Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0007942

Physical Location & Seq

Genomic Coordinates Reverse (-)

34062431 .. 34064977

Gene Structure 2547 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0304000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGGCCGCAATGCTTCAGCTGAGTCCTCTGGTCTCAGCCACCCCAAGTTTCATAGCTCCCACTAACCAAAACGAACCCAAAGCCATAGCCAAGGACACTTCCCCAACTGGGTCACTAAGAAACTGTAAAACCATAAACCAAGTGAAGCAACTTCACTGTCACATCACTAAGACAGGCCTCAGTCACACACCCTCTAATGTCACCAAGCTAATTAGCGCATGCGCCGAAATGGGCACCTTCGAAAGCTTAGATTATGCCCGGAAAGCCTTTAACTTGTTTCTTGAAGAAGAAGAAGAAGAAACAAAGGGTGTATTGTTCATGTATAATTCTCTCATAAGGGGTTACTCTAGTGCTGGGCTTTGTGAGGAGGCTATTGGGCTCTATGTTCAGATGGTGCTTCAGGGTGTTTTGCCGGATAAGTTCACATTTCCGTTTGCACTGAGCGCGTGCTCAAAGATTGTGGCTTTCTGTGAAGGGGTTCAGCTGCACGGGTCGCTTGTGAAGATGGGTTTGGAGGAAGATGTGTTTATTGGGAATTCTTTGATTCATTTCTATGCAGAATGTGGGCACTTGGATTATGCCCAGAAGGTGTTTGATGAAATGCTTGAGAGAAACATCGTGTCGTGGACTAGTTTGATTTGTGGTTATGGTAGGAGGAATATGCCAAAGCAGGCTGTTTCTTTGTTTTTCGAGATGGTGGCTGCCGGTGTTAAGCCCAATTCGGTGACTATGGTGTGTGTCATTTCTGCTTGTGCAAAGTTGAAGGATGTTGCGTTGAGTGAGAGAGTGTGCGCTTACATTGGGGAGTCTGGACTCAAGACTAATATGCTTATGGTGAATTCACTTGTTGATATGTATATGAAATGTGGGGCTACTGATGCCGCAAAGCGGCTTTTTGATGAATGTGTGGATAAAAACTTGGTTCTTTACAACACGATTTTGTCAAATTATGTGCGCCAGGGACTGGCTAGTGAAGCAGTTTCTGTCGCGGGTGAAATGCTACAACAGGGCCTAAGACCCGACAAGGTTACCATGTTAGCTGCAATTTCAGCTTGCGCACAATTAAGTGATTCTCTCTCAGGTAAGTGTTGCCATGGTTATGTTCTAAGGAATGGACTAGAAGGTTGGGATACCATTTGTAATGCCATGATTGACATGTACATGAAGTGCGGCCAACAAGAGCTGGCCTGCATAATTTTTGACAATATGTCGAATAAGACTGTAGTATCATGGAACTCCTTGATTTCTGGTTTCATAAGAAGTGGCGATGTGAAGTCAGCTTGGGAAATTTTCAATGAGATGCCTAAGAGTGATCTCGTCACTTGGAACACTATGATTGGTGCTCTGGTCCAAGAGAGCAAGTTTGAGGAAGCAATTGAACTTTTCCGGGTGATGCAGACTGAGGGAGTAAAAGGAGATAGGGTGACCATGGTGGAGGTTGCATCTGCCTGCGGATATTTAGGAGCTCTTGATCTTGCAAAGTGGACTCATGCTTATATTGAAAAAAACGAAATCAAATGTGATATGCGGCTTGGGACAGCCTTAGTTGACATGTTTGCTAAATGTGGTGATCCTCAAAGTGCAATGAAAGTGTTCAATACAATGGCAAGGAGAGATGTTTCTGCTTGGACTGCAGCCATCAGAGCAATGGCCATGGAGGGAAATGGGAAACGAGCTCTAGAACTTTTTGATGACATGCTTAAGCAAGGGGTGAAACCAGATGAAGTAGTCTTTGTGGCAGTACTATCAGCGTGCAGCCATGTTGGTCTTTTGGAACAAGGGCGGAACATTTTTATGTCAATGCAGTTGGTCCATGGCATTTCCCCTCATATCGTTCATTACGGTTGCATGGTTGATCTACTAGGCCGAGCCGGGCTCTTGGAAGAAGCTGTTGATCTGATAAAGTGCATGCCAATGGAACCCAATGATGTCATTTGGGGCACTCTCTTGGCTGCTTGTCGAACCCACAAAAACGTCAAAATGGCAACATATGCAGCTGAACAGATATCAAAGTTGACCACTCAGAGGACTGGTATTCATGTGCTTCTCTCAAACATATATGCATCAGCTGGGAAATGGGCAGACGTTGCAAAAGTGAGGCTACAGTTGAAAGAGAGAGGGATTCAGAAGGTACCCGGATCCAGTTCCATTGAAGTTAATGGAGTGATTCATGAGTTTACCTCCGGTGGTGATACTGATACACACACAGAGAGGAGCCACATTGCGTTGATGTTGCAAGAAATAAACAGCAGACTCAGAGATGCTGGTCACATTCCTGATCTCAACAATGTCCTGCTTGATGTTGATGAGAAGGAGAAAGAGTACTTGCTCAGTCGACATAGTGAGAAAGTGGCCATAGCTTTTGGGCTTATAGGTACGGGGCAAGGAGTGCCAATTCGTGTGGTGAAGAATCTGAGAATGTGTTCTGATTGTCACTCTTTTGCCAAATTAGTATCAAGAATATATGACAGGGAAATTATTGTTCGAGATAACAACAGGTTCCATTTCTTTAGTCAGGGCCTGTGCTCTTGCAGTGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000234 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006665 GO:0006666 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008610 GO:0008654 GO:0008757 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016491 GO:0016651 GO:0016740 GO:0016741 GO:0019538 GO:0019637 GO:0031984 GO:0032259 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0042180 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0046519 GO:0055114 GO:0071704 GO:0090407 GO:0097164 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 ko03040 map00563 map01100 map03040

