Rorug03G0219700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010306
Physical Location & Seq
Forward (+)
20069335 .. 20069784
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0219700.1

Sequence Viewer

Length: 450 bp
ATGAGCGTGGACATTCTCAGTTGGAACTGCAGAGGGATATGTAATCCAGCCACAACTCGAGCCTTACGGGATCTGGTCAGCCAGAGTCGACCTCAGATCGTGTTTTTGTGTGAAACAAAAATTAGTCAATTACCAGATTTCCAGGCCCTTCATAGGGATCTAGGGTTTGCTCATTTTAAAGAGGTGTTGAGCGAAGGCCAGGCCGGTGGCCTCGGAGTGTTCTGGAAGGAAGATGTTGGTGTTCAGATACGCACGGTTTCGGCTCATCATATTGATATGGAAGTGTGTACAGGTCTAGGAGAACCACGCTGGCGTCTTACTGGGTTCTATGGCTTTGCACGTACGTGTGAACGTGATCTTTCTTGGCAGCTACTTAGGGATTTATGTGACCTAGATTCCCTGCATTGGGTGATTATTGGGGATTTTAACGAGATTCTGACTAATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.88

Weight (kDa)

5.45

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 145 1.6e-13 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 88
AclWI GGATC 2 cut(s) 78, 165
AcyI GRCGYC 1 cut(s) 313
AfaI GTAC 2 cut(s) 289, 343
AfiI CCNNNNNNNGG 4 cut(s) 153, 154, 405, 406
AflIII ACRYGT 1 cut(s) 344
AjnI CCWGG 2 cut(s) 141, 198
AleI CACNNNNGTG 1 cut(s) 343
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
AlwI GGATC 2 cut(s) 78, 165
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 4 cut(s) 144, 196, 201, 208
ApeKI GCWGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 57
BbvI GCAGC 1 cut(s) 379
BciT130I CCWGG 2 cut(s) 143, 200
BfaI CTAG 3 cut(s) 161, 296, 392
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 2 cut(s) 143, 200
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 145
BmrFI CCNGG 2 cut(s) 143, 200
BmrI ACTGGG 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 76, 108
BsaAI YACGTR 2 cut(s) 341, 345
BsaHI GRCGYC 1 cut(s) 313
BsaJI CCNNGG 1 cut(s) 211
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 154, 405, 406
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 2 cut(s) 143, 200
BseDI CCNNGG 1 cut(s) 211
BseLI CCNNNNNNNGG 4 cut(s) 153, 154, 405, 406
BseMII CTCAG 2 cut(s) 31, 107
BseNI ACTGG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 4 cut(s) 146, 198, 203, 210
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 204
BsiWI CGTACG 1 cut(s) 341
BslI CCNNNNNNNGG 4 cut(s) 153, 154, 405, 406
BsnI GGCC 4 cut(s) 146, 198, 203, 210
BsoBI CYCGRG 1 cut(s) 57
Bsp1407I TGTACA 1 cut(s) 287
Bsp143I GATC 4 cut(s) 70, 96, 157, 355
BspANI GGCC 4 cut(s) 146, 198, 203, 210
BspCNI CTCAG 2 cut(s) 30, 106
BspMAI CTGCAG 1 cut(s) 32
BspPI GGATC 2 cut(s) 78, 165
BsrFI RCCGGY 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 287
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 4 cut(s) 70, 96, 157, 355
BssNI GRCGYC 1 cut(s) 313
Bst2UI CCWGG 2 cut(s) 143, 200
Bst4CI ACNGT 1 cut(s) 256
BstACI GRCGYC 1 cut(s) 313
BstAUI TGTACA 1 cut(s) 287
BstBAI YACGTR 2 cut(s) 341, 345
BstC8I GCNNGC 1 cut(s) 311
BstDEI CTNAG 3 cut(s) 17, 93, 374
BstKTI GATC 4 cut(s) 73, 99, 160, 358
BstMBI GATC 4 cut(s) 70, 96, 157, 355
BstNI CCWGG 2 cut(s) 143, 200
BstSCI CCNGG 2 cut(s) 141, 198
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 70, 157
BstXI CCANNNNNNTGG 1 cut(s) 206
BstYI RGATCY 2 cut(s) 70, 157
BsuRI GGCC 4 cut(s) 146, 198, 203, 210
