Rorug02G0261900

Rare lipoprotein A (RlpA)-like double-psi beta-barrel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009571
Physical Location & Seq
Forward (+)
27483464 .. 27486030
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0261900.1

Sequence Viewer

Length: 1620 bp
ATGGCTCTGGAAGCTCCCCTTCTTCACAAACCCACAACCCGATTTTCATTGAACCACCGCCGTGTCGCCGCCTCTGCCGCCGCTTTTCTTCGACTTCACAGAGGACCTCATTCCCCATTCTCCGTCGGGAAAACCACTCGTCGTATCCCAACTACCATATTGGCGGCTAAGGCGGGTGCTGTGACGGAGCCGGTGATTCAATTGGAGTCAAAGCCGGTGAGAATAGTAGCCCTCGTCGGCAAAGGTAGCGTCAGCCCTCTCAAGGCTACACCTTGGGAAGACGTCATGCTCCACACCGTGAAACGACTGAAATGGGTTGACGAAGGGTATGAGATGGTTGTATTTACGGATGATATCTGCCAGTCTAATGATCAAAATGTGGTGAGTTTCCGGATGGAATTACAACGTGCAGATATTTTGGTAATTGTTGCTGTTACGAAGCAAGAATCCGTCGAGTGGATTCAAGCTAATGGTGGGAATATTCAAAACATAATCTGTTTTGACTCATCTCCCAATTTGCAAAACAAGTTAGGAGGATCTTATATTCATAGTGAAGCCAAAGGAGATATCTTTGGCAAAATGTTTGACACTGCCGAATCAAAGAAAAGCAGTGAATCAATGGAAGTGGTACAGACTGTGTCACAGGCGTGGGAGCGGCATAATTCTGATGATATAAGGTTTTGTTTGTTGGTTATAATCAATGGGTATATAAGGCCGGTGCCAATCCTGCAGAATCTGAGATCGAAAGGTCTTTCTACTCTGAGCTGCATGGTGAAGAACTGTGGACCTCAGATACTGAACTGCCTCCTGGATCCCAATTGTAGGAAAGCTCTTCAATGCTTGAATGAGTGCAGTCCGGTGGATCAAGTGTGTAACTATCGGTGCATTGCTTCGTATGAGAGTCCAGTTCTAGAAGCGTTTTCTCTCTGTGTGTTGCAGAAGCACAATTGTCTTGGGTTGGATGCGAAGATCCCTGACAAACCATATGTGCCACCCATGGTTAAATTTCGAGGAAAGGACTTGTGCCACGAAACTGCTGAAGATCTTTTTGTTGGTTGGTTGGGAAGTTTGGATTGGAGTTGGCGTGTCGTGGCAGGGCAGAACCCAGCATATGATCAATTCCCTTGCCAGTACCAATTATTTTACAGGGGCAAGGCAAAAGGTTCATTTTGGTATGAGCCGGTTTTTCAAGTGAAAACGCTGGAGGGGAAGATGGTTTGGAGGAGGCGGAAGTATCGTGTCAAGAGAAACAAAGTCCCAGGGACATTCCAATTCAGTGTCTTGGACAATGGGGTTGTTTCAAATGAGTCTTGGACAATCGTGGATGTAGCTGATGATCTTAGTTGGGGTTTGTTTTACTACTCTGGCGCTGCAAGAGTTGCTGGACAATCCTATACTGGTGCTGTGCTTGTCAGTCCAGATGGGGCATACCCAAATGATGTGCATAGGAGCAGATTAGTTTCCGCTTTGGAGAACTGTGGAATTAAAGAGTGGGAGCTGTTTACAGTTAACAATTCTTCGTGTGCTAATCCTCCATTGGGGATTCCTGAGGGTTCACGTTTGCATTCCGTGATAGAAGTTAAAGATCAGAAATCTTTGTCGGATTTGGCAGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006949 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0016020 GO:0030054 GO:0030312 GO:0032502 GO:0044464 GO:0048646 GO:0048856 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

539

Amino Acids

60.18

Weight (kDa)

