Rorug04G0048500

tRNA (adenine-N6-)-methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014478
Physical Location & Seq
Reverse (-)
7489689 .. 7490816
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0048500.1

Sequence Viewer

Length: 1128 bp
ATGCCTTCCAGTTACTTGCTTCTGTTTCTTCTTGGAGTGACGGTTTTCACTATCACTCCTTCTCTTGCTGATCACCGCGATGATCACCACAAGCCACCGATTCAAAAGCCTCCTACCCATCCCTCAGACAACCACAAACCGCCCATCCACAAACCTCCCATGGTGGAAGTCTCTCACCTTCAGAAGTCACCACAACCCCCCAAACACAAGCCTCCAACAACATTAACTAAGCAAGAGCCACTCCCGGAGCACAAGCCATCAACCCCACGTGATCAACCTCCAAAGGGTAAGGGAGAGAAGCCACCACCGGAGCACAAGTCACCTCATGATCACCGCTTATTGGAGACCGATGGAAAATCTTCTATGGTAGACAAGTCACCACGAGAGCACAATGAGCCTCGTAAGGGAGAAAAGCCACCACCGGAGCACAAAGGAAACCCAGGCGAGCAACCTCATCACAAGGGTCACGAGCACAAACCCCCCCATGAACAAAACGTCGGACGTCGTTTGTTAGAGTCTTCTTCCTCAGAGTTGGATGGTAAGCCACCTAAGGGAGAGAAGCCACCACAAGAACGCAGTGAGCCTCACAAGGAAGTTAAGCCACCGCCAAAGCATAAGCCACCTCATGAACACCAGTTGTTAGAGTTGGATGGAAAACCTTCCAAGGGAGAGAAGCCATCGCAAGAGCACAATGAGCCTCGTAAGGGAGATAAGCCACCACCAGAGCACAAACCAGGACATCCAGGTAATGAACTTCATAAGGGTGAGAAGCCACCACCAAACCACAAACCACCTCATGAACAAAACACTGGACGCCATTTGCTAGAGTCTTCCTCAACTGAGTTGGATGGTAAGTCTCCTAAAGGAAAGAAGCCACCCCCAAAACACAAGCCGCCAACCCCACTTGACAAAAATGATGATCACAAGCCATTCCCGGAACAGAAGCCACCAAAGGGTAAGGGAGAGAAGCCACCATATCACGACCGTCACCTTGGAGAGAATGCAGAGAAGGATGCCAGAGACCCACCACACAAGCCACCTCGGAAATTAAAGCCTCCAAGTACTGTTGCAAAGAAGCCTCCAACTGCCCCTCACAAGCCACCTCACAAACCTCCATCTCCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroa_b-bind

Protein Analysis

375

Amino Acids

42.04

Weight (kDa)

9.57

Isoelectric Point (pI)