Pfam Domains

Protein Families

PIG-F

Protein Analysis

848

Amino Acids

93.6

Weight (kDa)

6.13

Isoelectric Point (pI)

43.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	106 - 152	7.5e-08	PPR repeat family
PPR	PF01535	106 - 136	8.8e-07	PPR repeat
PPR_2	PF13041	204 - 253	3.1e-12	PPR repeat family
PPR	PF01535	207 - 237	9.8e-07	PPR repeat
PPR_2	PF13041	306 - 353	6.7e-08	PPR repeat family
PPR_2	PF13041	407 - 437	1.3e-08	PPR repeat family
PPR_1	PF12854	407 - 434	4.4e-06	PPR repeat
PPR	PF01535	409 - 438	1.1e-07	PPR repeat
PPR_2	PF13041	438 - 477	7.1e-09	PPR repeat family
PPR	PF01535	440 - 470	9.8e-08	PPR repeat
PPR_2	PF13041	538 - 586	6.1e-09	PPR repeat family
E_motif	PF20431	656 - 718	3.1e-16	E motif
DYW_deaminase	PF14432	756 - 848	6.6e-39	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007942)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16040 AT1G16040 AT1G16040 AT1G16040
fragaria_vesca	FvH4_6g28300 FvH4_6g28300 FvH4_6g28300
malus_domestica	MD12G1013200.v1.1 MD14G1138800.v1.1
prunus_persica	Prupe.3G103300_v2.0.a1
pyrus_communis	pycom12g01050
rosa_chinensis	RchiOBHm_Chr2g0132361
rosa_laevigata	RLG00000019249
rosa_multiflora	Rmu_sc0000781.1_g000005 Rmu_sc0002795.1_g000001
rosa_roxburghii	Rroxscaffold_2G00112120
rosa_rugosa	Rorug02G0303900 Rorug02G0304000 Rorug02G0304100
rosa_samantha	Rh2AG357000 Rh2BG363500 Rh2CG340500 Rh2DG380200
rosa_wichuraiana	Rw2G029030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1057
Acc65I	GGTACC	1 cut(s)	2131
AccB1I	GGYRCC	2 cut(s)	233, 2131
AccB7I	CCANNNNNTGG	1 cut(s)	964
AccI	GTMKAC	1 cut(s)	2335
AccII	CGCG	2 cut(s)	446, 989
AciI	CCGC	8 cut(s)	6, 882, 889, 989, 1170, 1452, 1528, 1783
AclWI	GGATC	3 cut(s)	1565, 2133, 2146
AcoI	YGGCCR	4 cut(s)	3, 1171, 1650, 2352
AcsI	RAATTY	3 cut(s)	535, 838, 1287
AcuI	CTGAAG	1 cut(s)	383
AfaI	GTAC	5 cut(s)	1160, 1743, 2133, 2324, 2377
AfiI	CCNNNNNNNGG	4 cut(s)	108, 888, 964, 2025
AflII	CTTAAG	1 cut(s)	1700
AflIII	ACRYGT	2 cut(s)	1155, 1551
AgsI	TTSAA	8 cut(s)	284, 763, 1294, 1379, 1502, 1597, 2110, 2153
AhdI	GACNNNNNGTC	1 cut(s)	1207
AhlI	ACTAGT	1 cut(s)	629
