Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0010176

Physical Location & Seq

Genomic Coordinates Reverse (-)

58916686 .. 58919387

Gene Structure 2702 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG393400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGCCAAATTGATTGCCAAGTCGGTTGCTTCAACTCGATCGATATCAACCTCCACTCATCTCTCTCAGAGACTTAATCAAACAGAAAATGAGATAGTTAAGATGTTCCGGTTGCCTAATGCATCGGACAGCTTTCCCATGAGTAGAAATATGCCAGGGAAGGACCCTTCAGTCAGGACATTGGATGACAGGTTCACAAAGATTTTGAAAATTTTCAAGTGGGGGCCTGATGCCGAAAAGGCCTTGGAAGTGCTGAAGCTGAGAGTGGATCATAGGTTGGTTCTCGCGGTTCTCAAGATTGATGTCGAGGCCATTGTTAAGGTTAAGTTTTTCAAGTGGGTTGGGAAGAGAAGGAATTTCCAACACAATTCAACCACATATATGGCGTTGATCCGTTGCTTGGACGAGGCTGGTATGGTTGGTGAAATGTGGAAAGCCATTCAGGAGATGGTTCGAAGTGCACGTGTTATCGATCCAGCTGAATTGTCTGAGATTATAAGAATCTTGGGAAGGGCTAAGATGGTAAACAAGGCACTATCTGTCTTCTACCAAATCAAAGGCCACAACTGCATGCCAACAGCTCATAGTTACAACTCCATGATCTTAATGCTGATGCAAGAAGGTCATCATGAAAAGGTCCATGAGCTCTATAATGAGATGTGTAATGAGAGTAACTGTTTCCCGGACACAGTCACATACAGTGCACTAATATCTGCATTTGGGAAATTGGGTCGTGATGATTCTGCAATTAGGTTGTTTGATGAGATGAAGGATAATGGTTTACATCCAACTGCAAAGATTTATACTACCCTATTGGCAATCTTTTTCAAGTTGGGCAAGGTTGAGAAAGCTTTAAGTCTAGTTCAGGAGATGAAAGAGAATGGGTGTCCGCCTACTGTTTTTACTTACACTGAATTGATAAAGGGGCTAGGGAAAGCTGGGAGGGTTGAAGATGCTTATGTCATATACAAAAACATGCTGAAGGAGGGTTGTAAGCCTGATGTTGTGCTCATGAACAATTTGATAAACATTTTGGGAAAGGAAGGTCGCATAGAAAATGCTATTAATCTTTTCAATGAGATGGACATTTTGCGTTGCACACCTAATGTGGTGACTTATAACACGATAATTAAAGCTTTATTTGATTCCAAAGCTCCAGCTTCGGTTGCAGCTTCATGGTTTGAGAAGATGAAAGTGAATGGTGTTATTCCGAGCTCTTTTACCTATTCGATTCTGATAGATGGTTTTTGCAAAACAAATAGAGTTGAGAAAGCTTTGTTGCTTCTTGAAGAGATGGATGAAAAGGGGTTCCCCCCTTGTCCAGCTGCGTATTGCAGCCTGATCAACAGTCTTGGAAAAGCAAAGCGGTATGAAGCTGCAAATGAGTTATTTCAAGAATTGAAAGAAAACTGTGGACGTGCAAGTGCTAGAGTATATGCCGTGATGATTAAACATTTCGGAAAATGTGGGCGTCTTGATGAAGCTCTGGATCTTTTTCACGAGATGAAGAAACTTGGATGCAATCCGGATGTATATACTTATAATGCCCTCATGTCAGGGATGGTCAGGGCTGGTATGATAGATGAGGCACTTTCCTTGCTTAAAACCATGGAAGAAAGGGGCTGTGTTGCAGACATAAACTCACAAAACATCATTTTAAATGGCTTGGCTAGGGCAGGTGGCCCAAAGAGGGCTTTAGAAATGTTTTCAAAGATGAAGCATTCCAAGATTCAACCAGATGCAGTTTCTTATAACACTATTCTTGGTTGTCTCAGTCGAGCTGGCTTGCTTGAAGAGGCTGCAAAGCTGATGAAAGAGATGACTTCAAAAGGTTTTCAATATGATCTCATCACCTACTCATCAATACTTGAGGCAGTTGGCAAGGTTGATGAAGCTCATATGACTTCCTTAAGGGGATCTGGAAACACGCTCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

645

Amino Acids

72.19

Weight (kDa)

8.9

