Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0006499

Physical Location & Seq

Genomic Coordinates Reverse (-)

50861810 .. 50867035

Gene Structure 5226 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0398200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

ATGGAAGAAGAAAACCAAAACGGGGAGGCGCCCCAAAAATTCCCAGCATTTCCTTACAAACCCTACGCCATCCAGATCGACTTCATGAACGCCCTTTATCACTCTCTCAACCAAGGCGGCCTCTCCATGCTCGAAAGCCCCACCGGGACTGGTAAGACTCTAAGTATCATTTGTAGTGCTTTGCAATGGGTGGTTGATAAGAGGGAGCAAGAGAAGTTGAAAAAGGAGGTTGAAACTGACAATAGTAGTGCTAAAGGTGGTGAATTGGGCTCGGATGATGAACCTGATTGGATGAGGAATGCTGTTATTAACAAAGACATTCAGGCTGAGGAGAAGAAGTCTAGGAAGAAGGATAAATTTGGGAGTGGATTTCGCAGGGTTGATAAGAGAAAGAACCATGCAAATTGTACTGATTTGTTCTCTCGGCGTGTTGAGGAAGAGTCTTGCACAAAGAAGGAGGGAAAGAGTTTGCAAGTGAGCAGTGATGCTGTGGATTTGAATGATGAGGAGGAGTTTTTGCTGGAAGAACATGAAAGTGATGAGGAAGGTGTTCCGTGTGCTGGGAAGTTGAAGAGGAAGGTTGCTGGGGTTTCTCTTAGTTTGTCAAGTGACGAGGAAGATGGAGCAGATGGGTCTAGTGATGATGAAGATGAGGGGGAAGAGAAGTTGAAGGTTTATTTTTGTAGTCGGACACATTCACAGCTTTCACAGTTCATAAAGGAGTTGAGAAAGACAGTCTTTGCTAATGAGATGAAGGTGGTTTGTTTGGGCTCAAGGAAGAATTTCTGCATTAACCAAGAGGTATTGAAACTTGGAAACTCAACTCCCATCAATGAACGCTGTTTGGAACTTCAGAAAAACAAGAATAAGGAAGTTTCTAAAATCAAGAGCATAGGTGCAGGAGGAAGGATTCGCAGGAACAAGGCTTCTTGTGGATGCCCAATGCTTAGGAAACATAAACAACAGAAAGAATTCAAGAGCATGATGTCTCAACTCGGTGCCTTGGATATTGAAGATCTTGTTCATCAGGGAAGGAACATGAGGTGTTGTCCATATTATGGCTCCCGAAGCATGGTTCCTGCTGCTGATCTGGTGGTTCTACCATATCAATCTCTTCTTTCAAAAGCATCCCGCGAATCACTGGGTCTGAATCTAAAGAACAATATTATTATCATAGACGAGGCCCACAATCTAGCTGACTCCTTAATCAGCATGTATGACTCAAAAATCACTTTGTCACAGCTGGAAGATGTGCAGTGCAATATTGAAAAGTACTTTGCAAGGTTTAGCAATCTCTTGGGACCTGGAAACCGCAGATACATCCAAACGCTCACAGTGCTCACTCGGGCTTTTCTTCGAGTTTTGCTCAATGAGGACACCGGTTATGAAGGTTCTTGCCATGATACTGAAAACTCTTCTGGAGAAAATGGCATGCCTACTTCCTCTATGGCCATCAATGATTTTTTATTTTCACTCAATATAGACAACATCAACTTGGTTAAATTGCTTGAGTATATAAAGGAAAGCAAAATCATGCACAAGATCAGTGGATATGGGGATAAACTAGCCAGCTTTGAAAAAAGCTTGCCACTTGATGGTAGTGAAGAAAGAAGCACTCTGTCCAGTTTCCAGGCATTAGCTGATTTTTTGCTATCACTGACAAACAAAGATGGTGATGGGAGGATTATCATTTCACGGACTAAACCAACATGCTCTGGGCAGCAAGGGGGATACATCAAATATGTTATGCTCACAGGGGAGAAGATATTTTCTGAGATTGTGGAACAGGCACATGCTGTGCTACTGGCTGGGGGAACTCTGCAACCCATAGAAGAAACGAGGGAGCGGCTTTTTCCTTGGTTACCACCGAACCAGTTGCATTTCTTTTCATGTAGCCACATTGTTCCTCCAGAGAGCATTTTGCCAGTTGCAGTTGCTCGTGGCCCTTCTGGTCAGCCGTTCGATTTTAGTTACAGCTCTAGAAGGTCATCAATCATGATACAGGAGCTAGGGCGTTTGGTTTGCAATTTGGTGACAGTGGTTCCTGAAGGAATCGTTGTCTTCTTCTCTTCATTTGATTATGAAGGAGAGGTGTACAATGCATGGGAGGCTTCAGGCATCCTCGATAGAATTAAGAAGAAGAAGCGTCTATTTAGAGAGCCTAGGAAGAATACATATGTAGAATCTGTTCTGAAGGAGTACAAGGAAACAATTGATACCTTGTCTTCAGGAGAAAGGAAAGAAAATCCTTCTCAGAGTGGTGCCATACTTCTTGCTGTTGTTGGTGGTAAGATATCGGAGGGCATAAACTTGAGTGATGGGATGGGTCGGTGTATAGTCATGGTTGGACTGCCCTATGCGAGCCCTTCTGATGTTGAGCTGATGGAGAGGGTGAAGCACATTGAAGGATTGGGAAATTCAAATTCGATTAAGATGACCAATTCTTTACGTGGTAATGAACTTTACAGTGGAGAAGCACATGAAGGATTTGATATCCTCCGAAGTTGCAGACACAGAGGAAAAGAATATTATGAGAATCTTTGCATGAAAGCTGTAAACCAGTCCATCGGTAGAGCAATCCGGCATATAAATGATTATGCAGCAATTTTACTAGTTGATACACGCTATGCAGCTGATTCCTCAACACGGAATTTCAGGCATCCAACTAGCAAGCTCCCACAGTGGATAAAGGATCGTTTCGTTGCTTCAGGCTTCTTAACGCAGAGCACTGCTTTTGTTTGTTCTTGGCTTCCTAGTGCTGAGCCCTATCTTGAAGCTGAAGTCACATCCTTGTACATTTTTATTTCAATGTTGTTTGTTCTTGGCTTCTTAGTGCTGAGCCCTATCTTGAAGCTGAAGTCACAACGTATCCGACTAATGGGAAAGGTATGCGGCCAAATAGAACTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005794 GO:0005811 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0031090 GO:0031410 GO:0031901 GO:0031902 GO:0031982 GO:0031984 GO:0034593 GO:0034595 GO:0036092 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043813 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0052866 GO:0071704 GO:0090407 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0106018 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Syja_N

Protein Analysis

969

Amino Acids

108.52

Weight (kDa)

6.45

Isoelectric Point (pI)

49.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	227 - 415	1e-52	DEAD_2
Helicase_C_2	PF13307	675 - 886	7.2e-42	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14205 AT3G14205
fragaria_vesca	FvH4_6g35480 FvH4_6g35480 FvH4_6g35480
malus_domestica	MD09G1175500.v1.1 MD17G1151300.v1.1
prunus_persica	Prupe.3G034500_v2.0.a1
pyrus_communis	pycom09g09320 pycom17g14460
rosa_chinensis	RchiOBHm_Chr2g0147031
rosa_laevigata	RLG00000020240
rosa_multiflora	Rmu_co8447827.1_g000001 Rmu_sc0001880.1_g000033 Rmu_sc0036715.1_g000001
