Rorug04G0241900

NADH-ubiquinone oxidoreductase 11 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010973
Physical Location & Seq
Forward (+)
41043835 .. 41045008
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0241900.1

Sequence Viewer

Length: 930 bp
ATGCAGAAGATGTTTGCTCGGAGTCTGAGTTCGCTCTACCGCTCAGGCAGGTCTATCGCCGTCAGCAAGCAGCTGCTCACTCACCCGCCACGTCAGCACTACTTCTCTTACTCCACCGGCGTTGCTGCTCGAACAACCCTCCTCAACTTCTCATCTCCGATGGCCAAGCACCTCTCTTCCGATGATTATGGGTCCCCTCTTTCAAGGGGTTTGGGAAGTAGGAGACTTTTACATGGTGAGAGTTGCAGCAAAGATTATGACGTATATGTACAGCATATGTTTGAAAAGATTCGGGTAGATTTCAATGACAGTCTTGAGAAATTTGCCTTTGAAATGTTTGATTCTATGAGAGATGAGGGGCTGGCCGACGAAGCCAATAAGGTTTTCAAGCCTTTCCTTCAGATAGAGAGCCGAGTGCCAGCCGTGGCGGTTTTCACTTACATGATTGATGGCTTGCTCAATACCGGCCAGACCAACATGGCACTCAACGTTTACAGGTACATGTTAGCCACTGGAGTCACTCCCAGCAGCTACACGTACAATTTACTCGTCGTGGGACTTAGGGTTGCAGCTCAATGGGACGCTAATATGCTTGGTGTTGCCAAGAAGTGTTTTGTGGAAATGTTGGATAAGGGCAGGAAGCCCAATTTTGATGTTTACTGGAGGGTGTTTGAAGGCATTGCCTGTGTGGAGACGGTGGAGGAGGCAATGAAGTTGCATCATTATCTTGAGATGACGGCTGATAAGGACATGCTAAGAGTCTTATGTGACTGGCGCATCCAAGACACCCAGAAACAGGCAGACAATATGTACAAGGCTTTACTTGATGATGGAAATGGACACGAGGCATGGGTGCTCTTTCAGAAGCAGTACCATGAGTCGAGCGTGCTGCGTGCGCCACATGGTTGTCCTGTATACCTGTGTTATTGA

Protein Analysis

309

Amino Acids

35.38

Weight (kDa)

