Rh2AG188600

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009595
Physical Location & Seq
Reverse (-)
18532213 .. 18543697
11485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG188600.1

Sequence Viewer

Length: 849 bp
ATGGACCCTCACTCTCACTCTCACCCGAAGCCGCGCGTGGTGGTATGCGGCGGCGGCGTAATCGGCGTCTGCACCGCTTACTTCCTCGCCAAAAACGGCGCCGTCGTCACTCTCGTCGAGAAATCCTCCGTCGCCTGCGCCGCCTCAGGAAAAGCCGGCGGATTCCTCGCCCTCGATTGGTGCGACGGCGGGCCCCTCGAGTCCCTCGCCCGCGCCAGCTTCAATCTCCACCGCTCGCTCGCTCACGAGCTCGGCGGCCCCGCATCCTACGGCTACCGGCCGCTCAACACTCTCAGCCTCACCGTAACGGAATCCCAGACCTCCAAGCCTCACTCCGGCAAGTCCGATTTGCCGGACTGGGTCGACGGGCCGGGTCGGAGCCCGAGAACGATCGGGACCACCGCGACCACGGCCCAGGTCCACCCCCAGCTCTTCTGCCGAACCCTAATTTCCAAAGCGATTGAGGATTACGGCGTGCAAGTGGTGATTGGGAAATTGGAGGAGGTTGGAGTCGTTGAAGGGCGAGTTGACTCGGTGGTGTTGGAAGGAGGGCGAGCTATAAACTCAGACGCCGTCGTTTTGGCGCTTGGGCCGTGGTGTGGGAAATTTGAGCTGTTGTCGTCTTTGTTCAGAGTGTATGGTTTGAAAGCTCATAGCATTGTGTTAAGGCCTAAAGAACCTGAGGCCATAACACCTCATGCTCTTTTCTTGAGTTATTACCCAGCTCAAGGAGGAAAGCCATTGGACCCAGAAGTGTATCCACGTCCCACAGCCCGATCTGAACCAAATAGCAATAAGAGGCGGCCGCCTCCTTCTCCTCCGCTGAGACGCTCAGACCCCCGCCGCTGA

Protein Analysis

282

Amino Acids

30.11

Weight (kDa)

