Rorug03G0193900

Zinc finger AN1 and C2H2 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009954
Physical Location & Seq
Forward (+)
17027563 .. 17032597
5035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0193900.1

Sequence Viewer

Length: 3723 bp
ATGGTGTTGGCGATTCTTGTGGTAACTGTGACATGGATCGGTGTCCTAGCCAGTCTTAAGTGGATATGGAAGAGTTGGTTCAGTTACATGGTTGAAAAGCCAGAGCCAATATCAGAGGATAATCAAGAACCGGTTGACGTCGATGCCTCTGCATCTCTTCCTTCATCATCAGCTCCTAGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGGAGACACTCGCAAAGGTATTGCATTCGAATTATACGATCGACTGCAAAACAGGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCCAAACTATGCCACTTCCACTTGGTGTTTGGAGGAACTTAGATACATTTGTGAATGCATGGAAGAAGACAACAACAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGCAGTTTCGGAGATGCTTTCACTAAGCATCAAAAATCTGGGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGGTGGCATACACTGAATTATAAAACTGAAAGAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGATCTACTGAACATGAGTTTAAAATGTCCATCGGAGGTTTTGAAGTATTTGAAGCAACAAAACAAGTCATTGATAAAGTCATGAATGAGCTAAAAGACGATGAGGCCACTACCATTGGGATCTACGGAATGGGGGGCGTTGGAAAGACGACAATGGTGAAATATGTTGGAGCACAAGCCCAAGAAAGCAGGCTTTTTAATCAAGTGATTATGGTTGTCGTATCCCAAAACCCCGACTTGATGAAAGTTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTCAAGGAGGAGACAGAAATTGGAAGAGCCATTAAATTGAAGGAGAAGATAATGAGAGGAACTGGAATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGCATTGGAATTCCGAGCCACAACGAACTTCAAAGATGCAAATCCAAAGTTCTACTAACCACCAGAAAATTGAGTGTTTGCCATTCCATGGATTGCCATGCAAACATACATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAAGGAAGTCTTTTGACAAATCAACCAATTTCTATGATGTAGCACGTAAGGTGGCTAGAGAATGCGCCGGTCTACCAGTTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGATGGATGGAAAGAAGCTGCTCGACGACTAAAAGCGTCTCAACCTGCCAACCCTGAAGATGAGGGAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAACACTTGCTCATGTATAGCATTGGGAAAGGAATGTTTCAAGATTCAAACATGCTAGAAGCCCGAGCGACAACATACTTAGTGGTGAAGGCCCTTAAAGATTCTTGCTTGCTTTTGGACGCTGGAGATGACAAATGTGTAAGGATGCATGATGTCATTCGGGATATGGCAATACGAATTTCCTCATCTGAAGACGGCCAGAGGTTCTTGGTGAAAGTTGGCTGTGAATTGAAGAATTGGCCAAGGATTGATGTACATAAAGGCTACTCTGCAATCTCACTAATGATGAACGGAATTTGCAAGCTACCCGAAGAGTTGGTATGTCAAAATCTCCAGATTTTATTACTACAACACAATGCTTCATTAAATGATATCCCTAACTCTTTCTTCCAAAGTCAGAAAGAATTAAGAGTCTTGGATCTTAGCCGCACTGGTATTTCACTACTACCCCAATCAATCGGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCGGCAACAAAGTTGACACTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAATGAGAGGAACTTCTCTGAAAGACTTGTCAAGAGAAATAGGACAATTGACCAATCTAAGGATGCTCGATATAAGTGCTGCAGCTTTTCCCATGAGAGGAAGTATTTGCACAATTCCATCTAAATTGATATCAAAGTTGCATAAATTAGAAGAGCTGTACATGCAATGTGGATTTTGGGACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTAAGCATTTTGAAAGTTTGCATATTTGATGCAAATTGCATCCCTAAAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTCGATATTATTATTTGCAGCGGCCATAATGAAGAGAGAGTCATTAAGAGCCCACAATTTGGTCATAATTGTAGATCCTTGAGTCTTGATAGTACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACACTGTGGTAAAGAACACTGAGAGGCTAAGTTATAGAGAATGCAGAGGGTTGAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTTCATGGACTGAAAGTTCTCTCTGTAGAAGGTCGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATATGCCTGTGTTTGAGAACTTGGAAGAGTTGGTACTGTTTAGGCTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGGAGGTCCGTCAGTGTCGTAACTTGAGGAATGTACTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAAAACTATACTGTGATGGAGGTAAAGTGGATCATGTGTTTGAATGTAAAGGATTTGAGCCAGATCAAACAAATCTGAGGGAGATGGAGTTGCGAGATCTAGATGTAGTACGAAGCATATGTAATGGTCCTGTTCCACGCGGAATGTTCCAGGCTCTAAAGCAATTGGTCATTTACAAGTGCAACTTCCAAGGAAGTCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAAACCTTACTGTATCCGAATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAACAACAAGAAGACCGTTGTTCCAAAATTGAAGAACCTAAATTTGGAAAATCTTCCAATGTTGTACGAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAAACCTGAGTCTGTTTGGCTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCGTTTTCAATGATGCTCTTCGTCATTCTCTGCTACTCAGCAAACTAGGGTATAACGAACTGAAGCTTCCAGAATCGACGACTCTCAAGCCAATCACTCCTGATTTGTCCTCAACCATCCGAATTTCAGTGGCCGGAAGCTTGATGAAAGCAGTGATCAGACCTGGTGGAGGTGATTCAACACCTTCAGATGGTGATCAGCTCATATATCATTGCATTGTTAGAGCATTGGATGGAGTTATTGTTGAATCTTCAAGATCAGAATTTAGTGCCTTCTATTACAGCGAGAGTGCTGTTCGTTTAGTAATGGGAAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-AN1 zf-C2H2 zf-C2H2_4

Protein Analysis

1240

Amino Acids

140.35

Weight (kDa)

5.88

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 62 - 235 7.2e-51 TIR domain
TIR_2 PF13676 65 - 162 5.8e-17 TIR domain
NB-ARC PF00931 250 - 415 3.6e-31 NB-ARC domain
LRR_8 PF13855 622 - 679 3.5e-08 Leucine rich repeat
LRR_14 PF23598 646 - 791 3e-15 Leucine-rich repeat region
LRR_RPS2 PF23247 919 - 1059 9.3e-22 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 998 - 1120 4e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AasI GACNNNNNNGTC 1 cut(s) 2558
AatII GACGTC 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 1414
AccB7I CCANNNNNTGG 2 cut(s) 1168, 2489
AccI GTMKAC 1 cut(s) 1312
AccII CGCG 1 cut(s) 3057
AccIII TCCGGA 1 cut(s) 2548
AciI CCGC 4 cut(s) 1963, 2037, 2451, 3057
AclWI GGATC 8 cut(s) 44, 476, 489, 815, 1962, 2499, 2827, 2955
AcoI YGGCCR 4 cut(s) 1732, 1775, 2452, 3534
AcsI RAATTY 9 cut(s) 456, 604, 968, 1089, 1713, 1830, 3259, 3525, 3665
AcuI CTGAAG 5 cut(s) 1437, 1746, 2807, 3485, 3573
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 402