rosa_roxburghii	Rroxscaffold_2G00099750
rosa_rugosa	Rorug02G0398100 Rorug02G0398100 Rorug02G0398200
rosa_samantha	Rh2AG455200 Rh2BG467600 Rh2CG442600 Rh2DG476800 Rh2DG476900
rosa_wichuraiana	Rw2G037140 Rw2G037240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	28, 998, 2261
AccB7I	CCANNNNNTGG	2 cut(s)	1058, 1595
AccBSI	CCGCTC	1 cut(s)	1846
AccII	CGCG	1 cut(s)	1134
AciI	CCGC	5 cut(s)	117, 1132, 1312, 1846, 2892
AclWI	GGATC	1 cut(s)	2700
AcoI	YGGCCR	2 cut(s)	1449, 2893
AcsI	RAATTY	7 cut(s)	38, 356, 781, 971, 2416, 2422, 2650
AcuI	CTGAAG	8 cut(s)	836, 2067, 2097, 2211, 2213, 2691, 2799, 2876
AcyI	GRCGYC	1 cut(s)	29
AfaI	GTAC	5 cut(s)	409, 1274, 2096, 2201, 2795
AfiI	CCNNNNNNNGG	7 cut(s)	22, 560, 1058, 1072, 1595, 2646, 2878
AgeI	ACCGGT	1 cut(s)	1379
AhlI	ACTAGT	1 cut(s)	2611
AjnI	CCWGG	2 cut(s)	1303, 1629
Alw21I	GWGCWC	2 cut(s)	1341, 2729
Alw26I	GTCTC	1 cut(s)	993
AlwI	GGATC	1 cut(s)	2700
Ama87I	CYCGRG	1 cut(s)	1344
AoxI	GGCC	5 cut(s)	118, 1182, 1449, 1942, 2893
ApeKI	GCWGC	4 cut(s)	1082, 1720, 2600, 2630
ApoI	RAATTY	7 cut(s)	38, 356, 781, 971, 2416, 2422, 2650
AsiGI	ACCGGT	1 cut(s)	1379
Asp700I	GAANNNNTTC	4 cut(s)	549, 782, 971, 2187
AspA2I	CCTAGG	1 cut(s)	2162
AspLEI	GCGC	1 cut(s)	31
AspS9I	GGNCC	3 cut(s)	1183, 1301, 1943
AsuC2I	CCSGG	1 cut(s)	145
AsuHPI	GGTGA	4 cut(s)	272, 1685, 2044, 2404
AvaI	CYCGRG	1 cut(s)	1344
AvaII	GGWCC	1 cut(s)	1301
AvrII	CCTAGG	1 cut(s)	2162
BalI	TGGCCA	1 cut(s)	1451
BanI	GGYRCC	3 cut(s)	28, 998, 2261
BanII	GRGCYC	5 cut(s)	272, 773, 2366, 2766, 2843
BauI	CACGAG	1 cut(s)	1938
BbsI	GAAGAC	2 cut(s)	2053, 2217
Bbv12I	GWGCWC	2 cut(s)	1341, 2729
BbvCI	CCTCAGC	1 cut(s)	327
BbvI	GCAGC	4 cut(s)	1069, 1732, 2612, 2642
BceAI	ACGGC	1 cut(s)	1942
BcgI	CGANNNNNNTGC	2 cut(s)	1858, 1892
BciT130I	CCWGG	2 cut(s)	1305, 1631
BciVI	GTATCC	2 cut(s)	1724, 2879
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	1 cut(s)	993
BcuI	ACTAGT	1 cut(s)	2611
BfoI	RGCGCY	1 cut(s)	32
BfuI	GTATCC	2 cut(s)	1724, 2879
BglII	AGATCT	1 cut(s)	1015
BisI	GCNGC	7 cut(s)	118, 1083, 1721, 1847, 2601, 2631, 2893
BlnI	CCTAGG	1 cut(s)	2162
BlpI	GCTNAGC	2 cut(s)	2760, 2837
BlsI	GCNGC	7 cut(s)	119, 1084, 1722, 1848, 2602, 2632, 2894
