Rorug04G0206600

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008816
Physical Location & Seq
Forward (+)
35959308 .. 35965266
5959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0206600.1

Sequence Viewer

Length: 2397 bp
ATGGGAGACCTCGCCGTTACTTCCAAGGAGGGCCCTAAGCTCCCCATCCCCGGCCAACGCAACGTCCTCATCACCAGCGCGCTGCCCTACGTCAACAACGTCCCTCATCTCGGCAACATCATCGGCTGCGTTCTCAGCGCCGACGTCTTCGCTCGCTACTGCCGTCTCCGGGGATACAACACCGTCTATATCTGCGGCACCGATGAGTACGGCACCGCCACCGAGACCAAAGCCATGGAGGAGAAGTGTAGCCCCAAGGAGATCTGCGATAAGTACTATGCGATTCATCGGCAGGTGTATGAGTGGTTCAATATTAGTTTTGACAAATTCGGGCGAACTTCGACGCCGGAGCAGACGGAGGTGTGCCAAGCGATTTTCAATAGGCTGCTGGAGAATAATTGGCTTTCTGAGAACACAATGCAGCAGCTATATTGTGATACTTGCACACGGTTTTTGGCCGACAGGCTCGTGGAGGGAACGTGCCCAACGCAAGGTTGTGATTATACTTCTGCAAGAGGAGATCAGTGTGAGAACTGTGGGAAGCTTTTAAACCCCACAGAATTAAAGGATCCAAAGTGCAAGGTCTGTAAGTCGACTCCACGGATTCGTGATACAAACCATTTGTTTCTGGAACTGCCTTTGCTGAAGGATAGGTTGGAAGAGTATATTAATAAAATGTCAGTGGAGGGGTCTTGGAGCCAGAATGCCATTCATTCAACAAATGCGTGGCTTAGAGAGGGGCTTAAACAACGGTGTATCACCAGGGATCTGAAGTGGGGGGTTCCAGTTCCACATGATAACTTCAGGGACAAGGTTTTCTATGTGTGGTTTGATGCGCCTATTGGATATGTTTCAATTACTAAATGCTACACTCCAGATTGGGAGAAATGGTGGAAGAATCCAGAGGATGTGGAGTTGTATCAGTTTATGGGCAAAGATAATGTGCCATTCCACACGGTGATGTTTCCTTCCACACTTCTTGGAACTGGTGAAAATTGGACCTTAATGAAGACAATTAGTGTTACTGAATACTTGAACTATGAAGCAGGAAAGTTTTCCAAGAGTAAAGGGGTTGGAGTATTTGGAAACGATGCCAAAGATACAAACATTCCTGTTGAAGTATGGCGATATTACTTGCTAACCAATAGGCCCGAGGTGTCGGATACATTATTTGCATGGGCGGATTTGCAAGCGAAGCTCAACAGTGAATTGCTGAATAATTTGGGCAACTTCATTAACCGAGTTTTGAGTTTTGTTGCCAAACCTTTAGGTCAAGGATATGACTCCATTATTCCAGATGCTCAAGATGCCATCTCTGATCCTTTGACAGAGAAAATAGCTGAAAAAGTGGGTAAATGTGTGGAGCAATATGTAGAGGCCATGGAGAAGGTAAAACTAAAGCAAGGACTCAAACTAGCAATGAGCATTTCAAGTGAAGGGAATGCATATCTGCAAGAAAGTCAGTTCTGGAAGCTTTACAAGGAGGATCAAACTCGCTGCTCCATTGTTATGAGAACTGCAGTGGGGCTAGTGCATCTTCTTGCTTGCTTATTAGAACCCTTCATGCCATCTTTTTCTGTTGAGGTATTTAAGCAGCTTAATTTGTCTCCTGAGACGCACATATCACTTTGTGATGATAAAGGTGATATTGATAGGGCAAGACGGCCATGGGAGATTGTACCTAGAGGTCACAAGATTGGGAAACCAGAGCCGTTGTTCAAGGAATTGAATGATGATGAAGTAGATTCTCTAAGGAAGAGGTTTGCTGGAAGTCAGGCCGATAGAAAGGAAAGGGAAGAAGCTGAAGCAGTGAAGTTGGCTGAGCAGCTCAAGAAATCAAAAGTTTCAGACAAGAGTGGAAAGAAGAAACAACAAGCCACAAAATCTGCAACTGAAGCAGAGATTTCCATATCTAGACTAGATATTCGTGTCGGCCTCATTACAAAAGCTCAGAAGCATCCTGATGCTGATGCACTTTATGTGGAAGAGATTGATGTTGGCGAAGGACAGCCTAGAACAGTTGTCAGTGGACTAGTCAAGTTTATACCTCTTGAAGAAATGCAGAATCGGAAGGTATGCGTCCTTTGCAACCTGAAGCCAGCGACCATGCGGGGTATTAAATCACAAGCAATGGTTCTTGCTGCTTCAAATAGTGACCACACCGCGGTTGAGTTGGTTGATCCACCAAAATCTGCTCAAGTTGGGGAGAGAGTTACATTTCCGGGGTTCGAAGGTGAGCCTGATGATGTGTTGAACCCCAAGAAGAAGGTTTGGGAAACCCTACAGGTAGATTTGCACAGTAACACTGAGCTAGTCGCATGCTACAAAGATATTCCCCTCACTACATCAGCTGGTGTTTGCACTGTTAAATCTATTCGTGGTGGGTCAATTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 WW

