Rorug02G0346600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010176
Physical Location & Seq
Reverse (-)
43321696 .. 43321995
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0346600.1

Sequence Viewer

Length: 300 bp
ATGGTGGCTGGTGGTGGGAGGCCGTTGGCAGGTTTAATTTCACCAGAACATGCAGAGGTTTTGGCATGTAGGATGGCAATTGAGTTTGCCTTGGCTCAGAATTTGTTACCTGCTGTATTAGAAACTGATTCTGAGATGGTGCAACAACAACTATCTAGCTCTGAAGGGTCTAACACTTCCTTTCTGGGTCGTATCTATGAGGATCTGACCTCCATATTGGCTTCAACTCCCAATTTGCAGATCAGTCACACTAGAAGAGATGCCAACAAAGGTCGCACACCTAATGGCTGCGCACGCTAG

Protein Analysis

99

Amino Acids

10.55

Weight (kDa)

5.57

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 4 - 90 1.5e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 292
Acc36I ACCTGC 2 cut(s) 20, 118
AclWI GGATC 1 cut(s) 210
AcsI RAATTY 1 cut(s) 100
AcuI CTGAAG 1 cut(s) 183
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 225
AluBI AGCT 1 cut(s) 159
AluI AGCT 1 cut(s) 159
AlwI GGATC 1 cut(s) 210
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 288
ApoI RAATTY 1 cut(s) 100
AspLEI GCGC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 33
BbvI GCAGC 1 cut(s) 275
BccI CCATC 2 cut(s) 67, 130
BceAI ACGGC 1 cut(s) 7
BfaI CTAG 3 cut(s) 156, 252, 298
BfuAI ACCTGC 2 cut(s) 20, 118
BisI GCNGC 1 cut(s) 289
BlsI GCNGC 1 cut(s) 290
BmsI GCATC 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMII CTCAG 2 cut(s) 110, 123
BseXI GCAGC 1 cut(s) 275
BshFI GGCC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 29
BsnI GGCC 1 cut(s) 22
Bsp143I GATC 2 cut(s) 202, 240
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 2 cut(s) 109, 124
BspMI ACCTGC 2 cut(s) 20, 118
BspPI GGATC 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 2 cut(s) 202, 240
BssT1I CCWWGG 1 cut(s) 90
Bst6I CTCTTC 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 96, 132
BstF5I GGATG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 205, 243
BstMBI GATC 2 cut(s) 202, 240
BstMWI GCNNNNNNNGC 1 cut(s) 294
BstNSI RCATGY 2 cut(s) 53, 69
BstV1I GCAGC 1 cut(s) 275
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 78
BveI ACCTGC 2 cut(s) 20, 118
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 1 cut(s) 293
CviAII CATG 2 cut(s) 50, 66
CviJI RGCY 6 cut(s) 8, 22, 95, 159, 221, 288
CviKI_1 RGCY 6 cut(s) 8, 22, 95, 159, 221, 288
DdeI CTNAG 2 cut(s) 96, 132
DpnI GATC 2 cut(s) 204, 242
DpnII GATC 2 cut(s) 202, 240
Eam1104I CTCTTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 53, 69
FaiI YATR 4 cut(s) 51, 67, 198, 215
FatI CATG 2 cut(s) 49, 65
Fnu4HI GCNGC 1 cut(s) 289
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 1 cut(s) 289
FspBI CTAG 3 cut(s) 156, 252, 298
FspI TGCGCA 1 cut(s) 292
GlaI GCGC 1 cut(s) 292
GluI GCNGC 1 cut(s) 289
HaeIII GGCC 1 cut(s) 22
HhaI GCGC 1 cut(s) 293
Hin1II CATG 2 cut(s) 53, 69
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 1 cut(s) 128
HphI GGTGA 1 cut(s) 33
Hpy188I TCNGA 4 cut(s) 99, 133, 163, 207
HpyAV CCTTC 1 cut(s) 158
HpyCH4V TGCA 3 cut(s) 53, 142, 238
HpyF10VI GCNNNNNNNGC 1 cut(s) 294
HpyF3I CTNAG 2 cut(s) 96, 132
Hsp92II CATG 2 cut(s) 53, 69
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 202, 240
LpnPI CCDG 4 cut(s) 15, 57, 123, 170
Lsp1109I GCAGC 1 cut(s) 275
LweI GCATC 1 cut(s) 250
MaeI CTAG 3 cut(s) 156, 252, 298
MaeIII GTNAC 2 cut(s) 105, 245
MalI GATC 2 cut(s) 204, 242
MboI GATC 2 cut(s) 202, 240
MboII GAAGA 1 cut(s) 267
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 1 cut(s) 202
MluCI AATT 4 cut(s) 36, 78, 100, 232
MnlI CCTC 4 cut(s) 12, 49, 193, 220
MseI TTAA 1 cut(s) 35
MunI CAATTG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 294
NdeII GATC 2 cut(s) 202, 240
NlaIII CATG 2 cut(s) 53, 69
NmuCI GTSAC 1 cut(s) 245
NsbI TGCGCA 1 cut(s) 292
NspI RCATGY 2 cut(s) 53, 69
PfeI GAWTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 290
PsuI RGATCY 1 cut(s) 202
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 289
Sau3AI GATC 2 cut(s) 202, 240
SetI ASST 7 cut(s) 34, 60, 112, 161, 212, 274, 283
SfaNI GCATC 1 cut(s) 250
Sse9I AATT 4 cut(s) 36, 78, 100, 232
SspMI CTAG 3 cut(s) 156, 252, 298
StyI CCWWGG 1 cut(s) 90
TasI AATT 4 cut(s) 36, 78, 100, 232
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 1 cut(s) 245
XapI RAATTY 1 cut(s) 100
XceI RCATGY 2 cut(s) 53, 69
XspI CTAG 3 cut(s) 156, 252, 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.