58.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014478)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28025 AT4G28025
fragaria_vesca FvH4_4g08570 FvH4_4g08570
malus_domestica MD16G1263600.v1.1
prunus_persica Prupe.1G095600_v2.0.a1
pyrus_communis pycom16g23250
rosa_chinensis RchiOBHm_Chr4g0404651
rosa_laevigata RLG00000008916
rosa_multiflora Rmu_sc0002699.1_g000039
rosa_roxburghii Rroxscaffold_5G00348800
rosa_rugosa Rorug04G0048500
rosa_samantha Rh4AG124200 Rh4BG118300 Rh4CG132300 Rh4DG117300
rosa_wichuraiana Rw4G010070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 369
AccII CGCG 1 cut(s) 78
AciI CCGC 5 cut(s) 76, 140, 334, 605, 893
AcuI CTGAAG 1 cut(s) 164
AcvI CACGTG 1 cut(s) 269
AcyI GRCGYC 2 cut(s) 502, 814
AfaI GTAC 1 cut(s) 1063
AfiI CCNNNNNNNGG 7 cut(s) 284, 340, 404, 551, 665, 704, 953
AgsI TTSAA 1 cut(s) 104
AjnI CCWGG 3 cut(s) 439, 733, 742
Alw21I GWGCWC 7 cut(s) 252, 315, 390, 429, 474, 690, 729
Alw26I GTCTC 4 cut(s) 175, 338, 861, 1014
Asp700I GAANNNNTTC 2 cut(s) 358, 658
AsuC2I CCSGG 2 cut(s) 245, 935
AsuHPI GGTGA 9 cut(s) 65, 77, 167, 180, 312, 323, 369, 776, 980
AxyI CCTNAGG 1 cut(s) 549
BauI CACGAG 2 cut(s) 381, 467
BbrPI CACGTG 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 510, 822
Bbv12I GWGCWC 7 cut(s) 252, 315, 390, 429, 474, 690, 729
BccI CCATC 9 cut(s) 126, 152, 265, 344, 530, 644, 685, 842, 1123
BciT130I CCWGG 3 cut(s) 441, 735, 744
BclI TGATCA 5 cut(s) 70, 82, 271, 328, 919
BcnI CCSGG 2 cut(s) 245, 935
BcoDI GTCTC 4 cut(s) 175, 338, 861, 1014
BfaI CTAG 1 cut(s) 824
BisI GCNGC 1 cut(s) 893
BlsI GCNGC 1 cut(s) 894
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 5 cut(s) 245, 441, 735, 744, 935
BmrFI CCNGG 5 cut(s) 245, 441, 735, 744, 935
BmsI GCATC 1 cut(s) 1003
BpiI GAAGAC 2 cut(s) 510, 822
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpuMI CCSGG 2 cut(s) 245, 935
BsaAI YACGTR 1 cut(s) 269
BsaHI GRCGYC 2 cut(s) 502, 814
BsaI GGTCTC 2 cut(s) 338, 1014
BsaJI CCNNGG 5 cut(s) 159, 439, 663, 991, 1040
BsaWI WCCGGW 2 cut(s) 307, 421
BsaXI ACNNNNNCTCC 6 cut(s) 27, 40, 57, 70, 302, 332
Bsc4I CCNNNNNNNGG 7 cut(s) 284, 340, 404, 551, 665, 704, 953
Bse1I ACTGG 3 cut(s) 9, 634, 814
Bse21I CCTNAGG 1 cut(s) 549
BseBI CCWGG 3 cut(s) 441, 735, 744
BseDI CCNNGG 5 cut(s) 159, 439, 663, 991, 1040
BseGI GGATG 7 cut(s) 118, 144, 541, 655, 739, 853, 1018
BseLI CCNNNNNNNGG 7 cut(s) 284, 340, 404, 551, 665, 704, 953
BseMII CTCAG 3 cut(s) 138, 540, 831
BseNI ACTGG 3 cut(s) 9, 634, 814
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 985
BsiEI CGRYCG 1 cut(s) 985
BsiHKAI GWGCWC 7 cut(s) 252, 315, 390, 429, 474, 690, 729
BsiSI CCGG 4 cut(s) 245, 308, 422, 935
BslI CCNNNNNNNGG 7 cut(s) 284, 340, 404, 551, 665, 704, 953
BsmAI GTCTC 4 cut(s) 175, 338, 861, 1014
BsmI GAATGC 1 cut(s) 1006
Bso31I GGTCTC 2 cut(s) 338, 1014
Bsp1286I GDGCHC 7 cut(s) 252, 315, 390, 429, 474, 690, 729
Bsp143I GATC 5 cut(s) 70, 82, 271, 328, 919