Protein Analysis

798

Amino Acids

90.07

Weight (kDa)

6.27

Isoelectric Point (pI)

26.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1g PF09334 22 - 416 2.3e-154 tRNA synthetases class I (M)
tRNA-synt_1 PF00133 24 - 265 1.1e-08 tRNA synthetases class I (I, L, M and V)
Anticodon_3 PF19303 443 - 592 2.4e-20 Anticodon binding domain of methionyl tRNA ligase
tRNA_bind PF01588 642 - 737 6.1e-33 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 283
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 283
AccB1I GGYRCC 2 cut(s) 197, 212
AccI GTMKAC 1 cut(s) 593
AccII CGCG 2 cut(s) 80, 2165
AciI CCGC 6 cut(s) 195, 216, 1181, 2110, 2163, 2165
AclWI GGATC 6 cut(s) 563, 576, 774, 1313, 1494, 2174
AcoI YGGCCR 3 cut(s) 52, 456, 1664
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 6 cut(s) 665, 787, 791, 1824, 1914, 2114
AcyI GRCGYC 2 cut(s) 144, 344
AdeI CACNNNGTG 2 cut(s) 958, 1631
AfaI GTAC 3 cut(s) 209, 275, 1680
AfiI CCNNNNNNNGG 5 cut(s) 50, 110, 169, 491, 2164
AhlI ACTAGT 1 cut(s) 2032
AjnI CCWGG 1 cut(s) 761
AjuI GAANNNNNNNTTGG 2 cut(s) 638, 670
Alw26I GTCTC 4 cut(s) 170, 218, 1607, 1611
AlwI GGATC 6 cut(s) 563, 576, 774, 1313, 1494, 2174
Ama87I CYCGRG 1 cut(s) 1151
AoxI GGCC 8 cut(s) 31, 52, 456, 1148, 1377, 1664, 1776, 1933
ApaI GGGCCC 1 cut(s) 35
ApeKI GCWGC 9 cut(s) 82, 126, 385, 421, 424, 1497, 1594, 1825, 2141
ApoI RAATTY 1 cut(s) 326
ArsI GACNNNNNNTTYG 2 cut(s) 1908, 1940
AseI ATTAAT 1 cut(s) 669
Asp700I GAANNNNTTC 1 cut(s) 1054
AspLEI GCGC 4 cut(s) 80, 82, 140, 838
AspS9I GGNCC 4 cut(s) 31, 32, 999, 1149
AsuC2I CCSGG 3 cut(s) 51, 170, 2223
AsuHPI GGTGA 6 cut(s) 64, 751, 970, 1001, 1655, 2246
AsuII TTCGAA 1 cut(s) 2229
AvaI CYCGRG 1 cut(s) 1151
AvaII GGWCC 1 cut(s) 999
BaeGI GKGCMC 2 cut(s) 35, 485
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 2 cut(s) 197, 212
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 1 cut(s) 467
BbsI GAAGAC 2 cut(s) 139, 1016
BbvI GCAGC 9 cut(s) 69, 113, 372, 433, 436, 1484, 1606, 1837, 2128
