Rorug02G0137300

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009595
Physical Location & Seq
Forward (+)
12105200 .. 12106864
1665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0137300.1

Sequence Viewer

Length: 1494 bp
ATGTATCCTCTGAATCTCAGAGAGATGCCCACAACAGCATCGTCCTTGTTCTCCGCCTATGCCTCCTTCACTGCACTCATCATGTTGGTCAAGTCCATGGCGGACCAACTCATGTCACTCATCCCTCCTCAAGTCCGCTCATACTTGTCCTCAATCCTCCACCACTTCTATGCCCCTCGCTCCGCTGACCTCACTCTCATCGTAGACGAGAAGTGTGGCCATATGATAAACCAAGTCTATGAAGCCGTGGAGGTCTACCTCAAGACCAAGATCAGTCCACTCAACGAGCGTCTGCGAGTGAGCAGAACACCCGGACAGAAAACTCTCAGCATTACCATCGACAGAAACGAAGAGATCATCGACTACTTCGACAACATCACACTAAAGTGGCGTTATGTGTGCACTGAAGCACAAAAAGGTGGGGGTGGACCGGGTGGTGACAAGGAAAAGCGCAAGTTTGAGCTTACTTTCAACAAGAAACACAAGGGCGAGGTGATAGACTCGTACCTACACAATGTGTTGGCTCGGGCTGATGCACTTAGACAAGAGGAAAAGGTCCTCAAGCTTGCTTCCCAAAATTCGGGTAGTTCCATAGATCTCCAGCACCCTTCAACTTTTGACACACTGGCTATGGACCCCAAGCTCAAGAGAATGATTATCGAGGATTTGGACAGGTTCTTGAGGAGGAAGGAGTTTTACAAGAAGGTTGGCAAGGCTTGGAAAAGAGGTTACTTGTTGTATGGTCCACCTGGTACTGGAAAATCAAGCTTGATTGCAGCCATGGCTAATTATCTCAAGTTTGATGTCCATGATTTGGACCTCAGTAGAATTTTCAACAACAGTGAATTGAGGAGGATTCTGCTGTCTTCTTCAAATCGTTCTATTTTGGTCATTGAGGACATTGATTGCAGCGTGCAAATAGAGAACCGGAAAGAGGAGAAAAGTCGACAATCTAATACCAATTTTACTCTCTCGGGCCTACTGAACTTCATAGATGGTCTGTGGTCAAGCAGCGGTGATGGGAGAATCATTGTGTTTACCACCAACCATAAGGACAAACTAGACCCTGCATTGTTGCGTCCCGGTCGAATGGATGTGCACATTCACATGTCGTATTGCACTGCGAGCGGGTTCAGAATCTTGGCCTCTAACTACCTCGGCATTCAAGAAAGCAACCCTCATAGCCTTTGTGGAGAAATTGAAGGCTTAATAGAGAGCACTGAGGTAACCCCTGCAGAGGTTGCTGAGGAGCTCTTGAAGAGTGATGATGCTGATGTTGTTCTTGAAGGACTTGTTGATTTCCTGAAAAGAAAGAAGGAAGAGAACAATAAAATTGATGATGAAGGAACTCAAGAAGCTGATCAAGATGCAGAAAAAGGGATCTCATCCGGTTGCGATAACTGCAATGATGGAAGTGGCGAAGAATGCGACGATGGAAGTGGCGAAGAATGCGACGATGGAAGTGGTTCAGAATCTTCTCTTGAAAATAATTGA

Protein Analysis

497

Amino Acids

55.64

Weight (kDa)