Isoelectric Point (pI)

28.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	174 - 283	1.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	192 - 242	3.9e-12	PPR repeat family
PPR_1	PF12854	225 - 257	4.3e-10	PPR repeat
PPR_2	PF13041	228 - 271	9.4e-10	PPR repeat family
TPR_24	PF23276	230 - 326	5.1e-06	Fungal tetratrico peptide repeats
PPR	PF01535	231 - 261	6e-08	PPR repeat
PPR_long	PF17177	248 - 350	3.6e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	251 - 312	7.1e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	263 - 312	4.2e-14	PPR repeat family
PPR	PF01535	267 - 296	1.4e-06	PPR repeat
PPR_1	PF12854	294 - 326	3.2e-08	PPR repeat
PPR_2	PF13041	300 - 336	7.1e-09	PPR repeat family
PPR_3	PF13812	322 - 380	1.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	330 - 361	5.2e-07	PPR repeat
PPR_2	PF13041	333 - 381	3.8e-15	PPR repeat family
PPR_3	PF13812	357 - 415	1.2e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	388 - 501	1.1e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	390 - 418	5.2e-08	PPR repeat family
PPR_1	PF12854	400 - 432	5.7e-11	PPR repeat
PPR	PF01535	407 - 437	2.9e-08	PPR repeat
PPR_2	PF13041	412 - 451	7.7e-07	PPR repeat family
PPR	PF01535	478 - 507	2.3e-07	PPR repeat
PPR_2	PF13041	478 - 521	2.8e-15	PPR repeat family
PPR_3	PF13812	498 - 554	5.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	506 - 538	8.3e-10	PPR repeat
PPR_2	PF13041	511 - 558	1.2e-11	PPR repeat family
PPR	PF01535	512 - 542	1.3e-08	PPR repeat
PPR_3	PF13812	532 - 590	3.7e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	546 - 592	4.9e-10	PPR repeat family
PPR_1	PF12854	577 - 608	1.6e-07	PPR repeat
PPR_2	PF13041	579 - 625	8.3e-12	PPR repeat family
PPR	PF01535	582 - 612	4.3e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0010176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16010
fragaria_vesca	FvH4_6g31160
malus_domestica	MD17G1195500.v1.1
prunus_persica	Prupe.3G071400_v2.0.a1
pyrus_communis	pycom17g20110
rosa_chinensis	RchiOBHm_Chr2g0137841
rosa_laevigata	RLG00000019680
rosa_multiflora	Rmu_co8002616.1_g000001 Rmu_sc0002170.1_g000007 Rmu_sc0007548.1_g000006
rosa_roxburghii	Rroxscaffold_2G00106770
rosa_rugosa	Rorug02G0346600 Rorug02G0346700 Rorug02G0346800 Rorug02G0346900 Rorug02G0347000
rosa_samantha	Rh2AG393400 Rh2BG400900 Rh2CG380100 Rh2DG415900
rosa_wichuraiana	Rw2G032220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	497, 1119, 1542, 1752
AarI	CACCTGC	1 cut(s)	1667
AasI	GACNNNNNNGTC	1 cut(s)	170
Acc36I	ACCTGC	1 cut(s)	1667
AccII	CGCG	1 cut(s)	287
AccIII	TCCGGA	1 cut(s)	1525
AciI	CCGC	3 cut(s)	287, 890, 1366
AclWI	GGATC	5 cut(s)	276, 385, 467, 1497, 1924
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	210, 355
AcuI	CTGAAG	3 cut(s)	153, 275, 1001
AcvI	CACGTG	1 cut(s)	464
AcyI	GRCGYC	1 cut(s)	1471
AfiI	CCNNNNNNNGG	2 cut(s)	400, 1690
AflII	CTTAAG	1 cut(s)	1909
AflIII	ACRYGT	1 cut(s)	463
AjiI	CACGTC	1 cut(s)	1418
AjnI	CCWGG	1 cut(s)	154
AloI	GAACNNNNNNTCC	2 cut(s)	176, 208
Alw21I	GWGCWC	5 cut(s)	463, 648, 706, 1011, 1217
Alw26I	GTCTC	2 cut(s)	64, 1775
Alw44I	GTGCAC	2 cut(s)	459, 702
AlwI	GGATC	5 cut(s)	276, 385, 467, 1497, 1924
Aor13HI	TCCGGA	1 cut(s)	1525
AoxI	GGCC	6 cut(s)	3, 224, 240, 309, 559, 1681
ApaLI	GTGCAC	2 cut(s)	459, 702
ApeKI	GCWGC	5 cut(s)	1169, 1325, 1335, 1376, 1799
ApoI	RAATTY	2 cut(s)	210, 355
AseI	ATTAAT	1 cut(s)	1065
Asp700I	GAANNNNTTC	1 cut(s)	212
AspS9I	GGNCC	4 cut(s)	163, 224, 637, 1682
AsuC2I	CCSGG	1 cut(s)	683
AsuHPI	GGTGA	3 cut(s)	434, 1123, 1843
AsuII	TTCGAA	1 cut(s)	454
AvaII	GGWCC	2 cut(s)	163, 637
BaeGI	GKGCMC	2 cut(s)	463, 706
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	2 cut(s)	648, 1217
BauI	CACGAG	1 cut(s)	1499
BbrPI	CACGTG	1 cut(s)	464
BbsI	GAAGAC	1 cut(s)	535
Bbv12I	GWGCWC	5 cut(s)	463, 648, 706, 1011, 1217
BbvI	GCAGC	5 cut(s)	1181, 1312, 1347, 1363, 1786
BccI	CCATC	6 cut(s)	442, 514, 1075, 1235, 1288, 1555
BceAI	ACGGC	1 cut(s)	1424
BciT130I	CCWGG	1 cut(s)	156
BclI	TGATCA	1 cut(s)	1341
BcnI	CCSGG	1 cut(s)	683
BcoDI	GTCTC	2 cut(s)	64, 1775
BfaI	CTAG	4 cut(s)	860, 929, 1428, 1671
BfrI	CTTAAG	1 cut(s)	1909
BfuAI	ACCTGC	1 cut(s)	1667
BglI	GCCNNNNNGGC	1 cut(s)	239
BisI	GCNGC	5 cut(s)	1170, 1326, 1336, 1377, 1800
BlsI	GCNGC	5 cut(s)	1171, 1327, 1337, 1378, 1801
Bme1390I	CCNGG	2 cut(s)	156, 683
Bme18I	GGWCC	2 cut(s)	163, 637
BmgBI	CACGTC	1 cut(s)	1418
BmgT120I	GGNCC	4 cut(s)	163, 224, 637, 1682
BmiI	GGNNCC	3 cut(s)	165, 225, 1310
BmrFI	CCNGG	2 cut(s)	156, 683
BmsI	GCATC	6 cut(s)	131, 220, 603, 943, 1508, 1729
BpiI	GAAGAC	1 cut(s)	535
BpmI	CTGGAG	1 cut(s)	1140
Bpu14I	TTCGAA	1 cut(s)	454
BpuEI	CTTGAG	2 cut(s)	278, 1889
BpuMI	CCSGG	1 cut(s)	683
