Rorug02G0594600

Aspartokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004938
Physical Location & Seq
Forward (+)
71642271 .. 71645023
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0594600.1

Sequence Viewer

Length: 2055 bp
ATGACCACCACCATGTCCTCCGCCACGGCCACCACCACCACCCTCCTCCTCCTCCTCCTCCTAACCCTAGTCGCCACCTCCACCTCCACCTCCACCTCAGCCCCAATTCTCGGCCTCGACACCTTCCTCACCCACCAGTCCCGCTACGACCGGCAAGCCTCCAACGACTCCTTCCAGTCTCTCTCCTCCACAATCCGCCACTCCCTCTCTCTCTCCTCGCCGTCTCTCTCCCACTCTCTCTCCTCCATCCTCTCCCTCACCATCCCTCTCTCTCTCCACGTCCGCCTCGTCGGCCCTTCCTTCCCTTCCTCCTCCGCCACTCTCCTCTCCTCCTTCCTCTCCGCCTCCCAGACCTCCGATCACTTCCACGTCATCACCCCCGCCGACGCCTCCGACTCTCACCGCTTGGCCGTCAAGCACTCCCTCCACCTCGACGTTAGTCACTCCCCCTCCCTCGCCTCTCAGCTCTCCAAAACCCTAGCCTCCGAGCTCGCCAATACCCCCGCCAGTCTCCGATCGCCGCTTGTCTCCGTCGCCTACGACTCAATCGACCGGATCATCAAGCAGGACTTCGAGAAAGAGAAGCCGGTCCACGGCGTTTACCTCTATTTCTTGGATTTGGGGCCCCAATCCAAGAGCTATGCTTACAGCTATGGATCTGGTGACTCCTCTCCTGGCTTCACCAAGTGCTTGGGCAGCGTTTGGACCGGGTCGGACCGCTACATTTGGATCGATTTGGGCGCCGGACCGGTCGACTACGGCCCGGCGCTTTCCGGCGACGGTGTCTTGCCGAAAGGCGAGTTTCATCCTCTGGCGGCGTTGCACGGCTCGCCGAAAGCTCAGAAGGCTTTGCTGGCGGACTTGGCTTCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTTGTTCCCTCTTTGAGAATTCCTGTTCAGTATGAGAATTCTTTGATTGTTCAGTTTATACATGTTTATGGAGGTGAGAGTGGTAAGGATTCGAGTGGTTTGGATTGGAAATCGATTGAGAGGACCTTTATGGATGAGGCTAAGGATAATGGCTTGTTGTTAGGGGATCAGTCTTTGAGTTTCAAGAATTATAAGGTGAAGTATTCGGAGTGCCCGATATGTTCATTTGCTATTGCCAGGTCGATTAATTCGTATACGTCGAGGTTCTTGTTTGATAACTATACGTTGATTGCGAGTGAGTATTTGGATTCGAAGAGATTGCATCAGATATTGTCTGACTCTGCTGAGGAGTTTAGGAGGGTTGCGGGGTTTCCTGAGGAAGATTTTGGCAGGGTGCTTCCTGTGTATGTGTTTGATTTGGACTACCACATGCTGTTGCTGCTCGATAGGTATCACCAAACTGTTGCTTTTAGAGATATGGTGATTGCAGTGAGGACCAGGAACACACAGACTGTGAGTGATTATAGCTGCAATGGCCGCCATGTGTTTACACAGACGAGGGAGCTTGAGAGACCACTTGTTGGTTCGATTTTACAGAGTATGTGGGGAGTGTCGCCGACTCATATGTTGTGGAGCCCTAGGCACAATACTACGTTGGTGGATTACACATGGAGTGTTGGGCAGACTCCATTTGGGCCGTTCTCGGAGATTTCATCACTATCATTCGTACAGAAGGACGCTGCTCGGAGAAATGTTCTTTTGACATCACTAAATTACAGCATATCGAGTGCTGTAGATGTTCTTGAATCCATAGCTGCACATGGTGGGGAGAGAAAGCTCCTGAAACGTAATCGACATGTTGAGTTCGTACAAAGGTGGAATTTCTTCAAGTACAAGCTGGACAAAGCAGTTTCTGCAATGTCACATTTGGACTTTGAGATGGCTTTATACTATATGAGGTCCTCAGATCATGATCTATATGCTATCCACTCTATTGTGTATCATGCCTCCCACGAATTGGAGGCATCCCTTGTATGTTTCAAGGATCCACCATTTCCGTGGTCTTTTGTGTTTATGGTTGCAGTGCTTCTCCTTCTTGGCTATTATATTAGTTCTAGAAAACACAAACTATTCAGAAGTAAGAGTAAGCAATTTTGA

Protein Analysis

684

Amino Acids

76.23

Weight (kDa)

7.17