8.45

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VDE PF07137 255 - 505 3.3e-27 VDE lipocalin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AatII GACGTC 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 718
AccBSI CCGCTC 1 cut(s) 655
AccIII TCCGGA 1 cut(s) 390
AciI CCGC 9 cut(s) 58, 69, 78, 81, 164, 173, 655, 1228, 1464
AclWI GGATC 5 cut(s) 544, 806, 819, 870, 964
AcsI RAATTY 1 cut(s) 1004
AcuI CTGAAG 1 cut(s) 1059
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 298
AfaI GTAC 2 cut(s) 630, 1133
AfiI CCNNNNNNNGG 4 cut(s) 262, 456, 822, 1538
AgsI TTSAA 8 cut(s) 52, 200, 464, 485, 836, 844, 1190, 1302
AjnI CCWGG 2 cut(s) 807, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 1057, 1089
AleI CACNNNNGTG 2 cut(s) 60, 646
AluBI AGCT 6 cut(s) 14, 467, 765, 830, 1331, 1498
AluI AGCT 6 cut(s) 14, 467, 765, 830, 1331, 1498
AlwI GGATC 5 cut(s) 544, 806, 819, 870, 964
AlwNI CAGNNNCTG 2 cut(s) 736, 796
Aor13HI TCCGGA 1 cut(s) 390
AoxI GGCC 1 cut(s) 713
ApeKI GCWGC 2 cut(s) 765, 1370
ApoI RAATTY 1 cut(s) 1004
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
AspLEI GCGC 1 cut(s) 1370
AspS9I GGNCC 2 cut(s) 104, 785
AsuHPI GGTGA 4 cut(s) 205, 229, 394, 784
AvaII GGWCC 2 cut(s) 104, 785
AxyI CCTNAGG 1 cut(s) 1548
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 718
BbsI GAAGAC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 752, 1357
BccI CCATC 4 cut(s) 328, 388, 1207, 1415
BceAI ACGGC 1 cut(s) 45
BciT130I CCWGG 2 cut(s) 809, 1260
BciVI GTATCC 1 cut(s) 155
BclI TGATCA 2 cut(s) 370, 1114
BfaI CTAG 1 cut(s) 911
BfmI CTRYAG 1 cut(s) 728
BfoI RGCGCY 1 cut(s) 1371
BfuI GTATCC 1 cut(s) 155
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 7 cut(s) 69, 78, 81, 165, 656, 766, 1371
BlsI GCNGC 7 cut(s) 70, 79, 82, 166, 657, 767, 1372
Bme1390I CCNGG 2 cut(s) 809, 1260
Bme18I GGWCC 2 cut(s) 104, 785
BmgT120I GGNCC 2 cut(s) 104, 785
BmiI GGNNCC 3 cut(s) 189, 720, 813
BmrFI CCNGG 2 cut(s) 809, 1260
BmsI GCATC 1 cut(s) 952
BpiI GAAGAC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 1223
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 4 cut(s) 272, 996, 1258, 1259
BsaWI WCCGGW 2 cut(s) 390, 856
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 456, 822, 1538
Bse118I RCCGGY 4 cut(s) 190, 214, 715, 1180
Bse1I ACTGG 4 cut(s) 361, 905, 1129, 1402
Bse21I CCTNAGG 1 cut(s) 1548
Bse3DI GCAATG 1 cut(s) 885
BseAI TCCGGA 1 cut(s) 390
BseBI CCWGG 2 cut(s) 809, 1260
BseDI CCNNGG 4 cut(s) 272, 996, 1258, 1259
BseGI GGATG 4 cut(s) 355, 399, 967, 1330
BseLI CCNNNNNNNGG 4 cut(s) 262, 456, 822, 1538