5.25

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 41 - 134 1.4e-22 Domain associated at C-terminal with AAA
AAA PF00004 244 - 371 3.9e-22 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 373 - 444 8.1e-22 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 814
AccBSI CCGCTC 2 cut(s) 138, 1128
AccI GTMKAC 3 cut(s) 204, 255, 946
AciI CCGC 6 cut(s) 54, 101, 136, 183, 1014, 1128
AclWI GGATC 1 cut(s) 1388
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 2 cut(s) 577, 828
AcuI CTGAAG 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 517
AfaI GTAC 2 cut(s) 506, 754
AfiI CCNNNNNNNGG 5 cut(s) 580, 755, 814, 934, 1237
AflIII ACRYGT 1 cut(s) 1107
AgsI TTSAA 9 cut(s) 472, 612, 835, 873, 1166, 1202, 1258, 1286, 1484
AjnI CCWGG 1 cut(s) 748
AleI CACNNNNGTG 1 cut(s) 385
AluBI AGCT 6 cut(s) 463, 565, 643, 768, 1252, 1358
AluI AGCT 6 cut(s) 463, 565, 643, 768, 1252, 1358
Alw21I GWGCWC 4 cut(s) 404, 1101, 1220, 1254
Alw44I GTGCAC 2 cut(s) 400, 1097
AlwI GGATC 1 cut(s) 1388
Ama87I CYCGRG 2 cut(s) 525, 973
AoxI GGCC 3 cut(s) 217, 976, 1143
ApaLI GTGCAC 2 cut(s) 400, 1097
ApeKI GCWGC 3 cut(s) 776, 909, 1011
ApoI RAATTY 2 cut(s) 577, 828
Asp700I GAANNNNTTC 1 cut(s) 1465
AspLEI GCGC 1 cut(s) 453
AspS9I GGNCC 7 cut(s) 103, 428, 556, 634, 743, 817, 976
AsuC2I CCSGG 3 cut(s) 312, 432, 1083
AsuHPI GGTGA 3 cut(s) 449, 505, 1028
AvaI CYCGRG 2 cut(s) 525, 973
AvaII GGWCC 6 cut(s) 103, 428, 556, 634, 743, 817
BaeGI GKGCMC 2 cut(s) 404, 1101
BalI TGGCCA 1 cut(s) 219
BanII GRGCYC 1 cut(s) 1254
BarI GAAGNNNNNNTAC 2 cut(s) 680, 712
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 4 cut(s) 404, 1101, 1220, 1254
BbvCI CCTCAGC 1 cut(s) 1245
BbvI GCAGC 3 cut(s) 788, 921, 1023
BccI CCATC 6 cut(s) 344, 989, 1013, 1403, 1427, 1451
BceAI ACGGC 1 cut(s) 230
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 1 cut(s) 750
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 1360
BcnI CCSGG 3 cut(s) 312, 432, 1083
BfaI CTAG 1 cut(s) 1061
BfmI CTRYAG 1 cut(s) 1233
BfuI GTATCC 1 cut(s) 15
BglII AGATCT 1 cut(s) 595
BisI GCNGC 3 cut(s) 777, 910, 1012
BlsI GCNGC 3 cut(s) 778, 911, 1013
Bme1390I CCNGG 4 cut(s) 312, 432, 750, 1083
Bme18I GGWCC 6 cut(s) 103, 428, 556, 634, 743, 817
BmeT110I CYCGRG 2 cut(s) 525, 973
BmgT120I GGNCC 7 cut(s) 103, 428, 556, 634, 743, 817, 976
BmiI GGNNCC 1 cut(s) 636
BmrFI CCNGG 4 cut(s) 312, 432, 750, 1083
BmsI GCATC 5 cut(s) 15, 47, 523, 1258, 1357
BpiI GAAGAC 1 cut(s) 858
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 1245
BpuEI CTTGAG 7 cut(s) 114, 245, 545, 629, 700, 779, 1335
BpuMI CCSGG 3 cut(s) 312, 432, 1083
BsaJI CCNNGG 4 cut(s) 96, 246, 780, 1156
BsaWI WCCGGW 2 cut(s) 927, 1388
Bsc4I CCNNNNNNNGG 5 cut(s) 580, 755, 814, 934, 1237
Bse1I ACTGG 2 cut(s) 630, 760
Bse3DI GCAATG 1 cut(s) 1411
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 4 cut(s) 96, 246, 780, 1156
BseGI GGATG 3 cut(s) 120, 1099, 1385
BseLI CCNNNNNNNGG 5 cut(s) 580, 755, 814, 934, 1237