AjnI	CCWGG	1 cut(s)	957
AjuI	GAANNNNNNNTTGG	2 cut(s)	494, 526
Alw21I	GWGCWC	5 cut(s)	452, 1345, 1468, 1678, 2528
Alw26I	GTCTC	1 cut(s)	37
AlwI	GGATC	3 cut(s)	1565, 2133, 2146
AlwNI	CAGNNNCTG	3 cut(s)	964, 983, 2521
AoxI	GGCC	9 cut(s)	3, 175, 1009, 1171, 1185, 1650, 1864, 2352, 2518
ApeKI	GCWGC	7 cut(s)	484, 701, 1040, 1634, 1755, 1952, 1994
ApoI	RAATTY	3 cut(s)	535, 838, 1287
Asp700I	GAANNNNTTC	3 cut(s)	477, 1592, 2422
Asp718I	GGTACC	1 cut(s)	2131
AspLEI	GCGC	6 cut(s)	218, 224, 446, 794, 957, 1058
AspS9I	GGNCC	4 cut(s)	1009, 1348, 1810, 2518
AsuC2I	CCSGG	4 cut(s)	259, 1388, 1873, 2136
AsuII	TTCGAA	1 cut(s)	240
AvaII	GGWCC	2 cut(s)	1348, 1810
BaeGI	GKGCMC	3 cut(s)	236, 570, 1943
BaeI	ACNNNNGTAYC	2 cut(s)	1209, 1242
BalI	TGGCCA	2 cut(s)	1652, 2354
BamHI	GGATCC	1 cut(s)	2138
BanI	GGYRCC	2 cut(s)	233, 2131
BanII	GRGCYC	4 cut(s)	381, 1468, 1678, 1878
Bbv12I	GWGCWC	5 cut(s)	452, 1345, 1468, 1678, 2528
BbvI	GCAGC	7 cut(s)	471, 688, 1027, 1646, 1767, 1939, 2006
BccI	CCATC	4 cut(s)	385, 499, 688, 1646
BciT130I	CCWGG	1 cut(s)	959
BciVI	GTATCC	1 cut(s)	1123
BcnI	CCSGG	4 cut(s)	259, 1388, 1873, 2136
BcoDI	GTCTC	1 cut(s)	37
BcuI	ACTAGT	1 cut(s)	629
BfaI	CTAG	6 cut(s)	348, 630, 969, 1118, 1679, 1862
BfmI	CTRYAG	3 cut(s)	1221, 1632, 2102
BfrI	CTTAAG	1 cut(s)	1700
BfuI	GTATCC	1 cut(s)	1123
BmcAI	AGTACT	2 cut(s)	1743, 2324
Bme1390I	CCNGG	5 cut(s)	259, 959, 1388, 1873, 2136
Bme18I	GGWCC	2 cut(s)	1348, 1810
BmeRI	GACNNNNNGTC	1 cut(s)	1207
BmgT120I	GGNCC	4 cut(s)	1009, 1348, 1810, 2518
BmiI	GGNNCC	6 cut(s)	235, 1920, 2133, 2140, 2216, 2501
BmrFI	CCNGG	5 cut(s)	259, 959, 1388, 1873, 2136
BmrI	ACTGGG	1 cut(s)	117
BmsI	GCATC	6 cut(s)	868, 1290, 1383, 1451, 2072, 2251
BmuI	ACTGGG	1 cut(s)	117
BplI	GAGNNNNNCTC	4 cut(s)	1860, 1892, 2165, 2197
Bpu14I	TTCGAA	1 cut(s)	240
BpuEI	CTTGAG	2 cut(s)	626, 800
BpuMI	CCSGG	4 cut(s)	259, 1388, 1873, 2136
BsaI	GGTCTC	1 cut(s)	37
BsaJI	CCNNGG	6 cut(s)	90, 958, 1093, 1428, 1653, 1813
BsaWI	WCCGGW	1 cut(s)	2183
BsaXI	ACNNNNNCTCC	4 cut(s)	1407, 1437, 2306, 2336
Bsc4I	CCNNNNNNNGG	4 cut(s)	108, 888, 964, 2025
Bse118I	RCCGGY	1 cut(s)	704