BmcAI	AGTACT	1 cut(s)	1274
Bme1390I	CCNGG	3 cut(s)	145, 1305, 1631
Bme18I	GGWCC	1 cut(s)	1301
BmeT110I	CYCGRG	1 cut(s)	1344
BmgT120I	GGNCC	3 cut(s)	1183, 1301, 1943
BmiI	GGNNCC	7 cut(s)	30, 1000, 1063, 1077, 1302, 2043, 2263
BmrFI	CCNGG	3 cut(s)	145, 1305, 1631
BmrI	ACTGGG	1 cut(s)	1151
BmsI	GCATC	5 cut(s)	475, 926, 1136, 2127, 2668
BmuI	ACTGGG	1 cut(s)	1151
BpiI	GAAGAC	2 cut(s)	2053, 2217
BplI	GAGNNNNNCTC	2 cut(s)	1350, 1382
BpmI	CTGGAG	2 cut(s)	1440, 1893
Bpu10I	CCTNAGC	2 cut(s)	327, 947
Bpu1102I	GCTNAGC	2 cut(s)	2760, 2837
BpuEI	CTTGAG	3 cut(s)	757, 1529, 2332
BpuMI	CCSGG	1 cut(s)	145
BsaAI	YACGTR	1 cut(s)	2450
BsaHI	GRCGYC	1 cut(s)	29
BsaJI	CCNNGG	4 cut(s)	112, 1002, 1856, 2162
BsaWI	WCCGGW	1 cut(s)	1379
BsaXI	ACNNNNNCTCC	2 cut(s)	323, 353
Bsc4I	CCNNNNNNNGG	7 cut(s)	22, 560, 1058, 1072, 1595, 2646, 2878
Bse118I	RCCGGY	1 cut(s)	1379
Bse1I	ACTGG	7 cut(s)	154, 1146, 1623, 1809, 1873, 1925, 2560
Bse3DI	GCAATG	1 cut(s)	191
BseBI	CCWGG	2 cut(s)	1305, 1631
BseDI	CCNNGG	4 cut(s)	112, 1002, 1856, 2162
BseLI	CCNNNNNNNGG	7 cut(s)	22, 560, 1058, 1072, 1595, 2646, 2878
BseMI	GCAATG	1 cut(s)	191
BseMII	CTCAG	5 cut(s)	318, 1764, 2267, 2751, 2828
BseNI	ACTGG	7 cut(s)	154, 1146, 1623, 1809, 1873, 1925, 2560
BseRI	GAGGAG	3 cut(s)	344, 521, 524
BseXI	GCAGC	4 cut(s)	1069, 1732, 2612, 2642
BseYI	CCCAGC	4 cut(s)	43, 560, 584, 1808
BsgI	GTGCAG	2 cut(s)	918, 1274
Bsh1236I	CGCG	1 cut(s)	1134
BshFI	GGCC	5 cut(s)	120, 1184, 1451, 1944, 2895
BshNI	GGYRCC	3 cut(s)	28, 998, 2261
BshTI	ACCGGT	1 cut(s)	1379
BsiHKAI	GWGCWC	2 cut(s)	1341, 2729
BsiHKCI	CYCGRG	1 cut(s)	1344
BsiSI	CCGG	3 cut(s)	144, 1380, 2581
BslFI	GGGAC	2 cut(s)	160, 1314
BslI	CCNNNNNNNGG	7 cut(s)	22, 560, 1058, 1072, 1595, 2646, 2878
BsmAI	GTCTC	1 cut(s)	993
BsmFI	GGGAC	2 cut(s)	160, 1314
BsmI	GAATGC	1 cut(s)	304
BsnI	GGCC	5 cut(s)	120, 1184, 1451, 1944, 2895
BsoBI	CYCGRG	1 cut(s)	1344
Bsp1286I	GDGCHC	7 cut(s)	272, 773, 1341, 2366, 2729, 2766, 2843
Bsp1407I	TGTACA	2 cut(s)	2094, 2793
Bsp143I	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
Bsp1720I	GCTNAGC	2 cut(s)	2760, 2837
BspACI	CCGC	5 cut(s)	117, 1132, 1312, 1846, 2892
BspANI	GGCC	5 cut(s)	120, 1184, 1451, 1944, 2895