7.13

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 140 - 187 3.8e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 39
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 915
AciI CCGC 3 cut(s) 40, 86, 428
AclI AACGTT 1 cut(s) 489
AcoI YGGCCR 3 cut(s) 162, 363, 466
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 5 cut(s) 270, 500, 539, 812, 872
AfiI CCNNNNNNNGG 1 cut(s) 796
AflIII ACRYGT 2 cut(s) 501, 534
AgsI TTSAA 6 cut(s) 204, 284, 304, 332, 388, 674
AjiI CACGTC 1 cut(s) 92
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
AluBI AGCT 3 cut(s) 73, 531, 572
AluI AGCT 3 cut(s) 73, 531, 572
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 2 cut(s) 217, 686
AoxI GGCC 3 cut(s) 162, 363, 466
ApeKI GCWGC 7 cut(s) 70, 73, 125, 246, 528, 569, 889
ApoI RAATTY 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 2 cut(s) 777, 898
AspS9I GGNCC 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 74, 248
AvaII GGWCC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 164
BauI CACGAG 1 cut(s) 842
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 7 cut(s) 60, 82, 112, 258, 540, 581, 876
BccI CCATC 3 cut(s) 154, 443, 824
BceAI ACGGC 3 cut(s) 44, 407, 753
BcgI CGANNNNNNTGC 4 cut(s) 37, 71, 871, 905
BcoDI GTCTC 2 cut(s) 217, 686
BfuAI ACCTGC 1 cut(s) 39
BisI GCNGC 7 cut(s) 71, 74, 126, 247, 529, 570, 890
BlsI GCNGC 7 cut(s) 72, 75, 127, 248, 530, 571, 891
Bme18I GGWCC 1 cut(s) 192
BmgBI CACGTC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 2 cut(s) 193, 194
BmsI GCATC 2 cut(s) 727, 786
BpmI CTGGAG 2 cut(s) 534, 682
Bpu10I CCTNAGC 1 cut(s) 43
BpuEI CTTGAG 2 cut(s) 335, 749
BsaAI YACGTR 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 796
Bse118I RCCGGY 2 cut(s) 116, 464
Bse1I ACTGG 3 cut(s) 517, 665, 776
Bse3DI GCAATG 2 cut(s) 678, 714
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 1 cut(s) 777
BseLI CCNNNNNNNGG 1 cut(s) 796
BseMI GCAATG 2 cut(s) 678, 714
BseMII CTCAG 2 cut(s) 17, 57
BseNI ACTGG 3 cut(s) 517, 665, 776
BseRI GAGGAG 2 cut(s) 131, 716
BseXI GCAGC 7 cut(s) 60, 82, 112, 258, 540, 581, 876
BseYI CCCAGC 1 cut(s) 524
BshFI GGCC 3 cut(s) 164, 365, 468
BsiHKAI GWGCWC 1 cut(s) 858
BsiSI CCGG 2 cut(s) 117, 465
BslFI GGGAC 3 cut(s) 178, 570, 593
BslI CCNNNNNNNGG 1 cut(s) 796
BsmAI GTCTC 2 cut(s) 217, 686
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 3 cut(s) 178, 570, 593
BsnI GGCC 3 cut(s) 164, 365, 468
Bsp1286I GDGCHC 1 cut(s) 858
Bsp1407I TGTACA 2 cut(s) 268, 810
BspACI CCGC 3 cut(s) 40, 86, 428
BspANI GGCC 3 cut(s) 164, 365, 468
BspCNI CTCAG 2 cut(s) 18, 56
BspLI GGNNCC 2 cut(s) 193, 194
BspMI ACCTGC 1 cut(s) 39
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 678, 714
BsrFI RCCGGY 2 cut(s) 116, 464
BsrGI TGTACA 2 cut(s) 268, 810
BsrI ACTGG 3 cut(s) 517, 665, 776
BssAI RCCGGY 2 cut(s) 116, 464
BssECI CCNNGG 1 cut(s) 423
BssNAI GTATAC 1 cut(s) 916
BssSI CACGAG 1 cut(s) 842
Bst1107I GTATAC 1 cut(s) 916
Bst2BI CACGAG 1 cut(s) 842
Bst4CI ACNGT 2 cut(s) 311, 697
Bst6I CTCTTC 1 cut(s) 181
BstAUI TGTACA 2 cut(s) 268, 810
BstBAI YACGTR 1 cut(s) 537
BstC8I GCNNGC 6 cut(s) 68, 363, 420, 455, 887, 894
BstDEI CTNAG 4 cut(s) 26, 43, 560, 755
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 777
BstHHI GCGC 2 cut(s) 777, 898
BstMAI GTCTC 2 cut(s) 217, 686
BstMWI GCNNNNNNNGC 3 cut(s) 94, 371, 895
BstNSI RCATGY 2 cut(s) 505, 754
BstV1I GCAGC 7 cut(s) 60, 82, 112, 258, 540, 581, 876
BstZ17I GTATAC 1 cut(s) 916
BsuRI GGCC 3 cut(s) 164, 365, 468
BtgI CCRYGG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 777
BtsIMutI CAGTG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 6 cut(s) 68, 363, 420, 455, 887, 894
CfoI GCGC 2 cut(s) 777, 898
Cfr10I RCCGGY 2 cut(s) 116, 464
Cfr13I GGNCC 1 cut(s) 192
CseI GACGC 1 cut(s) 590
Csp6I GTAC 5 cut(s) 269, 499, 538, 811, 871
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 8 cut(s) 233, 442, 478, 502, 751, 849, 875, 902
CviQI GTAC 5 cut(s) 269, 499, 538, 811, 871
DdeI CTNAG 4 cut(s) 26, 43, 560, 755
