Rorug04G0066500

Integral membrane protein TerC family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017863
Physical Location & Seq
Forward (+)
10233176 .. 10281182
48007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0066500.1

Sequence Viewer

Length: 1206 bp
ATGGCTTACTCCACTCTTGCTTCACCCTCCACCTCCTTCATCAACAACACCACCAGGGATTTGGGTCTCTCTGCTTTCTCTTCCACGTCGCCGGCTTCATTTCCGAGATGCAAGAGATCGGTCTCCAATAAAATCTGCTCCGTCATGGCGCCACAGCAATCGGAGCGGAAGCCAGCCACTACTGGCTCGGTCAAGACGGGAATGACGATGACGGAGAAGATATTGGCTAGGGCTTCTGAGAAACCCCAATTGAGCCCAGGTGACAATGTTTGGGTTAACATTGATGTTTTGATGACCCATGACGTGTGTGGCCCTGGTTCATTTGGAATCTTCAAGAAAGAGTTTGGACAGAATGCAAAGGTTTGGGACCGAGAAAAGATTGTAATCATACCTGACCATTATATATTTACTACTGATGAGCGTGCAAACCGTAATGTGGATATCTTGAGGGATTTCTGCACGGAGCAGAACATCAAGTACTTCTATGATATCAAGGATCTTGGTAACTTTAAGGCTAACCCAGATTACAAGGGTGTATGCCATGTTGCTCTTGCTCAAGAAGGTCATTGCAGGCCTGGAGAGGTGCTGCTAGGTACAGATTCTCACACCTGCACCGCTGGAGCTTTTGGACAATTTGCTACTGGAATCGGGAATACTGATGCAGGTTTCGTCTTGGGCACTGGGAAGCTTTTGCTCAAGGTGCCTCCAACTCTGAGATTTGTGCTGGATGGTGAAATGCCTGACTATTTGCTTGCCAAAGACTTGATTTTACAAATTATTGGTGAAATCTCTGTTGCTGGTGCAACATATAAAGCTATGGAGTTTGTTGGCAGCACTGTTGAAGCCTTAAGTATGGAAGAGAGGATGACATTATGCAACATGGTTGTTGAAGCTGGGGGTAAAAACGGTATCGTCCCTGCTGATAGCACTACATACAAGTACCTTGAGGATAAAACGTCTGTACCCTATGAACCTGTGTACAGTGATGATACAGCTAGATTTCTTACAGAGTACAGATTTGATATCTCTAAACTGGAGCCATTGGTGGCAAAGCCTCATTCTCCAGATAACCGTGCTTTAGCAAGAGAGTGCAAAGATGTCAAAATTGACAGAGTCTATATTGGATCTTGTACTGGTGGGAAAACAGAGGATTTTATGGCTGCTGCTAAAGTTTTTCTAGCTTCAGTAAGGACTCCAATAACTTAG

Protein Analysis

401

Amino Acids

43.89

Weight (kDa)