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 89 - 302 3.7e-88 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AccB1I GGYRCC 1 cut(s) 740
AccB7I CCANNNNNTGG 2 cut(s) 1478, 1915
AccI GTMKAC 2 cut(s) 753, 1151
AclWI GGATC 6 cut(s) 563, 664, 737, 1071, 1937, 1950
AcoI YGGCCR 3 cut(s) 27, 408, 1432
AcsI RAATTY 3 cut(s) 915, 934, 1777
AcyI GRCGYC 2 cut(s) 387, 741
AdeI CACNNNGTG 2 cut(s) 687, 1721
AfaI GTAC 3 cut(s) 1626, 1767, 1790
AfiI CCNNNNNNNGG 5 cut(s) 110, 593, 876, 1478, 1915
AflIII ACRYGT 2 cut(s) 958, 1753
AgeI ACCGGT 1 cut(s) 748
AgsI TTSAA 4 cut(s) 1081, 1703, 1786, 1939
AjiI CACGTC 2 cut(s) 280, 370
AjnI CCWGG 4 cut(s) 673, 888, 1133, 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 5 cut(s) 183, 228, 515, 532, 1462
AlwI GGATC 6 cut(s) 563, 664, 737, 1071, 1937, 1950
AlwNI CAGNNNCTG 1 cut(s) 1811
AoxI GGCC 8 cut(s) 27, 112, 292, 408, 623, 760, 1432, 1592
ApaI GGGCCC 1 cut(s) 627
ApeKI GCWGC 5 cut(s) 696, 1336, 1425, 1637, 1712
ApoI RAATTY 3 cut(s) 915, 934, 1777
AseI ATTAAT 1 cut(s) 1143
AsiGI ACCGGT 1 cut(s) 748
Asp700I GAANNNNTTC 1 cut(s) 1781
AspA2I CCTAGG 1 cut(s) 1535
AspLEI GCGC 2 cut(s) 743, 769
AsuC2I CCSGG 2 cut(s) 709, 764
AsuII TTCGAA 1 cut(s) 1208
AvaII GGWCC 7 cut(s) 589, 705, 715, 746, 1020, 1392, 1857
AvrII CCTAGG 1 cut(s) 1535
AxyI CCTNAGG 1 cut(s) 1272
BaeGI GKGCMC 2 cut(s) 627, 1112
BamHI GGATCC 1 cut(s) 1942
BanI GGYRCC 1 cut(s) 740
BanII GRGCYC 3 cut(s) 492, 627, 1535
Bbv12I GWGCWC 1 cut(s) 492
BbvCI CCTCAGC 2 cut(s) 97, 1242
BbvI GCAGC 5 cut(s) 708, 1323, 1412, 1624, 1699
BccI CCATC 3 cut(s) 254, 269, 1831
BceAI ACGGC 7 cut(s) 42, 205, 395, 610, 775, 841, 1579
BcgI CGANNNNNNTGC 2 cut(s) 1198, 1232
BciT130I CCWGG 4 cut(s) 675, 890, 1135, 1396
BcnI CCSGG 2 cut(s) 709, 764
BcoDI GTCTC 5 cut(s) 183, 228, 515, 532, 1462
BfaI CTAG 4 cut(s) 68, 479, 1536, 2013
BfmI CTRYAG 1 cut(s) 1689
BfoI RGCGCY 2 cut(s) 744, 770
BglI GCCNNNNNGGC 1 cut(s) 291
BisI GCNGC 8 cut(s) 521, 697, 816, 1337, 1426, 1435, 1638, 1713
BlnI CCTAGG 1 cut(s) 1535
BlsI GCNGC 8 cut(s) 522, 698, 817, 1338, 1427, 1436, 1639, 1714
Bme1390I CCNGG 6 cut(s) 675, 709, 764, 890, 1135, 1396
Bme18I GGWCC 7 cut(s) 589, 705, 715, 746, 1020, 1392, 1857
BmgBI CACGTC 2 cut(s) 280, 370
BmiI GGNNCC 6 cut(s) 624, 625, 626, 742, 1532, 1944
BmrFI CCNGG 6 cut(s) 675, 709, 764, 890, 1135, 1396
BmsI GCATC 2 cut(s) 1228, 1931
BoxI GACNNNNGTC 1 cut(s) 438