AfaI GTAC 8 cut(s) 495, 1791, 2258, 2524, 2766, 2877, 3027, 3284
AfiI CCNNNNNNNGG 8 cut(s) 1168, 2157, 2195, 2489, 3184, 3262, 3572, 3593
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 2720
AgeI ACCGGT 1 cut(s) 130
AjnI CCWGG 3 cut(s) 2815, 3066, 3565
AjuI GAANNNNNNNTTGG 6 cut(s) 1475, 1507, 2304, 2336, 3245, 3277
AloI GAACNNNNNNTCC 2 cut(s) 2649, 2681
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 5 cut(s) 210, 935, 974, 1404, 3119
AlwI GGATC 8 cut(s) 44, 476, 489, 815, 1962, 2499, 2827, 2955
Ama87I CYCGRG 1 cut(s) 1599
Aor13HI TCCGGA 1 cut(s) 2548
AoxI GGCC 7 cut(s) 792, 1341, 1626, 1732, 1775, 2452, 3534
ApaI GGGCCC 1 cut(s) 1345
ApeKI GCWGC 7 cut(s) 571, 1379, 1503, 2177, 2180, 2448, 3345
ApoI RAATTY 9 cut(s) 456, 604, 968, 1089, 1713, 1830, 3259, 3525, 3665
ArsI GACNNNNNNTTYG 2 cut(s) 3165, 3197
AsiGI ACCGGT 1 cut(s) 130
Asp700I GAANNNNTTC 1 cut(s) 3270
AspA2I CCTAGG 1 cut(s) 176
AspLEI GCGC 1 cut(s) 1307
AspS9I GGNCC 5 cut(s) 1341, 1342, 1627, 2848, 3044
AsuHPI GGTGA 6 cut(s) 856, 1633, 1759, 2816, 3587, 3608
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 1599
AvaII GGWCC 2 cut(s) 2848, 3044
AvrII CCTAGG 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 1345
BaeI ACNNNNGTAYC 2 cut(s) 412, 445
BalI TGGCCA 1 cut(s) 1777
BamHI GGATCC 1 cut(s) 481
BanII GRGCYC 2 cut(s) 1345, 2483
BarI GAAGNNNNNNTAC 2 cut(s) 2748, 2780
BbsI GAAGAC 3 cut(s) 450, 1734, 3236
Bbv12I GWGCWC 1 cut(s) 862
BbvI GCAGC 7 cut(s) 583, 1366, 1490, 2164, 2192, 2460, 3332
BceAI ACGGC 2 cut(s) 354, 1747
BcgI CGANNNNNNTGC 6 cut(s) 131, 165, 2156, 2190, 3275, 3309
BciT130I CCWGG 3 cut(s) 2817, 3068, 3567
BciVI GTATCC 2 cut(s) 919, 3178
BclI TGATCA 2 cut(s) 3558, 3598
BcoDI GTCTC 5 cut(s) 210, 935, 974, 1404, 3119
BfaI CTAG 6 cut(s) 47, 177, 1296, 1592, 3017, 3449
BfmI CTRYAG 3 cut(s) 2178, 2673, 2827
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 1414
BfuI GTATCC 2 cut(s) 919, 3178
BglII AGATCT 1 cut(s) 3013
BlnI CCTAGG 1 cut(s) 176
BmcAI AGTACT 1 cut(s) 2524
Bme1390I CCNGG 3 cut(s) 2817, 3068, 3567
Bme18I GGWCC 2 cut(s) 2848, 3044
BmeT110I CYCGRG 1 cut(s) 1599
BmgT120I GGNCC 5 cut(s) 1341, 1342, 1627, 2848, 3044
BmiI GGNNCC 4 cut(s) 483, 1343, 2542, 3398
BmrFI CCNGG 3 cut(s) 2817, 3068, 3567
BmrI ACTGGG 1 cut(s) 2290
BmuI ACTGGG 1 cut(s) 2290
BpiI GAAGAC 3 cut(s) 450, 1734, 3236
BpmI CTGGAG 2 cut(s) 1680, 1853
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 4 cut(s) 2102, 2530, 2887, 3473
BsaAI YACGTR 1 cut(s) 1286
BsaBI GATNNNNATC 2 cut(s) 1120, 3597
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 5 cut(s) 176, 1167, 1778, 2641, 3106