BccI CCATC 3 cut(s) 53, 1319, 1576
BceAI ACGGC 4 cut(s) 147, 226, 1679, 1696
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 1 cut(s) 763
BciVI GTATCC 2 cut(s) 167, 1156
BcnI CCSGG 3 cut(s) 51, 170, 2223
BcoDI GTCTC 4 cut(s) 170, 218, 1607, 1611
BcuI ACTAGT 1 cut(s) 2032
BfaI CTAG 8 cut(s) 1415, 1529, 1683, 1914, 1919, 2013, 2033, 2312
BfmI CTRYAG 2 cut(s) 1518, 2282
BfoI RGCGCY 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 2 cut(s) 167, 1156
BglII AGATCT 1 cut(s) 261
BlpI GCTNAGC 1 cut(s) 1821
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 4 cut(s) 51, 170, 763, 2223
Bme18I GGWCC 1 cut(s) 999
BmeT110I CYCGRG 1 cut(s) 1151
BmgT120I GGNCC 4 cut(s) 31, 32, 999, 1149
BmiI GGNNCC 6 cut(s) 33, 199, 214, 570, 698, 783
BmrFI CCNGG 4 cut(s) 51, 170, 763, 2223
BmsI GCATC 8 cut(s) 823, 1081, 1288, 1297, 1543, 1954, 1960, 1966
BpiI GAAGAC 2 cut(s) 139, 1016
BpmI CTGGAG 2 cut(s) 410, 858
Bpu10I CCTNAGC 1 cut(s) 36
Bpu1102I GCTNAGC 1 cut(s) 1821
Bpu14I TTCGAA 1 cut(s) 2229
BpuEI CTTGAG 3 cut(s) 1287, 1814, 2181
BpuMI CCSGG 3 cut(s) 51, 170, 2223
BsaHI GRCGYC 2 cut(s) 144, 344
BsaI GGTCTC 1 cut(s) 218
BsaXI ACNNNNNCTCC 6 cut(s) 230, 260, 464, 494, 875, 905
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 110, 169, 491, 2164
Bse1I ACTGG 2 cut(s) 785, 991
Bse3DI GCAATG 2 cut(s) 1425, 2136
BseBI CCWGG 1 cut(s) 763
BseGI GGATG 3 cut(s) 45, 913, 1957
BseLI CCNNNNNNNGG 5 cut(s) 50, 110, 169, 491, 2164
BseMI GCAATG 2 cut(s) 1425, 2136
BseMII CTCAG 6 cut(s) 148, 399, 1602, 1812, 1964, 2298
BseNI ACTGG 2 cut(s) 785, 991
BsePI GCGCGC 1 cut(s) 78
BseRI GAGGAG 2 cut(s) 254, 531
BseSI GKGCMC 2 cut(s) 35, 485
BseXI GCAGC 9 cut(s) 69, 113, 372, 433, 436, 1484, 1606, 1837, 2128
Bsh1236I CGCG 2 cut(s) 80, 2165
BshFI GGCC 8 cut(s) 33, 54, 458, 1150, 1379, 1666, 1778, 1935
BshNI GGYRCC 2 cut(s) 197, 212
BsiHKCI CYCGRG 1 cut(s) 1151
BsiSI CCGG 4 cut(s) 51, 169, 347, 2222
BslFI GGGAC 2 cut(s) 86, 821
BslI CCNNNNNNNGG 5 cut(s) 50, 110, 169, 491, 2164
BsmAI GTCTC 4 cut(s) 170, 218, 1607, 1611
BsmBI CGTCTC 2 cut(s) 170, 1607
BsmFI GGGAC 2 cut(s) 86, 821
BsmI GAATGC 2 cut(s) 709, 1447
BsnI GGCC 8 cut(s) 33, 54, 458, 1150, 1379, 1666, 1778, 1935
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 1151
Bsp119I TTCGAA 1 cut(s) 2229
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 35, 485
Bsp143I GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