9.14

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 12 - 232 5.3e-28 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccBSI CCGCTC 2 cut(s) 234, 283
AccI GTMKAC 1 cut(s) 363
AccII CGCG 4 cut(s) 34, 36, 213, 404
AcoI YGGCCR 2 cut(s) 278, 803
AcsI RAATTY 1 cut(s) 605
AcyI GRCGYC 3 cut(s) 66, 99, 570
AfiI CCNNNNNNNGG 4 cut(s) 177, 335, 599, 728
AgsI TTSAA 3 cut(s) 223, 518, 646
AjiI CACGTC 1 cut(s) 764
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 7 cut(s) 219, 250, 430, 557, 613, 650, 725
AluI AGCT 7 cut(s) 219, 250, 430, 557, 613, 650, 725
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 820
Ama87I CYCGRG 2 cut(s) 197, 382
AoxI GGCC 9 cut(s) 191, 256, 278, 368, 411, 590, 668, 684, 803
ApaI GGGCCC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 605
AspLEI GCGC 5 cut(s) 36, 101, 140, 215, 586
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 3 cut(s) 14, 292, 496
AvaI CYCGRG 2 cut(s) 197, 382
AvaII GGWCC 4 cut(s) 4, 396, 418, 745
AxyI CCTNAGG 2 cut(s) 145, 681
BaeGI GKGCMC 1 cut(s) 195
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 3 cut(s) 195, 252, 383
BauI CACGAG 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 252
BceAI ACGGC 8 cut(s) 86, 112, 202, 286, 426, 487, 557, 577
BciT130I CCWGG 1 cut(s) 416
BciVI GTATCC 1 cut(s) 768
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 820
BfoI RGCGCY 2 cut(s) 102, 587
BfuI GTATCC 1 cut(s) 768
Bme1390I CCNGG 2 cut(s) 372, 416
Bme18I GGWCC 4 cut(s) 4, 396, 418, 745
BmeT110I CYCGRG 2 cut(s) 197, 382
BmgBI CACGTC 1 cut(s) 764
BmiI GGNNCC 8 cut(s) 6, 100, 193, 194, 259, 380, 397, 747
BmrFI CCNGG 2 cut(s) 372, 416
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 1 cut(s) 272
BmuI ACTGGG 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpuEI CTTGAG 2 cut(s) 711, 730
BpuMI CCSGG 1 cut(s) 372
BsaHI GRCGYC 3 cut(s) 66, 99, 570
BsaJI CCNNGG 3 cut(s) 408, 414, 593
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 335, 599, 728
Bse118I RCCGGY 2 cut(s) 155, 276
Bse1I ACTGG 1 cut(s) 362
Bse21I CCTNAGG 2 cut(s) 145, 681
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 3 cut(s) 408, 414, 593
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 4 cut(s) 177, 335, 599, 728
BseMII CTCAG 6 cut(s) 159, 307, 579, 672, 815, 846
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 2 cut(s) 515, 807
BseSI GKGCMC 1 cut(s) 195
BseX3I CGGCCG 2 cut(s) 278, 803
BseYI CCCAGC 2 cut(s) 426, 721
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 4 cut(s) 34, 36, 213, 404
Bsh1285I CGRYCG 3 cut(s) 281, 393, 806
BshFI GGCC 9 cut(s) 193, 258, 280, 370, 413, 592, 670, 686, 805
BshNI GGYRCC 1 cut(s) 98
BsiEI CGRYCG 3 cut(s) 281, 393, 806
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 2 cut(s) 197, 382
BsiSI CCGG 5 cut(s) 156, 277, 336, 353, 371
BslFI GGGAC 3 cut(s) 187, 409, 750
BslI CCNNNNNNNGG 4 cut(s) 177, 335, 599, 728
BsmAI GTCTC 1 cut(s) 820
BsmBI CGTCTC 1 cut(s) 820
BsmFI GGGAC 3 cut(s) 187, 409, 750
BsnI GGCC 9 cut(s) 193, 258, 280, 370, 413, 592, 670, 686, 805
BsoBI CYCGRG 2 cut(s) 197, 382
Bsp120I GGGCCC 1 cut(s) 191
Bsp1286I GDGCHC 3 cut(s) 195, 252, 383
Bsp143I GATC 2 cut(s) 390, 776
BspANI GGCC 9 cut(s) 193, 258, 280, 370, 413, 592, 670, 686, 805
BspCNI CTCAG 6 cut(s) 158, 306, 578, 673, 816, 845
BspFNI CGCG 4 cut(s) 34, 36, 213, 404
