Rorug03G0219500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010306
Physical Location & Seq
Forward (+)
20057390 .. 20058610
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0219500.1

Sequence Viewer

Length: 429 bp
ATGGCTGCTTCTGTGACTGGAACAGCACATGAAGATGCTGCAATTAGAGGGGGGGATAAGCTTGTACTAAGGGGTTTGAAGTTCCATGGTTTCCATGGGGTGAAGCCAGAAGAAAGGAAGTTGGGTCAGAAGTTTCTGATAGATGTTGATGCTTGGATGGATCTTCGGGTGGCCGGAAGATCTGATCAGTTGTCAGACACCATTAGCTACACTGAAATTTATCGCATTGTAAAAGAGGTTGTAGAAGGGCCACCTCATAATCTTCTGGAGTCTGTTGCTGAACTCATTGCATCTACCACTCTGAAAAACTATCCCCAGATATCTGCTGTCTCTGTGAAAGTTGGAAAGCCTCACGTTGCTGTTCATGGTCCTCTTGATTACTTGGGCGTTGAGATATTTAGATACAGAAGTATTGATGCACCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

15.61

Weight (kDa)

6.59

Isoelectric Point (pI)

20.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FolB PF02152 22 - 132 8.4e-37 Dihydroneopterin aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 216
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 1 cut(s) 79
AluBI AGCT 2 cut(s) 61, 207
AluI AGCT 2 cut(s) 61, 207
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 1 cut(s) 168
AoxI GGCC 2 cut(s) 171, 248
ApeKI GCWGC 2 cut(s) 5, 38
ApoI RAATTY 1 cut(s) 216
AspS9I GGNCC 2 cut(s) 248, 368
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 368
BaeI ACNNNNGTAYC 2 cut(s) 394, 427
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 151
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 334
BglII AGATCT 1 cut(s) 179
BisI GCNGC 2 cut(s) 6, 39
BlsI GCNGC 2 cut(s) 7, 40
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 248, 368
BmsI GCATC 4 cut(s) 25, 139, 299, 406
BpmI CTGGAG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 85, 94
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 85, 94
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 25
BshFI GGCC 2 cut(s) 173, 250
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 334
BsnI GGCC 2 cut(s) 173, 250
Bsp143I GATC 3 cut(s) 160, 179, 184
Bsp19I CCATGG 2 cut(s) 85, 94
BspANI GGCC 2 cut(s) 173, 250
BspPI GGATC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 85, 94
BssMI GATC 3 cut(s) 160, 179, 184
BssT1I CCWWGG 2 cut(s) 85, 94
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 2 cut(s) 85, 94
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 3 cut(s) 163, 182, 187
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 3 cut(s) 160, 179, 184
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 160, 179
BstYI RGATCY 2 cut(s) 160, 179
BsuRI GGCC 2 cut(s) 173, 250
BtgI CCRYGG 2 cut(s) 85, 94
BtsCI GGATG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 248, 368
Csp6I GTAC 1 cut(s) 65
CviAII CATG 4 cut(s) 29, 86, 95, 365
CviJI RGCY 7 cut(s) 5, 61, 106, 173, 207, 250, 349
CviKI_1 RGCY 7 cut(s) 5, 61, 106, 173, 207, 250, 349
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 68
DpnI GATC 3 cut(s) 162, 181, 186
DpnII GATC 3 cut(s) 160, 179, 184
EaeI YGGCCR 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 85, 94
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 368
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 85, 94
ErhI CCWWGG 2 cut(s) 85, 94
FaeI CATG 4 cut(s) 32, 89, 98, 368
FaiI YATR 5 cut(s) 30, 87, 96, 258, 366
FatI CATG 4 cut(s) 28, 85, 94, 364
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 2 cut(s) 6, 39
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 6, 39
GluI GCNGC 2 cut(s) 6, 39
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 173, 250
HapII CCGG 1 cut(s) 174
Hin1II CATG 4 cut(s) 32, 89, 98, 368
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 112
Hpy188I TCNGA 5 cut(s) 129, 138, 184, 196, 303
Hpy188III TCNNGA 2 cut(s) 266, 374
HpyAV CCTTC 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 3 cut(s) 41, 290, 419
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 4 cut(s) 32, 89, 98, 368
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 160, 179, 184
LpnPI CCDG 5 cut(s) 3, 120, 187, 251, 329
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 4 cut(s) 25, 139, 299, 406
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 1 cut(s) 13
MalI GATC 3 cut(s) 162, 181, 186
MboI GATC 3 cut(s) 160, 179, 184
MboII GAAGA 5 cut(s) 44, 122, 155, 189, 254
MflI RGATCY 2 cut(s) 160, 179
MluCI AATT 2 cut(s) 42, 216
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 5 cut(s) 41, 229, 264, 360, 381
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 1 cut(s) 174
NcoI CCATGG 2 cut(s) 85, 94
NdeII GATC 3 cut(s) 160, 179, 184
NlaIII CATG 4 cut(s) 32, 89, 98, 368
NmuCI GTSAC 1 cut(s) 13
PkrI GCNGC 2 cut(s) 7, 40
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PspPI GGNCC 2 cut(s) 248, 368
PsuI RGATCY 2 cut(s) 160, 179
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 33
SatI GCNGC 2 cut(s) 6, 39
Sau3AI GATC 3 cut(s) 160, 179, 184
Sau96I GGNCC 2 cut(s) 248, 368
SchI GAGTC 1 cut(s) 278
SetI ASST 5 cut(s) 63, 209, 240, 256, 357
SfaNI GCATC 4 cut(s) 25, 139, 299, 406
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 2 cut(s) 42, 216
StyI CCWWGG 2 cut(s) 85, 94
TaiI ACGT 1 cut(s) 357
TasI AATT 2 cut(s) 42, 216
TatI WGTACW 1 cut(s) 64
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 2 cut(s) 5, 38
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 45, 353
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 216
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.