Bse1I	ACTGG	5 cut(s)	112, 969, 2035, 2142, 2546
Bse3DI	GCAATG	4 cut(s)	15, 1590, 1653, 2220
BseBI	CCWGG	1 cut(s)	959
BseDI	CCNNGG	6 cut(s)	90, 958, 1093, 1428, 1653, 1813
BseGI	GGATG	1 cut(s)	772
BseLI	CCNNNNNNNGG	4 cut(s)	108, 888, 964, 2025
BseMI	GCAATG	4 cut(s)	15, 1590, 1653, 2220
BseNI	ACTGG	5 cut(s)	112, 969, 2035, 2142, 2546
BseRI	GAGGAG	2 cut(s)	380, 2227
BseSI	GKGCMC	3 cut(s)	236, 570, 1943
BseXI	GCAGC	7 cut(s)	471, 688, 1027, 1646, 1767, 1939, 2006
BseYI	CCCAGC	2 cut(s)	353, 2069
BsgI	GTGCAG	2 cut(s)	470, 1774
Bsh1236I	CGCG	2 cut(s)	446, 989
BshFI	GGCC	9 cut(s)	5, 177, 1011, 1173, 1187, 1652, 1866, 2354, 2520
BshNI	GGYRCC	2 cut(s)	233, 2131
BsiHKAI	GWGCWC	5 cut(s)	452, 1345, 1468, 1678, 2528
BsiSI	CCGG	7 cut(s)	259, 413, 705, 1387, 1872, 2136, 2184
BslFI	GGGAC	2 cut(s)	975, 1549
BslI	CCNNNNNNNGG	4 cut(s)	108, 888, 964, 2025
BsmAI	GTCTC	1 cut(s)	37
BsmFI	GGGAC	2 cut(s)	975, 1549
BsnI	GGCC	9 cut(s)	5, 177, 1011, 1173, 1187, 1652, 1866, 2354, 2520
Bso31I	GGTCTC	1 cut(s)	37
Bsp119I	TTCGAA	1 cut(s)	240
Bsp1407I	TGTACA	1 cut(s)	1158
Bsp143I	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
Bsp19I	CCATGG	4 cut(s)	1093, 1428, 1653, 1813
BspACI	CCGC	8 cut(s)	6, 882, 889, 989, 1170, 1452, 1528, 1783
BspANI	GGCC	9 cut(s)	5, 177, 1011, 1173, 1187, 1652, 1866, 2354, 2520
BspFNI	CGCG	2 cut(s)	446, 989
BspHI	TCATGA	1 cut(s)	2170
BspLI	GGNNCC	6 cut(s)	235, 1920, 2133, 2140, 2216, 2501
BspMAI	CTGCAG	1 cut(s)	1636
BspPI	GGATC	3 cut(s)	1565, 2133, 2146
BspT104I	TTCGAA	1 cut(s)	240
BspT107I	GGYRCC	2 cut(s)	233, 2131
BspTI	CTTAAG	1 cut(s)	1700
BspTNI	GGTCTC	1 cut(s)	37
BsrDI	GCAATG	4 cut(s)	15, 1590, 1653, 2220
BsrFI	RCCGGY	1 cut(s)	704
BsrGI	TGTACA	1 cut(s)	1158
BsrI	ACTGG	5 cut(s)	112, 969, 2035, 2142, 2546
BssAI	RCCGGY	1 cut(s)	704
BssECI	CCNNGG	6 cut(s)	90, 958, 1093, 1428, 1653, 1813
BssMI	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
BssT1I	CCWWGG	5 cut(s)	90, 1093, 1428, 1653, 1813
Bst2UI	CCWGG	1 cut(s)	959
Bst4CI	ACNGT	5 cut(s)	125, 158, 1222, 1844, 2106
BstAFI	CTTAAG	1 cut(s)	1700
BstAPI	GCANNNNNTGC	3 cut(s)	1991, 2087, 2389