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 4 cut(s) 728, 752, 803, 1539
BseNI ACTGG 4 cut(s) 361, 905, 1129, 1402
BseRI GAGGAG 1 cut(s) 1237
BseXI GCAGC 2 cut(s) 752, 1357
BseYI CCCAGC 1 cut(s) 1105
BsgI GTGCAG 2 cut(s) 429, 871
BshFI GGCC 1 cut(s) 715
BshNI GGYRCC 1 cut(s) 718
BsiSI CCGG 6 cut(s) 191, 215, 391, 716, 857, 1181
BslFI GGGAC 2 cut(s) 1241, 1276
BslI CCNNNNNNNGG 4 cut(s) 262, 456, 822, 1538
BsmFI GGGAC 2 cut(s) 1241, 1276
BsmI GAATGC 1 cut(s) 1564
BsnI GGCC 1 cut(s) 715
Bsp13I TCCGGA 1 cut(s) 390
Bsp19I CCATGG 1 cut(s) 996
BspACI CCGC 9 cut(s) 58, 69, 78, 81, 164, 173, 655, 1228, 1464
BspANI GGCC 1 cut(s) 715
BspCNI CTCAG 4 cut(s) 729, 753, 802, 1540
BspEI TCCGGA 1 cut(s) 390
BspLI GGNNCC 3 cut(s) 189, 720, 813
BspMAI CTGCAG 1 cut(s) 732
BspPI GGATC 5 cut(s) 544, 806, 819, 870, 964
BspQI GCTCTTC 1 cut(s) 837
BspT107I GGYRCC 1 cut(s) 718
BsrBI CCGCTC 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 885
BsrFI RCCGGY 4 cut(s) 190, 214, 715, 1180
BsrI ACTGG 4 cut(s) 361, 905, 1129, 1402
BssAI RCCGGY 4 cut(s) 190, 214, 715, 1180
BssECI CCNNGG 4 cut(s) 272, 996, 1258, 1259
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 2 cut(s) 272, 996
Bst2UI CCWGG 2 cut(s) 809, 1260
Bst4CI ACNGT 5 cut(s) 298, 637, 782, 1478, 1507
Bst6I CTCTTC 1 cut(s) 837
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 1379
BstDEI CTNAG 6 cut(s) 168, 737, 761, 789, 1340, 1548
BstDSI CCRYGG 1 cut(s) 996
BstF5I GGATG 4 cut(s) 355, 399, 967, 1330
BstH2I RGCGCY 1 cut(s) 1371
BstHHI GCGC 1 cut(s) 1370
BstMWI GCNNNNNNNGC 7 cut(s) 11, 74, 77, 170, 246, 727, 1379
BstNI CCWGG 2 cut(s) 809, 1260
BstSCI CCNGG 2 cut(s) 807, 1258
BstSFI CTRYAG 1 cut(s) 728
BstV1I GCAGC 2 cut(s) 752, 1357
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 4 cut(s) 536, 811, 969, 1042
BstYI RGATCY 4 cut(s) 536, 811, 969, 1042
Bsu36I CCTNAGG 1 cut(s) 1548
BsuI GTATCC 1 cut(s) 155
BsuRI GGCC 1 cut(s) 715
BtgI CCRYGG 1 cut(s) 996
BtsCI GGATG 4 cut(s) 355, 399, 967, 1330
BtsI GCAGTG 2 cut(s) 588, 616
BtsIMutI CAGTG 3 cut(s) 588, 616, 1282
CaiI CAGNNNCTG 2 cut(s) 736, 796
CfoI GCGC 1 cut(s) 1370
Cfr10I RCCGGY 4 cut(s) 190, 214, 715, 1180
Cfr13I GGNCC 2 cut(s) 104, 785
CseI GACGC 1 cut(s) 238
Csp6I GTAC 2 cut(s) 629, 1132
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 3 cut(s) 286, 769, 997
CviQI GTAC 2 cut(s) 629, 1132
DdeI CTNAG 6 cut(s) 168, 737, 761, 789, 1340, 1548
DraIII CACNNNGTG 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 837
EarI CTCTTC 1 cut(s) 837
EciI GGCGGA 1 cut(s) 1243
Eco130I CCWWGG 2 cut(s) 272, 996