Bpu10I CCTNAGC 3 cut(s) 97, 1038, 1242
Bpu14I TTCGAA 1 cut(s) 1208
BpuEI CTTGAG 1 cut(s) 1484
BpuMI CCSGG 2 cut(s) 709, 764
Bsa29I ATCGAT 2 cut(s) 732, 1010
BsaBI GATNNNNATC 2 cut(s) 1347, 1869
BsaHI GRCGYC 2 cut(s) 387, 741
BsaI GGTCTC 1 cut(s) 1462
BsaJI CCNNGG 5 cut(s) 24, 592, 870, 1535, 1955
BsaWI WCCGGW 2 cut(s) 552, 748
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 434, 464
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 593, 876, 1478, 1915
Bse118I RCCGGY 3 cut(s) 150, 586, 748
Bse1I ACTGG 3 cut(s) 136, 175, 507
Bse21I CCTNAGG 1 cut(s) 1272
Bse3DI GCAATG 2 cut(s) 1435, 1821
Bse8I GATNNNNATC 2 cut(s) 1347, 1869
BseBI CCWGG 4 cut(s) 675, 890, 1135, 1396
BseCI ATCGAT 2 cut(s) 732, 1010
BseDI CCNNGG 5 cut(s) 24, 592, 870, 1535, 1955
BseGI GGATG 5 cut(s) 246, 261, 805, 1036, 1922
BseJI GATNNNNATC 2 cut(s) 1347, 1869
BseLI CCNNNNNNNGG 5 cut(s) 110, 593, 876, 1478, 1915
BseMI GCAATG 2 cut(s) 1435, 1821
BseMII CTCAG 6 cut(s) 111, 476, 854, 1233, 1263, 1875
BseNI ACTGG 3 cut(s) 136, 175, 507
BseSI GKGCMC 2 cut(s) 627, 1112
BseXI GCAGC 5 cut(s) 708, 1323, 1412, 1624, 1699
BsgI GTGCAG 1 cut(s) 1698
Bsh1285I CGRYCG 4 cut(s) 151, 518, 553, 753
BshFI GGCC 8 cut(s) 29, 114, 294, 410, 625, 762, 1434, 1594
BshNI GGYRCC 1 cut(s) 740
BshTI ACCGGT 1 cut(s) 748
BshVI ATCGAT 2 cut(s) 732, 1010
BsiEI CGRYCG 4 cut(s) 151, 518, 553, 753
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 8 cut(s) 151, 553, 587, 708, 744, 749, 764, 774
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 5 cut(s) 110, 593, 876, 1478, 1915
BsmAI GTCTC 5 cut(s) 183, 228, 515, 532, 1462
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 8 cut(s) 29, 114, 294, 410, 625, 762, 1434, 1594
Bso31I GGTCTC 1 cut(s) 1462
Bsp119I TTCGAA 1 cut(s) 1208
Bsp120I GGGCCC 1 cut(s) 623
Bsp1286I GDGCHC 4 cut(s) 492, 627, 1112, 1535
Bsp143I GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
BspANI GGCC 8 cut(s) 29, 114, 294, 410, 625, 762, 1434, 1594
BspCNI CTCAG 6 cut(s) 110, 475, 853, 1234, 1264, 1874
BspDI ATCGAT 2 cut(s) 732, 1010
BspHI TCATGA 1 cut(s) 1867
BspLI GGNNCC 6 cut(s) 624, 625, 626, 742, 1532, 1944
BspPI GGATC 6 cut(s) 563, 664, 737, 1071, 1937, 1950
BspT104I TTCGAA 1 cut(s) 1208
BspT107I GGYRCC 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 1462
BsrDI GCAATG 2 cut(s) 1435, 1821
BsrFI RCCGGY 3 cut(s) 150, 586, 748
BsrI ACTGG 3 cut(s) 136, 175, 507
BssAI RCCGGY 3 cut(s) 150, 586, 748