Bsa29I	ATCGAT	2 cut(s)	41, 471
BsaAI	YACGTR	1 cut(s)	464
BsaBI	GATNNNNATC	1 cut(s)	43
BsaHI	GRCGYC	1 cut(s)	1471
BsaJI	CCNNGG	3 cut(s)	155, 243, 1608
BsaWI	WCCGGW	2 cut(s)	108, 1525
Bsc4I	CCNNNNNNNGG	2 cut(s)	400, 1690
Bse8I	GATNNNNATC	1 cut(s)	43
BseAI	TCCGGA	1 cut(s)	1525
BseBI	CCWGG	1 cut(s)	156
BseCI	ATCGAT	2 cut(s)	41, 471
BseDI	CCNNGG	3 cut(s)	155, 243, 1608
BseGI	GGATG	6 cut(s)	190, 784, 1303, 1523, 1534, 1566
BseJI	GATNNNNATC	1 cut(s)	43
BseLI	CCNNNNNNNGG	2 cut(s)	400, 1690
BseMII	CTCAG	4 cut(s)	80, 251, 480, 1786
BseSI	GKGCMC	2 cut(s)	463, 706
BseXI	GCAGC	5 cut(s)	1181, 1312, 1347, 1363, 1786
BseYI	CCCAGC	1 cut(s)	938
Bsh1236I	CGCG	1 cut(s)	287
Bsh1285I	CGRYCG	1 cut(s)	41
BshFI	GGCC	6 cut(s)	5, 226, 242, 311, 561, 1683
BshVI	ATCGAT	2 cut(s)	41, 471
BsiEI	CGRYCG	1 cut(s)	41
BsiHKAI	GWGCWC	5 cut(s)	463, 648, 706, 1011, 1217
BsiSI	CCGG	3 cut(s)	109, 683, 1526
BslI	CCNNNNNNNGG	2 cut(s)	400, 1690
BsmAI	GTCTC	2 cut(s)	64, 1775
BsmI	GAATGC	1 cut(s)	1720
BsnI	GGCC	6 cut(s)	5, 226, 242, 311, 561, 1683
Bsp119I	TTCGAA	1 cut(s)	454
Bsp1286I	GDGCHC	5 cut(s)	463, 648, 706, 1011, 1217
Bsp13I	TCCGGA	1 cut(s)	1525
Bsp143I	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
Bsp19I	CCATGG	1 cut(s)	1608
BspACI	CCGC	3 cut(s)	287, 890, 1366
BspANI	GGCC	6 cut(s)	5, 226, 242, 311, 561, 1683
BspCNI	CTCAG	4 cut(s)	79, 252, 481, 1785
BspDI	ATCGAT	2 cut(s)	41, 471
BspEI	TCCGGA	1 cut(s)	1525
BspFNI	CGCG	1 cut(s)	287
BspHI	TCATGA	2 cut(s)	628, 1011
BspLI	GGNNCC	3 cut(s)	165, 225, 1310
BspMI	ACCTGC	1 cut(s)	1667
BspPI	GGATC	5 cut(s)	276, 385, 467, 1497, 1924
BspT104I	TTCGAA	1 cut(s)	454
BspTI	CTTAAG	1 cut(s)	1909
BssECI	CCNNGG	3 cut(s)	155, 243, 1608
BssMI	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
BssNI	GRCGYC	1 cut(s)	1471
BssSI	CACGAG	1 cut(s)	1499
BssT1I	CCWWGG	2 cut(s)	243, 1608
Bst2BI	CACGAG	1 cut(s)	1499
Bst2UI	CCWGG	1 cut(s)	156
Bst4CI	ACNGT	6 cut(s)	677, 691, 701, 898, 1349, 1412
Bst6I	CTCTTC	3 cut(s)	341, 1284, 1788
BstACI	GRCGYC	1 cut(s)	1471
BstAFI	CTTAAG	1 cut(s)	1909
BstBAI	YACGTR	1 cut(s)	464
BstBI	TTCGAA	1 cut(s)	454
BstC8I	GCNNGC	3 cut(s)	572, 1783, 1787
BstDEI	CTNAG	5 cut(s)	66, 260, 489, 516, 1772
BstDSI	CCRYGG	1 cut(s)	1608
BstF5I	GGATG	6 cut(s)	190, 784, 1303, 1523, 1534, 1566
BstFNI	CGCG	1 cut(s)	287
BstKTI	GATC	9 cut(s)	41, 271, 393, 475, 603, 1344, 1492, 1846, 1919