BstAUI	TGTACA	1 cut(s)	1158
BstBI	TTCGAA	1 cut(s)	240
BstC8I	GCNNGC	9 cut(s)	220, 448, 672, 1054, 1185, 1189, 1450, 1753, 1910
BstDSI	CCRYGG	4 cut(s)	1093, 1428, 1653, 1813
BstEII	GGTNACC	2 cut(s)	1027, 1423
BstF5I	GGATG	1 cut(s)	772
BstFNI	CGCG	2 cut(s)	446, 989
BstHHI	GCGC	6 cut(s)	218, 224, 446, 794, 957, 1058
BstKTI	GATC	7 cut(s)	1315, 1474, 1573, 1858, 1897, 2141, 2281
BstMAI	GTCTC	1 cut(s)	37
BstMBI	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
BstNI	CCWGG	1 cut(s)	959
BstNSI	RCATGY	5 cut(s)	222, 1159, 1555, 1699, 1912
BstPI	GGTNACC	2 cut(s)	1027, 1423
BstSCI	CCNGG	5 cut(s)	257, 957, 1386, 1871, 2134
BstSFI	CTRYAG	3 cut(s)	1221, 1632, 2102
BstSLI	GKGCMC	3 cut(s)	236, 570, 1943
BstUI	CGCG	2 cut(s)	446, 989
BstV1I	GCAGC	7 cut(s)	471, 688, 1027, 1646, 1767, 1939, 2006
BstX2I	RGATCY	1 cut(s)	2138
BstYI	RGATCY	1 cut(s)	2138
BsuI	GTATCC	1 cut(s)	1123
BsuRI	GGCC	9 cut(s)	5, 177, 1011, 1173, 1187, 1652, 1866, 2354, 2520
BtgI	CCRYGG	4 cut(s)	1093, 1428, 1653, 1813
BtgZI	GCGATG	1 cut(s)	1281
BtsCI	GGATG	1 cut(s)	772
BtsI	GCAGTG	1 cut(s)	2539
BtsIMutI	CAGTG	3 cut(s)	154, 437, 2539
Cac8I	GCNNGC	9 cut(s)	220, 448, 672, 1054, 1185, 1189, 1450, 1753, 1910
CaiI	CAGNNNCTG	3 cut(s)	964, 983, 2521
CciI	TCATGA	1 cut(s)	2170
CfoI	GCGC	6 cut(s)	218, 224, 446, 794, 957, 1058
Cfr10I	RCCGGY	1 cut(s)	704
Cfr13I	GGNCC	4 cut(s)	1009, 1348, 1810, 2518
Csp6I	GTAC	5 cut(s)	1159, 1742, 2132, 2323, 2376
CspCI	CAANNNNNGTGG	4 cut(s)	441, 476, 2382, 2417
CviQI	GTAC	5 cut(s)	1159, 1742, 2132, 2323, 2376
DpnI	GATC	7 cut(s)	1314, 1473, 1572, 1857, 1896, 2140, 2280
DpnII	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
DriI	GACNNNNNGTC	1 cut(s)	1207
EaeI	YGGCCR	4 cut(s)	3, 1171, 1650, 2352
Eam1105I	GACNNNNNGTC	1 cut(s)	1207
EciI	GGCGGA	1 cut(s)	1798
Ecl136II	GAGCTC	2 cut(s)	1466, 1676
Eco130I	CCWWGG	5 cut(s)	90, 1093, 1428, 1653, 1813
Eco147I	AGGCCT	1 cut(s)	177
Eco24I	GRGCYC	4 cut(s)	381, 1468, 1678, 1878
Eco31I	GGTCTC	1 cut(s)	37
Eco32I	GATATC	1 cut(s)	2007
Eco47I	GGWCC	2 cut(s)	1348, 1810
Eco53kI	GAGCTC	2 cut(s)	1466, 1676
Eco57I	CTGAAG	1 cut(s)	383