BspCNI	CTCAG	5 cut(s)	319, 1765, 2266, 2752, 2829
BspFNI	CGCG	1 cut(s)	1134
BspHI	TCATGA	2 cut(s)	84, 1995
BspLI	GGNNCC	7 cut(s)	30, 1000, 1063, 1077, 1302, 2043, 2263
BspPI	GGATC	1 cut(s)	2700
BspT107I	GGYRCC	3 cut(s)	28, 998, 2261
BsrBI	CCGCTC	1 cut(s)	1846
BsrDI	GCAATG	1 cut(s)	191
BsrFI	RCCGGY	1 cut(s)	1379
BsrGI	TGTACA	2 cut(s)	2094, 2793
BsrI	ACTGG	7 cut(s)	154, 1146, 1623, 1809, 1873, 1925, 2560
BssAI	RCCGGY	1 cut(s)	1379
BssECI	CCNNGG	4 cut(s)	112, 1002, 1856, 2162
BssMI	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
BssNI	GRCGYC	1 cut(s)	29
BssSI	CACGAG	1 cut(s)	1938
BssT1I	CCWWGG	4 cut(s)	112, 1002, 1856, 2162
Bst2BI	CACGAG	1 cut(s)	1938
Bst2UI	CCWGG	2 cut(s)	1305, 1631
Bst4CI	ACNGT	6 cut(s)	711, 736, 1336, 2038, 2468, 2682
Bst6I	CTCTTC	6 cut(s)	432, 566, 654, 1119, 1420, 2074
BstACI	GRCGYC	1 cut(s)	29
BstAUI	TGTACA	2 cut(s)	2094, 2793
BstBAI	YACGTR	1 cut(s)	2450
BstC8I	GCNNGC	5 cut(s)	1433, 1570, 1586, 2362, 2672
BstDEI	CTNAG	9 cut(s)	161, 327, 596, 947, 1773, 2253, 2760, 2830, 2837
BstEII	GGTNACC	1 cut(s)	1860
BstFNI	CGCG	1 cut(s)	1134
BstH2I	RGCGCY	1 cut(s)	32
BstHHI	GCGC	1 cut(s)	31
BstKTI	GATC	5 cut(s)	78, 1018, 1090, 1545, 2695
BstMAI	GTCTC	1 cut(s)	993
BstMBI	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
BstMWI	GCNNNNNNNGC	3 cut(s)	1068, 1336, 2108
BstNI	CCWGG	2 cut(s)	1305, 1631
BstNSI	RCATGY	4 cut(s)	1216, 1435, 1713, 1796
BstPI	GGTNACC	1 cut(s)	1860
BstSCI	CCNGG	3 cut(s)	143, 1303, 1629
BstUI	CGCG	1 cut(s)	1134
BstV1I	GCAGC	4 cut(s)	1069, 1732, 2612, 2642
BstV2I	GAAGAC	2 cut(s)	2053, 2217
BstX2I	RGATCY	1 cut(s)	1015
BstYI	RGATCY	1 cut(s)	1015
BsuI	GTATCC	2 cut(s)	1724, 2879
BsuRI	GGCC	5 cut(s)	120, 1184, 1451, 1944, 2895
BtsI	GCAGTG	3 cut(s)	487, 1262, 2727
Cac8I	GCNNGC	5 cut(s)	1433, 1570, 1586, 2362, 2672
CciI	TCATGA	2 cut(s)	84, 1995
CfoI	GCGC	1 cut(s)	31
Cfr10I	RCCGGY	1 cut(s)	1379
Cfr13I	GGNCC	3 cut(s)	1183, 1301, 1943
CseI	GACGC	1 cut(s)	2135
Csp6I	GTAC	5 cut(s)	408, 1273, 2095, 2200, 2794
CspAI	ACCGGT	1 cut(s)	1379
CspCI	CAANNNNNGTGG	2 cut(s)	1528, 1563
CviQI	GTAC	5 cut(s)	408, 1273, 2095, 2200, 2794
DdeI	CTNAG	9 cut(s)	161, 327, 596, 947, 1773, 2253, 2760, 2830, 2837