Eco32I GATATC 2 cut(s) 355, 568
Eco47I GGWCC 2 cut(s) 104, 785
Eco57I CTGAAG 1 cut(s) 1059
Eco81I CCTNAGG 1 cut(s) 1548
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 807, 1258
EcoRV GATATC 2 cut(s) 355, 568
EcoT14I CCWWGG 2 cut(s) 272, 996
ErhI CCWWGG 2 cut(s) 272, 996
FaeI CATG 3 cut(s) 289, 772, 1000
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 2 cut(s) 1241, 1276
FatI CATG 3 cut(s) 285, 768, 996
FauI CCCGC 1 cut(s) 166
FauNDI CATATG 2 cut(s) 985, 1111
FbaI TGATCA 2 cut(s) 370, 1114
Fnu4HI GCNGC 7 cut(s) 69, 78, 81, 165, 656, 766, 1371
FokI GGATG 4 cut(s) 362, 406, 974, 1337
Fsp4HI GCNGC 7 cut(s) 69, 78, 81, 165, 656, 766, 1371
FspBI CTAG 1 cut(s) 911
GlaI GCGC 1 cut(s) 1369
GluI GCNGC 7 cut(s) 69, 78, 81, 165, 656, 766, 1371
GsaI CCCAGC 1 cut(s) 1109
GsuI CTGGAG 1 cut(s) 1223
HaeII RGCGCY 1 cut(s) 1371
HaeIII GGCC 1 cut(s) 715
HapII CCGG 6 cut(s) 191, 215, 391, 716, 857, 1181
HgaI GACGC 1 cut(s) 238
HhaI GCGC 1 cut(s) 1370
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 3 cut(s) 289, 772, 1000
Hin6I GCGC 1 cut(s) 1368
HinP1I GCGC 1 cut(s) 1368
HincII GTYRAC 2 cut(s) 319, 1510
HindII GTYRAC 2 cut(s) 319, 1510
HpaI GTTAAC 1 cut(s) 1510
HpaII CCGG 6 cut(s) 191, 215, 391, 716, 857, 1181
HphI GGTGA 4 cut(s) 205, 229, 394, 784
Hpy166II GTNNAC 5 cut(s) 319, 785, 1503, 1510, 1556
Hpy188I TCNGA 6 cut(s) 667, 738, 762, 792, 1590, 1603
Hpy188III TCNNGA 7 cut(s) 8, 127, 391, 911, 1243, 1418, 1547
Hpy8I GTNNAC 5 cut(s) 319, 785, 1503, 1510, 1556
Hpy99I CGWCG 4 cut(s) 128, 144, 239, 455
HpyAV CCTTC 2 cut(s) 29, 317
HpyCH4III ACNGT 5 cut(s) 298, 637, 782, 1478, 1507
HpyCH4IV ACGT 3 cut(s) 282, 406, 1558
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 74, 77, 170, 246, 727, 1379
HpyF3I CTNAG 6 cut(s) 168, 737, 761, 789, 1340, 1548
HpySE526I ACGT 3 cut(s) 282, 406, 1558
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 3 cut(s) 289, 772, 1000
HspAI GCGC 1 cut(s) 1368
Kpn2I TCCGGA 1 cut(s) 390
Ksp22I TGATCA 2 cut(s) 370, 1114
KspAI GTTAAC 1 cut(s) 1510
LguI GCTCTTC 1 cut(s) 837
LmnI GCTCC 6 cut(s) 19, 187, 294, 652, 1449, 1495
Lsp1109I GCAGC 2 cut(s) 752, 1357
LweI GCATC 1 cut(s) 952
MaeI CTAG 1 cut(s) 911
MaeII ACGT 3 cut(s) 282, 406, 1558
MaeIII GTNAC 4 cut(s) 181, 433, 639, 872
MbiI CCGCTC 1 cut(s) 655
MboII GAAGA 9 cut(s) 14, 80, 290, 787, 824, 979, 1052, 1222, 1509
MfeI CAATTG 3 cut(s) 200, 817, 946
MflI RGATCY 4 cut(s) 536, 811, 969, 1042
MlyI GAGTC 4 cut(s) 215, 497, 910, 1316
MmeI TCCRAC 2 cut(s) 939, 1581
MroI TCCGGA 1 cut(s) 390
MseI TTAA 4 cut(s) 1002, 1485, 1509, 1581
MslI CAYNNNNRTG 2 cut(s) 60, 646