Eco91I	GGTNACC	2 cut(s)	1027, 1423
EcoICRI	GAGCTC	2 cut(s)	1466, 1676
EcoO109I	RGGNCCY	2 cut(s)	1009, 2518
EcoO65I	GGTNACC	2 cut(s)	1027, 1423
EcoRI	GAATTC	2 cut(s)	535, 838
EcoRII	CCWGG	1 cut(s)	957
EcoRV	GATATC	1 cut(s)	2007
EcoT14I	CCWWGG	5 cut(s)	90, 1093, 1428, 1653, 1813
EcoT22I	ATGCAT	1 cut(s)	2065
EcoT38I	GRGCYC	4 cut(s)	381, 1468, 1678, 1878
ErhI	CCWWGG	5 cut(s)	90, 1093, 1428, 1653, 1813
FaqI	GGGAC	2 cut(s)	975, 1549
FauI	CCCGC	1 cut(s)	982
FauNDI	CATATG	1 cut(s)	1990
FblI	GTMKAC	1 cut(s)	2335
FokI	GGATG	1 cut(s)	779
FriOI	GRGCYC	4 cut(s)	381, 1468, 1678, 1878
FspBI	CTAG	6 cut(s)	348, 630, 969, 1118, 1679, 1862
FspI	TGCGCA	1 cut(s)	1057
GlaI	GCGC	6 cut(s)	217, 223, 445, 793, 956, 1057
GsaI	CCCAGC	2 cut(s)	357, 2073
HaeIII	GGCC	9 cut(s)	5, 177, 1011, 1173, 1187, 1652, 1866, 2354, 2520
HapII	CCGG	7 cut(s)	259, 413, 705, 1387, 1872, 2136, 2184
HhaI	GCGC	6 cut(s)	218, 224, 446, 794, 957, 1058
Hin6I	GCGC	6 cut(s)	216, 222, 444, 792, 955, 1056
HinP1I	GCGC	6 cut(s)	216, 222, 444, 792, 955, 1056
HincII	GTYRAC	3 cut(s)	1549, 2016, 2336
HindII	GTYRAC	3 cut(s)	1549, 2016, 2336
HindIII	AAGCTT	1 cut(s)	244
HpaII	CCGG	7 cut(s)	259, 413, 705, 1387, 1872, 2136, 2184
Hpy166II	GTNNAC	7 cut(s)	423, 627, 1485, 1549, 2016, 2178, 2336
Hpy188I	TCNGA	8 cut(s)	390, 1643, 1899, 2025, 2127, 2258, 2415, 2428
Hpy8I	GTNNAC	7 cut(s)	423, 627, 1485, 1549, 2016, 2178, 2336
HpyAV	CCTTC	7 cut(s)	247, 467, 580, 757, 1115, 2122, 2305
HpyCH4III	ACNGT	5 cut(s)	125, 158, 1222, 1844, 2106
HpyCH4IV	ACGT	2 cut(s)	1974, 2085
HpySE526I	ACGT	2 cut(s)	1974, 2085
HspAI	GCGC	6 cut(s)	216, 222, 444, 792, 955, 1056
KpnI	GGTACC	1 cut(s)	2135
Kzo9I	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
LmnI	GCTCC	3 cut(s)	61, 1463, 2214
Lsp1109I	GCAGC	7 cut(s)	471, 688, 1027, 1646, 1767, 1939, 2006
LweI	GCATC	6 cut(s)	868, 1290, 1383, 1451, 2072, 2251
MaeI	CTAG	6 cut(s)	348, 630, 969, 1118, 1679, 1862
MaeII	ACGT	2 cut(s)	1974, 2085
MalI	GATC	7 cut(s)	1314, 1473, 1572, 1857, 1896, 2140, 2280
MboI	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
MboII	GAAGA	9 cut(s)	296, 299, 302, 305, 308, 514, 530, 1895, 2419