Bsp1720I GCTNAGC 1 cut(s) 1821
Bsp19I CCATGG 3 cut(s) 234, 1380, 1667
BspACI CCGC 6 cut(s) 195, 216, 1181, 2110, 2163, 2165
BspANI GGCC 8 cut(s) 33, 54, 458, 1150, 1379, 1666, 1778, 1935
BspCNI CTCAG 6 cut(s) 147, 400, 1603, 1813, 1963, 2299
BspFNI CGCG 2 cut(s) 80, 2165
BspLI GGNNCC 6 cut(s) 33, 199, 214, 570, 698, 783
BspMAI CTGCAG 1 cut(s) 1522
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 6 cut(s) 563, 576, 774, 1313, 1494, 2174
BspT104I TTCGAA 1 cut(s) 2229
BspT107I GGYRCC 2 cut(s) 197, 212
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 2 cut(s) 1425, 2136
BsrI ACTGG 2 cut(s) 785, 991
BssHII GCGCGC 1 cut(s) 78
BssMI GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
BssNI GRCGYC 2 cut(s) 144, 344
BssSI CACGAG 1 cut(s) 467
BssT1I CCWWGG 5 cut(s) 24, 234, 255, 1380, 1667
Bst2BI CACGAG 1 cut(s) 467
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 9 cut(s) 184, 450, 536, 753, 958, 1205, 2020, 2300, 2365
Bst6I CTCTTC 3 cut(s) 654, 1751, 1980
BstACI GRCGYC 2 cut(s) 144, 344
BstBI TTCGAA 1 cut(s) 2229
BstC8I GCNNGC 6 cut(s) 80, 154, 1191, 1546, 2100, 2320
BstDEI CTNAG 9 cut(s) 36, 134, 408, 731, 1611, 1751, 1821, 1950, 2307
BstDSI CCRYGG 5 cut(s) 234, 599, 1380, 1667, 2163
BstF5I GGATG 3 cut(s) 45, 913, 1957
BstFNI CGCG 2 cut(s) 80, 2165
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 4 cut(s) 80, 82, 140, 838
BstKTI GATC 7 cut(s) 264, 523, 571, 769, 1321, 1489, 2182
BstMAI GTCTC 4 cut(s) 170, 218, 1607, 1611
BstMBI GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
BstMWI GCNNNNNNNGC 5 cut(s) 135, 1195, 1307, 1895, 2085
BstNI CCWGG 1 cut(s) 763
BstNSI RCATGY 1 cut(s) 2322
BstSCI CCNGG 4 cut(s) 49, 168, 761, 2221
BstSFI CTRYAG 2 cut(s) 1518, 2282
BstSLI GKGCMC 2 cut(s) 35, 485
BstUI CGCG 2 cut(s) 80, 2165
BstV1I GCAGC 9 cut(s) 69, 113, 372, 433, 436, 1484, 1606, 1837, 2128
BstV2I GAAGAC 2 cut(s) 139, 1016
BstX2I RGATCY 3 cut(s) 261, 568, 766
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 3 cut(s) 261, 568, 766
BsuI GTATCC 2 cut(s) 167, 1156
BsuRI GGCC 8 cut(s) 33, 54, 458, 1150, 1379, 1666, 1778, 1935
BtgI CCRYGG 5 cut(s) 234, 599, 1380, 1667, 2163
BtsCI GGATG 3 cut(s) 45, 913, 1957
BtsI GCAGTG 2 cut(s) 1527, 1815
BtsIMutI CAGTG 8 cut(s) 530, 687, 1210, 1527, 1815, 2032, 2304, 2361
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 6 cut(s) 80, 154, 1191, 1546, 2100, 2320