BssECI CCNNGG 5 cut(s) 24, 592, 870, 1535, 1955
BssMI GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
BssNAI GTATAC 1 cut(s) 1152
BssNI GRCGYC 2 cut(s) 387, 741
BssT1I CCWWGG 2 cut(s) 870, 1535
Bst1107I GTATAC 1 cut(s) 1152
Bst2UI CCWGG 4 cut(s) 675, 890, 1135, 1396
Bst4CI ACNGT 3 cut(s) 782, 1360, 1411
Bst6I CTCTTC 1 cut(s) 1205
BstACI GRCGYC 2 cut(s) 387, 741
BstAPI GCANNNNNTGC 1 cut(s) 1811
BstBI TTCGAA 1 cut(s) 1208
BstC8I GCNNGC 4 cut(s) 156, 492, 830, 855
BstDEI CTNAG 7 cut(s) 97, 462, 840, 1038, 1242, 1272, 1861
BstDSI CCRYGG 3 cut(s) 24, 592, 1955
BstF5I GGATG 5 cut(s) 246, 261, 805, 1036, 1922
BstH2I RGCGCY 2 cut(s) 744, 770
BstHHI GCGC 2 cut(s) 743, 769
BstKTI GATC 9 cut(s) 361, 518, 558, 659, 732, 1066, 1867, 1873, 1945
BstMAI GTCTC 5 cut(s) 183, 228, 515, 532, 1462
BstMBI GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
BstMCI CGRYCG 4 cut(s) 151, 518, 553, 753
BstNI CCWGG 4 cut(s) 675, 890, 1135, 1396
BstNSI RCATGY 3 cut(s) 962, 1330, 1757
BstPAI GACNNNNGTC 1 cut(s) 438
BstSCI CCNGG 6 cut(s) 673, 707, 762, 888, 1133, 1394
BstSFI CTRYAG 1 cut(s) 1689
BstSLI GKGCMC 2 cut(s) 627, 1112
BstV1I GCAGC 5 cut(s) 708, 1323, 1412, 1624, 1699
BstX2I RGATCY 2 cut(s) 656, 1942
BstXI CCANNNNNNTGG 2 cut(s) 691, 1956
BstYI RGATCY 2 cut(s) 656, 1942
BstZ17I GTATAC 1 cut(s) 1152
Bsu15I ATCGAT 2 cut(s) 732, 1010
Bsu36I CCTNAGG 1 cut(s) 1272
BsuRI GGCC 8 cut(s) 29, 114, 294, 410, 625, 762, 1434, 1594
BsuTUI ATCGAT 2 cut(s) 732, 1010
BtgI CCRYGG 3 cut(s) 24, 592, 1955
BtrI CACGTC 2 cut(s) 280, 370
BtsCI GGATG 5 cut(s) 246, 261, 805, 1036, 1922
BtsI GCAGTG 2 cut(s) 1392, 1986
BtsIMutI CAGTG 2 cut(s) 1392, 1986
Cac8I GCNNGC 4 cut(s) 156, 492, 830, 855
CaiI CAGNNNCTG 1 cut(s) 1811
CciI TCATGA 1 cut(s) 1867
CfoI GCGC 2 cut(s) 743, 769
Cfr10I RCCGGY 3 cut(s) 150, 586, 748
ClaI ATCGAT 2 cut(s) 732, 1010
CpoI CGGWCCG 2 cut(s) 715, 746
CseI GACGC 2 cut(s) 395, 1643
CsiI ACCWGGT 1 cut(s) 888
Csp6I GTAC 3 cut(s) 1625, 1766, 1789
CspAI ACCGGT 1 cut(s) 748
CspI CGGWCCG 2 cut(s) 715, 746
CviAII CATG 9 cut(s) 13, 959, 1327, 1439, 1566, 1718, 1754, 1868, 1901
CviQI GTAC 3 cut(s) 1625, 1766, 1789
DdeI CTNAG 7 cut(s) 97, 462, 840, 1038, 1242, 1272, 1861
DinI GGCGCC 1 cut(s) 742
DpnI GATC 9 cut(s) 360, 517, 557, 658, 731, 1065, 1866, 1872, 1944
DpnII GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
DraIII CACNNNGTG 2 cut(s) 687, 1721