6.13

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 89 - 359 4.5e-56 Aconitase family (aconitate hydratase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 2 cut(s) 617, 653
AccB1I GGYRCC 2 cut(s) 148, 700
AccBSI CCGCTC 1 cut(s) 166
AciI CCGC 2 cut(s) 166, 615
AclWI GGATC 2 cut(s) 504, 1132
AcuI CTGAAG 1 cut(s) 1167
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 7 cut(s) 479, 595, 941, 963, 980, 1013, 1132
AfiI CCNNNNNNNGG 1 cut(s) 436
AflII CTTAAG 1 cut(s) 847
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 3 cut(s) 334, 842, 890
AjiI CACGTC 2 cut(s) 87, 304
AjnI CCWGG 4 cut(s) 53, 256, 313, 574
AluBI AGCT 6 cut(s) 623, 688, 815, 893, 995, 1181
AluI AGCT 6 cut(s) 623, 688, 815, 893, 995, 1181
Alw26I GTCTC 2 cut(s) 71, 127
AlwI GGATC 2 cut(s) 504, 1132
AoxI GGCC 2 cut(s) 310, 572
ApeKI GCWGC 4 cut(s) 586, 831, 1160, 1163
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 2 cut(s) 311, 367
AsuHPI GGTGA 4 cut(s) 15, 272, 743, 794
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 680
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BanI GGYRCC 2 cut(s) 148, 700
BanII GRGCYC 1 cut(s) 257
BbvI GCAGC 4 cut(s) 573, 843, 1147, 1150
BccI CCATC 1 cut(s) 722
BciT130I CCWGG 4 cut(s) 55, 258, 315, 576
BcoDI GTCTC 2 cut(s) 71, 127
BfaI CTAG 4 cut(s) 228, 590, 996, 1178
BfoI RGCGCY 1 cut(s) 152
BfrI CTTAAG 1 cut(s) 847
BfuAI ACCTGC 2 cut(s) 617, 653
BisI GCNGC 4 cut(s) 587, 832, 1161, 1164
BlsI GCNGC 4 cut(s) 588, 833, 1162, 1165
BmcAI AGTACT 1 cut(s) 479
Bme1390I CCNGG 4 cut(s) 55, 258, 315, 576
Bme18I GGWCC 1 cut(s) 367
BmgBI CACGTC 2 cut(s) 87, 304
BmgT120I GGNCC 2 cut(s) 311, 367
BmiI GGNNCC 4 cut(s) 150, 368, 702, 1038
BmrFI CCNGG 4 cut(s) 55, 258, 315, 576
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 2 cut(s) 98, 649
BmuI ACTGGG 1 cut(s) 690
BpmI CTGGAG 4 cut(s) 597, 639, 1047, 1055
BpuEI CTTGAG 4 cut(s) 466, 540, 680, 965
BsaBI GATNNNNATC 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 2 cut(s) 71, 127
BsaJI CCNNGG 3 cut(s) 54, 256, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 5 cut(s) 187, 646, 685, 1038, 1138
Bse3DI GCAATG 1 cut(s) 565
Bse8I GATNNNNATC 1 cut(s) 383
BseBI CCWGG 4 cut(s) 55, 258, 315, 576
BseDI CCNNGG 3 cut(s) 54, 256, 313
BseGI GGATG 2 cut(s) 733, 870
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 565
BseMII CTCAG 2 cut(s) 228, 704
BseNI ACTGG 5 cut(s) 187, 646, 685, 1038, 1138
BseSI GKGCMC 1 cut(s) 680
BseXI GCAGC 4 cut(s) 573, 843, 1147, 1150
BseYI CCCAGC 1 cut(s) 893
BsgI GTGCAG 2 cut(s) 442, 595
BshFI GGCC 2 cut(s) 312, 574
BshNI GGYRCC 2 cut(s) 148, 700
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 2 cut(s) 380, 899
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 2 cut(s) 71, 127
BsmFI GGGAC 2 cut(s) 380, 899
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 2 cut(s) 312, 574
Bso31I GGTCTC 2 cut(s) 71, 127
Bsp1286I GDGCHC 2 cut(s) 257, 680
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 3 cut(s) 116, 496, 1124
BspACI CCGC 2 cut(s) 166, 615
BspANI GGCC 2 cut(s) 312, 574
BspCNI CTCAG 2 cut(s) 229, 705
BspLI GGNNCC 4 cut(s) 150, 368, 702, 1038
BspMI ACCTGC 2 cut(s) 617, 653
BspPI GGATC 2 cut(s) 504, 1132
BspT107I GGYRCC 2 cut(s) 148, 700
BspTI CTTAAG 1 cut(s) 847
BspTNI GGTCTC 2 cut(s) 71, 127
BsrBI CCGCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 565
BsrFI RCCGGY 1 cut(s) 91
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 5 cut(s) 187, 646, 685, 1038, 1138