DinI	GGCGCC	1 cut(s)	30
DpnI	GATC	5 cut(s)	77, 1017, 1089, 1544, 2694
DpnII	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
EaeI	YGGCCR	2 cut(s)	1449, 2893
Eam1104I	CTCTTC	6 cut(s)	432, 566, 654, 1119, 1420, 2074
EarI	CTCTTC	6 cut(s)	432, 566, 654, 1119, 1420, 2074
Eco130I	CCWWGG	4 cut(s)	112, 1002, 1856, 2162
Eco24I	GRGCYC	5 cut(s)	272, 773, 2366, 2766, 2843
Eco32I	GATATC	2 cut(s)	2295, 2494
Eco47I	GGWCC	1 cut(s)	1301
Eco57I	CTGAAG	8 cut(s)	836, 2067, 2097, 2211, 2213, 2691, 2799, 2876
Eco88I	CYCGRG	1 cut(s)	1344
Eco91I	GGTNACC	1 cut(s)	1860
EcoO109I	RGGNCCY	1 cut(s)	1301
EcoO65I	GGTNACC	1 cut(s)	1860
EcoRI	GAATTC	1 cut(s)	971
EcoRII	CCWGG	2 cut(s)	1303, 1629
EcoRV	GATATC	2 cut(s)	2295, 2494
EcoT14I	CCWWGG	4 cut(s)	112, 1002, 1856, 2162
EcoT22I	ATGCAT	1 cut(s)	2104
EcoT38I	GRGCYC	5 cut(s)	272, 773, 2366, 2766, 2843
EgeI	GGCGCC	1 cut(s)	30
EheI	GGCGCC	1 cut(s)	30
ErhI	CCWWGG	4 cut(s)	112, 1002, 1856, 2162
FalI	AAGNNNNNCTT	2 cut(s)	722, 754
FaqI	GGGAC	2 cut(s)	160, 1314
FauI	CCCGC	1 cut(s)	1139
FauNDI	CATATG	1 cut(s)	2176
Fnu4HI	GCNGC	7 cut(s)	118, 1083, 1721, 1847, 2601, 2631, 2893
FriOI	GRGCYC	5 cut(s)	272, 773, 2366, 2766, 2843
Fsp4HI	GCNGC	7 cut(s)	118, 1083, 1721, 1847, 2601, 2631, 2893
GlaI	GCGC	1 cut(s)	30
GluI	GCNGC	7 cut(s)	118, 1083, 1721, 1847, 2601, 2631, 2893
GsaI	CCCAGC	4 cut(s)	47, 564, 588, 1812
GsuI	CTGGAG	2 cut(s)	1440, 1893
HaeII	RGCGCY	1 cut(s)	32
HaeIII	GGCC	5 cut(s)	120, 1184, 1451, 1944, 2895
HapII	CCGG	3 cut(s)	144, 1380, 2581
HgaI	GACGC	1 cut(s)	2135
HhaI	GCGC	1 cut(s)	31
Hin1I	GRCGYC	1 cut(s)	29
Hin6I	GCGC	1 cut(s)	29
HinP1I	GCGC	1 cut(s)	29
HindIII	AAGCTT	1 cut(s)	1582
HpaII	CCGG	3 cut(s)	144, 1380, 2581
HphI	GGTGA	4 cut(s)	272, 1685, 2044, 2404
Hpy166II	GTNNAC	2 cut(s)	2095, 2557
Hpy8I	GTNNAC	2 cut(s)	2095, 2557
HpyCH4III	ACNGT	6 cut(s)	711, 736, 1336, 2038, 2468, 2682
HpyCH4IV	ACGT	2 cut(s)	2449, 2866
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1068, 1336, 2108
HpyF3I	CTNAG	9 cut(s)	161, 327, 596, 947, 1773, 2253, 2760, 2830, 2837
HpySE526I	ACGT	2 cut(s)	2449, 2866
Hsp92I	GRCGYC	1 cut(s)	29
HspAI	GCGC	1 cut(s)	29
KasI	GGCGCC	1 cut(s)	28