EaeI YGGCCR 3 cut(s) 27, 408, 1432
Eam1104I CTCTTC 1 cut(s) 1205
EarI CTCTTC 1 cut(s) 1205
EciI GGCGGA 6 cut(s) 10, 185, 272, 304, 331, 872
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 870, 1535
Eco24I GRGCYC 3 cut(s) 492, 627, 1535
Eco31I GGTCTC 1 cut(s) 1462
Eco47I GGWCC 7 cut(s) 589, 705, 715, 746, 1020, 1392, 1857
Eco53kI GAGCTC 1 cut(s) 490
Eco81I CCTNAGG 1 cut(s) 1272
EcoICRI GAGCTC 1 cut(s) 490
EcoO109I RGGNCCY 4 cut(s) 623, 624, 1020, 1857
EcoRI GAATTC 2 cut(s) 915, 934
EcoRII CCWGG 4 cut(s) 673, 888, 1133, 1394
EcoT14I CCWWGG 2 cut(s) 870, 1535
EcoT38I GRGCYC 3 cut(s) 492, 627, 1535
EgeI GGCGCC 1 cut(s) 742
EheI GGCGCC 1 cut(s) 742
ErhI CCWWGG 2 cut(s) 870, 1535
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 9 cut(s) 16, 962, 1330, 1442, 1569, 1721, 1757, 1871, 1904
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FaqI GGGAC 1 cut(s) 124
FatI CATG 9 cut(s) 12, 958, 1326, 1438, 1565, 1717, 1753, 1867, 1900
FauI CCCGC 4 cut(s) 149, 388, 511, 1255
FauNDI CATATG 1 cut(s) 1521
FblI GTMKAC 2 cut(s) 753, 1151
Fnu4HI GCNGC 8 cut(s) 521, 697, 816, 1337, 1426, 1435, 1638, 1713
FokI GGATG 5 cut(s) 233, 248, 792, 1043, 1909
FriOI GRGCYC 3 cut(s) 492, 627, 1535
Fsp4HI GCNGC 8 cut(s) 521, 697, 816, 1337, 1426, 1435, 1638, 1713
FspBI CTAG 4 cut(s) 68, 479, 1536, 2013
GlaI GCGC 2 cut(s) 742, 768
GluI GCNGC 8 cut(s) 521, 697, 816, 1337, 1426, 1435, 1638, 1713
HaeII RGCGCY 2 cut(s) 744, 770
HaeIII GGCC 8 cut(s) 29, 114, 294, 410, 625, 762, 1434, 1594
HapII CCGG 8 cut(s) 151, 553, 587, 708, 744, 749, 764, 774
HgaI GACGC 2 cut(s) 395, 1643
HhaI GCGC 2 cut(s) 743, 769
Hin1I GRCGYC 2 cut(s) 387, 741
Hin1II CATG 9 cut(s) 16, 962, 1330, 1442, 1569, 1721, 1757, 1871, 1904
Hin6I GCGC 2 cut(s) 741, 767
HinP1I GCGC 2 cut(s) 741, 767
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HpaII CCGG 8 cut(s) 151, 553, 587, 708, 744, 749, 764, 774
Hpy166II GTNNAC 5 cut(s) 592, 601, 754, 1152, 1446
Hpy188III TCNNGA 7 cut(s) 574, 1081, 1271, 1700, 1738, 1868, 2013
Hpy8I GTNNAC 5 cut(s) 592, 601, 754, 1152, 1446
Hpy99I CGWCG 6 cut(s) 293, 389, 437, 536, 782, 1159
HpyAV CCTTC 9 cut(s) 133, 181, 306, 310, 315, 343, 838, 1624, 2000
HpyCH4III ACNGT 3 cut(s) 782, 1360, 1411
HpyCH4IV ACGT 7 cut(s) 279, 369, 435, 1154, 1181, 1550, 1744
HpyCH4V TGCA 7 cut(s) 823, 1219, 1385, 1428, 1715, 1814, 1979
HpyF3I CTNAG 7 cut(s) 97, 462, 840, 1038, 1242, 1272, 1861