BsaWI WCCGGW 2 cut(s) 130, 2548
BsaXI ACNNNNNCTCC 6 cut(s) 2928, 2958, 2990, 3020, 3630, 3660
Bsc4I CCNNNNNNNGG 8 cut(s) 1168, 2157, 2195, 2489, 3184, 3262, 3572, 3593
Bse118I RCCGGY 2 cut(s) 130, 1307
Bse1I ACTGG 6 cut(s) 51, 1036, 1316, 1972, 2285, 2558
Bse3DI GCAATG 2 cut(s) 2270, 3613
Bse8I GATNNNNATC 2 cut(s) 1120, 3597
BseAI TCCGGA 1 cut(s) 2548
BseBI CCWGG 3 cut(s) 2817, 3068, 3567
BseDI CCNNGG 5 cut(s) 176, 1167, 1778, 2641, 3106
BseJI GATNNNNATC 2 cut(s) 1120, 3597
BseLI CCNNNNNNNGG 8 cut(s) 1168, 2157, 2195, 2489, 3184, 3262, 3572, 3593
BseMI GCAATG 2 cut(s) 2270, 3613
BseMII CTCAG 5 cut(s) 2574, 2984, 3149, 3323, 3454
BseNI ACTGG 6 cut(s) 51, 1036, 1316, 1972, 2285, 2558
BseRI GAGGAG 1 cut(s) 992
BseSI GKGCMC 1 cut(s) 1345
BseXI GCAGC 7 cut(s) 583, 1366, 1490, 2164, 2192, 2460, 3332
BseYI CCCAGC 1 cut(s) 958
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 3057
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 7 cut(s) 794, 1343, 1628, 1734, 1777, 2454, 3536
BshTI ACCGGT 1 cut(s) 130
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 862
BsiHKCI CYCGRG 1 cut(s) 1599
BsiSI CCGG 4 cut(s) 131, 1308, 2549, 3537
BslFI GGGAC 1 cut(s) 2291
BslI CCNNNNNNNGG 8 cut(s) 1168, 2157, 2195, 2489, 3184, 3262, 3572, 3593
BsmAI GTCTC 5 cut(s) 210, 935, 974, 1404, 3119
BsmBI CGTCTC 1 cut(s) 1404
BsmFI GGGAC 1 cut(s) 2291
BsmI GAATGC 6 cut(s) 236, 437, 1307, 2609, 2716, 3179
BsnI GGCC 7 cut(s) 794, 1343, 1628, 1734, 1777, 2454, 3536
BsoBI CYCGRG 1 cut(s) 1599
Bsp119I TTCGAA 1 cut(s) 240
Bsp120I GGGCCC 1 cut(s) 1341
Bsp1286I GDGCHC 3 cut(s) 862, 1345, 2483
Bsp13I TCCGGA 1 cut(s) 2548
Bsp1407I TGTACA 2 cut(s) 1789, 2256
Bsp19I CCATGG 1 cut(s) 1167
BspACI CCGC 4 cut(s) 1963, 2037, 2451, 3057
BspANI GGCC 7 cut(s) 794, 1343, 1628, 1734, 1777, 2454, 3536
BspCNI CTCAG 5 cut(s) 2575, 2985, 3148, 3324, 3453
BspEI TCCGGA 1 cut(s) 2548
BspFNI CGCG 1 cut(s) 3057
BspHI TCATGA 1 cut(s) 768
BspLI GGNNCC 4 cut(s) 483, 1343, 2542, 3398
BspMAI CTGCAG 2 cut(s) 2182, 2831
BspMI ACCTGC 1 cut(s) 1414
BspPI GGATC 8 cut(s) 44, 476, 489, 815, 1962, 2499, 2827, 2955
BspQI GCTCTTC 4 cut(s) 500, 988, 2244, 3426
BspT104I TTCGAA 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 2270, 3613
BsrFI RCCGGY 2 cut(s) 130, 1307
BsrGI TGTACA 2 cut(s) 1789, 2256
BsrI ACTGG 6 cut(s) 51, 1036, 1316, 1972, 2285, 2558
BssAI RCCGGY 2 cut(s) 130, 1307
BssECI CCNNGG 5 cut(s) 176, 1167, 1778, 2641, 3106
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 4 cut(s) 176, 1167, 1778, 3106
Bst2UI CCWGG 3 cut(s) 2817, 3068, 3567
Bst4CI ACNGT 8 cut(s) 28, 2569, 2642, 2769, 2880, 2930, 3166, 3235