Kzo9I	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
LmnI	GCTCC	6 cut(s)	205, 623, 1067, 1843, 2005, 2679
Lsp1109I	GCAGC	4 cut(s)	1069, 1732, 2612, 2642
LweI	GCATC	5 cut(s)	475, 926, 1136, 2127, 2668
MaeII	ACGT	2 cut(s)	2449, 2866
MaeIII	GTNAC	7 cut(s)	608, 1236, 1860, 1970, 2032, 2782, 2859
MalI	GATC	5 cut(s)	77, 1017, 1089, 1544, 2694
MbiI	CCGCTC	1 cut(s)	1846
MboI	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
MfeI	CAATTG	1 cut(s)	2211
MflI	RGATCY	1 cut(s)	1015
MhlI	GDGCHC	7 cut(s)	272, 773, 1341, 2366, 2729, 2766, 2843
MlsI	TGGCCA	1 cut(s)	1451
MluNI	TGGCCA	1 cut(s)	1451
Mly113I	GGCGCC	1 cut(s)	29
MlyI	GAGTC	4 cut(s)	151, 449, 1193, 1214
MmeI	TCCRAC	4 cut(s)	668, 2326, 2687, 2896
Mox20I	TGGCCA	1 cut(s)	1451
Mph1103I	ATGCAT	1 cut(s)	2104
MroXI	GAANNNNTTC	4 cut(s)	549, 782, 971, 2187
MscI	TGGCCA	1 cut(s)	1451
MseI	TTAA	7 cut(s)	309, 792, 1205, 1500, 2133, 2430, 2717
MslI	CAYNNNNRTG	2 cut(s)	534, 2589
Msp20I	TGGCCA	1 cut(s)	1451
MspA1I	CMGCKG	2 cut(s)	1243, 2633
MspI	CCGG	3 cut(s)	144, 1380, 2581
MspR9I	CCNGG	3 cut(s)	145, 1305, 1631
MunI	CAATTG	1 cut(s)	2211
Mva1269I	GAATGC	1 cut(s)	304
MvaI	CCWGG	2 cut(s)	1305, 1631
MvnI	CGCG	1 cut(s)	1134
MwoI	GCNNNNNNNGC	3 cut(s)	1068, 1336, 2108
NarI	GGCGCC	1 cut(s)	29
NciI	CCSGG	1 cut(s)	145
NdeI	CATATG	1 cut(s)	2176
NdeII	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
NlaIV	GGNNCC	7 cut(s)	30, 1000, 1063, 1077, 1302, 2043, 2263
NmeAIII	GCCGAG	1 cut(s)	403
NmuCI	GTSAC	5 cut(s)	608, 1236, 2032, 2782, 2859
NsiI	ATGCAT	1 cut(s)	2104
NspI	RCATGY	4 cut(s)	1216, 1435, 1713, 1796
PaeI	GCATGC	1 cut(s)	1435
PagI	TCATGA	2 cut(s)	84, 1995
PctI	GAATGC	1 cut(s)	304
PdmI	GAANNNNTTC	4 cut(s)	549, 782, 971, 2187
PfeI	GAWTC	7 cut(s)	910, 1136, 1150, 2052, 2183, 2536, 2636
PflMI	CCANNNNNTGG	2 cut(s)	1058, 1595
PinAI	ACCGGT	1 cut(s)	1379
PkrI	GCNGC	7 cut(s)	119, 1084, 1722, 1848, 2602, 2632, 2894
PleI	GAGTC	4 cut(s)	151, 448, 1193, 1214
PluTI	GGCGCC	1 cut(s)	32
PpsI	GAGTC	4 cut(s)	151, 448, 1193, 1214
Ppu21I	YACGTR	1 cut(s)	2450
PpuMI	RGGWCCY	1 cut(s)	1301
Psp5II	RGGWCCY	1 cut(s)	1301
Psp6I	CCWGG	2 cut(s)	1303, 1629
PspEI	GGTNACC	1 cut(s)	1860
PspFI	CCCAGC	4 cut(s)	43, 560, 584, 1808