BstMAI	GTCTC	2 cut(s)	64, 1775
BstMBI	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
BstMCI	CGRYCG	1 cut(s)	41
BstMWI	GCNNNNNNNGC	3 cut(s)	239, 567, 1166
BstNI	CCWGG	1 cut(s)	156
BstNSI	RCATGY	2 cut(s)	574, 979
BstSCI	CCNGG	2 cut(s)	154, 681
BstSLI	GKGCMC	2 cut(s)	463, 706
BstUI	CGCG	1 cut(s)	287
BstV1I	GCAGC	5 cut(s)	1181, 1312, 1347, 1363, 1786
BstV2I	GAAGAC	1 cut(s)	535
BstX2I	RGATCY	2 cut(s)	1489, 1916
BstXI	CCANNNNNNTGG	1 cut(s)	382
BstYI	RGATCY	2 cut(s)	1489, 1916
Bsu15I	ATCGAT	2 cut(s)	41, 471
BsuRI	GGCC	6 cut(s)	5, 226, 242, 311, 561, 1683
BsuTUI	ATCGAT	2 cut(s)	41, 471
BtgI	CCRYGG	1 cut(s)	1608
BtrI	CACGTC	1 cut(s)	1418
BtsCI	GGATG	6 cut(s)	190, 784, 1303, 1523, 1534, 1566
BtsIMutI	CAGTG	2 cut(s)	706, 909
BveI	ACCTGC	1 cut(s)	1667
Cac8I	GCNNGC	3 cut(s)	572, 1783, 1787
CciI	TCATGA	2 cut(s)	628, 1011
Cfr13I	GGNCC	4 cut(s)	163, 224, 637, 1682
ClaI	ATCGAT	2 cut(s)	41, 471
CseI	GACGC	1 cut(s)	1460
DdeI	CTNAG	5 cut(s)	66, 260, 489, 516, 1772
DpnI	GATC	9 cut(s)	40, 270, 392, 474, 602, 1343, 1491, 1845, 1918
DpnII	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
DraI	TTTAAA	1 cut(s)	1659
DrdI	GACNNNNNNGTC	1 cut(s)	170
DseDI	GACNNNNNNGTC	1 cut(s)	170
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	341, 1284, 1788
EarI	CTCTTC	3 cut(s)	341, 1284, 1788
EciI	GGCGGA	1 cut(s)	879
Ecl136II	GAGCTC	2 cut(s)	646, 1215
Eco130I	CCWWGG	2 cut(s)	243, 1608
Eco147I	AGGCCT	1 cut(s)	242
Eco24I	GRGCYC	2 cut(s)	648, 1217
Eco32I	GATATC	1 cut(s)	45
Eco47I	GGWCC	2 cut(s)	163, 637
Eco53kI	GAGCTC	2 cut(s)	646, 1215
Eco57I	CTGAAG	3 cut(s)	153, 275, 1001
Eco72I	CACGTG	1 cut(s)	464
EcoICRI	GAGCTC	2 cut(s)	646, 1215
EcoO109I	RGGNCCY	2 cut(s)	163, 224
EcoRII	CCWGG	1 cut(s)	154
EcoRV	GATATC	1 cut(s)	45
EcoT14I	CCWWGG	2 cut(s)	243, 1608
EcoT22I	ATGCAT	1 cut(s)	124
EcoT38I	GRGCYC	2 cut(s)	648, 1217
ErhI	CCWWGG	2 cut(s)	243, 1608
FauNDI	CATATG	1 cut(s)	1899
FbaI	TGATCA	1 cut(s)	1341
Fnu4HI	GCNGC	5 cut(s)	1170, 1326, 1336, 1377, 1800
FokI	GGATG	6 cut(s)	197, 771, 1310, 1530, 1541, 1573
FriOI	GRGCYC	2 cut(s)	648, 1217
Fsp4HI	GCNGC	5 cut(s)	1170, 1326, 1336, 1377, 1800
FspBI	CTAG	4 cut(s)	860, 929, 1428, 1671
GluI	GCNGC	5 cut(s)	1170, 1326, 1336, 1377, 1800
GsaI	CCCAGC	1 cut(s)	942
GsuI	CTGGAG	1 cut(s)	1140
HaeIII	GGCC	6 cut(s)	5, 226, 242, 311, 561, 1683