BstACI GRCGYC 1 cut(s) 138
BstAFI CTTAAG 1 cut(s) 56
BstAUI TGTACA 2 cut(s) 1789, 2256
BstBAI YACGTR 1 cut(s) 1286
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 3 cut(s) 878, 1646, 1838
BstDSI CCRYGG 2 cut(s) 1167, 2641
BstENI CCTNNNNNAGG 1 cut(s) 3570
BstFNI CGCG 1 cut(s) 3057
BstHHI GCGC 1 cut(s) 1307
BstMAI GTCTC 5 cut(s) 210, 935, 974, 1404, 3119
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 2 cut(s) 1328, 2260
BstNI CCWGG 3 cut(s) 2817, 3068, 3567
BstNSI RCATGY 3 cut(s) 1591, 2263, 2724
BstSCI CCNGG 3 cut(s) 2815, 3066, 3565
BstSFI CTRYAG 3 cut(s) 2178, 2673, 2827
BstSLI GKGCMC 1 cut(s) 1345
BstUI CGCG 1 cut(s) 3057
BstV1I GCAGC 7 cut(s) 583, 1366, 1490, 2164, 2192, 2460, 3332
BstV2I GAAGAC 3 cut(s) 450, 1734, 3236
BstX2I RGATCY 6 cut(s) 481, 807, 1954, 2504, 2819, 3013
BstYI RGATCY 6 cut(s) 481, 807, 1954, 2504, 2819, 3013
BsuI GTATCC 2 cut(s) 919, 3178
BsuRI GGCC 7 cut(s) 794, 1343, 1628, 1734, 1777, 2454, 3536
BtgI CCRYGG 2 cut(s) 1167, 2641
BtsI GCAGTG 2 cut(s) 581, 3561
BtsIMutI CAGTG 9 cut(s) 581, 623, 1965, 2565, 2580, 2861, 3143, 3537, 3561
BveI ACCTGC 1 cut(s) 1414
Cac8I GCNNGC 3 cut(s) 878, 1646, 1838
CciI TCATGA 1 cut(s) 768
CfoI GCGC 1 cut(s) 1307
Cfr10I RCCGGY 2 cut(s) 130, 1307
Cfr13I GGNCC 5 cut(s) 1341, 1342, 1627, 2848, 3044
CseI GACGC 2 cut(s) 1386, 1664
CsiI ACCWGGT 1 cut(s) 3565
Csp6I GTAC 8 cut(s) 494, 1790, 2257, 2523, 2765, 2876, 3026, 3283
CspAI ACCGGT 1 cut(s) 130
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviQI GTAC 8 cut(s) 494, 1790, 2257, 2523, 2765, 2876, 3026, 3283
DraI TTTAAA 3 cut(s) 591, 709, 967
DraIII CACNNNGTG 1 cut(s) 402
DrdI GACNNNNNNGTC 1 cut(s) 2558
DseDI GACNNNNNNGTC 1 cut(s) 2558
EaeI YGGCCR 4 cut(s) 1732, 1775, 2452, 3534
Eco130I CCWWGG 4 cut(s) 176, 1167, 1778, 3106
Eco24I GRGCYC 2 cut(s) 1345, 2483
Eco32I GATATC 4 cut(s) 500, 1528, 1909, 2229
Eco47I GGWCC 2 cut(s) 2848, 3044
Eco57I CTGAAG 5 cut(s) 1437, 1746, 2807, 3485, 3573
Eco88I CYCGRG 1 cut(s) 1599
EcoNI CCTNNNNNAGG 1 cut(s) 3570
EcoO109I RGGNCCY 3 cut(s) 1341, 1342, 1627
EcoRI GAATTC 2 cut(s) 456, 1089
EcoRII CCWGG 3 cut(s) 2815, 3066, 3565
EcoRV GATATC 4 cut(s) 500, 1528, 1909, 2229
EcoT14I CCWWGG 4 cut(s) 176, 1167, 1778, 3106
EcoT22I ATGCAT 3 cut(s) 437, 1445, 1686
EcoT38I GRGCYC 2 cut(s) 1345, 2483
ErhI CCWWGG 4 cut(s) 176, 1167, 1778, 3106
Esp3I CGTCTC 1 cut(s) 1404
FalI AAGNNNNNCTT 4 cut(s) 1234, 1266, 3086, 3118
FaqI GGGAC 1 cut(s) 2291
FauNDI CATATG 1 cut(s) 3035
FbaI TGATCA 2 cut(s) 3558, 3598
FblI GTMKAC 1 cut(s) 1312
FriOI GRGCYC 2 cut(s) 1345, 2483