MfeI	CAATTG	1 cut(s)	1374
MflI	RGATCY	1 cut(s)	2138
MlsI	TGGCCA	2 cut(s)	1652, 2354
MluNI	TGGCCA	2 cut(s)	1652, 2354
MlyI	GAGTC	5 cut(s)	31, 807, 815, 1480, 2247
Mox20I	TGGCCA	2 cut(s)	1652, 2354
Mph1103I	ATGCAT	1 cut(s)	2065
MroXI	GAANNNNTTC	3 cut(s)	477, 1592, 2422
MscI	TGGCCA	2 cut(s)	1652, 2354
MseI	TTAA	5 cut(s)	270, 711, 1064, 1701, 2157
MslI	CAYNNNNRTG	5 cut(s)	552, 1098, 1160, 1793, 1913
Msp20I	TGGCCA	2 cut(s)	1652, 2354
MspA1I	CMGCKG	4 cut(s)	19, 484, 1997, 2069
MspCI	CTTAAG	1 cut(s)	1700
MspI	CCGG	7 cut(s)	259, 413, 705, 1387, 1872, 2136, 2184
MspR9I	CCNGG	5 cut(s)	259, 959, 1388, 1873, 2136
MunI	CAATTG	1 cut(s)	1374
MvaI	CCWGG	1 cut(s)	959
MvnI	CGCG	2 cut(s)	446, 989
NciI	CCSGG	4 cut(s)	259, 1388, 1873, 2136
NcoI	CCATGG	4 cut(s)	1093, 1428, 1653, 1813
NdeI	CATATG	1 cut(s)	1990
NdeII	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
NlaIV	GGNNCC	6 cut(s)	235, 1920, 2133, 2140, 2216, 2501
NmeAIII	GCCGAG	1 cut(s)	1892
NmuCI	GTSAC	9 cut(s)	111, 158, 182, 199, 724, 1318, 1423, 2267, 2432
NsbI	TGCGCA	1 cut(s)	1057
NsiI	ATGCAT	1 cut(s)	2065
NspI	RCATGY	5 cut(s)	222, 1159, 1555, 1699, 1912
NspV	TTCGAA	1 cut(s)	240
PaeI	GCATGC	2 cut(s)	222, 1912
PagI	TCATGA	1 cut(s)	2170
PceI	AGGCCT	1 cut(s)	177
PciI	ACATGT	2 cut(s)	1155, 1551
PdmI	GAANNNNTTC	3 cut(s)	477, 1592, 2422
PfeI	GAWTC	5 cut(s)	544, 1070, 2122, 2167, 2410
PflMI	CCANNNNNTGG	1 cut(s)	964
PleI	GAGTC	5 cut(s)	30, 807, 814, 1480, 2247
PpsI	GAGTC	5 cut(s)	30, 807, 814, 1480, 2247
PscI	ACATGT	2 cut(s)	1155, 1551
Psp124BI	GAGCTC	2 cut(s)	1468, 1678
Psp6I	CCWGG	1 cut(s)	957
PspEI	GGTNACC	2 cut(s)	1027, 1423
PspFI	CCCAGC	2 cut(s)	353, 2069
PspGI	CCWGG	1 cut(s)	957
PspN4I	GGNNCC	6 cut(s)	235, 1920, 2133, 2140, 2216, 2501
PspPI	GGNCC	4 cut(s)	1009, 1348, 1810, 2518
PstI	CTGCAG	1 cut(s)	1636
PstNI	CAGNNNCTG	3 cut(s)	964, 983, 2521
PsuI	RGATCY	1 cut(s)	2138
PvuII	CAGCTG	4 cut(s)	19, 484, 1997, 2069
RsaI	GTAC	5 cut(s)	1160, 1743, 2133, 2324, 2377
RsaNI	GTAC	5 cut(s)	1159, 1742, 2132, 2323, 2376
RseI	CAYNNNNRTG	5 cut(s)	552, 1098, 1160, 1793, 1913
SacI	GAGCTC	2 cut(s)	1468, 1678
