Rorug03G0210700

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0018237
Physical Location & Seq
Forward (+)
18819529 .. 18821879
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0210700.1

Sequence Viewer

Length: 1167 bp
ATGATTCGTCGTGGGGATGATAAGATACACACCTTTCGTAACACCTTTCGTTCTTGTAGCACCTATCGTTTTCTTACCTTCTGCGCAGATGCTAACCCTAGAATCATCAATTGGATTGACAATGCATTAAGGTGTAACATTGAAGTGCTTGACCTTGAGCTCTGTATGGTTTCTTTGACTCGGCTGTTTCAGTCTATCTTGCTTTGTGGATCTTTGAAGTCTTTATCAGTGGGAATGGAGTATAATTACGTGTCCCTAACATTACCCTCCTCGGCTTGTTTCTCTAATATTGAATGCTTGAAGTTGAAAAACATTGAGTTAGTGGATGAGGGGTTTTTCAAATGGATCTCGTCTACTTGCAAATCCATTAAGAAATTACATCTTGAAGAAATTCAGGGAATAAAACATCTCAGCATTGAAAGTTCATCTTTGCAATCGTTTAGTTTTGTGTATCACTATCGCCTACAGATGGACCCCTTCCATCTTAGCATTTCTGCTGACAATCTGGAAGATCTAGTTATTAATTGGGGATTTCATGGTTGCCAAAAGTTAAATATATTTGCTCCGAAAGTTAGATATATGAAATGGACAGGGAAATTGTTGATTCACCAAAATTTTGGTGAATTTAAGCTTCTAGAACAAGCTGAGCTTTATCTGAGACCTGGAGGGAATGACTTTCTATCTGAGTTTCTTAGCAGCATATGCAGGGTCCAAGCTCTTATTATGAAGGGAGATACCTTAAATACCATAAATGCTTTGTTCGAGAAAGGTTCCATGACAGCACCTTTAGATAATGTTCGTCAACTGCAGCTTCATGTTGGGTACTTCTGTGATGACCTAGTCCCGGCCATGGTCTCTCTTTTGAAAGGATTGCCTAATTTGAGTACATTGAACATAAATATTGACCCAGTAGAATACTGCCCTAGAACTAATGAAAGCATAGAGCTTCCTAATGGGTCTAATGGAATCGAGTTAGCATGGTATATACTAGATTATGCTCAAAGTTTGAAGAAAATGGTGATTGTTCATTCACCCGAGCAATCTTATTTCGAAAGGAAGTTGCTAAAAAGAAAAAAGATTTCCAATGTCATAGTTGTCTTTCAGGAAGATCAAAGAAGAGGAATTGCAGAGCAAATTAGAAGAAGAAGGTTGTCAAATAAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

388

Amino Acids

44.63

Weight (kDa)

8.63

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 38 - 176 8.3e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018237)