FspBI CTAG 6 cut(s) 47, 177, 1296, 1592, 3017, 3449
GlaI GCGC 1 cut(s) 1306
GsaI CCCAGC 1 cut(s) 962
GsuI CTGGAG 2 cut(s) 1680, 1853
HaeIII GGCC 7 cut(s) 794, 1343, 1628, 1734, 1777, 2454, 3536
HapII CCGG 4 cut(s) 131, 1308, 2549, 3537
HgaI GACGC 2 cut(s) 1386, 1664
HhaI GCGC 1 cut(s) 1307
Hin1I GRCGYC 1 cut(s) 138
Hin6I GCGC 1 cut(s) 1305
HinP1I GCGC 1 cut(s) 1305
HincII GTYRAC 3 cut(s) 136, 2050, 2563
HindII GTYRAC 3 cut(s) 136, 2050, 2563
HindIII AAGCTT 4 cut(s) 2016, 2078, 3467, 3541
HpaII CCGG 4 cut(s) 131, 1308, 2549, 3537
HphI GGTGA 6 cut(s) 856, 1633, 1759, 2816, 3587, 3608
Hpy166II GTNNAC 5 cut(s) 136, 1313, 2050, 2428, 2563
Hpy8I GTNNAC 5 cut(s) 136, 1313, 2050, 2428, 2563
Hpy99I CGWCG 3 cut(s) 143, 1389, 3484
HpyCH4III ACNGT 8 cut(s) 28, 2569, 2642, 2769, 2880, 2930, 3166, 3235
HpyCH4IV ACGT 2 cut(s) 138, 1285
HpyF10VI GCNNNNNNNGC 2 cut(s) 1328, 2260
HpySE526I ACGT 2 cut(s) 138, 1285
Hsp92I GRCGYC 1 cut(s) 138
HspAI GCGC 1 cut(s) 1305
Kpn2I TCCGGA 1 cut(s) 2548
Ksp22I TGATCA 2 cut(s) 3558, 3598
LguI GCTCTTC 4 cut(s) 500, 988, 2244, 3426
LmnI GCTCC 3 cut(s) 178, 857, 1221
Lsp1109I GCAGC 7 cut(s) 583, 1366, 1490, 2164, 2192, 2460, 3332
MabI ACCWGGT 1 cut(s) 3565
MaeI CTAG 6 cut(s) 47, 177, 1296, 1592, 3017, 3449
MaeII ACGT 2 cut(s) 138, 1285
MaeIII GTNAC 6 cut(s) 22, 28, 83, 2618, 2808, 2861
MfeI CAATTG 4 cut(s) 1533, 2144, 3080, 3149
MflI RGATCY 6 cut(s) 481, 807, 1954, 2504, 2819, 3013
MhlI GDGCHC 3 cut(s) 862, 1345, 2483
MlsI TGGCCA 1 cut(s) 1777
MluNI TGGCCA 1 cut(s) 1777
MlyI GAGTC 5 cut(s) 1956, 2478, 2521, 3343, 3478
MmeI TCCRAC 2 cut(s) 808, 835
Mox20I TGGCCA 1 cut(s) 1777
Mph1103I ATGCAT 3 cut(s) 437, 1445, 1686
MroI TCCGGA 1 cut(s) 2548
MroXI GAANNNNTTC 1 cut(s) 3270
MscI TGGCCA 1 cut(s) 1777
MslI CAYNNNNRTG 1 cut(s) 2627
Msp20I TGGCCA 1 cut(s) 1777
MspA1I CMGCKG 1 cut(s) 2451
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 4 cut(s) 131, 1308, 2549, 3537
MspR9I CCNGG 3 cut(s) 2817, 3068, 3567
MunI CAATTG 4 cut(s) 1533, 2144, 3080, 3149
Mva1269I GAATGC 6 cut(s) 236, 437, 1307, 2609, 2716, 3179
MvaI CCWGG 3 cut(s) 2817, 3068, 3567
MvnI CGCG 1 cut(s) 3057
MwoI GCNNNNNNNGC 2 cut(s) 1328, 2260
NcoI CCATGG 1 cut(s) 1167
NdeI CATATG 1 cut(s) 3035
NlaIV GGNNCC 4 cut(s) 483, 1343, 2542, 3398
NmuCI GTSAC 1 cut(s) 28
NsiI ATGCAT 3 cut(s) 437, 1445, 1686
NspI RCATGY 3 cut(s) 1591, 2263, 2724
NspV TTCGAA 1 cut(s) 240
PagI TCATGA 1 cut(s) 768
PciI ACATGT 1 cut(s) 2720
PciSI GCTCTTC 4 cut(s) 500, 988, 2244, 3426
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 6 cut(s) 236, 437, 1307, 2609, 2716, 3179
PdmI GAANNNNTTC 1 cut(s) 3270
