Rorug03G0319200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008463
Physical Location & Seq
Forward (+)
34472110 .. 34473539
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0319200.1

Sequence Viewer

Length: 813 bp
ATGGAAGAGATGCGTTCTTATGACCTTCAAGTGTTGCAAAGAAACCGAGGCTTGGGGGGGGACATGCTTCTCATCAGACTAAAGTTGTTCAAGGCAACAGCCAGCCAATTTAACAGCAGTTCTTATGAAGAATGTCATCAAGTAGCTGTAAGACGTTATGGGATCAGTTTTTCAGATATGAAGCGGTATTTATCATCATCATCATGGAATGAAAACGGGTTTACACGAAAAATATCTGAAACTCTTACAAGCATGGGTGCCCCTCATGATTTACTGCCGGCTATTTTTCAAACATTGTCTCAGGTGGTTGAGGCGGCCGTCTCTGCCCACCATATTGATGTGGGTGTTTGCGAAACCACTTACCTCGGGGACGGGGTGGGCGCGGGCGCAGGCGGAGGCGGTGGAGATGTTGGAGATGTTGGAGGTGGAGATGGAGATGTTGGAGGTGTAGTTGGAGATGTTGGAGGTGCTGTTCGAGCTGGAGATGGAGATGTTGGAGGTGTAGGTGGAGATGTTGGAGGTGCTGTTGGAGTTAGAGGCGGAGGTGTAGGTGGAATGATTCCTGCGATTAAATCATCAATTGATGGTTTGGAGCAAGTGACAGTTGATATTACTATTATCACACATTCACCCTCGTGTTCTATTTGTTTGGAGGATTTTGCAGCTTTTGTAGATTATCAACAAGAACCGATTACTCGGTTGCCCTGCGGACATCATTATCATGTACATTGCATTGTGCAGTGGCTGGAGTTCAGCCACCTATGTCCTCTCTGCCGATACCAAATGCCAACTCAAGATGATCAGTCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

28.44

Weight (kDa)