CfoI GCGC 4 cut(s) 80, 82, 140, 838
Cfr13I GGNCC 4 cut(s) 31, 32, 999, 1149
Cfr42I CCGCGG 1 cut(s) 2166
CseI GACGC 3 cut(s) 352, 1624, 2068
Csp6I GTAC 3 cut(s) 208, 274, 1679
CspCI CAANNNNNGTGG 2 cut(s) 961, 996
CviAII CATG 8 cut(s) 235, 794, 1176, 1381, 1564, 1668, 2107, 2319
CviQI GTAC 3 cut(s) 208, 274, 1679
DdeI CTNAG 9 cut(s) 36, 134, 408, 731, 1611, 1751, 1821, 1950, 2307
DpnI GATC 7 cut(s) 263, 522, 570, 768, 1320, 1488, 2181
DpnII GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
DraI TTTAAA 1 cut(s) 549
DraIII CACNNNGTG 2 cut(s) 958, 1631
EaeI YGGCCR 3 cut(s) 52, 456, 1664
Eam1104I CTCTTC 3 cut(s) 654, 1751, 1980
EarI CTCTTC 3 cut(s) 654, 1751, 1980
EciI GGCGGA 1 cut(s) 1196
Eco130I CCWWGG 5 cut(s) 24, 234, 255, 1380, 1667
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 1 cut(s) 999
Eco57I CTGAAG 6 cut(s) 665, 787, 791, 1824, 1914, 2114
Eco88I CYCGRG 1 cut(s) 1151
EcoO109I RGGNCCY 2 cut(s) 31, 32
EcoRII CCWGG 1 cut(s) 761
EcoT14I CCWWGG 5 cut(s) 24, 234, 255, 1380, 1667
EcoT22I ATGCAT 1 cut(s) 1447
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 5 cut(s) 24, 234, 255, 1380, 1667
Esp3I CGTCTC 2 cut(s) 170, 1607
FaeI CATG 8 cut(s) 238, 797, 1179, 1384, 1567, 1671, 2110, 2322
FaqI GGGAC 2 cut(s) 86, 821
FatI CATG 8 cut(s) 234, 793, 1175, 1380, 1563, 1667, 2106, 2318
FauI CCCGC 1 cut(s) 2103
FblI GTMKAC 1 cut(s) 593
FokI GGATG 3 cut(s) 32, 920, 1944
FriOI GRGCYC 1 cut(s) 35
FspBI CTAG 8 cut(s) 1415, 1529, 1683, 1914, 1919, 2013, 2033, 2312
GlaI GCGC 4 cut(s) 79, 81, 139, 837
GsuI CTGGAG 2 cut(s) 410, 858
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 8 cut(s) 33, 54, 458, 1150, 1379, 1666, 1778, 1935
HapII CCGG 4 cut(s) 51, 169, 347, 2222
HgaI GACGC 3 cut(s) 352, 1624, 2068
HhaI GCGC 4 cut(s) 80, 82, 140, 838
Hin1I GRCGYC 2 cut(s) 144, 344
Hin1II CATG 8 cut(s) 238, 797, 1179, 1384, 1567, 1671, 2110, 2322
Hin6I GCGC 4 cut(s) 78, 80, 138, 836
HinP1I GCGC 4 cut(s) 78, 80, 138, 836
HincII GTYRAC 2 cut(s) 94, 594
HindII GTYRAC 2 cut(s) 94, 594
HindIII AAGCTT 2 cut(s) 542, 1472
HinfI GANTC 8 cut(s) 283, 595, 604, 898, 1283, 1407, 1745, 2065
HpaII CCGG 4 cut(s) 51, 169, 347, 2222
HphI GGTGA 6 cut(s) 64, 751, 970, 1001, 1655, 2246
Hpy166II GTNNAC 3 cut(s) 94, 594, 2030
Hpy188I TCNGA 7 cut(s) 409, 771, 1162, 1318, 1849, 1953, 2070