HpySE526I ACGT 7 cut(s) 279, 369, 435, 1154, 1181, 1550, 1744
Hsp92I GRCGYC 2 cut(s) 387, 741
Hsp92II CATG 9 cut(s) 16, 962, 1330, 1442, 1569, 1721, 1757, 1871, 1904
HspAI GCGC 2 cut(s) 741, 767
KasI GGCGCC 1 cut(s) 740
Kzo9I GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
LmnI GCTCC 3 cut(s) 1459, 1530, 1740
Lsp1109I GCAGC 5 cut(s) 708, 1323, 1412, 1624, 1699
LweI GCATC 2 cut(s) 1228, 1931
MabI ACCWGGT 1 cut(s) 888
MaeI CTAG 4 cut(s) 68, 479, 1536, 2013
MaeII ACGT 7 cut(s) 279, 369, 435, 1154, 1181, 1550, 1744
MaeIII GTNAC 3 cut(s) 440, 662, 1818
MalI GATC 9 cut(s) 360, 517, 557, 658, 731, 1065, 1866, 1872, 1944
MboI GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
MboII GAAGA 4 cut(s) 887, 1222, 1289, 1774
MflI RGATCY 2 cut(s) 656, 1942
MhlI GDGCHC 4 cut(s) 492, 627, 1112, 1535
MluCI AATT 9 cut(s) 105, 915, 934, 1084, 1144, 1669, 1777, 1913, 2048
Mly113I GGCGCC 1 cut(s) 741
MlyI GAGTC 7 cut(s) 161, 389, 536, 659, 1229, 1510, 1576
MmeI TCCRAC 3 cut(s) 186, 417, 693
MroXI GAANNNNTTC 1 cut(s) 1781
MseI TTAA 1 cut(s) 1143
MslI CAYNNNNRTG 3 cut(s) 11, 963, 1954
MspI CCGG 8 cut(s) 151, 553, 587, 708, 744, 749, 764, 774
MspR9I CCNGG 6 cut(s) 675, 709, 764, 890, 1135, 1396
MvaI CCWGG 4 cut(s) 675, 890, 1135, 1396
NarI GGCGCC 1 cut(s) 741
NciI CCSGG 2 cut(s) 709, 764
NdeI CATATG 1 cut(s) 1521
NdeII GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
NlaIII CATG 9 cut(s) 16, 962, 1330, 1442, 1569, 1721, 1757, 1871, 1904
NlaIV GGNNCC 6 cut(s) 624, 625, 626, 742, 1532, 1944
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 3 cut(s) 440, 662, 1818
NspI RCATGY 3 cut(s) 962, 1330, 1757
NspV TTCGAA 1 cut(s) 1208
PagI TCATGA 1 cut(s) 1867
PciI ACATGT 2 cut(s) 958, 1753
PcsI WCGNNNNNNNCGW 6 cut(s) 285, 546, 1109, 1145, 1154, 1187
PdmI GAANNNNTTC 1 cut(s) 1781
PfeI GAWTC 3 cut(s) 986, 1205, 1703
PflFI GACNNNGTC 2 cut(s) 709, 782
PflMI CCANNNNNTGG 2 cut(s) 1478, 1915
PinAI ACCGGT 1 cut(s) 748
PkrI GCNGC 8 cut(s) 522, 698, 817, 1338, 1427, 1436, 1639, 1714
Ple19I CGATCG 1 cut(s) 518
PleI GAGTC 7 cut(s) 161, 389, 536, 659, 1229, 1510, 1576
PluTI GGCGCC 1 cut(s) 744
PpsI GAGTC 7 cut(s) 161, 389, 536, 659, 1229, 1510, 1576
PpuMI RGGWCCY 2 cut(s) 1020, 1857
PscI ACATGT 2 cut(s) 958, 1753
PshAI GACNNNNGTC 1 cut(s) 438
PshBI ATTAAT 1 cut(s) 1143
PsiI TTATAA 1 cut(s) 1089
Psp124BI GAGCTC 1 cut(s) 492
Psp5II RGGWCCY 2 cut(s) 1020, 1857
Psp6I CCWGG 4 cut(s) 673, 888, 1133, 1394