BspLI GGNNCC 8 cut(s) 6, 100, 193, 194, 259, 380, 397, 747
BspQI GCTCTTC 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 98
BsrBI CCGCTC 2 cut(s) 234, 283
BsrFI RCCGGY 2 cut(s) 155, 276
BsrI ACTGG 1 cut(s) 362
BssAI RCCGGY 2 cut(s) 155, 276
BssECI CCNNGG 3 cut(s) 408, 414, 593
BssMI GATC 2 cut(s) 390, 776
BssNI GRCGYC 3 cut(s) 66, 99, 570
BssSI CACGAG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 3 cut(s) 66, 99, 570
BstC8I GCNNGC 9 cut(s) 136, 157, 191, 211, 217, 236, 240, 476, 555
BstDEI CTNAG 6 cut(s) 145, 293, 565, 681, 824, 832
BstDSI CCRYGG 2 cut(s) 408, 593
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 4 cut(s) 34, 36, 213, 404
BstH2I RGCGCY 2 cut(s) 102, 587
BstHHI GCGC 5 cut(s) 36, 101, 140, 215, 586
BstKTI GATC 2 cut(s) 393, 779
BstMAI GTCTC 1 cut(s) 820
BstMBI GATC 2 cut(s) 390, 776
BstMCI CGRYCG 3 cut(s) 281, 393, 806
BstMWI GCNNNNNNNGC 4 cut(s) 54, 63, 140, 410
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 370, 414
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 4 cut(s) 34, 36, 213, 404
BstZI CGGCCG 2 cut(s) 278, 803
Bsu36I CCTNAGG 2 cut(s) 145, 681
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 9 cut(s) 193, 258, 280, 370, 413, 592, 670, 686, 805
BtgI CCRYGG 2 cut(s) 408, 593
BtrI CACGTC 1 cut(s) 764
BtsCI GGATG 1 cut(s) 263
Cac8I GCNNGC 9 cut(s) 136, 157, 191, 211, 217, 236, 240, 476, 555
CciNI GCGGCCGC 1 cut(s) 803
CfoI GCGC 5 cut(s) 36, 101, 140, 215, 586
Cfr10I RCCGGY 2 cut(s) 155, 276
CseI GACGC 3 cut(s) 55, 578, 837
CviAII CATG 1 cut(s) 698
DdeI CTNAG 6 cut(s) 145, 293, 565, 681, 824, 832
DinI GGCGCC 1 cut(s) 100
DpnI GATC 2 cut(s) 392, 778
DpnII GATC 2 cut(s) 390, 776
EaeI YGGCCR 2 cut(s) 278, 803
EagI CGGCCG 2 cut(s) 278, 803
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
EciI GGCGGA 1 cut(s) 174
Ecl136II GAGCTC 1 cut(s) 250
EclXI CGGCCG 2 cut(s) 278, 803
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 3 cut(s) 195, 252, 383
Eco47I GGWCC 4 cut(s) 4, 396, 418, 745
Eco52I CGGCCG 2 cut(s) 278, 803
Eco53kI GAGCTC 1 cut(s) 250
Eco81I CCTNAGG 2 cut(s) 145, 681
Eco88I CYCGRG 2 cut(s) 197, 382
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 414
EcoT38I GRGCYC 3 cut(s) 195, 252, 383
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 820
FaeI CATG 1 cut(s) 701
FaiI YATR 6 cut(s) 46, 560, 639, 654, 689, 699
FaqI GGGAC 3 cut(s) 187, 409, 750
FatI CATG 1 cut(s) 697
FauI CCCGC 3 cut(s) 182, 218, 268
FblI GTMKAC 1 cut(s) 363
FokI GGATG 1 cut(s) 250
FriOI GRGCYC 3 cut(s) 195, 252, 383
GlaI GCGC 5 cut(s) 35, 100, 139, 214, 585
GsaI CCCAGC 2 cut(s) 430, 725
HaeII RGCGCY 2 cut(s) 102, 587
HaeIII GGCC 9 cut(s) 193, 258, 280, 370, 413, 592, 670, 686, 805
HapII CCGG 5 cut(s) 156, 277, 336, 353, 371
HgaI GACGC 3 cut(s) 55, 578, 837
HhaI GCGC 5 cut(s) 36, 101, 140, 215, 586
Hin1I GRCGYC 3 cut(s) 66, 99, 570
Hin1II CATG 1 cut(s) 701
Hin6I GCGC 5 cut(s) 34, 99, 138, 213, 584
HinP1I GCGC 5 cut(s) 34, 99, 138, 213, 584
HincII GTYRAC 2 cut(s) 364, 529
HindII GTYRAC 2 cut(s) 364, 529
HinfI GANTC 5 cut(s) 162, 200, 311, 510, 530
HpaII CCGG 5 cut(s) 156, 277, 336, 353, 371
HphI GGTGA 3 cut(s) 14, 292, 496
Hpy166II GTNNAC 3 cut(s) 364, 421, 529
Hpy188I TCNGA 6 cut(s) 346, 378, 568, 632, 781, 835
Hpy188III TCNNGA 5 cut(s) 118, 147, 245, 394, 709
Hpy8I GTNNAC 3 cut(s) 364, 421, 529