EaeI YGGCCR 3 cut(s) 162, 363, 466
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 686
FaeI CATG 8 cut(s) 236, 445, 481, 505, 754, 852, 878, 905
FaqI GGGAC 3 cut(s) 178, 570, 593
FatI CATG 8 cut(s) 232, 441, 477, 501, 750, 848, 874, 901
FauI CCCGC 1 cut(s) 93
FauNDI CATATG 1 cut(s) 276
FblI GTMKAC 1 cut(s) 915
Fnu4HI GCNGC 7 cut(s) 71, 74, 126, 247, 529, 570, 890
FokI GGATG 1 cut(s) 764
Fsp4HI GCNGC 7 cut(s) 71, 74, 126, 247, 529, 570, 890
GlaI GCGC 2 cut(s) 776, 897
GluI GCNGC 7 cut(s) 71, 74, 126, 247, 529, 570, 890
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 2 cut(s) 534, 682
HaeIII GGCC 3 cut(s) 164, 365, 468
HapII CCGG 2 cut(s) 117, 465
HgaI GACGC 1 cut(s) 590
HhaI GCGC 2 cut(s) 777, 898
Hin1II CATG 8 cut(s) 236, 445, 481, 505, 754, 852, 878, 905
Hin6I GCGC 2 cut(s) 775, 896
HinP1I GCGC 2 cut(s) 775, 896
HinfI GANTC 6 cut(s) 22, 289, 341, 516, 759, 878
HpaII CCGG 2 cut(s) 117, 465
HphI GGTGA 2 cut(s) 74, 248
Hpy166II GTNNAC 3 cut(s) 493, 658, 916
Hpy188I TCNGA 6 cut(s) 21, 27, 159, 181, 402, 864
Hpy188III TCNNGA 2 cut(s) 314, 728
Hpy8I GTNNAC 3 cut(s) 493, 658, 916
Hpy99I CGWCG 2 cut(s) 371, 554
HpyAV CCTTC 2 cut(s) 407, 668
HpyCH4III ACNGT 2 cut(s) 311, 697
HpyCH4IV ACGT 4 cut(s) 91, 261, 489, 536
HpyCH4V TGCA 4 cut(s) 4, 246, 569, 718
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 371, 895
HpyF3I CTNAG 4 cut(s) 26, 43, 560, 755
HpySE526I ACGT 4 cut(s) 91, 261, 489, 536
Hsp92II CATG 8 cut(s) 236, 445, 481, 505, 754, 852, 878, 905
HspAI GCGC 2 cut(s) 775, 896
KflI GGGWCCC 1 cut(s) 192
Lsp1109I GCAGC 7 cut(s) 60, 82, 112, 258, 540, 581, 876
LweI GCATC 2 cut(s) 727, 786
MaeII ACGT 4 cut(s) 91, 261, 489, 536
MaeIII GTNAC 2 cut(s) 517, 767
MbiI CCGCTC 1 cut(s) 42
MboII GAAGA 2 cut(s) 19, 168
MhlI GDGCHC 1 cut(s) 858
MlsI TGGCCA 1 cut(s) 164
MluCI AATT 3 cut(s) 320, 541, 646
MluNI TGGCCA 1 cut(s) 164
MlyI GAGTC 4 cut(s) 31, 525, 768, 887
MmeI TCCRAC 1 cut(s) 606
MnlI CCTC 9 cut(s) 149, 152, 182, 207, 349, 657, 694, 697, 838
Mox20I TGGCCA 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 288
MscI TGGCCA 1 cut(s) 164
MslI CAYNNNNRTG 1 cut(s) 440
Msp20I TGGCCA 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 2 cut(s) 117, 465
MwoI GCNNNNNNNGC 3 cut(s) 94, 371, 895
NdeI CATATG 1 cut(s) 276
NlaIII CATG 8 cut(s) 236, 445, 481, 505, 754, 852, 878, 905
NlaIV GGNNCC 2 cut(s) 193, 194
NmeAIII GCCGAG 1 cut(s) 437
NmuCI GTSAC 2 cut(s) 517, 767
NspI RCATGY 2 cut(s) 505, 754
PciI ACATGT 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 289, 341
PkrI GCNGC 7 cut(s) 72, 75, 127, 248, 530, 571, 891
PleI GAGTC 4 cut(s) 30, 524, 767, 886
PpsI GAGTC 4 cut(s) 30, 524, 767, 886
Ppu21I YACGTR 1 cut(s) 537
PpuMI RGGWCCY 1 cut(s) 192
PscI ACATGT 1 cut(s) 501
Psp1406I AACGTT 1 cut(s) 489
Psp5II RGGWCCY 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 524
PspN4I GGNNCC 2 cut(s) 193, 194
PspPI GGNCC 1 cut(s) 192
PspPPI RGGWCCY 1 cut(s) 192
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 5 cut(s) 270, 500, 539, 812, 872
RsaNI GTAC 5 cut(s) 269, 499, 538, 811, 871
RseI CAYNNNNRTG 1 cut(s) 440
SatI GCNGC 7 cut(s) 71, 74, 126, 247, 529, 570, 890
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 4 cut(s) 31, 525, 768, 887
SduI GDGCHC 1 cut(s) 858
SfaNI GCATC 2 cut(s) 727, 786
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 314, 728
SmoI CTYRAG 2 cut(s) 314, 728
Sse9I AATT 3 cut(s) 320, 541, 646
SsiI CCGC 3 cut(s) 40, 86, 428
TaaI ACNGT 2 cut(s) 311, 697
TaiI ACGT 4 cut(s) 94, 264, 492, 539
TaqI TCGA 2 cut(s) 130, 881
TasI AATT 3 cut(s) 320, 541, 646
TatI WGTACW 2 cut(s) 268, 810
TfiI GAWTC 2 cut(s) 289, 341
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 2 cut(s) 517, 767
TseI GCWGC 7 cut(s) 70, 73, 125, 246, 528, 569, 889
Tsp45I GTSAC 2 cut(s) 517, 767
TspDTI ATGAA 1 cut(s) 725
TspRI CASTG 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 1 cut(s) 320
XceI RCATGY 2 cut(s) 505, 754
XmiI GTMKAC 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.