PflMI CCANNNNNTGG 2 cut(s) 1168, 2489
PinAI ACCGGT 1 cut(s) 130
Ple19I CGATCG 1 cut(s) 253
PleI GAGTC 5 cut(s) 1955, 2477, 2520, 3342, 3478
PpsI GAGTC 5 cut(s) 1955, 2477, 2520, 3342, 3478
Ppu21I YACGTR 1 cut(s) 1286
PscI ACATGT 1 cut(s) 2720
PsiI TTATAA 1 cut(s) 633
Psp6I CCWGG 3 cut(s) 2815, 3066, 3565
PspFI CCCAGC 1 cut(s) 958
PspGI CCWGG 3 cut(s) 2815, 3066, 3565
PspN4I GGNNCC 4 cut(s) 483, 1343, 2542, 3398
PspOMI GGGCCC 1 cut(s) 1341
PspPI GGNCC 5 cut(s) 1341, 1342, 1627, 2848, 3044
PstI CTGCAG 2 cut(s) 2182, 2831
PsuI RGATCY 6 cut(s) 481, 807, 1954, 2504, 2819, 3013
PvuI CGATCG 1 cut(s) 253
RsaI GTAC 8 cut(s) 495, 1791, 2258, 2524, 2766, 2877, 3027, 3284
RsaNI GTAC 8 cut(s) 494, 1790, 2257, 2523, 2765, 2876, 3026, 3283
RseI CAYNNNNRTG 1 cut(s) 2627
SapI GCTCTTC 4 cut(s) 500, 988, 2244, 3426
Sau96I GGNCC 5 cut(s) 1341, 1342, 1627, 2848, 3044
ScaI AGTACT 1 cut(s) 2524
SchI GAGTC 5 cut(s) 1956, 2478, 2521, 3343, 3478
ScrFI CCNGG 3 cut(s) 2817, 3068, 3567
SduI GDGCHC 3 cut(s) 862, 1345, 2483
SexAI ACCWGGT 1 cut(s) 3565
SfcI CTRYAG 3 cut(s) 2178, 2673, 2827
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 2 cut(s) 2848, 3044
SmiMI CAYNNNNRTG 1 cut(s) 2627
SmlI CTYRAG 5 cut(s) 56, 2081, 2509, 2866, 3488
SmoI CTYRAG 5 cut(s) 56, 2081, 2509, 2866, 3488
SsiI CCGC 4 cut(s) 1963, 2037, 2451, 3057
SspI AATATT 1 cut(s) 2320
SspMI CTAG 6 cut(s) 47, 177, 1296, 1592, 3017, 3449
StyD4I CCNGG 3 cut(s) 2815, 3066, 3565
StyI CCWWGG 4 cut(s) 176, 1167, 1778, 3106
TaaI ACNGT 8 cut(s) 28, 2569, 2642, 2769, 2880, 2930, 3166, 3235
TaiI ACGT 2 cut(s) 141, 1288
TatI WGTACW 4 cut(s) 1789, 2256, 2522, 2875
TauI GCSGC 3 cut(s) 1965, 2040, 2454
TscAI CASTG 9 cut(s) 581, 630, 1972, 2572, 2587, 2861, 3143, 3537, 3561
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 7 cut(s) 571, 1379, 1503, 2177, 2180, 2448, 3345
Tsp45I GTSAC 1 cut(s) 28
TspGWI ACGGA 3 cut(s) 828, 1842, 2840
TspRI CASTG 9 cut(s) 581, 630, 1972, 2572, 2587, 2861, 3143, 3537, 3561
Van91I CCANNNNNTGG 2 cut(s) 1168, 2489
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 2848, 3044
XagI CCTNNNNNAGG 1 cut(s) 3570
XapI RAATTY 9 cut(s) 456, 604, 968, 1089, 1713, 1830, 3259, 3525, 3665
XbaI TCTAGA 1 cut(s) 3016
XceI RCATGY 3 cut(s) 1591, 2263, 2724
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmaJI CCTAGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 1312
XmnI GAANNNNTTC 1 cut(s) 3270
XspI CTAG 6 cut(s) 47, 177, 1296, 1592, 3017, 3449
ZraI GACGTC 1 cut(s) 139
ZrmI AGTACT 1 cut(s) 2524
Zsp2I ATGCAT 3 cut(s) 437, 1445, 1686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.