TGGCCA 2 cut(s) 1707, 2549
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 3 cut(s) 1510, 2504, 2841
MmeI TCCRAC 2 cut(s) 1638, 2382
Mox20I TGGCCA 2 cut(s) 1707, 2549
Mph1103I ATGCAT 2 cut(s) 1294, 2451
MroI TCCGGA 1 cut(s) 1610
MroXI GAANNNNTTC 1 cut(s) 1075
MscI TGGCCA 2 cut(s) 1707, 2549
MseI TTAA 8 cut(s) 108, 113, 435, 633, 941, 1493, 1910, 1952
Msp20I TGGCCA 2 cut(s) 1707, 2549
MspA1I CMGCKG 2 cut(s) 2591, 2966
MspCI CTTAAG 2 cut(s) 434, 1909
MspI CCGG 6 cut(s) 207, 222, 468, 479, 1119, 1611
MspR9I CCNGG 2 cut(s) 1097, 2798
Mva1269I GAATGC 2 cut(s) 1075, 1294
MvaI CCWGG 2 cut(s) 1097, 2798
MvnI CGCG 2 cut(s) 675, 1922
NarI GGCGCC 1 cut(s) 614
NcoI CCATGG 1 cut(s) 617
NdeI CATATG 1 cut(s) 1789
NlaIV GGNNCC 5 cut(s) 615, 1895, 2602, 2619, 2709
NmeAIII GCCGAG 1 cut(s) 2829
NmuCI GTSAC 5 cut(s) 217, 275, 470, 563, 1572
NsiI ATGCAT 2 cut(s) 1294, 2451
NspI RCATGY 2 cut(s) 1258, 2237
NspV TTCGAA 3 cut(s) 960, 2279, 2920
PaeR7I CTCGAG 2 cut(s) 846, 2986
PagI TCATGA 1 cut(s) 2452
PceI AGGCCT 1 cut(s) 2887
PctI GAATGC 2 cut(s) 1075, 1294
PdmI GAANNNNTTC 1 cut(s) 1075
PfeI GAWTC 9 cut(s) 229, 704, 841, 982, 1408, 1435, 1534, 1843, 2119
PflMI CCANNNNNTGG 1 cut(s) 1067
PkrI GCNGC 8 cut(s) 1001, 1083, 1128, 2143, 2261, 2428, 2965, 2968
PleI GAGTC 3 cut(s) 1510, 2504, 2841
PluTI GGCGCC 1 cut(s) 617
PpsI GAGTC 3 cut(s) 1510, 2504, 2841
PshBI ATTAAT 1 cut(s) 1952
Psp124BI GAGCTC 1 cut(s) 2225
Psp6I CCWGG 2 cut(s) 1095, 2796
PspEI GGTNACC 1 cut(s) 563
PspGI CCWGG 2 cut(s) 1095, 2796
PspN4I GGNNCC 5 cut(s) 615, 1895, 2602, 2619, 2709
PspPI GGNCC 8 cut(s) 905, 1547, 1613, 1660, 1893, 2157, 2434, 2794
PspXI VCTCGAGB 2 cut(s) 846, 2986
PstI CTGCAG 1 cut(s) 2910
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 3 cut(s) 438, 1761, 2910
PvuII CAGCTG 1 cut(s) 2591
RsaI GTAC 4 cut(s) 400, 2112, 2125, 2300
RsaNI GTAC 4 cut(s) 399, 2111, 2124, 2299
SacI GAGCTC 1 cut(s) 2225
SaqAI TTAA 8 cut(s) 108, 113, 435, 633, 941, 1493, 1910, 1952
SatI GCNGC 8 cut(s) 1000, 1082, 1127, 2142, 2260, 2427, 2964, 2967
Sau96I GGNCC 8 cut(s) 905, 1547, 1613, 1660, 1893, 2157, 2434, 2794
ScaI AGTACT 1 cut(s) 2300
SchI GAGTC 3 cut(s) 1510, 2504, 2841
ScrFI CCNGG 2 cut(s) 1097, 2798
SduI GDGCHC 4 cut(s) 2225, 2764, 2791, 2864
SfaNI GCATC 6 cut(s) 882, 1441, 1537, 2458, 2485, 2862
SfcI CTRYAG 1 cut(s) 2906
SfoI GGCGCC 1 cut(s) 615
Sfr274I CTCGAG 2 cut(s) 846, 2986
SfuI TTCGAA 3 cut(s) 960, 2279, 2920
SinI GGWCC 5 cut(s) 1613, 1660, 1893, 2157, 2794
SlaI CTCGAG 2 cut(s) 846, 2986
SmlI CTYRAG 9 cut(s) 434, 578, 846, 868, 1909, 2365, 2763, 2978, 2986
SmoI CTYRAG 9 cut(s) 434, 578, 846, 868, 1909, 2365, 2763, 2978, 2986
SseBI AGGCCT 1 cut(s) 2887
SsiI CCGC 8 cut(s) 762, 777, 1000, 1545, 1644, 1922, 2964, 2992
SspDI GGCGCC 1 cut(s) 613
SspMI CTAG 5 cut(s) 171, 362, 371, 1275, 2318
SstI GAGCTC 1 cut(s) 2225
StuI AGGCCT 1 cut(s) 2887
StyD4I CCNGG 2 cut(s) 1095, 2796
StyI CCWWGG 2 cut(s) 304, 617
TaaI ACNGT 2 cut(s) 182, 1654
TaiI ACGT 3 cut(s) 2487, 2805, 2904
TaqII GACCGA 1 cut(s) 1881
TatI WGTACW 1 cut(s) 2298
TauI GCSGC 2 cut(s) 1002, 2966
TfiI GAWTC 9 cut(s) 229, 704, 841, 982, 1408, 1435, 1534, 1843, 2119
Tru1I TTAA 8 cut(s) 108, 113, 435, 633, 941, 1493, 1910, 1952
Tru9I TTAA 8 cut(s) 108, 113, 435, 633, 941, 1493, 1910, 1952
TscAI CASTG 8 cut(s) 185, 1110, 1195, 1759, 1840, 2275, 2424, 2791
TseFI GTSAC 5 cut(s) 217, 275, 470, 563, 1572
TseI GCWGC 6 cut(s) 1081, 1126, 2141, 2259, 2426, 2966
Tsp45I GTSAC 5 cut(s) 217, 275, 470, 563, 1572
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 8 cut(s) 185, 1110, 1195, 1759, 1840, 2275, 2424, 2791
Van91I CCANNNNNTGG 1 cut(s) 1067
Vha464I CTTAAG 2 cut(s) 434, 1909
VneI GTGCAC 1 cut(s) 2860
VpaK11BI GGWCC 5 cut(s) 1613, 1660, 1893, 2157, 2794
VspI ATTAAT 1 cut(s) 1952
XagI CCTNNNNNAGG 1 cut(s) 2715
XapI RAATTY 6 cut(s) 682, 1297, 1689, 1768, 1870, 2029
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 2 cut(s) 1258, 2237
XhoI CTCGAG 2 cut(s) 846, 2986
XmnI GAANNNNTTC 1 cut(s) 1075
XspI CTAG 5 cut(s) 171, 362, 371, 1275, 2318
ZraI GACGTC 1 cut(s) 2803
ZrmI AGTACT 1 cut(s) 2300
Zsp2I ATGCAT 2 cut(s) 1294, 2451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.