BseMI GCAATG 1 cut(s) 1411
BseMII CTCAG 5 cut(s) 31, 340, 835, 1212, 1236
BseNI ACTGG 2 cut(s) 630, 760
BseRI GAGGAG 5 cut(s) 117, 697, 865, 950, 1262
BseSI GKGCMC 2 cut(s) 404, 1101
BseXI GCAGC 3 cut(s) 788, 921, 1023
BsgI GTGCAG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 1087
BshFI GGCC 3 cut(s) 219, 978, 1145
BsiEI CGRYCG 1 cut(s) 1087
BsiHKAI GWGCWC 4 cut(s) 404, 1101, 1220, 1254
BsiHKCI CYCGRG 2 cut(s) 525, 973
BsiSI CCGG 5 cut(s) 312, 431, 928, 1083, 1389
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 5 cut(s) 580, 755, 814, 934, 1237
BsmFI GGGAC 1 cut(s) 1065
BsmI GAATGC 3 cut(s) 1161, 1430, 1454
BsnI GGCC 3 cut(s) 219, 978, 1145
BsoBI CYCGRG 2 cut(s) 525, 973
Bsp1286I GDGCHC 4 cut(s) 404, 1101, 1220, 1254
Bsp143I GATC 5 cut(s) 270, 354, 595, 1360, 1380
Bsp19I CCATGG 2 cut(s) 96, 780
BspACI CCGC 6 cut(s) 54, 101, 136, 183, 1014, 1128
BspANI GGCC 3 cut(s) 219, 978, 1145
BspCNI CTCAG 5 cut(s) 30, 339, 834, 1213, 1237
BspLI GGNNCC 1 cut(s) 636
BspMAI CTGCAG 1 cut(s) 1237
BspPI GGATC 1 cut(s) 1388
BsrBI CCGCTC 2 cut(s) 138, 1128
BsrDI GCAATG 1 cut(s) 1411
BsrI ACTGG 2 cut(s) 630, 760
BssECI CCNNGG 4 cut(s) 96, 246, 780, 1156
BssMI GATC 5 cut(s) 270, 354, 595, 1360, 1380
BssT1I CCWWGG 2 cut(s) 96, 780
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 1 cut(s) 842
Bst6I CTCTTC 3 cut(s) 345, 1253, 1314
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstC8I GCNNGC 3 cut(s) 567, 914, 1126
BstDEI CTNAG 6 cut(s) 17, 326, 539, 821, 1221, 1245
BstDSI CCRYGG 3 cut(s) 96, 246, 780
BstEII GGTNACC 1 cut(s) 1225
BstF5I GGATG 3 cut(s) 120, 1099, 1385
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 5 cut(s) 273, 357, 598, 1363, 1383
BstMBI GATC 5 cut(s) 270, 354, 595, 1360, 1380
BstMCI CGRYCG 1 cut(s) 1087
BstMWI GCNNNNNNNGC 6 cut(s) 782, 1125, 1241, 1401, 1425, 1449
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 1 cut(s) 1111
BstPI GGTNACC 1 cut(s) 1225
BstSCI CCNGG 4 cut(s) 310, 430, 748, 1081
BstSFI CTRYAG 1 cut(s) 1233
BstSLI GKGCMC 2 cut(s) 404, 1101
BstV1I GCAGC 3 cut(s) 788, 921, 1023
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 2 cut(s) 595, 1380
BstYI RGATCY 2 cut(s) 595, 1380
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 219, 978, 1145
BtgI CCRYGG 3 cut(s) 96, 246, 780
BtsCI GGATG 3 cut(s) 120, 1099, 1385
BtsI GCAGTG 2 cut(s) 69, 1119
BtsIMutI CAGTG 6 cut(s) 69, 402, 623, 847, 1119, 1218
Cac8I GCNNGC 3 cut(s) 567, 914, 1126
CfoI GCGC 1 cut(s) 453
Cfr13I GGNCC 7 cut(s) 103, 428, 556, 634, 743, 817, 976
CseI GACGC 2 cut(s) 278, 1067
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 2 cut(s) 505, 753
CviAII CATG 6 cut(s) 82, 97, 112, 781, 809, 1108
CviQI GTAC 2 cut(s) 505, 753
DdeI CTNAG 6 cut(s) 17, 326, 539, 821, 1221, 1245
DpnI GATC 5 cut(s) 272, 356, 597, 1362, 1382
DpnII GATC 5 cut(s) 270, 354, 595, 1360, 1380
DraIII CACNNNGTG 1 cut(s) 517
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 3 cut(s) 345, 1253, 1314
EarI CTCTTC 3 cut(s) 345, 1253, 1314