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 5 cut(s) 76, 140, 334, 605, 893
BspCNI CTCAG 3 cut(s) 137, 539, 832
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 3 cut(s) 325, 625, 796
BspTNI GGTCTC 2 cut(s) 338, 1014
BsrI ACTGG 3 cut(s) 9, 634, 814
BssECI CCNNGG 5 cut(s) 159, 439, 663, 991, 1040
BssMI GATC 5 cut(s) 70, 82, 271, 328, 919
BssNI GRCGYC 2 cut(s) 502, 814
BssSI CACGAG 2 cut(s) 381, 467
BssT1I CCWWGG 3 cut(s) 159, 663, 991
Bst2BI CACGAG 2 cut(s) 381, 467
Bst2UI CCWGG 3 cut(s) 441, 735, 744
Bst4CI ACNGT 3 cut(s) 43, 986, 1066
BstACI GRCGYC 2 cut(s) 502, 814
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 5 cut(s) 124, 228, 526, 549, 840
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 7 cut(s) 118, 144, 541, 655, 739, 853, 1018
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 5 cut(s) 73, 85, 274, 331, 922
BstMAI GTCTC 4 cut(s) 175, 338, 861, 1014
BstMBI GATC 5 cut(s) 70, 82, 271, 328, 919
BstMCI CGRYCG 1 cut(s) 985
BstMWI GCNNNNNNNGC 2 cut(s) 394, 694
BstNI CCWGG 3 cut(s) 441, 735, 744
BstSCI CCNGG 5 cut(s) 243, 439, 733, 742, 933
BstUI CGCG 1 cut(s) 78
BstV2I GAAGAC 2 cut(s) 510, 822
Bsu36I CCTNAGG 1 cut(s) 549
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 2 cut(s) 93, 663
BtsCI GGATG 7 cut(s) 118, 144, 541, 655, 739, 853, 1018
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 2 cut(s) 583, 807
Cac8I GCNNGC 1 cut(s) 446
CciI TCATGA 3 cut(s) 325, 625, 796
CseI GACGC 1 cut(s) 822
Csp6I GTAC 1 cut(s) 1062
CviAII CATG 5 cut(s) 160, 326, 485, 626, 797
CviQI GTAC 1 cut(s) 1062
DdeI CTNAG 5 cut(s) 124, 228, 526, 549, 840
DpnI GATC 5 cut(s) 72, 84, 273, 330, 921
DpnII GATC 5 cut(s) 70, 82, 271, 328, 919
Eco130I CCWWGG 3 cut(s) 159, 663, 991
Eco31I GGTCTC 2 cut(s) 338, 1014
Eco57I CTGAAG 1 cut(s) 164
Eco72I CACGTG 1 cut(s) 269
Eco81I CCTNAGG 1 cut(s) 549
EcoRII CCWGG 3 cut(s) 439, 733, 742
EcoT14I CCWWGG 3 cut(s) 159, 663, 991
ErhI CCWWGG 3 cut(s) 159, 663, 991
FaeI CATG 5 cut(s) 163, 329, 488, 629, 800
FaiI YATR 9 cut(s) 161, 327, 365, 486, 615, 627, 759, 798, 976
FatI CATG 5 cut(s) 159, 325, 484, 625, 796
FbaI TGATCA 5 cut(s) 70, 82, 271, 328, 919
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 1 cut(s) 893
FokI GGATG 7 cut(s) 105, 131, 548, 662, 726, 860, 1025
Fsp4HI GCNGC 1 cut(s) 893
FspBI CTAG 1 cut(s) 824
GluI GCNGC 1 cut(s) 893
HapII CCGG 4 cut(s) 245, 308, 422, 935
HgaI GACGC 1 cut(s) 822
Hin1I GRCGYC 2 cut(s) 502, 814
Hin1II CATG 5 cut(s) 163, 329, 488, 629, 800
HinfI GANTC 3 cut(s) 100, 515, 827
HpaII CCGG 4 cut(s) 245, 308, 422, 935
HphI GGTGA 9 cut(s) 65, 77, 167, 180, 312, 323, 369, 776, 980
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 5 cut(s) 127, 183, 500, 529, 1044
Hpy188III TCNNGA 5 cut(s) 326, 467, 626, 797, 980
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 2 cut(s) 500, 507
HpyAV CCTTC 5 cut(s) 15, 69, 188, 669, 1003
HpyCH4III ACNGT 3 cut(s) 43, 986, 1066
HpyCH4IV ACGT 3 cut(s) 268, 495, 502