Species Orthologous Gene IDs
rosa_laevigata RLG00000023193 RLG00000023203
rosa_multiflora Rmu_sc0006653.1_g000007
rosa_roxburghii Rroxscaffold_6G00397400 Rroxscaffold_6G00397420
rosa_rugosa Rorug03G0210700
rosa_samantha Rh2AG350100 Rh3BG299300 Rh3BG299700 Rh3DG292900
rosa_wichuraiana Rw3G023570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 85
AccI GTMKAC 1 cut(s) 353
AclWI GGATC 2 cut(s) 217, 353
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 3 cut(s) 390, 613, 623
AfaI GTAC 2 cut(s) 824, 886
AfiI CCNNNNNNNGG 3 cut(s) 469, 844, 850
AflIII ACRYGT 1 cut(s) 249
AjnI CCWGG 1 cut(s) 661
AjuI GAANNNNNNNTTGG 2 cut(s) 94, 126
AluBI AGCT 7 cut(s) 160, 631, 644, 649, 716, 811, 946
AluI AGCT 7 cut(s) 160, 631, 644, 649, 716, 811, 946
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 2 cut(s) 652, 859
AlwI GGATC 2 cut(s) 217, 353
Ama87I CYCGRG 1 cut(s) 1034
AoxI GGCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 696, 808
ApoI RAATTY 3 cut(s) 390, 613, 623
AseI ATTAAT 1 cut(s) 522
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 472, 709
AsuC2I CCSGG 1 cut(s) 845
AsuHPI GGTGA 4 cut(s) 599, 632, 1023, 1030
AsuII TTCGAA 1 cut(s) 1050
AvaI CYCGRG 1 cut(s) 1034
AvaII GGWCC 2 cut(s) 472, 709
BaeI ACNNNNGTAYC 2 cut(s) 814, 847
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 708, 820
BccI CCATC 2 cut(s) 463, 489
BciT130I CCWGG 1 cut(s) 663
BcnI CCSGG 1 cut(s) 845
BcoDI GTCTC 2 cut(s) 652, 859
BfaI CTAG 6 cut(s) 99, 515, 635, 839, 924, 989
BfmI CTRYAG 2 cut(s) 464, 806
BglII AGATCT 1 cut(s) 511
BisI GCNGC 2 cut(s) 697, 809
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 2 cut(s) 698, 810
Bme1390I CCNGG 2 cut(s) 663, 845
Bme18I GGWCC 2 cut(s) 472, 709
BmeT110I CYCGRG 1 cut(s) 1034
BmgT120I GGNCC 2 cut(s) 472, 709
BmiI GGNNCC 3 cut(s) 474, 710, 772
BmrFI CCNGG 2 cut(s) 663, 845
BmrI ACTGGG 1 cut(s) 902
BmsI GCATC 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 902
BpmI CTGGAG 1 cut(s) 684
Bpu1102I GCTNAGC 1 cut(s) 645
Bpu14I TTCGAA 1 cut(s) 1050
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 845
BsaAI YACGTR 1 cut(s) 250
BsaI GGTCTC 2 cut(s) 652, 859
BsaJI CCNNGG 2 cut(s) 270, 849
Bsc4I CCNNNNNNNGG 3 cut(s) 469, 844, 850
Bse1I ACTGG 1 cut(s) 908
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 2 cut(s) 270, 849
BseGI GGATG 2 cut(s) 22, 331
BseLI CCNNNNNNNGG 3 cut(s) 469, 844, 850
BseMII CTCAG 4 cut(s) 424, 636, 647, 675
BseNI ACTGG 1 cut(s) 908
BseRI GAGGAG 1 cut(s) 259
BseXI GCAGC 2 cut(s) 708, 820
BshFI GGCC 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 1034
BsiSI CCGG 1 cut(s) 845
BslFI GGGAC 2 cut(s) 238, 827
BslI CCNNNNNNNGG 3 cut(s) 469, 844, 850
BsmAI GTCTC 2 cut(s) 652, 859
BsmFI GGGAC 2 cut(s) 238, 827
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 1 cut(s) 848
Bso31I GGTCTC 2 cut(s) 652, 859
BsoBI CYCGRG 1 cut(s) 1034
Bsp119I TTCGAA 1 cut(s) 1050
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 4 cut(s) 209, 345, 511, 1108
Bsp1720I GCTNAGC 1 cut(s) 645
Bsp19I CCATGG 1 cut(s) 849
BspANI GGCC 1 cut(s) 848
BspCNI CTCAG 4 cut(s) 423, 637, 648, 676
BspLI GGNNCC 3 cut(s) 474, 710, 772