MspI CCGG 6 cut(s) 191, 215, 391, 716, 857, 1181
MspR9I CCNGG 2 cut(s) 809, 1260
MunI CAATTG 3 cut(s) 200, 817, 946
Mva1269I GAATGC 1 cut(s) 1564
MvaI CCWGG 2 cut(s) 809, 1260
MwoI GCNNNNNNNGC 7 cut(s) 11, 74, 77, 170, 246, 727, 1379
NcoI CCATGG 1 cut(s) 996
NdeI CATATG 2 cut(s) 985, 1111
NlaIII CATG 3 cut(s) 289, 772, 1000
NlaIV GGNNCC 3 cut(s) 189, 720, 813
NmuCI GTSAC 2 cut(s) 181, 639
OliI CACNNNNGTG 2 cut(s) 60, 646
PasI CCCWGGG 1 cut(s) 1259
PciSI GCTCTTC 1 cut(s) 837
PctI GAATGC 1 cut(s) 1564
PfeI GAWTC 7 cut(s) 196, 446, 460, 596, 614, 733, 1543
PflFI GACNNNGTC 1 cut(s) 637
PfoI TCCNGGA 1 cut(s) 807
PkrI GCNGC 7 cut(s) 70, 79, 82, 166, 657, 767, 1372
PleI GAGTC 4 cut(s) 214, 497, 909, 1315
PpsI GAGTC 4 cut(s) 214, 497, 909, 1315
PpuMI RGGWCCY 1 cut(s) 104
PsiI TTATAA 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 807, 1258
PspFI CCCAGC 1 cut(s) 1105
PspGI CCWGG 2 cut(s) 807, 1258
PspN4I GGNNCC 3 cut(s) 189, 720, 813
PspPI GGNCC 2 cut(s) 104, 785
PspPPI RGGWCCY 1 cut(s) 104
PstI CTGCAG 1 cut(s) 732
PstNI CAGNNNCTG 2 cut(s) 736, 796
PsuI RGATCY 4 cut(s) 536, 811, 969, 1042
PsyI GACNNNGTC 1 cut(s) 637
RsaI GTAC 2 cut(s) 630, 1133
RsaNI GTAC 2 cut(s) 629, 1132
RseI CAYNNNNRTG 2 cut(s) 60, 646
SapI GCTCTTC 1 cut(s) 837
SaqAI TTAA 4 cut(s) 1002, 1485, 1509, 1581
SatI GCNGC 7 cut(s) 69, 78, 81, 165, 656, 766, 1371
Sau96I GGNCC 2 cut(s) 104, 785
SchI GAGTC 4 cut(s) 215, 497, 910, 1316
ScrFI CCNGG 2 cut(s) 809, 1260
SfaNI GCATC 1 cut(s) 952
SfcI CTRYAG 1 cut(s) 728
SinI GGWCC 2 cut(s) 104, 785
SmiMI CAYNNNNRTG 2 cut(s) 60, 646
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SsiI CCGC 9 cut(s) 58, 69, 78, 81, 164, 173, 655, 1228, 1464
SspI AATATT 1 cut(s) 481
SspMI CTAG 1 cut(s) 911
StyD4I CCNGG 2 cut(s) 807, 1258
StyI CCWWGG 2 cut(s) 272, 996
TaaI ACNGT 5 cut(s) 298, 637, 782, 1478, 1507
TaiI ACGT 3 cut(s) 285, 409, 1561
TaqI TCGA 4 cut(s) 91, 453, 743, 1009
TauI GCSGC 5 cut(s) 71, 80, 83, 167, 658
TfiI GAWTC 7 cut(s) 196, 446, 460, 596, 614, 733, 1543
Tru1I TTAA 4 cut(s) 1002, 1485, 1509, 1581
Tru9I TTAA 4 cut(s) 1002, 1485, 1509, 1581
TscAI CASTG 3 cut(s) 595, 616, 1282
TseFI GTSAC 2 cut(s) 181, 639
TseI GCWGC 2 cut(s) 765, 1370
Tsp45I GTSAC 2 cut(s) 181, 639
TspDTI ATGAA 3 cut(s) 36, 536, 1155
TspGWI ACGGA 5 cut(s) 112, 200, 362, 439, 1558
TspRI CASTG 3 cut(s) 595, 616, 1282
Tth111I GACNNNGTC 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 104, 785
XapI RAATTY 1 cut(s) 1004
XbaI TCTAGA 1 cut(s) 910
XspI CTAG 1 cut(s) 911
ZraI GACGTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.