HpyCH4V TGCA 2 cut(s) 1004, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 694
HpyF3I CTNAG 5 cut(s) 124, 228, 526, 549, 840
HpySE526I ACGT 3 cut(s) 268, 495, 502
Hsp92I GRCGYC 2 cut(s) 502, 814
Hsp92II CATG 5 cut(s) 163, 329, 488, 629, 800
Ksp22I TGATCA 5 cut(s) 70, 82, 271, 328, 919
Kzo9I GATC 5 cut(s) 70, 82, 271, 328, 919
LmnI GCTCC 3 cut(s) 247, 310, 424
LweI GCATC 1 cut(s) 1003
MaeI CTAG 1 cut(s) 824
MaeII ACGT 3 cut(s) 268, 495, 502
MaeIII GTNAC 7 cut(s) 11, 37, 186, 318, 375, 464, 986
MalI GATC 5 cut(s) 72, 84, 273, 330, 921
MboI GATC 5 cut(s) 70, 82, 271, 328, 919
MboII GAAGA 5 cut(s) 20, 351, 510, 513, 822
MhlI GDGCHC 7 cut(s) 252, 315, 390, 429, 474, 690, 729
MluCI AATT 1 cut(s) 1046
MlyI GAGTC 2 cut(s) 524, 836
MmeI TCCRAC 6 cut(s) 239, 478, 513, 627, 825, 1106
MroXI GAANNNNTTC 2 cut(s) 358, 658
MseI TTAA 3 cut(s) 224, 597, 1049
MslI CAYNNNNRTG 2 cut(s) 78, 762
MspI CCGG 4 cut(s) 245, 308, 422, 935
MspR9I CCNGG 5 cut(s) 245, 441, 735, 744, 935
Mva1269I GAATGC 1 cut(s) 1006
MvaI CCWGG 3 cut(s) 441, 735, 744
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 394, 694
NciI CCSGG 2 cut(s) 245, 935
NcoI CCATGG 1 cut(s) 159
NdeII GATC 5 cut(s) 70, 82, 271, 328, 919
NlaIII CATG 5 cut(s) 163, 329, 488, 629, 800
NmuCI GTSAC 6 cut(s) 37, 186, 318, 375, 464, 986
PagI TCATGA 3 cut(s) 325, 625, 796
PctI GAATGC 1 cut(s) 1006
PdmI GAANNNNTTC 2 cut(s) 358, 658
PfeI GAWTC 1 cut(s) 100
PfoI TCCNGGA 2 cut(s) 243, 933
PkrI GCNGC 1 cut(s) 894
PleI GAGTC 2 cut(s) 523, 835
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 2 cut(s) 523, 835
Ppu21I YACGTR 1 cut(s) 269
Psp6I CCWGG 3 cut(s) 439, 733, 742
PspCI CACGTG 1 cut(s) 269
PspGI CCWGG 3 cut(s) 439, 733, 742
RsaI GTAC 1 cut(s) 1063
RsaNI GTAC 1 cut(s) 1062
RseI CAYNNNNRTG 2 cut(s) 78, 762
SaqAI TTAA 3 cut(s) 224, 597, 1049
SatI GCNGC 1 cut(s) 893
Sau3AI GATC 5 cut(s) 70, 82, 271, 328, 919
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 2 cut(s) 524, 836
ScrFI CCNGG 5 cut(s) 245, 441, 735, 744, 935
SduI GDGCHC 7 cut(s) 252, 315, 390, 429, 474, 690, 729
SfaNI GCATC 1 cut(s) 1003
SmiMI CAYNNNNRTG 2 cut(s) 78, 762
Sse9I AATT 1 cut(s) 1046
SsiI CCGC 5 cut(s) 76, 140, 334, 605, 893
SspMI CTAG 1 cut(s) 824
StyD4I CCNGG 5 cut(s) 243, 439, 733, 742, 933
StyI CCWWGG 3 cut(s) 159, 663, 991
TaaI ACNGT 3 cut(s) 43, 986, 1066
TaiI ACGT 3 cut(s) 271, 498, 505
TaqII GACCGA 1 cut(s) 362
TasI AATT 1 cut(s) 1046
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 1 cut(s) 895
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 3 cut(s) 224, 597, 1049
Tru9I TTAA 3 cut(s) 224, 597, 1049
TscAI CASTG 2 cut(s) 583, 814
TseFI GTSAC 6 cut(s) 37, 186, 318, 375, 464, 986
Tsp45I GTSAC 6 cut(s) 37, 186, 318, 375, 464, 986
TspDTI ATGAA 5 cut(s) 501, 642, 746, 765, 813
TspRI CASTG 2 cut(s) 583, 814
XmiI GTMKAC 1 cut(s) 369
XmnI GAANNNNTTC 2 cut(s) 358, 658
XspI CTAG 1 cut(s) 824
ZraI GACGTC 1 cut(s) 503
ZrmI AGTACT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.