BspMAI CTGCAG 1 cut(s) 810
BspPI GGATC 2 cut(s) 217, 353
BspT104I TTCGAA 1 cut(s) 1050
BspTNI GGTCTC 2 cut(s) 652, 859
BsrI ACTGG 1 cut(s) 908
BssECI CCNNGG 2 cut(s) 270, 849
BssMI GATC 4 cut(s) 209, 345, 511, 1108
BssT1I CCWWGG 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 663
Bst6I CTCTTC 1 cut(s) 1111
BstAPI GCANNNNNTGC 1 cut(s) 702
BstBAI YACGTR 1 cut(s) 250
BstBI TTCGAA 1 cut(s) 1050
BstDEI CTNAG 6 cut(s) 410, 485, 645, 656, 684, 692
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 2 cut(s) 22, 331
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 4 cut(s) 212, 348, 514, 1111
BstMAI GTCTC 2 cut(s) 652, 859
BstMBI GATC 4 cut(s) 209, 345, 511, 1108
BstMWI GCNNNNNNNGC 1 cut(s) 702
BstNI CCWGG 1 cut(s) 663
BstSCI CCNGG 2 cut(s) 661, 843
BstSFI CTRYAG 2 cut(s) 464, 806
BstV1I GCAGC 2 cut(s) 708, 820
BstX2I RGATCY 3 cut(s) 209, 345, 511
BstXI CCANNNNNNTGG 1 cut(s) 617
BstYI RGATCY 3 cut(s) 209, 345, 511
BsuRI GGCC 1 cut(s) 848
BtgI CCRYGG 1 cut(s) 849
BtsCI GGATG 2 cut(s) 22, 331
BtsIMutI CAGTG 1 cut(s) 234
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 472, 709
Csp6I GTAC 2 cut(s) 823, 885
CviAII CATG 5 cut(s) 536, 775, 815, 850, 978
CviQI GTAC 2 cut(s) 823, 885
DdeI CTNAG 6 cut(s) 410, 485, 645, 656, 684, 692
DpnI GATC 4 cut(s) 211, 347, 513, 1110
DpnII GATC 4 cut(s) 209, 345, 511, 1108
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 1 cut(s) 1111
EarI CTCTTC 1 cut(s) 1111
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 849
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 2 cut(s) 652, 859
Eco47I GGWCC 2 cut(s) 472, 709
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 1034
EcoICRI GAGCTC 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 849
EcoT22I ATGCAT 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 849
FaeI CATG 5 cut(s) 539, 778, 818, 853, 981
FalI AAGNNNNNCTT 4 cut(s) 412, 444, 633, 665
FaqI GGGAC 2 cut(s) 238, 827
FatI CATG 5 cut(s) 535, 774, 814, 849, 977
FauNDI CATATG 1 cut(s) 701
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 2 cut(s) 697, 809
FokI GGATG 2 cut(s) 29, 338
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 2 cut(s) 697, 809
FspBI CTAG 6 cut(s) 99, 515, 635, 839, 924, 989
FspI TGCGCA 1 cut(s) 85
GlaI GCGC 1 cut(s) 85
GluI GCNGC 2 cut(s) 697, 809
GsuI CTGGAG 1 cut(s) 684
HaeIII GGCC 1 cut(s) 848
HapII CCGG 1 cut(s) 845
HhaI GCGC 1 cut(s) 86
Hin1II CATG 5 cut(s) 539, 778, 818, 853, 981
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 803
HindII GTYRAC 1 cut(s) 803
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 5 cut(s) 4, 102, 178, 604, 966
HpaII CCGG 1 cut(s) 845
HphI GGTGA 4 cut(s) 599, 632, 1023, 1030
Hpy166II GTNNAC 2 cut(s) 354, 803
Hpy188I TCNGA 3 cut(s) 567, 657, 685
Hpy188III TCNNGA 5 cut(s) 383, 506, 635, 763, 1103
Hpy8I GTNNAC 2 cut(s) 354, 803
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 4 cut(s) 88, 487, 721, 1140
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 6 cut(s) 125, 360, 433, 705, 808, 1127
HpyF10VI GCNNNNNNNGC 1 cut(s) 702
HpyF3I CTNAG 6 cut(s) 410, 485, 645, 656, 684, 692
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 5 cut(s) 539, 778, 818, 853, 981