HapII	CCGG	3 cut(s)	109, 683, 1526
HgaI	GACGC	1 cut(s)	1460
Hin1I	GRCGYC	1 cut(s)	1471
HindIII	AAGCTT	3 cut(s)	849, 1134, 1272
HinfI	GANTC	5 cut(s)	501, 740, 1145, 1231, 1729
HpaII	CCGG	3 cut(s)	109, 683, 1526
HphI	GGTGA	3 cut(s)	434, 1123, 1843
Hpy166II	GTNNAC	6 cut(s)	195, 461, 526, 704, 782, 1415
Hpy188I	TCNGA	6 cut(s)	69, 127, 490, 1212, 1236, 1460
Hpy8I	GTNNAC	6 cut(s)	195, 461, 526, 704, 782, 1415
HpyAV	CCTTC	8 cut(s)	154, 177, 345, 504, 614, 763, 976, 1037
HpyCH4III	ACNGT	6 cut(s)	677, 691, 701, 898, 1349, 1412
HpyCH4IV	ACGT	2 cut(s)	463, 1417
HpyF10VI	GCNNNNNNNGC	3 cut(s)	239, 567, 1166
HpyF3I	CTNAG	5 cut(s)	66, 260, 489, 516, 1772
HpySE526I	ACGT	2 cut(s)	463, 1417
Hsp92I	GRCGYC	1 cut(s)	1471
Kpn2I	TCCGGA	1 cut(s)	1525
Ksp22I	TGATCA	1 cut(s)	1341
Kzo9I	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
LmnI	GCTCC	1 cut(s)	1159
Lsp1109I	GCAGC	5 cut(s)	1181, 1312, 1347, 1363, 1786
LweI	GCATC	6 cut(s)	131, 220, 603, 943, 1508, 1729
MaeI	CTAG	4 cut(s)	860, 929, 1428, 1671
MaeII	ACGT	2 cut(s)	463, 1417
MaeIII	GTNAC	4 cut(s)	587, 671, 691, 1111
MalI	GATC	9 cut(s)	40, 270, 392, 474, 602, 1343, 1491, 1845, 1918
MboI	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
MboII	GAAGA	8 cut(s)	358, 535, 962, 1198, 1301, 1519, 1625, 1805
MflI	RGATCY	2 cut(s)	1489, 1916
MhlI	GDGCHC	5 cut(s)	463, 648, 706, 1011, 1217
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MmeI	TCCRAC	2 cut(s)	385, 812
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	124
MroI	TCCGGA	1 cut(s)	1525
MroXI	GAANNNNTTC	1 cut(s)	212
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	1 cut(s)	380
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	479, 1325
MspCI	CTTAAG	1 cut(s)	1909
MspI	CCGG	3 cut(s)	109, 683, 1526
MspR9I	CCNGG	2 cut(s)	156, 683
Mva1269I	GAATGC	1 cut(s)	1720
MvaI	CCWGG	1 cut(s)	156
MvnI	CGCG	1 cut(s)	287
MwoI	GCNNNNNNNGC	3 cut(s)	239, 567, 1166
NciI	CCSGG	1 cut(s)	683
NcoI	CCATGG	1 cut(s)	1608
NdeI	CATATG	1 cut(s)	1899
NdeII	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
NlaIV	GGNNCC	3 cut(s)	165, 225, 1310
NmuCI	GTSAC	2 cut(s)	691, 1111
NsiI	ATGCAT	1 cut(s)	124
NspI	RCATGY	2 cut(s)	574, 979
NspV	TTCGAA	1 cut(s)	454
PaeI	GCATGC	1 cut(s)	574
PagI	TCATGA	2 cut(s)	628, 1011
PaqCI	CACCTGC	1 cut(s)	1667
PceI	AGGCCT	1 cut(s)	242
PcsI	WCGNNNNNNNCGW	1 cut(s)	460