PspGI CCWGG 4 cut(s) 673, 888, 1133, 1394
PspN4I GGNNCC 6 cut(s) 624, 625, 626, 742, 1532, 1944
PspOMI GGGCCC 1 cut(s) 623
PspPPI RGGWCCY 2 cut(s) 1020, 1857
PstNI CAGNNNCTG 1 cut(s) 1811
PsuI RGATCY 2 cut(s) 656, 1942
PsyI GACNNNGTC 2 cut(s) 709, 782
PvuI CGATCG 1 cut(s) 518
RsaI GTAC 3 cut(s) 1626, 1767, 1790
RsaNI GTAC 3 cut(s) 1625, 1766, 1789
RseI CAYNNNNRTG 3 cut(s) 11, 963, 1954
Rsr2I CGGWCCG 2 cut(s) 715, 746
RsrII CGGWCCG 2 cut(s) 715, 746
SacI GAGCTC 1 cut(s) 492
SalI GTCGAC 1 cut(s) 752
SaqAI TTAA 1 cut(s) 1143
SatI GCNGC 8 cut(s) 521, 697, 816, 1337, 1426, 1435, 1638, 1713
Sau3AI GATC 9 cut(s) 358, 515, 555, 656, 729, 1063, 1864, 1870, 1942
SchI GAGTC 7 cut(s) 161, 389, 536, 659, 1229, 1510, 1576
ScrFI CCNGG 6 cut(s) 675, 709, 764, 890, 1135, 1396
SduI GDGCHC 4 cut(s) 492, 627, 1112, 1535
SexAI ACCWGGT 1 cut(s) 888
SfaNI GCATC 2 cut(s) 1228, 1931
SfcI CTRYAG 1 cut(s) 1689
SfoI GGCGCC 1 cut(s) 742
SfuI TTCGAA 1 cut(s) 1208
SinI GGWCC 7 cut(s) 589, 705, 715, 746, 1020, 1392, 1857
SmiMI CAYNNNNRTG 3 cut(s) 11, 963, 1954
SmlI CTYRAG 1 cut(s) 1463
SmoI CTYRAG 1 cut(s) 1463
Sse9I AATT 9 cut(s) 105, 915, 934, 1084, 1144, 1669, 1777, 1913, 2048
SspDI GGCGCC 1 cut(s) 740
SspMI CTAG 4 cut(s) 68, 479, 1536, 2013
SstI GAGCTC 1 cut(s) 492
StyD4I CCNGG 6 cut(s) 673, 707, 762, 888, 1133, 1394
StyI CCWWGG 2 cut(s) 870, 1535
TaaI ACNGT 3 cut(s) 782, 1360, 1411
TaiI ACGT 7 cut(s) 282, 372, 438, 1157, 1184, 1553, 1747
TasI AATT 9 cut(s) 105, 915, 934, 1084, 1144, 1669, 1777, 1913, 2048
TatI WGTACW 1 cut(s) 1788
TauI GCSGC 3 cut(s) 523, 818, 1437
TfiI GAWTC 3 cut(s) 986, 1205, 1703
Tru1I TTAA 1 cut(s) 1143
Tru9I TTAA 1 cut(s) 1143
TscAI CASTG 2 cut(s) 1392, 1986
TseFI GTSAC 3 cut(s) 440, 662, 1818
TseI GCWGC 5 cut(s) 696, 1336, 1425, 1637, 1712
Tsp45I GTSAC 3 cut(s) 440, 662, 1818
TspDTI ATGAA 3 cut(s) 794, 1110, 1599
TspGWI ACGGA 2 cut(s) 520, 1944
TspRI CASTG 2 cut(s) 1392, 1986
Tth111I GACNNNGTC 2 cut(s) 709, 782
Van91I CCANNNNNTGG 2 cut(s) 1478, 1915
VpaK11BI GGWCC 7 cut(s) 589, 705, 715, 746, 1020, 1392, 1857
VspI ATTAAT 1 cut(s) 1143
XapI RAATTY 3 cut(s) 915, 934, 1777
XbaI TCTAGA 1 cut(s) 2012
XceI RCATGY 3 cut(s) 962, 1330, 1757
XcmI CCANNNNNNNNNTGG 1 cut(s) 1953
XmaJI CCTAGG 1 cut(s) 1535
XmiI GTMKAC 2 cut(s) 753, 1151
XmnI GAANNNNTTC 1 cut(s) 1781
XspI CTAG 4 cut(s) 68, 479, 1536, 2013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.