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 54, 256, 313
BssMI GATC 3 cut(s) 116, 496, 1124
BssNI GRCGYC 1 cut(s) 149
Bst2UI CCWGG 4 cut(s) 55, 258, 315, 576
Bst4CI ACNGT 5 cut(s) 431, 838, 908, 983, 1073
Bst6I CTCTTC 2 cut(s) 85, 852
BstACI GRCGYC 1 cut(s) 149
BstAFI CTTAAG 1 cut(s) 847
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 5 cut(s) 93, 174, 423, 572, 753
BstDEI CTNAG 3 cut(s) 237, 713, 1203
BstF5I GGATG 2 cut(s) 733, 870
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 3 cut(s) 119, 499, 1127
BstMAI GTCTC 2 cut(s) 71, 127
BstMBI GATC 3 cut(s) 116, 496, 1124
BstMWI GCNNNNNNNGC 2 cut(s) 163, 700
BstNI CCWGG 4 cut(s) 55, 258, 315, 576
BstSCI CCNGG 4 cut(s) 53, 256, 313, 574
BstSLI GKGCMC 1 cut(s) 680
BstV1I GCAGC 4 cut(s) 573, 843, 1147, 1150
BstX2I RGATCY 2 cut(s) 496, 1124
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 2 cut(s) 496, 1124
BsuRI GGCC 2 cut(s) 312, 574
BtrI CACGTC 2 cut(s) 87, 304
BtsCI GGATG 2 cut(s) 733, 870
BtsIMutI CAGTG 3 cut(s) 678, 834, 988
BveI ACCTGC 2 cut(s) 617, 653
Cac8I GCNNGC 5 cut(s) 93, 174, 423, 572, 753
CfoI GCGC 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 311, 367
Csp6I GTAC 7 cut(s) 478, 594, 940, 962, 979, 1012, 1131
CviAII CATG 4 cut(s) 145, 299, 542, 880
CviQI GTAC 7 cut(s) 478, 594, 940, 962, 979, 1012, 1131
DdeI CTNAG 3 cut(s) 237, 713, 1203
DinI GGCGCC 1 cut(s) 150
DpnI GATC 3 cut(s) 118, 498, 1126
DpnII GATC 3 cut(s) 116, 496, 1124
Eam1104I CTCTTC 2 cut(s) 85, 852
EarI CTCTTC 2 cut(s) 85, 852
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 257
Eco31I GGTCTC 2 cut(s) 71, 127
Eco32I GATATC 3 cut(s) 442, 490, 1024
Eco47I GGWCC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 1167
EcoRII CCWGG 4 cut(s) 53, 256, 313, 574
EcoRV GATATC 3 cut(s) 442, 490, 1024
EcoT38I GRGCYC 1 cut(s) 257
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
FaeI CATG 4 cut(s) 148, 302, 545, 883
FaqI GGGAC 2 cut(s) 380, 899
FatI CATG 4 cut(s) 144, 298, 541, 879
Fnu4HI GCNGC 4 cut(s) 587, 832, 1161, 1164
FokI GGATG 2 cut(s) 740, 877
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 4 cut(s) 587, 832, 1161, 1164
FspBI CTAG 4 cut(s) 228, 590, 996, 1178
GlaI GCGC 1 cut(s) 150
GluI GCNGC 4 cut(s) 587, 832, 1161, 1164
GsaI CCCAGC 1 cut(s) 897
GsuI CTGGAG 4 cut(s) 597, 639, 1047, 1055
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 2 cut(s) 312, 574
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 4 cut(s) 148, 302, 545, 883
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 5 cut(s) 327, 599, 645, 1113, 1192
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 92
HphI GGTGA 4 cut(s) 15, 272, 743, 794
Hpy166II GTNNAC 2 cut(s) 277, 979
Hpy188I TCNGA 4 cut(s) 105, 163, 238, 714
Hpy188III TCNNGA 6 cut(s) 193, 334, 445, 557, 649, 1064
Hpy8I GTNNAC 2 cut(s) 277, 979
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 46, 554
HpyCH4III ACNGT 5 cut(s) 431, 838, 908, 983, 1073
HpyCH4IV ACGT 3 cut(s) 86, 303, 956
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 700
HpyF3I CTNAG 3 cut(s) 237, 713, 1203
HpySE526I ACGT 3 cut(s) 86, 303, 956
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 4 cut(s) 148, 302, 545, 883
HspAI GCGC 1 cut(s) 149
KasI GGCGCC 1 cut(s) 148
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 116, 496, 1124
LmnI GCTCC 5 cut(s) 143, 163, 463, 620, 1036
Lsp1109I GCAGC 4 cut(s) 573, 843, 1147, 1150