Hpy8I GTNNAC 3 cut(s) 94, 594, 2030
Hpy99I CGWCG 2 cut(s) 146, 346
HpyAV CCTTC 9 cut(s) 640, 978, 1381, 1430, 1570, 1997, 2065, 2225, 2260
HpyCH4III ACNGT 9 cut(s) 184, 450, 536, 753, 958, 1205, 2020, 2300, 2365
HpyCH4IV ACGT 5 cut(s) 63, 90, 99, 144, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 135, 1195, 1307, 1895, 2085
HpyF3I CTNAG 9 cut(s) 36, 134, 408, 731, 1611, 1751, 1821, 1950, 2307
HpySE526I ACGT 5 cut(s) 63, 90, 99, 144, 479
Hsp92I GRCGYC 2 cut(s) 144, 344
Hsp92II CATG 8 cut(s) 238, 797, 1179, 1384, 1567, 1671, 2110, 2322
HspAI GCGC 4 cut(s) 78, 80, 138, 836
KspI CCGCGG 1 cut(s) 2166
Kzo9I GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
LmnI GCTCC 5 cut(s) 45, 349, 696, 1363, 1505
Lsp1109I GCAGC 9 cut(s) 69, 113, 372, 433, 436, 1484, 1606, 1837, 2128
LweI GCATC 8 cut(s) 823, 1081, 1288, 1297, 1543, 1954, 1960, 1966
MaeI CTAG 8 cut(s) 1415, 1529, 1683, 1914, 1919, 2013, 2033, 2312
MaeII ACGT 5 cut(s) 63, 90, 99, 144, 479
MaeIII GTNAC 6 cut(s) 16, 1021, 1688, 2153, 2212, 2300
MalI GATC 7 cut(s) 263, 522, 570, 768, 1320, 1488, 2181
MboI GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
MflI RGATCY 3 cut(s) 261, 568, 766
MhlI GDGCHC 2 cut(s) 35, 485
MlyI GAGTC 3 cut(s) 589, 1277, 1401
MmeI TCCRAC 3 cut(s) 636, 1054, 1140
Mph1103I ATGCAT 1 cut(s) 1447
MroXI GAANNNNTTC 1 cut(s) 1054
MslI CAYNNNNRTG 3 cut(s) 959, 1508, 1962
MspA1I CMGCKG 2 cut(s) 2165, 2351
MspI CCGG 4 cut(s) 51, 169, 347, 2222
MspR9I CCNGG 4 cut(s) 51, 170, 763, 2223
Mva1269I GAATGC 2 cut(s) 709, 1447
MvaI CCWGG 1 cut(s) 763
MvnI CGCG 2 cut(s) 80, 2165
MwoI GCNNNNNNNGC 5 cut(s) 135, 1195, 1307, 1895, 2085
NciI CCSGG 3 cut(s) 51, 170, 2223
NcoI CCATGG 3 cut(s) 234, 1380, 1667
NdeII GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
NlaIII CATG 8 cut(s) 238, 797, 1179, 1384, 1567, 1671, 2110, 2322
NlaIV GGNNCC 6 cut(s) 33, 199, 214, 570, 698, 783
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 2 cut(s) 1688, 2153
NsiI ATGCAT 1 cut(s) 1447
NspI RCATGY 1 cut(s) 2322
NspV TTCGAA 1 cut(s) 2229
PaeI GCATGC 1 cut(s) 2322
PaqCI CACCTGC 1 cut(s) 283
PauI GCGCGC 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 160
PctI GAATGC 2 cut(s) 709, 1447
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 5 cut(s) 283, 604, 898, 1745, 2065
PleI GAGTC 3 cut(s) 589, 1277, 1401
PpsI GAGTC 3 cut(s) 589, 1277, 1401