SalI	GTCGAC	1 cut(s)	2334
SaqAI	TTAA	5 cut(s)	270, 711, 1064, 1701, 2157
Sau3AI	GATC	7 cut(s)	1312, 1471, 1570, 1855, 1894, 2138, 2278
Sau96I	GGNCC	4 cut(s)	1009, 1348, 1810, 2518
ScaI	AGTACT	2 cut(s)	1743, 2324
SchI	GAGTC	5 cut(s)	31, 807, 815, 1480, 2247
ScrFI	CCNGG	5 cut(s)	259, 959, 1388, 1873, 2136
SfaNI	GCATC	6 cut(s)	868, 1290, 1383, 1451, 2072, 2251
SfcI	CTRYAG	3 cut(s)	1221, 1632, 2102
SfuI	TTCGAA	1 cut(s)	240
SinI	GGWCC	2 cut(s)	1348, 1810
SmiMI	CAYNNNNRTG	5 cut(s)	552, 1098, 1160, 1793, 1913
SmlI	CTYRAG	3 cut(s)	605, 815, 1700
SmoI	CTYRAG	3 cut(s)	605, 815, 1700
SpeI	ACTAGT	1 cut(s)	629
SphI	GCATGC	2 cut(s)	222, 1912
SseBI	AGGCCT	1 cut(s)	177
SsiI	CCGC	8 cut(s)	6, 882, 889, 989, 1170, 1452, 1528, 1783
SspMI	CTAG	6 cut(s)	348, 630, 969, 1118, 1679, 1862
SstI	GAGCTC	2 cut(s)	1468, 1678
StuI	AGGCCT	1 cut(s)	177
StyD4I	CCNGG	5 cut(s)	257, 957, 1386, 1871, 2134
StyI	CCWWGG	5 cut(s)	90, 1093, 1428, 1653, 1813
TaaI	ACNGT	5 cut(s)	125, 158, 1222, 1844, 2106
TaiI	ACGT	2 cut(s)	1977, 2088
TaqI	TCGA	6 cut(s)	240, 690, 1211, 1960, 2335, 2485
TatI	WGTACW	3 cut(s)	1158, 1741, 2322
TauI	GCSGC	5 cut(s)	8, 884, 892, 1173, 1531
TfiI	GAWTC	5 cut(s)	544, 1070, 2122, 2167, 2410
Tru1I	TTAA	5 cut(s)	270, 711, 1064, 1701, 2157
Tru9I	TTAA	5 cut(s)	270, 711, 1064, 1701, 2157
TscAI	CASTG	3 cut(s)	161, 444, 2539
TseFI	GTSAC	9 cut(s)	111, 158, 182, 199, 724, 1318, 1423, 2267, 2432
TseI	GCWGC	7 cut(s)	484, 701, 1040, 1634, 1755, 1952, 1994
Tsp45I	GTSAC	9 cut(s)	111, 158, 182, 199, 724, 1318, 1423, 2267, 2432
TspGWI	ACGGA	1 cut(s)	420
TspRI	CASTG	3 cut(s)	161, 444, 2539
Van91I	CCANNNNNTGG	1 cut(s)	964
Vha464I	CTTAAG	1 cut(s)	1700
VpaK11BI	GGWCC	2 cut(s)	1348, 1810
XapI	RAATTY	3 cut(s)	535, 838, 1287
XbaI	TCTAGA	1 cut(s)	1678
XceI	RCATGY	5 cut(s)	222, 1159, 1555, 1699, 1912
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1645
XmiI	GTMKAC	1 cut(s)	2335
XmnI	GAANNNNTTC	3 cut(s)	477, 1592, 2422
XspI	CTAG	6 cut(s)	348, 630, 969, 1118, 1679, 1862
ZrmI	AGTACT	2 cut(s)	1743, 2324
Zsp2I	ATGCAT	1 cut(s)	2065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.