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 4 cut(s) 209, 345, 511, 1108
LmnI GCTCC 1 cut(s) 568
LpnPI CCDG 9 cut(s) 380, 491, 576, 648, 675, 691, 858, 921, 1088
Lsp1109I GCAGC 2 cut(s) 708, 820
LweI GCATC 1 cut(s) 79
MaeI CTAG 6 cut(s) 99, 515, 635, 839, 924, 989
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 2 cut(s) 38, 134
MalI GATC 4 cut(s) 211, 347, 513, 1110
MboI GATC 4 cut(s) 209, 345, 511, 1108
MboII GAAGA 7 cut(s) 398, 521, 1021, 1118, 1128, 1152, 1155
MfeI CAATTG 1 cut(s) 109
MflI RGATCY 3 cut(s) 209, 345, 511
MhlI GDGCHC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 172
MnlI CCTC 5 cut(s) 277, 280, 322, 659, 1112
Mph1103I ATGCAT 1 cut(s) 127
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 6 cut(s) 128, 369, 522, 551, 627, 740
MslI CAYNNNNRTG 2 cut(s) 130, 143
MspI CCGG 1 cut(s) 845
MspR9I CCNGG 2 cut(s) 663, 845
MunI CAATTG 1 cut(s) 109
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 1 cut(s) 702
NciI CCSGG 1 cut(s) 845
NcoI CCATGG 1 cut(s) 849
NdeI CATATG 1 cut(s) 701
NdeII GATC 4 cut(s) 209, 345, 511, 1108
NlaIII CATG 5 cut(s) 539, 778, 818, 853, 981
NlaIV GGNNCC 3 cut(s) 474, 710, 772
NmeAIII GCCGAG 2 cut(s) 160, 251
NsbI TGCGCA 1 cut(s) 85
NsiI ATGCAT 1 cut(s) 127
NspV TTCGAA 1 cut(s) 1050
PctI GAATGC 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 4 cut(s) 4, 102, 604, 966
PflFI GACNNNGTC 1 cut(s) 839
PkrI GCNGC 2 cut(s) 698, 810
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 250
PshBI ATTAAT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PspN4I GGNNCC 3 cut(s) 474, 710, 772
PspPI GGNCC 2 cut(s) 472, 709
PstI CTGCAG 1 cut(s) 810
PsuI RGATCY 3 cut(s) 209, 345, 511
PsyI GACNNNGTC 1 cut(s) 839
RsaI GTAC 2 cut(s) 824, 886
RsaNI GTAC 2 cut(s) 823, 885
RseI CAYNNNNRTG 2 cut(s) 130, 143
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 6 cut(s) 128, 369, 522, 551, 627, 740
SatI GCNGC 2 cut(s) 697, 809
Sau3AI GATC 4 cut(s) 209, 345, 511, 1108
Sau96I GGNCC 2 cut(s) 472, 709
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 663, 845
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 2 cut(s) 464, 806
SfuI TTCGAA 1 cut(s) 1050
SinI GGWCC 2 cut(s) 472, 709
SmiMI CAYNNNNRTG 2 cut(s) 130, 143
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SspI AATATT 2 cut(s) 289, 901
SspMI CTAG 6 cut(s) 99, 515, 635, 839, 924, 989
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 661, 843
StyI CCWWGG 1 cut(s) 849
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 762, 969, 1050
TatI WGTACW 1 cut(s) 884
TfiI GAWTC 4 cut(s) 4, 102, 604, 966
Tru1I TTAA 6 cut(s) 128, 369, 522, 551, 627, 740
Tru9I TTAA 6 cut(s) 128, 369, 522, 551, 627, 740
TscAI CASTG 1 cut(s) 234
TseI GCWGC 2 cut(s) 696, 808
TspDTI ATGAA 7 cut(s) 414, 524, 596, 740, 803, 948, 1016
TspRI CASTG 1 cut(s) 234
Tth111I GACNNNGTC 1 cut(s) 839
VpaK11BI GGWCC 2 cut(s) 472, 709
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 3 cut(s) 390, 613, 623
XbaI TCTAGA 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 6 cut(s) 99, 515, 635, 839, 924, 989
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.