PshBI ATTAAT 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 761
PspGI CCWGG 1 cut(s) 761
PspN4I GGNNCC 6 cut(s) 33, 199, 214, 570, 698, 783
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 4 cut(s) 31, 32, 999, 1149
PsrI GAACNNNNNNTAC 2 cut(s) 290, 322
PstI CTGCAG 1 cut(s) 1522
PsuI RGATCY 3 cut(s) 261, 568, 766
PteI GCGCGC 1 cut(s) 78
PvuII CAGCTG 1 cut(s) 2351
RsaI GTAC 3 cut(s) 209, 275, 1680
RsaNI GTAC 3 cut(s) 208, 274, 1679
RseI CAYNNNNRTG 3 cut(s) 959, 1508, 1962
SacII CCGCGG 1 cut(s) 2166
SalI GTCGAC 1 cut(s) 592
Sau3AI GATC 7 cut(s) 261, 520, 568, 766, 1318, 1486, 2179
Sau96I GGNCC 4 cut(s) 31, 32, 999, 1149
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 3 cut(s) 589, 1277, 1401
ScrFI CCNGG 4 cut(s) 51, 170, 763, 2223
SduI GDGCHC 2 cut(s) 35, 485
SfaNI GCATC 8 cut(s) 823, 1081, 1288, 1297, 1543, 1954, 1960, 1966
SfcI CTRYAG 2 cut(s) 1518, 2282
Sfr303I CCGCGG 1 cut(s) 2166
SfuI TTCGAA 1 cut(s) 2229
SgrBI CCGCGG 1 cut(s) 2166
SinI GGWCC 1 cut(s) 999
SmiMI CAYNNNNRTG 3 cut(s) 959, 1508, 1962
SmlI CTYRAG 3 cut(s) 1302, 1829, 2196
SmoI CTYRAG 3 cut(s) 1302, 1829, 2196
SpeI ACTAGT 1 cut(s) 2032
SphI GCATGC 1 cut(s) 2322
SsiI CCGC 6 cut(s) 195, 216, 1181, 2110, 2163, 2165
SspI AATATT 1 cut(s) 313
SspMI CTAG 8 cut(s) 1415, 1529, 1683, 1914, 1919, 2013, 2033, 2312
StyD4I CCNGG 4 cut(s) 49, 168, 761, 2221
StyI CCWWGG 5 cut(s) 24, 234, 255, 1380, 1667
TaaI ACNGT 9 cut(s) 184, 450, 536, 753, 958, 1205, 2020, 2300, 2365
TaiI ACGT 5 cut(s) 66, 93, 102, 147, 482
TaqI TCGA 3 cut(s) 341, 593, 2229
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 5 cut(s) 283, 604, 898, 1745, 2065
TscAI CASTG 8 cut(s) 530, 687, 1210, 1527, 1815, 2032, 2311, 2368
TseFI GTSAC 2 cut(s) 1688, 2153
TseI GCWGC 9 cut(s) 82, 126, 385, 421, 424, 1497, 1594, 1825, 2141
Tsp45I GTSAC 2 cut(s) 1688, 2153
TspDTI ATGAA 7 cut(s) 275, 701, 1022, 1056, 1222, 1552, 1752
TspGWI ACGGA 2 cut(s) 371, 616
TspRI CASTG 8 cut(s) 530, 687, 1210, 1527, 1815, 2032, 2311, 2368
VpaK11BI GGWCC 1 cut(s) 999
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 1 cut(s) 326
XbaI TCTAGA 1 cut(s) 1913
XceI RCATGY 1 cut(s) 2322
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 8 cut(s) 1415, 1529, 1683, 1914, 1919, 2013, 2033, 2312
ZraI GACGTC 1 cut(s) 145
ZrmI AGTACT 1 cut(s) 275
Zsp2I ATGCAT 1 cut(s) 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.