Hpy99I CGWCG 6 cut(s) 107, 119, 134, 188, 368, 578
HpyAV CCTTC 3 cut(s) 512, 539, 822
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 763
HpyCH4V TGCA 2 cut(s) 72, 478
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 63, 140, 410
HpyF3I CTNAG 6 cut(s) 145, 293, 565, 681, 824, 832
HpySE526I ACGT 1 cut(s) 763
Hsp92I GRCGYC 3 cut(s) 66, 99, 570
Hsp92II CATG 1 cut(s) 701
HspAI GCGC 5 cut(s) 34, 99, 138, 213, 584
KasI GGCGCC 1 cut(s) 98
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 2 cut(s) 390, 776
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 1 cut(s) 378
LweI GCATC 1 cut(s) 272
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 2 cut(s) 106, 304
MalI GATC 2 cut(s) 392, 778
MbiI CCGCTC 2 cut(s) 234, 283
MboI GATC 2 cut(s) 390, 776
MboII GAAGA 1 cut(s) 424
MhlI GDGCHC 3 cut(s) 195, 252, 383
MluCI AATT 3 cut(s) 447, 494, 605
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 3 cut(s) 209, 519, 524
MmeI TCCRAC 3 cut(s) 356, 487, 522
MroNI GCCGGC 1 cut(s) 155
MseI TTAA 1 cut(s) 665
MspA1I CMGCKG 2 cut(s) 823, 846
MspI CCGG 5 cut(s) 156, 277, 336, 353, 371
MspR9I CCNGG 2 cut(s) 372, 416
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 4 cut(s) 34, 36, 213, 404
MwoI GCNNNNNNNGC 4 cut(s) 54, 63, 140, 410
NaeI GCCGGC 1 cut(s) 157
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 1 cut(s) 372
NdeII GATC 2 cut(s) 390, 776
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 1 cut(s) 701
NlaIV GGNNCC 8 cut(s) 6, 100, 193, 194, 259, 380, 397, 747
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 106
NotI GCGGCCGC 1 cut(s) 803
PaeR7I CTCGAG 1 cut(s) 197
PceI AGGCCT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 437
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 111, 180
PdiI GCCGGC 1 cut(s) 157
PfeI GAWTC 2 cut(s) 162, 311
PflFI GACNNNGTC 2 cut(s) 359, 572
Ple19I CGATCG 1 cut(s) 393
PleI GAGTC 3 cut(s) 208, 518, 524
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 3 cut(s) 208, 518, 524
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 414
PspFI CCCAGC 2 cut(s) 426, 721
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 8 cut(s) 6, 100, 193, 194, 259, 380, 397, 747
PspOMI GGGCCC 1 cut(s) 191
PspXI VCTCGAGB 1 cut(s) 197
PsyI GACNNNGTC 2 cut(s) 359, 572
PvuI CGATCG 1 cut(s) 393
SacI GAGCTC 1 cut(s) 252
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 1 cut(s) 665
Sau3AI GATC 2 cut(s) 390, 776
SchI GAGTC 3 cut(s) 209, 519, 524
ScrFI CCNGG 2 cut(s) 372, 416
SduI GDGCHC 3 cut(s) 195, 252, 383
SfaNI GCATC 1 cut(s) 272
SfoI GGCGCC 1 cut(s) 100
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 4 cut(s) 4, 396, 418, 745
SlaI CTCGAG 1 cut(s) 197
SmlI CTYRAG 3 cut(s) 197, 709, 726
SmoI CTYRAG 3 cut(s) 197, 709, 726
Sse9I AATT 3 cut(s) 447, 494, 605
SseBI AGGCCT 1 cut(s) 670
SspDI GGCGCC 1 cut(s) 98
SstI GAGCTC 1 cut(s) 252
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 2 cut(s) 370, 414
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 766
TaqI TCGA 4 cut(s) 117, 174, 198, 363
TasI AATT 3 cut(s) 447, 494, 605
TfiI GAWTC 2 cut(s) 162, 311
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspGWI ACGGA 2 cut(s) 118, 323
Tth111I GACNNNGTC 2 cut(s) 359, 572
VpaK11BI GGWCC 4 cut(s) 4, 396, 418, 745
XapI RAATTY 1 cut(s) 605
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.