LweI GCATC 2 cut(s) 98, 649
MaeI CTAG 4 cut(s) 228, 590, 996, 1178
MaeII ACGT 3 cut(s) 86, 303, 956
MaeIII GTNAC 2 cut(s) 260, 503
MalI GATC 3 cut(s) 118, 498, 1126
MbiI CCGCTC 1 cut(s) 166
MboI GATC 3 cut(s) 116, 496, 1124
MboII GAAGA 4 cut(s) 72, 229, 322, 869
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 2 cut(s) 496, 1124
MhlI GDGCHC 2 cut(s) 257, 680
MluCI AATT 4 cut(s) 248, 632, 774, 1104
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 2 cut(s) 1122, 1186
MmeI TCCRAC 1 cut(s) 731
MnlI CCTC 9 cut(s) 37, 43, 441, 574, 714, 855, 940, 1065, 1141
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 3 cut(s) 276, 510, 848
MspA1I CMGCKG 1 cut(s) 617
MspCI CTTAAG 1 cut(s) 847
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 4 cut(s) 55, 258, 315, 576
MunI CAATTG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 4 cut(s) 55, 258, 315, 576
MwoI GCNNNNNNNGC 2 cut(s) 163, 700
NaeI GCCGGC 1 cut(s) 93
NarI GGCGCC 1 cut(s) 149
NdeII GATC 3 cut(s) 116, 496, 1124
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 4 cut(s) 148, 302, 545, 883
NlaIV GGNNCC 4 cut(s) 150, 368, 702, 1038
NmuCI GTSAC 1 cut(s) 260
PaqCI CACCTGC 1 cut(s) 617
PceI AGGCCT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 1 cut(s) 358
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 327, 599, 645
PflFI GACNNNGTC 1 cut(s) 1112
PkrI GCNGC 4 cut(s) 588, 833, 1162, 1165
PleI GAGTC 2 cut(s) 1121, 1186
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 2 cut(s) 1121, 1186
Psp6I CCWGG 4 cut(s) 53, 256, 313, 574
PspFI CCCAGC 1 cut(s) 893
PspGI CCWGG 4 cut(s) 53, 256, 313, 574
PspN4I GGNNCC 4 cut(s) 150, 368, 702, 1038
PspPI GGNCC 2 cut(s) 311, 367
PsrI GAACNNNNNNTAC 2 cut(s) 461, 493
PsuI RGATCY 2 cut(s) 496, 1124
PsyI GACNNNGTC 1 cut(s) 1112
RsaI GTAC 7 cut(s) 479, 595, 941, 963, 980, 1013, 1132
RsaNI GTAC 7 cut(s) 478, 594, 940, 962, 979, 1012, 1131
SaqAI TTAA 3 cut(s) 276, 510, 848
SatI GCNGC 4 cut(s) 587, 832, 1161, 1164
Sau3AI GATC 3 cut(s) 116, 496, 1124
Sau96I GGNCC 2 cut(s) 311, 367
ScaI AGTACT 1 cut(s) 479
SchI GAGTC 2 cut(s) 1122, 1186
ScrFI CCNGG 4 cut(s) 55, 258, 315, 576
SduI GDGCHC 2 cut(s) 257, 680
SfaNI GCATC 2 cut(s) 98, 649
SfoI GGCGCC 1 cut(s) 150
SinI GGWCC 1 cut(s) 367
SmlI CTYRAG 5 cut(s) 445, 555, 695, 847, 944
SmoI CTYRAG 5 cut(s) 445, 555, 695, 847, 944
Sse9I AATT 4 cut(s) 248, 632, 774, 1104
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 2 cut(s) 166, 615
SspDI GGCGCC 1 cut(s) 148
SspMI CTAG 4 cut(s) 228, 590, 996, 1178
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 4 cut(s) 53, 256, 313, 574
TaaI ACNGT 5 cut(s) 431, 838, 908, 983, 1073
TaiI ACGT 3 cut(s) 89, 306, 959
TaqII GACCGA 3 cut(s) 109, 178, 384
TasI AATT 4 cut(s) 248, 632, 774, 1104
TatI WGTACW 4 cut(s) 477, 978, 1011, 1130
TfiI GAWTC 3 cut(s) 327, 599, 645
Tru1I TTAA 3 cut(s) 276, 510, 848
Tru9I TTAA 3 cut(s) 276, 510, 848
TscAI CASTG 3 cut(s) 685, 841, 988
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 4 cut(s) 586, 831, 1160, 1163
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 4 cut(s) 28, 87, 309, 984
TspGWI ACGGA 3 cut(s) 130, 227, 476
TspRI CASTG 3 cut(s) 685, 841, 988
Tth111I GACNNNGTC 1 cut(s) 1112
Vha464I CTTAAG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 367
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 305
XspI CTAG 4 cut(s) 228, 590, 996, 1178
ZrmI AGTACT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.