4.89

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 212 - 259 1.2e-12 Ring finger domain
zf-rbx1 PF12678 213 - 259 3.8e-08 RING-H2 zinc finger domain
zf-RING_UBOX PF13445 213 - 256 3.5e-07 RING-type zinc-finger
zf-RING_11 PF17123 213 - 245 2.3e-06 RING-like zinc finger
zf-C3HC4 PF00097 213 - 258 3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 257
AccII CGCG 1 cut(s) 383
AciI CCGC 7 cut(s) 184, 314, 383, 393, 399, 540, 708
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 1 cut(s) 315
AfaI GTAC 1 cut(s) 726
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 3 cut(s) 29, 91, 290
AleI CACNNNNGTG 1 cut(s) 634
AloI GAACNNNNNNTCC 2 cut(s) 456, 488
AluBI AGCT 3 cut(s) 146, 479, 665
AluI AGCT 3 cut(s) 146, 479, 665
Alw26I GTCTC 2 cut(s) 303, 325
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 745
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 662
AspLEI GCGC 2 cut(s) 383, 389
AsuHPI GGTGA 1 cut(s) 621
AvaI CYCGRG 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 262
BanI GGYRCC 1 cut(s) 257
BauI CACGAG 1 cut(s) 634
BbvI GCAGC 1 cut(s) 674
BccI CCATC 3 cut(s) 425, 479, 578
BceAI ACGGC 1 cut(s) 302
BclI TGATCA 1 cut(s) 799
BcoDI GTCTC 2 cut(s) 303, 325
BisI GCNGC 2 cut(s) 315, 663
BlsI GCNGC 2 cut(s) 316, 664
BmeT110I CYCGRG 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 259
BpmI CTGGAG 2 cut(s) 501, 767
BpuEI CTTGAG 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 46, 364
BsaXI ACNNNNNCTCC 6 cut(s) 435, 456, 465, 486, 510, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 727
BseDI CCNNGG 2 cut(s) 46, 364
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 262
BseX3I CGGCCG 1 cut(s) 315
BseXI GCAGC 1 cut(s) 674
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 383
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 1 cut(s) 317
BshNI GGYRCC 1 cut(s) 257
BsiEI CGRYCG 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 74, 383
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 2 cut(s) 303, 325
BsmBI CGTCTC 1 cut(s) 325
BsmFI GGGAC 2 cut(s) 74, 383
BsnI GGCC 1 cut(s) 317
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 262
Bsp1407I TGTACA 1 cut(s) 724
Bsp143I GATC 2 cut(s) 162, 799
BspACI CCGC 7 cut(s) 184, 314, 383, 393, 399, 540, 708
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 383
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 724
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 46, 364
BssMI GATC 2 cut(s) 162, 799
BssSI CACGAG 1 cut(s) 634
Bst2BI CACGAG 1 cut(s) 634
Bst4CI ACNGT 1 cut(s) 604
BstAUI TGTACA 1 cut(s) 724
BstC8I GCNNGC 4 cut(s) 103, 279, 385, 391
BstDEI CTNAG 1 cut(s) 300
BstFNI CGCG 1 cut(s) 383
BstHHI GCGC 2 cut(s) 383, 389
BstKTI GATC 2 cut(s) 165, 802
BstMAI GTCTC 2 cut(s) 303, 325
BstMBI GATC 2 cut(s) 162, 799
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 2 cut(s) 323, 476
BstNSI RCATGY 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 262
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 674
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 1 cut(s) 317
BtsI GCAGTG 1 cut(s) 746
BtsIMutI CAGTG 1 cut(s) 746
Cac8I GCNNGC 4 cut(s) 103, 279, 385, 391
CaiI CAGNNNCTG 1 cut(s) 745
CciI TCATGA 1 cut(s) 265
CfoI GCGC 2 cut(s) 383, 389
Cfr10I RCCGGY 1 cut(s) 277
Csp6I GTAC 1 cut(s) 725
CviAII CATG 5 cut(s) 64, 204, 253, 266, 722
CviQI GTAC 1 cut(s) 725
DdeI CTNAG 1 cut(s) 300
DpnI GATC 2 cut(s) 164, 801
DpnII GATC 2 cut(s) 162, 799
EaeI YGGCCR 1 cut(s) 315
EagI CGGCCG 1 cut(s) 315
EciI GGCGGA 2 cut(s) 408, 555
EclXI CGGCCG 1 cut(s) 315
Eco52I CGGCCG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 365
Esp3I CGTCTC 1 cut(s) 325
FaeI CATG 5 cut(s) 67, 207, 256, 269, 725
FaqI GGGAC 2 cut(s) 74, 383
FatI CATG 5 cut(s) 63, 203, 252, 265, 721
FauI CCCGC 1 cut(s) 376
FbaI TGATCA 1 cut(s) 799
Fnu4HI GCNGC 2 cut(s) 315, 663
Fsp4HI GCNGC 2 cut(s) 315, 663
GlaI GCGC 2 cut(s) 382, 388
GluI GCNGC 2 cut(s) 315, 663
GsuI CTGGAG 2 cut(s) 501, 767
HaeIII GGCC 1 cut(s) 317
HapII CCGG 1 cut(s) 278
HhaI GCGC 2 cut(s) 383, 389
Hin1II CATG 5 cut(s) 67, 207, 256, 269, 725
Hin6I GCGC 2 cut(s) 381, 387
HinP1I GCGC 2 cut(s) 381, 387
HinfI GANTC 1 cut(s) 559
HpaII CCGG 1 cut(s) 278
HphI GGTGA 1 cut(s) 621
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 3 cut(s) 77, 175, 238
Hpy188III TCNNGA 2 cut(s) 266, 794
Hpy8I GTNNAC 1 cut(s) 222
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 604
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 4 cut(s) 37, 662, 732, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 476
HpyF3I CTNAG 1 cut(s) 300
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 5 cut(s) 67, 207, 256, 269, 725
HspAI GCGC 2 cut(s) 381, 387
KroI GCCGGC 1 cut(s) 277
KroNI GCCGGC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 2 cut(s) 162, 799
LmnI GCTCC 1 cut(s) 592
LpnPI CCDG 8 cut(s) 115, 287, 291, 375, 465, 576, 718, 731
Lsp1109I GCAGC 1 cut(s) 674
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 1 cut(s) 598
MalI GATC 2 cut(s) 164, 801
MboI GATC 2 cut(s) 162, 799
MboII GAAGA 2 cut(s) 17, 140
MfeI CAATTG 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 3 cut(s) 107, 579, 808
MmeI TCCRAC 8 cut(s) 391, 400, 421, 433, 442, 475, 496, 508
MroNI GCCGGC 1 cut(s) 277
MseI TTAA 2 cut(s) 111, 570
MslI CAYNNNNRTG 4 cut(s) 202, 336, 634, 720
MspI CCGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 579
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 323, 476
NaeI GCCGGC 1 cut(s) 279
NdeII GATC 2 cut(s) 162, 799
NgoMIV GCCGGC 1 cut(s) 277
NlaIII CATG 5 cut(s) 67, 207, 256, 269, 725
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 598
NspI RCATGY 1 cut(s) 67
OliI CACNNNNGTG 1 cut(s) 634
PagI TCATGA 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 559
PkrI GCNGC 2 cut(s) 316, 664
PspN4I GGNNCC 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 745
RsaI GTAC 1 cut(s) 726
RsaNI GTAC 1 cut(s) 725
RseI CAYNNNNRTG 4 cut(s) 202, 336, 634, 720
SaqAI TTAA 2 cut(s) 111, 570
SatI GCNGC 2 cut(s) 315, 663
Sau3AI GATC 2 cut(s) 162, 799
SduI GDGCHC 1 cut(s) 262
SmiMI CAYNNNNRTG 4 cut(s) 202, 336, 634, 720
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
Sse9I AATT 3 cut(s) 107, 579, 808
SsiI CCGC 7 cut(s) 184, 314, 383, 393, 399, 540, 708
TaaI ACNGT 1 cut(s) 604
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 475
TasI AATT 3 cut(s) 107, 579, 808
TatI WGTACW 1 cut(s) 724
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 1 cut(s) 559
Tru1I TTAA 2 cut(s) 111, 570
Tru9I TTAA 2 cut(s) 111, 570
TscAI CASTG 1 cut(s) 746
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 3 cut(s) 141, 194, 225
TspRI CASTG 1 cut(s) 746
XceI RCATGY 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.