Cac8I GCNNGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 145
CseI GACGC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 288, 342
CviQI GTAC 2 cut(s) 288, 342
DdeI CTNAG 3 cut(s) 17, 93, 374
DpnI GATC 4 cut(s) 72, 98, 159, 357
DpnII GATC 4 cut(s) 70, 96, 157, 355
DraI TTTAAA 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 141, 198
FaiI YATR 6 cut(s) 40, 153, 270, 278, 330, 385
FblI GTMKAC 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 3 cut(s) 161, 296, 392
GluI GCNGC 1 cut(s) 368
HaeIII GGCC 4 cut(s) 146, 198, 203, 210
HapII CCGG 1 cut(s) 204
HgaI GACGC 1 cut(s) 302
Hin1I GRCGYC 1 cut(s) 313
HincII GTYRAC 1 cut(s) 89
HindII GTYRAC 1 cut(s) 89
HinfI GANTC 3 cut(s) 85, 395, 433
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 4 cut(s) 10, 89, 288, 350
Hpy188I TCNGA 4 cut(s) 96, 215, 246, 438
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 4 cut(s) 10, 89, 288, 350
HpyAV CCTTC 3 cut(s) 158, 188, 220
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 3 cut(s) 340, 344, 352
HpyCH4V TGCA 3 cut(s) 30, 338, 403
HpyF3I CTNAG 3 cut(s) 17, 93, 374
HpySE526I ACGT 3 cut(s) 340, 344, 352
Hsp92I GRCGYC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 70, 96, 157, 355
Lsp1109I GCAGC 1 cut(s) 379
MaeI CTAG 3 cut(s) 161, 296, 392
MaeII ACGT 3 cut(s) 340, 344, 352
MaeIII GTNAC 1 cut(s) 386
MalI GATC 4 cut(s) 72, 98, 159, 357
MboI GATC 4 cut(s) 70, 96, 157, 355
MboII GAAGA 1 cut(s) 242
MflI RGATCY 2 cut(s) 70, 157
MluCI AATT 2 cut(s) 120, 128
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 4 cut(s) 26, 102, 175, 221
MseI TTAA 2 cut(s) 177, 426
MslI CAYNNNNRTG 1 cut(s) 343
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 2 cut(s) 143, 200
MvaI CCWGG 2 cut(s) 143, 200
NdeII GATC 4 cut(s) 70, 96, 157, 355
NmuCI GTSAC 1 cut(s) 386
OliI CACNNNNGTG 1 cut(s) 343
PaeR7I CTCGAG 1 cut(s) 57
PfeI GAWTC 2 cut(s) 395, 433
Pfl23II CGTACG 1 cut(s) 341
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
Ppu21I YACGTR 2 cut(s) 341, 345
Psp6I CCWGG 2 cut(s) 141, 198
PspGI CCWGG 2 cut(s) 141, 198
PspLI CGTACG 1 cut(s) 341
PspPI GGNCC 1 cut(s) 145
PspXI VCTCGAGB 1 cut(s) 57
PstI CTGCAG 1 cut(s) 32
PsuI RGATCY 2 cut(s) 70, 157
RsaI GTAC 2 cut(s) 289, 343
RsaNI GTAC 2 cut(s) 288, 342
RseI CAYNNNNRTG 1 cut(s) 343
SalI GTCGAC 1 cut(s) 87
SaqAI TTAA 2 cut(s) 177, 426
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 4 cut(s) 70, 96, 157, 355
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 2 cut(s) 143, 200
SetI ASST 8 cut(s) 94, 186, 295, 343, 347, 355, 372, 393
SfcI CTRYAG 1 cut(s) 28
Sfr274I CTCGAG 1 cut(s) 57
SlaI CTCGAG 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 343
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 2 cut(s) 120, 128
SspMI CTAG 3 cut(s) 161, 296, 392
StyD4I CCNGG 2 cut(s) 141, 198
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 3 cut(s) 343, 347, 355
TaqI TCGA 2 cut(s) 58, 88
TasI AATT 2 cut(s) 120, 128
TatI WGTACW 1 cut(s) 287
TfiI GAWTC 2 cut(s) 395, 433
Tru1I TTAA 2 cut(s) 177, 426
Tru9I TTAA 2 cut(s) 177, 426
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 88
XspI CTAG 3 cut(s) 161, 296, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.