EciI GGCGGA 2 cut(s) 43, 116
Ecl136II GAGCTC 1 cut(s) 1252
Eco130I CCWWGG 2 cut(s) 96, 780
Eco24I GRGCYC 1 cut(s) 1254
Eco47I GGWCC 6 cut(s) 103, 428, 556, 634, 743, 817
Eco53kI GAGCTC 1 cut(s) 1252
Eco57I CTGAAG 1 cut(s) 426
Eco88I CYCGRG 2 cut(s) 525, 973
Eco91I GGTNACC 1 cut(s) 1225
EcoICRI GAGCTC 1 cut(s) 1252
EcoO109I RGGNCCY 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 1225
EcoRII CCWGG 1 cut(s) 748
EcoT14I CCWWGG 2 cut(s) 96, 780
EcoT38I GRGCYC 1 cut(s) 1254
ErhI CCWWGG 2 cut(s) 96, 780
FaeI CATG 6 cut(s) 85, 100, 115, 784, 812, 1111
FaqI GGGAC 1 cut(s) 1065
FatI CATG 6 cut(s) 81, 96, 111, 780, 808, 1107
FauI CCCGC 1 cut(s) 1121
FauNDI CATATG 1 cut(s) 222
FbaI TGATCA 1 cut(s) 1360
FblI GTMKAC 3 cut(s) 204, 255, 946
Fnu4HI GCNGC 3 cut(s) 777, 910, 1012
FokI GGATG 3 cut(s) 107, 1106, 1372
FriOI GRGCYC 1 cut(s) 1254
Fsp4HI GCNGC 3 cut(s) 777, 910, 1012
FspBI CTAG 1 cut(s) 1061
GlaI GCGC 1 cut(s) 452
GluI GCNGC 3 cut(s) 777, 910, 1012
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 3 cut(s) 219, 978, 1145
HapII CCGG 5 cut(s) 312, 431, 928, 1083, 1389
HgaI GACGC 2 cut(s) 278, 1067
HhaI GCGC 1 cut(s) 453
Hin1II CATG 6 cut(s) 85, 100, 115, 784, 812, 1111
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HincII GTYRAC 1 cut(s) 947
HindII GTYRAC 1 cut(s) 947
HindIII AAGCTT 2 cut(s) 563, 766
HinfI GANTC 6 cut(s) 13, 500, 856, 1026, 1137, 1472
HpaII CCGG 5 cut(s) 312, 431, 928, 1083, 1389
HphI GGTGA 3 cut(s) 449, 505, 1028
Hpy166II GTNNAC 9 cut(s) 205, 256, 278, 402, 428, 746, 947, 1038, 1099
Hpy188I TCNGA 4 cut(s) 12, 20, 1136, 1471
Hpy8I GTNNAC 9 cut(s) 205, 256, 278, 402, 428, 746, 947, 1038, 1099
Hpy99I CGWCG 2 cut(s) 1433, 1457
HpyAV CCTTC 8 cut(s) 76, 618, 682, 697, 1196, 1280, 1309, 1337
HpyCH4III ACNGT 1 cut(s) 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 782, 1125, 1241, 1401, 1425, 1449
HpyF3I CTNAG 6 cut(s) 17, 326, 539, 821, 1221, 1245
Hsp92II CATG 6 cut(s) 85, 100, 115, 784, 812, 1111
HspAI GCGC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 1360
Kzo9I GATC 5 cut(s) 270, 354, 595, 1360, 1380
LmnI GCTCC 2 cut(s) 185, 1249
Lsp1109I GCAGC 3 cut(s) 788, 921, 1023
LweI GCATC 5 cut(s) 15, 47, 523, 1258, 1357
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 1 cut(s) 1061
MaeIII GTNAC 4 cut(s) 114, 437, 728, 1225
MalI GATC 5 cut(s) 272, 356, 597, 1362, 1382
MbiI CCGCTC 2 cut(s) 138, 1128
MboI GATC 5 cut(s) 270, 354, 595, 1360, 1380
MboII GAAGA 8 cut(s) 362, 858, 861, 1270, 1331, 1433, 1457, 1467
MflI RGATCY 2 cut(s) 595, 1380
MhlI GDGCHC 4 cut(s) 404, 1101, 1220, 1254
MlsI TGGCCA 1 cut(s) 219
MluCI AATT 8 cut(s) 577, 787, 828, 845, 961, 1197, 1332, 1489
MluNI TGGCCA 1 cut(s) 219
MlyI GAGTC 1 cut(s) 494
Mox20I TGGCCA 1 cut(s) 219
MroXI GAANNNNTTC 1 cut(s) 1465
MscI TGGCCA 1 cut(s) 219
MseI TTAA 1 cut(s) 1208
MslI CAYNNNNRTG 3 cut(s) 168, 385, 1106
Msp20I TGGCCA 1 cut(s) 219
MspA1I CMGCKG 2 cut(s) 185, 1014
MspI CCGG 5 cut(s) 312, 431, 928, 1083, 1389