PspGI	CCWGG	2 cut(s)	1303, 1629
PspN4I	GGNNCC	7 cut(s)	30, 1000, 1063, 1077, 1302, 2043, 2263
PspPI	GGNCC	3 cut(s)	1183, 1301, 1943
PspPPI	RGGWCCY	1 cut(s)	1301
PsrI	GAACNNNNNNTAC	2 cut(s)	2172, 2204
PsuI	RGATCY	1 cut(s)	1015
PvuII	CAGCTG	2 cut(s)	1243, 2633
RsaI	GTAC	5 cut(s)	409, 1274, 2096, 2201, 2795
RsaNI	GTAC	5 cut(s)	408, 1273, 2095, 2200, 2794
RseI	CAYNNNNRTG	2 cut(s)	534, 2589
SaqAI	TTAA	7 cut(s)	309, 792, 1205, 1500, 2133, 2430, 2717
SatI	GCNGC	7 cut(s)	118, 1083, 1721, 1847, 2601, 2631, 2893
Sau3AI	GATC	5 cut(s)	75, 1015, 1087, 1542, 2692
Sau96I	GGNCC	3 cut(s)	1183, 1301, 1943
ScaI	AGTACT	1 cut(s)	1274
SchI	GAGTC	4 cut(s)	151, 449, 1193, 1214
ScrFI	CCNGG	3 cut(s)	145, 1305, 1631
SduI	GDGCHC	7 cut(s)	272, 773, 1341, 2366, 2729, 2766, 2843
SfaNI	GCATC	5 cut(s)	475, 926, 1136, 2127, 2668
SfoI	GGCGCC	1 cut(s)	30
SinI	GGWCC	1 cut(s)	1301
SmiMI	CAYNNNNRTG	2 cut(s)	534, 2589
SmlI	CTYRAG	3 cut(s)	772, 1508, 2311
SmoI	CTYRAG	3 cut(s)	772, 1508, 2311
SpeI	ACTAGT	1 cut(s)	2611
SphI	GCATGC	1 cut(s)	1435
SsiI	CCGC	5 cut(s)	117, 1132, 1312, 1846, 2892
SspDI	GGCGCC	1 cut(s)	28
SspI	AATATT	3 cut(s)	1165, 1264, 2528
StyD4I	CCNGG	3 cut(s)	143, 1303, 1629
StyI	CCWWGG	4 cut(s)	112, 1002, 1856, 2162
TaaI	ACNGT	6 cut(s)	711, 736, 1336, 2038, 2468, 2682
TaiI	ACGT	2 cut(s)	2452, 2869
TaqI	TCGA	6 cut(s)	78, 132, 1357, 1962, 2124, 2426
TatI	WGTACW	5 cut(s)	407, 1272, 2094, 2199, 2793
TauI	GCSGC	3 cut(s)	120, 1849, 2895
TfiI	GAWTC	7 cut(s)	910, 1136, 1150, 2052, 2183, 2536, 2636
Tru1I	TTAA	7 cut(s)	309, 792, 1205, 1500, 2133, 2430, 2717
Tru9I	TTAA	7 cut(s)	309, 792, 1205, 1500, 2133, 2430, 2717
TseFI	GTSAC	5 cut(s)	608, 1236, 2032, 2782, 2859
TseI	GCWGC	4 cut(s)	1082, 1720, 2600, 2630
Tsp45I	GTSAC	5 cut(s)	608, 1236, 2032, 2782, 2859
TspGWI	ACGGA	3 cut(s)	543, 1711, 2662
Van91I	CCANNNNNTGG	2 cut(s)	1058, 1595
VpaK11BI	GGWCC	1 cut(s)	1301
XapI	RAATTY	7 cut(s)	38, 356, 781, 971, 2416, 2422, 2650
XbaI	TCTAGA	1 cut(s)	1979
XceI	RCATGY	4 cut(s)	1216, 1435, 1713, 1796
XmaJI	CCTAGG	1 cut(s)	2162
XmnI	GAANNNNTTC	4 cut(s)	549, 782, 971, 2187
ZrmI	AGTACT	1 cut(s)	1274
Zsp2I	ATGCAT	1 cut(s)	2104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.