PctI	GAATGC	1 cut(s)	1720
PdmI	GAANNNNTTC	1 cut(s)	212
PfeI	GAWTC	5 cut(s)	501, 740, 1145, 1231, 1729
PflFI	GACNNNGTC	1 cut(s)	689
PfoI	TCCNGGA	1 cut(s)	681
PkrI	GCNGC	5 cut(s)	1171, 1327, 1337, 1378, 1801
Ple19I	CGATCG	1 cut(s)	41
PmaCI	CACGTG	1 cut(s)	464
PmlI	CACGTG	1 cut(s)	464
Ppu21I	YACGTR	1 cut(s)	464
PpuMI	RGGWCCY	1 cut(s)	163
PshBI	ATTAAT	1 cut(s)	1065
PsiI	TTATAA	4 cut(s)	497, 1119, 1542, 1752
Psp124BI	GAGCTC	2 cut(s)	648, 1217
Psp5II	RGGWCCY	1 cut(s)	163
Psp6I	CCWGG	1 cut(s)	154
PspCI	CACGTG	1 cut(s)	464
PspFI	CCCAGC	1 cut(s)	938
PspGI	CCWGG	1 cut(s)	154
PspN4I	GGNNCC	3 cut(s)	165, 225, 1310
PspPI	GGNCC	4 cut(s)	163, 224, 637, 1682
PspPPI	RGGWCCY	1 cut(s)	163
PsuI	RGATCY	2 cut(s)	1489, 1916
PsyI	GACNNNGTC	1 cut(s)	689
PvuI	CGATCG	1 cut(s)	41
PvuII	CAGCTG	2 cut(s)	479, 1325
RseI	CAYNNNNRTG	1 cut(s)	380
SacI	GAGCTC	2 cut(s)	648, 1217
SatI	GCNGC	5 cut(s)	1170, 1326, 1336, 1377, 1800
Sau3AI	GATC	9 cut(s)	38, 268, 390, 472, 600, 1341, 1489, 1843, 1916
Sau96I	GGNCC	4 cut(s)	163, 224, 637, 1682
ScrFI	CCNGG	2 cut(s)	156, 683
SduI	GDGCHC	5 cut(s)	463, 648, 706, 1011, 1217
SfaNI	GCATC	6 cut(s)	131, 220, 603, 943, 1508, 1729
SfuI	TTCGAA	1 cut(s)	454
SinI	GGWCC	2 cut(s)	163, 637
SmiMI	CAYNNNNRTG	1 cut(s)	380
SmlI	CTYRAG	3 cut(s)	293, 1868, 1909
SmoI	CTYRAG	3 cut(s)	293, 1868, 1909
SphI	GCATGC	1 cut(s)	574
SseBI	AGGCCT	1 cut(s)	242
SsiI	CCGC	3 cut(s)	287, 890, 1366
SspMI	CTAG	4 cut(s)	860, 929, 1428, 1671
SstI	GAGCTC	2 cut(s)	648, 1217
StuI	AGGCCT	1 cut(s)	242
StyD4I	CCNGG	2 cut(s)	154, 681
StyI	CCWWGG	2 cut(s)	243, 1608
TaaI	ACNGT	6 cut(s)	677, 691, 701, 898, 1349, 1412
TaiI	ACGT	2 cut(s)	466, 1420
TaqI	TCGA	7 cut(s)	37, 41, 306, 454, 471, 1229, 1777
TfiI	GAWTC	5 cut(s)	501, 740, 1145, 1231, 1729
TscAI	CASTG	2 cut(s)	706, 916
TseFI	GTSAC	2 cut(s)	691, 1111
TseI	GCWGC	5 cut(s)	1169, 1325, 1335, 1376, 1799
Tsp45I	GTSAC	2 cut(s)	691, 1111
TspGWI	ACGGA	1 cut(s)	383
TspRI	CASTG	2 cut(s)	706, 916
Tth111I	GACNNNGTC	1 cut(s)	689
Vha464I	CTTAAG	1 cut(s)	1909
VneI	GTGCAC	2 cut(s)	459, 702
VpaK11BI	GGWCC	2 cut(s)	163, 637
VspI	ATTAAT	1 cut(s)	1065
XapI	RAATTY	2 cut(s)	210, 355
XceI	RCATGY	2 cut(s)	574, 979
XcmI	CCANNNNNNNNNTGG	1 cut(s)	445
XmnI	GAANNNNTTC	1 cut(s)	212
XspI	CTAG	4 cut(s)	860, 929, 1428, 1671
Zsp2I	ATGCAT	1 cut(s)	124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.