MspR9I CCNGG 4 cut(s) 312, 432, 750, 1083
Mva1269I GAATGC 3 cut(s) 1161, 1430, 1454
MvaI CCWGG 1 cut(s) 750
MwoI GCNNNNNNNGC 6 cut(s) 782, 1125, 1241, 1401, 1425, 1449
NciI CCSGG 3 cut(s) 312, 432, 1083
NcoI CCATGG 2 cut(s) 96, 780
NdeI CATATG 1 cut(s) 222
NdeII GATC 5 cut(s) 270, 354, 595, 1360, 1380
NlaIII CATG 6 cut(s) 85, 100, 115, 784, 812, 1111
NlaIV GGNNCC 1 cut(s) 636
NmeAIII GCCGAG 1 cut(s) 1137
NmuCI GTSAC 2 cut(s) 114, 437
NspI RCATGY 1 cut(s) 1111
OliI CACNNNNGTG 1 cut(s) 385
PciI ACATGT 1 cut(s) 1107
PcsI WCGNNNNNNNCGW 2 cut(s) 345, 366
PctI GAATGC 3 cut(s) 1161, 1430, 1454
PdmI GAANNNNTTC 1 cut(s) 1465
PfeI GAWTC 5 cut(s) 13, 856, 1026, 1137, 1472
PflMI CCANNNNNTGG 1 cut(s) 814
PkrI GCNGC 3 cut(s) 778, 911, 1013
PleI GAGTC 1 cut(s) 494
PpsI GAGTC 1 cut(s) 494
PpuMI RGGWCCY 1 cut(s) 556
PscI ACATGT 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 1254
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 748
PspEI GGTNACC 1 cut(s) 1225
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 1 cut(s) 636
PspPI GGNCC 7 cut(s) 103, 428, 556, 634, 743, 817, 976
PspPPI RGGWCCY 1 cut(s) 556
PstI CTGCAG 1 cut(s) 1237
PsuI RGATCY 2 cut(s) 595, 1380
RsaI GTAC 2 cut(s) 506, 754
RsaNI GTAC 2 cut(s) 505, 753
RseI CAYNNNNRTG 3 cut(s) 168, 385, 1106
SacI GAGCTC 1 cut(s) 1254
SalI GTCGAC 1 cut(s) 945
SaqAI TTAA 1 cut(s) 1208
SatI GCNGC 3 cut(s) 777, 910, 1012
Sau3AI GATC 5 cut(s) 270, 354, 595, 1360, 1380
Sau96I GGNCC 7 cut(s) 103, 428, 556, 634, 743, 817, 976
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 4 cut(s) 312, 432, 750, 1083
SduI GDGCHC 4 cut(s) 404, 1101, 1220, 1254
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 5 cut(s) 15, 47, 523, 1258, 1357
SfcI CTRYAG 1 cut(s) 1233
SinI GGWCC 6 cut(s) 103, 428, 556, 634, 743, 817
SmiMI CAYNNNNRTG 3 cut(s) 168, 385, 1106
SmlI CTYRAG 7 cut(s) 129, 260, 560, 644, 679, 794, 1350
SmoI CTYRAG 7 cut(s) 129, 260, 560, 644, 679, 794, 1350
Sse9I AATT 8 cut(s) 577, 787, 828, 845, 961, 1197, 1332, 1489
SsiI CCGC 6 cut(s) 54, 101, 136, 183, 1014, 1128
SspMI CTAG 1 cut(s) 1061
SstI GAGCTC 1 cut(s) 1254
StyD4I CCNGG 4 cut(s) 310, 430, 748, 1081
StyI CCWWGG 2 cut(s) 96, 780
TaaI ACNGT 1 cut(s) 842
TaqI TCGA 6 cut(s) 339, 360, 369, 660, 946, 1087
TasI AATT 8 cut(s) 577, 787, 828, 845, 961, 1197, 1332, 1489
TfiI GAWTC 5 cut(s) 13, 856, 1026, 1137, 1472
Tru1I TTAA 1 cut(s) 1208
Tru9I TTAA 1 cut(s) 1208
TscAI CASTG 6 cut(s) 76, 409, 630, 847, 1126, 1225
TseFI GTSAC 2 cut(s) 114, 437
TseI GCWGC 3 cut(s) 776, 909, 1011
Tsp45I GTSAC 2 cut(s) 114, 437
TspDTI ATGAA 3 cut(s) 255, 979, 1356
TspRI CASTG 6 cut(s) 76, 409, 630, 847, 1126, 1225
Van91I CCANNNNNTGG 1 cut(s) 814
VneI GTGCAC 2 cut(s) 400, 1097
VpaK11BI GGWCC 6 cut(s) 103, 428, 556, 634, 743, 817
XapI RAATTY 2 cut(s) 577, 828
XceI RCATGY 1 cut(s) 1111
XmiI GTMKAC 3 cut(s) 204, 255, 946
XmnI GAANNNNTTC 1 cut(s) 1465
XspI CTAG 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.